Nikon E200 User Manual

En

411.4 (Eyepoint height when PD is 64mm.)

191.3

315

407.4 (Minimum microscope height)

414.6 (When PD is 64mm.)

105

188.5

247.7

206.4

226.8

101.5

381.7 (When PD is 64mm.)

Unit: mm

Biological Microscope

Dimensional Diagram

Optical system CFI60 (infinity optical system)

Parfocal distance: 60mm

Magnification 40–1500X for observation

8–500X for 35mm photomicrography

Eyepiece tube E2-TB Binocular tube, E2-TF Trinocular tube

Siedentopf type (Inclination: 30°, Interpupillary
distance: 47-75 mm, 360° rotatable)
C-TE Ergonomic Binocular Tube (inclination: 10­30º, extension: 40mm)

Eyepiece CFI E 10X (F.O.V.: 20mm), CFI E 15X (F.O.V.: 12mm)
Photo lens PLI projection lens: 2X, 2.5X, 4X, 5X
Nosepiece Quadruple nosepiece, reversed type
Coarse/fine Fine: 0.2mm per rotation, Coarse: 37.7mm per

focusing rotation, Minimum reading: 2 microns on left-side

fine control knob, Coarse motion torque
adjustable, Refocusing system incorporated in
stage, Stage handle and focusing knob are at equal
distance from the operator

Stage Rectangular 216 x 150 mm surface stage mounted

on the main body. Cross travel 78 x 54 mm using
low-positioned X/Y coaxial control knob

Objectives CFI E Plan Achromat 4X N.A. 0.10 (W.D. 30mm)

CFI E Plan Achromat 10X N.A. 0.25 (W.D. 0.7mm)

CFI E Plan Achromat 40X N.A. 0.65 (W.D.

0.65mm)
CFI E Plan Achromat 100X Oil N.A. 1.25 (W.D.

0.23mm)
As an option, CFI Achromat DL and other
higher-grade CFI60 objectives can be used

Condenser E2 Abbe condenser N.A. 1.25; leaf-type

aperture diaphragm with position guide
markings for respective CFI E Plan objectives
Optional condensers:
E2 Phase condenser N.A. 1.25; leaf-type
aperture diaphragm with position guide
markings for respective CFI Achromat DL
objectives
For microscope without field diaphragm:
C-C Achromat Condenser
Achromat Swing-out Condenser
Darkfield Condenser, dry type
C-C Phase Contrast Condenser

Illumination system 6V-20W halogen bulb, 6V-30W halogen bulb
Intermediate E200 epi-fluorescence attachment, Teaching

attachment

* head (face-to-face and side-by-side types),

Drawing tube, Eye level riser

*Maximum intermediate space 50mm

E200 Specifications

Printed in Japan (0810-05)T Code No. 2CE-MTDH-8

This brochure is printed on recycled paper made from 40% used material.

Specifications and equipment are subject to change without any notice or obligation
on the part of the manufacturer. October 2008 ©2000-08 NIKON CORPORATION

WARNING

TO ENSURE CORRECT USAGE, READ THE CORRESPONDING
MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

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phone: [phone]; +1-800-52-NIKON (within the U.S.A. only)
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The first microscope in its class to feature CFI60 infinity optics,

plus an anti-mold design to en sure years of high-quality use

F

or the first time, the CFI60 optical system—

infinity optics of the world's highest level—has

been incorporated into a microscope of this class.

The result is stunningly sharp and clear images at

any magnification. Of course, the Eclipse E200

maintains the same operational ease and rigidity

common to all Nikon microscopes and is compatible

with many accessories for higher-grade Eclipse series

microscopes.

Is mold a problem? Not any more.

By using anti-mold paint and sealing anti-mold agent

inside the microscope at critical places, the Eclipse

E200 is designed for use even in hot and humid

environments.

Moreover, this instrument packs all of these features

into a contemporary design that blends in with the

clean interiors of your hospital and examination

rooms.

Best of all, the Eclipse E200 is affordable. Once you

use it, you'll realize what an outstanding performer it

is, despite its modest price.

Eclipse E200-F
(model with field diaphragm)

Eclipse E200

Superb infinity optics, plus ergonomic touches

The CFI60 optical system
combines Nikon's
renowned CF optical
design with infinity
optics to overcome the
limitations of the
traditional infinity

design. CFI60 optics
provide longer working distances and higher N.A.'s.
These new optics deliver startlingly clear images at
any magnification because chromatic aberrations and
curvature of field are both corrected over the entire
field of view when the field number is 20mm. Nikon
developed new dedicated CFI E Plan Achromat
objectives exclusively for the E200. Also, you can use
other higher-grade objectives available for the Eclipse
series whenever your laboratory situation calls for it.

Anti-mold design

Mold is a formidable enemy of microscopes. Before you know it, it can begin
growing on the interior optical surfaces of the microscope and ruin
performance. Using anti-mold paint, plus anti-mold agent sealed at critical
places inside the microscope, the Eclipse E200 is designed to resist mold
growth. In tests, an anti-mold treated unit was able to resist the growth of
mold for three consecutive years at an average temperature of 30° C (86° F)
and 80% humidity.

