Retsch Cryomill User Manual

Manual

Mixer Mill CryoMill

Translation

© Retsch GmbH, 42781 Haan, Retsch-Allee 1-5, Germany 13.03.2013 0002

Copyright

© Copyright by
Retsch GmbH
Haan, Retsch-Allee 1-5
D-42781 Haan
Federal Republic of Germany

2

1 Notes on the Operating Manual ............................................................................................................ 6

1.1 Explanations of the safety warnings .................................................................................................. 7

1.2 General safety instructions ................................................................................................................ 8

1.3 Repairs ............................................................................................................................................... 9

2 Confirmation ......................................................................................................................................... 10

3 Technical data ....................................................................................................................................... 11

3.1 Protective equipment ....................................................................................................................... 11

3.2 Emissions......................................................................................................................................... 11

3.3 Degree of protection ........................................................................................................................ 12

3.4 Rated power .................................................................................................................................... 12

3.5 Dimensions and weight .................................................................................................................... 12

3.6 Required floor space ........................................................................................................................ 12

4 Transport, scope of delivery, installation .......................................................................................... 13

4.1 Packaging ........................................................................................................................................ 13

4.2 Transport.......................................................................................................................................... 13

4.3 Temperature fluctuations and condensed water ............................................................................. 13

4.4 Conditions for the place of installation ............................................................................................. 13

4.5 Installation of the machine ............................................................................................................... 14

4.6 Removing the transport safeguard .................................................................................................. 14

4.7 Type plate description ...................................................................................................................... 18

4.8 Electrical connection ........................................................................................................................ 19

4.9 Connecting the cooling agent feed .................................................................................................. 19

4.10 Removing the liquid nitrogen feed line ............................................................................................ 23

4.11 Cold air outlet ................................................................................................................................... 24

4.12 Mounting the exhaust hose.............................................................................................................. 24

5 Operating the machine ........................................................................................................................ 26

5.1 Use of the machine for the intended purpose.................................................................................. 26

5.2 Operating elements and displays .................................................................................................... 27

5.3 Summary table of device parts ........................................................................................................ 28

5.4 Operating elements and displays .................................................................................................... 29

5.5 Inserting the grinding jar .................................................................................................................. 31

5.5.1 Inserting and removing CryoMill special grinding jar ................................................................... 31

5.5.2 Inserting the cooling jacket sealing plug ...................................................................................... 34

5.5.3 Insert the adapter for 4/2 x 5 ml grinding jar ................................................................................ 35

5.5.4 Insert the adapter for 6 / 4 / 2 x 2-ml Eppendorf reaction vial ..................................................... 36

5.6 Explanations of the grinding cycles ................................................................................................. 37

5.6.1 Grinding without cooling .............................................................................................................. 37

5.6.2 Grinding with cooling ................................................................................................................... 37

3

5.7 Programs ......................................................................................................................................... 38

5.7.1 Grinding without program ............................................................................................................ 38

5.7.2 Grinding with program ................................................................................................................. 39

5.7.2.1 Selection of a program ......................................................................................................... 39

5.7.2.2 Display of the program grinding parameters ....................................................................... 39

5.7.2.3 Create/change a program .................................................................................................... 39

5.7.2.4 Delete program .................................................................................................................... 40

5.8 Setting grinding cycles ..................................................................................................................... 40

5.9 Setting the precooling time .............................................................................................................. 41

5.9.1 Precooling with definable precooling time ................................................................................... 41

5.9.2 Automatic precooling ................................................................................................................... 41

5.10 Setting the grinding time .................................................................................................................. 42

5.11 Setting the duration of intermediate cooling .................................................................................... 42

5.12 Setting the grinding frequency ......................................................................................................... 43

5.13 Starting the grinding process ........................................................................................................... 43

5.14 Interrupting and continuing the grinding process ............................................................................ 44

5.14.1 Pausing grinding ...................................................................................................................... 44

5.14.2 Cancelling grinding .................................................................................................................. 44

5.15 Stopping the grinding process ......................................................................................................... 44

5.16 Operating hours display ................................................................................................................... 45

5.17 Operating software display .............................................................................................................. 46

5.18 Replacing the machine fuses........................................................................................................... 46

6 Working instructions............................................................................................................................ 48

6.1 General ............................................................................................................................................ 48

6.2 The grinding process ....................................................................................................................... 48

6.3 Quantities of sample material and feed sizes .................................................................................. 48

6.4 Working instructions ........................................................................................................................ 49

7 Safety functions and fault display ...................................................................................................... 50

7.1 Fault messages ............................................................................................................................... 50

8 Cleaning, wear and service ................................................................................................................. 51

8.1 Cleaning ........................................................................................................................................... 51

8.2 Wear ................................................................................................................................................ 51

8.3 Wearing parts .................................................................................................................................. 51

8.4 Service ............................................................................................................................................. 51

8.5 Checks ............................................................................................................................................. 52

9 Safety and occupational health instructions ..................................................................................... 52

9.1 General ............................................................................................................................................ 52

9.2 Safety instructions on the handling of liquid nitrogen ...................................................................... 54

9.3 Oxygen deficiency ........................................................................................................................... 54

4

9.3.1 Dangers ....................................................................................................................................... 54

9.3.2 Causes ......................................................................................................................................... 54

9.3.3 Recommendations ....................................................................................................................... 54

9.3.4 General conduct in the case of an accident ................................................................................ 55

9.4 Cryogenic burns ............................................................................................................................... 55

9.4.1 Dangers ....................................................................................................................................... 55

9.4.2 Causes ......................................................................................................................................... 55

9.4.2.1 Burns through splashes ....................................................................................................... 55

9.4.2.2 Burns through contact .......................................................................................................... 56

9.4.3 Recommendations ....................................................................................................................... 56

9.4.4 General rules of conduct for splashes with liquid nitrogen .......................................................... 56

9.4.4.1 To the eyes .......................................................................................................................... 56

9.4.4.2 On the skin ........................................................................................................................... 56

9.5 Danger of explosion ......................................................................................................................... 56

9.5.1 Dangers ....................................................................................................................................... 56

9.5.2 Causes ......................................................................................................................................... 56

9.5.3 Recommendations ....................................................................................................................... 57

9.5.4 General conduct in the case of an accident ................................................................................ 57

9.6 Oxygen enrichment .......................................................................................................................... 57

9.6.1 Dangers ....................................................................................................................................... 57

9.6.2 Causes ......................................................................................................................................... 57

9.6.3 Recommendations ....................................................................................................................... 57

9.7 Surrounding area of the machine .................................................................................................... 58

9.7.1 Rooms .......................................................................................................................................... 58

10 Disposal ................................................................................................................................................. 59

11 Index ...................................................................................................................................................... 60

Appendix ............................................................................................................................... following pages

5

Notes on the Operating Manual

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1 Notes on the Operating Manual

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This operating manual is a technical guide on how to operate the device safely and
it contains all the information required for the areas specified in the table of
contents. This technical documentation is a reference and instruction manual. The
individual chapters are complete in themselves.

Familiarity (of the respective target groups defined according to area) with the
relevant chapters is a precondition for the safe and appropriate use of the device.

This operating manual does not contain any repair instructions. If faults arise or
repairs are necessary, please contact your supplier or get in touch with Retsch
GmbH directly.

Application technology information relating to samples to be processed is not
included but can be read on the Internet on the respective device’s page at

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www.retsch.com.

Changes

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Subject to technical changes.

Copyright

Disclosure or reproduction of this documentation, use and disclosure of its contents
are only permitted with the express permission of Retsch GmbH.

Infringements will result in damage compensation liability.

6

Notes on the Operating Manual

WARNING

Type of danger / personal injury

Source of danger

– Possible consequences if the dangers are not observed.

• Instructions on how the dangers are to be avoided.

WARNING

CAUTION

Type of danger / personal injury

Source of danger

– Possible consequences if the dangers are not observed.

• Instructions on how the dangers are to be avoided.

CAUTION

NOTICE

Nature of the property damage

Source of property damage

– Possible consequences if the instructions are not observed.

• Instructions on how the dangers are to be avoided.

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1.1 Explanations of the safety warnings

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In this Operating Manual we give you the following safety warnings

Serious injury may result from failing to heed these safety warnings. We give you
the following warnings and corresponding content.

We also use the following signal word box in the text or in the instructions on action
to be taken:

Moderate or mild injury may result from failing to heed these safety warnings.
We give you the following warnings and corresponding content.

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We also use the following signal word box in the text or in the instructions on action
to be taken:

In the event of possible property damage we inform you with the word
“Instructions” and the corresponding content.

We also use the following signal word in the text or in the instructions on action to
be taken:

NOTICE

7

Notes on the Operating Manual

CAUTION

Read the Operating Manual

Non-observance of these operating instructions
– The non-observance of these operating instructions can

result in personal injuries.

• Read the operating manual before using the device.

• We use the adjacent symbol to draw attention to the
necessity of knowing the contents of this operating
manual.

CAUTION

Changes to the machine

– Changes to the machine may lead to personal injury.

• Do not make any change to the machine and use spare parts and

accessories that have been approved by Retsch exclusively.

NOTICE

Changes to the machine

– The conformity declared by Retsch with the European Directives will lose

its validity.

– You lose all warranty claims.

• Do not make any change to the machine and use spare parts and

accessories that have been approved by Retsch exclusively.

