Ocean Optics HR4000CG-UV-NIR User Manual

HR4000 and HR4000CG-UV-NIR Series

High-Resolution Fiber Optic Spectrometers

HR4000 / HR4000CG-UV-NIR

Installation and Operation Manual

Document Number 210-00000-000-02-0908

Offices:	Ocean Optics, Inc. World Headquarters
	830 Douglas Ave., Dunedin, FL, USA 34698
	Phone	727.733.2447
	Fax	727.733.3962
	8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST
E-mail:	[e-mail]		(General sales inquiries)
	[e-mail]		(Questions about orders)
	[e-mail]		(Technical support)

Additional	Ocean Optics Asia
Offices:	666 Gubei Road, Kirin Tower, Suite 601B, Changning District,
	Shanghai, PRC. 200336
	Phone	86.21.5206.8686
	Fax	86.21.5206.8686
	E-Mail	[e-mail]

Ocean Optics Europe

Sales and Support Center

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]

E-Mail [e-mail]

Regional Headquarters

Maybachstrasse 11

73760 Ostfildern

Phone [phone]

Fax [phone]

E-Mail [e-mail]

Copyright © 2001-2008 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.

This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual.

Table of Contents

About This Manual ..........................................................................................................	iv
Document Purpose and Intended Audience..............................................................................	iv
What’s New in this Document ...................................................................................................	iv
Document Summary..................................................................................................................	iv
Product-Related Documentation ...............................................................................................	iv
Upgrades.........................................................................................................................	v
Service ............................................................................................................................	v
Chapter 1: Introduction ......................................................................	1
Product Overview ............................................................................................................	1
System Requirements .....................................................................................................	2
EEPROM Utilization ..................................................................................................................	2
About Ocean Optics Software ...................................................................................................	2
Sampling System Overview.......................................................................................................	2
How Sampling Works............................................................................................................	2
Modular Sampling Accessories.............................................................................................	3
Interface Options .............................................................................................................	3
Breakout Box .............................................................................................................................	3
Shipment Components....................................................................................................	4
Other Accessories Available ...........................................................................................	5
Chapter 2: Installing the HR4000 .......................................................	7
Overview .........................................................................................................................	7
HR4000 Installation .........................................................................................................	7
USB Mode .................................................................................................................................	7
Serial Port Mode........................................................................................................................	8
Configuring the HR4000..................................................................................................	8
Configuring the HR4000 in SpectraSuite ..................................................................................	8
Configuring the HR4000 in OOIBase32 ....................................................................................	8
Connect Spectroscopic Accessories ...............................................................................	8
External Triggering Options.............................................................................................	9

210-00000-000-02-0908

i

Table of Contents
Chapter 3: Troubleshooting ...............................................................	10
Overview .........................................................................................................................	10
HR4000 Connected to Computer Prior to Operating Software Installation .....................	10
Windows Operating Systems ....................................................................................................	10
Remove the Unknown Device from Windows Device Manager ...........................................	10
Remove Improperly Installed Files........................................................................................	11
Mac Operating Systems ............................................................................................................	11
Linux Operating Systems ..........................................................................................................	12
Older Version of OOIBase32 Installed ............................................................................	12
Appendix A: Calibrating the Wavelength of the HR4000.................	13
Overview .........................................................................................................................	13
About Wavelength Calibration.........................................................................................	13
Calibrating the Spectrometer...........................................................................................	14
Preparing for Calibration............................................................................................................	14
Calibrating the Wavelength of the Spectrometer ......................................................................	14
Saving the New Calibration Coefficients: USB Mode ......................................................	16
Saving the New Calibration Coefficients: Serial Mode ....................................................	17
Appendix B: Specifications................................................................	19
Overview .........................................................................................................................	19
How the HR4000 Works..................................................................................................	19
HR4000 Components Table......................................................................................................	20
HR4000 Specifications ....................................................................................................	21
CCD Detector Specifications.....................................................................................................	21
HR4000 Spectrometer...............................................................................................................	21
System Compatibility.......................................................................................................	22
Compatibility for Desktop or Notebook PCs..............................................................................	22
Compatibility for Handheld PCs ................................................................................................	23
30-Pin Accessory Connector Pinout................................................................................	23
30-Pin Accessory Connector Pinout Diagram...........................................................................	23
30-Pin Accessory Connector – Pin Definitions and Descriptions..............................................	24
30-Pin J2 Accessory Connector - Part Numbers ......................................................................	25
HR4000 15-Pin Accessory Cable Pinout.........................................................................	26

ii	210-00000-000-02-0908

	Table of Contents
Appendix C: HR4000CG-UV-NIR Spectrometer	................................27
HR4000CG-UV-NIR Features .........................................................................................	27
New HC-1 Landis Composite Grating .......................................................................................	27
Variable Order-Sorting Filter......................................................................................................	27
HR4000CG-UV-NIR Spectrometer Specifications...........................................................	27
Index.....................................................................................................	29

210-00000-000-02-0908

iii

About This Manual

Document Purpose and Intended Audience

This document provides the user of HR4000 Series Spectrometers (both HR4000 and HR4000CG-UV-NIR) with instructions for setting up, calibrating and performing experiments with their spectrometer.

