Nikon TE2000-E, TE2000-U, TE2000-S User Manual

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M314E 04.7.CF.3(1/5)

Inverted Microscope

ECLIPSE TE2000-E

ECLIPSE TE2000-U

ECLIPSE TE2000-S

Instructions

Thank you for purchasing the Nikon products.

This instruction manual is written for the users of the Nikon’s inverted microscopes “ECLIPSE TE2000-E, ECLIPSE

TE2000-U, ECLIPSE TE2000-S” and describes the basic operations of the microscope.

To ensure correct usage, read this manual carefully before operating the instrument.

• It is prohibited to reproduce or transmit th is manual in part or whole without Nikon’s expressed permission.

• The contents of this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, if you note any points that are unclear

or incorrect, contact your nearest Nikon representative.

• Some of the products described in this manual may not be included in the set you have purchased.

• Be sure to read the instruction manual for any other products used in combination with the microscope.

• If you purchased the TE2000-E model, be sure to read the instruction manual supplied with the T-HUBC HUB

controller.

• If you are using the TE2000-U/S together with the T-HUBC HUB controller, be sure to read the instruction manual

supplied with the T-HUBC HUB controller.

Warning / Caution symbols used in this manual

Although Nikon products are designed to provide you with the utmost safety during use, incorrect usage or

disregard of the instructions can cause personal injury or property damage and will lead to the forfeiture of all

claims against warranty. For your safety, read the instruction manual carefully and thoroughly before using the

instrument. Do not discard this manual but keep it near the product for easy reference.

In this manual, safety instructions are indicated with the symbols shown below. Be sure to follow the

instructions indicated with these symbols to ensure correct and safe operation.

Symbol Meaning

Disregarding instructions marked with this symbol may lead to death or serious

injury.

Disregarding instructions marked with this symbol may lead to injury or property

damage.

Meaning of symbols used on the equipment

The symbol appearing on the product indicates the need for caution at all times during use.

Always refer to the instruction manual and read the relevant instructions before manipulating any part to which

the symbol has been affixed.

Symbol Meaning

Caution for heat.

This marking at the top part of the Dia-illuminator and the 12V100W lamphouse calls your

attention on the following;

• Lamphouse becomes very hot during and immediately after the illumination.

• Risk of burns. Do not touch the lamphouse during and immediately after the illumination.

• Make sure that the lamphouse is sufficiently cool before the lamp replacement.

Biohazard

This symbol on the stage calls your attention to the following:

• Spillage of a sample from a vessel onto the microscope presents a biohazard risk.

• To avoid biohazard contamination, do not touch the contaminated portion with your bare

hands.

• Decontaminate the contaminated portion according to the standard procedure of your

laboratory.

1. Intended use of the equipment.

This microscope is intended mainly for use in microscopic observation and in the micr omanipulation of living cells and tissue using diascopic (transmitted) and episcopic (reflected) illumination. It is designed for the main purposes of experimentation and observation, in hospitals or other laboratories, of such cells and tissue within the fields of genetics, immunology, physiology, pharmacology, neurology, cellular biology, and molecular biology.

2. Do not disassemble.

Disassembly may cause malfunction and / or electrical shock, and will lead to the forfeiture of all claims against warranty. experience any problem with the microscope, notify your nearest Nikon representative.

3. Check the Input Voltage.

This microscope uses a power supply for the lamp.

When using the power supply TE-PS30 or TE-PSE30:

Make sure that the input voltage indication on the rear panel matches your regional voltage supply. If the voltages do not match, do not use the microscope; instead, notify your nearest Nikon representative. Using the power supply with the wrong input voltage may cause a short circuit or fire. It may also cause damage to the microscope.

When using the power supply TE2-PS100W:

If you are using the power supply TE2-PS100W, there is no need to check the input voltage since the input voltage of this power supply is AC 100-240V and can be used at any place in the world.

4. Check the AC adapter of the HUB controller (when using the T-HUBC HUB controller).

The HUB controller is powered by the AC adapter. Be sure to use the specified adapter model meeting the requirements given below. Use of any other type of adapter can result in malfunction , excessive heating, and/or fire.

• To prevent malfunction and/or fire, be sure to use the AC adapter in a well-ventilated location. To

ensure that it radiates heat properly and does not overheat, never cover or place any object on the adapter.

• To prevent malfunction, always turn off the power switch (switch to “O”) of the HUB controller before

attaching the AC adapter.

• Specified AC adapter

Manufacturer: PHIHONG ENTERPRISE (Taiwan) Model: PSA30U-120 (N) Rated input voltage: AC 100-240 V, 0.7 A, 50/60 Hz Voltage fluctuation: ±10% Rated output voltage: DC 12 V Rated output current: 2.5 A Others: UL Listed product, GS approved, CE satisfied

Do not disassemble any part other than those described in this manual. If you

5. Power cord for power supply and the power cord for AC adaptor of the HUB controller

To prevent electric shock, always turn off the power switch (switch to “O”) for the power supply and the HUB controller before attaching or detaching the power cord. Use one of the power cords specified below. Use of an improper power cord can result in fire or other hazard. Also note that the power supply is classified as subject to protection class I against electric shock. Therefore, be sure to connect it to a protective ground terminal.

• Using units in areas where the supply voltage is 100 to 120 V

UL Listed detachable power cord set, 3 conductor grounding Type SVT, No. 18 AWG, 3 m long maximum, rated at 125 V AC minimum.

• Using units in areas where the supply voltage is 220 to 240 V

Approved according to EU/EN standards, 3 conductor grounding Type HO5VV-F, 3 m long maximum, rated at 250 V AC minimum.

6. Heat from the light source.

• The lamp and the lamphouse become extremely hot by the lamp illumination. To avoid burns, do not

touch the lamphouse while the lamp is lit or for thirty minutes after it has been turned off.

• To avoid the risk of fire, do not place fabric, paper or highly flammable volatile materials such as

gasoline, petroleum benzine, paint thinner or alcohol near the lamphouse while the lamp is lit or for about thirty minutes after it has been turned off.

• The bottom plate of the power supply becomes hot during use. Do not cover up the ventilation holes

on the side of the power supply.

Continued on the next page

7. Hazardous sample

This microscope is mainly for use in microscopic observation and micromanipulation of living cells and tissue cultures in Petri dishes, microtiter plates, etc. When handling a sample, check to determine whether the sample is hazardous. Handle hazardous samples according to the standard procedure of your laboratory. If the sample is of an infectious nature, wear rubber gloves to avoid infection, and be careful not to spill the sample. In the event of spillage of a sample from a vessel onto the microscope, decontaminate the contaminated portion according to the standard procedure of your laboratory.

1. Check the combination of the lamp, dia-illuminator and power supply.

The dia-illuminator and the power supply must be used in correct combination against the ratings of the lamp and the regional voltage supply. See page 38 to find out the correct combination of these items. Using the equipment in wrong combination will lead to fire, electric shocks or malfunction of the equipment.

2. Turn off the power during assembly, connection/disconnection of the cords, and lamp replacement.

To prevent electric shocks and/or malfunction, always turn off the power switch of the power supply and the T-HUBC HUB controller (flip it to the { side) and unplug the power cord from the wall outlet before assembly, connecting or disconnecting of the cords, and the lamp replace ment.

3. Cautions on lamp replacement.

To avoid burns, wait at least 30 minutes after the lamp is turned off so that the lamp can cool sufficiently. To avoid electric shocks and malfunction, always turn off the power switch (flip it to the { side) and unplug the power cord from the wall outlet before lamp replacement. Securely attach the lamphouse cover to the lamphouse after replacing the lamp. Never light the lamp while the lamphouse cover is open. Do not break used lamps; instead dispose of them as special industrial waste or according to the laws applicable to your municipal waste system.

4. Do not wet the microscope.

If the instrument gets wet, a short circuit may result that may cause malfunction or abnormal heating. If you accidentally spill a liquid on the instrument, immediately turn off the power switch (flip to the { side) and unplug the power cord from the wall outlet. Then use a dry cloth to wipe away the moisture. If any liquid gets inside the instrument, do not use it; instead, notify your nearest Nikon representative.

5. Weak electromagnetic waves.

This microscope emits weak electromagnetic waves. The accuracy of any precision electronic equipment may be adversely affected if positioned too close. If the microscope affects TV or radio reception, move the radio or TV further away from the microscope.

6. Cautions on assembling and installing the microscope.

• Be careful not to pinch your fingers or hands during the assembly.

• The scratches and dirt such as fingerprints on the optical parts (such as lens and filters) will adversely

affect the microscope image. Be careful not to scratch or directly touch the lens and filters.

• This product is a precision optical instrument. Using or storing it under unsuitable conditions may

damage it or may have an adverse effect on its accuracy. See the installation conditions on page 4 and use the product in an adequate environment.

7. Cautions on moving the microscope.

When moving the microscope, do not hold it by the focusing knobs, eyepiece tube, stage, dia-illuminator etc., since these parts can be damaged, or they could come off. Hold the microscope by the bottom front and bottom rear.

8. Be careful of the protruding rack of the T-SR rectangular stage.

The rack of the T-SR rectangular stage will protrude by the stage movement. Be careful not to strike your hands against the rack when reaching the focusing knobs or revolving nosepiece. You may get yourself hurt by the edge of the rack.

Continued on the next page

9. Disposal of the microscope

To avoid biohazard risk, dispose of the microscope as contaminated equipment according to the standard procedure of your laboratory.

Notes on handling the microscope

1. Handle the microscope gently.

This product is a precision optical instrument. Handle it carefully, and do not subject it to strong shocks.

The precision of the objectives in particular can be adversely affected even by weak shocks.

2. Dirt on the lens.

The scratches and dirt such as fingerprints on the optical parts (such as lens and filters) will adversely affect the

microscope image. If these parts get dirty, clean them following the instructions described on “Care and

maintenance” at the end of this manual.

3. Dirt on the lamps.

Do not touch the lamp by bare hands. Dirt or fingerprints on the lamp will cause uneven illumination and shortens

the life of the lamp. When handling lamps, wear gloves.

4. Installation location.

Using or storing the microscope under unsuitable conditions may damage it or may have an adverse effect on its

accuracy. The following conditions should be kept in mind when selecting the installation location.

• Choose a flat surface with little vibration.

• Choose a location less exposed to hazards in the event of collisions, earthquakes, or other potential disasters. If

required to keep the device from falling, use strong rope or other means to secure the microscope to the working

desk or to another heavy, stable item.

• Avoid a brightly lit location such as a room that receives direct sunlight.

• Choose a location that is free from dust or dirt.

• Do not install the microscope in a warm (60°C or more), humid (85% or more) location. (Mold or condensation

will form on the lenses and filters.)

• Leave enough space against the nearby wall since the lamphouse will become hot by lamp illumination.

• When using the “T-DH dia-illuminator 100W”, leave a certain space between the microscope and the nearby wall to

allow the user to look at the caution symbols on the dia-illuminator and the lamphouse. If you are planning to use

the tilting function of the “T-DH dia-illuminator 100W”, even more space is needed for the illuminator to tilt

backward.

• The room light just above the microscope may come into the objective as an extraneous light. (Especially when

using a condenser lens with longer working distance such as SLWD, ELWD and LWD lenses.) To avoid this, we

recommend to turn off the room light above the microscope when observing the image.

5. Focusing knobs.

• Never turn the focus knobs on the left and right sides of the microscope in opposite directions at the same time,

as doing so can result in damage to the microscope.

• Turning the coarse focus knob as far as it will go and then attempting to turn it further will result in damage to the

microscope. Never use undue force to turn the knob.

• (For TE2000-E only) The coarse focus knob turns in sync with motorized Escape/Refocus movements. To prevent

malfunctions, avoid contact with the coarse focus knob during motorized Escape/Refocus movements.

6. Protect the ports.

When not using any of the ports, be sure to attach the supplied cap to it. If not, extraneous light and dusts will enter

the microscope.

Contents

Warning / Caution symbols used in this manual................................................... 1

Meaning of symbols used on the equipment ......................................................... 1

............................................................................................................. 2

! ............................................................................................................. 3

Notes on handling the microscope........................................................................ 4

I. Parts of the microscope and their names .................................................... 6

1. Eyepiece tubes and eyepieces.................................................................... 7

2. Microscope base....................................................................................... 8

3. Dia-illuminators ......................................................................................13

4. Condensers ............................................................................................14

5. Stages, focusing module ..........................................................................15

6. Microscope rear ......................................................................................16

7. Power supplies........................................................................................17

II. Microscopy ................................................................................................ 18

1. Microscope system consisting of TE2000-U, dia-illuminator 100W,

system condenser, and T-TD eyepiece tube D .............................................20

2. Microscope system consisting of TE2000-S, dia-illuminator 30W,

SLWD condenser, and T-TS eyepiece tube S................................................25

3. Microscope system consisting of TE2000-E, dia-illuminator 30W,

SLWD condenser, and T-TS eyepiece tube S................................................29

4. Photomicrography (using a 35-mm camera mounted on the front port) .........34

III. Operation of each part .............................................................................. 37

1. Power ON/OFF ........................................................................................38

2. Brightness adjustment .............................................................................39

3. Optical path switching..............................................................................40

4. Using filters ............................................................................................42

5. Using field diaphragm ..............................................................................42

6. Using condenser aperture diaphragm.........................................................43

7. Eyepiece tube turret ................................................................................44

8. System condenser...................................................................................44

9. Objectives ..............................................................................................45

10. Diopter adjustment .................................................................................47

11. Focusing module .....................................................................................47

12. T-DH dia-illuminator 100W .......................................................................49

13. T-SR rectangular stage ............................................................................50

14. Photomicrography ...................................................................................51

IV. Assembly................................................................................................... 54

V. Troubleshooting ........................................................................................ 61

VI. Care and maintenance............................................................................... 64

VII. Technical specifications............................................................................. 65

System diagram ........................................................................................ 70

I. Parts of the microscope and their names

Parts of the microscope can be selected according to your purpose.

Exceptionally, the combination of the lamp, dia-illuminator and the power supply is fixed. Never use these

parts in combination other than specified.

(Refer to page 38 for the correct combination of the lamp, dia-illuminator and the power supply.)

The T-HUBC HUB controller, which mounts on the rear side of the microscope, allows the user to control

attached motorized units. For details, refer to the instruction manual provided with the T-HUBC HUB

controller.

Dia-illuminator

P.13

Condenser P.14

Eyepiece tube

P. 7

Stage P.15

Microscope rear P.16

Power supply

P.17

Microscope base P. 8

This is a photograph of TE2000-U microscope with T-DH dia-illuminator 100W, LHS-H100P-1 12V100W

lamphouse, 12V100W halogen lamp, TE2-PS100W power supply, T-SR rectangular stage, T-TD

binocular eyepiece tube D, CFI 10X eyepieces, T-N6 sextuple nosepiece, system condenser, objectives,

power cord, etc.

