Ocean Optics MonoScan2000 User Manual

Scanning Monochromator

(MonoScan2000)

Installation and Operation Manual

Document Number 000-10000-150-02-201011

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

Ocean Optics Mikropack

Maybachstraße 11, D73760, Ostfildern, Germany

Phone [phone]
Fax [phone]

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Mikropack sales inquiries)
[e-mail] (Questions about orders)
[e-mail] (Technical support)

Copyright © 2009 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical,
photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise
circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

Microsoft, Windows, Windows 95, Windows 98, Windows Me, Windows NT, Windows 2000, Windows XP and Excel are either registered trademarks or
trademarks of Microsoft Corporation.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on
an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the
information contained in this manual

Important Safety Notices

1. Read all safety notices and operating instructions before operating this unit.

2. Inspect the item for transport damage before using the 24VDC power supply for the first time.

3. Adhere to all warning stickers on the unit and all warnings contained in this manual.

Warranty

Mikropack GmbH warrants to the original user of this instrument that it shall be free of any defects
resulting from faulty manufacture of this instrument for a period of 12 months from the original data of
shipment.

This instrument should not be used for any Clinical or Diagnostic purposes. Data generated in these
areas is not warranted in any way by Mikropack GmbH. Any defects covered by this Warranty shall be
corrected either by repair or by replacement, as determined by Mikropack GmbH.

There are no warranties that extend beyond the description herein.

This Warranty is in lieu of, and excludes, any and all other warranties or representations expressed,
implied, or statutory, including merchantability and fitness, as well as any and all other obligations or
liabilities of Mikropack GmbH including, but not limited to, special or consequential damages. No
person, firm, or corporation is authorized to assume for Mikropack GmbH. Any additional obligation or
liability not expressed provided for herein except in writing duly executed by an officer of Mikropack
GmbH:

MIKROPACK GmbH

Maybachstraße 11
D-73760 Ostfildern

Tel.: [phone] • Fax.: [phone]

[e-mail]

e-mail:

Warranty Handling

► Procedure

Follow the procedure below to process a warranty claim:

1. Determine the problem or fault with your local distributor.

2. If a problem is evident, obtain an RMA number from your local distributor.

000-10000-150-02-201011 A

Important Safety Notices

3. Send the equipment to the local distributor for repair. If the item is under warranty, shipping will
be free-of-charge both ways.

4. Contact your distributor for repair and delivery time. If the item is out of warranty, your
distributor will provide a repair cost to you. In this situation, the distributor will not proceed with
the repair until you order it.

Your system will be shipped back to you free of charge with insurance (if under warranty).

B 000-10000-150-02-201011

Table of Contents

About This Manual .............................................................................................................iii

Document Purpose and Intended Audience..............................................................................iii

Document Summary..................................................................................................................iii

What’s New in this Document .................................................................................................. iii

Product-Related Documentation ............................................................................................... iii

Upgrades ............................................................................................................................iv

Chapter 1: Setup...................................................................................1

Overview.............................................................................................................................1

Applications ........................................................................................................................1

Set-up .................................................................................................................................1

Unpacking the MonoScan2000..........................................................................................2

Contents .............................................................................................................................2

Quick Start..........................................................................................................................2

Chapter 2: Programming Information ................................................5

MonoScan-2000 Interface-DLL..........................................................................................5

Direct Serial Communication (ASCII Commands).............................................................7

ASCII Commands......................................................................................................................7

Stored Data ............................................................................................................................... 8

End User License Agreement for Software (EULA) ..........................................................9

Appendix A: Specifications.................................................................11

Operating Environment ......................................................................................................11

Physical Specifications.......................................................................................................11

Index ......................................................................................................13

000-10000-150-02-201011 i

Table of Contents

ii 000-10000-150-02-201011

About This Manual

Document Purpose and Intended Audience

This document provides you with an installation section to get your system up and running.

What’s New in this Document

This version of the Scanning Monochromater (MonoScan2000) Installation and Operation Manual
updates the photos and the interface information.

Document Summary

Chapter Description

Chapter 1: Setup

Chapter 2: Programming Information

Appendix A: Specifications

Contains a list of package contents and unpacking
instructions.

Provides programming information for the
MonoScan-2000 software, as well as the EULA.

Contains operating environment specifications, as
well as other physical details of the product.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the
of the web page.

