Sysmex XE Service manual

SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM........................................................................................................................1

2.2 USE OF PRESSURE AND VACUUM .................................. ... ............................................ ................2

2.3 HYDRAULIC SYSTEM........................................................................................................................3

2.4 ANALYSIS PARAMETER....................................................................................................................4

2.5 REAGENT SYSTEM ...........................................................................................................................6

2.6 ANALYSIS FLOW................................................................................................................................7

2.7 FCM DETECTOR BLOCK...................................................................................................................8

2.7.1 Function.............................................................................................................................9

2.8 OPTICAL SYSTEM ...........................................................................................................................11

2.9 HYDRAULIC SEQUENCE DESCRIPTION.......................................................................................12

2.10 HYDRAULIC PARTS............................................................. .. .......................................... ..............33

2.10.1 Solenoid Valve Function ................................................................................................33

2.10.2 HYDRAULIC PARTS LOCATION .................................................... ..............................35

2.11 SRV STRUCTURE.............................................................................................. ... .........................40

2.12 CONVERSION TABLE OF VACUUM AND PRESSURE VALUES..................................................42

2.13 Hydraulic System Difference Between XE-2100 and XE-2100 DC ........................................44

2.13.1 XE-2100 Hydraulic System............................................................................................44

2.13.2 XE-2100DC (XE-2100C) Hydraulic System...................................................................44

February 5, 2009XE-series S/M

SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM

The system to provide the required pressure and vacuum for main unit operation consists of the following. Pressurized air is regulated at 2.5 kg/cm water droplets are removed by an air filter. An air drier is used to remove any remaining moisture before it is supplied to the main unit. The pressure of the dry air is regulated at 1.6, 0.7, and 0.3 kg/cm

The vacuum (400 mmHg or higher) is adjusted to 300 mmHg by the bellows unit in the main unit. A filter is provided on the bellows unit to prevent the needle in the bello w from accumulating dirt.

The outline of pneumatic control system is shown below.

by a relief valve in the pneumatic unit, any dust and

2-1 February 5, 2009XE-series S/M

2.2 USE OF PRESSURE AND VACUUM

Use of pressure and vacuum for the main unit are as follows.

Type Name Functions

Pressure 2.5 kg/cm

1.6 kg/cm

0.7 kg/cm

0.3 kg/cm

Vacuum Source Vacuum Aspiration of reagent and driving diaphragm pumps.

300 mmHg Aspiration of waste to WC1

2 2 2

Driving master valves, air cylinders, and diaphragm pump (DP 5mL for FCM (EPK) replenishment)

Sheath Flow forming for FCM Draining waste chamber and driving of diaphragm pumps Sheath Flow forming for RBC

2-2 February 5, 2009XE-series S/M

2.3 HYDRAULIC SYSTEM

WBC/RBC Block Diagram

RBC/HGB Block Diagram

2-3 February 5, 2009XE-series S/M

2.4 ANALYSIS PARAMETER

Analysis Block

Principle Analysis

Channel

Information

Parameter

Analytical

Parameter

Reagent

Analysis Block

Principle Analysis

Channel

Information

Parameter

RBC/PLT HGB IMI

Searth Flow DC Detection Method Colorimetric RF/DC

RBC/PLT Channel HGB Channel IMI Channel

PLT Histogram RBC

Histogram

PLT HCT RBC HGB -

MCV,MCH,MCHC,

MPV,PDW, P-

LCR

CELL P ACKII / SE SEATHII SULFOLYSER STOMATOLYSER-IM

WBC/BASO

Channel

WBC/BASO

Scattergram

WBC# BASO

BASO

RDW-SD/CV

WBC/RET

Flowcytometry by using Semi-Conductor Laser

4DIFF Channel NRBC

Lymph

4DIFF Scattergram

MONO

NEUT

EOSI

IMI Scattergram

Channel

NRBC

Scattergra

NRBC%

NRBC#

RET

Channel

RET

Scattergra

RET%

RET# PLT-

Analytical

Parameter

Reagent

STROMATOLYS

ER-FB

STROMATOLYSER-4D

Lysing solution/ Staining Dye (NEW)

STROMAT

OL YSER-

NR Lysing

solution/

Staining

Dye (NEW)

L/M/HFR,

IRF

RET

SERACHII

Diluent

solution/

Staining

Dye (NEW)

2-4 February 5, 2009XE-series S/M

*1 4DIFF Scattergram *2 WBC/BASO Scattergram

*3 NRBC Scattergram *4 RET Scattergram

*5 IMI Scattergram

Figure 2-1 Scattergrams

2-5 February 5, 2009XE-series S/M

2.5 REAGENT SYSTEM

Figure 2-2 Reaction Chamber Construction

2-6 February 5, 2009XE-series S/M

2.6 ANALYSIS FLOW

2-7 February 5, 2009XE-series S/M

2.7 FCM DETECTOR BLOCK

NOTE:

Laser Diode is very sensitive to static electricity. Follow the instruction below when you replace the Laser Diode.

