Ocean Optics Maya2000Pro VIS-NIR User Manual

[e-mail]

(Technical support)

Maya2000, Maya 2000Pro, and
Maya2000Pro VIS-NIR Spectrometers

Install ation and Operation Manual

Document Number 020-00000-000-02-201207

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

000-00000-000-02-A

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)

Additional
Offices:

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
200336 PRC

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics B.V. (Europe)

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Copyright © 2009 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, s tored in a retrieval system, or transmitted, by any means, electroni c,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold a s part of an order and subject to th e condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circula ted without the prior consen t of Ocean Optics, Inc. in any form of binding or cover other th an that in which it is published.

Trademarks

All products and services herein are the t r ademarks, ser v ice marks, registered t rademarks or registered service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as comp l ete and as accu rate as possib l e, but no warra nty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contain ed in this manual.

Table of Contents

About This Manual ......................................................................................................... iii

Document Purpose and Intended Audience .............................................................................. iii

Document Summary .................................................................................................................. iii

Product-Related Documentation ............................................................................................... iii

Upgrades ....................................................................................................................... iv

Warranty ........................................................................................................................ iv

Service .......................................................................................................................... iv

Chapter 1: Introduction ..................................................................... 1

Product Overview .......................................................................................................... 1

Models ........................................................................................................................... 2

Features ........................................................................................................................ 2

System Requirements ................................................................................................... 3

EEPROM Utili zat ion .................................................................................................................. 3

About SpectraSuite Soft ware .................................................................................................... 3

Sampling System Overview....................................................................................................... 4

How Sampling Works ............................................................................................................ 4

Modular Sampling Accessories ............................................................................................. 4

Interface ........................................................................................................................ 4

Shipment Components .................................................................................................. 5

Other Accessories Available .......................................................................................... 5

Breakout Box ............................................................................................................................. 6

Chapter 2: Installing the Maya2000 Series Spectrometers ............. 7

Overview ....................................................................................................................... 7

Maya2000 Series Installation ......................................................................................... 7

Configuring the Maya2000 Series Spectrometers .......................................................... 8

Connect Spectroscopic Accessories .............................................................................. 8

020-00000-000-02-201207 i

Table of Contents

Chapter 3: Troubleshooting .............................................................. 9

Overview ....................................................................................................................... 9

Maya2000 Series Spectrometers Connected to Computer Prior to Software Installation 9

Windows Operating Systems .................................................................................................... 9

Remove the Unknown Device from Windows Device Manager ........................................... 9

Remove Improperly Installed Files ........................................................................................ 10

Mac Operating Systems ............................................................................................................ 10

Linux Operating Systems .......................................................................................................... 11

Appendix A: Calibrating the Wavelength of t he Maya2000 Series

Spectrometers .................................................................................... 13

Overview ....................................................................................................................... 13

About Wavelength Calibration ....................................................................................... 13

Calibrating the Spectrometer ......................................................................................... 14

Preparing for Calibration ............................................................................................................ 14

Calibrating the Wavelength of the Spectrometer ...................................................................... 14

Saving the New Calibration Coefficients: USB Mode ..................................................... 16

Appendix B: Specifications ............................................................... 18

Overview ....................................................................................................................... 18

How the Maya2000 Series Works .................................................................................. 18

Maya2000 Series Components Table ....................................................................................... 19

Maya2000 Series Spectrometers Specifications ............................................................ 20

CCD Detector Specifications ..................................................................................................... 20

Maya2000 Series Spectrometer Specifications......................................................................... 21

System Compatibility ..................................................................................................... 22

30-Pin Accessory Connector Pinout .............................................................................. 22

30-Pin Accessory Connector Pinout Diagram ........................................................................... 23

30-Pin Accessory Connector – Pin Definitions and Descriptions .............................................. 23

30-Pin J2 Accessory Connector - Part Num ber s....................................................................... 25

Index ................................................................................................... 27

ii 020-00000-000-02-201207

About This Manual

Document Purpose and Intended Audience

This document provides the user of a Maya2000 Series Spectrometer with instructions for setting up,
calibrating and performing experiments with their spectrometer.

What’s New in this Document

This version of the Maya2000 and Maya2000Pro and Maya2000Pro VIS-NIR Installation and Operation
Manual updates the models offered.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: Installing the

Maya2000 Series

Chapter 3: Troubleshooting

Appendix A: Calibrating the

Wavelength of the Maya2000
Series

Appendix B: Specifications

Contains descriptive information about the Maya2000 Series
Spectrometers and how sampling works. It also provides a list of
system requirements, interface options, and shipment
components.