I. Parts of the microscope and their names

1. Eyepiece tubes and eyepieces

The following eyepiece tubes can be mounted on the observation port of the microscope.

T-TD eyepiece tube D

A: Diopter adjustment ring

B: Eyepiece

C: Eyepiece tube turret

T-TS eyepiece tube S

O: Open B: Bertrand lens (with focusing screw) C: Close (Shutter) M: 2.5X magnifier

A: Diopter adjustment ring

B: Eyepiece

T-TERG ergonomic eyepiece tube

A: Diopter adjustment ring

B: Eyepiece

C: Eyepiece tube turret

O: Open B: Bertrand lens (with focusing screw) C: Close (Shutter)

T-TI intermediate tube

A: Clamp screw for holding various devices

B: Mount for various devices

such as Trinocular eyepice tube for upright microscopes Teaching head

I. Parts of the microscope and their names

2. Microscope base

Note: Be sure to cover unused ports with supplied caps to prevent the entry of external light and dust.

TE2000-E, right side

Eyepiece-tube clamp screw

Observation port

Install the eyepiece tube.

Front port

Photo-mask dial

Clockwise turn: OUT Counterclockwise turn: IN

Intermediate magnification dial

1X: No intermediate magnification

1.5X: 1.5X intermediate magnification for all ports

REFOCUS switch

Raises the objective to the memorized position.

ESCAPE switch

Lowers the objective to the Escape position.

Fine focus knob

The distance of travel achieved by one rotation of the knob can be set to Fine, Middle, or Coarse. The knob is set to Coarse at the factory.

T-RCP remote control pad

Not required when the microscope is controlled using a PC

REFOCUS

COARSE FOCUS

ESCAPE

Objective

Nosepiece

Mount for analyzer

Install the analyzer here for DIC microscopy.

Adapter clamp screw

Left-side port

Optical path switch-over section holding screw

This screw is installed for transportation. Be sure to remove the screw before using the microscope.

TE-2000E, left side

T-HUBC HUB controller

The specified AC adapter is required.

Adapter clamp screw

Right-side port

Bottom port

DIA-LAMP ADJ key

Used to adjust the view field brightness. This key can be used only when the T-DH dia-illuminator 100W is installed.

Turn on the green indicators.

Light path key

Changes the optical path.

Observation 20%

Front 80%

Observation 100%

Left 100%

Bottom 100%

Stage

Black cover for elevating section

Remove the cover when installing the epi-fl attachment.

Mount for cassette holder

For the installation of the cassette holder for epi-fl microscopy

Dia-illumination ON/OFF switch

Brightness adjustment dial

This dial is disabled when the remote control pad is used for bright adjustment.

Observation 20%

Right 80%

Coarse focus knob

I. Parts of the microscope and their names

Note: Be sure to cover unused ports with supplied caps to prevent the entry of external light and dust.

TE2000-U, right side

Eyepiece-tube clamp screw

Observation port

Install the eyepiece tube.

Adapter clamp screw

Front port

Photo-mask dial

Clockwise turn: OUT Counterclockwise turn: IN

Intermediate magnification dial

1X: No intermediate magnification

1.5X: 1.5X intermediate magnification for all ports

Optical path switch-over dial

1: Observation 100% 2: Observation 20%, Right 80% 3: Not used (Bottom port 100%) 4: Observation 20%, Front 80% 5: Left 100%

Objective

Nosepiece

Mount for analyzer

Install the analyzer here for DIC microscopy.

Adapter clamp screw

TE2000-U, left side

Right-side port

Fine focus knob

Coarse focus knob

Objective refocusing ring

Forward turn: Release Backward turn: Lock

Stage

Black cover for elevating section

Remove the cover when installing the epi-fl attachment.

Mount for cassette holder

For the installation of the cassette holder for epi-fl microscopy

Dia-illumination ON/OFF switch

Left-side port

Coarse focus torque adjustment ring

Forward turn: Decreases torque Backward turn: Increases torque

Brightness adjustment dial

This dial is disabled when the remote control pad is used for bright adjustment.

Fine focus knob

Coarse focus knob

The bottom port changeover lever is provided on the left-hand side of the TE2000-U bottom port type.

See P. 41 for optical path switching.

I. Parts of the microscope and their names

Note: Be sure to cover unused ports with provided caps to prevent the entry of external light and dust.

Eyepiece-tube clamp screw

Observation port

Install the eyepiece tube.

Optical path switch-over dial

EYE: Observation 100% SIDE: Observation 20%, Left 80%

Objective

Nosepiece

Mount for analyzer

Install the analyzer here for DIC microscopy.

Adapter clamp screw

TE2000-S, right side

TE2000-S, left side

Fine focus knob

Coarse focus knob

Stage

Black cover for elevating section

Remove the cover when installing the epi-fl attachment.

Mount for cassette holder

For the installation of the cassette holder for epi-fl microscopy

Dia-illumination ON/OFF switch

Left-side port

Coarse focus torque adjustment ring

Forward turn: Decreases torque Backward turn: Increases torque

Brightness adjustment dial

This dial is disabled when the remote control pad is used for bright adjustment.

Fine focus knob

Coarse focus knob

I. Parts of the microscope and their names

Various adapters for the front port (TE2000-S is not equipped with front port.)

T-BFA F-mount adapter

Digital still camera with F-mount such as D1 can be mounted.

T-BSLR SLR camera adapter

Single-lens reflex camera such as FE10, F70, F90 and F5 can be mounted.

T-BDCA direct C-mount adapter

Digital still camera for the microscopes such as DXM1200, can be mounted here.

Various adapters for side port

Side port adapter

This adapter is supplied with the microscope.

T-BPA photo adapter

Double port adapter

Various TV adapters

Various adapters for bottom port

(TE2000-U, except for the bottom-port type, and TE2000-S are not equipped with bottom port)

Bottom port adapter

This adapter is supplied with the microscope.

Various TV adapters

I. Parts of the microscope and their names

T-EFN Focus Knob

(supplied with the TE2000-E only. The knob is incompatible with the TE2000-U and TE2000-S.)

The TE2000-E provides focus adjustment in various positions with the supplied T-EFN focus

knob. You can make fine focus adjustments by connecting the T-EFN focus knob to the T-

HUBC HUB controller AUX connector and by switching the knob selector switch on. (Switching

the selector switch off permits fine focus adjustments using the fine movement knob

provided on the right side of the TE2000-E.)

The T-EFN focus knob is comprised of the focus knob main unit and the base plate, which are

connected by a magnet.

The direction of the T-EFN focus knob can be switched between right and left by changing the

main unit's position relative to the base plate. Note that the focus adjustment knob can be

operated independently with the knob facing up.

Knob rightward rotation (clockwise): Raises the objective (brings the objective

closer to the specimen).

Knob leftward rotation (counterclockwise): Lowers the objective (moves the objective

farther from the specimen).

Fine movement

Selector switch

Knob facing left Knob facing right Knob facing up

Caution: The T-EFN focus knob main unit and the base plate are connected by a magnet. Handle carefully.

Lifting them while they are connected may cause the base plate to detach and fall.

I. Parts of the microscope and their names

3. Dia-illuminators

Dia-illuminators should be used in specified pairs with the lamp (12V100W or 6V30W). A lamphouse is

needed for the T-DH dia-illuminator 100W.

T-DH dia-illuminator 100W and LHS-H100P-1 12V100W lamphouse

A: Field aperture diaphragm lever

B: Filter sliders

C: Condenser refocusing clamp

(Works only when LWD condenser lens is attached.)

D: Condenser focus knob

E: Condenser clamp screw

F: Condenser mount positioning pin

G: Condenser mount positioning groove

H: Condenser mount rotation clamp

screw

I: Condenser centering screw

J: Condenser mount (rotatable)

K: LHS-H100P-1 12V100W lamphouse

L: Lamphouse clamp screw

M: Lamphouse cover clamp screw

N: Condenser holder (removable)

O: Condensr holder clamp screw

P: Lamp cable

Q: Condenser holder fall-stop screw

T-DS dia-illuminator 30W

A: Dustproof slider (removable)

B: F stop slider (removable)

C: Condenser mount

D: 6V30W lamp housing

E: Filter slider

F: M4 screw holes for additional device mounting

I. Parts of the microscope and their names

4. Condensers

System condenser

When mounted on When mounted on T-DS dia-illuminator 30W

T-DH dia-illuminator 100W (Only for HMC observation.)

A: Condenser aperture diaphragm

B: Condenser module

C: Condenser module clamp screw

D: Condenser lens

(3 types available; ELWD, LWD, HMC)

E: Annular diaphragm centering screw

(on Ph modules only)

ELWD-S condenser SLWD condenser

A： Centering handle clamp screw

B： Centering handle

C： Turret

SLWD condenser can only be mounted on T-DS dia-illuminator 30W.

5. Stages, focusing module

T-SR rectangular stage

A: Stage ring

B: Y-axis stage movement knob

C: X-axis stage movement knob

T-SP plain stage

A: Stage ring

I. Parts of the microscope and their names

6. Microscope rear

Mount for epi-fl

attachment

Mount for T-HUBC

HUB controller

LAMP CTRL

connector Connect

the power supply

or HUB controller.

MIC CTRL1 connector

Connect the HUB controller. Not provided on TE2000-U or TE2000-S

MIC CTRL2 connector

Connect the HUB controller. Not provided on TE2000-U or TE2000-S

(The HUB controller's connectors are attached with various motorized units.)

Epi-fl attachment

NOSEPIECE

Connect the T-ND6-E

sextuple motorized

DIC nosepiece.

ANALYZER

Connect the T-A-E

motorized DIC analyzer

Connect the T-FLEW-E

EX FILTER

EX filter wheel.

FL SHUTER

Connect the T-FL-E

motorized epi-fl

attachment.

REMOTE

Connect the T-RCP

remote control pad.

and epi-fl attachment installed to the rear side of the microscope.

The photo shows the T-HUBC HUB controller

TE-PS

Connect the power supply.

T-HUBC HUB controller

This device controls the attached motorized units. It must be installed when the TE2000-E is used. Installation of this controller is optional on the TE2000-U and TE2000-S.

CONDENSER

Connect the T-CT-E motorized condenser turret.

FL BLOCK

Connect the T-FLC-E motorized cassette holder.

BA FILTER

Connect the T-FLBW-E BA filter wheel.

AUX

Connect the T-EFN focus knob here. (The T-EFN focus knob is supplied with the TE2000-E only.)

SHUTTER

Refer to "VI-21 Connection of external equipment" in the instruction manual supplied with the HUB controller.

STAGE

Refer to "VI-21 Connection of external equipment" in the instruction manual supplied with the HUB controller.

Connect a PC.

EXP

Refer to "VI-22 Connection to the EXP connector" in the instruction manual supplied with the HUB controller.

7. Power supplies

The bottom of the power supply becomes hot while it is in use. Do not obstruct the

air vents on the sides of the power supply.

TE2-PS100W power supply

Pilot lamp

Turns on when the power is on.

I. Parts of the microscope and their names

EXTERNAL switch

Turning the switch ON activates the brightness adjustment dial on the microscope or the DIA-LAMP adjustment key on the T-RCP remote control pad (when the T-DH dia-illuminator 100W is used).

Turning this switch OFF activates the brightness adjustment dial on the power supply.

OUTPUT connector

This is the lamp output connector. Connect the lamp cable from the dia-illuminator to this connector.

TE-PS30 power supply (for 100−−−−120 V area) TE-PSE30 power supply (for 220−−−−240 V area)

Pin 2 : ditto

CTRL connector

Connect the LAMP CTRL connector on the rear of the microscope using a control cable. This device canno be used to connect the T-HUBC HUB controller.

Pin 3 : Lamp ON/OFF: Input (Open: ON, 0 V: OFF) Pin 4 : 0 V

II. Microscopy

When using the system consisting of the TE2000-E and dia-illuminator 100W

For the microscopy procedure, refer to the instruction manual supplied

with the T-HUBC HUB controller.

The T-HUBC HUB controller, which mounts on the rear of the microscope, controls the operations of all

attached motorized units. Note that when the T-HUBC HUB controller is mounted on the rear of the

microscope, the microscope cannot be operated in the same way as when manual operation is used. Be

sure to read the instruction manuals supplied with the T-HUBC HUB controller and T-RCP remote control

pad to ensure proper preparation and operation of the microscope.

Note that the following two operations cannot be controlled using the T-HUBC HUB controller.

• 6V30W lamp ON/OFF: Use the dia-illumination ON/OFF switch on the microscope main body.

• 6V30W lamp voltage adjustment: Use the brightness adjustment dial on the microscope main body or

power supply.

When using the TE2000-U or TE2000-S

with the T-HUBC HUB controller mounted on the rear

For the microscopy procedure, refer to the instruction manual supplied

with the T-HUBC HUB controller.

The T-HUBC HUB controller, which is mounted on the rear of the microscope, controls the operations of all

attached motorized units. Note that when the T-HUBC HUB controller is mounted on the rear of the

microscope, the microscope cannot be operated in the same way as when manual operation is used. Be

sure to read the instruction manuals supplied with the T-HUBC HUB controller and T-RCP remote control

pad to ensure proper preparation and operation of the microscope.

Note that the following five operations cannot be controlled using the T-HUBC HUB controller.

• Optical path switchover: Use the optical path switchover dial on the microscope main body.

• Vertical focus motion control from PC: Use the fine/coarse focus knob on the microscope main body.

• Z-axis display: The Z-axis position data cannot be obtained.

• 6V30W lamp ON/OFF: Use the dia-illumination ON/OFF switch on the microscope main body.

• 6V30W lamp voltage adjustment: Use the brightness adjustment dial on the microscope main body or

power supply.

II. Microscopy

The TE2000 series is the system microscopes that can be flexibly configured to meet specific applications. There are three

main body types, two types of dia-illuminators, four types of eyepiece tubes, and various other parts variations. The

following section describes the basic microscopy procedure based on three standard system configurations.

• Refer to "III. Operation of each part" for details on each operation.

• If your microscope is configured differently from the system described in the manual, refer to the applicable

explanations in "III. Operation of each part."

• If your microscope is not pre-assembled, see "IV. Assembly" first.

• If your microscope is mounted with the epi-fl, DIC, or other attachments, refer to the instruction manuals supplied with

the respective attachments.

