Ocean Optics USB2000 Plus Installation And Operation Manual

1.

USB2000+ Fiber Optic Spectrometer
Installation and Operation Manual

Document Number 270-00000-000-02-0108

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

Additional
Offices:

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
PRC. 200051

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics B.V. (Europe)

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Copyright © 2001-2007 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hire d out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or register ed service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.

Table of Contents

About This Manual .......................................................................................................... iii

Document Purpose and Intended Audience.............................................................................. iii

Document Summary.................................................................................................................. iii

Product-Related Documentation ............................................................................................... iii

Upgrades......................................................................................................................... iv

Service ............................................................................................................................ iv

Chapter 1: Introduction ......................................................................1

Product Overview ............................................................................................................ 1

Features .......................................................................................................................... 2

System Requirements ..................................................................................................... 2

EEPROM Utilization ..................................................................................................................2

About SpectraSuite.................................................................................................................... 2

Sampling System Overview....................................................................................................... 3

How Sampling Works............................................................................................................ 3

Interface Options ............................................................................................................. 3

Shipment Components.................................................................................................... 3

Additional Equipment Needed......................................................................................... 4

Other Accessories Available ........................................................................................... 4

Chapter 2: Installing the USB2000+...................................................6

Overview ......................................................................................................................... 6

USB2000+ Installation..................................................................................................... 6

USB Mode ................................................................................................................................. 6

Serial Port Mode........................................................................................................................ 7

Connect Spectroscopic Accessories ............................................................................... 7

USB2000+ Configuration................................................................................................. 7

Configuring the USB2000+ in SpectraSuite ..............................................................................7

Configuring the USB2000+ in OOIBase32................................................................................ 8

270-00000-000-02-0108 i

Table of Contents

Chapter 3: Troubleshooting...............................................................9

Overview ......................................................................................................................... 9

USB2000+ Connected to Computer Prior to SpectraSuite Installation ........................... 9

Windows Operating Systems ....................................................................................................9

Remove the Unknown Device from Windows Device Manager ........................................... 9

Remove Improperly Installed Files........................................................................................ 10

Mac Operating Systems ............................................................................................................ 10

Linux Operating Systems ..........................................................................................................11

Appendix A: Calibrating the Wavelength of the USB2000+............13

Overview ......................................................................................................................... 13

About Wavelength Calibration......................................................................................... 13

Calibrating the Spectrometer........................................................................................... 14

Preparing for Calibration............................................................................................................ 14

Calibrating the Wavelength of the Spectrometer ...................................................................... 14

Saving the New Calibration Coefficients: USB Mode ...................................................... 16

Appendix B: External Triggering .......................................................17

Overview ......................................................................................................................... 17

Normal (Free Running).............................................................................................................. 17

External Software Trigger.......................................................................................................... 17

External Hardware Trigger ........................................................................................................17

External Synchronization Trigger ..............................................................................................18

Appendix C: Specifications................................................................19

Overview ......................................................................................................................... 19

How the USB2000+ Works.............................................................................................. 19

USB2000+ Components Table.................................................................................................. 20

USB2000+ Specifications................................................................................................ 21

20-Pin Accessory Connector Pinout................................................................................ 22

20-Pin Accessory Connector Pinout Diagram........................................................................... 22

20-Pin Accessory Connector – Pin Definitions and Descriptions.............................................. 22

Index .....................................................................................................25

ii 270-00000-000-02-0108

About This Manual

Document Purpose and Intended Audience

This document provides the users of USB2000+ Spectrometers with instructions for setting up,
calibrating and performing experiments with their spectrometer.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: Installing the

USB2000+

Chapter 3: Troubleshooting

Appendix A: Calibrating the

Wavelength of the USB2000+

Appendix B: External Triggering

Appendix C: Specifications

Contains descriptive information about the USB2000+
Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.

Provides installation instructions.

Contains recommended steps to isolate and correct common
problems.

Provides instructions for calibrating the USB2000+
Spectrometer.

Contains information about external triggering for the
USB2000+.