What’s New in this Document

This version of the HR4000 and HR4000CG-UV-NIR Series High-Resolution Fiber Optic Spectrometers HR4000 / HR4000CG-UV-NIR Installation and Operation Manual updates the integration time specification and adds service information.

Document Summary

Chapter	Description
Chapter 1: Introduction	Contains descriptive information about the HR4000
	Spectrometer and how sampling works. It also provides a list
	of system requirements, interface options, and shipment
	components.
Chapter 2: Installing the HR4000	Provides installation and configuration instructions.
Chapter 3: Troubleshooting	Contains recommended steps to isolate and correct common
	problems.
Appendix A: Calibrating the	Provides instructions for calibrating the HR4000 Series
Wavelength of the HR4000	Spectrometer.
Appendix B: Specifications	Contains technical specifications and connector pinouts for the
	HR4000 Series Spectrometer.
Appendix C: HR4000CG-UV-NIR	Contains features and specifications unique to the
Spectrometer	HR4000CG-UV-NIR Spectrometer.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate document from the available drop-down lists. Or, use the Search by Model Number field at the bottom of the web page.

210-00000-000-02-0908

iv

About This Manual

•Detailed instructions for SpectraSuite Spectrometer Operating Software are located at: http://www.oceanoptics.com/technical/SpectraSuite.pdf.

•Detailed instructions for OOIBase32 Operating Software are located at:

•http://www.oceanoptics.com/technical/ooibase32.pdf.

•Detailed instructions for the Breakout Box are located at: http://www.oceanoptics.com/technical/HR4_breakout.pdf.

•Detailed instructions for external triggering are located at: http://www.oceanoptics.com/technical/External-Triggering.pdf.

Engineering-level documentation is located on our website at Technical → Engineering Docs.

You can also access operating instructions for Ocean Optics products from the Software and Technical Resources CD that ships with the product.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system. To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a product.

Service

Our Total Technical Service protection plans protect your spectrometer for one year for any contingency - - no matter what the circumstances, including total loss. Under these plans, Ocean Optics will repair or replace your instrument with no deductible or any other out-of-pocket expense required from you. HR service plans include the following:

•TTS-HR: Protects your Ocean Optics HR200/2000+/4000 spectrometer for any contingency for 1 year. Provides for full spectrometer replacement, if necessary. Limited to 1 full replacement per year.

•TTS-HR-ES: This special plan for Ocean Optics HR2000/4000 spectrometers not previously covered protects for any contingency for 1 year. Provides for full spectrometer replacement, if necessary. Limited to 1 full replacement per year. This coverage starts 30 days after purchasing the plan.

In addition, we also have the WAVECAL-USB spectrometer maintenance and calibration service available to recalibrate, clean and perform any adjustments necessary on your spectrometer.

Contact us to learn more about these great service plans.

210-00000-000-02-0908

v

About This Manual

vi	210-00000-000-02-0908

Chapter 1

Introduction

Product Overview

The HR4000 High-Resolution Miniature Fiber Optic Spectrometer provides optical resolution as good as 0.025 nm (FWHM). The HR4000 is responsive from 200-1100 nm, but the specific range and resolution depends on your grating and entrance slit selections.

The HR4000 is perfect for applications where high resolution is necessary, such as absorbance of gases or atomic emission lines (for solution chemistry or for color measurements, the USB4000 is more appropriate).

Data programmed into a memory chip on each HR4000 includes wavelength calibration coefficients, linearity coefficients, and the serial number unique to each spectrometer. Our spectrometer operating software simply reads these values from the spectrometer — a feature that enables hot swapping of spectrometers among computers.

The HR4000 Spectrometer connects to a notebook or desktop computer via USB port or serial port. When connected to the USB port of a computer, the HR4000 draws power from the host computer, eliminating the need for an external power supply.

Ocean Optics HR4000 High-Resolution Fiber Optic Spectrometer

210-00000-000-02-0908

1

1: Introduction

System Requirements

You can use the HR4000’s USB connectivity with any computer that meets the requirements for the spectrometer operating software being used (Windows 98/Me/2000/XP, Mac OS X and Linux). See

About Ocean Optics Software.

