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MCO-20AIC
Large Capacity
Cell Culture
CO2 Incubator
Features:
• The industry’s most complete cell culture
solution for highly regulated applications or
conventional incubation.
• Patented SafeCell UV system decontaminates
the chamber air without downtime
Panasonic… the new name for SANYO
www.panasonic.com/biomedical
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Key Features & Benefits
The Panasonic Large Capacity Cell Culture CO2 incubator has the capacity and flexibility to grow with your culturing needs while providing a
precise and repeatable environment.
SafeCell UV
Decontamination
(U.S. Patent 6255103)
The patented SafeCell UV system scrubs
interior airflow to destroy airborne and
humidity pan contaminants.
CO2 Control
Panasonic proprietary single-beam (IR)
CO
sensor delivers precise CO2 control,
2
quick recovery following door openings,
and auto sampling with no moving
parts. Ceramic-based infrared system is
maintenance free, and continuous zero
calibration is standard.
InCu-saFe® Copper
Stainless Steel Alloy
InCu-saFe® copper-enriched stainless
steel alloy combines the corrosion
resistance and durability of stainless
steel with the germicidal properties
of copper. The inCu-saFe
®
walls and
shelves significantly reduce the risk of
contamination developing on internal
surfaces.
SafeCell UV
Active Background
Contamination Control
(5 min. door openings)
PREP: 15 min.
Before sterilization by UV lamp, remove all attachments
SafeCell UV 24hr
Decontamination Protocol
(24 Hours, total)
(trays, tray supports, side supports, top duct, rear duct,
humidifying pan, and pan cover with fan) in the chamber.
Ideal for Highly Regulated or
Sensitive Applications
The combination of Panasonic’s CO2
incubator performance functions permit
use with confidence in high-value cell
protocols among hard-to-grow cell lines,
cells highly sensitive to contamination,
ultra-sensitive media and reagents, or
protocols that require a strict isolation and
sterilization between processes.
Active Background
Contamination Control
At the base of the plenum, an isolated
beam of high intensity, ozone-free UV
light destroys contaminants in the air and
in the water pan, away from cell cultures,
not simply collected in a HEPA filter. Even
small particles, which can easily pass
through a HEPA filter, are destroyed.
Plenum components isolate UV light
to protect cell cultures, while the UV
process continues in the background as
programmed without downtime.
Airborne contaminants are eliminated
by an automatic 5 minute UV cycle
(programmable 0 - 30 minutes). Trace
contaminants which attach to walls,
shelves and plenum components are
destroyed by the germicidal properties of
the inCu-SaFe
®
copper-enriched stainless
steel surfaces.
Other design factors which help mitigate
contamination include condensation
control, inner door gasket design and
triple 0.3 micron filters for vent air and
CO
sensor sampling.
2
• The SafeCell UV air flow plenum
promotes temperature uniformity
through the chamber, shaped by
natural and mechanical convection
through and around the perforated
shelves with gentle circulation through
the plenum for UV sterilization and
warm water humidification.
• Contaminants contained within the
distilled water in the humidity pan are
destroyed by UV.
• Sterile, humidified air is released from
the lower plenum for vertical convection through and around the perforated
shelves
• Interior air motion stops when the door is
opened, minimizing movement of room
air into the chamber.
Start Incubation
Start Cycle: 24 hours
Follow keypad function code.
UV lamp is on for 24 hours.
SafeCell UV on:
5 min. after door
opening
Scrubs interior air
flow to destroy
airborne and
humidity pan
contaminants.
SafeCell UV on:
5 min. after door
opening
SafeCell UV on:
5 min. after door
opening
Elapsed Time:
Conventional High Heat
Decontamination
(24 Hours, total)
15 min. 30 min. 45 min. 1 2
PREP: 15 min.
Remove interior components
sensitive to high heat.
Start Cycle: 90 min.
Interior chamber elevates to high heat.
2 Large Capacity Cell Culture CO2 Incubator www.panasonic.com/biomedical
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Sterilize: 14 hours
Interior chamber remains at high heat.
4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
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Contamination Control
UV Decontamination vs.
High Heat Decontamination
Independent testing1 confirms that the
UV decontamination technique employed
by the Panasonic incubator is equally
effective against contamination as
conventional high heat decontamination
over a range of +90°C to +140°C.
Whenever overnight or event
decontamination of the Panasonic
incubator chamber is desired, all interior
components are removed for autoclaving,
exposing all interior surfaces to ultraviolet
light.
