Tecan Sunrise Service manual

Technical Manual for

SUNRISE

RC, TW, TS, TC, BC

Document Part No. T 137 302

February 2002

Document Revision: 1.0

Firmware Revision Level: 3.xx

TECAN Affiliates and Service Centers

Austria

Tecan Austria GmbH
Untersbergstrasse 1a
A-5082 Grödig / Salzburg
Austria
Tel.: [phone]
Fax: [phone]

Switzerland
Tecan Schweiz AG
Seestrasse 103
CH-8708 Männedorf
Switzerland
Tel.: + 41 1 922 81 11
Fax. : [phone]

France
Tecan France S.A.
Parc d'Activités de Pissaloup
Batiment Hermes II
Rue Edouard Branly
F-78190 Trappes
France
Tel.: [phone]
Fax: [phone]

Japan
Tecan Japan Co. Ltd
Meiji Seimei Fuchu Building 10F
1-40 Miyamachi
Fuchu City, Tokyo
Japan
Tel.: [phone]
Fax: [phone]

Spain
Tecan Spain
Sabino de Arana, 32
E-08028 Barcelona
Spain
Tel.: [phone]
Fax: [phone]

USA
Tecan Boston
200 Boston Avenue
Suite 3000
Medford, MA 02155
USA
Tel.: [phone]
Fax: [phone]

United Kingdom
Tecan UK
Theale Court
11-13 High Street
Theale
UK-Reading RG7 5AH
United Kingdom
Tel.: [phone]
Fax: [phone]

Asia
Tecan Asia (Pte) Ltd.
80, Marine Parade #13-04
Singapore 449269
Singapore
Tel.: [phone]
Fax: [phone]

Belgium
Tecan Benelux B.V.B.A.
Vaartdijk 55
B-2800 Mechelen
Belgium
Tel.: [phone]
Fax. [phone]

Italy
Tecan Italia S.r.l.
Via F.lli Cervi
Palazzo Bernini
Centro Direzionale Milano2
20090 Segrate (Mi)
Italy
Tel.: [phone]
Fax: [phone]

USA
Tecan US
P.O. Box 13953
Research Triangle Park,
NC 27709
USA
Tel.: [phone]
Fax: [phone]

Germany
Tecan Deutschland GmbH
Theodor-Strom-Straße 17
D-74564 Crailsheim
Germany
Tel.: [phone]
Fax: [phone]

Netherlands
Tecan Benelux B.V.B.A.
Industrieweg 30,
NL-4283 Giessen,
Netherlands;
Tel.: [phone]
Fax: [phone]

2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

WARNING

CAREFULLY READ AND FOLLOW THE INSTRUCTIONS

PROVIDED IN THIS MANUAL BEFORE OPERATING THE

INSTRUMENT.

Notice

Every effort has been made to avoid errors in text and diagrams, however,
TECAN Austria Ges.m.b.H. assumes no responsibility for any errors which may
appear in this publication.

It is the policy of TECAN Austria Ges.m.b.H. to improve products as new
techniques and components become available.TECAN Austria Ges.m.b.H.
therefore reserves the right to change specifications at any time.

We would appreciate any comments on this publication.

TECAN Austria Ges.m.b.H.

Untersbergstraße 1A

A-5082 Grödig/Salzburg

AUSTRIA / EUROPE

Telephone: [phone]

FAX: [phone]

E-mail: [e-mail]

Copyright Information

The contents of this manual are the property of TECAN Austria Ges.m.b.H and
are not to be copied, reproduced or transferred to another person or persons
without our prior written permission.

Copyright  TECAN Austria Ges.m.b.H
All rights reserved.
Printed in Austria.

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3

Warnings, Cautions and Notes

There are three types of informational notices used in this manual. These
notices highlight important information or warn the user of a potentially
dangerous situation. The following notices are:

Note: Gives helpful information.

Caution

Indicates a possibility of instrument damage or data loss if

INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY,

LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS

instructions are not followed.

WARNING

ARE NOT FOLLOWED.

