Ocean Optics USB2000 Plus Installation And Operation Manual

1.

USB2000+ Fiber Optic Spectrometer
Installation and Operation Manual

Document Number 270-00000-000-02-0108

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

Additional
Offices:

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
PRC. 200051

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics B.V. (Europe)

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Copyright © 2001-2007 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hire d out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or register ed service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.

Table of Contents

About This Manual .......................................................................................................... iii

Document Purpose and Intended Audience.............................................................................. iii

Document Summary.................................................................................................................. iii

Product-Related Documentation ............................................................................................... iii

Upgrades......................................................................................................................... iv

Service ............................................................................................................................ iv

Chapter 1: Introduction ......................................................................1

Product Overview ............................................................................................................ 1

Features .......................................................................................................................... 2

System Requirements ..................................................................................................... 2

EEPROM Utilization ..................................................................................................................2

About SpectraSuite.................................................................................................................... 2

Sampling System Overview....................................................................................................... 3

How Sampling Works............................................................................................................ 3

Interface Options ............................................................................................................. 3

Shipment Components.................................................................................................... 3

Additional Equipment Needed......................................................................................... 4

Other Accessories Available ........................................................................................... 4

Chapter 2: Installing the USB2000+...................................................6

Overview ......................................................................................................................... 6

USB2000+ Installation..................................................................................................... 6

USB Mode ................................................................................................................................. 6

Serial Port Mode........................................................................................................................ 7

Connect Spectroscopic Accessories ............................................................................... 7

USB2000+ Configuration................................................................................................. 7

Configuring the USB2000+ in SpectraSuite ..............................................................................7

Configuring the USB2000+ in OOIBase32................................................................................ 8

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Table of Contents

Chapter 3: Troubleshooting...............................................................9

Overview ......................................................................................................................... 9

USB2000+ Connected to Computer Prior to SpectraSuite Installation ........................... 9

Windows Operating Systems ....................................................................................................9

Remove the Unknown Device from Windows Device Manager ........................................... 9

Remove Improperly Installed Files........................................................................................ 10

Mac Operating Systems ............................................................................................................ 10

Linux Operating Systems ..........................................................................................................11

Appendix A: Calibrating the Wavelength of the USB2000+............13

Overview ......................................................................................................................... 13

About Wavelength Calibration......................................................................................... 13

Calibrating the Spectrometer........................................................................................... 14

Preparing for Calibration............................................................................................................ 14

Calibrating the Wavelength of the Spectrometer ...................................................................... 14

Saving the New Calibration Coefficients: USB Mode ...................................................... 16

Appendix B: External Triggering .......................................................17

Overview ......................................................................................................................... 17

Normal (Free Running).............................................................................................................. 17

External Software Trigger.......................................................................................................... 17

External Hardware Trigger ........................................................................................................17

External Synchronization Trigger ..............................................................................................18

Appendix C: Specifications................................................................19

Overview ......................................................................................................................... 19

How the USB2000+ Works.............................................................................................. 19

USB2000+ Components Table.................................................................................................. 20

USB2000+ Specifications................................................................................................ 21

20-Pin Accessory Connector Pinout................................................................................ 22

20-Pin Accessory Connector Pinout Diagram........................................................................... 22

20-Pin Accessory Connector – Pin Definitions and Descriptions.............................................. 22

Index .....................................................................................................25

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About This Manual

Document Purpose and Intended Audience

This document provides the users of USB2000+ Spectrometers with instructions for setting up,
calibrating and performing experiments with their spectrometer.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: Installing the

USB2000+

Chapter 3: Troubleshooting

Appendix A: Calibrating the

Wavelength of the USB2000+

Appendix B: External Triggering

Appendix C: Specifications

Contains descriptive information about the USB2000+
Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.

Provides installation instructions.

Contains recommended steps to isolate and correct common
problems.

Provides instructions for calibrating the USB2000+
Spectrometer.

Contains information about external triggering for the
USB2000+.

Contains technical specifications and connector pinouts for the
USB2000+ Spectrometer.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com

document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.

. Select Technical → Operating Instructions, then choose the appropriate

• Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/SpectraSuite.pdf

• Detailed instructions for the Breakout Box are located at:

http://www.oceanoptics.com/technical/HR4_breakout.pdf

• Detailed instructions for external triggering are located at:

http://www.oceanoptics.com/technical/external-triggering.pdf

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.

.

.