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1.2 General safety instructions

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Target group : All persons concerned with the machine in any form
This machine is a modern, high performance product from Retsch GmbH and

complies with the state of the art. Operational safety is given if the machine is
handled for the intended purpose and attention is given to this technical

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documentation.
You, as the owner/managing operator of the machine, must ensure that the people

entrusted with working on the machine:

• have noted and understood all the regulations regarding safety,

• are familiar before starting work with all the operating instructions and

specifications for the target group relevant for them,

• have easy access always to the technical documentation for this machine,

• and that new personnel before starting work on the machine are familiarised

with the safe handling of the machine and its use for its intended purpose,
either by verbal instructions from a competent person and/or by means of

this technical documentation.
Improper operation can result in personal injuries and material damage. You are
responsible for your own safety and that of your employees.
Make sure that no unauthorised person has access to the machine.

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8

Notes on the Operating Manual

The Retsch representative in your country

Your supplier

Retsch GmbH directly

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Reperaturen

1.3 Repairs

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This operating manual does not contain any repair instructions. For your own
safety, repairs may only be carried out by Retsch GmbH or an authorized
representative or by Retsch service engineers.

In that case please inform:

Your Service Address:

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9

Confirmation

I have read and taken note of the contents of all chapters in this operating
manual as well as all safety instructions and warnings.

User

Surname, first name (block letters)

Position in the company

Signature

Service technician or operator

Surname, first name (block letters)

Position in the company

Place, date and signature

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Bestätigung

2 Confirmation

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This operating manual contains essential instructions for operating and maintaining
the device which must be strictly observed. It is essential that they be read by the
operator and by the qualified staff responsible for the device before the device is
commissioned. This operating manual must be available and accessible at the
place of use at all times.

The user of the device herewith confirms to the managing operator (owner) that
(s)he has received sufficient instructions about the operation and maintenance of
the system. The user has received the operating manual, has read and taken note
of its contents and consequently has all the information required for safe operation
and is sufficiently familiar with the device.
As the owner/managing operator you should for your own protection have your
employees confirm that they have received the instructions about the operation of
the machine.

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10

Technical data

Receptacle:

1 50ml steel grinding jar

Grinding component:

1 25mm steel ball each

Feed material:

Quartz gravel particles approx. 4.0 – 6.0mm

Feed quantity:

8ml

Frequency:

30 Hz

Receptacle:

4 5ml steel grinding jar

Grinding component:

2 8mm tungsten carbide balls each

Feed material:

Quartz gravel particles approx. 1.0 – 1.5mm

Feed quantity:

1.5ml

Frequency:

30 Hz

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3 Technical data

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3.1 Protective equipment

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The grinding chamber of the CryoMill laboratory mill is surrounded by a stable
hood.

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3.2 Emissions

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It is only possible to start the device when the hood is closed.

Noise values:
The noise values are also influenced by the properties of the sample material.

No-load operation:

Emission value with regard
to workplace L

Example 1:

Emission value with regard
to workplace L

Operating conditions:

= 61,4 dB(A)

pAeq

= 75,2 dB(A)

pAeq

Example 2:

Emission value with regard
to workplace L

= 66,3 dB(A)

pAeq

Operating conditions:

11

Technical data

CAUTION

Hearing loss

A high sound level may occur depending on the type of material, the
number of balls used, the set grinding frequency and the grinding time.

– Excess noise in terms of intensity and duration can lead to

impairments or permanent damage to hearing.

• Ensure you take suitable soundproofing measures or wear
hearing protection.

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3.3 Degree of protection

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IP30

3.4 Rated power

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260 watts

3.5 Dimensions and weight

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Height : 370 to approx. 630 mm with open hood
Width: 385 mm
Depth: 570 mm

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Weight: approx. 46 kg without grinding jar

3.6 Required floor space

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Supporting surface: 500 mm x 650 mm

12

Transport, scope of delivery, installation

NOTICE

Storage of packaging

– In the event of a complaint or return, your warranty claims may be

endangered if the packaging is inadequate or the machine has not been
secured correctly.

• Please keep the packaging for the duration of the warranty period.

NOTICE

Transport

– Mechanical or electronic components may be damaged.

• The machine may not be knocked, shaken or thrown during

transport.

NOTICE

Temperature fluctuations

The machine may be subject to strong temperature fluctuations during transport
(e.g. aircraft transport)

– The resultant condensed water may damage electronic components.

• Protect the machine from condensed water.

NOTICE

Ambient temperature

– Electronic and mechanical components may be damaged and the

performance data alter to an unknown extent.

• Do not exceed or fall below the permitted temperature range of the
machine (5°C to 40°C / ambient temperature).

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4 Transport, scope of delivery, installation

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4.1 Packaging

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The packaging is designed for the transport route. It corresponds to generally

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4.2 Transport

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acceptable packaging guidelines.

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4.3 Temperature fluctuations and condensed water

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4.4 Conditions for the place of installation

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Atmospheric humidity:
Maximum relative humidity 80% at temperatures up to 31°C, decreasing linearly up

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to 50% relative humidity at 40°C

13

Transport, scope of delivery, installation

NOTICE

Atmospheric humidity

– Electronic and mechanical components may be damaged and the

performance data alter to an unknown extent.

• Do not exceed the admissible range for atmospheric humidity.

NOTICE

Property damage

A very cold vapour plume escapes from the nitrogen gas outlet.
– The vapour plume can greatly cool objects. This is why the humidity in the

air condenses.

• The area above the nitrogen gas outlet (B) up to the ceiling must be
kept free.

NOTE

Installation

– Depending on the operating status of the mill, there may be slight

vibrations.

• Place the mill on an even, flat and balanced supporting surface only.
The supporting surface must be stable and must not vibrate.

NOTICE

Installation of the machine

– It must be possible to disconnet the machine from the mains at any time.

• Install the machine such that the connection for the mains cable is

easily accessible.

NOTICE

Transport safeguard

– Components may be damaged.

• Operate the machine only without the transport safeguard or

transport the machine only with transport safeguard.

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4.5 Installation of the machine

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Installation height: maximum 2000 m above sea level

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4.6 Removing the transport safeguard

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• Place the device on a stable table.

• Pull the device carefully beyond the edge of the table in order to be able to

14

reach the screw (TA).

Transport, scope of delivery, installation

TA

TB

TL1

TL1

Fig. 1: Removing the transport bracket

• Remove the screw (TA) using an Allen key.

Fig. 2:

• Pull the device into the position shown in order to be able to remove
the screw (TB).

• Remove the second screw (TB) and remove the transport bracket
(TL1).

NOTICE

Keep the transport brackets, screws and transport lock for future transport!

15

Transport, scope of delivery, installation

R

F1 U TL2

Fig. 3: Pulling out the condensation collecting filter

• Pull the condensation collecting filter (R) completely out of the device.

• Remove the opening aids (F1) on the side on which you have removed the
transport bracket (TL1).

• Place a soft, clean mat (U) next to the device.

• Tilt the device onto the side on which the transport bracket (TL1)

has been removed.

16

Fig. 4: Tilting the device onto its side

Transport, scope of delivery, installation

TS

TC

TD

TL2

The transport lock (TS), which is labelled with an arrow, is situated on the
underneath of the device.

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Fig. 5: Removing the transport lock

• Loosen and remove the screw (TS).

• Unscrew the two screws (TC) and (TD).

• Remove the transport bracket (TL2).

Fig. 6:

• Re-insert the condensation collecting filter (R).

NOTICE

Keep the transport brackets, screws and transport lock for future transport!

17

Transport, scope of delivery, installation

1 2 3 4 5 6 7 8 9

10

11

12

14

13

GL

Fig. 7: Grip
A grip (GL) is situated underneath both sides of the device

Pos: 5.24.1 /00005 Überschriften/1.1 Ü berschriften/1.1 Überschriften BDA/11 Type nschild Beschreibung @ 3\mod_128093 3953941_9.doc @ 22302 @ 2 @ 1

• Use the grip (GL) to move the device.

4.7 Type plate description

Pos: 5.24.2 /00003 Standard Kapitel/Gen eral Modul Typenschild @ 3\mod_1280 931092443_9.doc @ 22278 @ @ 1

Fig. 8: Type plate lettering
1 Device designation
2 Year of production
3 Part number
4 Serial number
5 Manufacturer’s address
6 CE marking
7 Disposal label
8 Bar code
9 Power version
10 Mains frequency

18

Transport, scope of delivery, installation

WARNING

WARNING

Risk of a fatal electric shock

- An electric shock can cause injuries in the form of burns and cardiac

arrhythmia, respiratory arrest or cardiac arrest.

• Do not clean the blender under running water. Use only a cloth dampened
with water.

• Disconnect the power supply plug before cleaning the blender.

NOTICE

Electrical connection

– Mechanical or electronic components may be damaged.

• Please observe the information on the type plate.

11 Capacity
12 Amperage
13 Number of fuses
14 Fuse type and fuse strength
In the case of questions please provide the device designation (1) or the part

Pos: 5.25 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Elektrisc her Anschluss @ 0\mod_122656506 7445_9.doc @ 3500 @ 2 @ 1

number (3) and the serial number (4) of the device.

4.8 Electrical connection

Pos: 5.26 /00003 Standard Kapitel/Gen eral Modul Elektrischer Anschluss @ 0\ mod_1228918538521_9.doc @ 4731 @ @ 1

When connecting the power cable to the mains supply, use an external fusethat
complies with the regulations applicable to the place of installation .

• Please check the type plate for details on the necessary voltage and
frequency for the device.

• Make sure the levels agree with the existing mains power supply.

• Use the supplied connection cable to connect the device to the mains power

Pos: 5.27 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tra nsport, Lieferumfang und Aufstellen /CryoMil l II Modul externe Absicherung @ 5\mod_1343829054956_9.doc @ 33059 @ @ 1

Pos: 5.28 /00004 Warnhinweise/W0002 WARNUNG Kabelbruch Stromstoß @ 2\mod_1278055276120_9.doc @ 20557 @ @ 1

supply.