Contains technical specifications and connector pinouts for the
USB2000+ Spectrometer.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com

document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.

. Select Technical → Operating Instructions, then choose the appropriate

• Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/SpectraSuite.pdf

• Detailed instructions for the Breakout Box are located at:

http://www.oceanoptics.com/technical/HR4_breakout.pdf

• Detailed instructions for external triggering are located at:

http://www.oceanoptics.com/technical/external-triggering.pdf

270-00000-000-02-0108 iii

.

.

.

About This Manual

Engineering-level documentation is located on our website at Technical → Engineering Docs.
You can also access operating instructions for Ocean Optics products from the Software and Technical

Resources CD that ships with the product.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

Service

Our Total Technical Service protection plans protect your spectrometer for one year for any contingency -

- no matter what the circumstances, including total loss. Under these plans, Ocean Optics will repair or
replace your instrument with no deductible or any other out-of-pocket expense required from you. USB
service plans include the following:

• TTS-USB: Protects your Ocean Optics USB spectrometer for any contingency for 1 year.

Provides for full spectrometer replacement, if necessary. Limited to 1 full replacement per year.

• TTS-USB-E: This special plan for Ocean Optics USB spectrometers not previously covered

protects for any contingency for 1 year. Provides for full spectrometer replacement, if necessary.

Limited to 1 full replacement per year. This coverage starts 30 days after purchasing the plan.

In addition, we also have the WAVECAL-USB spectrometer maintenance and calibration
service available to recalibrate, clean and perform any adjustments necessary on your USB2000+
Spectrometer.
Contact us to learn more about these great service plans.

iv 270-00000-000-02-0108

Chapter 1

Introduction

Product Overview

The USB2000+ Miniature Fiber Optic Spectrometer is a unique combination of technologies a powerful
2-MHz analog-to-digital (A/D) converter, programmable electronics, a 2048-element CCD-array detector,
and a high-speed USB 2.0 port. This innovative combination produces our fastest spectrometer yet and
provides resolution to 0.35 nm (FWHM). The USB2000+ allows users to capture and store a full
spectrum into memory every millisecond (that's 1,000 full spectra every second) when the spectrometer is
interfaced to a computer via a USB 2.0 port. The USB2000+ is perfect for chemical, biochemical and
other applications where fast reactions need to be monitored.

The USB2000+ Spectrometer connects to a computer via the USB port or serial port. When connected
through a USB 2.0 or 1.1, the spectrometer draws power from the host computer, eliminating the need for
an external power supply. The USB2000+, like all USB devices, can be controlled by our SpectraSuite
software, a completely modular, Java-based spectroscopy software platform that operates on Windows,
Macintosh and Linux operating systems.

Ocean Optics USB2000+ Fiber Optic Spectrometer Typical Set-up

270-00000-000-02-0108 1

1: Introduction

Features

 Sony ILX511 2048-element linear silicon CCD array detector
 Responsive from 200 to 1100 nm
 Sensitivity of up to 75 photons/count at 400 nm; 41 photons/count at 600 nm
 An optical resolution of ~10.0 nm (FWHM)
 Integration times from 3 ms to 65 seconds
 EEPROM storage for

• Wavelength Calibration Coefficients

• Linearity Correction Coefficients

• Absolute Irradiance Calibration (optional)

 Low power consumption of only 250 mA @ 5 VDC
 16 bit, 3MHz A/D Converter
 5 triggering modes
 20-pin connector for interfacing to external products
 CE Certification
 RoHS compliant

System Requirements

You can use the USB2000+’s USB connectivity with any computer that meets the following
requirements:

• Operating system is one of the following:

• Windows – 98/Me/2000/XP

• Apple Macintosh – OS X version 10.0 or later

• Linux – Red Hat 9 or later, Fedora (any version), Debian 3.1 (Sarge), and SUSE (9.0 or

later)

• Ocean Optics’ SpectraSuite software application installed and configured for use.