1. Microscope system consisting of TE2000-U, dia-illuminator 100W, system condenser, and T-TD eyepiece

tube D

1-1. Bright-field microscopy

1-2. Phase-contrast microscopy

2. Microscope system consisting of TE2000-S, dia-illuminator 30W, SLWD condenser, and T-TS eyepiece

tube S

2-1. Bright-field microscopy

2-2. Phase-contrast microscopy

3. Microscope system consisting of TE2000-E, dia-illuminator 30W, SLWD condenser, and T-TS eyepiece

tube S

3-1. Bright-field microscopy

3-2. Phase-contrast microscopy

4. Photomicrography (using a 35-mm camera mounted on the front port)

Microscope system consisting of TE2000-U, dia-illuminator 100W, system condenser, and T-TD eyepiece

tube D

 Supplementary information

When conducting bright-field or phase-contrast microscopy with a microscope system consisting of the TE2000-E,

dia-illuminator 100W, system condenser, and T-TD eyepiece tube D, refer to the instruction manual supplied with the

T-HUBC HUB controller since it provides detailed information.

Before using the instrument, read the "WARNING," "CAUTION," and "Notes on handling the microscope" sections at

the beginning of this manual and be sure to follow the instructions therein.

Also refer to the instruction manuals supplied with other products (such as the epi-fl and DIC attachments) used in

combination with the microscope, and follow the instructions written.

II. Microscopy

”

1. Microscope system consisting of TE2000-U, dia-illuminator 100W, system condenser, and T-TD eyepiece tube D

The following instructions are based on a microscope equipped with the parts listed below.

TE2000-U microscope

T-TD eyepiece tube D

CFI 10X eyepiece

T-DH dia-illuminator 100W

LHS-H100P-1 12V100W lamphouse

12V100W halogen lamp

TE2-PS100W power supply

System condenser

LWD condenser lens

Condenser module for bright-field microscopy

Condenser module for phase-contrast microscopy

T-SR rectangular stage

T-N6 sextuple nosepiece

Objectives for bright-field microscopy

Objectives for phase-contrast microscopy

Others

1-1. Bright-field (BF) microscopy

Key point: Detach all optical elements required for other types of observation from the optical path. Position the

condenser correctly (adjust the focus and centering), and adjust the clarity of the image by moving the

“aperture diaphragm”.

1. Reset the convenient functions.

1) Turn the “condenser refocusing clamp” on the dia-

illuminator counterclockwise to release.

2) Set the “eyepiece tube turret” to position < O >.

3) Set the “intermediate magnification dial” on the right side

of the microscope to position < 1X >.

4) Turn the “objective refocusing ring

focus knob on the right side of the microscope

counterclockwise to release.

behind the fine/coarse

2. Turn on the dia-illumination.

”

1) Set the “EXTERNAL switch” on the rear side of the power

supply to ON.

2) Turn on the “power switch” of the power supply. (Flip the

switch to .)

3) Press the “dia-illumination ON/OFF switch

of the microscope to turn on the lamp.

3. Adjust the lamp to ensure color fidelity.

1) Set the “brightness adjustment dial

microscope to the < 12V100W > indication.

2) Move the “NCB11 filter” on the dia-illuminator into the

optical path.

3) Move the “ND4 filter

path.

on the dia-illuminator into the optical

on the left side

on the left side of the

II. Microscopy

4. Adjust the optical path.

1) Move the < 10X > objective into the optical path.

2) Set the “optical path switchover dial

microscope to position < 1 >. (-> Direct 100% light to the

observation port.)

3) Raise the “field diaphragm lever” on the dia-illuminator

completely to open the field diaphragm fully.

4) Turn the “aperture diaphragm lever

to the right limit to open the “apertur e d iaphragm” fully.

5) Turn the condenser focus knob to lower the condenser

mount to the lowest position.

 Supplementary informat ion

When the system condenser is mounted with the “ELWD

condenser lens”, position the condenser mount at the

location about 1 cm below the uppermost position.

When using the “ELWD-S condenser”, position the

condenser mount at the location about 2 cm below the

uppermost position.

5. Set the microscope for bright-field microscopy.

1) Set the “condenser turret” to position < A >.

6. Adjust the diopter and interpupillary distance.

1) Turn the “photo-mask dial” on the front side of the

microscope counterclockwise to move the photo-mask into

the optical path.

2) Look into the left eyepiece with your left eye. Turn the

“diopter adjustment ring” of the left eyepiece to bring the

double crosshairs of the photo-mask into focus.

3) Look into the right eyepiece with your right eye. Turn the

“diopter adjustment ring” of the right eyepiece to bring the

double crosshairs of the photo-mask into focus.

on the right side of the

on the system condense

Turn the diopter adjustment ring

so that the double crosshairs come into focus.

Adjust the interpupillary distance

to consolidate the view fields of both eyepieces.

II. Microscopy

Field of view

Field diaphragm image

Field of view

Field diaphragm image

”

4) Turn the “photo-mask dial” on the front side of the

microscope clockwise to remove the photo-mask from the

optical path.

5) Adjust the distance between the eyepieces to consolidate

the view fields of both eyepieces.

7. Adjust the focus.

1) Place a specimen on the stage.

2) While looking into the eyepieces, turn the “fine/coarse

focus knob” to bring the specimen into focus.

8. Center the condenser.

1) Make sure that the < 10X > objective is in the optical path.

2) Lower the “fie ld dia phragm lever” on the dia-illuminator

until the field diaphragm image appears in the field of view.

3) Turn the “condenser focus knob” to bring the field

diaphragm image into focus.

4) Turn the two “condenser centering screws” to move the

field diaphragm image to the center of the view field.

5) Select the < 40X > objective.

6) Adjust the posit ion of the “fie ld diaphragm lever” on the

dia-illuminator until the size of the field diaphragm image is

about the same as that of the view field.

7) Turn the two “condenser centering screws” on the dia-

illuminator to move the field diaphragm image to the center

of the view field.

9. Conduct observation.

1) Select an objective of desired magnification.

2) Move the “aperture diaphragm lever” on the system

condenser to set the opening to 70 to 80% of the N.A.

(numerical aperture) of the objective. (-> Set the

“eyepiece tube turret

lens focusing screw to perform focusing, and adjust the size

of the image while viewing the exit pupil of objective and

aperture diaphragm image.)

3) Move the “field diaphragm lever” on the dia-illuminator

until the size of the field diaphragm image is about the

same as that of the view field.

4) Move the “ND filter

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, the brightness

can be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

to position < B >, turn the Bertrand

on the dia-illuminator in and out of the

II. Microscopy

”

10. Change the specimen.

Use the “objective refocusing ring” on the microscope main

body, the “tilting” of the dia-illuminator, and the

“condenser refocusing clamp” to facilitate specimen

replacement.

11. After the completion of microscopic procedures

1) Turn off the “power switch” of the power supply. (Flip the

switch to {.)

2) After the lamphouse section cools, place the vinyl cover on

the microscope.

1-2. Phase-contrast (Ph) microscopy

Key point: Move Ph objective and condenser module with the same Ph code into the optical path, and align (center) the

phase plate in the objective and the annular diaphragm in the condenser module before conducting

microscopy.

1. Focus on the specimen using bright-field (BF) microscopy.

2. Set the microscope for phase-contrast microscopy.

1) Move a Ph objective into the opt ical path.

2) Turn the “condenser turret

as that of the objective placed in the optical path in step 1).

3) Turn the “aperture diaphragm lever” on the condenser to

the right limit to open the “aperture diaphragm” fully.

 Supplementary informat ion

If the “aperture diaphragm

path of the annular diaphragm will be restricted,

preventing the realization of the appropriate phase

contrast effect.

4) Raise the “field diaphragm lever” on the dia-illuminator to

the limit to open the field diaphragm fully.

 Supplementary informat ion

When “ELWD-S condenser” is used:

The “aperture diaphragm” of the “ELWD-S condenser”

works only with the bright-field optica l path, and it does

not affect the phase-contrast optical path. Therefore,

there is no need to open the “aperture diaphragm” fully

in step 3).

All the annular diaphragms of the “ELWD-S condenser

will be simultaneously centered when the PhL

diaphragm is centered. Therefore, move a PhL

objective in the optical path, set the “condenser turret

to the "PhL" indication, and proceed to the next step.

and set it to the same Ph code

is not fully open, the optical

II. Microscopy

”

3. Center the annular diaphragm.

1) Set the “eyepiece tube turret” to position < B >, and turn

the Bertrand lens focusing screw to bring the annular

diaphragm image into focus.

2) Using a hexagon screwdriver, turn the two “annular

diaphragm centering screws” on the condenser module so

that the annular diaphragm image coincides with the phase

plate image in the objective.

3) Return the eyepiece tube turret to position < 0 >.

4. Conduct observation.

1) Fully open the “aperture diaphragm” of the condenser.

2) Move the “field diaphragm lever

that the size of the field diaphragm image is about the same

as that of the view field.

3) Remove the “NCB11 filter” on the dia-illuminator from the

optical path, and move the “GIF filter

(-> To improve the contrast)

4) Move the “ND filter” on the dia-illuminator in and out of the

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, the brightness

can be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

on the dia-illuminator so

into the optical path.

5. Conduct observations using an objective with a different magnification value.

1) Move a different Ph objective of desired magnification into

the optical path.

2) Turn the “condenser turret

as that of the objective placed in the optical path in step 1).

3) Center the annular diaphragm placed in the optical path.

(-> See the procedure 3.)

 Supplementary informat ion

When the system condenser is installed with the Ph

condenser module, it is necessary to center all Ph

modules.

When the “ELWD-S condenser” is used, centering the

PhL diaphragm results in the centering of all annular

diaphragms.

6. Change the specimen.

Use the “objective refocusing ring

body, the “tilting” of the dia-illuminator, and the

“condenser refocusing clamp” to facilitate specimen

replacement.

7. Upon the completion of microscopy

Follow the same procedure as for bright-field (BF)

microscopy.

and set it to the same Ph code

on the microscope main

2. Microscope system consisting of TE2000-S,

”

dia-illuminator 30W, SLWD condenser, and T-TS eyepiece tube S

The following instructions are based on a microscope equipped with the parts listed below.

TE2000-S microscope

T-TS eyepiece tube S

CFI 10X eyepiece

T-DS dia-illuminator 30W

6V30W halogen lamp

TE-PS30 power supply

SLWD condenser

T-SR rectangular stage

T-N6 sextuple nosepiece

Objectives for bright-field microscopy

Objectives for phase-contrast microscopy

Others

II. Microscopy

2-1. Bright-field (BF) microscopy

Key point: Detach all optical elements required for other types of observation from the optical path. The size of the field

diaphragm of the SLWD condenser is fixed. There is no “aperture diaphragm”.

1. Turn on the dia-illumination.

1) Make sure that the input voltage indication on the rear side

of the power supply corresponds with the voltage of the

commercial power source in the area. (-> If different, do

not turn on the switch. Contact your nearest Nikon

representative immediately.)

2) Turn on the “CTRL switch” on the rear side of the power

supply.

3) Turn on the “power switch” of the power supply. (Flip the

switch to .)

4) Press the “dia-illumination ON/OFF switch

of the microscope to turn on the lamp.

2. Adjust the lamp to ensure color fidelity.

1) Set the “brightness adjustment dial” on the left side of the

microscope to the < 6V30W > indication.

2) Move the “NCB11 filter” on the dia-illuminator into the

optical path.

3) Move the “ND4 filter” on the dia-illuminator into the optical

path.

on the left side

II. Microscopy

”

3. Adjust the optical path.

1) Move the < 10X > objective into the optical path.

2) Set the “optical path switchover dial

microscope to position < EYE >. (-> Direct 100% of the

light to the observation port.)

3) Place a specimen on the stage.

4. Set the microscope for bright-field microscopy.

1) Slide the Ph annular diaphragm slider to move the < Open

> position into the optical path.

2) While looking into the eyepieces, turn the fine/coarse focus

knob to bring the specimen into focus.

5. Adjust the diopter and interpupillary distance.

1) Turn the “diopter adjustment rings” of both right and left

eyepieces so that the engraved line of each diopter

adjustment ring aligns with the outer ring rim of the

eyepiece. (This sets the diopter adjustment to the "0"

position.)

2) Move the < 40X > objective into the optical path.

3) While looking into the left eyepiece with your left eye, turn

the “fine/coarse focus knob

specimen into focus.

4) Move the < 10X > objective into the optical path.

5) While looking into the left eyepiece with your left eye, turn

the “diopter adjustment ring

the specimen into focus. (Do not touch the fine/coarse

focus knob on the microscope main body in this step.)

6) Repeat steps 2) through 5) two more times.

7) Adjust the right side by following the same procedure.

(Substitute "right" for "left" in steps 2) through 5), and

repeat these steps three times.)

8) Adjust the interpupillary distance to consolidate the view

fields of both eyepieces.

on the right side of the

of the microscope to bring the

of the left eyepiece to bring

4-5)

4-8)

4-1)

Engraved line

Diopter adjustment ring

Outer ring rim

4-2) 4-4) 4-3)

3-2)

6. Conduct observation.

1) Select an objective of desired magnification.

2) Move the “ND filter

on the dia-illuminator in and out of the

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, the brightness

can be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

7. Upon the completion of microscopy

1) Turn off the power switch of the power supply. (Flip the

switch to {.)

2) After the lamp mounting section cools, place the vinyl cover

on the microscope.

II. Microscopy

”

2-2. Phase-contrast (Ph) microscopy

Key point: Slide the Ph annular diaphragm slider to move the annular diaphragm with the same Ph code as the objective

into the optical path, then conduct microscopy. Note that the PhL diaphragm must be centered.

1. Focus on the specimen using bright-field microscopy.

2. Set the microscope for phase-contrast microscopy.

1) Move a Ph objective into the opt ical path.

2) Slide the Ph annular diaphragm slider to move the annular

diaphragm < with the same Ph code > as the objective

placed in the optical path in step 1) into the optical path.

3. Center the PhL diaphragm.

1) Move the < PhL > objective into the optical path.

2) Slide the Ph annular diaphragm slider to move the < PhL >

diaphragm into the optical path.

3) Remove one eyepiece, and insert the centering telescope

using the adapter.

4) Turn the eyepiece of the “centering telescope

annular diaphragm image into focus.

5) Turn the two “annular diaphragm centering screws” on the

condenser so that the annular diaphragm image coincides

with the phase plate image in the objective.

6) Reinsta ll the eyepiece to the origina l position.

to bring the

4. Conduct observation.

1) Remove the “NCB11 filter” on the dia-illuminator from the

optical path, and move the “GIF filter” into the optical path.