Provides installation and configuration instructions.

Contains recommended steps to isolate and correct common
problems.

Provides instructions for calibrating the Maya2000 Series
Spectrometers.

Contains technical specifications and connector pinouts for the
Maya2000 Series Spectrometers.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com

document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.

. Select Technical → Operating Instructions, then choose the appropriate

• Detailed instructions for SpectraSuite Spectrometer Operating Software is located at:

http://www.oceanoptics.com/technical/SpectraSuite.pdf.

020-00000-000-02-201207 iii

About This Manual

• Detailed instructions for the Breakout Box are located at:

http://www.oceanoptics.com/technical/HR4_breakout.pdf

• Detailed instructions for external triggering are located at:

http://www.oceanoptics.com/technical/external

Engineering-level documentation is located on our website at Technical → Engineering Docs.
You can also access operating instructions for Ocean Optics products from the Software and Technical

Resources CD that ships with the product.

-triggering.pdf.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first conta ct Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

Warranty

Our 3-Year Warranty, currently the best in the industry, covers all Ocean Optics miniature fiber optic

spectrometers – regardless of the application – from manufacturing defects. It ensures you of the highest
level of craftsmanship and reliability for years to come. Our warranty information is located at

http://www.oceanoptics.com/corporate/3-year%20warranty%20certificate.pdf.

Service

For additional peace of mind, we offer an Annual Service Package (ASP) to maintain your
scientific investment.

and privileged customer status plan for all our MAYA2000 series spectrometers. More information on

available ASPs is located at
Contact us to learn more about these great service packages.

This plan includes yearly wavelength calibration, preventive maintenance service

http://www.oceanoptics.com/Services/servicepackages.asp

iv 020-00000-000-02-201207

Chapter 1

Introduction

Product Overview

The Ocean Optics Maya2000 Series Spectrometers have been engineered specifically for low light-level,
UV-sensitive and other scientific applications such as fluorescence, DNA sequencing and Raman
spectroscopy. These next-generation spectrometers have great quantum efficiency, high dynamic range,
extreme sensitivity and excellent deep-UV (<190 nm) response.

Ocean Optics Maya2000Pro High-Sensitivity Fiber Optic Spectrometer

The Maya2000 Series Spectrometers feature the Hamamatsu FFT-CCD back-thinned detector, which
offers excellent performance characteristics. Because of their great native UV-response, FFT-CCD
detectors do not require UV-sensitive coatings, eliminating batch-to-batch variations. Other performance
advantages of this detector include great signal-to-noise characteristics, low dark current and good signal
processing speed.

The Maya2000 Series’ onboard module has 10 user-programmable digital I/O lines for interfacing to
other equipment; and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp,
stop/start a process, and send a message/alarm during the spectrometer’s integration period. The
spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration
coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.

020-00000-000-02-201207 1

1: Introduction

TheMaya2000 Series’ high-speed electronics have been designed for considerable flexibility in
connecting to various modules and external interfaces, including PCs, PLCs and other embedded
controllers, through USB 2.0 communications. Its USB 2.0 interface enables full spectral scans into
memory every 7 milliseconds.

The Maya2000 Series Spectrometers operate via USB interface. External triggering is not supported by
the Maya2000 Series Spectrometers.

Models

The Maya2000 Series Spectrometers consist of three models, the Maya2000 and the Maya2000 Pro, and
the Maya2000Pro VIS-NIR. The Maya2000 has been discontinued. While these spectrometers have
similar performance for most parameters, the Maya2000 has slightly faster readout time, and the Pro
offers better dynamic range and signal-to-noise. The May2000Pro VIS-NIR offers high sensitivity in the
NIR range while the Maya2000Pro offers high sensitivity in the UV range. See

Maya2000 Series

Spectrometers Specifications for a side-by-side comparison.