EEPROM Utilization

An EEPROM memory chip in each USB2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The SpectraSuite software
application reads these values directly from the spectrometer, enabling the ability to “hot-swap”
spectrometers between computers without entering the spectrometer coefficients manually on each
computer.

About SpectraSuite

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

2 270-00000-000-02-0108

1: Introduction

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation

).

Sampling System Overview

How Sampling Works

Ocean Optics components function in a sampling system as follows:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light from the light source transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.

6. The spectrometer passes the sample information to SpectraSuite.

7. SpectraSuite compares the sample to the reference measurement and displays processed spectral
information.

Interface Options

The USB2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a computer via a USB or serial port. However, you must create custom
software if using the serial port. SpectraSuite software is available if you are connecting via the USB port.

Shipment Components

The following information and documentation ships with the USB2000+ Spectrometer:

 Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.

 USB Cable (USB-CBL-1)

Use this cable to connect your spectrometer to a computer running on a Windows, Mac or Linux
operating system.

270-00000-000-02-0108 3

1: Introduction

 Wavelength Calibration Data Sheet

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. SpectraSuite Operating Software reads this calibration data from
your spectrometer when it interfaces to a computer via the USB port.

Note

Please save the Wavelength Calibration Data Sheet for future reference.

 Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and
spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these
files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources
CD.

Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the Software and Technical
Resources CD package.

Additional Equipment Needed

 SpectraSuite Operating Software

See About SpectraSuite

for more information.

Other Accessories Available

Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.

 Fibers
 Light Sources
 Integrated Sampling Systems
 Cuvettes
 Filter Holders

4 270-00000-000-02-0108

1: Introduction

270-00000-000-02-0108 5

Chapter 2

Installing the USB2000+

Overview

You must install the SpectraSuite software application prior to connecting the USB2000+ Spectrometer to
the computer. The SpectraSuite software installation installs the drivers required for USB2000+
installation. If you do not install SpectraSuite first, the system will not properly recognize the USB2000+.

If you have already connected the USB2000+ to a computer running on a Windows platform prior to
installing SpectraSuite, consult Chapter 3: Troubleshooting
USB2000+ installation.

Note the spectrometer(s) that you have installed are listed in the Data Sources pane.

for information on correcting a corrupt

USB2000+ Installation

This section contains instructions for connecting the USB2000+ via both USB and serial modes.

USB Mode

Note

The USB port on a computer can power up to five USB2000+ spectrometer channels.
Systems with more than five channels require a powered USB hub.

► Procedure

Follow the steps below to connect the USB2000+ to a computer via the USB port:

1. Install SpectraSuite on the destination computer.

2. Locate the USB cable (USB-CBL-1) provided with the USB2000+.

3. Insert the square end of the cable into the side of the USB2000+.

4. Insert the rectangular end of the cable into the USB port of the computer.

If you installed SpectraSuite prior to connecting the USB2000+, the SpectraSuite installs the USB2000+
drivers. If the drivers do not successfully install (or if you connected the USB2000+ to the computer
before installing SpectraSuite), consult Chapter 3: Troubleshooting

.

270-00000-000-02-0108 6

2: Installing the USB2000+

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

Note the spectrometer(s) that you have installed are listed in the Data Sources pane.
Once you install the software and hardware, and establish your sampling system, you are ready to take

measurements.

Serial Port Mode

To use the serial port capacity of the USB2000+ Spectrometer, the computer must be running a 32-bit
version of the Windows operating system.

►

Procedure

Follow the steps below to connect the USB2000+ to the computer via serial port:

1. Connect the serial cable adapter block to the appropriate pins of the USB2000+’s 30-Pin
Accessory Connector.

2. Connect one end of the 9-pin serial cable to the adapter block on the USB2000+, and then
connect the other end to a serial port on the computer.

3. Note the number of the serial port (COM Port) to which you connected the USB2000+ (some
computers may not have numbered ports; handheld computers typically have only one serial
port).

4. Plug the 5 VDC external power supply into an outlet and connect it to the USB2000+.

Connect Spectroscopic Accessories

To find operating instructions for USB2000+-compatible products (such as light sources, sampling
chambers, and probes), consult the Software and Technical Resources CD or the Ocean Optics website at

http://www.oceanoptics.com/technical/operatinginstructions.asp.