You can also access operating instructions for Ocean Optics products on the

Resources

Engineering-level documentation is located on our website at

CD included with the system.

Search by Model Number field at the bottom

Software and Technical

Technical → Engineering Docs.

000-10000-150-02-201011 iii

About This Manual

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact an Ocean Optics Application Scientist for specific
instructions when returning a product.

iv 000-10000-150-02-201011

Chapter 1

Setup

Overview

The MonoScan-2000 is a small-bandwidth fiber optic scanning monochromator. This monochromator is
specially made to achieve a high optical throughput in fluorescence and luminescence applications,
resulting in an intensive spectral signal.

The unique system design makes it easily compatible with all Ocean Optics and Mikropack light sources,
accessories and spectrometers. Its RS232 interface and 12VDC power make it convenient to use, and
even possible for mobile applications.

Applications

 Variable monochromatic lightsource: If the MonoScan-2000 is combined to our fiber optic

lightsources (e.g., HL-2000-HP, HPX-2000; DH-2000; DH-2000-BAL)

 Single variable Wavelength detection: If combined with a single Si-Detector

 Programmable Fluorescence Scanner: Two MonoScan-2000s combined can be used as a fully

programmable fluorescence scanner if the unit is combined with a high-power lightsource (e.g.,
HPX-2000), going to the sample via fiber, detecting via fiber, then going through the second
MonoScan-2000 combined with a single Si-Detector.

Set-up

The following sections provide instructions on unpacking and setting up your MonoScan-2000.

000-10000-150-02-201011 1

1: Setup

Unpacking the MonoScan-2000

► Procedure

1. Unpack the MonoScan-2000 carefully. Dropping this instrument can cause permanent damage.

2. Inspect the outside of the instrument and make sure that there is no damage. Do not use the
instrument if damage is present. Contact your dealer for repair or replacement information, if
necessary.

3. Use this instrument in a clean laboratory environment.

4. Submit the Registration Card to for warranty and support purposes.

Contents

Your MonoScan-2000 package should contain the following:

 MonoScan-2000 main system

 MonoScan-2000 12VDC power supply

Caution

Before using the power supply of the MonoScan-2000 for the first time, inspect the item
for transport damage. Be sure to adhere to all warnings on the unit and in this operational
manual.

 Software CD (contains MonoScan-2000 software and calibration software)

 Ocean Optics Software & Technical Resources CD (contains manuals)

 RS232 connection cable

Quick Start

► Procedure

1. Install the software delivered with the MonoScan-2000 (double-click on Setup.exe)

2. Connect the power supply to main connection.

3. Connect the power supply to the MonoScan-2000.

2 000-10000-150-02-201011

4. Connect your PC to the MonoScan-2000

5. Power-on the MonoScan-2000.

1: Setup

Front Panel

Rear Panel

6. Start the MonoScan-2000 software.

000-10000-150-02-201011 3

1: Setup

7. Go to the Connection settings menu and select the Comport to which the MonoScan-2000 is
connected.

8. Go to Connection settings menu and select Connect to establish a connection between the PC and
the MonoScan-2000 (the MonoScan-2000 will run to its internal reference and will be set to
250nm).

9. If the device is found on the chosen Comport, the following message appears:

All buttons then become enabled.

10. Set another wavelength by clicking on one of the buttons (<10nm, <1nm, 1nm >, 10nm >) or
enter a wavelength and click

Goto.

11. When the measurements are finished, close the connection between the PC and the MonoScan­2000 in the

Connection settings – Disconnect menu.

4 000-10000-150-02-201011

Programming Information

MonoScan-2000 Interface-DLL

The following functions are exported by MonoScandrv.dll:

MONO_OpenConnection

long __stdcall MONO_OpenConnection(char* PortName);

Returns the handle value for the specified Comport as long integer.
This value has to be stored locally for all other functions.
Returns a negative value in case of error.
Portname has to be e.g. “Com1”, “Com2”

Chapter 2

MONO_CloseConnection

short __stdcall MONO_CloseConnection(long Handle);

Close the connection opened before.
Returns 1 on success else -1
long Handle: Handle of the comport returned by MONO_OpenConnection

MONO_GetDeviceData

short __stdcall MONO_GetDeviceData(long Handle, unsigned short Node, long* CalibrationData, char*
SerialNo);