Features

• Hamamatsu S9840 (Maya2000), Hamamatsu S10420 (Maya2000Pro), or Hamamatsu S11510

(Maya2000Pro VIS-NIR) Detector

• Peak QE: >90% for Maya2000; 75% for Maya2000 Pro; 85% for Maya2000Pro VIS-NIR

• Back-thinned for good UV sensitivity

• MPP operation for low noise

• Spectrometer Design

• Symmetrical Crossed Czerny Turner

• 101mm focal length

• 14 grating options, including the HC-1 composite grating for coverage from 175-1100 nm

(additional charge)

• 6 slit widths

• Electrical Performance

• 16 bit, 500KHz A/D converter

• Integration times: 6ms to 10sec for Maya2000; 6ms to 5sec for Maya2000 Pro

• Embedded microcontroller allows programmatic control of all operating parameters and

standalone operation

• USB 2.0 480Mbps (high-speed) & 12Mbps (full speed)

• Multiple communication standards for digital accessories (SPI, I2C)

• Onboard Pulse Generator

• 2 programmable strobe signals for triggering other devices

• Software control of nearly all pulse parameters

• Onboard GPIO

• 10 user-programmable digital I/Os

2 020-00000-000-02-201207

1: Introduction

• EEPROM storage for

• Wavelength Calibration Coefficients

• Linearity Correction Coefficients

• Absolute Irradiance Calibration (optional)

• Plug-n-play interface for PC applications

• 30-pin connector for interfacing to external products

• CE certification

System Requirements

You can use the Maya2000 Series’ USB connectivity with any computer that meets the requirements for
the spectrometer operating software being used (Windows 98/Me/2000/XP, Mac OS X and Linux). See

About SpectraSuite Software

EEPROM Utilization

An EEPROM memory chip in each Maya2000 Series contains wavelength calibration coefficients,
linearity coefficients, and a serial number unique to each individual spectrometer. The OOI software
application reads these values directly from the spectrometer, enabling the ability to “hot-swap”
spectrometers between computers without entering the spectrometer coefficients manually on each
computer.

.

About SpectraSuite Software

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation

).

020-00000-000-02-201207 3

1: Introduction

Sampling System Overview

How Sampling Work s

Ocean Optics components function in a sampling system as follows:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

5. The spectrometer measures the amount of light and transforms the data collected by the

spectrometer into digital information.

6. The spectrometer passes the sample informatio n to O OI software.

7. OOI software compares the sample to the reference measurement and displays processed spectral

information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spec tro scop ic ac cesso ri es for use with the Maya2000 Series. Most
of our spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing
the sampling system components is as easy as unscrewing a connector and replacing an accessory.

Interface

The Maya2000 Series has a USB connector, enabling you to connect the spectrometer to a desktop or
notebook computer via a USB port. SpectraSuite software is available to operate your spectrometer for an
additional charge.

Computer
Interface

Computer via
USB Port

Operating System Requirements Part Needed Description of Part

SpectraSuite: Windows2000/XPfor
PC, OS X version 10.0 or later for
Mac, or Red Hat 9 or later, Fedora
(any version), Debian 3.1 (Sarge),
and SUSE (9.0 or later) for Linux

USB-CBL-1
(included)

Cable that connects from USB port
on Maya2000 Series to USB port on
desktop or notebook PC

4 020-00000-000-02-201207

1: Introduction

Shipment Components

 Maya2000 Series Spectrometer

The following information and documentation also ships with the Maya2000 Series Spectrometer:

 Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice

arrives separately). It lists all items in the order, including customized components in the

spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes

the shipping and billing addresses, as well as any items on back order.

 Wavelength Calibration Data Sheet

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information

unique to your spectrometer. Your spectrometer operating software reads this calibration data

from your spectrometer when it interfaces to a computer via the USB port.

Note

Please save the Wavelength Calibration Data Sheet for future reference.

 Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Resources CD. This disc contains software,

operating instructions, and product information for all Ocean Optics software, spectrometers, and

spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these

files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources

CD.

All Ocean Optics software requires a password during the installation process. You can locate

passwords for the other purchased software applications on the back of the Software and

Technical Resources CD package.

Other Accessories Available

Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.

 Fibers
 Light Sources
 Integrated Sampling Systems
 Cuvettes
 Filter Holders
 Lithium Ion Battery Pack
 HR4-BREAKOUT Breakout Box

020-00000-000-02-201207 5

1: Introduction

Breakout Box

Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the
signals from their 22-pin port to an array of standard connectors and headers, enabling easy access to a
variety of features found in Ocean Optics’ Maya2000 Series Spectrometer. In addition to the accessory
connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows
custom circuitry to be prototyped on the board itself.