4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

Table of Contents

1. General

1.1 Introduction ...............................................................................1-1

Abbreviations:.............................................................................. 1-1

1.1.1 Available Options for SUNRISE .................................................. 1-2

1.2 Instrument Description ............................................................. 1-3

1.2.1 Remote Control ........................................................................... 1-3

1.2.2 Touchscreen................................................................................ 1-3

1.2.3 Back Panel Connections ............................................................. 1-4

1.3 Filter Carriage Description .......................................................1-5

1.3.1 SUNRISE Standard Filter Carriage ............................................. 1-5

1.3.2 Sunrise Tuneable Wavelength Filter Carriage.............................1-5

1.3.3 Software Features .......................................................................1-6

1.4 Instrument Features.................................................................. 1-6

1.4.1 Measurement Modes................................................................... 1-6

1.4.2 Microplate Shaking...................................................................... 1-7

1.5 Instrument Accessories............................................................ 1-7

Computer Software for Personal Computer ................................1-7

2. Installation Procedure

2.1 Introduction ...............................................................................2-1

2.2 Unpacking and Inspection........................................................ 2-1

2.2.1 Unpacking Procedure.................................................................. 2-2

2.3 Power Requirements................................................................. 2-2

2.4 Environmental Requirements...................................................2-2

2.5 Instrument Installation Procedure ........................................... 2-3

2.5.1 Installation of Instrument Control Software..................................2-3

3. Firmware and Software Description

3.1 Rdr Download Software............................................................ 3-1

3.1.1 Starting the Firmware Download ................................................. 3-2

3.1.2 Open file ......................................................................................3-3

3.2 Rdr OLE Server.......................................................................... 3-4

3.2.1 Installation of Instrument Control Software..................................3-4

3.3 Sunrise Instrument Settings ....................................................3-4

3.3.1 Installation of SUNRISE Instrument Settings Software ............... 3-4

3.3.2 Starting the SUNRISE Instrument Settings ................................. 3-5

3.3.3 Define Instrument Mode ..............................................................3-5

3.3.4 Define Filter .................................................................................3-6

3.3.5 Define Measurement Mode .........................................................3-7

3.4 SunSet Software (Service tool kit is necessary!) ................... 3-8

3.4.1 Installation of SUNSET Software................................................. 3-8

3.4.2 Starting the SunSet Software ...................................................... 3-8

3.4.3 Device configuration.................................................................. 3-10

3.4.4 Mirror and lamp adjustment....................................................... 3-10

3.4.5 Plate calibration......................................................................... 3-11

3.4.6 Measurement offset (Inhouse plate is necessary)..................... 3-12

3.4.7 User configuration ..................................................................... 3-13

3.4.8 SunSet Options Menu ...............................................................3-14

Filter control............................................................................... 3-14

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 5

Plate Movements....................................................................... 3-15

Duration test ..............................................................................3-15

3.5 Sunrise Diagnosis Tool ..........................................................3-16

3.5.1 Installation of Sunrise Diagnosis Tool ....................................... 3-16

3.5.2 Connect instrument ................................................................... 3-17

3.5.3 Get Diagnosis Information......................................................... 3-18

3.5.4 Duration test ..............................................................................3-18

3.5.5 Print report................................................................................. 3-19

3.6 Error messages .......................................................................3-19

4. Removing and Replacing the Instrument Cover

4.1 Removal of Instrument Top Cover........................................... 4-1

4.2 Removal of the Front Cover .....................................................4-3

4.3 Replacing the Front Cover .......................................................4-4

4.4 Replacing the instrument top cover ........................................ 4-4

4.5 Removal of Touchscreen.......................................................... 4-5

5. Optical System

5.1 Introduction ...............................................................................5-1

5.2 Optical System Description...................................................... 5-1

5.3 Optical System Diagram ........................................................... 5-2

SUNRISE ST (Option Standard Optic) Instrument ...................... 5-2

SUNRISE TW (Tuneable Wavelength Optic) Instrument ............ 5-2

5.3.1 Measurements with Wavelengths below 400 nm ........................ 5-3

5.3.2 Agglutination Measurement Mask for SUNRISE ST Option........5-3

5.4 Lamp Replacement ...................................................................5-4

5.5 Filter Slide ..................................................................................5-5

5.5.1 SUNRISE Filter Slide (standard filter slide)................................. 5-5

5.5.2 Replacement of Filters in SUNRISE Filter Slides........................ 5-6

5.5.3 Filter Coding for SUNRISE Filter Slides ...................................... 5-7

5.5.4 Definition of the filter slide codes:................................................ 5-8

Slide D......................................................................................... 5-8