The external fuse must be at least T6.3A (230V) T8A (100/120V).

Pos: 5.29 /00004 Warnhinweise/H0008 HIN WEIS Elektrischer Anschluss @ 0\ mod_1228918882424_9.doc @ 4781 @ @ 1

Pos: 5.30 /00005 Überschriften/1.1 Ü berschriften/1.1 Überschriften BDA/11 Kü hlmittelzufluss anschließen @ 0\mod_122690 8410419_9.doc @ 3576 @ @ 1

4.9 Connecting the cooling agent feed

Pos: 5.31 /00004 Warnhinweise/H0062 HIN WEIS Risiko der Eisbildung (CryoMill) @ 4\mod_1314082100670_9.doc @ 25 455 @ @ 1

19

Transport, scope of delivery, installation

CAUTION

Blocked pipe

Ice formation

– Ice or impurities can block pipes and thereby cause malfunctions.

• Keep the cooling system and supply pipe dry and free from

impurities.

• Do not insert the grinding jar if it is damp or covered with frost.

• Insert the cooling jacket seal (KA) if you will not be using the device

for a long period.

• Do not operate the device unattended.

Pos: 5.32 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tra nsport, Lieferumfang und Aufstellen /002 0 CryoMill II Modul Kühlmantel Abdichtung @ 5\mod_1344926871592_9.doc @ 34 120 @ @ 1

20

Transport, scope of delivery, installation

NOTICE

Damage to the machine

Incorrect cooling agent
– The use of cooling agents other than liquid nitrogen will cause the cooling

system to lose its leak tightness.

• Only liquid nitrogen (LN2) may be used as cooling agent with this
device.

KA

Pos: 5.33 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tr ansport, Lieferumfang und Aufstellen /001 8 CryoMill II Modul Schematische Darstell ung des Kühlsystems @ 5\mod_134451 1088330_9.doc @ 33941 @ @ 1

Fig. 9: Cooling jacket seal

Fig. 10: Connecting the cooling agent supply

21

Transport, scope of delivery, installation

CAUTION

The maximum pressure in the external liquid
nitrogen supply line may be a maximum of 1.5

bar.
The use of a safety valve (SV)

is absolutely essential!

B1 (MV)

B2 (TSM)

TSD

TSM

MV

SV

Pos: 5.34 /00010 Bedienungsanleitung en Kapitelsammlungen/CryoMill II/0006 Tr ansport, Lieferumfang und Aufstellen /00 13 CryoMill II Modul Kühlsystem @ 5\ mod_1344511110312_9.doc @ 33952 @ @ 1

Fig. 11: Diagram of the cooling system

Fig. 12: LED displays
The temperature sensor (TSD) in the LN2 outlet controls the solenoid valve (MV).

The LED (B1) lights up when the solenoid valve (MV) is open.
The temperature sensor (TSM), which is downstream of the solenoid valve,

registers whether liquid nitrogen is flowing into the CryoMill. The LED (B2) lights
up when liquid nitrogen flows into the cooling system.

The device must not be moved in a cryogenic state and no additional forces may
act on the cooling agent connection (N). The cooling agent inlet hose must not be
moved while the device is in a cryogenic state.

22

Transport, scope of delivery, installation

CAUTION

Risk of injury and danger of frostbite

Bursting of the inlet hose.
– Depending on the operating state of the device, liquid nitrogen may be

enclosed in the supply line. When heated this may lead to the inlet hose
bursting.

• The maximum pressure in the external liquid nitrogen supply line

may be a maximum of 1.5 bar. The use of a safety valve is absolutely
essential!

CAUTION

Risk of injury and danger of frostbite

Uncontrolled escape of liquid nitrogen
– There is a risk of injury in the event of an uncontrolled escape of liquid

nitrogen.

• Stop the liquid nitrogen supply line immediately!

CAUTION

The connection for the liquid nitrogen (LN2) is a G 1/4 inch threaded connector .
The operating pressure in the liquid nitrogen supply line should be at least 0.5 bar.

The lower the pressure of the liquid nitrogen supply line, the longer the precooling
takes. At 0.5 bar supply line pressure and with the system at room temperature,
precooling requires approx. 10 minutes. Depending on the application, you can
execute up to 4 grinding operations with 10 litres of liquid nitrogen.

Throughout the entire duration of cooling, the air humidity in the chamber
condenses on all cooled parts as a layer of ice .

Pos: 5.35 /00004 Warnhinweise/V0070 VOR SICHT LN2 Austritt (CryoMill) @ 4\ mod_1314082103623_9.doc @ 25491 @ @ 1

Pos: 5.36 /00004 Warnhinweise/H0063 HIN WEIS Wasser aus dem Auffangfilter tro pfen (CryoMill) @ 4\mod_13140821013 89_9.doc @ 25464 @ @ 1

After cooling this condensation drips into the collecting filter and evaporates.

NOTICE

Depending on the ambient temperature, air humidity and duration of use, small

Pos: 5.37 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Kühlmittelz ufluss entfernen @ 1\mod_1238053 128286_9.doc @ 7970 @ @ 1

quantities of water may drip from the collecting filter.

4.10 Removing the liquid nitrogen feed line

Pos: 5.38 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tra nsport, Lieferumfang und Aufstellen /001 2 CryoMill II Modul Kühlmittelzufluss entf ernen @ 5\mod_1344511338025_9.doc @ 33963 @ @ 1

Abb. 13: Removing the liquid nitrogen feed line

Before you remove the liquid nitrogen feed line, the pressure must be relieved for
safety reasons.

23

Transport, scope of delivery, installation

CAUTION

Risk of injury to eyes and skin

Outlet for gaseous nitrogen
– Liquid nitrogen has a temperature of –196 °C and can cause burn-like

injuries or frostbite in the case of contact with the skin or eyes.

• Do not keep any parts of the body in front of the outlet for gaseous
nitrogen (B).

B

B10

B13

Abb. 14: pressure relief

• Press the (B10) pre-cooling and (B13) START keys simultaneously for 2
seconds.

Pos: 5.39 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Kaltluft- Austritt @ 0\mod_1232374121848_9.d oc @ 5510 @ @ 1

4.11 Cold air outlet

Pos: 5.40 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tra nsport, Lieferumfang und Aufstellen /0010 Cr yoMill II Modul Kaltluftaustritt VORSICHT @ 5\mod_1344511373029_9.doc @ 33974 @ @ 1

As long as you keep the keys depressed, the solenoid valve will remain open.

Fig. 15: Outlet for gaseous nitrogen (B)

Pos: 5.41 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Abluftsc hlauch montieren @ 5\mod_134452 2887327_9.doc @ 34030 @ 2 @ 1

4.12 Mounting the exhaust hose

Pos: 5.42 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0006 Tra nsport, Lieferumfang und Aufstellen /0005 Cr yoMill II Modul Abluftschlauch @ 5\ mod_1344519211701_9.doc @ 34020 @ @ 1

24

Transport, scope of delivery, installation

B

BS

BR

Fig. 16: Exhaust hose

Pos: 6.1 /00010 Bedienungsanleitungen Kapitelsammlungen/------- Seitenumbruch ----------- @ 0\mod_1222344373758_ 0.doc @ 2386 @ @ 1

• Fasten the exhaust hose (BR) with the clamp (BS) to the outlet (B).
– The exhaust hose can be extended up to 1 m

25

Operating the machine

NOTICE

Area of use of the machine

– This machine is a laboratory machine designed for 8-hour single-shift

operation.

• This machine may not be used as a production machine nor is it
intended for continuous operation.

Pos: 6.2 /00005 Überschriften/1. Überschri ften/1 Bedienung des Gerätes @ 0\m od_1226565880211_9.doc @ 3519 @ 1 @ 1

5 Operating the machine

Pos: 6.3 /00005 Überschriften/1.1 Überschrif ten/1.1 Überschriften BDA/11 Einsatz d er Maschine bei bestimmungsgemäßer V erwendung @ 0\mod_122647673224 8_9.doc @ 3243 @ 2 @ 1

5.1 Use of the machine for the intended purpose

Pos: 6.4 /00010 Bedienungsanleitungen Kapitelsammlungen/CryoMill II/0007 Bedie nung/0023 CryoMill II Modul Zielgruppe Ma schinentyp @ 5\mod_13438292597 04_9.doc @ 33389 @ @ 1

Target group: operating company, operator

Pos: 6.5 /00010 Bedienungsanleitungen Kapitelsammlungen/CryoMill II/0007 Bedie nung/0007 CryoMill II Modul Einsatz bei besti mmungsgemäße Verwendung Cr yoMill @ 5\mod_1343829239142_9.doc @ 33213 @ @ 1

Name of machine model: CryoMill
This machine is a laboratory device and is suitable for the particularly gentle

grinding and homogenisation of heat-sensitive, soft, fibrous, hard and brittle
materials in a dry and wet state.

The CryoMill is designed exclusively for special grinding jars with screw-top lids (no
standard grinding jars) with steel jacket. It is used for the fast pulverization of up to
six samples simultaneously (dpending on the type of grinding jar).

