SYSMEX CyFlow Cube 8 Operating Manual
CyFlow® Cube 8
Operating Manual
For Research Use Only. Not for use in diagnostic procedures.
CyFlow® Software Version 1.0 RUO
Doc. No.: CY-S-3068RIFUEN | Doc. Rev
.: 032 | 17-05-2018 | EN | CN 383
Imprint
Original Operating Manual
Sysmex Partec GmbH
Am Flugplatz 13
D-02828 Görlitz Germany
www.sysmex-partec.com
All rights reserved. No part of this manual may be reproduced, transcribed or translated in any form
or by any means electronic or otherwise, without the prior permission of Sysmex Partec GmbH.
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Table of contents
1 Introduction .............................................................................................6
1.1 About this operating manual .................................................... 6
1.2 Typographical conventions ...................................................... 7
1.3 General conditions ....................................................................8
1.4 CE compliance ........................................................................... 8
2 Safety ....................................................................................................... 9
2.1 Intended use...............................................................................9
2.2 Prohibited use ............................................................................9
Table of contents
2.3 Reasonably foreseeable misuse ..............................................9
2.4 User definition..........................................................................10
2.4.1 User qualification groups ...........................................................10
2.4.2 User qualification table...............................................................11
2.5 Environmental conditions.......................................................11
2.6 Prohibition and warning signs on the device ....................... 12
2.7 Personal protective equipment .............................................. 12
2.8 Laser safety .............................................................................. 13
2.9 Electrical safety........................................................................13
2.10 Alterations to the device ......................................................... 13
3 Overview ................................................................................................ 14
3.1 Components of the device ......................................................17
3.2 Type plate ................................................................................. 18
3.3 Scope of delivery ..................................................................... 19
3.4 Accessories and spare parts .................................................. 21
4 Transport and storage..........................................................................22
5 Installation .............................................................................................23
5.1 Positioning the device.............................................................23
5.2 Installation and uninstallation ................................................ 23
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Table of contents
6 Operation ...............................................................................................24
6.1 Inserting the Software dongle.................................................24
6.2 Start-up procedure...................................................................24
6.2.1 Switching on the device .............................................................24
6.2.2 Logging in and logging out.........................................................25
6.2.3 Performing a Prime ....................................................................26
6.3 Performing a measurement.....................................................27
6.4 Performing an intermediate cleaning.....................................28
6.5 Shutdown procedure ...............................................................29
6.5.1 Cleaning for shutdown ...............................................................29
6.5.2 Switching off the device .............................................................29
6.6 Software operation...................................................................30
6.6.1 Preparing measurements using the Worklist .............................30
6.6.2 Analysing data and creating reports using the Playlist...............37
7 Troubleshooting....................................................................................39
7.1 Performing a de-bubble procedure ........................................39
7.2 Performing a de-clog procedure.............................................39
7.3 Sheath fluid, waste and fluids.................................................40
7.4 Calibration and Count Check Beads (low, medium, high) ...41
7.5 Measurements and data acquisition ......................................41
8 Cleaning.................................................................................................42
8.1 Cleaning the device .................................................................42
8.2 Performing a cleaning procedure...........................................43
9 Maintenance ..........................................................................................44
10 Disposal .................................................................................................45
11 Technical data .......................................................................................46
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Table of contents
12 Software description ............................................................................50
12.1 Overview ...................................................................................50
12.2 Views of the software window ................................................ 52
12.3 Quick Access Toolbar ............................................................. 56
12.4 Ribbon.......................................................................................58
12.4.1 File tab .......................................................................................58
12.4.2 Home tab ...................................................................................65
12.4.3 Analyze tab ................................................................................ 68
12.4.4 Preview tab ................................................................................ 74
12.4.5 Parameters tab ..........................................................................76
12.4.6 Compensation tab......................................................................84
12.4.7 Statistics tab...............................................................................92
12.4.8 Worklist tab ................................................................................ 97
12.4.9 Playlist tab................................................................................100
12.4.10 Cytometer tab ..........................................................................105
12.4.11 Overlay tab...............................................................................109
12.4.12 Report tab ................................................................................114
12.4.13 Views tab .................................................................................123
12.4.14 Plot Format tab ........................................................................124
12.4.15 Region Format tab ...................................................................128
12.4.16 Overlay Format tab .................................................................. 131
12.5 Panes ......................................................................................136
12.5.1 FCS File Information pane....................................................... 137
12.5.2 Results Hierarchy pane............................................................138
12.5.3 Settings pane........................................................................... 139
12.5.4 Acquire pane............................................................................140
12.5.5 Setup pane...............................................................................143
12.5.6 Stops pane...............................................................................144
12.6 Worklist pane .........................................................................146
12.7 Playlist pane ........................................................................... 154
12.8 Workspace..............................................................................160
12.8.1 Plots tab of the Workspace...................................................... 160
12.8.2 Results tab of the Workspace.................................................. 171
12.8.3 Overlays tab of the Workspace................................................174
12.8.4 Reports tab of the Workspace .................................................179
12.9 Status bar ...............................................................................184
12.10 CyFlow Options......................................................................187
Index.....................................................................................................195
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 5
1Introduction
1 Introduction
1.1 About this operating manual
This operating manual will help you to operate your CyFlow® Cube device.