5.6 Filter slide ( Tuneable wavelength).......................................... 5-9

5.7 Lamp and Mirror Unit Parts .................................................... 5-11

5.7.1 Heat Filter and Air Filter ............................................................5-11

5.7.2 Mirror, Optical Lens ...................................................................5-12

For Mirror Replacement: ...........................................................5-12

For Optical Lens Replacement.................................................. 5-13

5.8 Filter Guide (standard instrument ) .......................................5-13

5.9 Filter Guide ( Tuneable Wavelength):.................................... 5-15

5.10 Fiber Bundle ............................................................................5-16

5.10.1 Difference between Standard Fiber Optic and Tuneable Wavelength

Fiber Optic................................................................................. 5-17

5.11 Further Parts belonging to the Optical System.................... 5-17

5.12 Blocking Filter Wheel for Tuneable Wavelength .................. 5-18

5.13 Filter Wheel Properties ...........................................................5-19

5.14 Mirror for Barcode Scanner.................................................... 5-19

6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

6. Mechanical System

6.1 Introduction ...............................................................................6-1

6.2 Transport System Description .................................................6-1

6.3 Measurement Procedure ..........................................................6-1

Inside Shaking............................................................................. 6-2

6.4 The Transport Box Assembly ..................................................6-2

6.5 Exchanging the Transport Box ................................................ 6-3

6.6 Transport Box Adjustments .....................................................6-5

6.7 Replacing the Temperature control unit .................................6-5

7. Electronic System

7.1 Introduction ...............................................................................7-1

7.2 Interconnection diagram (standard RC)..................................7-2

7.3 Voltage diagram (standard RC)................................................ 7-3

7.4 Main Board................................................................................. 7-4

7.5 Replacing the Main Board ........................................................7-5

7.6 Power Board (standard)............................................................ 7-7

7.7 Exchanging the Power Board (standard)................................ 7-8

7.8 Power Board (tuneable wavelength)........................................7-9

7.9 TW Board ................................................................................7-11

7.10 Thermo Board.......................................................................... 7-12

7.11 12 Volt Board ........................................................................... 7-13

7.12 Power Supply board................................................................ 7-14

7.13 Replacing the Power Supply .................................................. 7-15

7.14 Barcode Board......................................................................... 7-16

7.15 Optical Switches...................................................................... 7-17

8. Interfaces

8.1 Computer Interface ...................................................................8-1

8.1.1 Hardware Specifications.............................................................. 8-1

8.1.2 Pin Designation ........................................................................... 8-1

8.1.3 RS-232-C Interface Lines............................................................ 8-2

Requirements for the Serial Interface:......................................... 8-2

Description of the serial Interface:............................................... 8-3

Implemented RS-232-C Interface Lines : .................................... 8-3

Available Setups:......................................................................... 8-3

8.2 Handshakes: ..............................................................................8-4

Software Handshake: ..................................................................8-4

8.3 Commands Response Time: .................................................... 8-4

8.4 Command Syntax for the Serial Interface:.............................. 8-4

Sunrise Mode (Standard): ........................................................... 8-4

Spectra Mode (for compatibility):................................................ 8-4

8.5 Synchronization and Data Format ........................................... 8-5

8.6 Signal Levels .............................................................................8-5

8.7 Software Specifications............................................................ 8-6

8.8 Handshake ................................................................................. 8-6

8.9 Timing Of Messages .................................................................8-6

8.10 Communication Messages ....................................................... 8-6

8.11 Printer Interface......................................................................... 8-7

8.11.1 Character Set Setting ..................................................................8-7

8.11.2 Hardware Specifications.............................................................. 8-7

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 7

8.11.3 Connector.................................................................................... 8-7

8.12 Parallel Interface Lines ............................................................. 8-8

8.13 Table of Connections................................................................ 8-8

8.14 Key to Table ...............................................................................8-9

Connector Pin Signals................................................................. 8-9