USB2000+ Configuration

The USB2000+ can be used with either SpectraSuite or OOIBase32 software when connected to the USB
port. The configuration process differs, depending on whether you are running SpectraSuite or
OOIBase32 software.

Configuring the USB2000+ in SpectraSuite

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

270-00000-000-02-0108 7

2: Installing the USB2000+

Note the spectrometer(s) that you have installed are listed in the Data Sources pane.

Configuring the USB2000+ in OOIBase32

Once you install the USB2000+, you must configure OOIBase32’s Configure Spectrometer options so
that OOIBase32 recognizes the USB2000+ Spectrometer. Consult the OOIBase32 Spectrometer
Operating Software Operating Instructions for detailed instructions on configuring the spectrometer in
OOIBase32 (see Product-Related Documentation

).

8 270-00000-000-02-0108

Chapter 3

Troubleshooting

Overview

The following sections contain information on troubleshooting issues you may encounter when using the
USB2000+ Spectrometer.

USB2000+ Connected to Computer Prior to
SpectraSuite Installation

Windows Operating Systems

If you connected your Ocean Optics USB2000+ device to the computer prior to installing your Ocean
Optics software application on a Windows platform, you may encounter installation issues that you must
correct before your Ocean Optics device will operate properly.

Follow the applicable steps below to remove the incorrectly installed device, device driver, and
installation files.

Note

If these procedures do not correct your device driver problem, you must obtain the

Correcting Device Driver Issues document from the Ocean Optics website:

http://www.oceanoptics.com/technical/engineering/correctingdevicedriverissues.pdf

Remove the Unknown Device from Windows Device Manager

► Procedure

1. Open Windows Device Manager. Consult the Windows operating instructions for your computer
for directions, if needed.

.

2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+"
sign to the immediate left.

270-00000-000-02-0108 9

3: Troubleshooting

Note

Improperly installed USB devices can also appear under the Universal Serial Bus
Controller option. Be sure to check this location if you cannot locate the unknown device.

3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown
Device listing and select the Uninstall or Remove option.

4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown
Device. Click the OK button to confirm the device removal.

5. Disconnect the USB2000+ from your computer.

6. Locate the section in this chapter that is appropriate to your operating system and perform the
steps in the following Remove Improperly Installed Files

section.

Remove Improperly Installed Files

► Procedure

1. Open Windows Explorer.

2. Navigate to the Windows | INF directory.

Note

If the INF directory is not visible, you must disable the Hide System Files and Folders
and Hide File Extensions for Known File Types options in Windows Folder Options.
Access Windows Folder Options from Windows Explorer, under the Tools | Folder
Options menu selection.

3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows
2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory.

4. Navigate to the Windows | System32 | Drivers directory.

5. Delete the EZUSB.SYS file.

6. Reinstall your Ocean Optics application and reboot the system when prompted.

7. Plug in the USB device.

The system is now able to locate and install the correct drivers for the USB device.

Mac Operating Systems

Since there are no device files for the USB2000+ Spectrometer in a Mac operating system, you should not
encounter any problems if you installed the spectrometer before the SpectraSuite software.

10 270-00000-000-02-0108

3: Troubleshooting

Linux Operating Systems

For Linux operating systems, all you need to do is install the SpectraSuite software, then unplug and
replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged users
permission to use newly connected hardware. There isn’t any long-term harm to plugging in the device
before installing the software.

270-00000-000-02-0108 11

3: Troubleshooting

12 270-00000-000-02-0108

Appendix A

Calibrating the Wavelength of

the USB2000+

Overview

This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
USB2000+.

About Wavelength Calibration

You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:

λ

= I + C1 p + C

p

Where:

λ

= the wavelength of pixel p

I = the wavelength of pixel 0

= the first coefficient (nm/pixel)

C

1

= the second coefficient (nm/pixel2)

C

2

= the third coefficient (nm/pixel3)

C

3

You will be calculating the value for I and the three Cs.