Emphasis is placed on the installation, start-up and safe operation of the
device.
The basic principles of flow cytometry can be found in the medical literature
(e.g. Howard M. Shapiro, Practical Flow Cytometry, Wiley 2003).
This operating manual comes with the device. Read this operating manual
thoroughly before using the device. Please keep this operating manual for
future reference.
This operating manual is divided in two parts. The first part, chapter 1–11,
gives the user all necessary information for safe handling, scope of delivery,
to set up the device and how to operate the device properly. The general use
of the software and the typical workflows are described in chapter 6.5. This
chapter also is suitable to get to know the functioning of your device.
In the second part, chapter 12, you will find a description of all software
functions and a detailed description of the user interface. This chapter is for
reference.
There are product data sheets for the reagent kits and application notes
available. Please also refer to these documents.
If you have any questions about the content of this operating manual or the
use of the device, contact your local Sysmex representative.
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1.2 Typographical conventions
In this operating manual, warnings are indicated by a signal word. Hazards
are categorised into hazard levels with consequences of differing severity.
Type of hazard
This will result in death or have a serious impact on health.
Information about avoiding the hazard.
1Introduction
Type of hazard
This could result in death or have a serious impact on health.
Information about avoiding the hazard.
Type of hazard
This may have a minor impact on health.
Information about avoiding the hazard.
Type of hazard
This could result in material damage.
Information about avoiding the hazard.
All warnings are structured according to the same model. The type of hazard
is stated after the signal word. Then the consequences that may result from
the hazardous situation are described. And finally, how the hazardous
situation can be avoided.
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1Introduction
1.3 General conditions
Ensure that the following conditions are met when working with the device:
This operating manual must be read prior to using the device.
Report any recurring faults or problems to your local Sysmex
Sysmex Partec GmbH does not accept liability for any damage or personal
injury arising from:
Modifications or manipulation of the device;
Improper use;
Use of non-approved accessories;
Improper handling;
Non-compliance with this operating manual.
representative.
1.4 CE compliance
The device is marked with a CE mark of conformity, which confirms the
compliance with the essential requirements of the following European
Directives:
2014/35/EC on electrical equipment designed for use within certain
voltage limits;
2014/30/EC on electromagnetic compatibility;
2011/65/EC on the restriction of the use of certain hazardous substances
in electrical and electronic equipment.
1.5
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2 Safety
2.1 Intended use
2 Safety
For Research Use Only. Not for use in diagnostic procedures.
The device is a flow cytometer, it offers automation for routine use and
flexibility for research use in the field of flow cytometric applications.
The applications cover:
Multi-colour analysis of immune cells for research purposes;
Leukocyte counting/rare event analysis for research purposes;
Microbiological analysis;
Fermentation control;
Bead-based assays for research purposes;
Particle and cell concentration analysis based on True Volumetric
Absolute Counting;
Particle size and fluorescence distribution analysis.
2.2 Prohibited use
Never use the device with organic solvents as this can destroy the fluidic
system. Only use particles with a diameter < 100 µm. Only use Sysmex
approved fluids for sheath, cleaning and decontamination.
Never for use in diagnostic procedures.
2.3 Reasonably foreseeable misuse
Only use this device as a sensitive measuring device. It should:
NOT be used as personal computer.
NOT be used with chemicals harmful to the device (household sanitisers
etc.).
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 9
2 Safety
2.4 User definition
The device may only be used by personnel properly instructed in its
handling. The manufacturer, authorised distributor or a person authorised by
the operator (and trained by either the manufacturer or an authorised
distributor) must provide instruction in handling the device with the help of
this operating manual.