Available grinding jar volumes :

– 50ml;
– 35ml;
– 25ml;
– 10ml;
– 4 x 5ml including adapter; and

Pos: 6.6 /00004 Warnhinweise/H0007 H INWEIS Einsatzbereich des Gerätes 8 Stün diger @ 1\mod_1236240219096_9.doc @ 7693 @ @ 1

Pos: 6.7 /00010 Bedienungsanleitungen Kapitelsammlungen/------- Seitenumbruch ----------- @ 0\mod_1222344373758_ 0.doc @ 2386 @ @ 1

– 6 x 2ml Eppendorf vials.
The closed grinding system guarantees complete recovery of the samples . Due to

the extremely short grinding time and the high final fineness of the grinding sample,
the CryoMill is also ideally suitable for sample preparation for all spectral analyses.

Final fineness levels of up to 5 µm can be achieved depending on the grinding time
and the specific properties of the sample material.

The optimal grinding jar filling is generally 1/3 of the grinding jar volume.
Voluminous materials such as wool, leaves, grasses and similar are exceptions to
this. A fill level of 70 - 80% is necessary here.

26

Operating the machine

C

F E F A T D FA

R

H

K,L,M

I O U

N P B J S

B

Pos: 6.8 /00005 Überschriften/1.1 Überschrif ten/1.1 Überschriften BDA/11 Grafisch e Ansichten der Bedienelemente und der Anzeige @ 0\mod_1226566362336_9. doc @ 3537 @ @ 1

5.2 Operating elements and displays

Pos: 6.9 /00010 Bedienungsanleitungen Kapitelsammlungen/CryoMill II/0007 Bedie nung/0003 CryoMill II Modul Bedienelemente und Bedienung Gafische Ansichte n @ 5\mod_1343829232365_9.doc @ 33 169 @ 2 @ 1

Fig. 17: Front view

27

Operating the machine

Element

Description

Function

A

Display and control unit:
See below for explanations

Time preselection, frequency preselection and
starting/stopping the machine

B

Nitrogen gas outlet

Outlet for gaseous nitrogen

C

Counterweight left

Counterweight for the cooling jacket with grinding
jar

D

Cooling jacket

Forms the cooling system together with the
grinding jar (T)

E

Hood

Closes the grinding chamber

F

Opening aid

Can be mounted on the grinding jar cover for
simple removal of the grinding jar

FA

Support for opening aid

Storage of the opening aid

H

Fan

Ventilates the motor and the interior of the mill

I

Caution remove mains plug sign

Safety instruction

J

Type plate

Information about the device and connected loads

K

ON / OFF switch

Switches the device on and off

L

Fuse drawer

Accommodates two glass fuses

M

Connector

Connector for the mains lead of the device

N

Cooling agent connection

Connection for liquid nitrogen

O

RS232 interface

Enables updating of the operating software

P

Observe operating instructions sign

Safety instruction

R

Condensation collecting filter

Collects the condensation from the cooling system
and allows it to evaporate

S

Drip tray

Housing protection

T

Grinding jar

Forms the cooling system together with the cooling
jacket (D)

Fig. 18: Rear view

5.3 Summary table of device parts

Pos: 6.10 /00010 Bedienungsanleitungen K apitelsammlungen/------- Seitenumbruch ----------- @ 0\mod_1222344373758 _0.doc @ 2386 @ @ 1

28

Operating the machine

Element

Description

Function

B1

LED on – valve open
LED off – valve closed

Indicates whether the internal solenoid valve on the
liquid nitrogen supply line is open or closed.

B2

LED on – liquid nitrogen is flowing
LED off – no liquid nitrogen

Indicates that liquid nitrogen is flowing into the device.

B3

LED precooling is running (Vkz)

Precooling is taking place / lights up when values are
set

B4

LED grinding is running (Mz)

The sample is ground / lights up when values are set

B5

LED intermediate cooling is running
(Zkz)

Intermediate cooling is taking plate / lights up when
values are set

B7

Frequency minus button

In setting mode and in operating mode:
reduces the set frequency by 1 Hz (1/s). Continuous
pressing switches on fast scrolling of numbers.

Frequency plus button

In setting mode and in operating mode:
increases the set frequency by 1 Hz (1/s). Continuous
pressing switches on fast scrolling of numbers.

B8

Cooling cycle minus button

Reduces the number of total cycles by 1

Cooling cycle plus button

Increases the number of total cycles by 1

B9

Time minus button

In setting mode and in operating mode:
Reducing the set

• Precooling

• Grinding time

• Intermediate cooling time

B10

B7

B8

B15

B2

B1

B11

B12

B13

B9

B14

B3

B4

B5

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5.4 Operating elements and displays

Pos: 6.12 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0002 CryoMill II Modul Anzeige und Bedieneinheit @ 5\mod_1343829230 960_9.doc @ 33158 @ @ 1

Fig. 19: Control unit

29

Operating the machine

Time plus button

In setting mode and in operating mode:
Increasing the set

• Precooling

• Grinding time

• Intermediate cooling time

B10

Setting precooling

Preselection button to set the precooling / switching
automatic precooling on or off)

B11

Setting grinding

Preselection button to set the grinding time

B12

Setting intermediate cooling

Preselection button to set the intermediate cooling time

B13

Start button
Green LED

Starts grinding operation
Indicates grinding operation

Stop button
Red LED and ON

Interrupts or ends grinding operation,
places the machine in stand-by mode

B14

LED - auto precooling

Indicates whether the automatic precooling is switched
on

B15

PROG button

Button to select a program

SET button

Button starts / saves a program editing

Pos: 6.13 /00010 Bedienungsanleitungen K apitelsammlungen/------- Seitenumbruch ----------- @ 0\mod_1222344373758 _0.doc @ 2386 @ @ 1

30

Operating the machine

CAUTION

Danger of injury and frostbite

Escape of liquid nitrogen
– Liquid nitrogen has a temperature of –196 °C and may cause injuries

similar to burns on skin or eye contact or frostbite.

• Never switch the machine on without tightly closed cooling casing.

CAUTION

Crushed or bruised fingers

Falling grinding chamber protective hood
– The protective hood of the grinding chamber can cause crushed or bruised

fingers if it falls down.

• Hold the flap tight when closing.

CAUTION

Clogged piping

Formation of ice
– Ice or foreign matter can clog the pipe and subsequently cause

malfunctioning.

• Do not insert the grinding jar if it is damp or covered with frost.

• Use the cooling jacket sealing plug (KA) if you are not going to use

the device for a long time.

• Do not let the device run without supervision.

Pos: 6.14 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Mahlbe hälter einsetzen @ 0\mod_1226569844 414_9.doc @ 3555 @ @ 1

5.5 Inserting the grinding jar

Pos: 6.15 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill/0101 Warnhin weise/CryoMill VORSICHT Austritt von fl üssigem Stickstoff @ 0\mod_12287496 57712_9.doc @ 4360 @ @ 1

Pos: 6.16 /00004 Warnhinweise/V0008 VOR SICHT Klappe beim Schließen festhalte n (CryoMill) @ 2\mod_127805421350 5_9.doc @ 20549 @ @ 1

Pos: 6.17 /00005 Überschriften/1.1.1 Ü berschriften/111 CryoMill Spezial Mahlbecher einsetzen @ 0\mod_122751681255 5_9.doc @ 3966 @ @ 1

5.5.1 Inserting and removing CryoMill special grinding jar

Pos: 6.18 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill/0101 Warn hinweise/CryoMill HINWEIS Risiko der Eis bildung @ 0\mod_1234165670129_9. doc @ 6140 @ @ 1

Pos: 6.19 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0014 CryoMill II Modul Mahlbecher Ei nsetzen wechseln @ 5\mod_13438 29248165_9.doc @ 33290 @ @ 1

31

Operating the machine

KM

SM

1.

2.

Fig. 20: Dismantling the grinding jar retaining device

• Unscrew the lock nut (KM).

• Remove the screw (SM).

Fig. 21: Inserting the grinding jar

• Insert the special grinding jar filled with the grinding material and grinding
balls into the cooling jacket .

• Twist the grinding jar in completely.

32

Operating the machine

CAUTION

Risk of injury to eyes and skin

The cooling jacket and grinding jar reach very low temperatures
during grinding.

– Risk of injury to eyes and skin from severe frostbite

• As a matter of principle, always wear goggles and

protective gloves when opening the cooling jacket and the
grinding jar.

KM

2.

1.

SM

Fig. 22: Securing the grinding jar

• First tighten the screw (SM) by hand (hand-tight).

• Then tighten the lock nut against the cooling jacket. Use the locking pin

provided to do this.

Ensure that the grinding jar is tightly screwed to the cooling jacket, otherwise liquid
nitrogen can escape.

The lock screw (SM) must be screwed tight and secured with the lock nut so that
no sample material escapes from the grinding jar.

Use the opening aid (F) that is kept on the side of the device to remove the
grinding jar .

• Unscrew the lock nut (KM)

• Remove the screw (SM)

• Mount the opening aid (F) on the grinding jar and open it.

33

Operating the machine

NOTE

1.H0017

Damage to the PTFE grinding jar

– The threading on the grinding jar can become damaged.

• When it is being inserted into the device, the PTFE grinding jar must

be at room temperature and must not be pre-cooled.

NOTICE

Blocked pipe

Ice formation
– Ice or impurities can block pipes and thereby cause malfunctions.

• Insert the cooling jacket seal (KA) as soon as you are no longer using
the device.