(-> To improve the contrast)

2) Move the “ND filter

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, the brightness

can be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

on the dia-illuminator in and out of the

II. Microscopy

5. Conduct observations using an objective with a different magnification value.

1) Move a different Ph objective of desired magnification into

the optical path.

2) Slide the Ph annular diaphragm slider to set to the same Ph

code as that of the objective placed in the optical path in

step 1).

6. Upon the completion of microscopy

Follow the same procedure as for bright-field (BF)

microscopy.

II. Microscopy

”

3. Microscope system consisting of TE2000-E, dia-illuminator

30W, SLWD condenser, and T-TS eyepiece tube S

The following instructions are based on a microscope equipped with the parts listed below.

TE2000-E microscope

T-HUBC HUB controller

AC adapter

Power cord for AC adapter

T-RCP remote control pad

T-TS eyepiece tube S

CFI 10X eyepiece

T-DS dia-illuminator 30W

6V30W halogen lamp

TE-PS30 power supply

SLWD condenser

T-SR rectangular stage

T-N6 sextuple nosepiece

Objectives for bright-field microscopy

Objectives for phase-contrast microscopy

Others

3-1. Bright-field (BF) microscopy

Key point: Detach all optical elements required for other types of observation from the optical path. The size of the field

diaphragm of the SLWD condenser is fixed. There is no aperture diaphragm. Since the 6V30W lamp ON/OFF

and lamp voltage adjustment cannot be controlled using the T-RCP remote control pad, use the switch and

control on the microscope main body.

1. Turn on the dia-illumination.

1) Make sure that the input voltage indication on the rear side

of the power supply corresponds with the voltage of the

commercial power source in the area. (-> If different, do

not turn on the switch. Contact your nearest Nikon

representative immediately.)

2) Turn on the “CTRL switch” on the rear side of the power

supply.

3) Turn on the “power switch” of the power supply. (Flip the

switch to .)

4) Turn on the “power switch

switch to .)

5) Press the dia-illumination ON/OFF switch on the left side of

the microscope to turn on the lamp.

of the HUB controller. (Flip the

II. Microscopy

”

2. Adjust the lamp to ensure color fidelity.

1) Set the “brightness adjustment dial” on the left side of the

microscope to the < 6V30W > indication.

2) Move the “NCB11 filter

path.

3) Move the “ND4 filter” on the dia-illuminator into the optical

path.

3. Adjust the optical path.

1) Press the “OBJECTIVE

the < 10X > objective into the optical path.

2) Press the “LIGHT PATH

100% light toward the observation port.

3) Place a specimen on the stage.

4. Set the microscope for bright-field microscopy.

1) Slide the Ph annular diaphragm slider to move the < Open >

position into the optical path.

2) While looking into the eyepieces, turn the “fine/coarse focus

knob” to bring the specimen into focus.

on the dia-illuminator into the optical

key on the remote control pad to move

key on the remote control pad to point

5. Adjust the diopter and distance between the eyepieces.

1) Turn the “photo-mask dial” on the front side of the microscope

counterclockwise to move the photo-mask into the optical

path.

2) Look into the left eyepiece with your left eye. Turn the

“diopter adjustment ring” of the left eyepiece to bring the

double crosshairs of the photo-mask into focus.

3) Look into the right eyepiece with your right eye. Turn the

“diopter adjustment ring” of the right eyepiece to bring the

double crosshairs of the photo-mask into focus.

4) Turn the “photo-mask dial” on the front side of the microscope

clockwise to remove the photo-mask from the optical path.

5) Adjust the interpupillary distance to consolidate the view fields

of both eyepieces.

Turn the diopter adjustment ring

so that the double crosshairs come into focus.

Adjust the interpupillary distance

to consolidate the view fields of both eyepieces.

6. Conduct observation.

1) Press the “OBJECTIVE” key on the remote control pad to

select the desired magnification.

2) Move the ND filter on the dia-illuminator in and out of the

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, brightness can

be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

7. Upon the completion of microscopy

1) Turn off the power switch of the HUB controller. (Flip the

switch to {.)

2) Turn off the power switch of the power supply. (Flip the

switch to {.)

3) After the lamp mounting section cools, place the vinyl cover

on the microscope.

II. Microscopy

”

3-2. Phase-contrast (Ph) microscopy

Key point: Slide the Ph annular diaphragm slider to move the annular diaphragm with the same Ph code as the objective

into the optical path, then conduct microscopy. Note that the PhL diaphragm must be centered.

1. Focus on the specimen using bright-field microscopy.

2. Set the microscope for phase-contrast microscopy.

1) Press the “OBJECTIVE” key on the remote control pad to

move a Ph objective into the optical path.

2) Slide the Ph annular diaphragm slider to move the annular

diaphragm < with the same Ph code > as the objective

placed in the optical path in step 1) into the optical path.

3. Center the PhL diaphragm.

1) Press the “OBJECTIVE” key on the remote control pad to

move a PhL objective into the optical path.

2) Slide the Ph annular diaphragm slider to move the < PhL >

diaphragm into the optical path.

3) Remove one eyepiece, and insert the “centering telescope

using the adapter.

4) Turn the eyepiece of the “centering telescope” to bring the

annular diaphragm image into focus.

5) Turn the two “annular diaphragm centering screws

condenser so that the annular diaphragm image coincides

with the phase plate image in the objective.

6) Return the eyepiece to the original position.

on the

4. Conduct observation.

1) Remove the “NCB11 filter

optical path, and move the “GIF filter” into the optical path.

(-> To improve the contrast)

2) Move the “ND filter” on the dia-illuminator in and out of the

optical path to adjust the brightness of the view field.

 Supplementary informat ion

If precise color fidelity is not required, the brightness

can be adjusted by varying the lamp voltage using the

“brightness adjustment dial” on the left side of the

microscope.

on the dia-illuminator from the

5. Conduct observations using an objective with a different

magnification value.

1) Press the “OBJECTIVE” key on the remote control pad to

move a different Ph objective of desired magnification into

the optical path.

2) Slide the Ph annular diaphragm slider to set to < the same

Ph code > as that of the objective placed in the optical path

in step 1).

6. Upon the completion of microscopy

Follow the same procedure as for bright-field (BF) microscopy.

II. Microscopy

4. Photomicrography (using a 35-mm camera mounted on the

front port)

The following describes the procedure for taking pictures with a daylight-type color film using a single-lens reflex camera

attached to the front port of the microscope. Regarding the operation of the camera, refer to the instruction manual

supplied with the camera. Load the camera with film before starting the following steps. Also refer to "14.

Photomicrography" in "III. Operation of each part."

The following instructions are based on a microscope equipped with the parts listed below.

TE2000-U microscope

T-TD eyepiece tube D

CFI 10X eyepiece

T-DH dia-illuminator 100W

LHS-H100P-1 12V100W lamphouse

12V100W halogen lamp

TE2-PS100W power supply

System condenser

LWD condenser lens

Condenser module for bright-field microscopy

Condenser module for phase-contrast microscopy

T-SR rectangular stage

T-N6 sextuple nosepiece

Objectives for bright-field microscopy

Objectives for phase-contrast microscopy

Others

1. Focus on the specimen using bright-field (BF) microscopy.

2. Set up the 35-mm camera.

1) Mount the 35-mm camera on the front port.

2) Set the “exposure mode” of the camera to < Aperture

Priority Auto Mode >.

3) Set the “exposure compensation” of the camera to < +2/3

>. (-> Slight overexposure produces better results when

using a 35-mm film camera mounted on the front port.)

4) Adjust the other settings (ISO value, photometry mode,

etc.) on the camera.

3. Adjust the lamp for color fidelity.

”

1) Set the “brightness adjustment dial” on the left side of the

microscope to the < 12V100W > indication.

2) Move the “NCB11 filter” on the dia-illuminator into the

optical path.

3) Move the “ND4 filter

path.

 Supplementary informat ion

If precise color fidelity is not required, such as when

monochromic film is used, the “brightness adjustment

dial” on the left side of the microscope can be set to any

position.

In the case of the “

the brightness adjustment dial to < 6V30W > and

moving the “NCB11 filter” into the optical path results in

optimum color fidelity.

4. Point the optical path toward the camera.

Set the “optical path switchover dial

microscope to position < 4 >. (-> 20% light to the

observation port and 80% light to the front port)

 Supplementary informat ion

When the photographing device is attached to any port

other than the front port, distribute light to that port.

on the dia-illuminator into the optical

-DS dia-illuminator 30W”, setting

on the right side of the

II. Microscopy

5. Decide on the picture composition and focus on the film

surface.

1) Turn the “photo-mask dial” on the front side of the

microscope counterclockwise to move the photo-mask into

the optical path.

2) Turn the “fine/coarse focus knob” on the microscope to

bring the specimen into focus.

 Supplementary informat ion

Setting the “eyepiece tube turret” to position < M >

facilitates the focusing process. Take care not to touch

the “diopter adjustment ring” of the eyepiece in this

step.

3) Adjust the position of the specimen so that the target area

is located within the photo frame.

4) Move the “field diaphragm lever” on the dia-illuminator so

that the field diaphragm image is slightly larger than the

photo frame.

 Supplementary informat ion

The TE2000-S is not equipped with a photo-mask.

Look into the finder of the photographing device to

confirm the photographing range and focus.

Installation of the optional “mask eyepiece” enables

you to see the approximate photographing range and

focus on the film using the eyepiece.

Photo frame on photo-mask

For digital camera on the front port with T-BFA F-mount adapter (1X)

For 35-mm single-lens reflex camera on the front port with T-BSLR SLR camera adapter (2.5X)

For digital camera on the front port with T-BDCA direct C-mount adapter (2.5X)

View field of the mask eyepiece

PL2X (35-mm full size)

PL2.5X (35-mm full size)

PL2.5X (35-mm half size)

PL4X (35-mm full size)

PL5X (35-mm full size)

II. Microscopy

6. Adjust the brightne ss.

1) Move the “aperture diaphragm lever” on the condenser to

adjust the image contrast, depth of focus, and resolution.

2) Check the camera exposure time.

Longer than < 1/8 s > -> Acceptable

< 1/8 s > or shorter -> Move the “ND filter” on the dia-

illuminator in and out of the optical path to set the exposure

time to longer than < 1/8 s >. (-> To minimize the effect of

vibration caused by shutter operation)

 Supplementary informat ion

If precise color fidelity is not required, such as when

monochromic film is used, the brightness may be

reduced by using the “brightness adjustment dial” on

the left side of the microscope.

7. Prevent the entry of extraneous light.

1) Set the “eyepiece tube turret” to position < C >.

2) Install the finder cap to the camera finder or cover the

finder with a cloth.

 Supplementary informat ion

If an eyepiece tube turret is not included, place a cloth

on the eyepieces to prevent the entry of extraneous

light.

8. Press the shutter.

Using a remote release or self-timer of the camera eliminate s

the effect of vibration caused by shutter operation.

III. Operation of each part

In this chapter, operation of each part is described.

• Refer to “II. Microscopy” for the procedures on microscopy.

• If your microscope is not assembled yet, see “IV. Assembly” first.

• If you have mounted the Epi-fl or DIC attachment on the microscope, also refer to the instruction manual

supplied with the attachments.

• Also refer to “I. Parts of the microscope and their names” for simple description of each part.

• If you are using the TE2000-E model, read the instruction manual supplied with the T-HUBC

HUB controller.

• When the T-HUBC HUB controller is mounted on the rear side of the microscope, read the

instruction manual supplied with the HUB controller.

Before using the instrument, read the “ ”, “ ” and “Notes on handling the

microscope” at the beginning of this manual and be sure to follow the warnings and cautions written

therein.

Also refer to the instruction manual supplied with the other instruments used together with the

microscope (such as Epi-fl or DIC attachments) and be sure to follow the warnings and cautions

written therein.

III. Operation of each part

1. Power ON / OFF

Combination of the lamp, dia-illuminator and power supply

The combination of the dia-illuminator and the power supply is fixed against the ratings of the lamp to be used (either

12V100W or 6V30W). See the chart below to check the correct combination of the lamp, dia-illuminator and the power

supply.

No other combination can be used.

To control the lamp ON/OFF and lamp voltage adjustment from the remote control pad or PC via the T-HUBC HUB controller,

be sure to use the 12V100W lamp. The 6V30W lamp cannot be controlled using the remote control pad or PC.

Microscope

TE2000-

TE2000-E

TE2000-U

TE2000-S

Ratings

of the lamp

12V100W

Halogen lamp

OSRAM HLX 64623

PHILIPS 7724I

6V30W

Halogen lamp

PHILIPS 5761

Dia-illuminator

T-DH 100W

( LHS-H100P-1

12V100W lamphouse

is needed）

T-DS 30W

Power supply

On 100-120V areas On 220-240V areas

TE2-PS100W

(For 100-240V areas)

TE-PS30

(For 100-120V areas)

The remote control pad and PC cannot be used to control the lamp ON/OFF and lamp voltage adjustment.

TE-PSE30

(For 230V areas)

Power ON / OFF

There is a “power switch” at the front of the power supply.

Pressing the switch to “ | side” will turn ON the power. The pilot

lamp lights to show the power ON status. (If TE-PS30 or TE-

PSE30 power supply is used, the power switch itself lights

instead of a pilot lamp.) Dia-illumination can now be turned on

and off by the Dia-illumination ON / OFF switch on the side of the

microscope.

Pressing the power switch to “O side” will turn OFF the lamp and the

pilot lamp (or the switch itself).

* When the T-HUBC HUB controller is mounted on the

rear side of the microscope, be sure to read the

instruction manual supplied with the HUB controller.

Power switch

: On, O:Off

Dia-illumination ON / OFF switch

III. Operation of each part

2. Brightness adjustment

Brightness can be adjusted by ND filters (dimming filters) and/or the brightness adjustment dial.

Adjustment with ND filters

Filters that control light quantity are called ND filters. An ND

filter with a large number allows less light to pa ss and therefore

produces a darker image.

Since the ND filters will not change the tint (color temperature) of

the light source, they can be useful when color reproducibility is

of concern (such as when taking pictures using day-light type

color film.)

ND filters are to be set on the filter holders on the dia-illuminator.

ND2: Reduces light quantity to 1/2

ND16: Reduces light quantity to 1/16

ND filters

Adjustment with brightness adjustment dial

There are two brightness adjustment dials: one on the front of the power supply and the other on the left side of the

microscope base. Turning the dial changes the voltage supplied to the lamp, which changes the brightness and the hue of

the illumination.

To make the illumination bright, turn the dial to larger number which results in giving the bluish hue to the illumination. To

make the illumination dar k, turn the dial to smaller number which results in giving the reddish hue to the illumination.