The device is aimed for a large spectrum of users, from beginners to
experts. In the best case an expert is around – so, if you are a beginner, let
her/him help you.
Access to the device is not permitted to insufficiently qualified personnel.
2.4.1 User qualification groups
Instructed personnel
Instructed personnel have to be trained by qualified personnel or personnel
authorised by Sysmex to operate the device within the scope of the intended
use. They have to be able to assess if the device can be safely used and are
proficient in handling hazardous substances.
Qualified personnel
Qualified personnel have in-depth knowledge of the device and the
applications. They have been trained by personnel authorised by Sysmex to
operate the device, to perform cleaning and certain maintenance operations.
Qualified personnel have a scientific or technical education and several
years of experience in flow cytometry and are proficient in handling
hazardous substances.
Authorised service personnel
Personnel conducting technical service must be authorised by Sysmex.
Please contact the service department of your local Sysmex representative.
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2.4.2 User qualification table
2 Safety
Activity Instructed
personnel
Transport × ×
Installation ×
Operation × × ×
Cleaning × × ×
Decontamination × ×
Maintenance ×¹ ×¹ ×
Technical service ×
Uninstalling × ×
Packing × ×
Disposal × ×
× = activity can be performed by the user
¹ = please refer to the maintenance table for detailed instructions (see
chapter 9 Maintenance on page 44).
Qualified
personnel
Authorised
service
personnel
2.5 Environmental conditions
The device may only be used in laboratories. The specified ambient
temperature and relative humidity conditions must be maintained at all times.
The laboratory environment must be clean. Direct sun light should be
avoided.
CyFlow® Cube 8
Configuration
V1, V2, V4, V5, V6,
V7, V8, V9, V10,
V11
N1 15 °C to 30 °C
Relative humidity: between 20 % and 85 %, non-condensing.
Operating temperature range
15 °C to 30 °C
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 11
2 Safety
2.6 Prohibition and warning signs on the device
The following prohibition and warning signs have been affixed to the device.
Warning sticker Meaning
Laser radiation
Electrical hazards
Beware of electrical currents and electrical shocks
Additional laser labelling
2.7 Personal protective equipment
The following personal protective equipment is recommended to operate the
device.
Gloves must be worn at all times.
Lab coat must be worn at all times.
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2.8 Laser safety
The device is equipped with a class IIIb laser unit. If the housing of the
device is closed, the laser class of the laser unit is classified as a laser
class I (EN 60825-1:2015).
Never open the housing of the device.
Laser light of laser class IIIb can be emitted if the housing of the device is
damaged or the laser protection is removed. Serious injuries of the eyes and
the skin are the results of emission of laser light of laser class IIIb.
2.9 Electrical safety
The main electrical source used must be grounded or earthed due to local
specifications.
2 Safety
Please place the device in such a way that the electrical power cord can
easily be unplugged. Removable power cords should not be replaced by
inadequate wiring.
Beware of possible voltage when changing the fuse (T 4.0 A 250 V).
2.10 Alterations to the device
Unauthorised alterations to the device can result in risks and hazards.
Therefore, unless expressly permitted by the manufacturer, no alterations
may be made to the device.
If the device is altered, appropriate tests and trials must be performed to
ensure the continued safe use of the device.
2.11
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3Overview
5
1
2
3
4
3Overview
Your CyFlow® Cube device is a fully equipped desktop Flow Cytometer
(FCM). Which features a modular optical concept. This allows different
lasers with different wavelength ranges be used as light sources and the
detection of up to 8 optical channels (parameters).
The device allows the optics to be optimised for any application simply by
exchanging optical filters and mirrors. The device runs with an internal PC.
Data acquisition, device control, and data analysis are controlled and
performed by the software.
Fig. 3.1 Front and left side of the device
14 CyFlow
1 On switch
2 Sample port
3 Ports for external devices
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4 Sliding compartment
5 Display
3Overview
1 3
15
14
Fig. 3.2 Ports for external devices
1 USB port
2 HDMI port
3 VGA port
4 Ethernet port
5 USB port
6 Ethernet port
7 Audio port
8 Audio port
2 4 5 6
7
8
1213 91011
9 USB port
10 USB port
11 USB port
12 DVI port
13 USB port
14 Keyboard port
15 Mouse port
1
2
3
Fig. 3.3 Rear side of the device
1 Fuse insert (T 4.0 A 250 V)
2 Power switch
3 Connector socket for the power cord
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 15
3Overview
5
1
2
4
3
Fig. 3.4 Sample port at the right side of the device
1 Start electrode
2 Stop electrode
3 Sample tube
16 CyFlow
4 Sample holder
5 Sample port injection needle
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3.1 Components of the device
The device contains the flow cuvette, the optics, the fluidics, the computer
unit and data acquisition electronics. No work steps inside the device are
necessary.
