Tecan Sunrise Service manual

Technical Manual for

SUNRISE RC, TW, TS, TC, BC

Document Part No. T 137 302

February 2002

Document Revision: 1.0

Firmware Revision Level: 3.xx

TECAN Affiliates and Service Centers

Austria		Asia
Tecan Austria GmbH		Tecan Asia (Pte) Ltd.
Untersbergstrasse 1a		80, Marine Parade #13-04
A-5082 Grödig / Salzburg		Singapore 449269
Austria		Singapore
Tel.: +43	62 46 89 33	Tel.: [phone]
Fax: +43	62 46 72 770	Fax: [phone]

Switzerland	Belgium
Tecan Schweiz AG	Tecan Benelux B.V.B.A.
Seestrasse 103	Vaartdijk 55
CH-8708 Männedorf	B-2800 Mechelen
Switzerland	Belgium
Tel.: + 41 1 922 81 11	Tel.: [phone]
Fax. : [phone]	Fax. [phone]

France	Italy
Tecan France S.A.	Tecan Italia S.r.l.
Parc d'Activités de Pissaloup	Via F.lli Cervi
Batiment Hermes II	Palazzo Bernini
Rue Edouard Branly	Centro Direzionale Milano2
F-78190 Trappes	20090 Segrate (Mi)
France	Italy
Tel.: [phone]	Tel.: +39	02 215 21 28
Fax: [phone]	Fax: +39	02 215 97 441

Japan	USA
Tecan Japan Co. Ltd	Tecan US
Meiji Seimei Fuchu Building 10F	P.O. Box 13953
1-40 Miyamachi	Research Triangle Park,
Fuchu City, Tokyo	NC 27709
Japan	USA
Tel.: [phone]	Tel.: [phone]
Fax: [phone]	Fax: [phone]

Spain	Germany
Tecan Spain	Tecan Deutschland GmbH
Sabino de Arana, 32	Theodor-Strom-Straße 17
E-08028 Barcelona	D-74564 Crailsheim
Spain	Germany
Tel.: [phone]	Tel.: +49	79 51 94 170
Fax: [phone]	Fax: +49	79 51 50 38

USA	Netherlands
Tecan Boston	Tecan Benelux B.V.B.A.
200 Boston Avenue	Industrieweg 30,
Suite 3000	NL-4283 Giessen,
Medford, MA 02155	Netherlands;
USA	Tel.: [phone]
Tel.: [phone]	Fax: [phone]
Fax: [phone]
United Kingdom
Tecan UK
Theale Court
11-13 High Street
Theale
UK-Reading RG7 5AH
United Kingdom
Tel.: [phone]
Fax: [phone]

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Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0

February 2002

WARNING

CAREFULLY READ AND FOLLOW THE INSTRUCTIONS

PROVIDED IN THIS MANUAL BEFORE OPERATING THE

INSTRUMENT.

Notice

Every effort has been made to avoid errors in text and diagrams, however, TECAN Austria Ges.m.b.H. assumes no responsibility for any errors which may appear in this publication.

It is the policy of TECAN Austria Ges.m.b.H. to improve products as new techniques and components become available.TECAN Austria Ges.m.b.H. therefore reserves the right to change specifications at any time.

We would appreciate any comments on this publication. TECAN Austria Ges.m.b.H.

Untersbergstraße 1A A-5082 Grödig/Salzburg AUSTRIA / EUROPE

Telephone: [phone] FAX: [phone] E-mail: [e-mail]

Copyright Information

The contents of this manual are the property of TECAN Austria Ges.m.b.H and are not to be copied, reproduced or transferred to another person or persons without our prior written permission.

Copyright TECAN Austria Ges.m.b.H All rights reserved.

Printed in Austria.

February 2002

Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0

3

Warnings, Cautions and Notes

There are three types of informational notices used in this manual. These notices highlight important information or warn the user of a potentially dangerous situation. The following notices are:

Note: Gives helpful information.

Caution

Indicates a possibility of instrument damage or data loss if instructions are not followed.

WARNING

INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY,

LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS

ARE NOT FOLLOWED.