During normal operation when cells are
being incubated within the chamber,
the UV lamp is visibly isolated from the
cell culture chamber by a plenum cover
over the humidity pan, permitting UV
decontamination of circulated, humidified
air and humidity pan surface water to
remain in process without damaging the
cells.
1
A Comparative Analysis of Ultraviolet Light Decontamination vs.
High Heat Sterilization in the Cell Culture CO2 Incubator, with the Use
of Copper-Enriched Stainless Steel Construction to Achieve Active
Background Contamination Control" H. Busujima; D. Mistry 2007
UV vs. High Heat Comparison
High Heat
Method Panasonic UV
Test Results, Maximum Log Reductions
Bacteria > 4.5 > 4.5 > 4.5
Yeast > 2.9 > 2.9 > 2.9
Mold > 2.7 > 2.7 > 2.7
Decontamination Options
Overnight
Active Background
Contamination Control
+140°C +90°C
Mycoplasma Survival Results
Mycoplasma
Strain
Mycoplasma
fermentans PG18
Mycoplasma
orale CH19299
Mycoplasma
arginini G230
Mycoplasma
hominis PG21
Positive
Control
survival no survival no survival survival
survival no survival no survival survival
survival no survival no survival survival
survival no survival no survival survival
Conventional Type
304 Stainless Steel
Panasonic
InCu-saFe
®
Conventional
Copper C1100
SafeCell UV on:
5 min. after door
opening
Chart summarizes test results with four strains of mycoplasma. Results demonstrate how Panasonic inCu-saFe
®
copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining
both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel.
Detailed test results are available from Panasonic.
SafeCell UV on: 5 min.
(every 12 hours)
Finish.
Shelves, humidity pan and plenum
are returned to operating position.
Finish.
Incubator must cool from high heat temperatures to near ambient.
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Contamination Control
Ozone ReleasePanasonic Lamp Germicidal Effect Eye Sensitivity Sunlight
SafeCell UV System
MCO-20AIC includes SafeCell UV
contamination control technology based
on an integrated combination of narrow
bandwidth, ozone-free ultraviolet light,
ceramic infrared CO
copper-enriched stainless steel alloy
interiors and Direct Heat and Air Jacket
heating managed by a microprocessor
controller.
These incubators offer the industry’s
most stable cell culture environment
and are useful for the most critical
applications where continuous
contamination control is essential to
cell viability.
Panasonic SafeCell UV Series CO
incubators offer significant economic
benefits by minimizing interruptions
for decontamination, by improving cell
culture growth and expression under
stable, repeatable conditions, and by
minimizing the potential for product loss.
control, inCu-SaFe®
2
2
SafeCell UV Lamp Program Cycles
Mode Function
After H2O
Vaporization
After Door
Opening
OFF If UV protection is not desired.
24 Hour
Continuous ON
The UV lamp automatically cycles ON for up to 90 minutes following the ten -minute H
2
vapor cycle, reducing the H
onto a naturally cooler section of the interior oor for easy wipe-up.
UV lamp automatically ON for ve minutes af ter door is closed decontaminates
incoming room air.
Useful for overnight decontamination prior to rst use, clinical decontamination protocols
between patients, or following total chamber wipe -out after maintenance or ser vice.
to water droplets. These droplets automatically condense
2O2
2O2
• SafeCell UV includes a programmable
ultraviolet lamp, isolated from cell
cultures, that sterilizes conditioned air
and humidity reservoir water to prevent
contamination without affecting cell
cultures in vitro.
• SafeCell UV inhibits the growth of mycoplasma, bacteria, molds, spores, yeasts
and fungi without costly HEPA filter air
scrubbers which accumulate contaminants in the chamber air.
The SafeCell UV lamp cycle is factory set for normal use, and can be re-programmed as desired by entering parameters
through the central microprocessor control panel.
• High temperature decontamination systems, which can actually encourage in vitro growth of heat resistant thermophilic
and hyperthermophilic microorganisms,
are avoided.
Eliminates Need for HEPA Filter
and Heat Cycle
The MCO-20AIC inhibits the growth of
mycoplasmas, bacteria, molds, spores,
yeast and fungi without costly HEPA filter
air scrubbers which accumulate contami-
Humidifying Water Comparison
nants in the chamber, or disruptive, high
temperature decontamination schemes
which can actually encourage growth of
heat resistant thermophilic and hyperther-
mophilic microorganisms in vitro.
No exposure to UV
Test results after three months confirm the
efficacy of Panasonic SafeCell UV protection
on humidifying water after three months.
Five-minute exposure to
SafeCell UV
As a result, the MCO-20AIC offers a sen-
sitive yet robust platform for short term,
high-throughput drug discovery projects
as well as intermediate and long-term cell
culture investigations.