F

Pos: 6.20 /00004 Warnhinweise/H0056 HIN WEIS Cryomill PTFE Mahlbecher nur mit Zimmertemperatur einsetzen - Nicht vorkühlen @ 4\mod_1314082879240_9.d oc @ 25509 @ @ 1

Pos: 6.21 /00005 Überschriften/1.1.1 Ü berschriften/111 Kühlmantel Abdichtung eins etzten @ 2\mod_1266314975785_9.doc @ 18787 @ @ 1

Fig. 23: Using the opening aid

5.5.2 Inserting the cooling jacket sealing plug

Pos: 6.22 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0011 CryoMill II Modul Kühlmantela bdichtung einsetzen @ 5\mod_134 3829244950_9.doc @ 33257 @ @ 1

34

Operating the machine

KA

Pos: 6.23 /00005 Überschriften/1.1.1 Ü berschriften/111 Adapter für 4/2 x 5ml Ma hlbecher einsetzen @ 0\mod_1227516748 177_9.doc @ 3960 @ @ 1

Fig. 24: Inserting the cooling jacket seal

5.5.3 Insert the adapter for 4/2 x 5 ml grinding jar

Pos: 6.24 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0001 CryoMill II Modul 5ml Mahlbec her einsetzen @ 5\mod_13438292 29211_9.doc @ 33147 @ @ 1

You can operate the CryoMill with 4 or 2 (5 ml) grinding jars.

• Insert the 5 ml grinding jars into the adapter as shown in the following
diagram.

Fig. 25: Inserting a 5ml grinding jar
Note the positioning of the grinding jars. If you are using two grinding jars, these

must be inserted in opposite openings.

Fig. 26: 5ml grinding jar position

35

Operating the machine

NOTICE

Breaking of Eppendorf reaction vials

Changing material properties
– The Eppendorf reaction vials that are available as accessories change

their material properties during the cryogen grinding.

• Do not reuse reaction vials after the cryogen grinding.

• Do not insert reaction vials in centrifuges after the cryogen grinding

• Grinding may take place with a maximum 25Hz.

Pos: 6.25 /00005 Überschriften/1.1.1 Ü berschriften/111 Adapter für 4/2 x 2 ml Epp endorf Reaktionsgefäße einsetzen @ 1\ mod_1243499921893_9.doc @ 10328 @ @ 1

Fig. 27: Inserting the grinding jar adapter

5.5.4 Insert the adapter for 6 / 4 / 2 x 2-ml Eppendorf reaction vial

Pos: 6.26 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill/0101 Warnhin weise/CryoMill HINWEIS Zerbrechen von Eppendorf Reaktionsgefäßen @ 1\m od_1243499975784_9.doc @ 10336 @ @ 1

Pos: 6.27 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0008 CryoMill II Modul Eppendorf G efäße einsetzen @ 5\mod_134382 9240370_9.doc @ 33224 @ @ 1

You can operate the CryoMill with 2, 4 or 6 Eppendorf reaction vials (2ml).

NOTICE

Grinding with Eppendorf reaction vials may only take place with a maximum 25Hz.
(frequency 1/s)

• Insert the 2ml Eppendorf reaction vials into the adapter for reaction vials as
shown in the diagram below.

Fig. 28: Inserting 2ml Eppendorf reaction vials

36

Operating the machine

Note the positioning of the Eppendorf reaction vials. If you are using two or four
reaction vials, these must be inserted in opposite openings.

Pos: 6.28 /00005 Überschriften/1.1 Übersc hriften/1.1 Überschriften BDA/11 Erläuter ungen zu den Mahlzyklen @ 0\mod_1 227686930552_9.doc @ 4010 @ @ 1

Fig. 29: Position of 2 ml reaction vials

5.6 Explanations of the grinding cycles

Pos: 6.29 /00010 Bedienungsanleitungen K apitelsammlungen/CryoMill II/0007 Bedi enung/0009 CryoMill II Modul Erläuterungen zu den Mahlzyklen @ 5\mod_13438 29242206_9.doc @ 33235 @ 33 @ 1

You can deploy the CryoMill for grinding with cooling or grinding without cooling.

5.6.1 Grinding without cooling

• Switch the CryoMill on at the main switch.

• Set the cryo cycles to - .

• Set the desired grinding time (B9)+(B11).

• Press the START button.

The START LED lights up. The remaining grinding time and the set frequency are
displayed.

5.6.2 Grinding with cooling

The following program points can be set when grinding with cooling.

– precooling (Vkz)
– grinding time (Mz)
– intermediate cooling time (Zkz)
– cryo cycles (Number of cooling/grinding cycles)
– frequency 1/s (Grinding frequency)

A single grinding cycle consists of the precooling and the set grinding time. The
intermediate cooling time does not apply in the case of a single grinding cycle.

37

Operating the machine

B15

Vkz + Mz = total grinding time

Fig. 30: Sequence of a single grinding cycle
Multiple grinding cycles consist of the precooling, the set grinding time and the

intermediate cooling time.
Number of cryo cycles: n

Vkz + (n-1) (Mz + Zkz) + Mz = total grinding time

Precooling + [(number of set cryo cycles) - 1] x (grinding time + intermediate cooling time) +
grinding time = total grinding time

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Fig. 31: Sequence of two grinding cycles

5.7 Programs

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5.7.1 Grinding without program

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Fig. 32: Grinding without program

• Press the PROG button (B15) until “- -” is shown in the memory display .

38

Operating the machine

B15

B7

B8

B9

B10

B11

B12

The display advances one program each time the button is pressed

- - > P1 > P2 > P3 > P4 > P5 > P6 > P7 > P8 > P9

Pos: 6.33 /00005 Überschriften/1.1.1 Ü berschriften/111 Vermahlung mit Program m @ 5\mod_1344590647615_9.doc @ 34 050 @ 3 @ 1

– The SET button (B15) is blocked in the “- -” mode

5.7.2 Grinding with program

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Fig. 33: Grinding with program

5.7.2.1 Selection of a program

• Press the PROG button (B15) until the required program is shown in the

memory display.
– The display advances one program each time the button is pressed .

- - > P1 > P2 > P3 > P4 > P5 > P6 > P7 > P8 > P9

– The buttons B8, B7, B9 are blocked due to the selection of a program.

5.7.2.2 Display of the program grinding parameters
Precooling

• Press the precooling button (B10).
– The set time for precooling appears in the time min (B9) display.

Grinding time

• Press the grinding time button (B11).
– The set time for the grinding time appears in the time min (B9) display.

Intermediate cooling

• Press the intermediate cooling button (B12).
– The set time for the intermediate cooling appears in the time min (B9)

display.

5.7.2.3 Create/change a program

• Press the PROG button (B15) until the required program (memory space) is
displayed in the memory display.

• Press the SET button (B15).
– All displays and the corresponding LEDs for which settings are possible light

up or flash.

39

Operating the machine

B8

• Set the parameters for grinding as described in the following chapters:

- Number of grinding cycles

- Precooling

- Grinding time

- Intermediate cooling time

- Grinding frequency

– It is possible to cancel at any time using the PROG button (B15)

• Press the SET button (B15).
– Pressing the SET BUTTON (B15) again stores all parameters in the

program.

5.7.2.4 Delete program

• Press the PROG button (B15) until the program to be deleted is displayed in
the memory display.

• Press the shortcut SET (B15) and - (B8) for at least 5 seconds.

Fig. 34: Deleting a program
– The program is deleted and the parameters are assigned with the values “0”

or “-”.

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– It is not possible to start a deleted program.

5.8 Setting grinding cycles

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Fig. 35: Setting the grinding cycles

40

• Switch the CryoMill on at the main switch.
– After switching on, the last used grinding parameters are displayed and can

be used.
– You can set 0 ("-") to 9 grinding cycles

NOTICE

If 0 ("-") CryoCycles are set, the grinding is conducted without nitrogen. In this
case the buttons B10, B11 and B12 are blocked.

Operating the machine

B10

B9

ap

– The first cycle consists of the precooling phase and grinding time
– Cycles 2 to 9 each comprise an intermediate cooling and grinding.

• Press the respective buttons (B8) to set the grinding cycles.

• + Pressing briefly increases the number.

• - Pressing briefly reduces the number.

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– The display runs more quickly when pressed longer.

5.9 Setting the precooling time

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NOTICE

The machine runs at a fixed frequency of 5 Hz during the precooling and
intermediate cooling.

A range of from 30 seconds to 99 minutes can be set for precooling.

Fig. 36: Setting precooling
After switching on, the last used grinding parameters are displayed and can be

used.

• Switch the CryoMill on at the main switch.

• Press the button (B10) to set precooling

– The precooling LED lights up.
You can choose between two precooling versions:

5.9.1 Precooling with definable precooling time

• Use the buttons (B9) to set precooling.

• + Pressing briefly increases the duration.

• - Pressing briefly shortens the duration.

The display runs more quickly when pressed longer.

5.9.2 Automatic precooling

In the case of automatic precooling the grinding only starts once the entire system
has been sufficiently cooled. This is checked by a sensor (see chapter
>>Connecting the cooling agent supply <<)

Switch automatic precooling on

• Press the button (B10) for at least 2 seconds.

41

Operating the machine

B12

B9

LED

B11

B9

LED

– The auto precooling (ap) LED is lit up.
– “Auto” is shown in the time min display.

Switch automatic precooling off

• Press the button (B10) for at least 2 seconds.

– The auto precooling (ap) LED is extinguished.

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– “08:00" is shown in the time min display.

5.10 Setting the grinding time

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Fig. 37: Setting the grinding time

• Press the button (B11) to set the grinding time.
The grinding time LED lights up.

• Use the buttons (B9) to set the grinding time

(5 to 30 Hz in 1Hz steps).

– + Pressing briefly increases the duration.

– - Pressing briefly shortens the duration.

The display runs more quickly when pressed longer.

NOTICE

In the case of grinding without cooling, the grinding time can be set directly using

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5.11 Setting the duration of intermediate cooling

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42

the buttons (B9).