Ergonomic design
equals comfortable
operation

Comfort is ensured allowing long
hours of use, thanks to Nikon’s
thoughtful ergonomic design. This is
the same design incorporated into
Nikon’s other laboratory and research-grade Eclipse series
microscopes.
For example, the focus knob and the stage handle are located
equidistant from the operator, permitting one-handed operation in a
natural posture without twisting the shoulders. Because these controls
are low positioned, you can operate the microscope while resting your
arms comfortably on the desk. Moreover, the low-profile stage makes
exchange of specimen slides easy, while the low inclination angle
eyepiece tube provides comfortable viewing.

Ergonomic binocular tube

With this option, users can adjust not only the eyepiece
tube tilt angle, but the eyepiece length to suit their build,
eliminating discomfort and strain during long hours of
observation.

* Use of this accessory in combination with other equipment may

produce darker images around the periphery.

* Must be attached directly to the main body.

4 5

One-piece construction from arm to base, a stage design
where its up/down mechanism is located in the base, plus
a wide footprint of 188.5mm across the back all provide

greater rigidity and resistance to vibrations, contributing to
superior images.

optical system

Improved

Eye-level riser

Up to two eye-level risers can be mounted to raise the height of the eyepoint—25mm each
for a total of 50mm.

Robust, vibration resistant construction

Comparison of the positions of the stage handle and
focus control knob

View angle is adjustable by 10º to 30º.

Eyepiece length can be extended
by up to 40mm.

E200 configured with an
ergonomic binocular tube

Anti-mold

paint and agent

E200 Conventional models

Design ideas that ensure superb opti cal quality and increased ease of use

6 7

Revolving nosepiece

A reversed-type nosepiece creates more space at the front of the
stage, making handling of specimen slides fast and easy.
In addition, the CFI60 optical design eliminates extra optical
elements in the nosepiece for enhanced image sharpness. Another
advantage of CFI60 objectives is that their increased objective
lengths and longer working distances provide more working space
around the nosepiece.

Refocusing stage

Nikon has created a unique innovation. The Refocusing Stage
eliminates the need to refocus the image manually, making
specimen handling safer and easier. In this unique design, the
stage can be instantly dropped by pushing it down to exchange
specimens or oil the slide, then returns to the original position as
soon as the hand is removed. The wide stage surface can
accommodate two slide glasses at the same time.
In addition, this stage has an array of features including:
– Increased resistance to vibrations due to the design of the in-

base focus mechanism.

– Low-profile design that creates more space around the objective

for increased freedom in specimen handling and easier
operation.

– A belt-drive mechanism to eliminate the projection of the rack at

the edge of the stage for better ergonomics and smoother

movement.
– Removable specimen holder for fast hand scanning of slides.
– Improved XY cross travel, providing the comfort and feel similar

to Nikon’s higher grade Eclipse series microscopes.

Upper limit stopper

When using short-working-distance objectives such as 40X or greater, you
can set the upper limit of the stage movement, so that the objective doesn’t
hit the specimen slide, protecting both from damage. Thanks to this feature,
even novices or operators who need to change slides often can perform
their job easily and quickly. The limit height can be set in two levels using a
stopper bolt—either at the standard position or 2mm lower. This feature is
very useful except when extraordinarily thick specimens are used.

New

New

Eyepiece tube

The Siedentopf-type
eyepiece tube is inclined at
30 degrees to ensure
comfortable viewing in a
natural posture. Designed
for use by operators with
different builds, this
eyepiece tube has a narrow
minimum interpupillary
distance of 47mm, while
the eyepoint height can be
raised 34mm when
interpupillary distance is
64mm by simply swinging
the front part of the
eyepiece tube up 180˚. For
extremely tall users, eye­level risers are available to
customize the microscope.

Eyepieces

The E200’s new eyepieces feature a wider field of view for a
microscope of this class and are available in 10X (F.O.V. 20) and 15X
(F.O.V. 12) types. These eyepieces also feature built-in diopter
adjustment that allows the operator to adjust diopters separately for
the right and left easily. In addition, these eyepieces accept
measuring reticles that will always be in sharp focus with the
specimen. Moreover, they can be locked, preventing theft and
eliminating the possibility of damage during transit.

CFI60 objectives

Nikon’s exclusive CFI60 objectives provide numerous benefits: longer
working distances, high numerical apertures, flat images over the entire
field of view with virtually no curvature of field when the field number is
20mm. To match your laboratory requirements, the E200 provides a wide
selection of objectives to choose from. These include the new CFI E Plan
Achromat objectives developed for the E200 or other higher-grade
Eclipse series objectives.

Improved

CFI E Plan Achromat objectives

Low position High position

E2-TB binocular tube E2-TF trinocular tube

* Eyepiece lens is optional.