On normal use, set the dial between 6 to 12.

If the color reproducibility is of concern (such as when taking pictures using day-light type color film), set the dial to the

same indication as the ratings of the lamp and place the NCB11 filter in the optical path. The illumination will then become

most similar to the white light. (*1)

Two dials cannot be used simultaneously. Select which dial is to

be used with the “EXTENAL switch (or CTRL switch)” on the rear

of the power supply. (*2)(*3)

*1: In this case, brightness adjustment should be performed

with ND filters.

*2: “EXTERNAL switch” is for TE2-PS100W power supply.

“CTRL switch” is for TE-PS30 or TE-PSE30 power supply.

*3: Note that the actual brightness differs slightly between the

same numbers on the brightness adjustment dial of the

power supply and the brightness adjustment dial of the

microscope.

• When the TE2-PS100W power supply is connected to

the T-HUBC HUB controller, be sure to refer to the

instruction manual supplied with the HUB controller.

EXTERNAL switch (CTRL switch)

Set to ON to use the brightness adjustment dial on the microscope.

Set to OFF to use the brightness adjustment dial on the power supply.

III. Operation of each part

3. Optical path switching

Use the “optical path switchover dial” to send the image to each port. (See page 51 for the type of the photomicrography

equipment that each port can accept.)

For TE2000-E

Connect the T-RCP remote control pad or PC to the T-HUBC HUB controller for the switching of the optical path. For details,

refer to the instruction manual supplied with the T-HUBC HUB controller.

When using the T-RCP remote control pad

For TE2000-U

*1: Dial position "3" cannot be used on

normal microscope models; however, an optional prism such as "front port 100%" and "right-side port 100%" can be installed.

Observation port

Observation port Right-side port

Observation port Front port

Bottom port

Left-side port

100

20 80

100

The LED above the selected key lights.

Position of

optical path

switchover

dial

1 100 - - 2 20 - 80 -

3 (*1) - - - -

4 20 80 - 5 - - - 100

Equipment that can be attached to

the port

Observation

port

Eyepiece tube

Intermediate

Tub e

trinocular

eyepiece tube

(+ various

equipment)

Light distribution(％％％％)

Front

port

SLR camera

F-mount

digital camera

Right-side

Photomicro-

equipment

microscopes

Digital camera

TV camera

port

graphy

for

Left-side

port

Photomicro-

graphy

equipment

for

microscopes

Digital camera

TV camera

III. Operation of each part

For TE2000-U Bottom port type

To open the optical path to the bottom port, pull out the bottom port changeover lever provided on the left-hand side. To

use this port, set the optical path switchover dial to position "3" and pull out the bottom port changeover lever.

Bottom port changeover lever

When the bottom port changeover lever is pulled out, the optical path is switched from the observation port to the bottom

port. If the bottom port is not used, be sure to press in the bottom port changeover lever. The optical paths to any other

ports can be switched in the same manner as for the TE2000-U.

For TE2000-S

Position of

optical path

switchover dial

SIDE 20 80

EYE 100 -

Equipment that can be

attached to the port

trinocular eyepiece

Light distribution(％％％％)

Observation

port

Eyepiece tube

Intermediate tube

tube (+ various

equipment)

Left-side port

TV camera

III. Operation of each part

4. Using filters

Set the appropriate filters on four filter sliders of the dia-

illuminator. The following filters are available.

Filter Description

ND2

ND16

NCB11

GIF

For brightness adjustment in normal microscopy and photomicrography.

Reduces light quantity to 1/2. (Approx. 50% transmissivity.)

For brightness adjustment in normal microscopy and photomicrography.

Reduces light quantity to 1/16. (Approx. 6% transmissivity.)

For correcting the color temperature in normal microscopy and color photomicrography using day-light type color film. (Should be removed on monochrome photomicrography.)

Color reproduction is optimized when this filter is set in the optical path and the brightness adjustment dia l is turned to the indication same as the ratings of the lamp.

Green interference filter. For microscopy using monochromatic light and for improving the contrast in monochrome photomicrography.

Heat absorption filter. For reducing the influence of heat rays in the illumination against specimen. Though the dia-illuminator has a built-in heat absorption filter, set this filter in the optical path if your specimen is very sensitive to heat.

Any filter

5. Using field diaphragm

The field diaphragm restricts the illumination on a specimen to

an observed area. Raising the field diaphragm lever to its

uppermost position will maximize the diameter of the diaphragm.

(*1)

For general use, the diaphragm should be set slightly larger than

the view field. Too wide an illuminated area gives off stray light,

which causes flares, resulting in reduced image contrast.

Therefore, for photomicrography in particular, correct

adjustment is very important. The best results can be obtained

by stopping down the diaphragm so that it is slightly wider than

the area to be imaged on the film (such as the frame on the

photo-mask). (Note that stopping down the diaphragm too far

causes vignetting.)

*1: The size of the field aperture diaphragm on “T-DS dia-

illuminator 30W” is fixed and cannot be changed.

Field diaphragm lever

III. Operation of each part

6. Using condenser aperture diaphragm

The condenser aperture diaphragm adjusts the numerical

aperture (N.A.) of the illumination system.

Aperture of this diaphragm determines optical resolution,

brightness, contrast, and depth of focus. Narrowing down the

condenser aperture diaphragm decreases resolution and

brightness, and increases contrast and depth of focus. Because

these characteristics are interrelated and cannot be controlled

one by one, the aperture must be adjusted for each specimen

and application.

Adjustment of the condenser aperture diaphragm is especially

important for bright-field microscopy, DIC microscopy, and

photomicrography. Generally, aperture settings of 70 to 80% of

the objective N.A. yield good images of appropriate contrast.

Adjust the aperture while observing the diaphragm image.

Moving the lever to the left stops down the diaphragm. Moving

the lever to the right opens it. Adjust so that the diaphragm

reaches 70 to 80% of the exit pupil of the objective.

Condenser aperture diaphragm lever

•••• T-TD eyepiece tube D or T-TERG ergonomic eyepiece tube:

Turn the eyepiece tube turret to “B” to move the Bertrand lens into the optical path. Turn the Bertrand lens

focusing scre w to observe an image of the objective’s exit pupil (bright circle) and the aperture diaphragm.

Objective pupil

Aperture diaphragm image

Turn to “B”.

70 80

100

•••• Other eyepiece tubes:

Remove one eyepiece and insert the “centering

telescope” with an adapter. Turn the eyepiece part of

the centering telescope to focus on the exit pupil of the

objective (bright circle) and the aperture diaphragm

image.

• When performing Ph microscopy with system condenser, be sure to fully open the aperture diaphragm. (If the aperture

diaphragm is stopped down, the optical path will be obstructed.)

• The aperture diaphragm on “ELWD-S condenser” works only on position A (for bright-field microscopy) and has no effect

on other positions (for Ph microscopy).

• There is no aperture diaphragm on “SLWD condenser”.

III. Operation of each part

7. Eyepiece tube turret

Position of the turret Description

OEmpty

The exit pupil of the objective can be observed. Turn the Bertrand

Bertrand

lens

C Shutter Shuts out extraneous light entering the eyepieces.

Eyepiece tube turret

• There is no eyepiece tube turret on “T-TS eyepiece tube S” and “T-TI intermediate tube”.

2.5X

magnifier

lens focusing screw to focus on the exit pupil.

If manipulator is used, this lens can also be used to observe the tip of the manipulator located just above the objective.

Applies 2.5x intermediate magnification only to the image seen through the eyepieces. Useful when slight enlargement is necessary or when performing focusing for photomicrography using 4 to 20X objective. (Not equipped on T-TERG ergonomic eyepiece tube.)

8. System condenser

A condenser has dual functions. It condenses light, and

provides the condensed light with optical elements to enable

various types of microscopic applications

Traditional microscopes require condensers to be changed

according to microscopic applications; for example, a Ph

condenser for Ph microscopy, and a DIC condenser for DIC

microscopy.

The system condenser of this microscope features modules that

contain the optical elements. Up to five modules can be

incorporated into the unit. As a result, the user needs only to

rotate the turret in order to execute various microscopic

applications, without changing condensers.

Modules can be arranged freely in the turret if they are applicable

to the condenser lens in use (there are 2 types of condenser

lenses). Modules may be replaced without dismounting the

condenser turret unit from the microscope, enabling several

microscopic applications to be executed for a short period of

time.

• For Ph microscopy, select the module having the same Ph code as marked on the objective. Bring it into the optical path

and center the annular diaphragm. Be sure to keep the condenser aperture diaphragm fully opened or the optical path

will be obstructed by the diaphragm.

• When using the T-CT-E motorized condenser turret, be sure to refer to the instruction manual supplied with the T-HUBC

HUB controller.

Condenser refocusing clamp

Condenser focus knob

urret and condenser module

Condenser lens

Aperture diaphragm lever

Condenser centering screw

•••• Specifications of the condenser lenses

LWD condenser lens ELWD condenser lens

N.A. 0.52 0.3

Working distance 30 mm 75 mm

Available microscopy BF, Ph, DIC BF, Ph

For BF: “A”

Usable condenser modules

Others

For Ph: “PhL”, “Ph1”, “Ph2”, “Ph3”

For DIC: “DIC L”, “DIC M”, “DIC H”

For HMC: “MC1”, “MC2”, “MC3”

* Condenser refocusing clamp can be used.

* Supplementary lens is not used with this

microscope.

9. Objectives

Ph objectives

III. Operation of each part

For BF: 「A」

For Ph: “PhL”, “Ph1”, “Ph2”

A Ph objective is labeled with a Ph code: PhL, Ph1, Ph2 or Ph3.

For Ph microscopy, use the annular diaphragm (condenser module) that has the same Ph code as the objective, regardless

of the magnification of the objective.

Objective with correction ring

An inverted microscope is frequently used to observe specimens through the bottom plate of a laboratory dish or culture

bottle made of glass or plastic. For such applications, the normal objectives (for 0.17 mm thick cover glass) may not

provide clear images, disabling the microscope from demonstrating its full performances. In such cases, use an objective

with a correction ring to compensate for bottom plate thickness.

Since the objectives with correction rings are not intended to compensate for wedge-like changes of thickness at edges of

a container, we recommend that they should be used for compensation for even thickness.

•••• Adjusting the correction ring

1) Turn the correction ring to match the bottom plate thickness of the container. The thickness should be a

measured value or the value stated by the manufacturer of the container. (The acrylic stage ring is available to

improve working efficiency since it enables the part of operation to be seen from above the stage.)

2) Focus on the specimen with the focus knobs.

3) If the image has poor resolution and/or contrast, slightly rotate the correction ring clockwise or counterclockwise.

When the correction ring is rotated, the specimen image becomes slightly out of focus. Focus on the specimen

again with the fine focus knob.

4) If this has improved the resolution and contrast, rotate the correction ring further in the same direction, then

adjust the focus again.

If the resolution and contrast are deteriorated, rotate the correction ring in the reverse direction double the

amount rotated in previous turn, then adjust the focus.

In this way, rotate the correction ring in the same direction if a better image is obtained , or rotate it in this reverse

direction if a poor image is obtained. Repeat this operat ion to find the best point.

5) Taking a note of the reading of a well-visible position on the correction ring will be a help when you later use

containers having different bottom plate thickness. (The 0 mm position of the correction ring is used for

microscopy of a specimen with no cover glass on an upright type microscopes.)

III. Operation of each part

Cover glass thickness

On every objective, there is a mark indicating the thickness of the cover glass to be used with. (“∞ / 0.17” means to use

a cover glass of 0.17 mm thick.) When observing with a “0.17” marked objective, place a specimen so that its cover glass

(0.17 mm thick) faces the objective. (In case of an inverted microscope, set a specimen so that its cover glass faces

down.) For an objective with a “1.2” mark, place a specimen so that its slide glass faces the objective, because the

normal slide glass thickness is 1.2 mm. (In case of an inverted microscope, set a specimen so that its cover glass faces

up.) When you observe a specimen in a laboratory dish or the like at high magnification through a glass not conforming

to the specified thickness, we recommend use of an objective that has a correction ring capable of correcting the glass

thickness error.

Oil immersion objective

The objective marked with “Oil” is an oil immersion objective.

Before using the oil immersion objective, be sure to immerse the space between the end of the objective and the specimen,

with the supplied oil (Nikon immersion oil). Use the non-fluorescent oil (option) when you carry out fluorescent

microscopy using the oil immersion objective for fluorescent microscopy.

Keep out air bubbles from the oil, since they will deteriorate visibility of images. Air bubbles may be found by observing

the exit pupil (bright circle) of the objective. (To observe the exit pupil of the objective, turn the eyepiece tube turret to

position “B” and focus with the Bertrand lens focusing screw. If your microscope does not have an eyepiece tube turret,

remove one eyepiece and insert the centering telescope with an adapter and turn the eyepiece part of the centering

telescope to focus.)

To remove air bubbles, rotate the revolving nosepiece slightly, and move the oil-immersed objective back and forth once

or twice. Or, wipe the oil off, then reapply oil to the objective.

If excessive oil is applied, surplus oil flows out and adheres to the stage or other component. Use a minimum necessary

amount of oil (enough to fill the space between the end of the objective and the specimen. Use care not to put oil to any

other components.

If oil remains on the oil immersion objective or adheres to the surface of a dry objective, it will greatly reduce image

visibility. After use, thoroughly wipe the oil off the objective surface. Also make sure that no oil has spread to the

surfaces of other objectives.

To remove oil, moisten a lens tissue or clean cloth with petroleum benzine and lightly wipe the lens surface a few times.

Use a fresh part of a lens tissue every time. For best results, wipe last with absolute alcohol (ethyl or methyl alcohol).

If you cannot obtain petroleum benzine, use methyl alcohol. Because methyl alcohol is a weaker cleaning agent, you will

need to wipe the surface repeatedly. (Usually, three or four wipes are adequate to clean the surface.)

Absolute alcohol and petroleum benzine are quite inflammable. Use great care when handling them and when setting the

power switch on and off. Be very careful with fire.

The use of acrylic stage ring (option) will facilitate the immersion operation with the culture dish left on the stage. Set the

stage ring so that its opening runs along the rim of the revolving nosepiece. Rotate the nosepiece till the objective shows

from the opening of the stage ring and hold the nosepiece there to immerse the top lens of the objective.

Water immersion objective

The objective marked with “WI” is a water immersion objective . (The one with longer working distance is for the upright

type microscopes.)

Before using the water immersion objective, be sure to immerse the space between the end of the objective and the

specimen, with the demineralized or distilled water. (Do not use the tap water for immersion since when it dries up,

impurities will stuck on the lens surface and may cause a scratch during cleaning.)