Strobe.......................................................................................... 8-9

Data 1 - Data 8 ............................................................................8-9

Acklng - Acknowledge .................................................................8-9

Busy ............................................................................................8-9

8.15 Connection with Touchscreen interface connector.............8-10

8.15.1 XChange Files:.......................................................................... 8-10

9. Sunrise Service Tool Kits for Sunrise RC and Options

9.1 Service tool kit 1 (S 039 341) .................................................... 9-1

9.1.1 Introduction.................................................................................. 9-1

9.2 Installation Procedure............................................................... 9-2

9.3 Sunrise Diagnosis Software..................................................... 9-2

9.3.1 Explanation of the Output of the Sunrise Diagnosis Tool........... 9-3

9.4 Sunrise SUNSET Software .......................................................9-6

9.5 QC Pac 2 Service software:...................................................... 9-7

9.6 Flashcard Download (S039351) ...............................................9-8

9.7 Flashcard for Touchscreen unit............................................... 9-8

9.8 Barcode Upgrade Kit................................................................. 9-9

9.8.1 General........................................................................................ 9-9

9.8.2 Installation ................................................................................. 9-10

9.8.3 Hardware Installation................................................................. 9-12

9.8.4 Software Installation and Adjustment ........................................9-15

9.9 Overview of Single commands: ............................................. 9-22

10. Maintenance

10.1 Instrument Disinfection ..........................................................10-1

10.2 Disinfection Solutions ............................................................10-1

10.3 Disinfection Procedure ........................................................... 10-2

10.4 Disinfection Certificate ........................................................... 10-2

10.5 Preventative Maintenance Plan for SUNRISE Instruments .10-3

10.5.1 Daily ..........................................................................................10-3

10.5.2 Weekly....................................................................................... 10-3

10.5.3 Every Six Months ......................................................................10-3

10.5.4 Yearly (Service Technician Required)....................................... 10-3

10.5.5 Every Four Years ......................................................................10-3

10.6 Electrostatic Discharge Information...................................... 10-4

10.6.1 Generating Static....................................................................... 10-4

10.6.2 Preventing Electrostatic Damage to Equipment........................ 10-5

10.6.3 Personal Grounding Methods.................................................... 10-5

8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

11. Performance Testing / Quality Control

11.1 Introduction .............................................................................11-1

11.2 Operating for Maximum Performance ................................... 11-1

11.2.1 Instrument Location................................................................... 11-1

11.2.2 Operating Procedure .................................................................11-2

11.2.3 Self Check Procedure ...............................................................11-2

11.3 Performance Tests .................................................................. 11-2

11.3.1 QC PAC 2.................................................................................. 11-3

11.3.2 Microplate Test.......................................................................... 11-3

11.3.3 High Meniscus Liquids ..............................................................11-4

Agglutination Method................................................................. 11-5

Manual Method.......................................................................... 11-6

Example ....................................................................................11-6

11.4 Quality Control Testing........................................................... 11-7

11.4.1 Precision Testing....................................................................... 11-7

11.4.2 Instrument Accuracy.................................................................. 11-8

11.4.3 Instrument Linearity................................................................... 11-9

12. Trouble Shooting

12.1 Introduction .............................................................................12-1

13. Parts for Sunrise

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 9

1. General

1. General

1.1 Introduction

SUNRISE Remote Control is intended for use with only external software.

Sunrise with TS Option is intended for use with external software

and in Stand alone mode.

The SUNRISE instruments are fully automatic, microprocessor controlled readers
designed for professional use, enabling the user to measure the light absorbency
(optical density) of samples in 96 well microplates according to the specifications
described in this manual.

Abbreviations:

RC Remote control
TW Tuneable wavelength option
TS Touchscreen option
TC Temperature control option
BC Barcode option

Results obtained using the SUNRISE are influenced by the proper use of

the instrument, according to the instructions given in this manual, as well

as the liquid compounds used (reagents, chemistry). The instructions for

use, storage and other manipulations in connection with samples or

reagents have to be strictly followed. Taking this fact into consideration,

results must be interpreted carefully.