The main components of the device are:
Control elements
The device is operated via keyboard and mouse. The On switch is located
on the top, when the display of the device is lifted up. The power switch is
located on the rear side of the device.
Computer interface ports
The computer interface ports connect the device with additional optional
electronic devices. They are located on the left side of the device.
3Overview
Sheath fluid bottle and waste bottle
The sheath fluid transports the sample flow during the measurement.
The connecting ports for the tubings of the sheath fluid bottle and waste
bottle are located inside the device. The tubings are preinstalled. The sheath
fluid bottle and waste bottle are located in the sliding compartment at the
rear side of the device.
Sample port with electrodes
The sample port is located on the right side of the device.
The sample port holds the sample tube during the measurement. The start
and stop electrodes of the sample port allow the independent determination
of exact 200 μL volume for each sample. The method of True Volumetric
Absolute Counting (TVAC) supported by CyFlow
the precise measurement of a fixed sample volume.
®
Cube systems is based on
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 17
3Overview
3.2 Type plate
The type plate is attached to the rear side of the device.
The content of the type plate varies according to the system configuration.
The system configuration is represented by an addition to the REF number
(e.g. CY-S-3068R_N1).
The following signs are part of the type plate which has been affixed to the device.
Symbol Meaning
RUO
For Research Use Only
Manufacturer
Certification mark
Reference Number
Serial Number
Manufacturing date
Waste of Electrical and Electronic Equipment
Operating manual
Indicates the temperature limits to which the device can be
safely exposed
No direct sunlight at the installation location
Indicates the range of relative humidity to which the device
can be safely exposed
Caution
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3.3 Scope of delivery
3Overview
1
4 3
Fig. 3.5 Scope of operation
1 CyFlow® Cube device
2 Keyboard and mouse
3 Waste bottle
4 Sheath fluid bottle with inline filter for
sheath fluid
The scope of delivery includes:
1 CyFlow
1 Power cord;
1 Software dongle;
1 Operating manual;
1 Laboratory bottle, 1 litre, labeled with sheath;
1 Laboratory bottle, 1 litre, labeled with waste;
®
Cube device;
2
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3Overview
1 Blue cap with electrodes, cabling and tubing for sheath including inline
filter;
1 Red cap with electrodes, cabling and tubing for waste.
The scope of operation includes:
1 CyFlow
1 Power cord;
1 Software dongle;
1 Operating manual;
1 Keyboard and 1 mouse;
1 Laboratory bottle, 1 liter, labeled with sheath;
1 Laboratory bottle, 1 liter, labeled with waste;
1 Blue cap with electrodes, cabling and tubing for sheath;
®
Cube device;
1 Red cap with electrodes, cabling and tubing for waste.
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3.4 Accessories and spare parts
Additional available application accessories and spare parts:
Product name Content Order No.
Sheath fluid 5000 ml 04-4007_R
Sheath fluid tap 1 piece 04-4006
Cleaning solution 250 ml 04-4009_R
3Overview
Decontamination
250 ml 04-4010_R
solution
Hypochlorite solution 250 ml 04-4012_R
Count Check Beads
50 tests 05-4010
(low, medium, high)
Calibration Beads 1 µm
1 ml 05-4007
Concentrate
Calibration Beads
30 ml 05-4018
3 µm, ready-to-use
DNA Control PI 25 ml 05-7303
DNA Control UV 25 ml 05-7302
Sheath fluid bottle 1l 1000 ml empty bottle 04-200-1041
Waste bottle 1l 1000 ml empty bottle 04-200-1042
Cap for 1 l glass
1 piece 04-200-1056
sheath fluid bottle
Cap for 1 l glass
1 piece 04-200-1057
waste bottle
Sample tube 3.5 ml 500 pieces 04-2000
Keyboard 1 piece 06-500-1040
Mouse 1 piece 06-500-1041
Spare fuses T 4,0 A 5 pieces 06-7-8006
Additional available upgrades:
CyFlow
CyFlow® Sorter for CyFlow
®
Robby 8 Autoloading Station
®
Cube
Visit our website http://www.sysmex-partec.com or contact your local
Sysmex representative for further information about our products.