Plan Apo 60xWI objective (N.A. = 1.2) has a correction ring to compensate the difference of the cover glass thickness for

the best abberation compensation. The mark “17” on the graduation shows the position for the 0.17 mm thick cover glass.

Before using the cover glass, measure its thickness with the micrometer or the like, and set the correction ring to match

that thickness for the best compensation.

III. Operation of each part

10. Diopter adjustment

Perform diopter adjustment in the following way. The adjustment corrects dioptric difference of the left and right eyes,

facilitates observation with both eyes, and reduces defocusing when changing the objectives.

For TE2000-E, TE2000-U

Turn the “photo-mask dial” on the front of the microscope counterclockwise to place the photo-mask in the optical path.

Looking into the right eyepiece with your right eye, turn the diopter adjustment ring on the right eyepiece to focus on the

double crosshairs.

Then, looking into the left eyepiece with your left eye, turn the diopter adjustment ring on the left eyepiece to focus on the

double crosshairs.

For TE2000-S

Since there is no photo-mask on TE2000-S, the diopter adjustment cannot be performed in the same way as TE2000-U

which uses double crosshairs on the photo-mask.

1) Perform steps 1 to 4 on bright field microscopy described on page 25 so that the specimen is in focus with 10x

objective.

2) Turn the diopter adjustment rings of both eyepieces to align the engraved line on each diopter adjustment ring with

the rim of the objective outer ring. (This sets the diopter adjustment to the "0" position.)

3) Bring the 40x objective in the optical path.

4) Look into the right eyepiece with your right eye. Turn the focus knob to focus on the specimen.

5) Bring the 4x or 10x objective in the optical path.

6) Look into the right eyepiece with your right eye, turn the diopter adjustment ring on the right eyepiece to focus on

the specimen. (Do not touch the focus knob at this time.)

7) Repeat steps 3) to 6) twice.

8) Do the same for the left eyepiece. (Perform steps 3) to 6) three times substituting the word “right” with the word

“left”.)

11. Focusing module

•••• When using the TE2000-U/TE2000-S:

Never turn the focus knobs on the left and right sides of the microscope in opposite directions at the same time, as

doing so can result in damage to the microscope.

Turning the coarse focus knob as far as it will go and then attempting to turn it further will result in damage to the

microscope. Never use undue force to turn the knob.

The relationship between rotation of the knobs and movement of the objective is shown below. The fine/coarse

focus knobs provide a 7-mm upward and 3-mm downward strokes from the reference position.

See the below chart for the relationship between the rotation of the focus knobs and the amount of movement.

Amount of rotation

One graduation of fine focus knob One rotation of fine focus knob 0.1 mm One rotation of coarse focus knob 4.9 mm

Vertical movement of

objective.

1 μm

III. Operation of each part

• When using the TE2000-E:

The fine adjustment function of the focusing module of the TE2000-E is motorized, and the fine focus knob is

provided on the right side of the main body. The distance of movement achieved by one rotation of the fine focus

knob can be set to Fine, Middle, or Coarse. (The knob is set to Coarse at the factory.) The current Z-axis position

can be also displayed. For details, refer to the instruction manual supplied with the T-HUBC HUB controller.

The coarse focus knob on the left side of the main body is not motorized. Note that the coarse focus knob turns

during motorized Escape/Refocus movements. The relationship between the amount of coarse focus knob

rotation and the distance of objective movement is the same as in the TE2000-U.

 Supplementary information

The distance of movement achieved by one rotation of the fine focus knob is as follows. Note that these

distances are approximate and may vary depending on the knob-turning speed. The T-EFN focus knob

functions are identical.

Fine: 25 µm/rotation

Middle: 50 µm/rotation

Coarse: 100 µm/rotation

Also note that the distance per rotation of the coarse focus knob is 4.9 mm.

Coarse focus knob torque adjustment ring (not provided on TE2000-E)

Rotating the torque adjustment ring on the left side of the microscope counter-clockwise will make the rotation of the

coarse focus knob heavy.

Objective refocusing ring (for TE2000-U only)

The objective refocusing ring on the right side of the microscope is used to mark the position of the coarse focus knob

where a specimen is in focus.

Once the refocusing ring is clamped at the in-focus position, refocusing is much easier after shifting the objective for

specimen change or other purpose. All you have to do is rotate the coarse focus knob until it reaches the limit.

The refocusing ring is useful if, for example, the objective is very close to the stage because the container of the specimen

has a thick bottom plate, and the magnification can only be changed after lowering the objective.

When the refocusing ring is not used, be sure to turn it counterclockwise till the limit to loosen the clamp. If clamped, the

focusing mechanism cannot be moved up from the clamped position with the coarse focus knob. However, stage

movement with the fine focus knob is not affected.

1) At the position where a specimen is in focus, turn the refocusing ring clockwise to clamp the ring.

2) Lower the focusing mechanism by using only the coarse focus knob, and then change the objective.

3) Raise the focusing mechanism slowly to the limit, by using only the coarse focus knob. Here, the microscope is

roughly in focus. Then, rotate the fine focusing knob to give a sharp focus.

REFOCUS/ESCAPE switch (for TE2000-E only)

The REFOCUS/ESCAPE switches at the side of the focus knob on the right side of the microscope are used to mark the

vertical position of the objective when the specimen is in focus.

Pressing the ESCAPE switch (the lower switch) lowers the objective to the Escape position (2 mm below the standard

position) while memorizing the objective position at which the switch was pressed. Pressing the REFOCUS switch (the

upper switch) will raise and return the objective to the memorized position. Use these switches to facilitate refocusing

when the objective must be lowered for specimen changes and objective switching.

III. Operation of each part

• The fine focus knob is disabled at the Escape position.

• If the objective is at the Escape position, turning the coarse focus knob to move the objective position more than 0.5 mm

will clear the memorized position.

• Pressing the REFOCUS switch and ESCAPE at the same time will clear the memorized position.

• If the T-ND6-E sextuple motorized DIC nosepiece and the T-RCP remote control pad are also in use, automatic

Escape/Refocus movements can be set to correspond to objective switching. For detailed information, see the

instruction manual for the T-RCP remote control pad.

The coarse focus knob turns during Escape/Refocus movements.

•

coarse focus knob while these movements occur.

Turning the coarse focus knob will turn the motor set inside. On rare occasions, you may find that the coarse

•

focus knob fails to turn smoothly. This is because it engages the gear inside the motor. To restore smooth

movement, slightly turn the coarse focus knob in the opposite direction, then turn once again in the original

direction.

o prevent malfunctions, avoid contact with the

12. T-DH dia-illuminator 100W

Condenser refocusing clamp (can be used only when LWD condenser lens is attached)

After focusing the field diaphragm image on the specimen surface

using the condenser focus knob, turn the condenser refocusing

clamp clockwise to mark that position.

Even if the condenser is raised for specimen replacement, it can be

easily returned to the field diaphragm image in-focus position by

only lowering it to the limit. It is very useful when high N.A.

condenser lens is used or when the dia-illuminator cannot be tilted

for specimen replacement. The condenser refocusing clamp

works in range of 13 mm.

Condense

refocusing

clam

Rotating the condenser mount

To rotate the condenser mount, loosen the condenser mount

rotation clamp screw.

This function is mainly used for adjusting the vibration direction on

DIC microscopy.

When the system condenser is used and the polarizer is not

attached (such as when performing bright-field or Ph microscopy),

this function can be used to shift the condenser turret to the left or

right to make space for the manipulator or the other system.

Condense

mount

Condense

mount

rotation clamp

screw

III. Operation of each part

Tilting the pillar

The pillar can be tilted to provide a wider operation space which will be needed when changing a large-sized specimen.

Loosen the tilting clamp screw at the rear of the illuminator and hold the front part of the illuminating section to tilt the

illuminator backward.

Usually, the tilting clamp screw can be left unlocked.

When relatively heavy device is attached to the pillar, the tilting clamp screw should be locked to avoid the pillar from

accidental tilt.

Be careful not to get your fingers caught by the moving part of the dia-illuminator when tilting it.

•

When attaching relatively heavy devices to the dia-illuminator (such as high intensity light source and its

•

adapters), be sure to fix them tightly onto the illuminator or they will fall off when the illuminator is tilted.

Screw holes for mounting various devices

There are four M4 tapped holes on the front surface of the dia-illuminator. They can be used to fix manipulator and other

devices. The upper two holes are for the devices that should be moved away from the stage when the dia-illuminator is

tilted. The lower two holes are for the devices which should remain on the stage when the dia-illuminator is tilted.

13. T-SR rectangular stage

Normally, mount the stage so that its handle comes to the rear right of the microscope. It is also possible to mount the

stage in the diagonally opposite position, with the handle at the front left. Since the universal joint is adopted on the

handle, the handle can be adjusted to any angle.

There are tapped holes on the top and bottom plates of the stage, which can be used for mounting a manipulator or other

devices.

Two types of stage rings are available: 20 mm diameter and 40 mm diameter. Use them selectively according to the sizes

of specimen containers.

If the 40 mm stage ring is used, and the stage has been moved significantly out of observation range, the objective may

strike the bottom of the stage when rotated. In this case, first lower the nosepiece to the limit and then rotate it.

The stage rack protrudes during operation. When operating the focusing knobs or the revolving nosepiece, be

careful not to graze your hand on the end of the rack.

III. Operation of each part

14. Photomicrography

For details on assembly and usage of the camera and the photomicrography equipment, see the instruction manual

supplied with each equipment. Even if all microscope components are adjusted properly, you may find the image

vignetted in the finder of the camera. This has no influence on photomicrography, however.

Photomicrography equipment and ports that accept them

See the chart below for the relationship between photomicrography equipment and the port that accepts them.

Adapters need

Photomicrography equipment

Digital still cameras

(F-mount)

that have “aperture opening priority auto mode (A)” such as D1X, D1H, D1, etc.

35mm SLR camera

(F-mount)

that have “aperture opening priority auto mode (A)” such as FM3A, FE10, FE2, FE, F100, F90, F70, F801 series, F601 series, F501, F5, F4, F3, etc.

Digital still camera

for microscopes

DXM1200, DN100, etc.

Photomicrographic equipment for microscopes

U-III, H-III, P-III, etc.

Port that

accepts them

Front port

Side port

Bottom port

Side port

Observation port

(and its

intermediate

magnifi-

cation)

T-BFA F-mount adapter (1X)

T-BSLR SLR camera adapter (2.5X)

T-BDC A dir ect Cmount adapter

For side port:

T-BPA pho to adapter

For observation port:

Attach to the vertical tube of the trinocular eyepiece tube of the upright microscopes, mounted on the intermediate tube

Others

Set the intermediate magnification dial to "1.5X." If it is set to "1X," the edges of the view field become darker.

Needs PLI projection lens.

When attached to TE2000-S, use of “photo-mask eyepiece” will enable focusing through eyepiece.

When attached to the side port, the cover of the polaroid / 35mm camera back will not open fully.

The rectangular photo-mask (not equipped on TE2000-S) shows the range to be taken on photo when PLI 2.5X is used. When other PLI lenses are used, check the range through the finder on the photomicrographic equipment.

III. Operation of each part

Photomicrography procedure and items to be checked

On photomicrography, it is especially important to obtain appropriate illumination and focus the microscope properly.

First adjust the microscope for usual observation. Then, check and perform the following

Perform diopter

adjustment.

Adjust illumination.

Eliminate uneven illumination.

Switch the optical

path

4 Check the trimming.

Focus on the film

surface.

Shut out the

extraneous light.

Eliminate the influence of

vibration.

8 Make exposure.

For TE2000-E, TE2000-U:

For TE2000-S: Switching between 10x and 40x objective, use focus knob and diopter

Lamp voltage: For color photomicrography, set to the same indication as lamp ratings.

Filters: For color photomicrography, set NCB11 filter in the optical path.

Condenser: Perform focusing and centering of the condenser (if T-DH dia-

Camera: “+2/3” exposure adjustment will have good results when 35mm SLR

Use the optical path switchover dial, remote control pad, or PC to guide the image to the port on which the photomicrography equipment is attached.

(If this results in sending 100 % light to the specified port, you will need to switch back to the observation port frequently.)

For TE2000-E, TE2000-U:

For TE2000-S: Attach the “mask eyepiece” instead of an eyepiece and check, or look

For TE2000-E, TE2000-U:

For TE2000-S: Focus on the specimen while looking through the finder on the camera

If objective with correction ring is used, check the settings of the correction ring.

Field diaphragm: Close down to a size slightly larger than the photo frame.

Eyepieces: Turn the eyepiece tube turret (not equipped on T-TS eyepiece tube S

Finder on camera or photomicrography equipment:

If exposure time is shorter than “1/8s”, insert ND filters in the optical path to make the time longer than “1/8s”.

(If monochromatic film is used, you can also use lamp voltage to darken the image.)

Use release or self-timer of the camera.

Rotate the diopter adjustment rings so that both the photo-mask and the specimen is in sharp focus.

adjustment rings in turn. (See page 47 for details.)

Add color compensation filters if necessary.

illuminator 100W is used).

On Ph microscopy, perform annular diaphragm centering.

Aperture diaphragm is to be set to 70 to 80% of objective N.A. on normal use, and to be opened fully on Ph observation with system condenser.

camera is attached to the front port.

Use the photo-mask.

through the finder on the camera or photomicrography equipment.

Turn the focus knob so that both the photo-mask and the specimen image is in sharp focus. When using 4 to 40x objective, turn the eyepiece tube turret (not equipped on T-TS eyepiece tube S or T-TI intermediate tube) to “M” to enlarge the image on eyepiece only, and focus precisely. (Do not touch the diopter adjustment ring after turning the eyepiece tube turret to “M”.)

or photomicrography equipment, or through mask eyepiece.

or T-TI intermediate tube) to “C”. Or, cover the eyepieces with a piece of cloth.

Cover the finder with a piece of cloth or a finder cap. (Always leave the cover on, unless required to look into the finder.)

III. Operation of each part

Photo-mask and the range to be taken on photo

(Photo-mask is not equipped on TE2000-S.)

Turning the photo-mask dial counter-clockwise will bring photo-mask in the optical path. The phot o-mask can be used for

diopter adjustment or trimming on photomicrography.

For digital camera on the front port with T-BFA F-mount adapter (1X)

For 35mm single-lens reflex camera on the front port with T-BSLR SLR camera adapter

For digital camera on the front port with T-BDCA direct C-mount adapter

2.5X

Mask eyepiece and the range to be taken on photo

Remove one eyepiece and insert the “mask eyepiece” instead. This will enable trimming for photomicrography from the

binocular part of the microscope even with TE2000-S that does not have the eyepiece tube turret. It should be noted

however, that the range shown by the mask eyepiece is only a rough guide. If precise trimming is required, look and check

through the finder on the camera or the photomicrography equipment.