Fig. 38: Setting the intermediate cooling

Operating the machine

CAUTION

Danger of injury and frostbite

Escape of liquid nitrogen
– Liquid nitrogen has a temperature of –196 °C and may cause injuries

similar to burns on skin or eye contact or frostbite.

• Never switch the machine on without tightly closed cooling casing.

B7

• Press the button (B12) to set the intermediate cooling .
The intermediate cooling time LED lights up.

• Use the buttons (B9) to set the intermediate cooling time

(30 seconds to 99:00 minutes).

– + Pressing briefly increases the duration.

– - Pressing briefly shortens the duration.

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The display runs more quickly when pressed longer.

5.12 Setting the grinding frequency

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Fig. 39: Frequency 1/s

• Switch the CryoMill on at the main switch.
It is only possible to adjust the grinding frequency if the button (B11) for setting the

grinding parameters has been pressed and the LED (B11) is lit up.

• Press the button (B11).

• Press the respective buttons (B7) to set the grinding frequency .

The selectable frequency range is from 3 to 30 Hz.

• + Pressing briefly increases the frequency.

• - Pressing briefly reduces the frequency.

The display runs more quickly when pressed longer.
The frequency of precooling and intermediate cooling is fixed at 5 Hz. The

frequency cannot be adjusted while setting the precooling or intermediate cooling

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time.

5.13 Starting the grinding process

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NOTICE

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The grinding process can only be started if the hood is closed.

43

Operating the machine

B13

B13

B13

Fig. 40: Starting the grinding process

• Press the START button to start the grinding process.
– The Start LED (green) lights up. At the same time the solenoid valve opens

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and liquid nitrogen can flow into the cooling system.

5.14 Interrupting and continuing the grinding process

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5.14.1 Pausing grinding

Fig. 41: Pausing grinding

• Press the STOP button (B13) once.
– Grinding is interrupted and the LED above the START button flashes.

5.14.2 Cancelling grinding

Fig. 42:

• Press the STOP button (B13) twice.
– The grinding is ended and the two LEDs above the START and STOP

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buttons are off.

5.15 Stopping the grinding process

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44

Operating the machine

K

Fig. 43: Ending the grinding process

• Press the STOP button to end the grinding process.
The STOP LED (red) lights up. At the same time the solenoid valve closes.

In the event that the supply of liquid nitrogen has been interrupted during
operation, this is indicated by the flashing of the remaining time LED (B6) at the
end of the grinding time.

Pressing once interrupts the grinding process, for example to appraise the grinding
sample. The remaining grinding time is still visible on the display.

By pressing the start button again, the mill continues running until the grinding time
has completely expired.

Pressing the stop button twice aborts the grinding process. The machine is now in
start mode.

By pressing the start button, the display is activated again and the grinding time is
reset to the last start setting.

The duration can be changed during the grinding process. The grinding process is
automatically ended on expiry of the grinding time. The display is reset to the last

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value started.

5.16 Operating hours display

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• Switch the device off at the mains switch.

Fig. 44: Switching the device on and off

• Keep the buttons cryo cycles (B8) + and frequency (B7) - pressed
simultaneously and with the buttons pressed, switch the device on at the
mains switch.

45

Operating the machine

K

B7

B8

B7

B8

Fig. 45: Display of the operating hours
The total operating time in hours is displayed. The maximum value that can be

depicted is 999999 hours. The letters bS are displayed in the first two places.
Examples:
bS0 00 012 = 12 hours operating time
bS1 23 456 = 123456 hours operating time

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• Press the STOP button to exit the operating hours display

5.17 Operating software display

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• Switch the device off at the mains switch.

Fig. 46: Switching the device on and off

• Keep the buttons cryo cycles (B8) + and frequency (B7) + pressed
simultaneously and...

• ...with the buttons pressed, switch on the device at the mains switch.

Fig. 47: Display of the software version
The current version of the operating software is displayed across the two right-

hand display windows. The letter S is shown in first place.
Examples:
S 1.23 = Version 1.23 of the operating software
S 2.00 = Version 2.00 of the operating software

• Press the STOP button to exit the display of the

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operating software.

5.18 Replacing the machine fuses

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46

Operating the machine

L

M

Fig. 48: Replacing the device fuses
The following glass fuses are needed for the CryoMill:

2 T4A

• Pull the mains plug from the connector (M).

• Press in the catch at the side. This releases the fuse holder (L) so that it

can be pulled out.

• Replace the fuses.

Pos: 7.1 /00010 Bedienungsanleitungen Kapitelsammlungen/------- Seitenumbruch ----------- @ 0\mod_1222344373758_ 0.doc @ 2386 @ @ 1

• Push the fuse holder (L) in until it engages.

47

Working instructions

CAUTION

2.V0004

Risk of explosion or fire

Changing sample properties
– Consider that the properties and therefore also the hazardousness of your

sample can change during the grinding process.

• Do not use any substances in this device which carry the risk of
explosion or fire.

CAUTION

Danger of personal injury

Dangerous nature of the sample
– Depending on the dangerous nature of your sample, take the

necessary measures to rule out any danger to persons.

• Observe the safety guidelines and datasheets of your
sample material.

Recommended ball filling (unit)

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6 Working instructions

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6.1 General

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The CryoMill is an extremely modern, efficient product from Retsch GmbH.
Due to the large selection of accessories, the CryoMill has a wide range of

potential applications in industrial and research laboratories.
It is primarily deployed in the chemical and pharmaceutical area, in mineralogy and

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biology etc.

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6.2 The grinding process

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• Insert your grinding jar filled with grinding sample and grinding ball/s into the
grinding jar support as described in the chapter "Inserting the grinding jar”.

• Close the hood.

• Set the grinding parameters.

• Open your external liquid nitrogen supply line.

• Start the device.

Do not open the hood during the grinding process.
The grinding jars are brought to a halt immediately by the built-in brake, however

the grinding process can no longer be continued with the remaining grinding time.

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The device must be restarted. The original parameters are available here.

6.3 Quantities of sample material and feed sizes

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48

Working instructions

Grinding jar

nominal

volume

Sample

quantity

Max. feed

size

Ø7 mm

Ø10 mm

Ø12 mm

Ø15 mm

Ø20 mm

Ø25 mm

5.0 ml

0.5 - 2.0 ml

2 mm

1 or 2 - - - - - 10.0 ml

2.0 – 4.0 ml

4 mm

-

1 to 2

1 to 2 - - - 25.0 ml

4.0 – 10.0 ml

6 mm

-

5 or 6

2 to 4

1 or 2 - -

35.0 ml

6.0 - 15.0 ml

6 mm

-

6 to 9

4 to 6

2 or 3 1 -

50.0 ml

8.0 - 20.0 ml

8 mm

-

12 to 14

6 to 8

3 or 4 1 1

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Arbeitsweise

6.4 Working instructions

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The grinding jar and its content are cooled by means of liquid nitrogen (LN2) until it
is possible to grind soft materials such as rubber.

Precooling is required until the ideal grinding temperature is reached. The drive
operates at a lower frequency (5Hz) here to prevent the moving parts of the device
from freezing. The fan is switched on during precooling and blows the air heated
by the motor in the front through the openings of the rocker arms into the grinding
chamber. This warm air prevents the rocker arm bearing from cooling down. It is
therefore also necessary to allow the fan to run on for a while after switching the
grinding off.

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In the preliminary tests an average precooling of approx. 10 to 15 minutes was
determined. The grinding time was approx. 4 – 5 minutes.

The valve is switched off when the optimal temperature is reached. Grinding can
begin as from this time and the valve will be switched on and off according to the
temperature.

Grinding can also take place without cooling, in which case the cryo cycles are set
to -.

Manual cooling is started by pressing the shortcut (B10) and (START B13). The
valve remains open as long as the buttons are kept pressed.

49

Safety functions and fault display

rror
cod

(FEHLER) BESCHREIBUNG

DEFECT DESCRIPTION

TRANSLATION

E10

ANTRIEB ÜBERLASTET

DRIVE OVERLOAD

E21

FEHLER DREHZAHL

FAILURE ROTATION SPEED

E22

FEHLER TASTATUR

FAILURE KEYPAD

E24

FEHLER VENTIL

FAILURE VALVE

E41

FEHLER DREHZAHLSENSOR

FAILURE SPEED SENSOR

E42

FEHLER TEMPERATURSENSOR 1

FAILURE TEMPERATURE

SENSOR 1

E43

FEHLER TEMPERATURSENSOR 2

FAILURE TEMPERATURE

SENSOR 2

E51

FEHLER SICHERHEITSSCHALTER

SATETY SWITCH DEFECTIVE

E85

KEIN STICKSTOFF

NO LIQUID NITROGEN

E86

LECKAGE

LEAKAGE

H40

MASCHINE SCHLIESSEN

CLOSE MACHINE

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7 Safety functions and fault display

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7.1 Fault messages

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50

Cleaning, wear and service

CAUTION

Personal injury

Incorrect repairs

– This operating manual does not include instructions for repair.

• For your own safety repairs should be carried out only by Retsch

GmbH or an authorised representative (service technicians).

R

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8 Cleaning, wear and service

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8.1 Cleaning

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Fig. 49: Pulling out the condensation collecting filter

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8.2 Wear

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8.3 Wearing parts

Pos: 9.8 /00004 Warnhinweise/V0013 VORS ICHT Reparaturen @ 0\mod_123244 3088492_9.doc @ 5530 @ @ 1

The condensation collecting filter must be cleaned regularly depending on the
ambient conditions, the degree of use and the general air humidity.

• Pull out the condensation collecting filter (R).

