Nikon Eclipse Ti-E, Eclipse Ti-E/B Instructions Manual

M450 E 07.10.NF.1

Inverted Microscope

Instructions

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of Nikon Inverted Microscope Eclipse Ti-E and Ti-E/B.

To ensure correct usage, read this manual carefully before operating the product.

• No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

• The contents of this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may

remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative.

• Some of the equipment described in this manual may not be included in the set you have purchased.

• If you intend to use any other equipment with this product, read the manual for that equipment too.

• When using TI-HUBC/A Hub Controller A with the microscope, also refer to the instructions manual

provided with the hub controller.

• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

Warning and Caution Symbols in this Manual

Although this product is designed to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

Warning

Caution

Disregarding instructions marked with this symbol may lead to serious injury or death.

Disregarding instructions marked with this symbol may lead to injury or property damage.

Symbols on the Product

The symbols on the product indicate the need for caution at all times during use. Before operating a part labeled with the following symbols, refer to the instruction manual and read the relevant instructions.

Symbol Meaning

CAUTION: HOT

This symbol can be found on the top of the dia pillar illuminator and on the 12V 100W lamphouse, and cautions the following:

• The lamp and the lamphouse will be extremely hot while and immediately after using the lamp.

• To avoid the risk of burns, do not touch the lamp and the lamphouse while or immediately after using the lamp.

• When replacing the lamp, wait for the lamp and the lamphouse to cool sufficiently.

Biohazard

This symbol can be found on the upper part of the microscope, and cautions the following:

• The product may become biohazardous if a specimen is spilled onto the product.

• To avoid exposure to biohazard, do not touch contaminated parts with your bare

hands.

• Decontaminate the contaminated parts according to the standard procedures for your facility.

General Caution

This symbol can be found on the top of the nosepiece protection plate on Ti-E and Ti-E/B, and on top of the PFS6 nosepiece protection plate on the TI-ND6-PFS PFS Motorized Nosepiece, and cautions the following:

• To avoid injury (i.e. by getting your fingers caught), be sure to attach the protection plate correctly.

Safety Precautions

LED Safety

The PFS Motorized Nosepiece (TI-ND6-PFS Perfect Focus Unit) uses light in the near-infrared region (IR wavelength) emitted from an infrared LED to control the focus. When using the PFS Motorized Nosepiece with the product, the microscope system conforms to the EU standard EN60825-1: 2001 and the international standard IEC60825-1: 2001. The product is classified as a Class 1 LED product.

Attempting to control or adjust the product in manners not described in this manual may result in exposure to hazardous LED light.

Safety Labels on TI-ND6-PFS Perfect Focus Unit

• Safety labels are affixed to the bottom of the TI-ND6-PFS Perfect Focus Unit.

(3) Safety standards label

TI-ND6-PFS

JAPAN

593901

CLASS 1 LED PRODUCT

IEC/EN60825-1 : 2001

CAUTION - CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTRLY WITH OPTICAL INSTRUMENTS

(2) Class1 LED

product label

(1) Caution label

CAUTION - CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTRLY WITH OPTICAL INSTRUMENTS

(2) Class 1 LED product label

CLASS 1 LED PRODUCT

IEC/EN60825-1 : 2001

(3) Safety standards label

PFS Motorized Nosepiece (bottom view)

TI-ND6-PFS

JAPAN

593901

• Note that under normal use, infrared light is emitted from the position shown in the figure below.

Infrared light emission port

OUT

DICHROIC MIRROR

Safety Precautions

1. Intended application of the product

Warning

The product is intended mainly for microscopic observations and micromanipulations of living cells and tissues under diascopic or episcopic illumination. It is designed for the purposes of experimentations and observations at hospitals or laboratories in the fields of genetics, immunology, physiology, pharmacology, neurology, cellular biology, and molecular biology.

The product is classified as an in-vitro diagnostic medical device.

2. Do not disassemble.

Disassembling this product may result in electric shock or malfunction. Malfunctions and damage due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated in this manual. If you experience problems with the product, contact Nikon.

3. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used with this product. In particular, be sure to follow the warnings and cautions at the beginning of the manuals.

4. Check the input voltage.

The microscope body uses an AC adapter as its power source. The dia illumination lamp uses one of two types of power supply devices.

• AC adapter for the microscope body:

The AC adapter for the microscope can be used with 100 to 240 VAC at 50-60 Hz, and can be used with most wall outlets in the world. Under normal use, you will not need to pay particular attention to the supplied power voltage.

• TI-PS100W Power Supply: TI-PS100W Power Supply can be used with 100 to 240 VAC at 50/60 Hz, and can be used with most wall outlets in the world. Under normal use, you will not need to pay particular attention to the supplied power voltage.

• TE-PS30W Power Supply A or TE-PSE30 Power Supply A: The input voltage ratings are indicated on the rear panel of the power supply device. Before connecting the power cord, check that the indicated input voltage matches the voltage of the wall outlet. If the indicated input voltage does not match your regional voltage supply, do not use the power supply device, and contact Nikon. Use of a power supply device with the inappropriate voltage rating may result in overheating or fire due to overcurrent, and may also cause damage the power supply device and connected devices.

5. Use the specified AC adapter only.

The product is powered by an AC adapter. Be sure to use the specified AC adapter with the product. Use of other AC adapters may result in malfunction, overheat, or fire.

• See Chapter 7, “Specifications” for the specified AC adapter.

• Place the AC adapter in a well-ventilated area to prevent malfunction and fire. Obstacle

covering or placed on the AC adapter may hinder the dissipation of heat and cause the AC adapter to become abnormally hot.

• To prevent malfunctions and errors, turn off the POWER switch on the Ti-E or Ti-E/B microscope body (set the POWER switch to the “OFF” side) before connecting the AC adapter.

Safety Precautions

6. Cautions on the power cord

Warning

Be sure to use the specific power cords for the AC adapter and the power supply device. Use of other power cords may result in malfunction, overheat, or fire.

• See Chapter 7, “Specifications” for the specified power cord.

• To prevent electric shock, turn off the power switches on the Ti-E or Ti-E/B microscope body

and on the power supply device before connecting or disconnecting the power cord. On Ti-E and Ti-E/B, set the POWER switch to the “OFF” side. On the power supply, set the POWER switch to the “O” side.

• Note that the power supply device is classified as Class I for electric shock protection. Be sure to connect it to a protective ground terminal.

7. Check the combination of dia pillar illuminator, lamp, and power supply device.

The combination of the dia pillar illuminator and the power supply device is specified based on the lamp ratings (12V/100W or 6V/30W) and the power voltage. Use them in the correct combination according to the instructions on page 55. Use of the devices in a wrong combination may result in malfunction, overheat, or fire.

8. Cautions on heat from the light source

The lamp and the lamphouse become extremely hot when the lamp is turned on. Follow the cautions below to prevent burns and fire.

• To avoid burns, do not touch the lamp and the lamphouse while the lamp is on or for approximately thirty minutes after it has been turned off.

• To avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (i.e. gasoline, petroleum benzine, paint thinner, and alcohol) near the lamphouse while the lamp is on or for about thirty minutes after it has been turned off.

• Do not block the air vents on the lamphouse. If any obstacle covers the lamphouse or something is placed on the lamphouse, the heat dissipation is inhibited and the lamphouse becomes abnormally hot.

• The bottom of the power supply device becomes hot during use. Do not cover the air vents on the side of the power supply device.

9. Cautions on lamp replacement

• When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and

make sure that the lamp and the lamphouse have cooled sufficiently.

• To prevent electric shock and product damage, turn off the power switch on the power supply (set the POWER switch to the “O” side) and unplug the power cord from the wall outlet before replacing the lamp.

• After replacing the lamp, close and secure the lamphouse cover. Never use the product with the lamphouse cover left open.

• Do not break the used lamp. It should be disposed of as an industrial waste, according to the local regulations and rules.

Safety Precautions

10. Cautions on motorized devices

Warning

The product can rotate and focus (raise and lower) the PFS Motorized Nosepiece with motors. The product can also have various motorized devices attached. When TI-HUBC/A Hub Controller A is used with the product, the system can be controlled with a TI series controller or a PC.

To prevent injuries, pay attention to the following when operating motorized devices:

• Before operating the product, check the whole microscope system, and make sure that all moving parts can be operated safely.

• Your hands and fingers may be caught and injured by the nosepiece, objectives, stage, parts arranged on the stage, or specimen containers. Keep your hands away from these devices during operation.

• Check the optical path of the whole microscope system before operating the shutter on the illuminator or adjusting the brightness. The light source emits a very strong light. If the optical path is not set up correctly, the illumination light may leak out or enter the objective, and cause an eye injury.

11. Notes on handling hazardous specimens

The product is intended mainly for microscopic observations and micromanipulations of specimens such as living cells and tissues in a Petri dish. Note the following when handling the specimens:

• Before handling a specimen, check whether it is hazardous. Wear rubber gloves when handling hazardous (i.e. potentially infectious) specimens.

• Be careful not to spill the specimen. If a specimen is spilt onto the microscope, decontaminate according to the standard procedures for your facility.

12. Notes on handling flammable solvents

The following flammable solvents are used with the product:

• Immersion oil (Nikon Immersion Oil for oil immersion objectives)

• Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

• Petroleum benzine (for wiping off the immersion oil)

• Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions provided by the manufacturer, and handle correctly and safely. Note the following precautions when using solvents with the product.

• Keep solvents away from the lamp, the lamphouse, the power supply device, and any other parts that may become hot.

• Keep solvents away from the product and its surroundings when turning on/off the power switch or plugging/unplugging the power cord.

• Be careful not to spill the solvents.

Safety Precautions

1. Turn off the power during installation, assembly, connection/disconnection of cables,

Caution

and maintenance.

To prevent electric shock and fire, be sure to turn off the power switch on the microscope body and the power supply device (set the POWER switches to the “OFF” side or the “O” side) and unplug the power cords before installing or assembling the product, connecting or disconnecting cables, replacing the lamp, or performing maintenance tasks such as cleaning of the lenses.

2. Do not wet the product or allow intrusion of foreign matters.

Do not wet or spill liquids onto the product, as it may result in malfunction, overheat, or electric shock. If water or other liquids are accidentally spilled onto the product, immediately turn off the power switch (set the POWER switch to the “OFF” side or the “O” side) and unplug the power cord from the wall outlet. Then, wipe off the liquid with a piece of dry cloth. Intrusion of foreign matters into the product may also result in malfunctions. If liquids or foreign matters enter the product, do not use the product, and contact Nikon.

3. Weak electromagnetic waves

The product emits weak electromagnetic waves. Do not install the product near precision electronic devices to avoid affecting their performance. If signal reception by a TV or radio is affected, move the TV or radio set away from the product.

4. Cautions on moving the product

• When carrying the product, hold the product firmly by the bottom front recess and the bottom

rear.

• When moving the product, do not hold by the focusing knobs, eyepiece tube, stage, or dia pillar illuminator. The parts may become detached and cause the product to fall, and may also result in malfunctions and loss of precision.

5. Cautions on assembling the product

• Take care to avoid pinching your fingers and hands.

• Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will

degrade the microscope image. When assembling the product, be careful not to scratch or directly touch the optical components.

6. Cautions on the protruding rack of the Rectangular Mechanical Stage

The stage rack of TI-SR Rectangular Mechanical Stage will protrude when the stage is operated. When operating the focus knobs or the nosepiece, be careful not to strike your hands against the rack. Contact with the edges of the rack may result in injury.

7. Disposal

To avoid biohazard risks, dispose of the product as contaminated equipment, according to the standard procedures for your facility.

Safety Precautions

Notes on Handling the Product

1. Handle with care.

The product is a precision optical instrument. Handle the product with care and avoid physical shocks and vibrations. In particular, the precision of objectives may be lost by even weak physical shocks.

2. Installation location and storage location

The product is a precision optical instrument. Use or storage of the product under inappropriate conditions may result in malfunctions or loss of precision. The following conditions must be considered for the installation location and the storage location.

• Install the product in a location with a temperature of 0 to 40°C and a relative humidity of 85% or less (no condensation). Store the product in a location with a temperature of -20 to 60°C and a relative humidity of 90% or less (no condensation). Use or storage of the product in a hot or humid location may result in molding of or condensation on the lenses, loss of precision, and malfunctions.

• Install the product in a place with little dust and dirt. When storing the product, place a cover over the product to protect it from dust.

• Install the product in a place with little vibration.

• Install and store the product on a level and sturdy table or stage that can bear the weight of the

product. Install the product in a location with minimal exposure to hazards in the event of earthquakes and other potential disasters. If necessary, secure the product to the working desk or other heavy and stable items with a strong rope or other means, so as to prevent it from falling.

• Avoid placing the product in direct sunlight or immediately under the room lights. The image quality is degraded in a bright environment due to the extraneous light entering the objective. A room light immediately above the microscope may also enter the objective as extraneous light, especially when using a condenser lens with a longer working distance (i.e. ELWD and LWD). In this case, it is recommended that you turn off the room light above the microscope.

• Install the product at least 10 cm away from the surrounding walls. When using TI-DH Dia Pillar Illuminator 100W, install the product at least 15 cm away from the wall, so that the caution labels on the dia pillar illuminator and the lamphouse are visible. Note that TI-DH Dia Pillar Illuminator 100W can be inclined backward to secure working space. To utilize this function, install the product so that there is enough space between the product and the wall for the dia pillar illuminator to be inclined.

• Do not install the product in a closed space such as a locker or a cabinet.

• Do not place items on the product.

• Install the product so that the power cords to the AC adapter and the power supply can be

unplugged immediately in case of an emergency.

3. Notes on handling optical parts

Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will degrade the microscope image. Handle the optical components with care, so as not to damage them. If they require cleaning, see Chapter 6, “Daily Maintenance.”

4. Notes on handling the lamp

Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp may result in uneven illumination and reduce the service life of the lamp. Wear gloves or use other pieces of clothes when handling the lamp.

Safety Precautions

5. Notes on using the focus knobs

• Never rotate the left and right focus knobs on the microscopes in the opposite directions at the same

time. Doing so may damage the product.

• Do not rotate the focus knobs past their limit. Doing so may damage the product.

6. Protecting the ports.

The microscope has multiple ports. To keep out extraneous light and dust, attach the provided caps to the ports that are not in use.

7. Caution on motorized devices

Do not force movements on the motorized parts by hand.

8. Note on vibrations caused by motorized operations

This product is designed to minimize the amount of vibration during motorized operation. However, depending on the application, the vibration may have an effect on the microscopy.

9. Note on the Z-axis position display

Ti-E and Ti-E/B microscopes can display the Z-axis position on the front status display panel. Note, however, that the precision of the value is not guaranteed, as Ti-E and Ti-E/B are not designed as measurement machines.

Introduction...................................................................................................................................................... 1

Safety Precautions .......................................................................................................................................... 2

Warning and Caution Symbols in this Manual............................................................................................................ 2

Symbols on the Product ............................................................................................................................................. 2

LED Safety ................................................................................................................................................................. 3

Notes on Handling the Product ..................................................................................................................................8

Contents ..........................................................................................................................................................11

1. Part Names ............................................................................................................................................. 12

1.1 Ti-E, Ti-E/B.................................................................................................................................................... 13

1.2 Microscope Base .......................................................................................................................................... 14

1.2.1 Microscope Base............................................................................................................................ 14

1.2.2 Operation Panels............................................................................................................................ 15

1.2.3 Connector Panels........................................................................................................................... 16

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces.................................................................................... 17

1.4 PFS Motorized Nosepiece and PFS Offset Controller ..................................................................................19

1.5 Stage.............................................................................................................................................................20

1.6 Dia Pillar Illuminator ...................................................................................................................................... 21

1.6.1 TI-DH Dia Pillar Illuminator 100W................................................................................................... 21

1.6.2 TI-DS Dia Pillar Illuminator 30W..................................................................................................... 21

1.7 Condenser ....................................................................................................................................................22

1.7.1 TI-C Condenser Turret (System Condenser).................................................................................. 22

1.7.2 ELWD-S Condenser ....................................................................................................................... 23

1.8 Power Supply................................................................................................................................................ 24

1.8.1 TI-PS100W Power Supply.............................................................................................................. 24

1.8.2 TE-PS30W Power Supply A (for 100-120V) TE-PSE30 Power Supply A (for 220-240V) ............... 25

1.9 AC Adapter ................................................................................................................................................... 26

2. Microscopy............................................................................................................................................. 27

2.1 Introduction to Microscopy ............................................................................................................................ 28

2.2 Bright-Field (BF) Microscopy......................................................................................................................... 30

2.3 Phase Contrast (Ph) Microscopy ..................................................................................................................38

2.4 External Phase Contrast Microscopy ............................................................................................................ 43

2.5 In-focus Observation with PFS......................................................................................................................49

3. Operation................................................................................................................................................ 53

3.1 Power On/Off ................................................................................................................................................ 54

3.1.1 Power On ....................................................................................................................................... 54

3.1.2 Power Off ....................................................................................................................................... 54

3.2 Dia Illumination Operation............................................................................................................................. 55

3.2.1 Combination of Lamp, Dia Pillar Illuminator, and Power Supply..................................................... 55

3.2.2 Power Supply Setting ..................................................................................................................... 55

3.2.3 Dia Illumination Lamp Operation .................................................................................................... 56

3.2.4 Brightness Adjustment with ND Filters ...........................................................................................56

3.3 Controls on the Microscope Body ................................................................................................................. 57

3.3.1 Front Operation Panel .................................................................................................................... 57

3.3.2 Left Operation Panel ......................................................................................................................59

3.3.3 Right Operation Panel .................................................................................................................... 60

3.4 Optical Path Selection................................................................................................................................... 61

3.4.1 For Ti-E ..........................................................................................................................................61

3.4.2 For Ti-E/B....................................................................................................................................... 61

3.4.3 Eyepiece Base Unit Options........................................................................................................... 61

3.5 Filter Operation ............................................................................................................................................. 62

3.6 Field Diaphragm Operation ........................................................................................................................... 63

3.7 Aperture Diaphragm Operation..................................................................................................................... 64

3.8 Condenser Operation.................................................................................................................................... 66

3.8.1 TI-C Condenser Turret (System Condenser).................................................................................. 66

Contents

3.8.2 ELWD-S Condenser ....................................................................................................................... 67

3.9 Eyepiece Tube Operation .............................................................................................................................68

3.9.1 Diopter Adjustment ......................................................................................................................... 68

3.9.2 Interpupillary Distance Adjustment ................................................................................................. 68

3.9.3 Eyepiece Tube Shutter Operation .................................................................................................. 69

3.9.4 Bertrand Lens Operation ................................................................................................................ 69

3.10 Focusing Mechanism Operation ...................................................................................................................70

3.10.1 Focus Knob Operation ...................................................................................................................70

3.10.2 Z-RESET Switch Operation............................................................................................................ 71

3.10.3 Retraction and Refocusing of Objective ......................................................................................... 72

3.11 Objective Operation ...................................................................................................................................... 73

3.11.1 Phase Contrast Objectives............................................................................................................. 73

3.11.2 Cover Glass Thickness .................................................................................................................. 73

3.11.3 Objectives with Correction Ring ..................................................................................................... 74

3.11.4 Oil Immersion Objectives ............................................................................................................... 75

3.11.5 Water Immersion Objectives ..........................................................................................................77

3.12 Pillar Illuminator 100W Operation .................................................................................................................78

3.12.1 Condenser Refocusing Clamp ....................................................................................................... 78

3.12.2 Condenser Mount Rotation ............................................................................................................ 78

3.12.3 Pillar Inclination .............................................................................................................................. 79

3.12.4 Device Attachment Screw Holes ....................................................................................................79

3.13 Rectangular Mechanical Stage Operation..................................................................................................... 80

3.14 PFS (Perfect Focus System) Operation........................................................................................................ 81

3.14.1 PFS Overview ................................................................................................................................ 81

3.14.2 Starting and Stopping In-focus Observation with the PFS .............................................................. 84

3.14.3 Offset Adjustment........................................................................................................................... 86

3.14.4 Registration and Restoration of Offset ...........................................................................................87

3.14.5 If the Objective is Changed ............................................................................................................ 88

3.14.6 If the PFS Function Does Not Start ................................................................................................ 88

4. Assembly................................................................................................................................................ 89

5. Troubleshooting ...................................................................................................................................118

5.1 Image Viewing ............................................................................................................................................ 118

5.2 Operation .................................................................................................................................................... 120

5.3 Electrical System ........................................................................................................................................120

5.4 Perfect Focus System................................................................................................................................. 121

6. Daily Maintenance ............................................................................................................................... 123

6.1 Cleaning Optical Components ....................................................................................................................123

6.2 Cleaning the Microscope Body ...................................................................................................................123

6.3 Disinfecting the Microscope ........................................................................................................................ 123

6.4 Storage ....................................................................................................................................................... 123

6.5 Periodic Inspections (Paid Service) ............................................................................................................ 123

7. Specifications ...................................................................................................................................... 124

7.1 Microscope (Ti-E or Ti-E/B) with TI-DH Dia Pillar Illuminator 100W ............................................................ 124

7.2 Microscope (Ti-E or Ti-E/B) with TI-DS Dia Pillar Illuminator 30W .............................................................. 125

7.3 PFS Motorized Nosepiece and PFS Offset Controller ................................................................................126

7.4 AC Adapter ................................................................................................................................................. 128

7.5 Power Cord................................................................................................................................................. 128

7.5.1 Power Cord for the AC Adapter .................................................................................................... 128

7.5.2 Power Cord for the Power Supply ................................................................................................ 128

7.6 Safety Standards Compliance..................................................................................................................... 129

System Diagram........................................................................................................................................... 130

This chapter describes the name of each part of the product.

When using the product for the first time, refer to this chapter and check the name and the position of each part. Also refer to this chapter for names and positions of the controls whenever necessary.

• The components of the microscope can be selected to suit the application. However, the lamp, the dia pillar illuminator, and the power supply device must be used in specific combinations. Do not use these devices in an inappropriate combination. For combinations of the lamp, the dia pillar illuminator, and the power supply device, refer to page 55.

• For details on microscopy procedures, see Chapter 2, “Microscopy.” For details on the operation of each part, see Chapter 3, “Operation.”

• If the microscope has not been assembled yet, first refer to Chapter 4, “Assembly.”

• When using the TI-HUBC/A Hub Controller A with the Ti-E or Ti-E/B microscope body, motorized devices can be controlled with an external controller. For details, refer to the instruction manual provided with TI-HUBC/A Hub Controller A.

Part Names

Chapter 1 Part Names

(

1.1 Ti-E, Ti-E/B

The illustrations below show the Ti-E microscope body with the following accessories:

TI-DH Dia Pillar Illuminator 100W, TI-C Condenser Turret, D-LH/LC Precentered Lamphouse LC, 12V 100W halogen lamp, TI-PS100W Power Supply, TI-SR Rectangular Mechanical Stage, TI-T-B Eyepiece Base Unit, TI-TD Eyepiece Tube B, CFI 10x eyepieces, TI-ND6-PFS Perfect Focus Unit, objectives, etc.

Field diaphragm knob

Dia illumination lamphouse (Figure: D-LH/LC)

Filter sliders

both sides, 4 total)

Aperture diaphragm open/close leve

Condenser centering knob

Condenser turret (Figure: TI-C)

Eyepieces (both sides)

Eyepiece tube (Figure: TI-TD)

Eyepiece base unit

(Figure: TI-T-B)

Power supply device (Figure: TI-PS100W)

Front operation panel

Microscope base

(Figure: Ti-E)

Condenser lens

Condenser mount

Condenser focus knobs (both sides)

Dia pillar illuminator (Figure: TI-DH)

Objectives

Stage ring (concentric rin

Stage (Figure: TI-SR)

Nosepiece (Figure: TI-ND6-PFS)

OUT

DICHROIC MIRROR

Right side port *

Stage knob

Right connector panel

PFS

Coarse

Fine

BRIGHTNESS

FOCUS

DISPLAY

RESET

EYE

MEMORY

L100

PFS

R100

L80

1.5X

Epi Shutter

ExFine

FL Block

Refocus

Escape

Focus knob

Right operation panel

OFFSET

PFS

CONTROLLER

OFFSET

PFS

COARSE

FINE

DOWN

PFS Offset Controller

Figure 1-1 Ti-E

RESET

DISPLAY

RECALL

BRIGHTNESS

MEMORY

FOCUS

PFS

EYE

R100

L100

L80

Left operation panel

Left side port *

Focus knob

ExFine Fine Coarse

Obj.

OFF

6V30W

12V100W

MAX.

Figure 1-2 Ti-E (left view)

* Attach a protective cap on the side port when the side port is not in use.

Chapter 1 Part Names

1.2 Microscope Base

1.2.1 Microscope Base

To keep out extraneous light and dust, be sure to attach the provided caps to all ports not in use.

Nosepiece mount

Cove

Dia pillar illuminator mount (A carrying handle is attached for transportation.)

Eyepiece base unit mount

Status display panel

Front operation panel

Intermediate magnification

selector knob (1.5x ↔ 1x)

Left view

Epi illumination

field diaphragm

Filter turret mount

Right operation panel

slider mount

BRIGHTNESS

FOCUS

DISPLAY

EYE

MEMORY

L100

PFS

R100

L80

1.5X

Left side port

Coarse

Fine

ExFine

Z RESET

Epi Shutter

FL Block

Refocus

Escape

Figure 1-3 Microscope base

Focus knob

nalyzer slot (A dummy slider is attached for transportation.)

Connector panel (rear)

PFS

OFFSET

PIEZO

TERLOCK

Connector panel (right side)

Right side port

Bottom port (on the bottom, Ti-E/B only)

Focus knob

Coarse Fine ExFine

Obj.

OFF

6V30W

12V100W

MAX.MIN.

Left operation panel

Figure 1-4 Microscope base (left view)

1.2.2 Operation Panels

Front operation panel

Chapter 1 Part Names

1.2 Microscope Base

Status display panel (20x2)

DISPLAY switches

(display item selector)

BRIGHTNESS switch

(display brightness selector)

Optical path selector switches/indicators

• Ti-E : EYE, L100, R100, L80

• Ti-E/B : EYE, L100, R100, B100

Intermediate magnification selector dial

Left operation panel

Coarse/Fine/ExFine

switch/indicato

(focus knob resolution

selector/indicator)

Focus knob

FOCUS

EYE

L80

DISPLAY

ON RECALL

MEMORY

PFS

R100L100

RESETBRIGHTNESS

1.5X

(1.5X ↔ 1X）

Figure 1-5 Front operation panel

Coarse Fine ExFine

Obj.

OFF

6V30W

12V100W

Z-RESET switch (Z-axis position reset)

PFS control switches/indicators

• FOCUS indicator (PFS function status)

• PFS-ON switch (PFS ON/OFF selector/indicator)

• PFS-MEMORY switch (offset memory/indicator)

• PFS-RECALL switch (offset recall)

Obj. switch (objective selector)

Dia illumination lamp ON/OFF switch

Right operation panel

Epi Shutter switch

(epi illumination shutter operation)

FL Block switch

(filter block selector)

Refocus switch

(objective refocusing)

Escape switch

(objective retraction)

MAX.MIN.

Figure 1-6 Left operation panel

Coarse

Fine

ExFine

Epi Shutter

FL Block

Refocus

Escape

Figure 1-7 Right operation panel

Dia illumination lamp brightness control knob

Coarse/Fine/ExFine switch/indicator (focus knob resolution selector/indicator)

Focus knob

1.2.3 Connector Panels

Right connector panel

Chapter 1 Part Names

1.2 Microscope Base

OFFSET connecto

(for PFS offset controller)

INTERLOCK connecto

(for external laser controller)

Rear connector panel

Grooves for

motorized units wiring

(both sides)

Hub Controller A attachment

screw holes (x4)

POWER switch

MIC CONTROL connecto

(for Hub Controller A)

OFFSET

INTERLOCK

PFS

PIEZO

Figure 1-8 Right connector panel

TUV Rheinland

INSPECTION

OFF ON

POWER

MIC CONTROL

Gepruftes Medizinprodukt

Freiwillige Produktprufung

Product Safety

Approved medical device

EQUIPMENT 4N75 MODEL : Ti-E

This device complies with Part 15 of the FCC Rules.Operation is subject to the following two conditions: (1) this device may not cause harmful interference,and

this device must accept any interference received, including interference

(2)

that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numØrique de la classe A est conforme  la norme NMB-003 du Canada.

DC24V IN

USB

LAMP CTRL

5 3 1 0 0 1

JAPAN

Figure 1-9 Microscope base (rear view)

PFS connector (for PFS motorized nosepiece)

PIEZO connector (for PIEZO motorized stage)

Epi-fl illumination mount

Epi-fl illumination attachment screw holes (x4)

DC24V IN connector (for AC adapter)

LAMP CTRL connector (for dia illumination power supply)

USB connector (for PC)

Chapter 1 Part Names

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces

The following eyepiece base units, eyepiece tubes, and eyepieces can be mounted on the observation port of the microscope.

TI-T-B Eyepiece Base Unit

This is a basic type eyepiece tube base.

There is a dovetail (truncated cone) joint at the top to attach an eyepiece tube, which is used for visible observation.

If you don’t need visible observation, the mount can be left unused. In this case, attach a cover to the dovetail joint.

Eyepiece tube

mount

Microscope attachment

side (bottom)

Figure 1-10 TI-T-B Eyepiece Base Unit

Stage mount

Dovetail joint

Eyepiece tube clamp screw

TI-T-BS Eyepiece Base Unit (with Side Port)

This is an eyepiece tube base with a camera port.

Use the optical path selector knot at right to change the optical path between the eyepiece side and the side port side.

A direct C-mount adapter is attached to the side port

Eyepiece tube

mount

Stage mount

Dovetail joint

Eyepiece tube clamp screw

for camera connection.

(with C-mount adapter)

Side port

Microscope attachment

side (bottom)

Figure 1-11 TI-T-BS Eyepiece Base Unit

(with Side Port)

Optical path selector knob

TI-T-BPH Eyepiece Base Unit (for External Phase Contrast)

Eyepiece tube mount

Side port

(with C-mount adapter)

Turr et

Centering adjustment

screws (2 places)

Focus adjustment screw

Figure 1-12 TI-T-BPH Eyepiece Base Unit

(for External Phase Contrast)

Dovetail joint

Stage mount

Eyepiece tube clamp screw

Optical path

selector knob

Centering clamp screws (rear, 2 places)

Microscope attachment side (bottom)

This is an eyepiece tube base for external phase contrast microscopy.

A turret is provided at the front to attach external phase plates. Three positions, “A” to “C”, are available. Focus settings can be adjusted for three positions individually. Besides, phase plates can be centered with the screws at both side and clamped with the screw at the back.

A side port with a C-mount adapter is provided at left. Use the optical path selector knot at right to change the optical path between the eyepiece side and the side port side.

Use the side port for photomicroscopy of external phase contrast microscopy.

TI-TS Eyepiece Tube B

Chapter 1 Part Names

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces

Eyepiece

(both sides,

sold separately)

Binocular part

Figure 1-13 TI-TS Eyepiece Tube B

TI-TD Eyepiece Tube B

Eyepiece

(both sides,

sold separately)

Binocular part

Bertrand lens

in/out leve

Figure 1-14 TI-TD

Shutter open/close lever

Bertrand lens focus knob

Eyepiece Tube B

This is a basic type eyepiece tube.

This is an eyepiece tube with a manual shutter and a Bertrand lens.

The optical path for the binocular part can be blocked with the shutter for photomicrography.

Besides, the objective pupil can be observed when the Bertrand lens is placed in the optical path.

TI-TERG Ergonomic Eyepiece Tube

Eyepiece

(both sides,

sold separately)

Shutter

Binocular part

Bertrand lens

in/out leve

open/close lever

Bertrand lens focus knob

Figure 1-15 TI-TERG

Ergonomic Eyepiece Tube

This is an eyepiece tube with a tilt mechanism for user's physical attribute.

This eyepiece tube has a manual shutter and a Bertrand lens. The optical path for the binocular part can be blocked with the shutter for photomicrography. Besides, the objective pupil can be observed when the Bertrand lens is placed in the optical path.

Chapter 1 Part Names

1.4 PFS Motorized Nosepiece and PFS Offset Controller

The PFS Motorized Nosepiece (TI-ND6-PFS Perfect Focus Unit) is an integrated combination of a motorized sextuple DIC nosepiece and a perfect focus system (PFS). The offset can be controlled with the PFS Offset Controller.

PFS Motorized Nosepiece

Objective sockets (x6)

DIC prism ports (x6)

PFS Offset Controller

FINE/COARSE selector switch

4x and 10x objectives can be operated in FINE mode only. The FINE/COARSE selector switch will be disabled for these objectives.

Mounting screws

OUT

DICHROIC MIRROR

Figure 1-16 PFS Motorized Nosepiece

CONTROLLER

OFFSET

COARSE

FINE

PFS

DOWN

PFS

Interface cable (Connect to OFFSET connector on the Ti-E microscope body.)

Offset dial

Base plate (detachable, magnetic)

Figure 1-17 PFS Offset Controller

Optical component housing (Do not place items on the box. Do not apply excessive force.)

DICHROIC MIRROR - IN/OUT lever (dichroic mirror operation lever)

Main body connection cable (bottom)

* The offset controller body is magnetically attached to the base plate.

Use caution when lifting PFS Offset Controller, as the base plate may become detached and fall.

Chapter 1 Part Names

1.5 Stage

The following stages can be attached to the product.

TI-SR Rectangular Mechanical Stage

Stage ring (concentric ring)

Rack

Stage knob

• Y-axis direction

Figure 1-18 Rectangular Mechanical Stage

TI-SP Plain Stage

• X-axis direction

The specimen can be moved in the X and Y directions by operating the stage knob.

The rectangular mechanical stage comes with two stage clips for culture vessels, and the following two concentric rings.

25 mm bore

(with oiling notch)

40 mm bore

Stage ring (concentric ring)

Figure 1-19 Plain Stage

TI-SAM Attachable Mechanical Stage

ttachable

mechanical stage

Plain stage

Figure 1-20 Attachable Mechanical Stage

(on Plain Stage)

Stage knob

• Y-axis direction

• X-axis direction

Simple, fixed stage for easy operation of specimens.

The plain stage comes with the following two concentric rings.

25 mm bore

(with oiling notch)

This stage is attached to the plain stage when using

40 mm bore

specimen holders. The specimen can be moved in the X and Y directions by operating the stage knob.

Various specimen holders can be used by attaching the following adapters to the provided microplate.

• MA60 microplate holder

• MA60 Petri dish holder

• MA glass slide holder

• 35 Petri dish holder

Chapter 1 Part Names

1.6 Dia Pillar Illuminator

Ti series microscopes can be used with the following two types of dia pillar illuminators. The two dia pillar illuminators differ in lamp rating (12V 100W or 6V 30W) and support different microscopy methods. Select the dia pillar illuminator to suit your application.

1.6.1 TI-DH Dia Pillar Illuminator 100W

The TI-DH Dia Pillar Illuminator 100W is used with a separate lamphouse and condenser. The following illustration shows the TI-DH Dia Pillar Illuminator 100W with the D-LH/LC Precentered Lamphouse and the TI-C Condenser Turret (System Condenser) attached.

Filter sliders (both sides, 4 total)

Condenser mount

(rotatable)

Condenser mount

rotation clamp scre

Condense

centering knob

Field diaphragm knob

Condenser clamp scre

Condenser lens

TI-C Condenser Turret

(system condenser)

Figure 1-21 TI-DH Dia Pillar Illuminator 100W

(with lamphouse and system condenser)

1.6.2 TI-DS Dia Pillar Illuminator 30W

D-LH/LC Precentered Lamphouse LC (Requires TI-PS100W power supply)

Lamp cable

Condenser refocusing clamp

Condenser holder clamp screw

Condenser holder (detachable) Condenser focus knobs (both sides)

Pillar locking clamp screw

The TI-DS Dia Pillar Illuminator 30W has a built-in lamphouse, but requires a separate condenser. The following illustration shows the TI-DS Dia Pillar Illuminator 30W with the ELWD-S Condenser.

Filter sliders (3 total)

Dust-proof slide

Condenser mount

Condenser holde

ELWD-S Condense

DS 30W

30W

6V HALOGEN

Figure 1-22 TI-DS Dia Pillar Illuminator 30W

(with ELWD-S Condenser)

Lamphouse (6V 30W lamp sold separately) (Requires TE-PS30W or TE-PSE30 Power Supply A.)

Field diaphragm slider

Device attachment screw holes (M4, x8)

Chapter 1 Part Names

1.7 Condenser

1.7.1 TI-C Condenser Turret (System Condenser)

The TI-C Condenser Turret allows you to attach various optical elements to a turret and select them as necessary for different microscopy methods. This type of condenser is referred to as a “system condenser”.

System condenser (mounted on TI-DH Dia Pillar Illuminator 100W)

Condenser focus knob

Condenser centering knob

Aperture diaphragm

open/close leve

Condenser turret

Condenser lens

(4 types: ELWD, LWD,

HMC, and CLWD)

Figure 1-23 TI-C Condenser Turret

(mounted on TI-DH Dia Pillar Illuminator 100W)

System condenser (mounted on TI-DS Dia Pillar Illuminator 30W)

Condenser holde

Condenser focus knob

Condenser cassettes (Ph condenser cassettes have phase contrast ring centering screws on the sides.)

Condenser cassette clamp screws (both sides of cassette)

Condenser cassettes

Aperture diaphragm

open/close leve

Condenser turret

Condenser lens

(for HMC only)

Figure 1-24 TI-C Condenser Turret

Condenser cassette clamp screws (both sides of cassette)

(mounted on TI-DS Dia Pillar Illuminator 30W)

* The system condenser can be mounted on the TI-DS Dia Pillar Illuminator 30W for HMC observation only.

1.7.2 ELWD-S Condenser

(

)

Centering knob

Clamp screws

(both sides)

Condense

centering knobs

both sides

Turr et

Chapter 1 Part Names

1.7 Condenser

The ELWD-S Condenser supports bright-field microscopy and phase contrast microscopy. It can be used with both 100W and 30W dia pillar illuminators.

Condenser lens

Figure 1-25 ELWD-S Condenser

Chapter 1 Part Names

1.8 Power Supply

1.8.1 TI-PS100W Power Supply

The bottom of the power supply device becomes hot during use. Do not block the air vents on the side of the product.

Warning

POWER indicato

Brightness

control knob

POWER switch

Figure 1-26 TI-PS100W Power Supply

AC inlet

POWER switch

This is the power switch for the power supply. Press the “I” side of the switch to turn on the power supply and output DC power from the 12VDC output on the rear. Press the “O” side of the switch to turn off the power supply.

POWER indicator

Lit when the power supply is on.

Brightness control knob

When the EXTERNAL switch is turned off, the knob controls the brightness of the lamp by adjusting the voltage supplied from the 12VDC output connector.

AC inlet

This is the connector for connecting the power supply device to a wall outlet. Be sure to use the specified power cord for the connection.

EXTERNAL (external control) ON/OFF switch

Turn this switch on to use the brightness control knob on the microscope for output voltage control. When this switch is turned off, the brightness control knob on the front of the power supply becomes enabled, and the brightness control knob on the microscope becomes disabled.

12VDC output connecto

EXTERNAL (external control signal input)

EXTERNAL connector

EXTERNAL ON/OFF switch

connector

Connect the control cable to this connector and the LAMP CTRL connector on the rear of the microscope.

Pin Signal

External resistor terminal for output voltage adjustment

Output voltage ON/OFF switch (input)

GND (0V)

External voltage input for output voltage adjustment

EXTERNAL switch on/off detect signal (output)

GND (0V)

Output voltage monitor terminal (output)

Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.

12VDC output connector

This connector supplies power to the 12V 100W halogen lamp. Connect the lamp cable for the pillar illuminator.

Pin Signal

Output +

Output -

Not used

Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

Chapter 1 Part Names

1.8 Power Supply

1.8.2 TE-PS30W Power Supply A (for 100-120V)

TE-PSE30 Power Supply A (for 220-240V)

• Before turning on the power supply, check that the input voltage indicator matches the

• The bottom of the power supply device becomes hot during use. Do not block the air vents

Warning

power voltage in your area. If the voltages do not match, do not turn on the product, and contact Nikon. Use of the product under a wrong voltage may result in malfunction or fire.

on the side of the product.

Brightness

control knob

POWER

switch/indicato

Figure 1-27 TE-PS30W, TE-PSE30 Power Supply A

AC inlet

POWER switch/indicator

This is the power switch for the power supply. Press the “I” side of the switch to turn on the power supply and output DC power from the 6VDC output on the rear. The switch is lit when the power supply is on. Press the “O” side of the switch to turn off the power supply.

Brightness control knob

When the CTRL switch is turned off, this knob controls the brightness of the lamp by adjusting the voltage output from the 6VDC output connector.

AC inlet

This is the connector for connecting the power supply device to a wall outlet. Be sure to use the specified power cord for the connection.

CTRL (external control) ON/OFF switch

CTRL ON/OFF switch

CTRL (external control signal input) connector

6VDC output connector CTRL connector

Connect the control cable to this connector and the LAMP CTRL connector on the rear of the microscope.

Pin Signal

External resistor terminal for output voltage adjustment

Output voltage ON/OFF switch (input)

GND (0V)

Not used

Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.

6VDC output connector

This connector supplies power to the 6V 30W halogen lamp. Connect the lamp cable for the pillar illuminator.

Pin Signal

Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

Output -

Not used

Output +

1.9 AC Adapter

Chapter 1 Part Names

1.9 AC Adapter

Power to the microscope is supplied via an AC

C adapter

adapter.

The AC adapter can be used with 100 to 240 VAC at 50-60 Hz, and can be used with most wall outlets in the world.

DC output cable

Figure 1-28 AC adapter

C inlet

• Use the AC adapter included with the product.

• Use a power cord specified in Chapter 7, “Specifications”.

Microscopy

• Before using the product, thoroughly read the “Safety Precautions” at the beginning of this

• To use other equipment such as epi-fl attachment or differential interference contrast

When using the PFS Motorized Nosepiece, use the “Ti Control” setup software for Ti series to register the objective information from a PC. PFS Motorized Nosepiece will not function properly unless the objective information has been registered correctly. For details on using “Ti Control”, refer to the “Ti Control” instruction manual.

Warning

manual, and heed all warnings and cautions written therein.

attachment, refer to the respective manuals and heed all warnings and cautions written therein.

Caution

When using Ti-E or Ti-E/B with

TI-HUBC/A Hub Controller A attached on the back

Refer to the instruction manual provided with TI-HUBA/B Hub Controller A

The TI-HUBC/A Hub Controller A is used to control the motorized components.

When the TI-HUBC/A Hub Controller A is attached to the back of the microscope, operation procedures for the motorized devices will change. Refer to the instruction manual for the TI-HUBC/A Hub Controller A, and prepare and observe accordingly.

Note that the following two operations cannot be performed electrically even if the TI-HUBC/A Hub Controller A is attached:

• 6V 30W lamp operation: Use the dia illumination lamp ON/OFF switch.

• 6V 30W lamp voltage adjustment: Use the brightness control knob on the microscope body or

on the power supply device.

Chapter 2 Microscopy

2.1 Introduction to Microscopy

The Ti series microscopes are system microscopes that offer a high degree of flexibility in system building for various purposes. A wide range of options is available for various parts, including the main body, dia illuminator, and eyepiece tube. For this reason, the operation procedures will vary depending on the system configuration.

In this chapter, the following common configuration is used as an example to explain the microscopy procedures.

Sample configuration

Ti-E, PFS Motorized Nosepiece, TI-DH Dia Pillar Illuminator 100W, LWD Condenser, and TI-TD Eyepiece Tube B

Bright-field microscopy (see 2.2)

Phase contrast microscopy (without external phase contrast devices) (see 2.3)

External phase contrast microscopy (see 2.4)

In-focus observation with PFS (see 2.5)

• For the name and position of each part, refer to Chapter 1, “Part Names.”

• For details on operation methods, refer to Chapter 3, “Operation.”

• If the microscope has not been assembled yet, first refer to Chapter 4, “Assembly.”

• If the configuration of your microscope system differs from the examples used in this chapter, refer to the relevant sections of Chapter 3, “Operation.”

• If your microscope system has the epi-fl attachment or the differential interference contrast attachment, refer to the respective instruction manuals.

• If your microscope system is equipped with the TI-HUBC/A Hub Controller A, refer to the instruction manual provided with the hub controller.

+ 103 hidden pages

Nikon Eclipse Ti-E, Eclipse Ti-E/B Instructions Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Introduction

Safety Precautions

Warning and Caution Symbols in this Manual

Symbols on the Product

LED Safety

Warning

Caution

Notes on Handling the Product

Contents

1.1 Ti-E, Ti-E/B

1.2 Microscope Base

1.2.1 Microscope Base

1.2.2 Operation Panels

1.2.3 Connector Panels

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces

1.4 PFS Motorized Nosepiece and PFS Offset Controller

1.5 Stage

1.6 Dia Pillar Illuminator

1.6.1 TI-DH Dia Pillar Illuminator 100W

1.6.2 TI-DS Dia Pillar Illuminator 30W

1.7 Condenser

1.7.1 TI-C Condenser Turret (System Condenser)

1.7.2 ELWD-S Condenser

1.8 Power Supply

1.8.1 TI-PS100W Power Supply

1.9 AC Adapter

2.1 Introduction to Microscopy