About This Manual

Engineering-level documentation is located on our website at Technical → Engineering Docs.
You can also access operating instructions for Ocean Optics products from the Software and Technical

Resources CD that ships with the product.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

Service

Our Total Technical Service protection plans protect your spectrometer for one year for any contingency -

- no matter what the circumstances, including total loss. Under these plans, Ocean Optics will repair or
replace your instrument with no deductible or any other out-of-pocket expense required from you. USB
service plans include the following:

• TTS-USB: Protects your Ocean Optics USB spectrometer for any contingency for 1 year.

Provides for full spectrometer replacement, if necessary. Limited to 1 full replacement per year.

• TTS-USB-E: This special plan for Ocean Optics USB spectrometers not previously covered

protects for any contingency for 1 year. Provides for full spectrometer replacement, if necessary.

Limited to 1 full replacement per year. This coverage starts 30 days after purchasing the plan.

In addition, we also have the WAVECAL-USB spectrometer maintenance and calibration
service available to recalibrate, clean and perform any adjustments necessary on your USB2000+
Spectrometer.
Contact us to learn more about these great service plans.

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Chapter 1

Introduction

Product Overview

The USB2000+ Miniature Fiber Optic Spectrometer is a unique combination of technologies a powerful
2-MHz analog-to-digital (A/D) converter, programmable electronics, a 2048-element CCD-array detector,
and a high-speed USB 2.0 port. This innovative combination produces our fastest spectrometer yet and
provides resolution to 0.35 nm (FWHM). The USB2000+ allows users to capture and store a full
spectrum into memory every millisecond (that's 1,000 full spectra every second) when the spectrometer is
interfaced to a computer via a USB 2.0 port. The USB2000+ is perfect for chemical, biochemical and
other applications where fast reactions need to be monitored.

The USB2000+ Spectrometer connects to a computer via the USB port or serial port. When connected
through a USB 2.0 or 1.1, the spectrometer draws power from the host computer, eliminating the need for
an external power supply. The USB2000+, like all USB devices, can be controlled by our SpectraSuite
software, a completely modular, Java-based spectroscopy software platform that operates on Windows,
Macintosh and Linux operating systems.

Ocean Optics USB2000+ Fiber Optic Spectrometer Typical Set-up

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1: Introduction

Features

 Sony ILX511 2048-element linear silicon CCD array detector
 Responsive from 200 to 1100 nm
 Sensitivity of up to 75 photons/count at 400 nm; 41 photons/count at 600 nm
 An optical resolution of ~10.0 nm (FWHM)
 Integration times from 3 ms to 65 seconds
 EEPROM storage for

• Wavelength Calibration Coefficients

• Linearity Correction Coefficients

• Absolute Irradiance Calibration (optional)

 Low power consumption of only 250 mA @ 5 VDC
 16 bit, 3MHz A/D Converter
 5 triggering modes
 20-pin connector for interfacing to external products
 CE Certification
 RoHS compliant

System Requirements

You can use the USB2000+’s USB connectivity with any computer that meets the following
requirements:

• Operating system is one of the following:

• Windows – 98/Me/2000/XP

• Apple Macintosh – OS X version 10.0 or later

• Linux – Red Hat 9 or later, Fedora (any version), Debian 3.1 (Sarge), and SUSE (9.0 or

later)

• Ocean Optics’ SpectraSuite software application installed and configured for use.

EEPROM Utilization

An EEPROM memory chip in each USB2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The SpectraSuite software
application reads these values directly from the spectrometer, enabling the ability to “hot-swap”
spectrometers between computers without entering the spectrometer coefficients manually on each
computer.

About SpectraSuite

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

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1: Introduction

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation

).

Sampling System Overview

How Sampling Works

Ocean Optics components function in a sampling system as follows:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light from the light source transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.

6. The spectrometer passes the sample information to SpectraSuite.

7. SpectraSuite compares the sample to the reference measurement and displays processed spectral
information.

Interface Options

The USB2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a computer via a USB or serial port. However, you must create custom
software if using the serial port. SpectraSuite software is available if you are connecting via the USB port.

Shipment Components

The following information and documentation ships with the USB2000+ Spectrometer:

 Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.

 USB Cable (USB-CBL-1)

Use this cable to connect your spectrometer to a computer running on a Windows, Mac or Linux
operating system.

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1: Introduction

 Wavelength Calibration Data Sheet

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. SpectraSuite Operating Software reads this calibration data from
your spectrometer when it interfaces to a computer via the USB port.

Note

Please save the Wavelength Calibration Data Sheet for future reference.

 Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and
spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these
files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources
CD.

Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the Software and Technical
Resources CD package.

Additional Equipment Needed

 SpectraSuite Operating Software

See About SpectraSuite

for more information.

Other Accessories Available

Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.

 Fibers
 Light Sources
 Integrated Sampling Systems
 Cuvettes
 Filter Holders

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