Panasonic MCO-20AIC User Manual

MCO-20AIC

Large Capacity Cell Culture CO2 Incubator

Features:

• The industry’s most complete cell culture solution for highly regulated applications or conventional incubation.

• Patented SafeCell UV system decontaminates the chamber air without downtime

Panasonic… the new name for SANYO

www.panasonic.com/biomedical

Key Features & Beneﬁts

The Panasonic Large Capacity Cell Culture CO2 incubator has the capacity and ﬂexibility to grow with your culturing needs while providing a precise and repeatable environment.

SafeCell UV Decontamination

(U.S. Patent 6255103)

The patented SafeCell UV system scrubs interior airﬂow to destroy airborne and humidity pan contaminants.

CO2 Control

Panasonic proprietary single-beam (IR) CO

sensor delivers precise CO2 control,

quick recovery following door openings, and auto sampling with no moving parts. Ceramic-based infrared system is maintenance free, and continuous zero calibration is standard.

InCu-saFe® Copper Stainless Steel Alloy

InCu-saFe® copper-enriched stainless steel alloy combines the corrosion resistance and durability of stainless steel with the germicidal properties of copper. The inCu-saFe

walls and shelves signiﬁcantly reduce the risk of contamination developing on internal surfaces.

SafeCell UV

Active Background

Contamination Control

(5 min. door openings)

PREP: 15 min.

Before sterilization by UV lamp, remove all attachments

SafeCell UV 24hr

Decontamination Protocol

(24 Hours, total)

(trays, tray supports, side supports, top duct, rear duct, humidifying pan, and pan cover with fan) in the chamber.

Ideal for Highly Regulated or Sensitive Applications

The combination of Panasonic’s CO2 incubator performance functions permit use with conﬁdence in high-value cell protocols among hard-to-grow cell lines, cells highly sensitive to contamination, ultra-sensitive media and reagents, or protocols that require a strict isolation and sterilization between processes.

Active Background Contamination Control

At the base of the plenum, an isolated beam of high intensity, ozone-free UV light destroys contaminants in the air and in the water pan, away from cell cultures, not simply collected in a HEPA ﬁlter. Even small particles, which can easily pass through a HEPA ﬁlter, are destroyed.

Plenum components isolate UV light to protect cell cultures, while the UV process continues in the background as programmed without downtime.

Airborne contaminants are eliminated by an automatic 5 minute UV cycle (programmable 0 - 30 minutes). Trace contaminants which attach to walls, shelves and plenum components are

destroyed by the germicidal properties of the inCu-SaFe

copper-enriched stainless

steel surfaces.

Other design factors which help mitigate contamination include condensation control, inner door gasket design and triple 0.3 micron ﬁlters for vent air and CO

sensor sampling.

• The SafeCell UV air ﬂow plenum promotes temperature uniformity through the chamber, shaped by natural and mechanical convection through and around the perforated shelves with gentle circulation through the plenum for UV sterilization and warm water humidiﬁcation.

• Contaminants contained within the distilled water in the humidity pan are destroyed by UV.

• Sterile, humidiﬁed air is released from the lower plenum for vertical convection through and around the perforated shelves

• Interior air motion stops when the door is opened, minimizing movement of room air into the chamber.

Start Incubation

Start Cycle: 24 hours

Follow keypad function code. UV lamp is on for 24 hours.

SafeCell UV on: 5 min. after door opening

Scrubs interior air ﬂow to destroy airborne and humidity pan contaminants.

SafeCell UV on: 5 min. after door opening

Elapsed Time:

Conventional High Heat

Decontamination

(24 Hours, total)

15 min. 30 min. 45 min. 1 2

PREP: 15 min.

Remove interior components sensitive to high heat.

Start Cycle: 90 min.

Interior chamber elevates to high heat.

2 Large Capacity Cell Culture CO2 Incubator www.panasonic.com/biomedical

Sterilize: 14 hours

Interior chamber remains at high heat.

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Contamination Control

UV Decontamination vs. High Heat Decontamination

Independent testing1 conﬁrms that the UV decontamination technique employed by the Panasonic incubator is equally effective against contamination as conventional high heat decontamination over a range of +90°C to +140°C.

Whenever overnight or event decontamination of the Panasonic incubator chamber is desired, all interior components are removed for autoclaving, exposing all interior surfaces to ultraviolet light.

During normal operation when cells are being incubated within the chamber, the UV lamp is visibly isolated from the cell culture chamber by a plenum cover over the humidity pan, permitting UV decontamination of circulated, humidiﬁed air and humidity pan surface water to remain in process without damaging the cells.

A Comparative Analysis of Ultraviolet Light Decontamination vs. High Heat Sterilization in the Cell Culture CO2 Incubator, with the Use of Copper-Enriched Stainless Steel Construction to Achieve Active Background Contamination Control" H. Busujima; D. Mistry 2007

UV vs. High Heat Comparison

High Heat

Method Panasonic UV

Test Results, Maximum Log Reductions

Bacteria > 4.5 > 4.5 > 4.5

Yeast > 2.9 > 2.9 > 2.9

Mold > 2.7 > 2.7 > 2.7

Decontamination Options

Overnight

Active Background Contamination Control

  



+140°C +90°C

 

Mycoplasma Survival Results

Mycoplasma Strain

Mycoplasma fermentans PG18

Mycoplasma orale CH19299

Mycoplasma arginini G230

Mycoplasma hominis PG21

Positive

Control

survival no survival no survival survival

Conventional Type

304 Stainless Steel

Panasonic

InCu-saFe

Conventional Copper C1100

SafeCell UV on: 5 min. after door opening

Chart summarizes test results with four strains of mycoplasma. Results demonstrate how Panasonic inCu-saFe

copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel. Detailed test results are available from Panasonic.

SafeCell UV on: 5 min. (every 12 hours)

Finish.

Shelves, humidity pan and plenum are returned to operating position.

Finish.

Incubator must cool from high heat temperatures to near ambient.

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