Alternately, the HR4000 has a serial port for connecting to PCs, PLCs, and other devices with a Windows 32-bit operating system that support the RS-232 communication protocol. However, this connection method requires an external power supply to power the HR4000, the Breakout Box, and a serial cable.

EEPROM Utilization

An EEPROM memory chip in each HR4000 contains wavelength calibration coefficients, linearity coefficients, and a serial number unique to each individual spectrometer. The OOIBase32 software application reads these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs without entering the spectrometer coefficients manually on each PC.

About Ocean Optics Software

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device, as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform spectroscopic measurements (such as absorbance, reflectance, and emission), control all system parameters, collect and display data in real time, and perform reference monitoring and time acquisition experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation).

OOIBase32 software has been discontinued, but still functions with the HR4000 Spectrometer. Consult the OOIBase32 manual for hardware requirements when using OOIBase32 (see Product-Related Documentation).

Sampling System Overview

How Sampling Works

Ocean Optics components function in a sampling system as follows:

1.The user stores reference and dark measurements to correct for instrument response variables.

2.The light from the light source transmits through an optical fiber to the sample.

3.The light interacts with the sample.

4.Another optical fiber collects and transmits the result of the interaction to the spectrometer.

5.The spectrometer measures the amount of light and transforms the data collected by the spectrometer into digital information.

2	210-00000-000-02-0908

1: Introduction

6.The spectrometer passes the sample information to OOI software.

7.OOI software compares the sample to the reference measurement and displays processed spectral information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spectroscopic accessories for use with the HR4000. Most of our spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the sampling system components is as easy as unscrewing a connector and replacing an accessory.

Interface Options

The HR4000 has both USB and serial port connectors (with the use of an adapter), enabling you to connect the spectrometer to a desktop or notebook computer via a USB port or to a desktop, notebook, or to handheld PC via a serial port. However, you must create custom software if using the serial port. SpectraSuite software is available if you are connecting via the USB port.

	Computer	Operating System Requirements	Part Needed
	Interface
		SpectraSuite: Windows2000/XPfor PC,
		OS X version 10.0 or later for Mac, or
	Computer via	Red Hat 9 or later, Fedora (any	USB-CBL-1
	USB Port	version), Debian 3.1 (Sarge), and SUSE	(included)
		(9.0 or later) for Linux
		OOIBase32: Windows 98/Me/ 2000/XP
	Desktop or
	Notebook PC	Any 32-bit Windows operating system	USB-ADP-PC
	via Serial		(not included)
	Port

	Handheld PC	Windows CE 2.11 or higher	USB-ADP-H
	via Serial Port		(not included)

Description of Part

Cable that connects from USB port on HR4000 to USB port on the computer

Adapter block that enables connection from serial port on HR4000 to serial port on desktop or notebook PC; comes with 5 VDC power supply (required when connecting to serial port)

Adapter block that enables connection (with standard 9-pin serial cable) from serial port on HR4000 to serial port on handheld PC; comes with 5 VDC power supply (required when connecting to serial port)

Breakout Box

Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the signals from their 30-pin port to an array of standard connectors and headers, enabling easy access to a variety of features found in Ocean Optics’ HR4000 Spectrometer. In addition to the accessory connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows custom circuitry to be prototyped on the board itself.

210-00000-000-02-0908

3

1: Introduction

Note

The HR4000 Breakout Box is compatible with HR4000 Spectrometers with Revision B or greater. HR4000 Spectrometers with serial numbers beginning with HR4A are not compatible with the HR4000 Breakout Box.

Shipment Components

The following information and documentation ships with the HR4000 Spectrometer:

Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice arrives separately). It lists all items in the order, including customized components in the spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes the shipping and billing addresses, as well as any items on back order.

Wavelength Calibration Data Sheet

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your spectrometer. Your spectrometer operating software reads this calibration data from your spectrometer when it interfaces to a computer via the USB port. Any other interface requires that you manually enter the calibration data in OOIBase32 (select Spectrometer | Configure | Wavelength Calibration tab). See the OOIBase32 documentation for more information (refer to Product-Related Documentation for instructions on accessing OOIBase32 documentation).

Note

Please save the Wavelength Calibration Data Sheet for future reference.

Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Resources CD. This disc contains software, operating instructions, and product information for all Ocean Optics software, spectrometers, and spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources CD.

With the exception of OOIBase32 Spectrometer Operating Software, all Ocean Optics software requires a password during the installation process. You can locate passwords for the other software applications on the back of the Software and Technical Resources CD package.

4	210-00000-000-02-0908