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Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0

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Table of Contents

1. General
1.1	Introduction ...............................................................................	1-1
	Abbreviations:..............................................................................	1-1
1.1.1	Available Options for SUNRISE ..................................................	1-2
1.2	Instrument Description .............................................................	1-3
1.2.1	Remote Control ...........................................................................	1-3
1.2.2	Touchscreen................................................................................	1-3
1.2.3	Back Panel Connections .............................................................	1-4
1.3	Filter Carriage Description .......................................................	1-5
1.3.1	SUNRISE Standard Filter Carriage .............................................	1-5
1.3.2	Sunrise Tuneable Wavelength Filter Carriage.............................	1-5
1.3.3	Software Features .......................................................................	1-6
1.4	Instrument Features..................................................................	1-6
1.4.1	Measurement Modes...................................................................	1-6
1.4.2	Microplate Shaking......................................................................	1-7
1.5	Instrument Accessories............................................................	1-7
	Computer Software for Personal Computer ................................	1-7

2.	Installation Procedure
	2.1	Introduction ...............................................................................	2-1
	2.2	Unpacking and Inspection........................................................	2-1
	2.2.1	Unpacking Procedure..................................................................	2-2
	2.3	Power Requirements.................................................................	2-2
	2.4	Environmental Requirements...................................................	2-2
	2.5	Instrument Installation Procedure ...........................................	2-3
	2.5.1	Installation of Instrument Control Software..................................	2-3
3. Firmware and Software Description
	3.1	Rdr Download Software............................................................	3-1
	3.1.1	Starting the Firmware Download .................................................	3-2
	3.1.2	Open file ......................................................................................	3-3
	3.2	Rdr OLE Server..........................................................................	3-4
	3.2.1	Installation of Instrument Control Software..................................	3-4
	3.3	Sunrise Instrument Settings ....................................................	3-4
	3.3.1	Installation of SUNRISE Instrument Settings Software ...............	3-4
	3.3.2	Starting the SUNRISE Instrument Settings .................................	3-5
	3.3.3	Define Instrument Mode ..............................................................	3-5
	3.3.4	Define Filter .................................................................................	3-6
	3.3.5	Define Measurement Mode .........................................................	3-7
	3.4	SunSet Software (Service tool kit is necessary!) ...................	3-8
	3.4.1	Installation of SUNSET Software.................................................	3-8
	3.4.2	Starting the SunSet Software ......................................................	3-8
	3.4.3	Device configuration..................................................................	3-10
	3.4.4	Mirror and lamp adjustment.......................................................	3-10
	3.4.5	Plate calibration.........................................................................	3-11
	3.4.6	Measurement offset (Inhouse plate is necessary).....................	3-12
	3.4.7	User configuration .....................................................................	3-13
	3.4.8	SunSet Options Menu ...............................................................	3-14
		Filter control...............................................................................	3-14
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	Plate Movements.......................................................................	3-15
	Duration test ..............................................................................	3-15
3.5	Sunrise Diagnosis Tool ..........................................................	3-16
3.5.1	Installation of Sunrise Diagnosis Tool .......................................	3-16
3.5.2	Connect instrument ...................................................................	3-17
3.5.3	Get Diagnosis Information.........................................................	3-18
3.5.4	Duration test ..............................................................................	3-18
3.5.5	Print report.................................................................................	3-19
3.6	Error messages .......................................................................	3-19

4. Removing and Replacing the Instrument Cover

4.1	Removal of Instrument Top Cover...........................................	4-1
4.2	Removal of the Front Cover .....................................................	4-3
4.3	Replacing the Front Cover .......................................................	4-4
4.4	Replacing the instrument top cover ........................................	4-4
4.5	Removal of Touchscreen..........................................................	4-5