6 020-00000-000-02-201207

Chapter 2

Installing the Maya2000 Series

Spectrometers

Overview

You must install the operating software application prior to connecting your Maya2000 Series
Spectrometer to a computer. The Ocean Optics SpectraSuite spectrometer operating software in st al ls the
drivers required fo r the Maya2000 Series spectrometer installation. If you do not install the software first,
the system will not properly recognize the Maya2000 Series.

If you have already connected the Maya2000 Series to the computer prior to installing the operating
software, consult Chapter 3: Troubleshooting
installation.

Maya2000 Series Installation

This section contains instructions for connecting the Maya2000 Series Spectrometer to a computer. To
connect the Maya2000 Series Spectrometer to a computer via the USB port, the computer must be
running a Windows 98/Me/2000/XP, Mac OS X or Linux operating system.

for information on correcting a corrupt Maya2000 Series

►

Procedure

Follow the steps below to connect the Maya2000 Series to a computer via the USB port:

1. Install the spectrometer operating software on the destination computer.

2. Locate the USB cable (USB-CBL-1) provided with the Maya2000 Series Spectrometer.

3. Insert the square end of the cable into the side of the spectrometer.

4. Insert the rectangular end of the cable into the USB port of the computer.

If you installed SpectraSuite prior to connecting the spectrometer, SpectraSuite installs the spectrometer
drivers. If the drivers do not successfully install (or if you connected the spectrometer to the com puter
before installing SpectraSuite), consult Chapter 3: Troubleshooting

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

020-00000-000-02-201207 7

.

2: Installing the Maya2000 Series

Note the spectrometer(s) that you have installed are listed in the Data Sources pane of your SpectraSuite
software.

Once you install the software and hardware, and establish your sampling system, you are ready to take
measurements.

Configuring the Maya2000 Series Spectrometers

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If the graph does not automatically appear, select File | New | Spectrum Graph. If you allow
light into the spectrometer, the graph trace should rise with increasing light intensity. This means the
software and hardware are correctly insta ll ed.

Connect Spectroscopic Accessories

To find operating instructions for Maya2000 Series-compatible products (such as lig ht sourc es, sampling
chambers, and probes), consult the Software and Technical Resources CD or the Ocean Optics website at

http://www.oceanoptics.com/technical/operatinginstructions.asp.

8 020-00000-000-02-201207

Chapter 3

Troubleshooting

Overview

The following sections contain information on troubleshooting issues you may encounter when using the
Maya2000 Series Spectrometer.

Maya2000 Series Spectrometers Connect ed to Computer Prior to Software Installation

Windows Operating Systems

If you connected your Ocean Optics Maya2000 Series device to the computer prior to installing your
SpectraSuite spectrometer operating software application on a Windows platform, you may encounter
installation issues that you must correct before your Ocean Optics device will operate properly.

Follow the applicable steps below to remove the incorrectly installed device, device driver, and
installation files.

Note

If these procedures do not correct your device driver problem, you must obtain the

Correcting Device Driver Issues document from the Ocean Optics website:

http://www.oceanoptics.com/technical/engineering/correctingdevicedriverissues.pdf

Remove the Unknown Device from Windows Device Man ager

► Procedure

1. Open Windows Device Manager. Consult the Windows operating instructions for your computer

for directions, if needed.

2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+"

sign to the imme diate le ft.

.

020-00000-000-02-201207 9

3: Troubleshooting

Note

Improperly installed USB devices can also appear under the Universal Serial Bus

Controller option. Be sure to check this location if you cannot locate the unknown device.

3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown

Device listing and select the Uninstall or Remove option.

4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown

Device. Click the OK button to confirm the device removal.

5. Disconnect the Maya2000 Series from your computer.

6. Locate the section in this chapter that is appropriate to your operating system and perform the

steps in the following Remove Improperly Installed Files

section.

Remove Improperly Installed Files

► Procedure

1. Open Windows Explorer.

2. Navig ate to the Windows | INF directory.

Note

If the INF directory is not visible, you must disable the Hide System Files and Folders

and Hide File Extensions for Known File Types options in Windows Folder Options.

Access Windows Folder Options from Windows Explorer, under the Tools | Folder

Options menu selection.

3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows

2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory.

4. Navig ate to the Windows | System32 | Drivers directory.

5. Delete the EZUSB.SYS file.

6. Reinstall your Ocean Optics application and reboot the system when prompted.

7. Plug in the USB device.

The system is now able to locate and install the correct drivers for the USB device.