Get the calibration data and the serialnumber of the device
long Handle: The handle to the comport returned by MONO_OpenConnection
unsigned short Node: has to be 0 (zero)
long* CalibrationData: Array of long integers, has to contain 92 elements
char* SerialNo: The serialnumber of the Device, at least 10 characters

000-10000-150-02-201011 5

2: Programming Information

MONO_SetWavelength

short __stdcall MONO_SetWavelength(long Handle, unsigned short Node, short WavelengthInNm, long*
CalibrationData);

Set the MonoScan-2000 to a specified wavelength in nm.
Returns 1 on success else a negative number.
Long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node: must be 0 (zero)
short WavelengthInNm: desired wavelength in nm
long* CalibrationData: Array filled by MONO_GetDeviceData

MONO_GetWavelength

short __stdcall MONO_GetWavelength(long Handle, unsigned short Node, long* CalibrationData);

returns the actual position of the MonoScan-2000 in nm.
Long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node:must be 0 (zero)
long* CalibrationData: Array filled by MONO_GetDeviceData

MONO_RunReferenceSequence

short __stdcall MONO_RunReferenceSequence(long Handle,unsigned short Node);

MonoScan will run to its internal reference point and reset the internal position
Long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node:must be 0 (zero)

MONO_HomingRunActive

short __stdcall MONO_HomingRunActive(long Handle,unsigned Node);

call this function sequentially to see if reference sequence has completed
returns 1 if reference sequence is still active else 0.
Long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node:must be 0 (zero)

MONO_PositionReached

short __stdcall MONO_PositionReached(long Handle,unsigned short Node);

call this function to see if the desired position has been reached.
Returns 0 if still moving else 1
Long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node:must be 0 (zero)

6 000-10000-150-02-201011

2: Programming Information

MONO_SetPosition

short __stdcall MONO_SetPosition(long Handle,unsigned short Node,long Position);

call this function to set an absolute user defined position. Wavelength is not linear to position
Returns 0 in case of error else 1
long Handle: Handle of the comport returned by MONO_OpenConnection
unsigned short Node: must be 0 (zero)
long position: absolute position value

MONO_GetPosition

long __stdcall MONO_GetPosition(long Handle,unsigned short Node);

call this function to get the absolute position in steps (arbitrary unit)
long Handle: handle of the comport returned by MONO_OpenConnection
unsigned short Node: must be 0 (zero)

Direct Serial Communication (ASCII Commands)

If you do not want to use the DLL-functions you can directly communicate with the MonoScan2000
using ASCII commands.

► Procedure

The following steps are required:

1. Establish serial connection from PC to MonoScan-2000

2. Read device information such as calibration data and serial number (not necessarily needed)

3. Set an absolute position. Repeat this step as needed.

4. Close serial connection

ASCII Commands

Command Description Example

GOHOSEQ Reference run is executed. Runs to the lower endpoint

(approx 200nm) and sets this position to 0 (zero)

LA<Pos> Sets absolute position in steps

Command M starts positioning

LR<Pos> Sets relative position in steps

Command M starts positioning

GOHOSEQ [CR]

LA-5000 [CR]M [CR]

LR100 [CR]M [CR]

POS Returns actual position in steps POS [CR]

000-10000-150-02-201011 7

2: Programming Information

Command Description Example

HO Sets current position to 0 (zero). A reference run executed

before will be lost

GPROGSEQ Returns the data stored in the MonoScan-2000 GPROGSEQ [CR]

HO [CR]

Note

All Commands have to be completed by Carriage return [CR]

Stored Data

Stored Data Comment

JMP1 Internal

11220001 Serial Number

0 Absolute position for 200nm (not valid)

0

-497

-1087

-1665

-2201 Absolute position for 250nm

-2750

-3306

... Some data left out

-46911 Absolute Position for 1100nm (not valid)

A1 Internal

LR5000 Internal

M Internal

delay20 Internal

gohoseq Start reference run

Every time the MonoScan-2000 is powered-on, a reference run is performed.

8 000-10000-150-02-201011

2: Programming Information

End User License Agreement for Software (EULA)

This End-User License Agreement ("EULA") is a legal agreement between you (either an

individual or a single entity) and Mikropack GmbH ("Mikropack") for the Mikropack MonoScandrv
software, which includes computer software and may include associated media, printed materials, and
"online" or electronic documentation ("SOFTWARE"). By installing, copying, or otherwise using the
SOFTWARE, you agree to be bound by the terms of this EULA. If you do not agree to the terms of this
EULA, do not install, copy or use the SOFTWARE.

The SOFTWARE is protected by copyright laws and international copyright treaties, as well as other
intellectual property laws and treaties. The SOFTWARE is licensed, not sold.

1. License to Use

Mikropack grants you a non-exclusive and non-transferable license for the internal use only of the
SOFTWARE and any error corrections provided by Mikropack.

2. Restrictions

SOFTWARE is confidential and copyrighted. Title to SOFTWARE and all associated intellectual
property rights is retained by Mikropack and/or its licensors. You may not make copies of SOFTWARE,
other than a single copy of SOFTWARE for archival purposes. Unless enforcement is prohibited by
applicable law, you may not modify, decompile, or reverse engineer SOFTWARE.

No right, title or interest in or to any trademark, service mark, logo or trade name of Mikropack or its
licensors is granted under this EULA.

3. Limited Warranty

Mikropack warrants to you that for a period of six (6) months from the date of shipping, as evidenced by
a copy of the receipt, the media on which SOFTWARE is furnished (if any) will be free of defects in
materials and workmanship under normal use. Except for the foregoing, SOFTWARE is provided "AS
IS". Your exclusive remedy and Mikropack's entire liability under this limited warranty will be at
Mikropack's option to replace SOFTWARE media or refund the fee paid for SOFTWARE

4. Disclaimer of Warranty

Unless specified in this EULA, all express or implied conditions, representations and warranties,
including any implied warranty of merchantability, fitness for a particular purpose or noninfringement are
disclaimed, except to the extent that these disclaimer are held to be legally invalid.

5. Limitation of Liability

To the extent not prohibited by law, in no event will Mikropack or its licensors be liable for any lost
revenue, profit or data, or for special, indirect, consequential, incidental or punitive damages, however
caused regardless of the theory of liability, arising out of or related to the use of or inability to use
SOFTWARE, even if Mikropack has been advised of the possibility of such damages. In no event will
Mikropack's liability to you, whether in contract, tort (including negligence), or otherwise, exceed the
amount paid by you for Software under this EULA. The foregoing limitations will apply even if the above
stated warranty fails of its essential purpose.

000-10000-150-02-201011 9

2: Programming Information

10 000-10000-150-02-201011

Appendix A

Specifications

This section provides information on the operating environment and physical specifications of the
MonoScan-2000.

Operating Environment

The following table provides information on optimizing the operating environment of your MonoScan-

2000.

Operating Environment The MonoScan-2000 Unit . . .

Moisture Is designed for operation in dry rooms only.

Ventilation Should be situated so that its location or position does not interfere

with proper ventilation.

Heat Should be situated away from any device that emits excessive heat.

Object and Liquid Entry Should be positioned so that objects do not fall on top of the unit.

Additionally, ensure that no liquids are spilled into the enclosure
through openings.

Power Sources The unit should be connected to a power supply only of the type

described in the operating instructions or as marked on the unit.

Physical Specifications

Specification Value

Wavelength range 300 – 700 nm

FWHM 4 – 9 nm (400 –1000 fiber)

Holographic grating 1250 l/mm, Blaze 350nm

Accuracy <0.5nm

Repeatability 0.2nm

Transition speed (wavelength-to-wavelength) Approximately 3s 300-700nm 1nm step ~ 15ms

Dispersion Approximately 10nm/mm

000-10000-150-02-201011 11

A: Specifications

Specification Value

Optical throughput (1000µm fiber) >50% @ 350nm, >30%@500nm

Interface RS-232

Connectors SMA 905

Power Requirements 12 VDC max. 1,2A (WT-24V-E), 400 mA

Dimensions (LxWxH) 148 x 112 x 132 mm

Weight 1.2 kg

Temperature range

5°C – 35°C

Transmission Graph

12 000-10000-150-02-201011

applications, 1
ASCII commands, 7

A

Index

package contents, 2
product-related documentation, ii
programming information, 5

S

D

document

audience, ii
purpose, ii
summary, ii

E

EULA, 9

O

operating environment, 11

P

setup, 1
set-up, 1
specifications, 11

U

unpacking procedure, 1
upgrades, ii

W

warranty, A
what's new, iii

000-10000-150-02-201011 13

Index

14 000-10000-150-02-201011