F.O.V. 18 (conventional model)F.O.V. 20 (E200)

Wide stage
surface with no
rack sticking out
of the stage

2mm

Cover glass

Slide glass

Upper limit (High)

Upper limit (Low)

Stage surface

A wealth of accessories to broaden
the range of microscopic applications

8 9

Condenser

Although the stage is low-positioned for comfort, there is ample space
around the condenser for easy access. The condenser also features an
aperture diaphragm that comes complete with position guide markings
for respective E Plan objectives to make operation quick and easy.

Replacing the lamp is safe and easy

Turning the microscope upside down to replace the lamp is no longer
necessary. Simply open the lens unit cover to make replacement.

Model with field diaphragm available.

A model with a built-in field diaphragm allows the use of Koehler
illumination. It features:
– A field lens unit with a field diaphragm

that has position-guide markings for
respective objectives.

– Easy and safe lamp replacement

procedure.

Improved

Wide variety of condensers

Abbe, Phase, and other Eclipse series
condensers, except the universal type, can be
used with the E200.

Phase contrast

Simple phase contrast observation at 10X,
20X and 40X is possible with a single
phase annulus slider.
The aperture diaphragm automatically
opens when the slider is inserted into the
condenser. Phase contrast 100X slider and
darkfield slider up to 40X
objectives are available as options.

Epi-fluorescence

The E200-dedicated epi-fluorescence attachment is available for users who want to begin
fluorescence observation. Although affordably priced, this attachment allows
comprehensive epi-fluorescence as well as UV excitation observations.

Simple polarizing

This method is ideal for observing amyloid and crystals.
To set up, install the polarizer over the field lens and the
analyzer.

Field diaphragm with position-guide
markings

E200-F

E2 Abbe condenser (left) and E2 phase
condenser

Simple polarizing set C

CFI Achromat DL objectives
for phase contrast

Filters are optional.

System Diagram

10 11

Accessories (cont’d.)

Ergonomic binocular tube

Drawing tube

Allows accurate
sketching of the
image being
observed.

Eye-level riser

Two risers can be inserted to
increase the eyepoint height—
25mm per riser for a total of
50mm.

Teaching heads

Face-to-face* and side-by-side teaching heads are available.

* Not recommended for use in conjunction with a photomicrographic system, because it makes the microscope top-heavy.

Object marker

Allows the point of interest within a specimen
to be marked with ink.

ND filter for objective lenses (ND3)

When this filter is used in combination with a 4x or 10x
objective lens, image brightness can be adjusted to nearly
equal to that of a 40x objective lens.

Digital Sight series digital
camera system

When the trinocular eyepiece tube is used, a
digital camera can be attached.* The DS-Fi1-L2,
a color camera head combined with a standalone
control unit, enables focusing and viewing of
images on the built-in 8.4-in. LCD monitor without

the need for a PC.
The optimum imaging conditions
that match the observation
technique in use can be
automatically set by clicking on

the appropriate “scene mode.”
Storing optimal images is easy.

*With a 4x objective lens, the image may show

uneven illumination depending on the camera used.

Plastic storage case

The rugged, lightweight storage case is
handy for transporting and storing the E200.

Both eyepiece length and tilt angle are adjustable.

* Not recommended for use in conjunction with

the Y-THF Teaching Unit, because it makes the
microscope top-heavy.

A

Y-IER Eye-level Riser

B

Intermediate Module

E2-FM Epi-Fl

C-SHG Mercury
Illuminator 50W

Intermediate Modules

Attachment

A

Drawing Tube

H-III

Photomicrographic System*

Projection Lens
PLI 2x, 2.5x, 4x, 5x

V-T Photo Adapter

Eyepiece TubesIntermediate Module Eyepieces

Digital Sight Series

Digital Camera

C-0.7x DXM

Relay Lens

Y-TV TV Tube

F

C-mount TV

Adapter A

C-mount

Adapter 0.55x

Y-TV 0.55x TV Tube

Digital Sight Series

F

Digital Camera

HH

A

D

E2-TB Binocular Tube E2-TF Trinocular Tube

A

A

Y-THS Teaching Unit B
(Side-by-Side)

C-TE Ergonomic Binocular Tube

(100/0, 50/50 or 100/0, 0/100)

Y-THP Pointer

Y-THPS
Teaching
Head
Support

H

C-TEP DSC
Port

Y-THF Teaching Unit
(Face-to-Face)*

CFI E10x

H

CFI E15x

A

*Not recommended for use in conjunction with a photomicrographic
system, because it makes the microscope top-heavy.

C

E2 Simple

ABCD

Analyzer

ND Filter for
Objective Lenses

CFI60 Objective Lens CFI60 Objective Lens

Dia. Polarizer

E

ABCD

E2 Simple

Analyzer

Dia. Polarizer

E

ND Filter for
Objective Lenses

Condensers

E2 Phase
Condenser

E

E2 Abbe
Condenser

Blue Filter

Phase Contrast
Slider 10x–40x

Phase Contrast
Slider 100x

Darkfield Slider

Diffuser Slider