Mac Operating Systems

Since there are no device files for the Maya2000 Series Spectrometer in a Mac operating system, you
should not encounter any problems if you installed the spectrometer before the SpectraSuite software.

10 020-00000-000-02-201207

3: Troubleshooting

Linux Operating Systems

For Linux operating systems, all you need to do is install the SpectraSuite software, then unplug and
replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged users
permission to use newly connected hardware. There isn’t any long-term harm to plugging in the device
before installing the software.

020-00000-000-02-201207 11

3: Troubleshooting

12 020-00000-000-02-201207

Appendix A

Calibrating the Wavelength of

the Maya2000 Series

Spectrometers

Overview

This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectromete r s w ill drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
Maya2000 Series.

About Wavelength Calibration

You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynom ial:

λ

= I + C1 p + C2 p2 + C3 p

p

Where:

λ

= the wavelength of pixel p

I = the wavelength of pixel 0

= the first coefficient (nm/pixel)

C

1

= the second coefficient (nm/pixel2)

C

2

= the third coefficient (nm/pixel3)

C

3

You will be calculating the value for I and the three Cs.

3

020-00000-000-02-201207 13

A: Calibrating the Wavelength of the Maya2000 Series

Calibrating the Spectrometer

Preparing for Calibration

To recalibrate the wavelength of your spectrometer, you need the following components:

• A light source capable of producing spectral lines

Note

Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an

HG-1, you need a light source that produces several (at least 4-6) spect ral lines in th e

wavelength region of your spectrometer.

• A Maya2000 Series spectrometer

• An optical fiber (for spectrometers without a built-in slit, a 50-

• A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-

order linear regressions

µm fiber works best)

Note

If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak

and AnalysisTookPak-VBA.

Calibrating the Wavelength of the Spectrometer

► Procedure

Perform the steps below to calibrate the wavelength of the spectrometer:

1. Place the spectrometer operating software into Scope mode and take a spectrum of your light
source. Adjust the integration time (or the A/D conversion frequency) until there are several
peaks on the screen that are not off-scale.

2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.

3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.

4. Use the spreadsheet program or calculator to create a table like the one shown in the following
figure. In the first column, place the exact or true wavelength of the spectral lines that you used.

In the second column of this worksheet, place the observed pixel number. In the third column,
calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.

14 020-00000-000-02-201207

A: Calibrating the Wavelength of the Maya2000 Series

Independent

Dependent

Values Computed

Variable

Variables

True Wavelength (nm) Pixel # Pixel # 2 Pixel # 3

253.65

296.73

302.15

313.16

334.15

365.02

404.66

407.78

435.84

546.07

576.96

579.07

696.54

706.72

727.29

738.40

751.47

175
296
312
342
402
490
604
613

694
1022
1116
1122
1491
1523
1590
1627
1669

30625
87616

97344
116964
161604
240100
364816
375769
481636

1044484
1245456
1258884
2223081
2319529
2528100
2647129
2785561

5359375
25934336
30371328
40001688
64964808

117649000
220348864
230346397

334255384
1067462648
1389928896
1412467848
3314613771
3532642667
4019679000
4306878883
4649101309

Predicted

Wavelength

from the Regr ession

Output

253.56

296.72

302.40

313.02

334.19

365.05

404.67

407.78

435.65

546.13

577.05

579.01

696.70

706.62

727.24

738.53

751.27

Difference

0.09

0.01

-0.25

0.13

-0.05

-0.04

-0.01

0.00

0.19

-0.06

-0.09

0.06

-0.15

0.10

0.06

-0.13

0.19

5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the
spreadsheet program, find the functions to perform linear regressions.

• If using Quattro Pro, look under Tools | Advanced Math

• If using Excel, look under Analysis ToolPak

6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number
squared, and the pixel number cubed as the independent variables (X). After executing the
regression, you will obtain an output similar to the one shown below. Numbers of importance are
noted.

Regression Statistics

Multiple R 0.999999831
R Square 0.999999663 R Squared
Adjusted R Square 0.999999607
Standard Error 0.125540214
Observations 22

Intercept

Coefficients Standard Error

Intercept 190.473993 0.369047536 First coefficient
X Variable 1 0.36263983 0.001684745
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10 Second coefficient

Third coefficie n t

020-00000-000-02-201207 15

A: Calibrating the Wavelength of the Maya2000 Series

7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.

Keep these values at hand.