The following illustration shows the range taken on photo when the photomicrography equipment is attached on the side

port. (The range differs according to the magnification of the projection lens.)

PL2X (35mm full size)

PL2.5X (35mm full size)

PL2.5X (35mm half size)

PL4X (35mm full size)

PL5X (35mm full size)

IV. Assembly

• Before assembling the instrument, read the “ ”, “ ” and “Notes on handling the

microscope” at the beginning of this manual and be sure to follow the warnings and cautions written therein.

• To prevent electric shock and fire, turn off all the power switches of the system and unplug the power cords.

• Be careful not to pinch your hands or fingers during assembly.

• Scratches or fingerprints on lenses will adversely affect the image. Assemble the microscope so as not to put

scratches on the lenses.

• The microscope is a precision optical equipment. Handle carefully and do not subject it to strong shocks. (The

precision of the objectives in particular can be adversely affected even by weak shocks.)

• When using the TE2000-E

• When mounting the T-HUBC HUB controller

on the rear side of the TE-2000U

• When mounting the T-HUBC HUB controller

on the rear side of the TE-2000S

Also refer to the instruction manual supplied

with the T-HUBC HUB controller.

IV. Assembly

Required tools: 2mm hexagonal screwdriver x2 (supplied)

4mm hexagonal wrench x1 (supplied)

Installation location: Refer to the “Notes on handling the microscope” on the top of this manual and select an

appropriate location.

Condenser module

Condenser lens

Stage

Eyepiece

Eyepiece tube

Combination of "T-DH dia-illuminator 100W"

and "system condenser"

Condenser turret

Diailluminator

Condenser holder

Objective

Revolving nosepiece

Main body (base)

Filter slider

4 -1

Lamphouse

Lamp

4 -2

Combination of "T-DS dia-illuminator 30W"

and "ELWD-S condenser"

Filter

To wall outlet

Power supply

slider

Diailluminator

Condenser holder

Condenser

Lamp

Checking the power supply voltage

Make sure that the input voltage indicated on your power supply corresponds with the voltage of the

commercial power supply in your area. If the two values do not match, do not use the power supply, and

contact your nearest Nikon representative immediately. Use of a power supply with a different input

voltage indication can result in electric shock, fire, malfunction, and/or other accidents.

1. Setting the main body (base)

Take out the microscope base from the box and settle it on a stable desk.

The base is heavy. Hold it securely by the dent at the lower front and the carrying handle at the rear.

IV. Assembly

2. Eyepiece tube

1) Loosen the eyepiece tube clamp screw at the front of the base

using hexagonal screwdriver.

2) Remove the rubber cover.

3) Slide on the eyepiece tube from the front side till it hits the limit,

and then fix it by the clamp screw.

4) Put on the rubber cover.

5) Attach the eyepieces.

(Match 3 grooves on the eyepiece with 3 projections on the

eyepiece sleeve. The eyepieces on the right and left should be o

the same magnification. If you have rubber eye guards, put it on

the eyepieces.)

3. Revolving nosepiece

Attach the revolving nosepiece to the rectangular groove at the

center of the microscope.

Pressing the nosepiece from the front side to the rear, secure it by

two M5 hexagonal head bolts.

Screw on the objectives in an order that the magnification increases

as the nosepiece is turned clockwise when seen from above.

Eyepiece

Rubber

cover

Eyepiece tube

clamp screw

Press the nosepiece in this direction and secure.

Dia-illuminator is set here.

Bolts

4. Dia-illuminator

Remove the carrying handle from the base.

Place the dia-illuminator oo the rear mount. Make sure that the

positioning pins on the mount fit into the holes on the illuminator.

Fix the illuminator with four M5 hexagonal head bolts supplied together.

If “T-DH dia-illuminator 100W” is to be used, do the following also.

1) Attach the condenser mount

Attach the condenser mount sliding it from the bottom to the top

along the dovetail groove. (Slide it up until it hits the limit.)

Fix the condenser mount securely with the clamp screw on the

right side.

Attach the stopper screw to the illuminator.

When using a condenser lens other than the ELWD or ELWD-S,

loosen the clamp screw, slide the condenser mount down until it

makes contact with the stopper screw, then tighten the clamp

screw firmly.

2) Attach the lamphouse

Insert the lamphouse to the top part of the dia-illuminator.

Make sure that the positioning pin on the illuminator fits into the

groove on the circular mount of the lamphouse.

Securely fix the lamphouse by the clamp screw on the right side

of the illuminator.

Caution for heat: Do not touch while the lam is lit or immediately after the lamp is distinguished

Filter slider

Condenser

mount clamp

screw

Stopper screw

Lamphouse clam

screw

IV. Assembly

5. Filter slider

Attach the filters to the filter sliders. (Push aside the claw on the

circular mount when attaching the filter.)

Insert the sliders on the dia-illuminator.

6. Stage

Place the stage on the mounts at the center front of the base and the dia-illuminator.

(If your stage is equipped with the movement handle, let it position at the right rear of the microscope though it can also

be positioned at front left.)

Fix the stage with four M5 hexagonal bolt supplied together with the stage.

7. Lamp installation (lamp exchange)

• The combination of the lamp, dia-illuminator and the power supply is fixed. See page 38 and use them in

correct combination. Be sure to use the specified lamp.

• When exchanging the lamp, turn off the power and unplug the power cord to avoid electric shock.

• The lamp and its surroundings become extremely hot during lamp illumination and immediately after the

lamp is distinguished. When exchanging the lamp, turn off the lamp and wait till the lamp cools

sufficiently.

• Do not touch the lamp with bare hands. Dusts and fingerprints on the lamp surface will lead to uneven

illumination and shortening of the lamp life. Use gloves.

When using “T-DH dia-illuminator 100ＷＷＷＷ”:

Remove the cover of the lamphouse by loosening the clamp

screw on the right side of the cover.

Securely insert the lamp to the lamp socket till the limit while

pressing down the lamp clamp lever.

Slowly release the lamp clamp lever. Make sure that the lamp

is not tilted. If so, reattach the lamp.

Return the cover to the lamphouse and fix it by the clamp

screw.

Caution for heat: Do not touch while the lamp is lit or immediately after the lamp is distinguished

Cover clamp screw

Lamp clamp lever

When using “T-DS dia-illuminator 30ＷＷＷＷ”:

Open up the cover at the rear part of the dia-illuminator.

Attach the lamp to the socket.

Close the cover

Caution for heat: Do not touch while the lamp is lit or immediately after the lamp is distinguished

IV. Assembly

”

8. Condenser

When “T-DH dia-illuminator 100ＷＷＷＷ” is used:

(Attachable condensers:

System condenser or ELWD-S condenser)

Loosen the condenser clamp screw inside the hole at the

right side of the condenser holder using hexagonal

screwdriver. (If the mount has been rotated from its

standard position, the condenser clamp screw cannot be

found inside the hole. In the case like this, loosen the

condenser mount rotation clamp screw and rotate the

condenser mount with your hand so that the positioning

groove on the mount matches the positioning pin on the

condenser holder. Tighten the rotation clamp screw at that

position.)

Attaching the “system condenser”:

1) Rotate the turret so that the module “A

for bright-field microscopy) comes int o the optical path.

2) Slide on the turret to the bottom mount of the condenser

holder so that the indication “A” faces front. Fix the

turret securely by the condenser clamp screw.

3) Insert the modules to the turret and fix each of them by

two hexagonal screws. Five modules can be attached

to the turret. Set them so that the indication on the

module increases as the turret is rotated clockwise when

seen from above.)

4) Screw on the condenser lens to the bottom of the turret.

(See page 45 for the correct combination of the module

and the condenser lens. The auxiliary lens supplied

with the LWD condenser lens is not used on this

microscope.)

Attaching the “ELWD-S condenser”:

Insert it into the bottom mount of the condenser holder so

that the turret indication faces front. Fix it securely with

the condenser clamp screw.

(hollow module

Position ing pin

Position ing

roove

Mount rotation

clamp screw

Turret

Condenser

lens

Condenser module

Condenser clamp screw

(Deep inside the hole.)

Condenser clam

screw

When “T-DS dia-illuminator 30ＷＷＷＷ” is used:

(Attachable condensers:

ELWD-S condenser, SLWD condenser or HMC

condenser)

Attaching the “ELWD-S condenser”:

1) Attach the condenser holder on the dia-illuminator.

2) Attach the ELWD-S condenser on the condenser holder.

Condenser holder

ELWD-S

condenser

Attaching the “SLWD condenser”:

”

1) Remove the dustproof and field diaphragm sliders from

the illuminator.

2) Insert the SLWD condenser lens to the front, and Ph

annular diaphragm slider to the rear opening.

Attaching the “HMC condenser”:

1) Screw on the 52mm polarized filter inside the extension

tube supplied together with the HMC condenser lens.

2) Attach the extension tube to the dia-illuminator.

3) Attach the condenser holder to the extension tube.

4) Attach the condenser turret to the condenser holder.

5) Screw on the HMC condenser lens to the turret.

6) Attach the HMC module to the turret.

IV. Assembly

9. Power supply

First make sure that the power switch is turned off (pressed to the O side).

1) Lamp cable

Lamp cable of the dia-illuminator is to be connected to the

“OUTPUT” connector on the power supply. Do not forget to

tighten the lock ring on the connector.

2) Control cable

Connect one side the “LAMP CTR

and the other side to the “EXTERNAL” connector on the power

supply. (If TE-PS30 or TE-PSE30 power supply is used, connect

to “CTRL” connector instead of the EXTERNAL connector.)

When installing the HUB controller, be sure to refer to the

instruction manual supplied with the HUB controller.

3) Power cord

Connect one side to the “AC IN” connector on the power supply,

and the other side to the wall outlet.

If other devices, such as photomicrographic equipment, are to be

attached to the microscope, the power cord should be connected

only after those devices are attached to the microscope.

This ends the assembly of normal set.

connector on the microscope,

Power cord

(To the wall outlet)

Control cable

(To the “Lamp CTRL” connector on

the microscope)

Lamp cable

dia-illuminator)

(To the

IV. Assembly

”

10. Front port

See page 51 for the photomicrography devices that can be attached

to the front port and the adapters needed.

1) Pull off the plastic cap on the front port with your hand.

2) Insert the adapter and fix it by the clamp screw.

(If the adapter has a positioning pin, match it with the

positioning groove on the front port.)

3) Attach the photomicrography device to the adapter. have

rubber eye guards, put it on the eyepieces.)

Ex.: Attaching “Digital still camera D1

adapter”

Remove the cap on the F-mount adapter. Attach the

camera to the adapter matching the index marks, and

rotate the camera in the direction shown by the arrow

on the mount (counterclockwise) till a click is heard.

Dismounting the camera:

Pressing the lens dismounting button on the camera,

slowly rotate the camera clockwise until it stops.

Note that the F-mount may be damaged if you

attempt to rotate the camera in the direction of the

arrow.

to “T-BFA F-mount

Position ing

groove

Adapter clamp screw

11. Side port

See page 51 for the photomicrography devices that can be attached

to the side port and the adapters needed.

1) Remove the plastic cap on the side port by loosening the

clamp screw.

2) Insert the side port adapter and fix it by the clamp screw.

3) Insert the adapter to the side port adapter and fix it by the

clamp screw.

4) Attach the photomicrography device to the adapter.

Ex.: Attaching “Photomicrographic equipment U-III” to

“T-BPA photo adapter”

Insert the PLI projection lens to the “T-BPA photo

adapter” till it hits the limit. Insert the

photomicrographic equipment to the adapter so that

its finder faces forward. Fix it by tightening the

connection ring.

Side port

adapter

Side port adapter

clamp screw

12. Attaching various devices to the observation port

Remove the eyepiece tube from the microscope and attach the “T-TI intermediate tube” instead. The intermediate tube

accepts various devices such as the trinocular eyepiece tube for upright type microscopes and teaching head.

V. Troubleshooting

If the microscope does not function properly, take appropriate action as described below. If the problem is still not

resolved after referring to “Troubleshooting,” please contact your nearest Nikon representative.

1. Optical

Symptoms Causes Countermeasures

Install the parts (nosepiece, condenser, etc.) correctly.

Rotate the parts till the click stop position or till the limit. (Parts such as optical path switchover dial, nosepiece, filter sliders, condenser turret, eyepiece tube turret and photo-mask dial.)

Perform condenser focusing and centering correctly.

Adjust correctly.

Use container with bottom plate thickness within correction range.

Perform condenser focusing and centering correctly.

Put the annular diaphragm that matches the Ph code of the objective into the optical path.

Center the annular diaphragm.

Open fully.

Install correctly and rotate to the click stop position.

Center the annular diaphragm.

Adjust the brightness adjustment dial to match the lamp ratings.

View field vignetting.

Uneven brightness in the view field.

View field not visible.

Dirt or dust in the view field.

Poor image quality.

Poor contrast.

Poor resolution.

No phase contrast effect. (on Ph microscopy)

Uneven focus.

Image flows.

Yel lo w is h im age .

Parts not correctly installed.

Movable parts not switched correctly.

Stage ring on the optical path. Change the specimen position.

Field diaphragm image not focused on the specimen surface.

Field diaphragm stopped down too far. Open to slightly large a size as the view field.

Dirt or dust on lens and container. Clean them. Use clean container.

Field diaphragm image not focused on the specimen surface.

Dirt or dust on lens and container. Clean them. Use clean container.

Objective’s correction ring not set to the bottom plate thickness of the container.

Bottom plate thickness of the container is outside the correction range of the objective.

Field diaphragm image not focused on the specimen surface.

Bright-field objective is used. Use Ph objective.

Condenser annular diaphragm not on optical path.

Condenser annular diaphragm not centered.

Aperture diaphragm not fully open (when using system condenser).

Revolving nosepiece not installed correctly, or not rotated to the click stop position.

Specimen tilted against stage surface. Correctly reposition specimen on stage.

Revolving nosepiece not installed correctly, or not rotated to the click stop position.

Condenser annular diaphragm not centered.

Dia-illuminator tilted. Raise till it hits the limit.

No NCB11 filter in the optical path. Put it in the optical path.

Lamp voltage too low.

Ｖ

. Troubleshooting

Symptoms Causes Countermeasures

View field too bright。

View field too dark.

No ND filter in optical path. Put it in the optica l path.

Lamp voltage too high. Lower the voltage with brightness adjustment dial.

Condenser aperture diaphragm stopped down too far.

