Ocean Optics MMS Raman User Manual

MMS Raman Spectrometer

A Product

Installation and Operation Manual

Document Number 000-40000-02-0906

Offices:

E-mail: [e-mail] (General sales inquiries)

Ocean Optics™, Inc.

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]

Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

Ocean Optics B.V. (Europe)

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

F ax 31-(0)26-3190505

[e-mail] (European sales inquiries)
[e-mail] (Questions about orders)
[e-mail] (Technical support)

A

Ocean Optics offers the most comprehensive, innovative and high-quality line of modular spectroscopy
tools in the world. Centice is an essential and valuable partner in this enterprise. We have partnered with
Centice because they are committed to the same goals of innovation and quality that inspire us here at
Ocean Optics. As always, Ocean Optics conducts its business in an open, honest and technically available
fashion. We invite you to contact us at Ocean Optics, Inc. (see front cover for contact information) or
Centice with any technical questions, comments, or applications inquiries. Centice can be contacted at the
following location:

Centice Corporation

4020 Stirrup Creek Dr, Suite 115
Durham, NC 27703
Phone: [phone]
Fax: [phone]
Web: http://www.centice.com

Copyright © 2001-2006 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

Microsoft, Windows, Windows 95, Windows 98, Windows Me, Windows NT, Windows 2000, Windows XP and Excel are either registered
trademarks or trademarks of Microsoft Corporation.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.

Table of Contents

About This Manual .......................................................................................................... iii

Document Purpose and Intended Audience.............................................................................. iii

What’s New in This Document .................................................................................................. iii

Document Summary.................................................................................................................. iii

Product-Related Documentation ............................................................................................... iv

Upgrades......................................................................................................................... iv

Laser Safety ....................................................................................................................iv

Chapter 1: Installation ........................................................................5

Introduction...................................................................................................................... 5

Applications ............................................................................................................................... 5

About SpectraSuite.................................................................................................................... 6

Features..................................................................................................................................... 6

Parts Included ................................................................................................................. 6

Other Equipment Needed..........................................................................................................6

Installation Procedure...................................................................................................... 7

Chapter 2: Operating the Spectrometer............................................9

Sample Placement .......................................................................................................... 9

Acquiring Spectra ............................................................................................................ 10

Appendix A: Specifications................................................................15

Appendix B: Introduction to Multimodal Multiplex Sampling.........17

Introduction...................................................................................................................... 17

Theory of Operation ........................................................................................................ 18

Coded Aperture ............................................................................................................... 18

Coded Aperture ............................................................................................................... 19

Multiplex (SNR) Advantage .......................................................................................................19

Multimodal (Etendue) Advantage .............................................................................................. 19

Imaging Aspect of MMS ............................................................................................................19

MMS and Conventional Multiplex Spectroscopy....................................................................... 20

Performance Comparison ............................................................................................... 20

Etendue ..................................................................................................................................... 20

000-40000-000-02-0906 i

Table of Contents

Signal to Noise Ratio ................................................................................................................. 22

Measuring in Scattering Media.................................................................................................. 23

Conclusion....................................................................................................................... 23

Appendic C: Remote Interlock...........................................................25

Index .....................................................................................................................27

ii 000-40000-02-0906

About This Manual

Document Purpose and Intended Audience

This document provides you with installation information to get your system up and running. It also
contains an in-depth discussion of Multimodal Sampling technology.

What’s New in This Document

This version of the MMS Raman Spectrometer Installation and Operation Manual adds clarification to
the Acquiring Spectra

Document Summary

Chapter Description

procedure.

Chapter 1: Installation Provides a list of system components, and

operating requirements. Also contains instructions
for installing the MMS Raman spectrometer.

Chapter 2: Operating the Spectrometer Contains instructions for using the MMS Raman

spectrometer.

Appendix A: Specifications Provides product specifications for the MMS

Raman spectrometer.

Appendix B: Introduction to Multimodal

Multiplex Sampling

Appendix C: Remote Interlock Contains information on the Remote Interlock

Contains a whitepaper with technical information
describing the operation of the MMS Raman
spectrometer.

feature.

000-40000-000-02-0906 iii

About This Manual

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.

• Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/spectrasuite.pdf

You can also access operating instructions for Ocean Optics products on the Software and Technical
Resources CD included with the system.

Engineering-level documentation is located on our website at Technical → Engineering Docs.