Saving the New Calibration Coefficients: USB Mode

Ocean Optics programs wavelength calibration coefficients unique to each Maya2000 Series
Spectrometer onto an EEPROM memory chip in the spectrometer.

You can overwrite old calibration coefficients on the EEPROM using the Maya2000 Series Spectrometer
via the USB port.

►

Procedure

To save wavelength calibration coefficients using the USB mode, perform the following steps:

1. Ensure that the Maya2000 Series is connected to the PC and that you have closed all other
applications.

2. Point your browser to http://www.oceanoptics.com/technical/softwaredownloads.asp
scroll down to Microcode. Select USB EEPROM Program me r.

3. Save the setup file to your computer.

4. Run the Setup.exe file to install the software. The Welcome sc reen appe ar s.

5. Click the Next button. The Destination Location screen appears.

6. Accept the default installation location, or click the Browse button to specify a directory. Then,
click the Next button. The Program Manager Group screen appears.

7. Click the Next button. The Start Installation screen a ppears.

8. Click the Next button to begin the installation. Once the installation finishes, the Installation
Complete screen appe ars.

9. Click the Finish button and reboot the computer when prompted.

10. Navigate to the USB EEPROM Progra mmer from the Start menu and run the software.

11. Click on the desired spectrometer displayed in the left pane of the USB Programmer screen.

12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new valu es acquired in Steps 5 and 6 of the

Wavelength of the Spectrometer section in this appendix.

13. Repeat Step 12 for all of the new values.

14. Click on the Save All Values button to save the information, and then Exit the USB Programmer
software.

Calibrating the

and

The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
Maya2000 Series Spectrometer.

16 020-00000-000-02-201207

A: Calibrating the Wavelength of the Maya2000 Series

020-00000-000-02-201207 17

Appendix B

Specifications

Overview

This appendix contains information on spectrometer operation, specifications, and system compatibility.
It also includes accessory connector pinout diagrams and pin-specific information.

How the Maya2000 Series Works

The optical bench has no moving parts that can wear or break; all the components are fixed in place at the
time of manufacture.

020-00000-000-02-201207 18

B: Specifications

Maya2000 Series Components Table

Ocean Optics permanently secures all components in the Maya2000 Series Spectrometers at the time of
manufacture. Only Ocean Optics technicians can replace interchangeable components, where noted.

Item Name Description

1

2 Slit

3 Filter

4

5 Grating

SMA
Connector

Collimating
Mirror

Secures the input fiber to the spectrometer. Light from the input fiber enters the
optical bench through this connector.

A dark piece of material containing a rectangular aperture, which is mounted directly
behind the SMA Connector. The size of the aperture regulates the amount of light
that enters the optical bench and controls spectral resolution.

You can also use the Maya2000 Series Spectrometer without a Slit. In this
configuration, the diameter of the fiber connected to the spectrometer determines
the size of the entrance aperture.

Only Ocean Optics technicians can change the Slit.
Restricts optical radiation to pre-determined wavelength regions. Light pas s es

through the Filter before entering the optical bench. Both bandpass and longpass
filters are available to restrict radiation to certain wavelength regions.

Only Ocean Optics technicians can change the Filter.
Focuses light entering the optical bench towards the Grating of the spectrometer.

Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,
and then reflects off the Collimating Mirror onto the Grating.

Diffracts light from the Collimating Mirror and directs the diffracted light onto the
Focusing Mirror. Gratings are available in different groove densities, allowing you to
specify wavelength coverage and resolution in the spectrometer.

Only Ocean Optics technicians can change the Grating.

6

7

8

020-00000-000-02-201207 19

Focusing
Mirror

Detector
with OFLV
Filter

Back­thinned
Area
Detector

Receives light reflected from the Grating and focuses the light onto the CCD
Detector or L2 Detector Collection Lens (depending on the spectrometer
configuration).

Eliminates second-order effects and is used with an HC-1 Grating in a 200-950 nm
wavelength system in a Maya2000 Series spectrometer.

Provides 90% (Maya200) or 75% (Maya2000 Pro) quantum efficiency and bins
pixels in a vertical column to acquire light form the entire height of the
spectrometer’s slit image. This improves light collection and signal-to-noise
significantly. This 2D area detector is back-thinned (back-illuminated) and does not
require the detector upgrade that is normally applied to other detectors.

Only Ocean Optics technicians can add or remove the Detector.

B: Specifications

Maya2000 Series Spectrometers Specifications

The following sections provide specification information for the CCD detector in the Maya2000 Series
spectrometers, as well as for each model Maya2000 Series Sp e ctrometer itself.