5. Optical System

5.1	Introduction ...............................................................................	5-1
5.2	Optical System Description......................................................	5-1
5.3	Optical System Diagram ...........................................................	5-2
	SUNRISE ST (Option Standard Optic) Instrument......................	5-2
	SUNRISE TW (Tuneable Wavelength Optic) Instrument ............	5-2
5.3.1	Measurements with Wavelengths below 400 nm ........................	5-3
5.3.2	Agglutination Measurement Mask for SUNRISE ST Option........	5-3
5.4	Lamp Replacement ...................................................................	5-4
5.5	Filter Slide ..................................................................................	5-5
5.5.1	SUNRISE Filter Slide (standard filter slide).................................	5-5
5.5.2	Replacement of Filters in SUNRISE Filter Slides........................	5-6
5.5.3	Filter Coding for SUNRISE Filter Slides ......................................	5-7
5.5.4	Definition of the filter slide codes:................................................	5-8
	Slide D.........................................................................................	5-8
5.6	Filter slide ( Tuneable wavelength)..........................................	5-9
5.7	Lamp and Mirror Unit Parts ....................................................	5-11
5.7.1	Heat Filter and Air Filter ............................................................	5-11
5.7.2	Mirror, Optical Lens ...................................................................	5-12
	For Mirror Replacement: ...........................................................	5-12
	For Optical Lens Replacement..................................................	5-13
5.8	Filter Guide (standard instrument ) .......................................	5-13
5.9	Filter Guide ( Tuneable Wavelength):....................................	5-15
5.10	Fiber Bundle ............................................................................	5-16

5.10.1Difference between Standard Fiber Optic and Tuneable Wavelength

	Fiber Optic.................................................................................	5-17
5.11	Further Parts belonging to the Optical System....................	5-17
5.12	Blocking Filter Wheel for Tuneable Wavelength ..................	5-18
5.13	Filter Wheel Properties ...........................................................	5-19
5.14	Mirror for Barcode Scanner....................................................	5-19

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6. Mechanical System
6.1	Introduction ...............................................................................	6-1
6.2	Transport System Description .................................................	6-1
6.3	Measurement Procedure ..........................................................	6-1
	Inside Shaking.............................................................................	6-2
6.4	The Transport Box Assembly ..................................................	6-2
6.5	Exchanging the Transport Box ................................................	6-3
6.6	Transport Box Adjustments .....................................................	6-5
6.7	Replacing the Temperature control unit .................................	6-5

7. Electronic System
7.1	Introduction ...............................................................................	7-1
7.2	Interconnection diagram (standard RC)..................................	7-2
7.3	Voltage diagram (standard RC)................................................	7-3
7.4	Main Board.................................................................................	7-4
7.5	Replacing the Main Board ........................................................	7-5
7.6	Power Board (standard)............................................................	7-7
7.7	Exchanging the Power Board (standard)................................	7-8
7.8	Power Board (tuneable wavelength)........................................	7-9
7.9	TW Board ................................................................................	7-11
7.10	Thermo Board..........................................................................	7-12
7.11	12 Volt Board ...........................................................................	7-13
7.12	Power Supply board................................................................	7-14
7.13	Replacing the Power Supply ..................................................	7-15
7.14	Barcode Board.........................................................................	7-16
7.15	Optical Switches......................................................................	7-17

8.	Interfaces
	8.1	Computer Interface ...................................................................	8-1
	8.1.1	Hardware Specifications..............................................................	8-1
	8.1.2	Pin Designation ...........................................................................	8-1
	8.1.3	RS-232-C Interface Lines............................................................	8-2
		Requirements for the Serial Interface:.........................................	8-2
		Description of the serial Interface:...............................................	8-3
		Implemented RS-232-C Interface Lines : ....................................	8-3
		Available Setups:.........................................................................	8-3
	8.2	Handshakes: ..............................................................................	8-4
		Software Handshake: ..................................................................	8-4
	8.3	Commands Response Time: ....................................................	8-4
	8.4	Command Syntax for the Serial Interface:..............................	8-4
		Sunrise Mode (Standard): ...........................................................	8-4
		Spectra Mode (for compatibility):................................................	8-4
	8.5	Synchronization and Data Format ...........................................	8-5
	8.6	Signal Levels .............................................................................	8-5
	8.7	Software Specifications............................................................	8-6
	8.8	Handshake .................................................................................	8-6
	8.9	Timing Of Messages .................................................................	8-6
	8.10	Communication Messages .......................................................	8-6
	8.11	Printer Interface.........................................................................	8-7
	8.11.1	Character Set Setting ..................................................................	8-7
	8.11.2	Hardware Specifications..............................................................	8-7
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8.11.3	Connector....................................................................................	8-7
8.12	Parallel Interface Lines .............................................................	8-8
8.13	Table of Connections................................................................	8-8
8.14	Key to Table ...............................................................................	8-9
	Connector Pin Signals.................................................................	8-9
	Strobe..........................................................................................	8-9
	Data 1 - Data 8 ............................................................................	8-9
	Acklng - Acknowledge .................................................................	8-9
	Busy ............................................................................................	8-9
8.15	Connection with Touchscreen interface connector.............	8-10
8.15.1	XChange Files:..........................................................................	8-10