.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

Laser Safety

Although the MMS Raman Spectrometer is laser-safe, we advise that laser safety eyewear must be worn
while operating the system. The MMS Raman uses a Class 3b laser that can cause serious eye injury. The
unit contains a remote interlock feature that deactivates the laser when a plug is inserted (see Remote

Interlock

Optics

for more information). Safety goggles are available for use with the system. Contact an Ocean

Applications Scientist for details.

iv 000-40000-000-02-0906

Chapter 1

Installation

Introduction

The Centice Multimodal Multiplex Sampling (MMS) Raman spectrometer provides significantly higher
measurement sensitivity than a conventional grating based slit entrance spectrometer due to its unique
multimodal multiplex sampling (MMS) technology. An MMS-based spectrometer samples nearly 1,000
optical channels simultaneously through a large area coded aperture – instead of a slit – then applies
proprietary algorithms to precisely construct the spectral content of a source. With MMS technology, both
high resolution and high light throughput can be achieved simultaneously in a single shot measurement.
SpectraSuite software is available with this spectrometer to aid you with your spectral analysis.

The Centice Raman spectrometer is designed to be easy to use and operate with minimal set-up. Follow
the detailed step by step instructions described in this chapter to install the spectrometer.

Applications

MMS-based instruments are ideal for measuring weak, scattering and diffuse sources and samples
because the spectrometer can collect and process far more light through its wide area aperture, without
affecting spectral resolution.

Key applications include material inspection, identification of unknown materials, and quantitative
analysis of both intermediates and final products in the chemical and pharmaceutical industries. Any
material that is Raman-active and fits within the sample chamber of the instrument can be measured.
Typical samples include powders, liquids and polymers. The system is also ideal for teaching and
research applications in colleges and universities.

The MMS system uses a wide-area aperture that cannot be illuminated effectively with a fiber input. The
aperture should be slightly overfilled to obtain the best spectrum. The wide-area aperture and grating
cannot be changed. The MMS aperture and grating are designed specifically for the detector. For fiber­based Raman analysis, consider our

000-40000-000-02-0906 5

QE65000 Spectrometer or the Raman Systems R-3000.

1: Installation

About SpectraSuite

SpectraSuite is a completely modular, Java-based spectroscopy software platform that operates on
Windows, Macintosh and Linux operating systems. The software can control any Ocean Optics USB
spectrometer and device, as well as any other manufacturer’s USB instrumentation using the appropriate
drivers. SpectraSuite is a user-customizable, advanced acquisition and display program that provides a
real-time interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to
perform spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments.

Features

• Novel Multimodal Multiplex Spectroscopy (MMS)

-1

• Spectral resolution of ~4 cm

• Spectral range of 220-2000 cm

• Integration times ranging from 50 ms-100 seconds

• Sample holder for cuvettes and test tubes

• Rapid analysis, with no sample preparation

-1

Parts Included

• Digital Raman Spectrometer with integrated sample holder (holds up to 10-mm cuvettes and test

tubes)

• Power supply for the Spectrometer CCD Camera

• Key for turning the laser on/off

• Removable Remote Interlock plug to automatically disable the laser (see Remote Interlock

more information)

• USB cable to interface to the PC

• Ocean Optics Software and Technical Resources CD with this user manual

Caution

You must first install the software before you power up the spectrometer.

Other Equipment Needed

In addition to the parts you received with your spectrometer, you will need the following equipment to
run your experiments:

• Ocean Optics’ SpectraSuite Spectrometer Operating Software

• A computer to run the software. See the SpectraSuite Spectrometer Operating Software

Installation and Operation Manual for SpectraSuite system requirements.

• Safety goggles (such as the R-2001-GL goggles from Ocean Optics)

for

6 000-40000-000-02-0906

1: Installation

Installation Procedure

Before you power up your Raman spectrometer, you must install the software. See the SpectraSuite
Spectrometer Operating Software Installation and Operation Manual for software installation

instructions. See Product-Related Documentation

for more information on accessing this document.

Procedure

►

1. Install SpectraSuite on the destination computer. See the SpectraSuite Spectrometer Operating

Software Installation and Operation Manual for information on SpectraSuite.

2. Connect the camera power supply to the power interface of the device, and plug the power supply

into an AC outlet. All functions of the spectrometer are operational when it is powered on except
for the laser.

3. Locate the USB cable provided with the spectrometer. Connect one end of the USB cable to the

USB interface of the spectrometer camera and the other end into the computer. Your computer
system should detect the new hardware. Follow the instructions for your computer system for it to
recognize the new hardware device.

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