• Clean the condensation collecting filter under running water with a little

standard washing up liquid.

• Allow the collecting filter to dry and then insert in the device again.

The grinding tools may become worn, depending on the frequency of the grinding
operation and the sample material. The grinding jars and the balls should be
regularly checked for wear and replaced if necessary.

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8.4 Service

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51

Safety and occupational health instructions

CAUTION

Danger of injury to eyes and skin

Frostbites through liquid nitrogen
– Liquid nitrogen has a temperature of –196 °C and may cause

injuries similar to burns on skin or eye contact or cause frostbite.

• Always use goggles and wear protective gloves when
opening the cooling casing and the grinding jar.

CAUTION

Use of liquid nitrogen

– Retsch GmbH rules out any liability claims that may arise in the

use of liquid nitrogen or similar cooling agents.

• Please observe the safety regulations of the cooling liquid
supplier.

CAUTION

Handling liquid nitrogen

General hazardous situations
– The following hazardous situations may arise in the handling of liquid

The grinding tools may become worn, depending on the frequency of the grinding
operation and the sample material. The grinding jars and the balls should be

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regularly checked for wear and replaced if necessary.

8.5 Checks

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Correct functioning of the hood switch must be checked regularly.

• Switch on the machine with switch (K).

• Start grinding operation with start button (B13 - Start).

• Lift the hood.

If there is an opening gap of a few cm the machine switches itself off and F04
appears in the display.

• Cancel F04 with stop button (B13 - Stop).
If this switching-off function does not take place the CryoMill must be checked

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immediately by Retsch-Service.

9 Safety and occupational health instructions

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9.1 General

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This section summarises the general occupational health and safety guidelines for
the handling of liquid nitrogen.

52

nitrogen: oxygen deficiency situations, cryogenic burns, danger of
explosion, oxygen enrichment

• All users must be informed about the dangers of working with liquid
nitrogen in order to be able to work safely.

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Safety and occupational health instructions

53

Safety and occupational health instructions

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9.2 Safety instructions on the handling of liquid nitrogen

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9.3 Oxygen deficiency

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The main components of air according to volumes are as follows:

– Oxygen O2 21 %
– Nitrogen N2 78 %
– Argon Ar 1 %

The gases contained in the atmosphere are not toxic. However, a change in
concentration (in particular changes to the oxygen concentration) may have effects
on life and burns. It is therefore essential for the air breathed in to contain sufficient
oxygen (> 19 %).

Humans cannot detect changes in the composition of air within the time that will

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9.3.1 Dangers

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actually be necessary because the components are colourless and odourless.

A danger of suffocation exists as a result of the normal evaporation of the liquid
nitrogen that pushes out the oxygen in the air. An oxygen deficiency is dangerous
and can cause death through suffocation. The reaction of the organism to oxygen
deficiency will differ greatly depending on the individual. It is not possible to provide
precise and generally applicable information on the symptoms of an oxygen
deficiency.

Example: under normal conditions (20° C; 1013 mbars) 1 l liquid nitrogen

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evaporates to produce 680 l nitrogen gas.

9.3.2 Causes

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An oxygen deficiency may arise during the following work or in the following
conditions:

– Nitrogen as liquid or gas
– Natural evaporation of liquid nitrogen
– Refilling of liquid nitrogen
– Leaks in containers for liquid or gaseous nitrogen
– Defect in the air feed or outlet
– Tipping over of the container

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This list is not complete.

9.3.3 Recommendations

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In order to prevent the danger of an oxygen deficiency, the following measures
must be taken.

The vessel:

• must be kept in a vertical position.

54

• must be provided with a suitable insulating lid.

• must be protected from direct sunlight and may not be set up near heat

sources.

Safety and occupational health instructions

• may not be transported in vehicles in filled state.

• must be protected from impact, knocks and rapid movements.

• Ventilate all installation rooms constantly and appropriately.

• Wear protective clothing (suitable gloves, goggles or face protection and

safety shoes).

• Check the oxygen content of the room constantly.

• Always carry an oxygen metre.

• Only trained personnel may work with liquid nitrogen.

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This list is not complete.

9.3.4 General conduct in the case of an accident

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In the case of an accident from oxygen deficiency the following regulations should
be observed.

• Secure the surrounding area to avoid any subsequent accidents.

• Act quickly.

• The rescuers must take measures to protect themselves (respiratory

protection device).

• Move the injured persons from the hazardous area.

• Observe the company instructions for emergencies.

• Ventilate the rooms affected sufficiently.

• Investigate the cause of the accident.

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This list is not complete.

9.4 Cryogenic burns

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Liquid nitrogen is very cold (-196° C).
The surfaces of vessels that were in contact in liquid nitrogen (in particular during

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9.4.1 Dangers

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the filling process) may cause skin burns on contact.

Cryogenic liquids may:

– bring about burns to the human body
– make specific materials (metal and plastic) that are not suitable for low

temperatures brittle

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– generate strong misting depending on atmospheric humidity

9.4.2 Causes

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There are two types of cryogenic burns:

9.4.2.1 Burns through splashes

When handling samples and in general when handling liquid nitrogen, personnel
must protect themselves from splashes. They can cause cryogenic burns with
serious consequential damage, in particular to eyes and face.

55

Safety and occupational health instructions

9.4.2.2 Burns through contact

Contact of the skin with cold material causes frostbite or cryogenic burns.
The interior of vessels or the samples may never be touched or held with bare

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9.4.3 Recommendations

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hands.

In order to prevent the danger of burning the following points must be observed:

• Never bring cryogenic liquids in contact with the skin.

• Never touch the cold non-isolated or iced walls of a vessel.

• Wear personal protective equipment (suitable gloves, goggles or face

protection and safety shoes).

• Keep the vessel upright.

• Use suitable material (e.g. metal hose or PTFE hose) to refill.

• Train personnel.

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This list is not complete.

9.4.4 General rules of conduct for splashes with liquid nitrogen

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9.4.4.1 To the eyes

• Rinse eyes with much water for 15 min.

• Follow the company instructions for emergencies.

• Consult a doctor

9.4.4.2 On the skin

• Do not rub.

• If possible remove or loosen clothing.

• Slowly and gradually warm the parts affected.

• Do not apply anything to the burnt area.

• Follow the company instructions for emergencies.

• Consult a doctor.

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Both lists are not complete.

9.5 Danger of explosion

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9.5.1 Dangers

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9.5.2 Causes

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The evaporation of liquid nitrogen may lead to an overpressure in the vessel.

An increase in the vessel may be attributable to:

56

– incorrect set-up (use of a closable lid)
– icing on the neck and on the insulating lid

Safety and occupational health instructions

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This list is not complete.

9.5.3 Recommendations

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To avoid the danger of explosion:

• Always use suitable insulating lids (pay attention to waste gas opening).

• Observe filling levels to avoid the formation of ice on the insulating lid.

• Set up the vessel in dry and roofed rooms.

• Monitor atmospheric humidity in the installation room.

• Check vessel regularly for the collection of condensed water.

• Check vessel regularly for damage to surface or material damage

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This list is not complete.

9.5.4 General conduct in the case of an accident

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In the case of an accident from oxygen deficiency the following regulations should
be observed.

• Secure the surrounding area to avoid any subsequent accidents.

• Act quickly.

• The rescuers must take measures to protect themselves (respiratory

• Move the injured persons from the hazardous area.

• Observe the company instructions for emergencies.

• Ventilate the rooms affected sufficiently.

• Investigate the cause of the accident.

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This list is not complete.

9.6 Oxygen enrichment

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9.6.1 Dangers

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9.6.2 Causes

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9.6.3 Recommendations

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Oxygen enrichment may increase the danger of explosion and fire.

Oxygen may be condensed from the air if liquid nitrogen is used and similarly be
liquefied because the boiling point of oxygen (approx. -183° C) is above that of
nitrogen (-196° C).

The following points must be considered in the case of oxygen enrichment:

protection device).

• Do not smoke.

• Where possible, keep easily inflammable materials away from the vessel.

57

Safety and occupational health instructions

• Remove all sources of fire (naked flame and light, spark producers, matches,
lighters etc.).

• Ventilate installation rooms constantly and appropriately.

• Clean floor regularly.

• Train personnel.

• Wear personal protective equipment.

• Check oxygen content constantly.

• Always carry an oxygen metre.

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This list is not complete

9.7 Surrounding area of the machine

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9.7.1 Rooms

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The room in which the machine is located must:

• permit operation without danger to the employees

• have a constantly running and appropriate ventilation system

• have a level and non-porous floor that is also able to bear the load of the

vessel

• have safety datasheets on liquid nitrogen visible for all to see

• prevent unauthorised access

• permit the safe filling of the vessel

• permit accessibility to the vessel for inspection, cleaning and maintenance

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This list is not complete.

58

Disposal

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10 Disposal

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Please observe the respective statutory requirements with respect to disposal.
Information on disposal of electrical and electronic machines in the European

Community.
Within the European Community the disposal of electrically operated devices is

regulated by national provisions that are based on the EU Directive 2002/96/EC on
Waste Electrical and Electronic Equipment (WEEE).

Accordingly, all machines supplied after 13.08.2005 in the business-to-business
area to which this product is classified, may no longer be disposed of with
municipal or household waste. To document this they have the following label:

=== Ende der Liste für Textmarke Inhalt ===

Fig. 50: Disposal label
Since the disposal regulations within the EU may differ from country to country we

would request you to consult your supplier.