4 Large Capacity Cell Culture CO2 Incubator www.panasonic.com/biomedical
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Control System
Control and Monitoring
• Control and information center includes
central mounted touchpad and digital
display.
• Precise PID logic controls adjust to
all temperature setpoints and alarm
parameters.
Microprocessor Based Controller
Panasonic expertise in electronic
innovations applies to the Panasonic
MCO-20AIC microprocessor control
system. All incubator functions are
managed by a fully integrated controller
which acquires and processes information
from data entry, setpoints and alarm
parameters.
• P.I.D. Proportional, integral and derivative controls supervise temperature, and
other features for accurate, repeatable
performance.
• A range of setpoint, alarm and programmable inputs are established through the
use of function keys.
• Standard parameters are factory-set for
quick start-up, and all parameters may be
changed as required.
• A remote alarm terminal mounted at the
rear of the cabinet can be connected to
an external alarm system.
The MCO-20AIC control panel is center
mounted in the outer door for easy access, even when incubators are stacked.
Microprocessor based controls manage
all incubator functions including setpoints,
alarm parameters, UV lamp periods, programming, calibration and diagnostics.
Extra-large digital displays are easy to
read. Tactile feedback touchpad data shift
and entry keys simplify operation. When
stacked, door mounted controls remain
easily accessible at eye level.
Cabinet Design
• High volume, low profile cabinets are
stackable with field-reversible doors.
• Interior components and adjustable
shelves are configured for easy access.
• Digital Temperature Display
• Heat ON Lamp
MCO-20AIC
Interior components and adjustable shelves
are configured for easy access, in situ sterilization and flexible arrangement for a variety
of applications.
Model MCO-20AIC is designed for use with
a variety of standard cell culture vessels
and protocols. Four adjustable shelves are
included, standard; maximum shelf capacity
is 15 shelves.
• UV Lamp
• Door Open Lamp
• Low Water Lamp
• Overtemperature
Warning Lamp
• Overtemperature Setpoint Adjustment
• CO
Inject Lamp
2
• Digital CO
Display Multi-function
2
• Gas Cylinder Switch Key (optional)
• Audible Alarm
Silence Key
• Gas Cylinder Supply
Indicator (optional)
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Design & Technology
Direct Heat and Air Jacket Heating System
Zone Location Function Energy Microprocessor Controller
Main
(Red)
Base
(Yel lo w)
Side, top and rear walls Dominant heat source Variable
Floor
Base heater elevates the humidity reservoir
to achieve 95% RH at 37 °C
Variable
Energizes any, all or
in combination of heating
elements as required.
Front
(Green)
Air Jacket
(White)
Insulation
(Gray)
Outer door
Side, top and rear walls
Side, top and rear walls, door
Direct Heat and Air Jacket
Heating System
The patented Direct Heat and Air Jacket
heating system surrounds the inner walls
with a natural convection airow that
converts to radiant wall heat through
thermal conduction to achieve accurate,
uniform and highly responsive temperature control within the chamber.
• The microprocessor controller directs
proportional distribution of power to
independent heating sources surrounding the chamber.
• Arranged in three zones, these sources
include the side, top and rear walls, the
chamber base and the outer door.
• Each zone is controlled by the microprocessor which manages continuous
feedback from the chamber sensors.
Warms the inner glass in response to ambient conditions;
eliminates condensation on glass and around the opening
and promotes temperature uniformity
Sealed. Surrounds interior chamber with natural
air convection and promotes chamber wall uniformity
Promotes energy efciency, mitigates effect of
ambient temperature uctuations on air jacket
The patented Direct Heat and Air Jacket heating system distributes proportional energy to the interior chamber
through a natural convection air jacket surrounded by a high density insulation to protect against ambient temperature
fluctuations
Infrared CO2 Control System
The Panasonic MCO-20AIC uses a
unique ceramic based infrared sensor
system to maintain precise CO
regardless of temperature and relative
humidity changes within the incubator
chamber. Sensor stability is especially
useful following door openings while
temperature and humidity return
to equilibrium.
The ceramic based sensor is maintenance
free with no moving parts and eliminates
control
2
Variable
– –
– –
Ergonomic Cabinet Design
With reversible inner and outer doors, a
single Panasonic incubator offers the industry’s most exible installation options
available.
• Low prole cabinet with door-mounted
control panel permits easy access and
viewing.
• The outer door latch and door heater
cable is easily switched if a reverse
opening is required.
filament bulbs or electro-mechanical
“chopping” devices.
• The CO
every four hours.
