Ocean Optics LS-450 User Manual

Blue LED Pulsed Light Source

LS-450

Installation and Operation Manual

Document Number 009-00000-450-02-201105

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

000-00000-000-02-A

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

Additional
Offices:

Regional Headquarters
Maybachstrasse 11
73760 Ostfildern

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District,
Shanghai, PRC. 200336

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics EMEA

Sales and Support Center
Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail

[e-mail]

Copyright © 2011 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical,
photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise
circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

Microsoft, Windows, Windows 95, Windows 98, Windows Me, Windows NT, Windows 2000, Windows XP and Excel are either registered trademarks or
trademarks of Microsoft Corporation.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on
an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the
information contained in this manual.

Important Safety Notices

1. Do not remove or modify any installed safety device on this equipment. Doing so will void your
warranty and create an unsafe operating environment.

2. Dangerous voltages are present in this device. There are NO user serviceable parts inside.

3. Only allow qualified personnel to service this unit.

4. Do not use the unit if it is damaged in any way. Contact your dealer for repair or replacement
information.

009-00000-450-02-201105 A

Important Safety Notices

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Table of Contents

Chapter 1: Introduction..............................................................................1

Product Overview ...............................................................................................................1

Features .............................................................................................................................2

Unpacking the LS-450........................................................................................................2

Chapter 2: LS-450 Standalone Version Set-up and Operation.............1

Overview.............................................................................................................................1

Package Contents ..............................................................................................................1

Connecting the LS-450 Standalone Version .....................................................................1

Connecting for Pulsed Mode Operation .................................................................................... 2

Operating the LS-450 Standalone Version ........................................................................4

Continuous Mode Operation...................................................................................................... 4

Pulsed Mode Operation.............................................................................................................4

Chapter 3:

Overview.............................................................................................................................1

Connecting the R-LS-450 Rack-mount Version with an S2000 Spectrometer .................2

Chapter 4:

Package Contents ..............................................................................................................7

Connecting the USB-LS-450..............................................................................................8

R-LS-450 Rack-mount Set-up and Operation .......................1

S1 Switch................................................................................................................................... 2

Jumper Block 1 (JP1) ................................................................................................................2

Jumper Block 2 (JP2) ................................................................................................................2

Jumper Block 3 (JP3) ................................................................................................................3

R-LS-450 Operation Matrix........................................................................................................ 3

Using Jumper Block 3 (JP3) on the S2000 ............................................................................... 4

Continuous Wave Mode with JP3 on the S2000 ....................................................................... 4

Pulsed Mode with JP3 on the S2000 ........................................................................................ 4

Setting the Integration Time with the R-LS-450 and the S2000................................................ 5

R-LS-450-2 Model .....................................................................................................................6

USB-LS-450 Blue LED Pulsed Light Source.........................7

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Table of Contents

Chapter 5: Specifications ..........................................................................9

LS-450 Series Technical Specifications ............................................................................9

Lamp Specifications .................................................................................................................. 9

Absolute Spectral Output for LS-450 Blue LED Bulbs .............................................................. 9

Pinout Information ..................................................................................................................... 10

USB-LS-450 Specifications................................................................................................10

Temperature Measurement Specifications................................................................................ 10

Power Specifications .................................................................................................................11

USB-LS-450 Pin and Interface Information............................................................................... 11

Appendix A:

Maintenance ...........................................................................13

Bulb Replacement for LS-450 Series Light Sources .........................................................13

Changing a Bulb in the LS-450 ................................................................................................. 13

Changing a Bulb in the R-LS-450.............................................................................................. 14

Troubleshooting..................................................................................................................16

Index ......................................................................................................17

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About This Manual

Document Purpose and Intended Audience

This document provides you with an installation section to get your system up and running.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: LS-450 Standalone Version

Set-up and Operation

Chapter 3: R-LS-450 Rack-mount Set-up

and Operation

Chapter 4: USB-LS-450 Blue LED

Pulsed Light Source

Chapter 5: Specifications

Appendix A: Maintenance

Contains product information for the LS-450 and
information on unpacking.

Contains instructions for setting up and operating the
LS-450.

Contains instructions for setting up and operating the
R-LS-450.

Contains instructions for setting up the USB-LS-450.

Provides product specifications.

Contains instructions for changing the bulb and
troubleshooting information.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the
of the web page.

Search by Model Number field at the bottom

You can also access operating instructions for Ocean Optics products on the

Resources

Engineering-level documentation is located on our website at

009-00000-450-02-201105 iii

CD included with the system.

Technical → Engineering Docs.

Software and Technical

About This Manual

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

iv 009-00000-450-02-201105

Chapter 1

Introduction

Product Overview

The LS-450 Series Blue LED Pulsed Light Sources are compact, low-cost light-emitting diodes that
produce pulsed or continuous spectral output at 470 nm – the blue region – for high-sensitivity emission
fluorescence measurements.

The LS-450 light source can be combined with other sampling optics for a variety of fluorescence
applications.

The LS-450 series consists of the following light sources:

 LS-450 (stand-alone version)

 R-LS-450 (rack-mount version)

 R-LS-450-2 (rack-mount version with 2 LEDs)

 USB-LS-450 (connects directly to USB spectrometers, documented separately)

LS-450 (Standalone version)

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1: Introduction

Features

 Excitation Source. Produces either pulsed or continuous output at 470 nm for high-sensitivity

fluorescence applications

 Software Operation. Lamps can be turned on/off and used in pulsed or continuous mode through

manual or software operation

 Synchronization with Detector Scan Rate. Configure lamp pulse rates via jumper blocks on the

lamp or on the spectrometer

Unpacking the LS-450

► Procedure

1. Unpack your lamp assembly carefully. Although the blue LED lamp is rigidly mounted, dropping
this instrument can cause permanent damage.

2. Inspect the outside of the instrument and make sure that there is no damage. Do not use the
instrument if damage is present. Contact your dealer for repair or replacement information, if
necessary.

3. Use this instrument in a clean laboratory environment (see

Error! Reference source not found.).

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