9. Sunrise Service Tool Kits for Sunrise RC and Options

9.1	Service tool kit 1 (S 039 341) ....................................................	9-1
9.1.1	Introduction..................................................................................	9-1
9.2	Installation Procedure...............................................................	9-2
9.3	Sunrise Diagnosis Software.....................................................	9-2
9.3.1	Explanation of the Output of the Sunrise Diagnosis Tool...........	9-3
9.4	Sunrise SUNSET Software .......................................................	9-6
9.5	QC Pac 2 Service software:......................................................	9-7
9.6	Flashcard Download (S039351) ...............................................	9-8
9.7	Flashcard for Touchscreen unit...............................................	9-8
9.8	Barcode Upgrade Kit.................................................................	9-9
9.8.1	General........................................................................................	9-9
9.8.2	Installation .................................................................................	9-10
9.8.3	Hardware Installation.................................................................	9-12
9.8.4	Software Installation and Adjustment ........................................	9-15
9.9	Overview of Single commands: .............................................	9-22

10. Maintenance

10.1	Instrument Disinfection ..........................................................	10-1
10.2	Disinfection Solutions ............................................................	10-1
10.3	Disinfection Procedure ...........................................................	10-2
10.4	Disinfection Certificate ...........................................................	10-2

10.5Preventative Maintenance Plan for SUNRISE Instruments .10-3

10.5.1	Daily ..........................................................................................	10-3
10.5.2	Weekly.......................................................................................	10-3
10.5.3	Every Six Months ......................................................................	10-3
10.5.4	Yearly (Service Technician Required).......................................	10-3
10.5.5	Every Four Years ......................................................................	10-3
10.6	Electrostatic Discharge Information......................................	10-4
10.6.1	Generating Static.......................................................................	10-4
10.6.2	Preventing Electrostatic Damage to Equipment........................	10-5
10.6.3	Personal Grounding Methods....................................................	10-5

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11. Performance Testing / Quality Control

11.1	Introduction .............................................................................	11-1
11.2	Operating for Maximum Performance ...................................	11-1
11.2.1	Instrument Location...................................................................	11-1
11.2.2	Operating Procedure .................................................................	11-2
11.2.3	Self Check Procedure ...............................................................	11-2
11.3	Performance Tests ..................................................................	11-2
11.3.1	QC PAC 2..................................................................................	11-3
11.3.2	Microplate Test..........................................................................	11-3
11.3.3	High Meniscus Liquids ..............................................................	11-4
	Agglutination Method.................................................................	11-5
	Manual Method..........................................................................	11-6
	Example ....................................................................................	11-6
11.4	Quality Control Testing...........................................................	11-7
11.4.1	Precision Testing.......................................................................	11-7
11.4.2	Instrument Accuracy..................................................................	11-8
11.4.3	Instrument Linearity...................................................................	11-9

12. Trouble Shooting

12.1 Introduction .............................................................................12-1

13. Parts for Sunrise

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1. General

1. General

1.1 Introduction

SUNRISE Remote Control is intended for use with only external software.

Sunrise with TS Option is intended for use with external software and in Stand alone mode.

The SUNRISE instruments are fully automatic, microprocessor controlled readers designed for professional use, enabling the user to measure the light absorbency (optical density) of samples in 96 well microplates according to the specifications described in this manual.