2

p

+ C

2

3

p

3

270-00000-000-02-0108 13

A: Calibrating the Wavelength of the USB2000+

Calibrating the Spectrometer

Preparing for Calibration

To recalibrate the wavelength of your spectrometer, you need the following components:

• A light source capable of producing spectral lines

Note

Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an
HG-1, you need a light source that produces several (at least 4-6) spectral lines in the
wavelength region of your spectrometer.

• A USB2000+ spectrometer

• An optical fiber (for spectrometers without a built-in slit, a 50-

• A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-

order linear regressions

µm fiber works best)

Note

If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak
and AnalysisTookPak-VBA.

Calibrating the Wavelength of the Spectrometer

► Procedure

Perform the steps below to calibrate the wavelength of the spectrometer:

1. Place SpectraSuite into Scope mode and take a spectrum of your light source. Adjust the
integration time (or the A/D conversion frequency) until there are several peaks on the screen that
are not off-scale.

2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.

3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.

4. Use the spreadsheet program or calculator to create a table like the one shown in the following
figure. In the first column, place the exact or true wavelength of the spectral lines that you used.

In the second column of this worksheet, place the observed pixel number. In the third column,
calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.

14 270-00000-000-02-0108

A: Calibrating the Wavelength of the USB2000+

Independent

Variable

Dependent

Variables

Values Computed

from the Regression

Output

True Wavelength (nm) Pixel # Pixel # 2 Pixel #

253.65

296.73

302.15

313.16

334.15

365.02

404.66

407.78

435.84

546.07

576.96

579.07

696.54

706.72

727.29

738.40

751.47

175
296
312
342
402
490
604
613

694
1022
1116
1122
1491
1523
1590
1627
1669

30625
87616

97344
116964
161604
240100
364816
375769
481636

1044484
1245456
1258884
2223081
2319529
2528100
2647129
2785561

1067462648
1389928896
1412467848
3314613771
3532642667
4019679000
4306878883
4649101309

3

5359375
25934336
30371328
40001688
64964808

117649000
220348864
230346397
334255384

Predicted

Wavelength

253.56

296.72

302.40

313.02

334.19

365.05

404.67

407.78

435.65

546.13

577.05

579.01

696.70

706.62

727.24

738.53

751.27

Difference

0.09

0.01

-0.25

0.13

-0.05

-0.04

-0.01

0.00

0.19

-0.06

-0.09

0.06

-0.15

0.10

0.06

-0.13

0.19

5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the
spreadsheet program, find the functions to perform linear regressions.

• If using Quattro Pro, look under Tools | Advanced Math

• If using Excel, look under Analysis ToolPak

6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number
squared, and the pixel number cubed as the independent variables (X). After executing the
regression, you will obtain an output similar to the one shown below. Numbers of importance are
noted.

Regression Statistics

Multiple R 0.999999831
R Square 0.999999663 R Squared
Adjusted R Square 0.999999607
Standard Error 0.125540214
Observations 22

Intercept

Coefficients

Standard Error

Intercept 190.473993 0.369047536 First coefficient
X Variable 1 0.36263983 0.001684745
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10 Second coefficient

Third coefficient

270-00000-000-02-0108 15

A: Calibrating the Wavelength of the USB2000+

7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.

Keep these values at hand.

Saving the New Calibration Coefficients: USB
Mode

Ocean Optics programs wavelength calibration coefficients unique to each USB2000+ onto an EEPROM
memory chip in the USB2000+.

You can overwrite old calibration coefficients on the EEPROM if you are using the USB2000+ via the
USB port.

Procedure

►

To save wavelength calibration coefficients using the USB mode, perform the following steps:

1. Ensure that the USB2000+ is connected to the computer and that you have closed all other
applications.

2. Point your browser to http://www.oceanoptics.com/technical/softwaredownloads.asp
scroll down to Microcode. Select USB EEPROM Programmer.