• The system allows CO
sensor automatically calibrates
2
control over
2
MCO-20AIC
a range from 0-20% in 0.1% setpoint
increments.
• Actual CO
is displayed on the main
2
control panel.
• A CO
sample port mounted on the incu-
2
bator front permits convenient confirmation of chamber CO
density.
2
MCO-20IC shown with double stack option.
6 Large Capacity Cell Culture CO2 Incubator www.panasonic.com/biomedical
Page 7
Product Specifications
MCO-20AIC Specifications
Description
Model Number MCO-20AIC- PA
Dimensions
Interior
w x f-b x h
Exterior
w x f-b x h
Volume
Shelves
Net Weight
Major Operating Systems
SafeCell UV System Standard
Sensor Standard
IR CO
2
InCu-SaFe
®
Copper Enriched Stainless Steel Interior Standard
Controller/Display, Door Mounted Standard
Direct Heat, Air (DHA) Air Jacket Standard
24.4” x 20.6” x 26.2”
620 x 523 x 6 65 mm
30.3” x 27.9” x 35.4”
770 x 709 x 899 mm
7.6 cu.ft.
215 L
205 lbs
93 kg
Decontamination
Interior UV Lamp, Programmable, Ozone Free Standard
Copper Enriched Stainless Steel Interior
with Germicidal Protection
Standard
Energy, Elecrical & Utilities
Maximum Power Consumption 310W
Maximum Heat Discharge 1062 BTU
Electrical 115V,60Hz with NEM A 5-15 plug provided; requires NEMA 5 -15R grounded receptacle
CO
Gas Connection 4 to 6mm inner diameter tubing
2
CO
Gas Pressure Nominal 4.3 PSI from two- stage CO2 regulator
2
CO
Gas Cylinder Swichover System Optional
2
Environmental Performance
Temperature Control Range +5ºC above ambient to 50°C (in a 5ºC to 35º C ambient )
Temperature Control Uniformity Deviation ±0.25º C (in 25º C ambient, setting 37 ºC, 5% CO2, no load)
CO
Control Range and Deviation 0% to 20%, ±0.15% in 25ºC ambient, setting 37ºC, 5% CO2, no load
2
CO
Sensor Platform
2
Calibration Single point zero automatic
CO
2
Ceramic based, single beam, dual wavelength measurement
of actual vs. contrast, with continuous auto-zero calibration.
Airow Gentle vertic al airow, continuous with inner door closed.
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Page 8
Product Specifications & Accessories
628
34
44469
20
880
665
80
523
“dŒ¹ƒR?[ƒh?oŒûˆÊ’u
125
125
15
15
620
730
770
MCO-20AIC Specifications
Control, Monitoring, Alarm
Temperature and CO2 Control P.I.D., setpoint resolution 0.1% and 0.1°C
Display LED digital display messaging
Data Acquisition Data Acquisition Automatic log function of temperature and CO
Communications
Catalo g #MCO -420MA, remote alarm contacts standard. Optional 4-20 mA connection.
Optional PC interface, Catalog No. MTR -480 with RS232/RS485 data ports available.
Cabinet Design and Construction
Superstructure, Exterior Cabinet and Door Galvanized steel exterior, baked-on enamel nish
Interior and Shelves Copper-enriched stainless steel
Inner Door Tempered glass
Insulation Rigid foam polyurethane
Outer Door Reversible, heated
Access Port Single opening with interior and exterior 30mm non-VOC silicone stoppers
Leveling Feet 4, adjustable
Optional Accessories
Automatic CO
CO
Cylinder Regulator, CGA tting 320 MCO-10 0L
2
Cylinder Switchover System MCO- 21GC- PW
2
Roller Base. For use in single or stacked installations MCO-20RB-PW
InCu-SaFe
Includes two shelf brackets, Full shelf
Communications Por t. Located at rear of chamber
Connector, cable and software not supplied
Communications Por t. Located at rear of chamber,
analog 4 -20mA.
SafeCell UV System Kit Narrow- bandwidth
253.7nm lamp and assembly
®
Shelf and Brackets
MCO -58S T-P W
MTR-480-PW
MCO-420MA-PW
Built-In
.
2
Panasonic Biomedical products include a broad range of accessories to meet specific applications requirements.
For accessory requirements or options not listed herein, contact Panasonic or your authorized Panasonic sales representative.
Panasonic Healthcare Company of North America
1300 Michael Drive, Suite A, Wood Dale, IL 60191
Toll Free USA (800) 858-8442, Fax (630) 238-0074
www.panasonic.com/biomedical
© Panasonic Printed in USA 2012.03 JK
JB201205V2
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