59

11 Index

4

4 A ....................................................................... 47

5

5ml grinding jar position ...................................... 35

A

Amperage ........................................................... 19

Assign/change memory space ........................... 39

Atmospheric humidity ......................................... 13

Automatic precooling .......................................... 41

B

Bar code ............................................................. 18

bS ........................................................................ 46

C

Capacity .............................................................. 19

Causes ............................................. 54, 55, 56, 57

CE marking ......................................................... 18

D

Danger of explosion ........................................... 56

Dangers ........................................... 54, 55, 56, 57

Degree of protection .......................................... 12

Delete program .................................................. 40

Depth.................................................................. 12

Device designation ............................................. 18

Diagram of the cooling system .......................... 22

Dimensions and weight ...................................... 12

Dismantling the grinding jar retaining device ..... 32

Display of the grinding parameters .................... 39

Display of the operating hours ........................... 46

Display of the software version .......................... 46

Disposal ............................................................. 59

Disposal label ..................................................... 18

Disposal label ..................................................... 59

E

Changes ............................................................... 6

Checks ................................................................ 52

Clean................................................................... 51

Cleaning .............................................................. 51

Cleaning, wear and service ................................ 51

Cold air outlet ...................................................... 24

Condensation ...................................................... 23

Condensation collecting filter ........................ 16, 51

Conditions for the place of installation ................ 13

Confirmation ....................................................... 10

Connecting the cooling agent feed ..................... 19

Connection cable ................................................ 19

Cooling jacket ..................................................... 32

Cooling jacket seal .............................................. 21

Copyright .............................................................. 6

Create/change a program ................................... 39

cryo cycles .......................................................... 37

Cryogenic burns .................................................. 55

Electrical connection .......................................... 19

Emission value with regard to workplace.......... 11

Emissions ........................................................... 11

Ending the grinding process .............................. 45

Eppendorf .......................................................... 36

Eppendorf reaction vial ...................................... 36

Exhaust hose ............................................... 24, 25

Explanations of the grinding cycles ................... 37

Explanations of the safety warnings .................... 7

External fuse ...................................................... 19

F

Fault messages .................................................. 50

Fill level .............................................................. 26

Final fineness levels ........................................... 26

Flashing of the remaining time LED ................... 45

frequency 1/s ..................................................... 37

Frequency range ................................................ 43

Front view .......................................................... 27

60

Function of the hood switch ................................ 52

Fuse holder ......................................................... 47

Fuse strength ...................................................... 19

Fuse type ............................................................ 19

G

G 1/4 inch ........................................................... 23

General ......................................................... 48, 52

General conduct in the case of an accident . 55, 57
General rules of conduct for splashes with liquid

nitrogen ........................................................... 56

General safety instructions ................................... 8

Glass fuses ......................................................... 47

Grinding cycles ................................................... 41

Grinding frequency ............................................. 43

Grinding jar retaining device ............................... 32

Grinding jar volumes ........................................... 26

Grinding time ...................................................... 42

Intermediate cooling........................................... 43

Intermediate cooling time ................................... 37

Interrupting and continuing the grinding process44

L

Laboratory mill ................................................... 11

Layer of ice ........................................................ 23

Liquid nitrogen supply interrupted ...................... 45

L

.................................................................... 11

pAeq

M

Mains frequency ................................................. 18

Manufacturer’s address ..................................... 18

Materials ............................................................ 26

Maximum relative humidity ................................ 13

Moderate or mild injury ........................................ 7

N

Name of machine model .................................... 26

Grinding time ...................................................... 37

Grinding with cooling .......................................... 37

Grinding with program ........................................ 39

Grinding without cooling ..................................... 37

Grinding without program ................................... 38

Grip ..................................................................... 18

H

Height.................................................................. 12

Hood ................................................................... 48

I

Insert the adapter for 4/2 x 5 ml grinding jar ....... 35

Inserting a 5ml grinding jar ................................. 35

Inserting and removing CryoMill special

grinding jar .................................................... 31

Inserting reaction vials ........................................ 36

Inserting the cooling jacket seal ......................... 35

Inserting the cooling jacket sealing plug ............. 34

Noise values ...................................................... 11

Notes on the Operating Manual ........................... 6

Number of fuses ................................................. 19

O

Opening gap ...................................................... 52

Operating elements and displays ...................... 29

Operating elements and displays ...................... 27

Operating hours ................................................. 46

Operating hours display ..................................... 45

Operating hours display ..................................... 46

Operating software display ............................. 46

Operating the machine....................................... 26

Operating time ................................................... 46

Optimal grinding jar filling .................................. 26

Original parameters ........................................... 48

Outlet for gaseous nitrogen ............................... 24

Oxygen deficiency .............................................. 54

Inserting the grinding jar ..................................... 31

Inserting the grinding jar ..................................... 32

Inserting the grinding jar adapter ........................ 36

Installation height ................................................ 14

Installation of the machine .................................. 14

Oxygen enrichment ............................................ 57

P

Packaging .......................................................... 13

Part number ....................................................... 18

61

Power version ..................................................... 18

Precooling ........................................................... 41

Precooling ........................................................... 37

Precooling with definable precooling time .......... 41

pressure relief ..................................................... 24

pressure relieve .................................................. 23

Programs ............................................................ 38

property damage ................................................... 7

Protective equipment .......................................... 11

Pulling out the condensation collecting filter ...... 16

Q

Quantities of sample material and feed sizes 48

R

Rated power ....................................................... 12

reaction vial ......................................................... 36

Rear view ............................................................ 28

Recommendations ..................................54, 56, 57

Recovery of the samples .................................... 26

Regulations for the place of installation .............. 19

Remaining grinding time ..................................... 48

Remove the grinding jar...................................... 33

Removing the liquid nitrogen feed line ............... 23

Removing the liquid nitrogen feed line ............... 23

Removing the transport safeguard ..................... 14

Repairs.................................................................. 9

Replacing the machine fuses .......................... 46

Required floor space ........................................... 12

Rooms................................................................. 58

S

Safety and occupational health instructions ....... 52

Safety functions and fault display ....................... 50

Safety instructions on the handling of liquid

nitrogen ........................................................... 54

Safety warnings .................................................... 7

Seal ..................................................................... 21

Serial number ..................................................... 18

serious injury ........................................................ 7

Service ............................................................... 51

Service Address ................................................... 9

Setting grinding cycles ................................... 40

Setting precooling .............................................. 41

Setting the duration of intermediate cooling 42

Setting the grinding cycles ................................. 40

Setting the grinding frequency ....................... 43

Setting the grinding time .................................... 42

Setting the precooling time ............................ 41

Single grinding cycle .......................................... 37

Software ............................................................. 46

Special grinding jars ........................................... 26

Starting the grinding process ............................. 43

Starting the grinding process ............................. 44

Stopping the grinding process ........................... 44

Summary table of device parts .......................... 28

Supply line pressure .......................................... 23

Surrounding of the machine ............................... 58

T

T 4 A................................................................... 47

Target group ...................................................... 26

Target group ........................................................ 8

Technical data .................................................... 11

Temperature fluctuation and condensed water . 13

The grinding process ...................................... 48

threaded connector ............................................ 23

Total operating time ........................................... 46

Transport ............................................................ 13

Transport, scope of delivery, installation ........... 13

Type plate .......................................................... 19

type plate description ......................................... 18

Type plate lettering ............................................ 18

U

Securing the grinding jar ..................................... 33

Selection of a program ........................................ 39

Sequence of a single grinding cycle ................... 38

Sequence of two grinding cycles ........................ 38

62

Use of the machine for the intended purpose .... 26

Using the opening aid ........................................ 34

V

vapour plume ...................................................... 14

Version of the operating software ....................... 46

Voluminous materials ......................................... 26

W

Wear ............................................................. 51, 52

Wearing parts ................................................... 51

Weight ................................................................ 12

Width .................................................................. 12

Working instructions ........................................... 48

Working instructions ........................................... 49

Y

Year of production .............................................. 18

63

Authorized person for the compilation of technical documents:

J. Bunke (technical documentation)

The following records are held by Retsch GmbH in the form of Technical Documentation:

Detailed records of engineering development, construction plans, study (analysis) of the measures required for
conformity assurance, analysis of the residual risks involved and operating instructions in due form according to
the approved regulations for preparation of user information data.

The CE-conformity of the Retsch Mixer Mill Type CryoMill is assured herewith.

In case of a modification to the machine not previously agreed with us as well as the use of not
licensed spare parts and accessories this certificate will lose its validity.

Retsch GmbH Haan, january 2010
Dr. Stefan Mähler

Manager technical services

Ret sch GmbH  Retsch-Alle e 1-5  427 81 Haan  Germa n y  w w w .retsch .com

Certificate of CE-Conformity according to:
EC Mechanical Engineering Directive 2006/42/EC

Applied harmonized standards, in particular:
DIN EN ISO 12100 Security of machines

EC Directive Electromagnetic Compatibility 2004/108/EC
Applied standards, in particular:

EN 61000-3-2/-3 Electromagnetic compatibility (EMC)
EN 61236 Electrical measuring, operating, controlling and laboratory equipment – EMC-

requirements in conjunction with EN 61000

EN 55011 Limit values and measuring procedures for noise suppression of industrial, scientific

and medical high frequency devices

Additional applied standards, in particular

DIN EN 61010-1 Safety prescriptions concerning measuring-, operating-, controlling- and

laboratory equipment

Mixer MILL

CryoMill

FB-EW-805-063 (E) Änderungsstand C 01/2012

CERTIFICATE OF CE-CONFORMITY

Translation

Copyright

® Copyright by
Retsch GmbH
Haan, Retsch-Allee 1-5
D-42781 Haan
Federal Republic of Germany