3. Save the setup file to your computer.

4. Run the Setup.exe file to install the software. The Welcome screen appears.

5. Click the Next button. The Destination Location screen appears.

6. Accept the default installation location, or click the Browse button to specify a directory. Then,
click the Next button. The Program Manager Group screen appears.

7. Click the Next button. The Start Installation screen appears.

8. Click the Next button to begin the installation. Once the installation finishes, the Installation
Complete screen appears.

9. Click the Finish button and reboot the computer when prompted.

10. Navigate to the USB EEP ROM Programmer from the Start menu and run the software.

11. Click on the desired USB2000+ device displayed in the left pane of the USB Programmer
screen.

12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the Calibrating the

Wavelength of the Spectrometer

13. Repeat Step 12 for all of the new values.

section in this appendix.

and

14. Click on the Save All Values button to save the information, and then Exit the USB Programmer
software.

The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
USB2000+.

16 270-00000-000-02-0108

Appendix B

External Triggering

Overview

The USB2000+ supports four triggering modes, which are set with the Trigger Mode command. Details
of each triggering mode follow.

Normal (Free Running)

In this mode, the USB2000+ uses the user-defined integration clock and continuously scans the CCD
array. All of the signals described in the previous section are provided by the spectrometer to the CCD
automatically based on the integration time selected in the software by the user.

External Software Trigger

In this mode, the ExtTrigIn signal acts as an enable switch. If ExtTrigIn is kept HIGH, the USB2000+
will acquire spectra as if it was in the normal free running mode. If ExtTrigIn is kept LOW, the
USB2000+ will not acquire spectra.

External Hardware Trigger

On the USB2000+, Ocean Optics has altered the functionality of the External Hardware Trigger function
and reprogrammed the Single Strobe signal function. These alterations of the USB2000 enable you to
operate in the Variable Delay Mode without the presence of an external trigger signal. The USB2000+
will simulate the signal to trigger the appropriate circuitry in the spectrometer.

The table below summarizes the capacity of the USB2000+ in Variable Delay Mode:

Parameter Value in Normal Acquisition Mode Value in Variable Delay Mode

Integration time User controlled 5 ms fixed

Strobe delay

Jitter < 25 ns < 25 ns

0 µs 2 – 511 µs + 3 µs

270-00000-000-02-0108 17

B: External Triggering

Timing Diagram for Variable Delay Mode

External Synchronization Trigger

In this mode of the USB2000+, the integration clock is essentially input into the spectrometer. The
synchronized trigger must have a frequency range of 5ms to 60s (shutter mode cannot be used in External
Synchronization Trigger mode). If no External Trigger Input Signal is applied, the spectrometer will not
take any spectra, and the software will appear “frozen” until an External Trigger Input Signal is detected.
With a continuous ExtTrigIn, the spectrometer will provide the required signals to the CCD as shown
below:

The time between when ExtTrigIn goes HIGH and the beginning of the integration period is specified to
be between 8µs<t

<10µs. The ExtTrigIn signal does not need to have an even duty cycle, but the time

SU_ST

between rising edges should remain constant for a given integration time.

18 270-00000-000-02-0108

External Synchronization Trigger

Appendix C

Specifications

Overview

This appendix contains information on spectrometer operation, specifications, and system compatibility.
It also includes accessory connector pinout diagrams and pin-specific information.

How the USB2000+ Works

Below is a diagram of how light moves through the optical bench of an USB2000+ Spectrometer. The
optical bench has no moving parts that can wear or break; all the components are fixed in place at the time
of manufacture. Items with an asterisk (*) are user-specified.

USB2000+ Spectrometer with Components

See USB2000+ Components Table
numbered component in the USB2000+ Spectrometer in this diagram.

270-00000-000-02-0108 19

on the following page for an explanation of the function of each

C: Specifications

USB2000+ Components Table

Ocean Optics permanently secures all components in the USB2000+ at the time of manufacture. Only
Ocean Optics technicians can replace interchangeable components, where noted.