Abbreviations:

RC	Remote control
TW	Tuneable wavelength option
TS	Touchscreen option
TC	Temperature control option
BC	Barcode option

Results obtained using the SUNRISE are influenced by the proper use of the instrument, according to the instructions given in this manual, as well as the liquid compounds used (reagents, chemistry). The instructions for use, storage and other manipulations in connection with samples or reagents have to be strictly followed. Taking this fact into consideration, results must be interpreted carefully.

By reading twelve wells simultaneously, the instrument is able to measure a microplate in approximately eight seconds using the dual wavelength method.

With an innovative range of options, this versatile MTP reader gives diagnostic and research laboratories all the features for numerous purposes.

Based on a new design concept EPAC, the excellent optical performance and high quality of the SUNRISE will guarantee fast, reproducible and accurate measurements.

The SUNRISE is designed to be fitted into TECAN robotic systems.

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1. General

1.1.1Available Options for SUNRISE

The Sunrise is a modular system, so you can create your own tailor-made instrument that meets exactly your needs. Options such as a touch screen combined with a WindowsCE based on-board software, free wavelength selection, temperature control and a bar code scanner can be added onto the basic system which is dedicated to remote controlled computer operation.

Touchscreen

B037302

SUNRISE

Remote Controlled

Tuneable wavelength from 400 – 700 nm

B037306

Temperature control

B037303

Barcode scanner

B037304

CAUTION

IF THE INSTRUCTIONS GIVEN IN THIS MANUAL ARE NOT CORRECTLY CARRIED OUT, THE INSTRUMENT MAY EITHER BECOME DAMAGED OR MAY NO LONGER BE ABLE TO PERFORM ITS PROCEDURES CORRECTLY AND THE ACCURACY OF THE INSTRUMENT

CAN NO LONGER BE GUARANTEED.

For more information about the operating instructions, see Magellan or XRead Plus manuals.

The available options are Retro-fit by TECAN Austria only.*

FOLLOW LASER SPECIFICATIONS (see 1.3 back panel connections)

*Except Barcode Upgrade Kit S 039 386 Only by TECAN certified FSE

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1. General

1.2 Instrument Description

The illustrations below show the components of the instruments.

1.2.1Remote Control

	Power ON LED
	Lamp Compartment
Plate Support	Cartridge and Filter Block Compartment

1.2.2Touchscreen

Touchscreen

Sunrise

Memo Card

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1. General

1.2.3Back Panel Connections

The illustration below shows the connections located in the back panel of the instrument.

BarCode Laser Scanner labels ( only with the barcode option )

			CAUTION				LASERSTRAHLUNG
							NICHT IN DEN STRAHL BLICKEN
							LASER KLASSE 2
				LASER RADIATION -			Leistung: <1mW
							Wellenlänge: 675nm
							EN60825-1:1994+A11:1996
				DO NOT STARE INTO BEAM
				CLASS II LASER PRODUCT
				Complies with 21CFR
				and 1040.10 and 1040.11
Instruments Main Fuses
ON / OFF Switch	I	LISTED					RS 232
	0
		UR L C		UR L	TECAN	PRINTER
Mains Socket		8C64
		LABORATORY EQUIPMENT
		2 x F2A

TECAN	A-5082 AUSTRIA
	TECAN

TYPE	Sunrise
ART.-NR.	F0393xx	VA	110
SER.-NR.	xxxxx	Hz	50 / 60
VOLTAGE AC 100 - 120 / 220 - 240			MADE IN
M-CODE	xxxxxx
			AUSTRIA

Printer Connector	Serial RS 232 Connector
( Only functions with
the touch screen option )

All connected devices must be approved and listed as per EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices

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1. General

1.3 Filter Carriage Description

The SUNRISE instrument can use the following types of filter carriages:

SUNRISE Standard and SUNRISE Gradient Filter (with tuneable wavelength option).

1.3.1SUNRISE Standard Filter Carriage

The SUNRISE standard filter carriage is fitted with up to four narrow band interference filters which have a fixed wavelength.

SUNRISE Standard Filter Carriage

When a wavelength is selected, the entered wavelength is compared against the list of entered filter values for this filter carriage.