By reading twelve wells simultaneously, the instrument is able to measure a
microplate in approximately eight seconds using the dual wavelength method.

With an innovative range of options, this versatile MTP reader gives diagnostic
and research laboratories all the features for numerous purposes.

Based on a new design concept EPAC, the excellent optical performance and
high quality of the SUNRISE will guarantee fast, reproducible and accurate
measurements.

The SUNRISE is designed to be fitted into TECAN robotic systems.

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-1

1. General

1.1.1 Available Options for SUNRISE

The Sunrise is a modular system, so you can create your own tailor-made
instrument that meets exactly your needs. Options such as a touch screen
combined with a WindowsCE based on-board software, free wavelength
selection, temperature control and a bar code scanner can be added onto the
basic system which is dedicated to remote controlled computer operation.

Touchscreen

B037302

SUNRISE
Remote Controlled

Tuneable wavelength
from 400 – 700 nm

B037306

Temperature control

B037303

Barcode scanner

B037304

CAUTION

IF THE INSTRUCTIONS GIVEN IN THIS MANUAL ARE NOT CORRECTLY

CARRIED OUT, THE INSTRUMENT MAY EITHER BECOME DAMAGED OR

MAY NO LONGER BE ABLE TO PERFORM ITS PROCEDURES

CORRECTLY AND THE ACCURACY OF THE INSTRUMENT

CAN NO LONGER BE GUARANTEED.

For more information about the operating instructions, see Magellan

or XRead Plus manuals.

The available options are Retro-fit by TECAN Austria only.*

FOLLOW LASER SPECIFICATIONS (see 1.3 back panel connections)

*Except Barcode Upgrade Kit S 039 386

Only by TECAN certified FSE

1-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

1. General

1.2 Instrument Description

The illustrations below show the components of the instruments.

1.2.1 Remote Control

Power ON LED

Plate Support

1.2.2 Touchscreen

Lamp Compartment

Cartridge and Filter Block Compartment

Touchscreen

Sunrise
Memo Card

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-3

1. General

1.2.3 Back Panel Connections

The illustration below shows the connections located in the back panel of the
instrument.

BarCode Laser Scanner labels
( only with the barc ode option )

TECAN

A-5082 AUSTRIA

VA

50 / 60

Hz

MADE IN
AUSTRIA

Instruments Main Fuses

CAUTION

LASER RADIATION -

DO NOT STARE INTO BEAM

CLASS II LASER PRODUCT

Complies with 21CFR

and 1040.10 and 104 0.11

LASERSTRAHLUNG

NICHT IN DEN STRAHL BLICKEN

LASER KLASSE 2

Leistung: <1mW

Wellenlänge: 675nm

EN60825-1: 1994+A11:1 996

TECAN

TYPE

Sunrise

ART.-NR.

F0 3 9 3 x x 11 0

SER.-NR.

xxxxx

VOLTAGE

AC 100 - 120 / 220 - 240

M-CODE

xxxxxx

ON / OFF Switch

Mains Socket

I

0

LISTED

U

U

L

R

LABORATORY EQUIPMENT

L

R

C

8C64

2 x F2A

TECAN

Printer Connector
( Only funct ions with
the touch screen option )

RS 232

PRINTER

Serial RS 232 Connector

All connected devices must be approved and listed as per EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data Processing Devices

1-4 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

1. General

1.3 Filter Carriage Description

The SUNRISE instrument can use the following types of filter carriages:

SUNRISE Standard and SUNRISE Gradient Filter (with tuneable wavelength
option).

1.3.1 SUNRISE Standard Filter Carriage

The SUNRISE standard filter carriage is fitted with up to four narrow band
interference filters which have a fixed wavelength.

SUNRISE Standard Filter Carriage

When a wavelength is selected, the entered wavelength is compared against the
list of entered filter values for this filter carriage.

If the required filter is fitted in the filter carriage, the filter carriage is moved so that
the required filter is in the light beam.

For more information about the definition of the new and customized filter

slides, see 3.8 Define Filter.

1.3.2 Sunrise Tuneable Wavelength Filter Carriage

• Over 300 wavelengths available from one Gradient filter slide.