Item Name Description

1

2 Slit

3 Filter

4

5 Grating

6

7

SMA 905
Connector

Collimating
Mirror

Focusing
Mirror

L2 Detector
Collection
Lens

Secures the input fiber to the spectrometer. Light from the input fiber enters the
optical bench through this connector.

A dark piece of material containing a rectangular aperture, which is mounted directly
behind the SMA Connector. The size of the aperture (200 µm) regulates the amount
of light that enters the optical bench and controls spectral resolution.

Restricts optical radiation to pre-determined wavelength regions. Light passes
through the Filter before entering the optical bench.

A SAG+, Ag-coated mirror focuses light entering the optical bench towards the
Grating of the spectrometer.

Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,
and then reflects off the Collimating Mirror onto the Grating.

A #3 (600 lines per millimeter, blazed at 500 nm) grating diffracts light from the
Collimating Mirror and directs the diffracted light onto the Focusing Mirror.

A SAG+, Ag-coated mirror receives light reflected from the Grating and focuses
first-order spectra onto the detector plane.

Attaches to the Detector to increase light-collection efficiency. It focuses light from a
tall slit onto the shorter Detector elements.

The L2 Detector Collection Lens should be used with large diameter slits or in
applications with low light levels. It also improves efficiency by reducing the effects
of stray light.

Collects the light received from the Focusing Mirror or L2 Detector Collection Lens

8

9 LVF Filters

20 270-00000-000-02-0108

Detector

and converts the optical signal to a digital signal. Each pixel on the Detector
responds to the wavelength of light that strikes it, creating a digital response. The
spectrometer then transmits the digital signal to the SpectraSuite application.

Optional Linear Variable Filters (LVF) construct systems with excellent separation of
excitation and fluorescence energy. LVF-L Linear low-pass filters fine tune the
excitation source for maximum signal with minimum overlap. LVF-H high-pass filters
are available for the detection side. These filters are optional.

C: Specifications

USB2000+ Specifications

The following table provides specification information for the USB2000+.

Specification Value

Spectrometer USB2000+

Grating Options 14 different gratings, UV through Shortwave NIR

Detector 2048-element linear silicon CCD array; L2 Collection Lens

Wavelength range 200-1100 nm

Entrance aperture 5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit)

AgPlus Mirrors Provide reflectivity >95% over the visible and NIR wavelength range and

over a wide range of angles of incidence

Integration time 1 ms to 65 seconds

Data transfer rate Full scans (2048 wavelengths) into memory every 13 milliseconds

Time acquisition approximately every 25 milliseconds

Optical resolution ~0.3-10.0 nm FWHM

Stray light ~0.05% at 600 nm, <0.10% at 435 nm

Power consumption 250 mA @ 5 VDC

Dimensions 89.1 mm x 63.3 mm x 34.4 mm

Weight 190 g

Computer interface Universal Serial Bus (RS-232 available on side connector)

Computer requirements* IBM-compatible PC with Pentium or better microprocessor

32 MB RAM (16 MB)

Windows 98/Me/2000/XP, Mac OS X and Linux when using the USB
interface on desktop or notebook PCs.

Any 32-bit Windows operating system when using the serial port on
desktop or notebook PCs. Windows

CE 2.11 and above when using the serial port on handheld PCs.

Operating software SpectraSuite (for an additional $199)

Fiber optic connector SMA 905 to single-strand optical fiber

* Download free software to analyze your computer's USB readiness by clicking here.

270-00000-000-02-0108 21

C: Specifications

20-Pin Accessory Connector Pinout

The USB2000+ features a 20-pin Accessory Connector, located on the front of the unit as shown:

Location of USB2000+ 20-Pin Accessory Connector

20-Pin Accessory Connector Pinout Diagram

When facing the 20-pin Accessory Connector on the front of the vertical wall of the USB2000+, pin
numbering is as follows:

18 16 14 12 10 8 6 4 2 A2
17 15 13 11 9 7 5 3 1 A1

20-Pin Accessory Connector Pinout Diagram

20-Pin Accessory Connector – Pin Definitions and
Descriptions

The following table contains information regarding the function of each pin in the USB2000+’s 10-Pin
Accessory Connector:

Pin
#

A1 SPI_CLK Output

A2 SPICS_OUT Output

Function Input/Output Description

The SPI Clock signal for communications to other SPI
peripherals

The SPI Chip/Device Select signal for communications to other
SPI peripherals

V

, V

1

22 270-00000-000-02-0108

CC

5V

, or

USB

IN

Input or
Output

Input power pin for USB2000+ – When operating via USB, this
pin can power other peripherals – Ensure that peripherals
comply with USB specifications

C: Specifications

Pin
#

2 RS232 Tx Output

3 RS232 Rx Input

4 Lamp Enable Output

5

Function Input/Output Description

RS232 transmit signal – Communicates with a computer over
DB9 Pin 2

RS232 receive signal – Communicates with a computer over
DB9 Pin 3

TTL signal driven Active HIGH when the Lamp Enable
command is sent to the spectrometer

Continuous
Strobe

Output

TTL output signal used to pulse a strobe – Divided down from
the master clock signal

6 Ground Input/Output Ground

7

External
Trigger In

Input

TTL input trigger signal – See External Triggering Options
document for info

TTL output pulse used as a strobe signal – Has a programmable

8 Single Strobe Output

delay relative to the beginning of the spectrometer integration
period

2

The I

9 I2C SCL Input/Output

C clock signal for communications to other I2C

peripherals.

10 I2C SDA Input/Output The I2C Data signal for communications to other I2C peripherals.

11 MOSI Output

12 MISO Input

13 GPIO-1 Input/Output

14 GPIO-0 Input/Output

15 GPIO-3 Input/Output

16 GPIO-2 Input/Output

17 GPIO-5 Input/Output

18 GPIO-4 Input/Output

19 GPIO-7 Input/Output

20 GPIO-6 Input/Output

The SPI Master Out Slave In (MOSI) signal for communications to
other SPI peripherals

The SPI Master In Slave Out (MISO) signal for communications to
other SPI peripherals

Master clock

Base clock

Integration clock

Reserved

Acquire spectra (Read Enable)

Reserved

SH CCD pin

ICG CCD pin

270-00000-000-02-0108 23

C: Specifications

24 270-00000-000-02-0108

Index

20

20-pin accessory connector

diagram, 22
pin definitions, 22

A

accessories, 4, 7
accessory connector

pinout, 22
additional equipment, 4
Adobe Acrobat Reader, 4

C

Calibrating, iii, 13
calibration, 13

preparing for, 14

procedure, 14
calibration coefficients

saving in USB mode, 16
CCD Detector, 20
collimating mirror, 20
Components Table, 20
configuration, 7
configuring, 8

D

F

features, 2
filter, 20
focusing mirror, 20

G

grating, 20

I

Installation, 6

Serial Port mode, 7

USB mode, 6
installed filter, 20
Interface, 3

L

L2 Detector Collection Lens, 20
Lens, 20

M

memory chip, 2
mirror, 20

Detector Collection Lens, 20
document

audience, iii
purpose, iii
summary, iii

E

EEPROM, 2
external triggering, 17

270-00000-000-02-0108 25

O

OOIBase32, 8
Options

Interface, 3

P

packing list, 3
passwords, 4
product-related documentation, iii

Index

S

Sampling

System, 3
Serial Port mode, 7
service, iv
setup, 6
shipment components, 3
slit, 20
SMA Connector, 20
Software and Resources Library CD, 4
specifications, 19, 21
SpectraSuite, 2
spectroscopic accessories, 7
System Requirements, 2

T

Timing Diagram, 18
triggering, 17
troubleshooting

Linux systems, 11

Mac systems, 10

Windows systems, 9
Troubleshooting, 9

U

upgrades, iv
USB mode, 6

V

Variable Delay Mode, 17

W

Wavelength Calibration

about, 13
Wavelength Calibration Data File, 4
Wavelength Calibration Data Sheet, 4

26 270-00000-000-02-0108