If the required filter is fitted in the filter carriage, the filter carriage is moved so that the required filter is in the light beam.

For more information about the definition of the new and customized filter slides, see 3.8 Define Filter.

1.3.2Sunrise Tuneable Wavelength Filter Carriage

•Over 300 wavelengths available from one Gradient filter slide.

•Complete Spectrum scanning - useful for screening new or unknown compounds

•UV light range available on standard filter slide

•High flexibility in one instrument

SUNRISE TW functions only with the Blocking Filter Wheel

See Chapter 5.12 Blocking Filter Wheel for Tuneable Wavelength.

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1. General

1.3.3Software Features

SUNRISE Remote Control is intended for use with only external software.

Sunrise Touchscreen is a stand alone device, that works with windows CE.

For more information about the software features, see the appropriate individual manuals. For example: refer to the Magellan Reference manual.

1.4 Instrument Features

Microplates can be measured using the following features:

•Various measurement modes

•Single or dual wavelength measurements

•Microplate shaking

•Temperature controlled Incubation

1.4.1Measurement Modes

The instrument can be set to use the following measurement modes:

Fast	Plate transport is moved quickly under the measurement diodes so
	that a fast measurement is obtained. (Default setting).
Accurate	Plate transport is moved slowly under the measurement diodes so
	that a very accurate measurement is obtained.
Center	This option measures the optical density only at the center of the
	well.

With the Fast and Accurate measurement modes, the optical density is measured at three positions across the wells and the average measured optical density value from the three measurements is used as the optical density of the well.

The accurate measurement cycle should always be used when measuring high optical densities.

The Center measurement mode should be used if the liquid in the microplate produces a high meniscus, as an incorrect optical density could be obtained if the optical density is measured at three positions. If an Agglutination measurement is performed, all the measurement positions are used.

For more information about setting the measurement mode, see 3.9 Defining the SUNRISE Instrument Settings.

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1. General

1.4.2Microplate Shaking

The SUNRISE is able to shake the microplate before it is measured. Use external software (for example: Magellan) to set the shaking modes.

The microplate can also be shaken between each of the kinetic measurement cycles.

When using a 96 well plate, spillage may occur if the wells are filled with more than 300µl, while using high shaking mode.

1.5 Instrument Accessories

The table below contains the order numbers for instrument accessories:

Part Name	Part Order Number
Halogen lamp.................................................................................	3	709 008
Reader to external computer cable................................................	3	350 005
QC Pac 2 for SUNRISE and SPECTRA ........................................	B 037 358
Additional filter slide .......................................................................	B 036 301
Memory card .............................................................................	B 037 359 01
Flash card for Magellan Windows CE (English) ............................	S 039 351
TS - Pen .........................................................................................	B 037 360
Barcode Upgrade Kit......................................................................	S 039 386
Sunrise Service tool kit ..................................................................	S 039 341

Computer Software for Personal Computer

Related Software

Part of Reader PC Package:

Software	Functionality
Magellan	Instrument control and data reduction
XRead Plus	Instrument control and transfer of raw data to
	Excel.
SUNRISE instrument setting	Enables settings of SUNRISE instrument
	(SUNRISE, SPECTRA, ATC mode and so on).
RDR Download	Enables Download of new firmware from PC to
	reader.
SUNRISE Error Diagnosis	Creates printout of instrument status for service
	purposes.
Sunset Software	For servicing the sunrise and performing all
	instrument adjustments
	(only for trained/ certified persons!)

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2. Installation Procedure

2. Installation Procedure

2.1 Introduction

This chapter contains the necessary information for installing the instrument.

The installation procedures involve unpacking, environmental requirements, power requirements and interfacing.

2.2 Unpacking and Inspection

The delivered instrument is shipped in one carton, which includes: Power cable

Computer connection cable

SUNRISE Operating manual, XRead Plus manual Spare fuses

A software CD, which also contains the XRead Plus program and Magellan demo program (30 day working license).

Accessory box

Foam Packaging	Foam
	Packaging
	Instrument

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2-1

2. Installation Procedure

2.2.1Unpacking Procedure

1.Visually inspect the container for damage, before opening it.