• Complete Spectrum scanning - useful for screening new or unknown

compounds

• UV light range available on standard filter slide

• High flexibility in one instrument

SUNRISE TW functions only with the Blocking Filter Wheel

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-5

See Chapter 5.12 Blocking Filter Wheel for Tuneable Wavelength.

1. General

1.3.3 Software Features

SUNRISE Remote Control is intended for use with only external software.

Sunrise Touchscreen is a stand alone device, that works with windows CE.

For more information about the software features, see the appropriate

individual manuals. For example: refer to the Magellan Reference manual.

1.4 Instrument Features

Microplates can be measured using the following features:

• Various measurement modes

• Single or dual wavelength measurements

• Microplate shaking

• Temperature controlled Incubation

1.4.1 Measurement Modes

The instrument can be set to use the following measurement modes:

Fast Plate transport is moved quickly under the measurement diodes so

that a fast measurement is obtained. (Default setting).

Accurate Plate transport is moved slowly under the measurement diodes so

that a very accurate measurement is obtained.

Center This option measures the optical density only at the center of the

well.

With the Fast and Accurate measurement modes, the optical density is measured
at three positions across the wells and the average measured optical density
value from the three measurements is used as the optical density of the well.

The accurate measurement cycle should always be used when measuring

high optical densities.

The Center measurement mode should be used if the liquid in the microplate
produces a high meniscus, as an incorrect optical density could be obtained if the
optical density is measured at three positions. If an Agglutination measurement is
performed, all the measurement positions are used.

For more information about setting the measurement mode,

1-6 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

see 3.9 Defining the SUNRISE Instrument Settings.

1. General

1.4.2 Microplate Shaking

The SUNRISE is able to shake the microplate before it is measured. Use external
software (for example: Magellan) to set the shaking modes.

The microplate can also be shaken between each of the kinetic measurement
cycles.

When using a 96 well plate, spillage may occur if the wells are filled with more
than 300µl, while using high shaking mode.

1.5 Instrument Accessories

The table below contains the order numbers for instrument accessories:

Part Name Part Order Number

Halogen lamp................................................................................. 3 709 008

Reader to external computer cable................................................ 3 350 005

QC Pac 2 for SUNRISE and SPECTRA ........................................ B 037 358

Additional filter slide .......................................................................B 036 301

Memory card .............................................................................B 037 359 01

Flash card for Magellan Windows CE (English) ............................S 039 351

TS - Pen ......................................................................................... B 037 360

Barcode Upgrade Kit...................................................................... S 039 386

Sunrise Service tool kit ..................................................................S 039 341

Computer Software for Personal Computer

Related Software

Part of Reader PC Package:

Software Functionality

Magellan

XRead Plus

SUNRISE instrument setting

RDR Download

SUNRISE Error Diagnosis

Sunset Software

Instrument control and data reduction

Instrument control and transfer of raw data to
Excel.

Enables settings of SUNRISE instrument
(SUNRISE, SPECTRA, ATC mode and so on).

Enables Download of new firmware from PC to
reader.

Creates printout of instrument status for service
purposes.

For servicing the sunrise and performing all
instrument adjustments
(only for trained/ certified persons!)

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 1-7

2. Installation Procedure

2. Installation Procedure

2.1 Introduction

This chapter contains the necessary information for installing the instrument.

The installation procedures involve unpacking, environmental requirements,
power requirements and interfacing.

2.2 Unpacking and Inspection

The delivered instrument is shipped in one carton, which includes:

Power cable

Computer connection cable

SUNRISE Operating manual, XRead Plus manual

Spare fuses

A software CD, which also contains the XRead Plus program and Magellan

Foam Packaging

demo program (30 day working license).

Accessory box

Foam

Packaging

Instrument

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 2-1

2. Installation Procedure

2.2.1 Unpacking Procedure

1. Visually inspect the container for damage, before opening it.

Report any damage immediately.

2. Place the carton in an upright position and open it.

The Cartridge and Filter Block Compartment is fixed with adhesive tape.

3. Lift the instrument out of the carton and place it on a flat surface, free from
dust, vibration and away from direct sunlight.