3.5
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4 Transport and storage
4 Transport and storage
Warning! The weight of the device is about 40 kg.
Always have at least two people carry or lift the device.
In order to transport the device to a different location all external data and
supply connections have to be disconnected.
The device should be carried in an upright position.
The device is supplied in packaging that protects it against damage and
contamination. Please retain the packaging. Place the device in the
packaging for transport and storage.
The storage environment must be dry, clean and dust-free:
Storage temperature: between 5 °C and 50 °C;
Relative humidity: between 20 % and 85 %, non-condensing.
4.1
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5 Installation
5.1 Positioning the device
Heavy weight
When moving the device (packed or unpacked) or removing it from its
packaging, a person is exposed to a high risk of damaging his or her body
due to the heavy weight of the device.
At least two persons are required for transport and installation.
Carefully select the place where to position and operate the device. Comply
with the following criteria when positioning the device:
5 Installation
Place the device on a solid, dry and clean horizontal surface.
Avoid smoke, dust, vibrations, direct sunlight and any source of direct
heat.
Leave a minimum distance of 15 cm between the back of the device and
a wall to keep the ventilation effective.
Do not block the ventilation grid at the back of the device.
Do not place any objects on the device.
5.2 Installation and uninstallation
Installation, connection, disconnection and uninstallation may only be
performed by authorised service personnel and in conformity with the
applicable national rules and regulations.
5.3
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6 Operation
6Operation
Due to heavy use of software-related content in the following chapter please
also refer to chapter 12 Software description on page 50.
6.1 Inserting the Software dongle
Procedure
1. Insert the included Software dongle into an open USB port of the device.
Result
The software will check automatically in regular intervals if the dongle is
plugged in. If the dongle is not plugged in, the software will not be
functional.
6.2 Start-up procedure
Old sheath fluid
Old sheath fluid affects the sample flow through the flow cuvette.
Replace the sheath fluid at least once a week or before any daily use.
6.2.1 Switching on the device
Before switching on the device make sure the sheath fluid bottle is filled with
800 ml of clean, filtered and degassed sheath fluid and is closed with the
blue screw cap. It is recommended to replace the sheath fluid at least once a
week or before any daily use.
Requirements
The sheath fluid bottle is filled and connected to the device.
The waste bottle is empty and connected to the device.
Procedure
1. Switch on the device using the power switch on the rear side of the device.
2. Lift up the display of the device.
3. Push the On switch on the top of the device.
– The device is switched on.
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4. Start the software by double click the CyFlow® Software icon.
– The log-in dialogue is displayed.
– The device is, by default, set in a standard mode.
6.2.2 Logging in and logging out
Each user is assigned to a user role. Each user role has specific privileges.
Some functions of the software are not available for each user role and
availability depends on the assigned privileges.
User management
Role Privileges
Service operator User administration
6Operation
Worklist creation
Logging in
Worklist management
Sample analysis
Manage Workspace and settings
Create comp setup
User administrator User administration
Parameter properties
Assay developer Manage Workspace and settings
Worklist creation
Worklist management
Sample analysis
Create comp setup
Requirements
The log-in dialogue is displayed.
User access must be set up and the user name and password must be
known to log into the software.
Procedure
1. Enter the user name.
2. Enter the password.
3. Click the Log-in button.
– The log-in dialogue closes.
– The user role dialogue will be displayed.
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6 Operation
Logging out
4. Select the respective user role.
5. Click the OK button.
– If the log-in was successful the start screen is displayed.
After a specified number of unsuccessful login attempts (default 3), the
account will be locked. If the account is locked, please contact a user with
the "user administration" privilege in its user role, e. g. a user administrator.
The default settings for logins can be changed in the user management.
Procedure
1. On the File tab of the Ribbon (see chapter 12.4.1 File tab on page 58)
click the Log-out button.
– The current user is logged out.
– The log-in dialogue is displayed.
6.2.3 Performing a Prime
A Prime mode is available as a start-up process for the device. This process
includes cleaning, filling the tubes with sheath fluid and controlling the device
set-up.