Report any damage immediately.

2.Place the carton in an upright position and open it.

The Cartridge and Filter Block Compartment is fixed with adhesive tape.

3.Lift the instrument out of the carton and place it on a flat surface, free from dust, vibration and away from direct sunlight.

4.Visually inspect the instrument for loose, bent or broken parts.

Report any damage immediately.

5.Compare the instrument's serial number, attached on the rear panel of the instrument, against the serial number of the instrument, on the delivery (shipping) note.

6.Check the instrument accessories against the delivery (shipping) note.

7.Open the plate support area cover and remove the foam strip that is used as the microplate transport lock.

8.Please save all packing materials, as it may be required for later transportation.

2.3Power Requirements

The instrument is auto sensing for the supplied voltage, and therefore does not have to be set for the correct voltage.

Connect the instrument only to a electricity supply system with protective earth.

WARNING

TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE

REPLACED WITH THE SAME TYPE AND RATING OF FUSES.

2.4 Environmental Requirements

The instrument should be placed on a flat, level surface that is free from dust, solvents and acidic vapors.

Vibration and direct sunlight must be avoided, to ensure correct results.

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2. Installation Procedure

2.5 Instrument Installation Procedure

The following procedures detail the necessary steps to be followed when installing the instrument.

WARNING

BEFORE THE INSTRUMENT IS INSTALLED AND SWITCHED ON, IT SHOULD BE LEFT TO STAND FOR AT LEAST THREE HOURS, SO THERE IS NO POSSIBILITY OF CONDENSATION CAUSING A SHORT CIRCUIT.

When the requirements above have been met, installation is carried out using the following procedure:

1.Place the instrument into the required position.

Ensure that the distance between the back panel of the instrument and the wall, is at least 10 cm.

2.Connect the instrument to the external computer with the required interfacing cable.

The interfacing cable is connected into the 9 pin serial interface socket, in the back panel.

3.Ensure that the mains power switch in the back panel of the instrument is in the off position.

4.Insert the power cable into the mains power socket in the back panel.

5.Switch the instrument on using the mains power switch in the back panel. The instrument is now ready to measure microplates.

2.5.1Installation of Instrument Control Software

For more information about installing the software, see Magellan or XRead Plus manual, which can be found on the TECAN Reader PC Package CD.

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3. Firmware and Software Description

3. Firmware and Software

Description

3.1 Rdr Download Software

This program enables the user to Download a new Firmware:

The Rdr Download software is installed using the following procedure:

•Insert TECAN Reader PC package CD into the required CD ROM drive.

•The Main menu is displayed. Click the Software button. Click the Setup button for the Firmware Download. The installation program is started.

•A series of dialog boxes will appear, read each one, enter any necessary information and click Next to continue. The files are then installed and the program icon is created.

•When the Installation Complete dialog box appears, click Finish and the Firmware Download program is ready to be used.

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3. Firmware and Software Description

3.1.1Starting the Firmware Download

If an instrument is already connected to one of TECAN's programs, close the program or disconnect the instrument from the program.

To start the Firmware Download Software click the RdrDownload icon on the desktop or click the Start button on the lower taskbar and select Programs – TECAN and select Rdr Download. .

The following dialog box is displayed:

Click Connect Instrument… to establish a link from the instrument to the computer.

The Setup Port dialog box appears:

Select <Find any> and click OK.

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3. Firmware and Software Description

3.1.2Open file

To Download the new Firmware version, click Open file... and select the correct *.upd file from the new window. See picture below. Click Download and the new firmware is Downloaded.

Note:

Do NOT switch off the instrument during the Download process.

When the Download process is finished, the right window displays the Download Report and the message, "Firmware Download successfully finished!".

Select Print Report from the <File> menu to print the Download Report.

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3. Firmware and Software Description

3.2 Rdr OLE Server

This program enables the user to define the settings of:

Instrument modes

Filter definition

Measurement modes

3.2.1Installation of Instrument Control Software

For more information about installing the software, see Magellan or XRead Plus manual,

which can be found on the TECAN Reader PC Package CD.