CCD Detector Specifications

Specification Maya2000 Maya2000 Pro Maya200Pro VIS-NIR

Detector

Thermoelectric
cooling

Number of pixels

Spectral range

Pixel size 14 µm square
Pixel well depth 130 Ke- 200 Ke­Column height 196 µm square 896 µm square
Detector active area

(mm)
Quantum efficiency:

Peak QE
QE @ 250 nm

Hamamatsu S9840,
back-thinned FFT-CCD

All: 2080 x 20
Active: 2048 x 14

200-1100 nm with window, Deep UV option available (150nm). Deep UV option
includes purge port and window removal.

175-1100 nm with HC1 grating.

28.672 horizontal x 0.196
vertical

>90%
55%

Hamamatsu S10420,
back-thinned FFT-CCD

No

All: 2068 x 70
Active: 2048 x 64

28.672 horizontal x 0. 8 96 ver tical

75% at 600 nm
60%

Hamamatsu S11510,
back-thinned FFT-CCD

85% at 700 nm

20 020-00000-000-02-201207

B: Specifications

Quantum Efficiency of S9840 Detector Quantum Efficiency of S10420 and S11510 Detectors

Maya2000 Series Spectrometer Specifications

Specification Maya2000 Maya2000 Pro Maya2000Pro VIS-NIR

Dimensions (LxWxH) 148.6 mm (5.85 in.) x 109.3 mm (4.30 in.) x 50.4 mm (1.98 in.)

Weight

Temperature
Operation
Storage

Humidity 0 – 90% noncondensing
Power consumption 500 mA @ 5 VDC
Supply Voltage 4.5 – 5.5 V
Power-up Time ~ 2s depending on code size
Gratings 14 gratings available
Entrance aperture 5, 10, 25, 50, 100 or 200 µm wide slits
Order-sorting filters 6 OF series available

0.96 kg (2.12 lbs.)

–0 °C to +50 °C

–30 °C to +70 °C

Focal length (input) f/4, 101 mm
Optical resolution

(FWHM)

020-00000-000-02-201207 21

Depends on grating and size of entrance aperture

B: Specifications

Specification Maya2000 Maya2000 Pro Maya2000Pro VIS-NIR

Stray light <0.05% at 600 nm; <0.10% at 435 nm
A/D converter 16 bit, 150 kHz+
Dynamic range

Spec
Typical

Signal-to-noise ratio 350:1 450:1
Non-linearity

Uncorrected
Corrected

Fiber optic connector SMA 905 to s ingle-strand optical fiber (0.22 NA)
Integration time 6 ms to 10 seconds 6 ms to 5 seconds
Interfaces USB 2.0

5000:1
8000:1+

~4%
<1.0%

8000:1

12000:1+

~10.0%

<1.0%

System Compatibility

You can use the Maya2000 Series’ USB connectivity with any computer that meets the requirements for
the spectrometer operating software being used (Windows 98/Me/2000/XP, Mac OS X and Linux). See

About SpectraSuite Software

.

30-Pin Accessory Connector Pinout

The Maya2000 Series features a 30-pin Accessory Connector, located on the side of the unit as shown:

22 020-00000-000-02-201207

B: Specifications

Location of Maya2000 Series 30-Pin Accessory Connector

30-Pin Accessory Connector Pinout Diagram

When facing the 30-pin Accessory Connector on the front of the vertical wall of the Maya2000 Series
Spectrometer, pin numbering is as follows:

USB

Port

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

1 3 5 7 9 11 13 15 17 19 21 23 25 27 29

30-Pin Accessory Connector Pinout Diagram

30-Pin Accessory Connector – Pin Definitions and Descriptions

The following table contains information regarding the function of each pin in the Maya2000 Series
Spectrometer’s 30-Pin Accessory Connector:

Pin # Function Input/Output Description

1 N/A N/A Reserved
2 N/A N/A Reserved

020-00000-000-02-201207 23

B: Specifications

Pin # Function Input/Output Description

3 GPIO (2) Input/Output

General purpose software-programmable, digital input/output

(channel number)
4 N/A N/A Reserved
5 Ground Input/Output Ground
6 I2C SCL Input/Output I2C clock signal for communication to other I2C peripherals

7 GPIO (0) Input/Output

General purpose software-programmable, digital input/output

(channel number)
8 I2C SDA Input/Output I2C data signal for communication to other I2C peripherals