The Prime should be performed in the following cases:
Starting the device for the first time of the day,
After the sheath fluid bottle has been filled,
As a trouble-shooting procedure (no/bad signals, blocking, etc.).
Requirements
Sample tube with 1.6 ml Decontamination Solution (Order No.
04-4010_R) connected.
Procedure
1. On the Cytometer tab of the Ribbon (see chapter 12.4.10 Cytometer tab
on page 105) or on the Acquire pane (see chapter 12.5.4 Acquire pane
on page 140) click the Prime button.
– The instruction dialogue for the Prime procedure is displayed.
2. Follow the instructions until the finish message is displayed.
– The device is ready for the sample measurements.
3. Remove emptied sample tube.
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6.3 Performing a measurement
Requirements
The device is primed.
The respective Worklist is loaded.
A Worklist item is active with a defined Workspace.
The Worklist contains at least one defined item that does not contain a
listmode file.
The sample is prepared for the measurement.
The sheath fluid is not low.
The waste level is not high.
Procedure
6Operation
1. Fill the sample tube with the sample fluid.
2. Insert the sample tube into the sample port of the device until the sample tube audibly clicks.
3. On the Acquire pane (see chapter 12.5.4 Acquire pane on page 140)
click the Start button.
– Data acquisition starts. Progress is displayed in the status bar.
4. If a stop condition is set, wait until acquisition stops.
If no stop condition is set, click the Stop button on the Acquire pane to
stop acquisition.
– A measurement is performed.
5. Remove emptied sample tube.
CyFlow® Cube 8 | Operating Manual | May 2018 | Revision 032 27
6 Operation
6.4 Performing an intermediate cleaning
A cleaning mode is available to clean the device when a different sample
type is to be measured.
Requirements
Log-in as a user with the respective role.
The respective Worklist is loaded.
Sample tube with 1.6 ml Cleaning Solution (Order No. 04-4009_R)
connected.
Procedure
1. On the Cytometer tab of the Ribbon (see chapter 12.4.10 Cytometer tab
on page 105) click the Clean button.
– An instruction dialogue is displayed.
2. Follow the instructions until the finish message is displayed.
– An intermediate cleaning was performed.
– The device is ready for the next sample measurement.
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6.5 Shutdown procedure
6.5.1 Cleaning for shutdown
Perform the shutdown cleaning procedure to close the software.
Requirements
No acquisition is in progress.
Sample tube with 1.6 ml Decontamination Solution (Order No.
04-4010_R) connected.
Procedure
1. On the File tab of the Ribbon (see chapter 12.4.1 File tab on page 58)
click the Exit CyFlow Software button.
– If the Worklist or Playlist is currently open and has been modified, a
save changes dialogue appears.
6Operation
– A dialogue asking for a shutdown cleaning procedure appears.
2. Select Yes in the dialogue.
– The dialogue closes and initiates the shutdown cleaning procedure.
– After the cleaning procedure is complete the software closes.
3. Remove emptied sample tube.
6.5.2 Switching off the device
Requirements
The shutdown cleaning procedure was successful and the software is
closed.
Procedure
1. In the Windows menu, click the Shut down button.
– The device shuts down.
2. Switch off the device using the power switch on the rear side of the device.
– The device is switched off.
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6 Operation
6.6 Software operation
The software can be run in two different modes:
In Worklist mode, data can be acquired. The hardware and software
settings for each measurement are predefined and organised in a list
called Worklist.
In Playlist mode, acquired data can be analysed and settings for future
measurements can be prepared. Files are organised and edited in a
Playlist.
This chapter describes how to use a Worklist for preparing sample
measurements and how to use a Playlist to analyse data. For a detailed
description of software functions, see chapter 12 Software description on
page 50.
6.6.1 Preparing measurements using the Worklist
This chapter describes the typical sequence of steps when preparing a
Worklist for future measurements.
A Worklist contains the predefinitions of hardware and software settings for
data acquisition (see chapter 12.6 Worklist pane on page 146). These
predefinitions are set prior to measurements when preparing the Worklist.
Preparing the Worklist includes:
Defining the Workspace that contains the plots, regions and gates to
represent the cell populations of interest;
Defining and adapting the instrument settings to reduce background and
to position the populations of interest properly into the plots;
Performing a manual compensation to compensate for fluorescence
spillover as appropriate.
These settings can be preset prior to acquiring data and adapted while
measuring samples.
30 CyFlow
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Cube 8 | Operating Manual | May 2018 | Revision 032
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