Nikon C-HGF1, C-HGFIE User Guide

HG Precentered Fiber Illuminator

(manual)

(motorized)

HG Precentered Fiber Illuminator

Motorized model C-HGFIE available

The light intensity and shutter can be controlled from an
optional dedicated remote controller or a PC that incorporates
Nikon’s NIS-Elements imaging software. The light intensity and
shutter can be programmed for each application and controlled
in conjunction with the microscope and peripheral equipment.
This enables automatic control during excitation light
changeover and observation of multi-stained specimens.

Configured with ECLIPSE Ti-E

Remote controller

Light intensity control (manual)

Shutter (manual)

Difficult lamp alignment is a thing of the past

The Intensilight mercury-fiber illuminator employs a
precentered, long-life lamp that requires no centering
while allowing users to capture high-quality
fluorescence images with uniform brightness.

Precentered lamp requires no alignment

The use of a precentered lamp and dedicated optical fibers
eliminates the need for cumbersome centering and focusing
operations, even after the lamp is replaced. Uniform brightness is
always assured.

2000-hour long-life lamp

The lamp lasts an average of 2000 hours, or 10 times longer than
conventional mercury lamps. Therefore, replacement costs and
microscope downtime are greatly reduced.

Reduced heat and electrical noise

Dedicated optical fibers (1.5m, 3m) allow the light source to be
placed away from the microscope, reducing heat and electrical
noise on the microscope body. This is particularly suited to
long-time fluorescence observation (time-lapse observation) of
live cells.

Shutter and light intensity control

There are six levels of light intensity to choose from depending on
the specimen. The shutter allows light to the specimen to be
easily shut off without the power being turned off. Therefore,
photobleaching can be reduced to a minimum when observing
multi-stained specimens.

Greatly increased brightness for green
spectrum

At wavelengths of around 450nm to 500nm, brightness is much
higher than that of a conventional mercury lamp, making the
lamp ideally suited to observation of green fluorescence such as
FITC and GFP.

DC lighting for constant light intensity

As DC (direct current) lighting is less influenced by frequency than
AC (alternating current) lighting, DC provides constant, non­fluctuating light.

Safety measures

When the lamp replacement cover is open or the optical fibers
are not attached, the interlock automatically shuts the light off to
protect the user from possible light exposure. Furthermore, when
the lamp temperature sensor detects abnormally high
temperatures, power is cut to protect the lamp.

C-HGFI(manual)

C-HGFIE(motorized)

Spectrumcomparison

Relativeintensity

100.0

90.0

80.0

70.0

60.0

50.0

40.0

30.0

20.0

10.0

0.0

3

2

1

3

4

2

3

6

4

36

8

4

07

4

29

4

50

4

7

1

4

92

5

13

53

4

5

5

5

57

5

5

96

61

6

6

3

6

65

7

6

76

6

96

7

1

6

73

6

7

5

5

7

7

4

7

9

4

Wavelength(nm)

Intensilight
Mercurylamp

Time

0.00

80.00ms

Relativeintensity

DClighting

0.00 80.00ms

TypicalAClighting

Time

Relativeintensity

Off-center Precentered