4. Visually inspect the instrument for loose, bent or broken parts.

Report any damage immediately.

5. Compare the instrument's serial number, attached on the rear panel of the
instrument, against the serial number of the instrument, on the delivery
(shipping) note.

6. Check the instrument accessories against the delivery (shipping) note.

7. Open the plate support area cover and remove the foam strip that is used as
the microplate transport lock.

8. Please save all packing materials, as it may be required for later
transportation.

2.3 Power Requirements

The instrument is auto sensing for the supplied voltage, and therefore does not
have to be set for the correct voltage.

Connect the instrument only to a electricity supply system with protective earth.

WARNING

TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE

REPLACED WITH THE SAME TYPE AND RATING OF FUSES.

2.4 Environmental Requirements

The instrument should be placed on a flat, level surface that is free from dust,
solvents and acidic vapors.

Vibration and direct sunlight must be avoided, to ensure correct results.

2-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

2. Installation Procedure

2.5 Instrument Installation Procedure

The following procedures detail the necessary steps to be followed when
installing the instrument.

WARNING

BEFORE THE INSTRUMENT IS INSTALLED AND SWITCHED ON, IT SHOULD

BE LEFT TO STAND FOR AT LEAST THREE HOURS, SO THERE IS NO

POSSIBILITY OF CONDENSATION CAUSING A SHORT CIRCUIT.

When the requirements above have been met, installation is carried out using the
following procedure:

1. Place the instrument into the required position.

Ensure that the distance between the back panel of the instrument and the
wall, is at least 10 cm.

2. Connect the instrument to the external computer with the required interfacing
cable.

The interfacing cable is connected into the 9 pin serial interface socket, in
the back panel.

3. Ensure that the mains power switch in the back panel of the instrument is in
the off position.

4. Insert the power cable into the mains power socket in the back panel.

5. Switch the instrument on using the mains power switch in the back panel.

The instrument is now ready to measure microplates.

2.5.1 Installation of Instrument Control Software

For more information about installing the software, see Magellan or XRead

Plus manual, which can be found on the TECAN Reader PC Package CD.

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3. Firmware and Software Description

3. Firmware and Software
Description

3.1 Rdr Download Software

This program enables the user to Download a new Firmware:

The Rdr Download software is installed using the following procedure:

• Insert TECAN Reader PC package CD into the required CD ROM drive.

• The Main menu is displayed. Click the Software button. Click the Setup

button for the Firmware Download. The installation program is started.

• A series of dialog boxes will appear, read each one, enter any necessary
information and click Next to continue. The files are then installed and the
program icon is created.

• When the Installation Complete dialog box appears, click Finish and the
Firmware Download

program is ready to be used.

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3. Firmware and Software Description

3.1.1 Starting the Firmware Download

If an instrument is already connected to one of TECAN's programs, close the
program or disconnect the instrument from the program.

To start the Firmware Download Software click the RdrDownload icon on the
desktop or click the Start button on the lower taskbar and select Programs –
TECAN and select Rdr Download. .

The following dialog box is displayed:

Click Connect Instrument… to establish a link from the instrument to the
computer.

The Setup Port dialog box appears:

Select <Find any> and click OK.

3-2 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002

3. Firmware and Software Description

3.1.2 Open file

To Download the new Firmware version, click Open file... and select the correct
*.upd file from the new window. See picture below. Click Download and the new
firmware is Downloaded.

Note:

Do NOT switch off the instrument during the Download process.

When the Download process is finished, the right window displays the Download
Report and the message, "Firmware Download successfully finished!".

Select Print Report from the <File> menu to print the Download Report.

February 2002 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 3-3

3. Firmware and Software Description

3.2 Rdr OLE Server

This program enables the user to define the settings of:

Instrument modes

Filter definition

Measurement modes

3.2.1 Installation of Instrument Control Software

For more information about installing the software,

which can be found on the TECAN Reader PC Package CD.

see Magellan or XRead Plus manual,

3.3 Sunrise Instrument Settings

This program enables the user to define the settings of:

Instrument modes

Filter definition

Measurement modes

3.3.1 Installation of SUNRISE Instrument Settings Software

The SUNRISE Instrument Settings software is installed using the following
procedure:

• Insert TECAN Reader PC package CD into the required CD ROM drive.

• The Setup dialog box is displayed. Click the Service and Settings button.

Click the Setup button for the SUNRISE Instrument Settings. The installation
program is started, and the SUNRISE Instrument Settings are installed.

• A series of dialog boxes will appear, read each one, enter any necessary
information and click Next to continue.

• The files are then installed and the program icon is created.

• When the Installation Complete dialog box appears, click Finish and the

SUNRISE

Instrument Settings program is ready to be used.

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3. Firmware and Software Description

3.3.2 Starting the SUNRISE Instrument Settings

In case an instrument is already connected to one of TECAN's programs, close
the program or disconnect the instrument from the program.

Click the SUNRISE Instrument Settings icon on the desktop, if present, or click
the Start button on the lower taskbar and select Programs – TECAN and select
SUNRISE Instrument Settings.

The following dialog box is displayed:

Select the correct communication port and the baudrate. Click Next.

3.3.3 Define Instrument Mode

The following dialog box is displayed:

To use the instrument with software designed for former TECAN readers, select
the appropriate instrument modes and baudrate. Click Next.

Sunrise mode It is recommended to use the SUNRISE mode with 9600 baud.

Spectra mode Simulates a SPECTRA Reader.

Rainbow mode Simulates a Rainbow Reader

ATC mode Simulates an ATC Reader.

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3. Firmware and Software Description

3.3.4 Define Filter

The following dialog box is displayed:

Click the Filter slide Out button to move the filter out of the instrument.

To insert a filter slide, open the filter compartment manually and slide the filter
into the slot, so that the filter end of the slide is inserted first. (Do not force the
filter slide into the instrument beyond the point of resistance).

Click the Filter slide In button and the filter is inserted.

Pos1 - 4 show the filter values for the currently loaded absorbance filters.

The instrument is able to recognize predefined filter slides and the filter

values for these slides must not be changed. However, if the filters in the

filter slide have been changed (by a service engineer) or if a new

undefined customized filter slide is to be used, the filter slides need to be

defined.

To define the filter values for a new filter slide, enter the required wavelengths in
the text boxes. Click Next.

The wavelength range for the SUNRISE is 340 - 750 nm.

It is not possible to define the tuneable wavelength filter slide from the

Sunset Instrument Settings

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3. Firmware and Software Description

3.3.5 Define Measurement Mode

The following dialog box is displayed:

Select the appropriate measurement mode.

Click Finish and the following dialog box is displayed:

The measurement mode has now been set successfully.

If the filter values for the new filter slide has been defined, then the following
dialog box is displayed:

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3. Firmware and Software Description

3.4 SunSet Software (Service tool kit is necessary!)

This program enables the user to adjust the instrument:

Device configuration

Lamp adjust

Plate calibration

Measurement offsets

User configuration

3.4.1 Installation of SUNSET Software

The SunSet Software is installed using the following procedure:

• Double click the setup icon to automatically install the SunSet software or
click the Start button on the lower taskbar and select <Run…> from the
menu and then browse for the correct setup file.

• A series of dialog boxes will appear, read each one, enter any necessary
information and click Next to continue.

• The files are then installed and the program icon is created.

• When the Installation Complete dialog box appears, click Finish and

SunSet Software is operational.

3.4.2 Starting the SunSet Software

• Perform the Sunset test with filterslide G or filterslide A.

• Filterslide G ensures that the entire

wavelength range is checked (340 - 750 nm)

• It is not possible to do the adjustments
with the Tuneable Wavelength filterslide.

If an instrument is already connected to one of TECAN's programs, close the
program or disconnect the instrument from the program.

To start the SunSet Software click the Start button on the lower taskbar and
select Programs and then TECAN –from the menus and select SunSet or click
the SunSet icon in the directory C:\Programme\Tecan\SunSet.

The following dialog box is displayed:

Select Connect from the Setup menu or press <Strg> and <F5> together to
connect the instrument.

3-8 Technical Manual for SUNRISE RC, TW, TS, TC, BC No: T 137 302 Rev No: 1.0 February 2002