Field diaphragm image not focused on the specimen surface.

Optical path switchover dial not set to 100% observation.

Normally, set to 70 to 80% of the objective N.A.

Perform condenser focusing and centering correctly.

Switch to 100% observation.

2. Operational

Symptoms Causes Countermeasures

Image not in focus although the objective is raised to the highest position.

Cannot focus with 20x and 40x objective.

Images on the left and right eyepieces not coincident.

Eye fatigue

Stage mounted incorrectly. Mount it correctly.

Objective refocusing ring clamped.

Bottom plate thickness of the container is outside the correction range of the objective.

Interpupillary adjustment not performed. Make adjustment.

Diopter adjustment not performed. Make adjustment.

Inadequate brightness. Adjust with brightness adjustment dial or ND filters.

Release the clamp by turning the ring counterclockwise to the limit.

Use container with bottom plate thickness within correction range.

3. Electrical

Symptoms Causes Countermeasures

Power does not turn on even though the power switch is set to on.

Lamp does not light.

Lamp burnt too early.

adjustment dial on the microscope does not work.

ON/OFF switch on the microscope does not work.

Brightness adjustment dial on the power supply does not work.

No focus adjustment when the selector switch on the TEFN focus knob is switched on (only when the TE2000-E is used)

Power cord not connected, or connected improperly.

Lamp burnt. Replace with specified lamp.

Non-con forming lamp used. Replace with specified lamp.

Control cable not connected. Connect properly.Brightness

EXTERNAL switch on the power supply (CTRL switch when TE-PS30/TE-PSE30 is used) is OFF.

Lamp burnt. Replace with specified lamp.Dia-illumination

Control cable not connected. Connect properly.

EXTERNAL switch on the power supply (CTRL switch when TE-PS30/TE-PSE30 is used) is ON.

Knob connection cable is not connected or is improperly connected

Connect properly.

Turn ON.

Turn OFF.

Connect the cable properly.

V. Troubleshooting

VI. Care and maintenance

1. Lens Cleaning

Do not let dust, fingerprints, etc., get on the lenses. Dirt on the lenses, filters, etc., will adversely affect the view of

the image. If any of the lenses get dirty, clean them as described below.

• Either brush away dust with a soft brush, or else wipe it away gently with gauze.

• Only if there are fingerprints or grease on a lens, dampen a piece of soft, clean cotton cloth, lens tissue, or gauze

with absolute alcohol (ethyl or methyl alcohol) and wipe.

• Only when removing the immersion oil off from the objective, use petroleum benzine. If you cannot obtain

petroleum benzine, use methyl alcohol. However, because methyl alcohol does not clean as well as petroleum

benzine, it will be necessary to wipe the surfaces repeatedly. (Usually, three or four times is sufficient to clean the

lenses.)

• Never use petroleum benzine to clean the entrance lens at the bottom of the eyepiece tube or orusn surface of the

eyepiece tube.

• Absolute alcohol and petroleum benzine are highly flammable. Be careful when handling it, when around open

flames, when turning the power switch on / off, etc.

• Follow the instruction s provided by the manufacturer when using absolute a lcohol.

2. Cleaning the microscope

• We recommend that you use a silicon cloth to clean the microscope.

• For persistent dirt, dampen a piece of gauze with neutral detergent and wipe lightly.

• Using organic solvents could result in discoloration of the plastic parts.

3. Disinfecting the microscope

• We recommend that you use 70% medical alcohol for normal disinfection of the microscope.

• In case of spillage of a sample onto the microscope, determine whether the sample is hazardous. If the sample is

hazardous, follow your standard laboratory procedures.

• Using organic solvents could result in discoloration of the plastic parts.

4. Storage

Store the microscope in a dry place where mold is not likely to form.

Store the objectives and eyepieces in a desiccator or similar container with a drying agent.

Put the vinyl cover over the microscope to protect it from dust.

Before putting on the vinyl cover, turn off the power switch on the microscope (flip it to the O side) and wait until the

lamphou se is cool.

5. Periodical inspections (charged)

Periodical inspections (expenses charged) of this microscope are recommended in order to maintain peak

performance. Contact your nearest Nikon representative for details.

VII. Technical specifications

1. When the microscope (TE2000-E, TE2000-U, TE2000-S) is used in combination

with T-DH dia-illuminator 100W (for 12V100W lamp)

Combination Microscope + T-DH dia-illuminator 100W + TE2-PS100W power supply

Dimensions

Weight 41 Kg

Opto-mechanical specification

Input ratings of T-DH dia-illuminator 100W

Lamp ratings 12V100W halogen lamp

Lamp type Halogen lamp (OSRAM HLX 64623 or PHILIPS 7724I)

Average lamp lifetime 2,000 hrs.

TE2-PS100W power supply

Operating environmental conditions

Storage and transport environmental conditions

Installation category Category II

568(W) × 727(D) × 703(H) mm

Objectives: CFI60

Eyepieces: Field Number 22

Nosepiece : 6-place

Focusing:

Stroke 10 mm Coarse 4.9 mm/rev. Fine 0.1 mm/rev. Fine scale 0.05 μm (TE2000-E), 1 μm (TE2000-U,S)

DC 12 V, 100 W

Input ratings: AC 100-240 V, 50/60 Hz, 2.4 A

Voltage fluctuation: ±10%

Output ratings: DC 12 V, 100 W, 8.4 A

Built-in fuse ratings: 250 V, T4A

Protection class: Class 1

Others: UL Recognized product, GS approved

Altitude: 2,000 m max.

Temperature: 0 to 40ºC

Relative humidity: 85% max. (no condensation)

Pollution degree: Degree 2

Indoor use only

Temperature: -20 to +60℃

Humidity: max. 90% RH (no condensation)

VII. Technical specifications

2. When the microscope (TE2000-E, TE2000-U, TE2000-S) is used in combination with the T-DS dia-illuminator 30W (for 6V30W lamp)

When the supply voltage is 100 to 120 V:

Combination

Dimensions:

Weight: 37 Kg

Opto-mechanical specification

Input ratings of T-DS dia-illuminator 30W

Lamp ratings DC 6V30 W halogen lamp

Lamp Halogen lamp (PHILIPS 5761)

Average lamp lifetime: 100 hrs.

TE-PS30 power supply

TE-PSE30 power supply

Operating environmental conditions

Storage and transport environmental conditions

Installation category Category II

Microscope + T-DS dia-illuminator 30W + TE-PS30 power supply

When the supply voltage is 220 to 240 V:

Microscope + T-DS dia-illuminator 30W + TE-PSE30 power supply

568(W) × 727(D) × 611(H) mm

Objectives: CFI60

Eyepieces: Field Number 22

Nosepiece: 6-place

Focusing:

Stroke 10 mm Coarse 4.9 mm/rev. Fine 0.1 mm/rev. Fine scale 0.05 μm (TE2000-E), 1 μm (TE2000-U,S)

DC 6 V, 30 W

Input ratings: TE-PS30: AC 100-120 V, 50/60 Hz, 0.6 A

TE-PSE30: AC 230 V, 50/60 Hz, 0.4 A

Voltage fluctuation: ±10%

Output ratings: DC 6 V, 30 W

Built-in fuse ratings: 250 V, F2AH

Protection class: Class 1

Others: TE-PS30: UL Recognized product

TE-PSE30: GS approved

Altitude: 2,000 m max.

Temperature: 0 to 40ºC

Relative humidity: 85% max. (no condensation)

Pollution degree: Degree 2

Indoor use only

Temperature: -20 to +60℃

Humidity: max. 90% RH (no condensation)

VII. Technical specifications

3. Specified AC adapter for use with the HUB controller (only when using the T-

HUBC HUB controller)

Manufacturer PHIHONG ENTERPRISE (Taiwan)

Type PSA30U-120 (N)

Input rating AC 100-240 V, 0.7 A, 50/60 Hz

Voltage fluctuation

Output rating DC 12 V

Rated output current 2.5 A

Others UL Listed product, GS approved, CE satisfied

±10%

4. Power cord for power supply and for AC adaptor of HUB controller

Be sure to use the following power cords.

When the supply voltage is 100 to 120 V

When the supply voltage is 220 to 240 V

UL Listed detachable cord set, 3 conductor grounding Type SVT, No. 18 AWG, 3 m long maximum, rated at 125 V AC minimum.

Approved according to EU/EN standards, 3 conductor grounding Type HO5VV-F, 3 m long maximum, rated at 250 V AC minimum.

VII. Technical specifications

5. Conforming standards

Microscope (TE2000-E,

TE2000-U,

TE2000-S) used in combination with the TE2-PS100W power supply or TE-PS30 power supply

UL Listed Product.

FCC 15B, Class A satisfied.

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.

This Class A digital apparatus complies with Canadian ICES-

003.

Cet appareil numérique de la classe A est conforme à la norme NMB-003 du Canada.

* Note that the microscope will not meet the above standards if used in combination with a motorized XY stage or shutter

made by any manufacturer other than Nikon.

・ Meets EU IVD Directive (In vitro

diagnostic medical device directive)

Microscope (TE2000-E,

TE2000-U,

TE2000-S) used in combination with TE2-PS100W power supply or TE-PSE30 power supply

requirements. (GM-approved: In vitro diagnostic medical device)

・ Meets EU LV Directive (Low voltage

directive) requirements.

・ Meets EU EMC Directive requirements.

(EN61326)

* Note that the microscope will not meet the above standards if used in combination with a motorized XY stage or shutter

made by any manufacturer other than Nikon.

VII. Technical specifications

Blank Page

System diagram

HMC MC1, MC2, MC3

Centering Telescope

Y-TT Trinocular Tube TUW

Y-TF Trinocular Tube FUW

Y-IDT Drawing Tube

Teaching Heads

Eyepiece Tubes

Modules

Phase PH-L, PH-1, PH-2 Modules

Phase PH-L, PH-1, PH-2, PH-3; DIC NL, NM, NH; HMC MC1, MC2, MC3 Modules

HMC Lens

ELWD Lens

LWD Lens

System Condenser

Turret

T-CT-E Motorized Condenser Turret

Eyepiece Tube D

C-HU Universal Holder

Acrylic Stage Ring

T-SR Rectangular Stage

C-Mount

CCTV

Camera

C-Mount Zoom Adapter

ENG-Mount

CCTV Camera

T-TD

C-HT Terasaki

Photomicrographic System FX-III Series

Multi-image Module

C-Mount Adapter A

C-Mount Adapter

0.45X, 0.6X

C-Mount CCTV Adapter

TV Zooming Lens

Adapters

T-TS

Eyepiece Tube S

C-HSG Slide Glass Holder

T-BPA Photo Adapter

C-Mount Adapter A

C-Mount TV Adapter VM4X

C-Mount TV Adapter VM2.5X

T-BPA

Relay

Photo

Lens 1X

Adapter

T-TERG

Ergonomic

EyepieceTube

Hemacytometer Holder

T-SP Plain Stage

35mm SLR Camera

High-definition Digital Camera DXM1200

C-mount TV Camera

F-mount Digital Camera D1

T-TI

Intermediate

Tube

35mm Petri

Dish Holder

T-SAM Attachable Mechanical Stage

T-FLBW-E BA Filter Wheel

T-BSLR Front-port SLR Adapter

T-BFA Front-port F-mount Adapter

T-BDCA Front-port Direct C Adapter

Stages

Side Port

Front Port

DIC NM, NH Modules

DIC NH, NH-SS Modules

T-N6 Sextuple

Nosepiece

Eyepiece Tubes

REFOCUS

COARSE FOCUS

ESCAPE

High N.A. Lens

(Dry)

High N.A. Lens

(Water)

High N.A. Lens

(Oil)

CFI Objectives

T-ND6 Sextuple

DIC Nosepiece

Nosepieces

T-BSUK Stage Riser

Stages

Nosepieces

Attachments

A B

Epi-fl

Bottom Port

High N.A.

Condenser

Holder

High N.A. Condenser

TE-C ELWD Condenser

DIC Slider

T-ND6-E Sextuple

Motorized DIC Nosepiece

Stage Riser

Illumination

Pillars

Lens Unit

T-BSUK

T-HUBC

Hub Controller

Side Port

T-A Analyzer

T-A-E Motorized DIC Analyzer

T-AC AC Adapter

T-RCP

Remote Controller

Condensers

Lambda Plate

T-P Polarizer

Condensers

T-DH Dia-illuminator 100W

Epi-fl Attachments

Eyepiece

Stages

Tubes

Filter 45mm; GIF, NCB11, ND16, ND2 Heat Absorbing

T-FLC Cassette Holder

T-FLC-E

Motorized Cassette Holder

Nosepieces

A B

TE2-PS 100W

12V Power Supply for

100W Illumination Pillars

Epi-fl Filter Blocks

T-FL-E Epi-fl Attachment with Motorized Shutter

Illumination

Pillars

HMX

Lamphouse

Adapter

LHS-H100P-1 Lamphouse

Remote

Cable

T-FL Epi-fl Attachment

Eyepiece Tubes

Collector

Lens

Illumination Pillars

FL Illumination

Adapter

Illumination

Stages

A B

Halogen Lamp 12V-100W

Halogen Lamp

12V-100W LL

T-FLA

T-FLZA

FL Zoom

Adapter

Nosepieces

Halogen Socket 100W

HMX Lamphouse

System

Condenser

HMC MC1, MC2, MC3

T-FLEW-E

EX Filter Wheel

Illumination

Pillars

SLWD Condenser

Turret

HMC Lens

Modules

TE-AT Double

Lamphouse

Adapter

TE-C ELWD

Condenser

C-FC Epi-fl

Collector Lens

Epi-fl

Collector

Lens Q2

Filter 33mm; GIF, Heat Absorbing, NCB11, ND16, ND2

Halogen Lamp

6V-30W

T-DS Dia-illuminator 30W

Lamphouse

HMX-3B/HMX4B

w/Backmirror

Xenon

Lamphouse

System diagram

TE-PS 30/TE-PSE30 6V Power Supply for 30W Illumination Pillar

Remote

Cable

Provided with the HMC lens.

Mercury Lamp Socket S 100W

Xenon

Lamp Socket

Marcuy Lamp HG-100W

Starter HG-100W

Xenon Lamp

Xenon Starter

Side Port

Epi-fl

Attachments

Side Port

Epi-fl

Attachments

Front

Port

Model with bottom port also available

• Components not included in UL or GM approval are also shown.

• Components not described in this manual are also shown.

• This system diagram is subject to change without notice.

HMX

Lamphouse

Halogen Socket

100W

Halogen Lamp 12V-100W

U Transformer

Nikon TE2000-E, TE2000-U, TE2000-S User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual