Nikon Eclipse Ni-U Instruction Manual

M570 E 11.8.NF.1 (2/2)

Assembly

Troubleshooting

Maintenance and

Upright Microscope

Instruction Manual

Assembly/Maintenance

Storage

Specifications

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon ECLIPSE Ni-U microscope. To ensure correct usage, read this manual carefully before operating this product.

● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

● The contents of this manual are subject to change without notice.

● The equipment described in this manual may differ from the actual product in its appearance.

● Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If

you note any points that are unclear or incorrect, please contact your nearest Nikon representative.

● Some of the equipment described in this manual may not be included in the set you have purchased.

● If you intend to use any other equipment with this product, read the manual for that equipment too.

● If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may

be impaired.

● Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your opinion.

Introduction

Contents of the Manual

The instruction manual for ECLIPSE Ni-U is provided as two volumes.

 Operation

Safety Precautions Components Microscopy Procedures

Operation Flowchart Bright/Dark-field Microscopy Epi-fluorescence Microscopy Differential Interference Contrast Microscopy Phase Contrast Microscopy Motorized Bright-field Microscopy Motorized Epi-fluorescence Microscopy

Individual Operations

Symbols Used in This Manual

The following symbols are used in this manual.

 Symbols for Safety

WARNING

CAUTION

 Other Symbols

Highlights important information that should be noted for safety. Read “Safety Precautions” for details.

 Assembly/Maintenance (This manual)

Assembly Troubleshooting Maintenance and Storage Specifications

Before reading the “Assembly/Maintenance” manual, read the “Safety Precautions” in the “Operation” manual.

Indicates information you should note or comply with to prevent defects or malfunction of this product.

Indicates information you should be aware of in using this product, as well as other useful information.

Summary of Contents

Introduction

Contents of the Manual Symbols Used in This Manual

(See the next page for the detailed contents.)

Assembly

Troubleshooting

Maintenance and Storage

Assembly

Troubleshooting

and Stora

Specifications

Specifications Maintenance

iii

Contents

Introduction .........................................................................................................................i

Contents of the Manual ....................................................................................................ii

Symbols Used in This Manual..........................................................................................ii

Summary of Contents ....................................................................................................... iii

Assembly.................................................................................................. 1

1 ECLIPSE Ni-U System Configuration .......................................................................2

2 Components List ....................................................................................................... 3

3 Assembly Method......................................................................................................4

Introduction ...................................................................................................................... 4

[1] Check the input voltage...................................................................................... 4

[2] Attach the DIA motorized shutter (optional). ...................................................... 5

[3] Attach the lamp. ................................................................................................. 5

[4] Attach the lamphouse for dia-illumination. ......................................................... 5

[5] Attach the standard arm/contact arm................................................................. 6

[6] Attach the epi-fluorescence cube turret and epi-fluorescence attachment

(required for epi-fluorescence microscopy)........................................................ 7

■ Attaching a filter cube ............................................................................... 8

■ Replacing excitation and barrier filters ..................................................... 9

■ Attaching a light shielding plate ................................................................ 9

[7] Attach the quadrocular tilting tube and DSC zooming port

for quadrocular tube. ........................................................................................ 10

[8] Attach the EPI motorized shutter (optional). .................................................... 10

[9] Attach eyepieces. ..............................................................................................11

[10] Attach the substage...........................................................................................11

[11] Attach the condenser holder. ........................................................................... 12

[12] Attach the stage. .............................................................................................. 12

[12.1] For standard stage .......................................................................... 12

[12.2] For rotatable ceramic coated stage................................................. 12

[13] Attach the condenser........................................................................................ 13

■ Attaching the optical module to the universal condenser....................... 13

■ Attaching the DIC module to DIC condenser oil..................................... 17

[14] Attach the nosepiece........................................................................................ 19

[15] Attach the objective. ......................................................................................... 19

[16] Attach the DIC rotation polarizer unit (required for differential interference

contrast microscopy). ....................................................................................... 19

[17] Attach the eyelevel riser (optional)................................................................... 20

[18] Attach a camera and connect the DS camera control unit (optional). ............. 20

[18.1] When attaching to the DSC port for the ergonomic binocular tube 20

[18.2] When attaching to the DSC zooming port for quadrocular tube ..... 21

[18.3] When attaching to the quadrocular tilting tube................................ 21

[18.4] When attaching a camera to the trinocular tube ............................. 21

[19] Replace the ND filter (optional)........................................................................ 22

[20] Connect the motorized device cable................................................................ 22

[21] Connect the power cord. .................................................................................. 24

Troubleshooting..................................................................................... 25

1 Optical System and Operation ................................................................................26

1.1 General............................................................................................................. 26

1.2 Epi-fluorescence Microscopy ........................................................................... 30

1.3 Differential Interference Contrast Microscopy.................................................. 31

Contents

1.4 Phase Contrast Microscopy ............................................................................. 32

2 Electrical Requirements ..........................................................................................33

2.1 General............................................................................................................. 33

2.2 Epi-fluorescence Microscopy ........................................................................... 34

Maintenance and Storage ..................................................................... 35

1 Replacing the Lamp ................................................................................................36

2 Cleaning ..................................................................................................................37

2.1 Cleaning Lenses............................................................................................... 37

2.2 Cleaning Parts Other than the Lens................................................................. 37

2.3 Cleaning Immersion Oil.................................................................................... 38

2.4 Decontaminating the Product........................................................................... 38

3 Transportation .........................................................................................................38

4 Storage....................................................................................................................38

5 Regular Inspections (Charged) ...............................................................................38

Specifications ........................................................................................ 39

1 Microscopy (Principles) ...........................................................................................40

2 Performance Properties .......................................................................................... 40

3 Physical Properties .................................................................................................43

Contents

Chapter

Assembly

This chapter contains an Ni-U system configuration diagram, a list of its components, and explains how to assemble the system.

Read appropriate notes such as CAUTION “9 Cautions on assembling and installing the product” at the beginning of the separately provided instruction manual “Operation” or “4 Installation location” in “Notes on Handling the Product” in the same manual before you work on assembling.

Assembly

■ Assembly tools provided with the microscope

• Hex driver x3: 2 mm across flats x3

• Hex wrench x2: 3 and 4 mm across flats

Chapter 1 Assembly

Assembly

Centering telescope

Binocular tube

Eyelevel riser

Analyzer unit

ECLIPSE Ni-U System Configuration

ENG mount camera

C mount camera

Photomicrography device

CFI eyepiece

TV adapter

Trinocular tube

DSC port for ergonomic

binocular tube

Ergonomic tube

To control box B

Contact arm Standard arm

Various adapters

Tube adapter for quadrocular tube

Quadrocular tilting tube

Filter cube

Epi-fluorescence

cube turret*2

FL/DIC

analyzer

DS camera head

DSC zooming port

Epi-fluorescence

attachment

Excitation

balancer

Motorized shutter

D-FB

Camera cable to DS-U3 or DS-L3

Motorized shutter cable to control box B

Motorized shutte

HG adapter

adapter

HG fibe

Analyzer

slider

Lambda plate

DIC slider

DIC

nosepiece*1

Nosepiece

with

analyzer slot

Nosepiece

CFI objective

Ni-U main body

Substage fo

holder

rotatable ceramic

coated stage

Substage

Polarizer

unit

Control

box B

EPI FL INTSL

DIA

SHUTTER OPEN

12481632

INTSL ND

OBJ FL

CONTROL

NI-SRCP

MADE IN JAPAN

Simple remote

control pad

Rotatable ceramic

coated stage

Stage

Achr-Apl N.A=1.4

0.20.40.60.81.01.21.4

Condenser

*1: A contact arm is required to use a motorized/intelligent nosepiece.

Condenser

*2: A contact arm is required to use a motorized/intelligent epi-fluorescence cube turret.

*3: DS-U3 DS camera control unit can not be connected via USB.

RS-232C cable to control box B for motorized operation

Motorized shutter cable to control box B

Motorized

shutter

DS-L3/

DS-U3 Camera

Control Unit

Camera trigger cable to

Ni-U main body or

control box B

USB cable to

control box B*3

Mercury lamp

Motorized

shutter

adapter

HG precentered

fiber illuminator

To Ni-U

main body

Lamphouse

Chapter 1 Assembly

The ECLIPSE Ni-U components are shown in the table below. Depending on when you purchased the ECLIPSE Ni-U, there may be devices not yet available for use and devices not listed that are already available for use. Contact your nearest Nikon representative for details.

Device Device Name Model Remarks Microscope main body

Illuminator for dia-illumination

Arm

Tube

DSC port

Eyepiece

Nosepiece

Objective CFI objective

Substage

Stage

Condenser holder Condenser holder NI-CH

Condenser

Epi-fluorescence cube turret

Epi-fluorescence attachment Mercury lamp illuminator

Analyzer unit/ Slider

Polarizer unit

Shutter

Controller

Eyelevel riser Eyelevel riser C-ER Variable intermediate magnification unit

DSC retention support

Tube adapter for quadrocular tube

TV adapter TV adapter

Components List

ECLIPSE Ni-U (12V 1 00W 100-240V power sup ply for illumination integrated) Precentered lampho use (12V 100W) NI-LH

Halogen lamp (100W)

Standard arm NI-SAM Combine with a manual model such as manual nosepiece Contact arm NIU-CAM Combine with a motorize d models such as intelligent /motorized nosepiec e Binocular tube C-TB C-TBM mildew-proof type also available Trinocular eyepiece tube F C-TF C-TFM mildew-proof type also available Trinocular eyepiece tube T C-TT Ergonomic tube C-TE2 Quadrocular tilting tube NI-TT DSC port for ergonomic binocular tube C-TEP2 DSC zooming port for quadrocular tube NI-RPZ

CFI eyepiece

Centering telescope C-CT Universal quintuple nosepiece L-NU5 BD quintuple nosep iece L-NBD5 Sextuple nosepiece C-N Sextuple nosepiece with analyzer slot C-NA Sextuple DIC nosepiec e D -ND6 Intelligent DIC sextuple nosepiece NI-ND6-I Intelligent Septuple nosepiece NI-N7-I Motorized DIC sextuple nosepiece NI-ND6-E Motorized septuple nosepiece NI-N7-E

Substage NI-SS Rotatable ceramic coated stage can not be attached Substage for rotatable ceramic coated stage NI-SSR Right handle stage with 2S holder C-SR2S Left handle stage with 2S hold er C-SL2S Right handle ceramic coated stage with 1S holder C-CSR1S Left handle ceramic coated stage with 1S holder C-CSL1S NI-U right handle rotatable ceramic coated stage with holder NI-U left handle rotatable ceramic coated stage with holder

Dark field condenser (Oil) and (Dry) C-C aplanatic condenser C-C achromat conden ser No.9 C-C abbe condenser No.9 X LWD condenser C-C slide achro conde nser 2-100x C-C achromat swing-o ut condenser 1-100x C-C phase turret condenser Cannot be used when rotatable ceramic coated stage is attached. DIC condenser (Oil) D-CUO Used with OIL DIC module

Universal condenser (Dry) NI-CUD

Cube turret NI-FLT6 Intelligent epi-f luorescence cube t urret NI-FLT6-I Motorized epi-flu orescence cube turret NI-FLT6-E

Epi-fluorescence attachment NI-FLEI D-FB excitation balancer attachable.

HG precentered fiber illuminator C-HGFI Motorized HG precentered fiber illuminator C-HGFIE

Analyzer slider for DI C D-DA

Analyzer tube for simple polarization C-ISA Combined with C-SP polarizer unit. Analyzer tube for first -order red compensation C-IA Combined with C-TP polar izer unit. Analyzer slider for simple polarization D-SA Analyzer slider for first-order red compensation C-AS DIC slider D-C Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece. Lambda plate D-LP Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece. DIC rotatable polarizer unit D-DP Polarizer unit for simple polarization C-SP Polarizer unit for first-order red compensation C-TP

Motorized shutter NI-SH-E

Motorized shutter adapter for upright epi-fl NI-SHAEP-U Motorized shutter adapter for dia-illumination NI-SHADI Control box B NI-CTLB Simple remote control pad NI-SRCP

DS-L3/DS-U3 camera control unit

Variable intermediate magnif ication unit Y-IM

DSC support column NI-RPS

DSC adapter A and B

Tube adapter for quadrocular tube C-TAQ

ECLIPSE Ni-U

PHILIPS 7724 OSRAM HLX 64623

CFI CFI UW

CFI LU Plan Fluor Epi LU Plan Fluor BD

NIU-CSRR2

NIU-CSLR2

DS-L3 DS-U3

NI-RPSAA NI-RPSAB

Y-T V Y-T V5 5

Connected to Nikon DS camera head or other C mount camera. Nikon DS camera head is controlled from DS-L3/DS-U3.

LU Plan Fluor Epi/BD can be attached to the L-NU5 or L-NBD5 nosepiece. LU nosepiece adapter is required to attach a non-BD obje ctive to L-NBD5.

Supports DRY DIC module, PH module, dark field module, NI-CALN1 2-4x auxiliary lens, D-C 2-4x auxiliary lens

A filter cube and NI-FA FL/DIC analyzer attachable.

Can be attached to C-NA, D-ND6, NI-ND6-I, NI-N7-I, NI-ND6-E, or NI-N7-E nosepiece.

For connection, NI-S HCL motorized shutter cable is req uired and is connected to t he control box B.

DS-L3 is connected to the control box B via USB cable DS-L3/DS-U3 is con nected to the Ni-U m ain body or the contr ol box B via C-CTC camera trigger cable L3/U3 DS camera I/F cable is used to connect to the camera

Can be attached to the quadrocular tilting tube. Attach a C mount camera, ENG mount camera or a photomicrography device via various adapters. Y-TV can be attached to C-TF or C-TT trinocular tube. C onnect a C mount camera, ENG mount camera, or photomicroscopy device with an adapter. Y-TV55 can be attached to C-TF or C-TT trinocular tube. Connect a C mount cam era with C mount adapter 0.55x.

Assembly

Chapter 1 Assembly

Assembly

Assembly Method

Introduction

Ni-U is a manual microscope. Basically, a manual model is attached for each device consisting the microscope. However, for nosepiece, epi-fluorescence cube turret, and HG precentered fiber illuminator, motorized models can be attached. In this case, contact arm, control box B, and simple remote control pad are required. In addition, in order to configure information for motorized devices, DS-L3 DS camera control unit must be connected. Motorized devices on Ni-U can be controlled from DS-L3.

Note that when using epi-fluorescence cube turrets by layering, motorized/intelligent epi-fluorescence cube turret can only be used in 1st layer.

Assemble the device according to the following procedure.

Precautions for connecting cables

Cable connections are required for some motorized devices.

Be sure to turn off the power to the microscope and peripheral devices before starting cable connection.

Cable connections are described in each assembly step. Nikon recommends connecting cables at the end of the assembly. See “20 Connect the motorized device cable.” for the connector connections.

Information setting regarding motorized models

Set information of the motorized devices or optical element attached on the microscope setup menu for the DS-L3 DS Camera Control Unit. (See Chapter 3 “19 Operations on DS-L3” - “19.1 Setting Up the Microscope.)

Check the input voltage.

Check the input voltage indicated on the back of the microscope. Use the microscope only if the indicated input voltage matches the power supply voltage for the area in which the microscope will be used.

WARNING

Input voltage

If the indicated voltage and the supplied voltage differ, do not attempt to use the microscope. Contact your nearest Nikon representative.

Unlocking the elevating section

Tool: flathead screwdriver

After you confirm that the input voltage is correct, loosen and remove the tightening screw at the front of the elevating section.

label

Tightening screw

MODEL ECLIPSE Ni-U

NIKON CORPORATION

100–240V~

920001

TOKYO, JAPAN

1.7A MADE IN JAPAN

50/60Hz

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

INSPECTION EQUIPMENT

4N75

LAMP

DC12V 100W

Checking the input voltage

(Microscope rear view)

LAMP CTRL

(BOX B)

DSC

NCB

POWER

OUT

Unlocking the elevating section

Chapter 1 Assembly

Attach the DIA motorized shutter (optional).

The motorized shutter is attached by Nikon. Contact your nearest Nikon representative when the DIA motorized shutter needs to be attached or removed.

Attach the lamp.

Tool: Hex wrench (3 mm across flats)

Lamp handling precautions

Avoid touching the glass surface of the lamp with your bare hands.

(1) Loosen the lamphouse cover fixing screw and lift up

Fixing screw

the cover to remove.

(2) Insert the lamp clamp lever to open the socket pin

hole. Attach the lamp while holding down the lever. Put the lamp clamp lever back to its original position.

Designated lamp: PHILIPS7724

or OSRAM HLX64623

(3) Reattach the cover back to its original position and

tighten the lamphouse cover fixing screw.

Insert the hex wrench and

loosen the fixing screw.

Lamphouse cover

Removing the lamphouse cover

Lamp

Assembly

Lamphouse fixing screw

Attach the lamphouse for dia-illumination.

Tool: Hex driver (2 mm across flats)

(1) Insert the lamphouse at the rear of the microscope

and tighten the fixing screws.

(2) Connect the dia-illumination lamphouse cable to the

LAMP connector on the rear of the main body.

Attaching the lamp

Lamphouse fixing screw

MODEL ECLIPSE Ni-U

920001

100–240V~

MADE IN JAPAN

1.7A

NIKON CORPORATION

50/60Hz

LAMP

DC12V 100W

TOKYO, JAPAN

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

DSC

INSPECTION EQUIPMENT

4N75

LAMP CTRL

)

(

BOX B

Lamp clamp lever

-High Temperature-

CAUTION!

Do not touch the lamphouse while the lamp is lit.

1. The surface of the lamphouse becomes hot when the lamp is on. Turn off the power and allow the lamp and lamohouse

2. to cool enough before replacing the lamp. Wait for at least 30 minutes after turning off the lamp.

HALOGEN 12V100W

Use 12V100W HALOGEN lamp only.

