Roth HP22.1 User guide

1

HP22.1 Roth Random Primer Kit A für die RAPD-PCR

20 x 10mer

Kit besteht aus 20 Einzelprimern, jeweils aufgeteilt auf 2 Reaktionsgefäße zu je 1,0 OD

Achtung: Angaben beziehen sich jeweils auf ein Reaktionsgefäß!

Primer

Sequenz 5'  3'

MW (g/mol)

Liefermenge (1,0 OD)

Molarer

Extinkt.koeffiz.

(l/mol∙cm)

Konz. bei

Lösg. in 1 ml

(pmol/µl)

Lösungsmittelvolumen

für Konzentration

100 pmol/µl

Schmelzpunkt

( 2° C + 4° C-Regel)

Schmelzpunkt

(G/C-Regel)

01

CAG GCC CTT C

2955

31,4 µg / 10,6 nmol

91.971

10,6

106,2 µl

34,0 °C

21,1 °C

02

TGC CGA GCT G

3035

29,1 µg / 9,6 nmol

100.683

9,6

96,0 µl

34,0 °C

21,1 °C

03

AGT CAG CCA C

2988

27,3 µg / 9,1 nmol

106.524

9,1

91,2 µl

32,0 °C

17,0 °C

04

AAT CGG GCT G

3059

27,0 µg / 8,8 nmol

108.702

8,8

88,3 µl

32,0 °C

17,0 °C

05

AGG GGT CTT G

3090

27,6 µg / 8,9 nmol

106.524

8,9

89,4 µl

32,0 °C

17,0 °C

06

GGT CCC TGA C

2995

30,2 µg / 10,1 nmol

96.327

10,1

100,8 µl

34,0 °C

21,1 °C

07

GAA ACG GGT G

3108

24,9 µg / 8,0 nmol

119.592

8,0

80,3 µl

32,0 °C

17,0 °C

08

GTG ACG TAG G

3099

26,2 µg / 8,5 nmol

113.058

8,5

84,6 µl

32,0 °C

17,0 °C

09

GGG TAA CGC C

3044

27,5 µg / 9,0 nmol

107.217

9,0

90,4 µl

34,0 °C

21,1 °C

10

GTG ATC GCA G

3059

27,0 µg / 8,8 nmol

108.702

8,8

88,3 µl

32,0 °C

17,0 °C

11

CAA TCG CCG T

2979

28,9 µg / 9,7 nmol

99.990

9,7

96,9 µl

32,0 °C

17,0 °C

12

TCG GCG ATA G

3059

27,0 µg / 8,8 nmol

108.702

8,8

88,3 µl

32,0 °C

17,0 °C

13

CAG CAC CCA C

2933

28,8 µg / 9,8 nmol

100.683

9,8

98,0 µl

34,0 °C

21,1 °C

14

TCT GTG CTG G

3041

30,3 µg / 10,0 nmol

95.634

10,0

99,6 µl

32,0 °C

17,0 °C

15

TTC CGA ACC C

2939

29,9 µg / 10,2 nmol

95.634

10,2

101,8 µl

32,0 °C

17,0 °C

16

AGC CAG CGA A

3037

25,1 µg / 8,3 nmol

117.414

8,3

82,6 µl

32,0 °C

17,0 °C

17

GAC CGC TTG T

3010

29,6 µg / 9,8 nmol

97.812

9,8

98,2 µl

32,0 °C

17,0 °C

18

AGG TGA CCG T

3059

27,0 µg / 8,8 nmol

108.702

8,8

88,3 µl

32,0 °C

17,0 °C

19

CAA ACG TCG G

3028

26,4 µg / 8,7 nmol

110.880

8,7

87,3 µl

32,0 °C

17,0 °C

20

GTT GCG ATC C

3010

29,6 µg / 9,8 nmol

97.812

9,8

98,2 µl

32,0 °C

17,0 °C

Gebrauchsanweisung

2

HP23.1 Roth Random Primer Kit B für die RAPD-PCR

20 x 10mer

Kit besteht aus 20 Einzelprimern, jeweils aufgeteilt auf 2 Reaktionsgefäße zu je 1,0 OD

Achtung: Angaben beziehen sich jeweils auf ein Reaktionsgefäß!