3.3Sunrise Instrument Settings

This program enables the user to define the settings of:

Instrument modes

Filter definition

Measurement modes

3.3.1Installation of SUNRISE Instrument Settings Software

The SUNRISE Instrument Settings software is installed using the following procedure:

•Insert TECAN Reader PC package CD into the required CD ROM drive.

•The Setup dialog box is displayed. Click the Service and Settings button. Click the Setup button for the SUNRISE Instrument Settings. The installation program is started, and the SUNRISE Instrument Settings are installed.

•A series of dialog boxes will appear, read each one, enter any necessary information and click Next to continue.

•The files are then installed and the program icon is created.

•When the Installation Complete dialog box appears, click Finish and the SUNRISE Instrument Settings program is ready to be used.

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3. Firmware and Software Description

3.3.2Starting the SUNRISE Instrument Settings

In case an instrument is already connected to one of TECAN's programs, close the program or disconnect the instrument from the program.

Click the SUNRISE Instrument Settings icon on the desktop, if present, or click the Start button on the lower taskbar and select Programs – TECAN and select

SUNRISE Instrument Settings. The following dialog box is displayed:

Select the correct communication port and the baudrate. Click Next.

3.3.3Define Instrument Mode

The following dialog box is displayed:

To use the instrument with software designed for former TECAN readers, select the appropriate instrument modes and baudrate. Click Next.

Sunrise mode	It is recommended to use the SUNRISE mode with 9600 baud.
Spectra mode	Simulates a SPECTRA Reader.
Rainbow mode	Simulates a Rainbow Reader
ATC mode	Simulates an ATC Reader.

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3. Firmware and Software Description

3.3.4Define Filter

The following dialog box is displayed:

Click the Filter slide Out button to move the filter out of the instrument.

To insert a filter slide, open the filter compartment manually and slide the filter into the slot, so that the filter end of the slide is inserted first. (Do not force the filter slide into the instrument beyond the point of resistance).

Click the Filter slide In button and the filter is inserted.

Pos1 - 4 show the filter values for the currently loaded absorbance filters.

The instrument is able to recognize predefined filter slides and the filter values for these slides must not be changed. However, if the filters in the filter slide have been changed (by a service engineer) or if a new undefined customized filter slide is to be used, the filter slides need to be defined.

To define the filter values for a new filter slide, enter the required wavelengths in the text boxes. Click Next.

The wavelength range for the SUNRISE is 340 - 750 nm.

It is not possible to define the tuneable wavelength filter slide from the

Sunset Instrument Settings

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3. Firmware and Software Description

3.3.5Define Measurement Mode

The following dialog box is displayed:

Select the appropriate measurement mode.

Click Finish and the following dialog box is displayed:

The measurement mode has now been set successfully.

If the filter values for the new filter slide has been defined, then the following dialog box is displayed:

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3. Firmware and Software Description

3.4 SunSet Software (Service tool kit is necessary!)

This program enables the user to adjust the instrument:

Device configuration

Lamp adjust

Plate calibration

Measurement offsets

User configuration

3.4.1Installation of SUNSET Software

The SunSet Software is installed using the following procedure:

•Double click the setup icon to automatically install the SunSet software or click the Start button on the lower taskbar and select <Run…> from the menu and then browse for the correct setup file.

•A series of dialog boxes will appear, read each one, enter any necessary information and click Next to continue.

•The files are then installed and the program icon is created.

•When the Installation Complete dialog box appears, click Finish and SunSet Software is operational.

3.4.2Starting the SunSet Software

•Perform the Sunset test with filterslide G or filterslide A.

•Filterslide G ensures that the entire wavelength range is checked (340 - 750 nm)

•It is not possible to do the adjustments

with the Tuneable Wavelength filterslide.

If an instrument is already connected to one of TECAN's programs, close the program or disconnect the instrument from the program.

To start the SunSet Software click the Start button on the lower taskbar and select Programs and then TECAN –from the menus and select SunSet or click the SunSet icon in the directory C:\Programme\Tecan\SunSet.

The following dialog box is displayed:

Select Connect from the Setup menu or press <Strg> and <F5> together to connect the instrument.

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