NI-LH

952001

JAPAN

Securing the dia-illumination lamphouse

Assembly

Chapter 1 Assembly

Attach the standard arm/contact arm.

The attachment procedure is the same for standard arm

and contact arm. To use the motorized accessories, attach the contact arm. The contact arm has a contact. When attaching a motorized device on the arm, remove the contact cover beforehand.

Tool: Hex wrench (4 mm across flats)

(1) Place the arm while aligning it with the two

positioning pins on the main body and tighten the four fixing screws.

(2) When using contact arm, connect the cable from the

CONTACT ARM1 connector to the CONTACT ARM1 connector on the control box B.

(3) Connect the cable from the CONTACT ARM2

connector to the CONTACT ARM2 connector on the control box B.

rm fixing screw

(Standard arm)

Contact cover

Positioning pin

(Contact arm)

Attaching the arm

Chapter 1 Assembly

Attach the epi-fluorescence cube turret and epi-fluorescence attachment (required for

epi-fluorescence microscopy).

Tool: Hex driver (2 mm across flats)

Hex wrench (3 mm across flats)

First, connect the epi-fluorescence cube turret and epi-fluorescence attachment and then attach them on the microscope arm.

(1) Insert the positioning pin of the epi-fluorescence

attachment while aligning it with the positioning hole on the epi-fluorescence cube turret side, and then tighten the connection fixing screws (x2) on the epi-fluorescence cube turret with a hex driver.

(2) When using a motorized or intelligent

epi-fluorescence cube turret, remove the contact cover on the contact arm beforehand. (Contact cover is not removed for manual epi-fluorescence cube turret.)

(3) Loosen the fixing screw on the front of the arm using

a hex driver so that the tip of the screw does not protrude into the connecting section.

(4) Align the round dovetail and convex contact on the

bottom of the epi-fluorescence cube turret with the round dovetail and concave contact of the arm and slide the entire mounted attachment to the rear. (There is no concave contact in manual epi-fluorescence cube turret.)

(5) Tighten the fixing screw loosened in step (3).

(6) Tighten the four fixing screws on top of the

epi-fluorescence attachment.

(7) Attach the HG adapter to the bayonet mount at the

rear of the epi-fluorescence attachment and connect the HG precentered fiber illuminator. (See the instruction manual provided with the HG precentered fiber illuminator.)

Epi-fluorescence cube turret

Positioning hole

Epi-fluorescence attachment

F. STOP

A. STOP

EX. ADJ

Connection fixing screw

Connecting epi-fluorescence cube turret

and epi-fluorescence attachment

A. STOP

F. STOP

EX. ADJ

Fixing screw

Attaching and fixing epi-fluorescence cube turret

and epi-fluorescence attachment set

Bayonet mount

HG adapter

F. STOP

A. STOP

EX. ADJ

Assembly

Epi-fluorescence attachment fixing screw

MODEL ECLIPSE Ni-U

920001

100–240V~

MADE IN JAPAN

1.7A

NIKON CORPORATION

50/60Hz

TOKYO, JAPAN

INSPECTION

Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

EQUIPMENT

4N75

Attaching the HG adapter

HG fiber

Assembly

Chapter 1 Assembly

■ Attaching a filter cube

Precautions for attaching and removing the cube

• Be sure to check that the light source is turned off before attaching or removing the cube.

• Be sure to check that the power switch for microscope main body is turned off and then attach the cube by

rotating the cube switchover turret. Also for the motorized epi-fluorescence cube turret, attach it by manually rotating the inside turret.

(1) Pull out the filter cube replacement cover on the

front of the epi-fluorescence cube turret to remove it.

(2) Attach the filter cube to the slot.

(3) Insert the filter cube nameplate into the slot cover

window.

ND4

ND8

Insert into the same address window as the address shown inside the slot.

ND16

(4) Turn the cube switchover turret to attach the filter

cubes in the remaining slots and also insert the nameplates.

For bright-field microscopy

For bright-field microscopy, be sure to make the address 1 empty.

For motorized/intelligent epi-fluorescence cube turret, only address 1 can be set to [OPEN].

(5) Restore the slot cover back to its original position.

UV-2A

EX 330-380 DM 400 BA 420

Attaching a filter cube

Filter cube

ddress

indication

Filter cube replacement slot cover

Cube switchover turret

ddress

indication

Nameplate window

+ 36 hidden pages

Nikon Eclipse Ni-U Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Introduction

Contents of the Manual

Symbols Used in This Manual

Summary of Contents

Contents

ECLIPSE Ni-U System Configuration

Components List

Assembly Method

Introduction

■ Attaching a filter cube