Primer

Sequenz 5'  3'

MW (g/mol)

Liefermenge (1,0 OD)

Molarer

Extinkt.koeffiz.

(l/mol∙cm)

Konz. bei

Lösg. in 1 ml

(pmol/µl)

Lösungsmittelvol. für

Konzentration

100 pmol/µl

Schmelzpunkt

( 2° C + 4° C-Regel)

Schmelzpunkt

(G/C-Regel)

01

GTT TCG CTC C

2961

32,8 µg / 11,1 nmol

86.922

11,1

110,6 µl

32,0 °C

17,0 °C

02

TGA TCC CTG G

3010

29,6 µg / 9,8 nmol

97.812

9,8

98,2 µl

32,0 °C

17,0 °C

03

CAT CCC CCT G

2915

32,7 µg / 11,2 nmol

87.615

11,2

112,1 µl

34,0 °C

21,1 °C

04

GGA CTG GAG T

3099

26,2 µg / 8,5 nmol

113.058

8,5

84,6 µl

32,0 °C

17,0 °C

05

TGC GCC CTT C

2946

33,6 µg / 11,4 nmol

85.437

11,4

113,9 µl

34,0 °C

21,1 °C

06

TGC TCT GCC C

2946

33,6 µg / 11,4 nmol

85.437

11,4

113,9 µl

34,0 °C

21,1 °C

07

GGT GAC GCA G

3084

26,7 µg / 8,7 nmol

111.573

8,7

86,5 µl

34,0 °C

21,1 °C

08

GTC CAC ACG G

3004

28,4 µg / 9,5 nmol

102.861

9,5

94,7 µl

34,0 °C

21,1 °C

09

TGG GGG ACT C

3075

28,2 µg / 9,2 nmol

105.039

9,2

91,6 µl

34,0 °C

21,1 °C

10

CTG CTG GGA C

3035

29,1 µg / 9,6 nmol

100.683

9,6

96,0 µl

34,0 °C

21,1 °C

11

GTA GAC CCG T

3019

27,9 µg / 9,2 nmol

104.346

9,2

92,4 µl

32,0 °C

17,0 °C

12

CCT TGA CGC A

2979

28,9 µg / 9,7 nmol

99.990

9,7

96,9 µl

32,0 °C

17,0 °C

13

TTC CCC CGC T

2906

35,1 µg / 12,1 nmol

81.081

12,1

120,8 µl

34,0 °C

21,1 °C

14

TCC GCT CTG G

2986

32,2 µg / 10,8 nmol

89.793

10,8

107,8 µl

34,0 °C

21,1 °C

15

GGA GGG TGT T

3130

26,8 µg / 8,6 nmol

110.880

8,6

85,6 µl

32,0 °C

17,0 °C

16

TTT GCC CGG A

3010

29,6 µg / 9,8 nmol

97.812

9,8

98,2 µl

32,0 °C

17,0 °C

17

AGG GAA CGA G

3117

23,8 µg / 7,6 nmol

126.126

7,6

76,3 µl

32,0 °C

17,0 °C

18

CCA CAG CAG T

2988

27,3 µg / 9,1 nmol

106.524

9,1

91,2 µl

32,0 °C

17,0 °C

19

ACC CCC GAA G

2973

27,8 µg / 9,3 nmol

105.039

9,3

93,5 µl

34,0 °C

21,1 °C

20

GGA CCC TTA C

2979

28,9 µg / 9,7 nmol

99.990

9,7

96,9 µl

32,0 °C

17,0 °C

3

HP24.1 Roth Random Primer Kit C für die RAPD-PCR 20 x 10mer

Kit besteht aus 20 Einzelprimern, jeweils aufgeteilt auf 2 Reaktionsgefäße zu je 1,0 OD

Achtung: Angaben beziehen sich jeweils auf ein Reaktionsgefäß!

Primer

Sequenz 5'  3'

MW (g/mol)

Liefermenge (1,0 OD)

Molarer

Extinkt.koeffiz.

(l/mol∙cm)

Konz. bei

Lösg. in 1 ml

(pmol/µl)

Lösungsmittelvol. für

Konzentration

100 pmol/µl

Schmelzpunkt

( 2° C + 4° C-Regel)

Schmelzpunkt

(G/C-Regel)

01

TTC GAG CCA G

3019

27,9 µg / 9,2 nmol

104.346

9,2

92,4 µl

32,0 °C

17,0 °C

02

GTG AGG CGT C

3075

28,2 µg / 9,2 nmol

105.039

9,2

91,6 µl

34,0 °C

21,1 °C

03

GGG GGT CTT T

3081

29,2 µg / 9,5 nmol

99.990

9,5

94,9 µl

32,0 °C

17,0 °C

04

CCG CAT CTA C

2939

29,9 µg / 10,2 nmol

95.634

10,2

101,8 µl

32,0 °C

17,0 °C

05

GAT GAC CGC C

3004

28,4 µg / 9,5 nmol

102.861

9,5

94,7 µl

34,0 °C

21,1 °C

06

GAA CGG ACT C

3028

26,4 µg / 8,7 nmol

110.880

8,7

87,3 µl

32,0 °C

17,0 °C

07

GTC CCG ACG A

3004

28,4 µg / 9,5 nmol

102.861

9,5

94,7 µl

34,0 °C

21,1 °C

08

TGG ACC GGT G

3075

28,2 µg / 9,2 nmol

105.039

9,2

91,6 µl

34,0 °C

21,1 °C

09

CTC ACC GTC C

2915

32,7 µg / 11,2 nmol

87.615

11,2

112,1 µl

34,0 °C

21,1 °C

10

TGT CTG GGT G

3081

29,2 µg / 9,5 nmol

99.990

9,5

94,9 µl

32,0 °C

17,0 °C

11

AAA GCT GCG G

3068

25,7 µg / 8,4 nmol

115.236

8,4

83,6 µl

32,0 °C

17,0 °C

12

TGT CAT CCC C

2930

31,9 µg / 10,9 nmol

89.100

10,9

108,9 µl

32,0 °C

17,0 °C

13

AAG CCT CGT C

2979

28,9 µg / 9,7 nmol

99.990

9,7

96,9 µl

32,0 °C

17,0 °C

14

TGC GTG CTT G

3041

30,3 µg / 10,0 nmol

95.634

10,0

99,6 µl

32,0 °C

17,0 °C

15

GAC GGA TCA G

3068

25,7 µg / 8,4 nmol

115.236

8,4

83,6 µl

32,0 °C

17,0 °C

16

CAC ACT CCA G

2948

28,2 µg / 9,6 nmol

102.168

9,6

95,6 µl

32,0 °C

17,0 °C

17

TTC CCC CCA G

2915

32,7 µg / 11,2 nmol

87.615

11,2

112,1 µl

34,0 °C

21,1 °C

18

TGA GTG GGT G

3130

26,8 µg / 8,6 nmol

110.880

8,6

85,6 µl

32,0 °C

17,0 °C

19

GTT GCC AGC C

2995

30,2 µg / 10,1 nmol

96.327

10,1

100,8 µl

34,0 °C

21,1 °C

20

ACT TCG CCA C

2939

29,9 µg / 10,2 nmol

95.634

10,2

101,8 µl

32,0 °C

17,0 °C

4

Roth Random Primer Kit A für die RAPD-PCR 20x 10 mer HP22.1 Roth Random Primer Kit B für die RAPD-PCR 20x 10 mer HP23.1 Roth Random Primer Kit C für die RAPD-PCR 20x 10 mer HP24.1 Roth Random Primer Kit D für die RAPD-PCR 20x 10 mer HP25.1

HP25.1 Roth Random Primer Kit D für die RAPD-PCR

20 x 10mer

Kit besteht aus 20 Einzelprimern, jeweils aufgeteilt auf 2 Reaktionsgefäße zu je 1,0 OD

Achtung: Angaben beziehen sich jeweils auf ein Reaktionsgefäß!

Primer

Sequenz 5'  3'

MW (g/mol)

Liefermenge (1,0 OD)

Molarer

Extinkt.koeffiz.

(l/mol∙cm)

Konz. bei

Lösg. in 1 ml

(pmol/µl)

Lösungsmittelvol. für

Konzentration

100 pmol/µl

Schmelzpunkt

( 2° C + 4° C-Regel)

Schmelzpunkt

(G/C-Regel)

01

ACC GCG AAG G

3053

26,1 µg / 8,5 nmol

113.751

8,5

85,5 µl

34,0 °C

21,1 °C

02

GGA CCC AAC C

2973

27,8 µg / 9,3 nmol

105.039

9,3

93,5 µl

34,0 °C

21,1 °C

03

GTC GCC GTC A

2995

30,2 µg / 10,1 nmol

96.327

10,1

100,8 µl

34,0 °C

21,1 °C

04

TCT GGT GAG G

3090

27,6 µg / 8,9 nmol

106.524

8,9

89,4 µl

32,0 °C

17,0 °C

05

TGA GCG GAC A

3068

25,7 µg / 8,4 nmol

115.236

8,4

83,6 µl

32,0 °C

17,0 °C

06

ACC TGA ACG G

3028

26,4 µg / 8,7 nmol

110.880

8,7

87,3 µl

32,0 °C

17,0 °C

07

TTG GCA CGG G

3075

28,2 µg / 9,2 nmol

105.039

9,2

91,6 µl

34,0 °C

21,1 °C

08

GTG TGC CCC A

2995

30,2 µg / 10,1 nmol

96.327

10,1

100,8 µl

34,0 °C

21,1 °C

09

CTC TGG AGA C

3019

27,9 µg / 9,2 nmol

104.346

9,2

92,4 µl

32,0 °C

17,0 °C

10

GGT CTA CAC C

2979

28,9 µg / 9,7 nmol

99.990

9,7

96,9 µl

32,0 °C

17,0 °C

11

AGC GCC ATT G

3019

27,9 µg / 9,2 nmol

104.346

9,2

92,4 µl

32,0 °C

17,0 °C

12

CAC CGT ATC C

2939

29,9 µg / 10,2 nmol

95.634

10,2

101,8 µl

32,0 °C

17,0 °C

13

GGG GTG ACG A

3124

25,9 µg / 8,3 nmol

115.929

8,3

82,9 µl

34,0 °C

21,1 °C

14

CTT CCC CAA G

2939

29,9 µg / 10,2 nmol

95.634

10,2

101,8 µl

32,0 °C

17,0 °C

15

CAT CCG TGC T

2970

30,7 µg / 10,3 nmol

93.456

10,3

103,3 µl

32,0 °C

17,0 °C

16

AGG GCG TAA G

3108

24,9 µg / 8,0 nmol

119.592

8,0

80,3 µl

32,0 °C

17,0 °C

17

TTT CCC ACG G

2970

30,7 µg / 10,3 nmol

93.456

10,3

103,3 µl

32,0 °C

17,0 °C

18

GAG AGC CAA C

3037

25,1 µg / 8,3 nmol

117.414

8,3

82,6 µl

32,0 °C

17,0 °C

19

CTG GGG ACT T

3050

28,6 µg / 9,4 nmol

102.168

9,4

93,6 µl

32,0 °C

17,0 °C

20

ACC CGG TCA C

2964

29,5 µg / 9,9 nmol

98.505

9,9

99,4 µl

34,0 °C

21,1 °C

5

HP22.1 Roth Random Primer Kit A for RAPD-PCR

20 x 10mer

Kit contains 20 primers, aliquoted in 2 vials, each with 1.0 OD

Please note: Information below refers to one vial each!

Primer

Sequence 5'  3'

MW (g/mol)

Amount (1.0 OD)

Molar

extinct. coeffic.

(l/mol∙cm)

Conc. when

solubilized in

1 ml (pmol/µl)

Required solvent

volume for conc.

100 pmol/µl

Melting point

( 2° C + 4° C rule)

Melting point

(G/C rule)

01

CAG GCC CTT C

2955

31.4 µg / 10.6 nmol

91971

10.6

106.2 µl

34.0 °C

21.1 °C

02

TGC CGA GCT G

3035

29.1 µg / 9.6 nmol

100683

9.6

96.0 µl

34.0 °C

21.1 °C

03

AGT CAG CCA C

2988

27.3 µg / 9.1 nmol

106524

9.1

91.2 µl

32.0 °C

17.0 °C

04

AAT CGG GCT G

3059

27.0 µg / 8.8 nmol

108702

8.8

88.3 µl

32.0 °C

17.0 °C

05

AGG GGT CTT G

3090

27.6 µg / 8.9 nmol

106524

8.9

89.4 µl

32.0 °C

17.0 °C

06

GGT CCC TGA C

2995

30.2 µg / 10.1 nmol

96327

10.1

100.8 µl

34.0 °C

21.1 °C

07

GAA ACG GGT G

3108

24.9 µg / 8.0 nmol

119592

8.0

80.3 µl

32.0 °C

17.0 °C

08

GTG ACG TAG G

3099

26.2 µg / 8.5 nmol

113058

8.5

84.6 µl

32.0 °C

17.0 °C

09

GGG TAA CGC C

3044

27.5 µg / 9.0 nmol

107217

9.0

90.4 µl

34.0 °C

21.1 °C

10

GTG ATC GCA G

3059

27.0 µg / 8.8 nmol

108702

8.8

88.3 µl

32.0 °C

17.0 °C

11

CAA TCG CCG T

2979

28.9 µg / 9.7 nmol

99990

9.7

96.9 µl

32.0 °C

17.0 °C

12

TCG GCG ATA G

3059

27.0 µg / 8.8 nmol

108702

8.8

88.3 µl

32.0 °C

17.0 °C

13

CAG CAC CCA C

2933

28.8 µg / 9.8 nmol

100683

9.8

98.0 µl

34.0 °C

21.1 °C

14

TCT GTG CTG G

3041

30.3 µg / 10.0 nmol

95634

10.0

99.6 µl

32.0 °C

17.0 °C

15

TTC CGA ACC C

2939

29.9 µg / 10.2 nmol

95634

10.2

101.8 µl

32.0 °C

17.0 °C

16

AGC CAG CGA A

3037

25.1 µg / 8.3 nmol

117414

8.3

82.6 µl

32.0 °C

17.0 °C

17

GAC CGC TTG T

3010

29.6 µg / 9.8 nmol

97812

9.8

98.2 µl

32.0 °C

17.0 °C

18

AGG TGA CCG T

3059

27.0 µg / 8.8 nmol

108702

8.8

88.3 µl

32.0 °C

17.0 °C

19

CAA ACG TCG G

3028

26.4 µg / 8.7 nmol

110880

8.7

87.3 µl

32.0 °C

17.0 °C

20

GTT GCG ATC C

3010

29.6 µg / 9.8 nmol

97812

9.8

98.2 µl

32.0 °C

17.0 °C

Instructions for use

6

HP23.1 Roth Random Primer Kit B for RAPD-PCR 20 x 10mer

Kit contains 20 primers, aliquoted in 2 vials, each with 1.0 OD

Please note: Information below refers to one vial each!

Primer

Sequence 5'  3'

MW (g/mol)

Amount (1.0 OD)

Molar

extinct. coeffic.

(l/mol∙cm)

Conc., when

solubilized in

1 ml (pmol/µl)

Required solvent

volume for conc.

100 pmol/µl

Melting point

( 2° C + 4° C rule)

Melting point

(G/C rule)

01

GTT TCG CTC C

2961

32.8 µg / 11.1 nmol

86922

11.1

110.6 µl

32.0 °C

17.0 °C

02

TGA TCC CTG G

3010

29.6 µg / 9.8 nmol

97812

9.8

98.2 µl

32.0 °C

17.0 °C

03

CAT CCC CCT G

2915

32.7 µg / 11.2 nmol

87615

11.2

112.1 µl

34.0 °C

21.1 °C

04

GGA CTG GAG T

3099

26.2 µg / 8.5 nmol

113058

8.5

84.6 µl

32.0 °C

17.0 °C

05

TGC GCC CTT C

2946

33.6 µg / 11.4 nmol

85437

11.4

113.9 µl

34.0 °C

21.1 °C

06

TGC TCT GCC C

2946

33.6 µg / 11.4 nmol

85437

11.4

113.9 µl

34.0 °C

21.1 °C

07

GGT GAC GCA G

3084

26.7 µg / 8.7 nmol

111573

8.7

86.5 µl

34.0 °C

21.1 °C

08

GTC CAC ACG G

3004

28.4 µg / 9.5 nmol

102861

9.5

94.7 µl

34.0 °C

21.1 °C

09

TGG GGG ACT C

3075

28.2 µg / 9.2 nmol

105039

9.2

91.6 µl

34.0 °C

21.1 °C

10

CTG CTG GGA C

3035

29.1 µg / 9.6 nmol

100683

9.6

96.0 µl

34.0 °C

21.1 °C

11

GTA GAC CCG T

3019

27.9 µg / 9.2 nmol

104346

9.2

92.4 µl

32.0 °C

17.0 °C

12

CCT TGA CGC A

2979

28.9 µg / 9.7 nmol

99990

9.7

96.9 µl

32.0 °C

17.0 °C

13

TTC CCC CGC T

2906

35.1 µg / 12.1 nmol

81081

12.1

120.8 µl

34.0 °C

21.1 °C

14

TCC GCT CTG G

2986

32.2 µg / 10.8 nmol

89793

10.8

107.8 µl

34.0 °C

21.1 °C

15

GGA GGG TGT T

3130

26.8 µg / 8.6 nmol

110880

8.6

85.6 µl

32.0 °C

17.0 °C

16

TTT GCC CGG A

3010

29.6 µg / 9.8 nmol

97812

9.8

98.2 µl

32.0 °C

17.0 °C

17

AGG GAA CGA G

3117

23.8 µg / 7.6 nmol

126126

7.6

76.3 µl

32.0 °C

17.0 °C

18

CCA CAG CAG T

2988

27.3 µg / 9.1 nmol

106524

9.1

91.2 µl

32.0 °C

17.0 °C

19

ACC CCC GAA G

2973

27.8 µg / 9.3 nmol

105039

9.3

93.5 µl

34.0 °C

21.1 °C

20

GGA CCC TTA C

2979

28.9 µg / 9.7 nmol

99990

9.7

96.9 µl

32.0 °C

17.0 °C

7

HP24.1 Roth Random Primer Kit C for RAPD-PCR 20 x 10mer

Kit contains 20 primers, aliquoted in 2 vials, each with 1.0 OD

Please note: Information below refers to one vial each!

Primer

Sequence 5'  3'

MW (g/mol)

Amount (1.0 OD)

Molar

extinct. coeffic.

(l/mol∙cm)

Conc., when

solubilized in

1 ml (pmol/µl)

Required solvent

volume for conc.

100 pmol/µl

Melting point

( 2° C + 4° C rule)

Melting point

(G/C rule)

01

TTC GAG CCA G

3019

27.9 µg / 9.2 nmol

104346

9.2

92.4 µl

32.0 °C

17.0 °C

02

GTG AGG CGT C

3075

28.2 µg / 9.2 nmol

105039

9.2

91.6 µl

34.0 °C

21.1 °C

03

GGG GGT CTT T

3081

29.2 µg / 9.5 nmol

99990

9.5

94.9 µl

32.0 °C

17.0 °C

04

CCG CAT CTA C

2939

29.9 µg / 10.2 nmol

95634

10.2

101.8 µl

32.0 °C

17.0 °C

05

GAT GAC CGC C

3004

28.4 µg / 9.5 nmol

102861

9.5

94.7 µl

34.0 °C

21.1 °C

06

GAA CGG ACT C

3028

26.4 µg / 8.7 nmol

110880

8.7

87.3 µl

32.0 °C

17.0 °C

07

GTC CCG ACG A

3004

28.4 µg / 9.5 nmol

102861

9.5

94.7 µl

34.0 °C

21.1 °C

08

TGG ACC GGT G

3075

28.2 µg / 9.2 nmol

105039

9.2

91.6 µl

34.0 °C

21.1 °C

09

CTC ACC GTC C

2915

32.7 µg / 11.2 nmol

87615

11.2

112.1 µl

34.0 °C

21.1 °C

10

TGT CTG GGT G

3081

29.2 µg / 9.5 nmol

99990

9.5

94.9 µl

32.0 °C

17.0 °C

11

AAA GCT GCG G

3068

25.7 µg / 8.4 nmol

115236

8.4

83.6 µl

32.0 °C

17.0 °C

12

TGT CAT CCC C

2930

31.9 µg / 10.9 nmol

89100

10.9

108.9 µl

32.0 °C

17.0 °C

13

AAG CCT CGT C

2979

28.9 µg / 9.7 nmol

99990

9.7

96.9 µl

32.0 °C

17.0 °C

14

TGC GTG CTT G

3041

30.3 µg / 10.0 nmol

95634

10.0

99.6 µl

32.0 °C

17.0 °C

15

GAC GGA TCA G

3068

25.7 µg / 8.4 nmol

115236

8.4

83.6 µl

32.0 °C

17.0 °C

16

CAC ACT CCA G

2948

28.2 µg / 9.6 nmol

102168

9.6

95.6 µl

32.0 °C

17.0 °C

17

TTC CCC CCA G

2915

32.7 µg / 11.2 nmol

87615

11.2

112.1 µl

34.0 °C

21.1 °C

18

TGA GTG GGT G

3130

26.8 µg / 8.6 nmol

110880

8.6

85.6 µl

32.0 °C

17.0 °C

19

GTT GCC AGC C

2995

30.2 µg / 10.1 nmol

96327

10.1

100.8 µl

34.0 °C

21.1 °C

20

ACT TCG CCA C

2939

29.9 µg / 10.2 nmol

95634

10.2

101.8 µl

32.0 °C

17.0 °C

8

HP25.1 Roth Random Primer Kit D for RAPD-PCR 20 x 10mer

Kit contains 20 primers, aliquoted in 2 vials, each with 1.0 OD

Please note: Information below refers to one vial each!

Primer

Sequence 5'  3'

MW (g/mol)

Amount (1.0 OD)

Molar

extinct. coeffic.

(l/mol∙cm)

Conc., when

solubilized in

1 ml (pmol/µl)

Required solvent

volume for conc.

100 pmol/µl

Melting point

( 2° C + 4° C rule)

Melting point

(G/C rule)

01

ACC GCG AAG G

3053

26.1 µg / 8.5 nmol

113751

8.5

85.5 µl

34.0 °C

21.1 °C

02

GGA CCC AAC C

2973

27.8 µg / 9.3 nmol

105039

9.3

93.5 µl

34.0 °C

21.1 °C

03

GTC GCC GTC A

2995

30.2 µg / 10.1 nmol

96327

10.1

100.8 µl

34.0 °C

21.1 °C

04

TCT GGT GAG G

3090

27.6 µg / 8.9 nmol

106524

8.9

89.4 µl

32.0 °C

17.0 °C

05

TGA GCG GAC A

3068

25.7 µg / 8.4 nmol

115236

8.4

83.6 µl

32.0 °C

17.0 °C

06

ACC TGA ACG G

3028

26.4 µg / 8.7 nmol

110880

8.7

87.3 µl

32.0 °C

17.0 °C

07

TTG GCA CGG G

3075

28.2 µg / 9.2 nmol

105039

9.2

91.6 µl

34.0 °C

21.1 °C

08

GTG TGC CCC A

2995

30.2 µg / 10.1 nmol

96327

10.1

100.8 µl

34.0 °C

21.1 °C

09

CTC TGG AGA C

3019

27.9 µg / 9.2 nmol

104346

9.2

92.4 µl

32.0 °C

17.0 °C

10

GGT CTA CAC C

2979

28.9 µg / 9.7 nmol

99990

9.7

96.9 µl

32.0 °C

17.0 °C

11

AGC GCC ATT G

3019

27.9 µg / 9.2 nmol

104346

9.2

92.4 µl

32.0 °C

17.0 °C

12

CAC CGT ATC C

2939

29.9 µg / 10.2 nmol

95634

10.2

101.8 µl

32.0 °C

17.0 °C

13

GGG GTG ACG A

3124

25.9 µg / 8.3 nmol

115929

8.3

82.9 µl

34.0 °C

21.1 °C

14

CTT CCC CAA G

2939

29.9 µg / 10.2 nmol

95634

10.2

101.8 µl

32.0 °C

17.0 °C

15

CAT CCG TGC T

2970

30.7 µg / 10.3 nmol

93456

10.3

103.3 µl

32.0 °C

17.0 °C

16

AGG GCG TAA G

3108

24.9 µg / 8.0 nmol

119592

8.0

80.3 µl

32.0 °C

17.0 °C

17

TTT CCC ACG G

2970

30.7 µg / 10.3 nmol

93456

10.3

103.3 µl

32.0 °C

17.0 °C

18

GAG AGC CAA C

3037

25.1 µg / 8.3 nmol

117414

8.3

82.6 µl

32.0 °C

17.0 °C

19

CTG GGG ACT T

3050

28.6 µg / 9.4 nmol

102168

9.4

93.6 µl

32.0 °C

17.0 °C

20

ACC CGG TCA C

2964

29.5 µg / 9.9 nmol

98505

9.9

99.4 µl

34.0 °C

21.1 °C

Roth Random Primer Kit A for RAPD-PCR 20x 10 mer HP22.1 Roth Random Primer Kit B for RAPD-PCR 20x 10 mer HP23.1 Roth Random Primer Kit C for RAPD-PCR 20x 10 mer HP24.1 Roth Random Primer Kit D for RAPD-PCR 20x 10 mer HP25.1

w

Carl Roth GmbH + Co. KG

Schoemperlenstraße 3-5 • 76185 Karlsruhe P.O. Box 100121 • 76231 Karlsruhe

Phone: +49 (0) 721/ 5606-0 Fax: +49 (0) 721/ 5606-149 [email protected] • www.carlroth.com sse 06/2021

The company is a limited partnership with headquarters in Karlsruhe, reg. court Mannheim HRA 100055. Roth Chemie GmbH, with headquarters in Karlsruhe, reg. court Mannheim HRB 100428, is the personally liable partner. Managing Director: André Houdelet. Sales tax identification number: DE 143621073.

Roth HP22.1 User guide [de, en]