- Remove jumper pin from the terminal on the Laser Diode AFTER connecting to PCB No. 2140. If the jumper pin is removed before connecting to PCB No.2140, Laser Diode will be damaged.

- Do not disconnect the connector to the PCB No.2140.

In the worst case, the device inside the Lase r Diode will be destroyed.

The life of the Laser Diode is approx. 10,000 hours.

2-8 February 5, 2009XE-series S/M

2.7.1 Function

(1) Outline

Laser diode apply to samples in sheath flow generated flow cell. FCM Detector Block detects front scattered light, side scattered light, and side fluorescence light from these samples. XE-2100 generates two-dimensional scattergrams from these signals as follows.

X-axis Y-axis WBC# / BASO SSCL FSCP DIFF SSCL SFLP NRBC SFLL FSCP RET SFLP FSCP PLT-O SFLP FSCL

SSCL: Side SCattered peak Logarithm FSCL: Front SCattered peak Logarithm SFLP: Side Fluorescence Light Peak linear SFLL: Side Fluorescence Light peak Logarithm FSCP: Front SCattered Peak linear

Following table shows which receiver is used for each parameter.

FSC Photo diode Side Scattered PMT Side Fluorescence PMT WBC# / BASO Used Used Not Used DIFF Used but not

displayed in

Scattergram NRBC Used Not Used Used RET Used Not Used Used

Used Used

(2) Beam spot generating system

Beam spot generating system consists of laser diode, collimator lens. Laser beam generated b y laser diode is converted to parallel beam by collimator lens. This beam is collected by condenser lens and applies to samples. Beam shape is as follows.

(3) Sheath flow system

Sheath flow is generated in the flow cell. Sample flows center of the flow cell.

(4) Front scattered light system

Front scattered light system consists of beam stopper and FSC condenser lens. Beam stopper shade direct light from the laser diod e. FSC condenser lens collect the scattered light of the sample.

(5) Dichroic mirror

Light below 640 nm wave length reflects to the side scattered light PMT.

2-9 February 5, 2009XE-series S/M

(6) Side fluorescence light system

Side fluorescence light sy st em co nsis t s of int erference filter, PMT (Photo Multiplier Tube). The light through dichroic mirror is filtered by interferenc e filter and fluorescence light over 660 nm wave length is detected by PMT. Detected fluorescence light signal is amplified by pre-amplifier and transmitted to main amplifier.

(7) Side Scattered light syst em

Side Scattered light system consists of ND filter (Neutral Density filter) and PMT. The light through dichroic mirror is attenuated by ND filter and sca ttered light is detected by PMT. Detected scattered light signal is amplified by pre-amplifier and transmitted to main amplifier.

(8) Temperature Control

FCM Detector block is heater controlled to maintain the optical stability.

(9) Cover switch

Cover switch is installed to prevent interference from the outer light and leakage of the laser beam. If the cover is opened, XE-2100 stops laser radiation and massage is displayed.

2-10 February 5, 2009XE-series S/M

2.8 OPTICAL SYSTEM

2-11 February 5, 2009XE-2100 S/M

2.9 HYDRAULIC SEQUENCE DESCRIPTION

0- 5 sec [WB aspiration, reagent chamber]

- SRV turns activating SV60 f or 0 - 0. 5 sec.

- Whole blood pump descend to aspirate diluent for 0 - 2.0 sec.

- SRV is rinsed (perimeter cleaning) by activating SV60 for 0 - 0.5.sec.

- WC2 is drained by activating SV 43 for 1.5 - 7.5 sec.

- WC3 is drained by activating SV 45 for 0.5 - 2.0 sec.

- SNR is replenished by activating SV8 for 0 - 3.0 sec.

[HGB]

- Solution in HGB flow cell is drained by activating SV36 for 3.0 - 3.8 sec.

- HGB Diluent DP dispense diluent into HGB flow cell for rinsing for 3.8 - 4.9 sec.

- SV 11 is activated to push the solution in the air mixing tubing to WC1.

2-12 February 5, 2009XE-2100 S/M

2-13 February 5, 2009XE-2100 S/M

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