9 GPIO (1) Input/Output

10

Ext. Trigger
In

Input TTL input trigger signal

11 GPIO (3) Input/Output

General purpose software-programmable, digital input/output

(channel number)

General purpose software-programmable, digital input/output

(channel number)

Input power pin for Maya2000 Series – When operating via
12 VCC or 5VIN Input or Output

USB, this pin can power other peripherals – Ensure that

peripherals comply with USB specifications (no TEC power)

13 SPI Data Out Output

SPI Master Out Slave In (MOSI) signal for communication to

other SPI peripherals

Input power pin for Maya2000 Series – When operating via
14 VCC or 5VIN Input or Output

USB, this pin can power other peripherals – Ensure that

peripherals comply with USB specifications (no TEC power)

15 SPI Data In Input

16 GPIO (4) Input /Output

SPI Master In Slave Out (MISO) signal for communication to

other SPI peripherals

General purpose software-programmable, digital input/output

(channel number)

TTL output pulse used as a strobe signal – Has a programmable
17 Single Strobe Output

delay relative to the beginning of the spectrometer integration

period

18 GPIO (5) Input/Output

General purpose software-programmable, digital input/output

(channel number)
19 SPI Clock Output SPI clock signal for communication to other SPI peripherals

24 020-00000-000-02-201207

B: Specifications

Pin # Function Input/Output Description

20

21

22 GPIO (6) Input/Output

23 N/A N/A Reserved
24 N/A N/A Reserved

25 Lamp Enable Output

26 GPIO (7) Input/Output

27 Ground Input/Output Ground

28 GPIO (8) Input/Output

29 Ground Input/Output Ground

Continuous
Strobe

SPI Chip
Select

Output

Output

TTL output signal used to pulse a strobe – Divided down from

the master clock signal

SPI Chip/Device Select signal for communication to other SPI

peripherals

General purpose software-programmable, digital input/output

(channel number)

TTL signal driven Active HIGH when the Lamp Enable

command is sent to the spectrometer

General purpose software-programmable, digital input/output

(channel number)

General purpose software-programmable, digital input/output

(channel number)

30 GPIO (9) Input/Output

General purpose software-programmable, digital input/output

(channel number)

30-Pin J2 Accessory Connector - Part Numbers

The part numbers for the 30-pin accessory connector on the Maya2000 Series S p ect rom eter are as
follows:

• The connector is Pak50™ model from 3M Corp. Headed Connector – Part Number

P50–030P1–RR1–TG.

• The mating connector is Part Number P50–030S–TGF.

• Mating the two components requires two 1.27 mm (50 mil) flat ribbon cables (3M 3365 Series is

recommended).

020-00000-000-02-201207 25

B: Specifications

26 020-00000-000-02-201207

Index

3

30-pin accessory connector

diagram, 23
part numbers, 25
pin definitions, 23

A

accessories, 5, 8
Accessories, 4
accessory connector

pinout, 22

Adobe Acrobat Reader, 5

B

breakout box, 6

C

E

EEPROM, 3

F

filter, 19
focusing mirror, 19

G

grating, 19

I

Installation, 7
installed filter, 19
Interface, 4

M

Calibrating , iii, 13
calibration, 13

preparing for, 14
procedure, 14

calibration coefficients

saving in USB mode, 16
CCD, 20
CCD Detector, 19
collimating mirror, 19
compatibility, 22
Components Table, 18

memory chip, 3
mirror, 19

P

packing list, 5
passwords, 5
power supply (external), 21, 22
product-related documentation, iii

D

detector, 20
document

audience, iii

purpose, iii

summary, iii

020-00000-000-02-201207 27

Index

S

Sampling

Accessories, 4

System, 4
service, iv
setup, 7
shipment components, 5
slit, 19
SMA Connector, 19
software

SpectraSuite, 3
Software and Resources Library CD, 5
specifications, 18

detector, 20

spectrometer, 21
specifications, 19
SpectraSuite, 3, 4
spectroscopic accessori es, 8
System Requirements, 3

T

troubleshooting

Linux systems, 11
Mac systems, 10

Troubleshooting, 9

U

upgrades, iv
USB-CBL-1, 4

W

warranty, iv
Wavelength Calibration

about, 13
Wavelength Calibration Data File, 5
Wavelength Calibration Data Sheet, 5

28 020-00000-000-02-201207