Memmert INCO 246 User Manual

OPERATING MANUAL

CO2 incubators

INCO 108 INCO 108 med INCO 153 INCO 153 med INCO 246 INCO 246 med

Manufacturer and customer service

MEMMERT GmbH + Co. KG Postfach 17 20

D-91107 Schwabach

Äußere Rittersbacherstr. 38 D-91126 Schwabach Germany

Fon:	+49 (0)	09122	/ 925-0
Fax:	+49 (0)	09122	/ 14585
E-Mail: [e-mail]
Internet: www.memmert.com
Customer service:
Fon:	+49 (0)	09122/925-128
and:	+49 (0)	09122/925-126

E-Mail: [e-mail]

For service enquiries, please always specify the appliance number on the nameplate (see page 18).

© 2012 Memmert GmbH + Co. KG Edition 1/2012

Subject to modifications

About this manual

About this manual

Purpose and target group

This manual describes the setup, function, operation and maintenance of CO2 incubators of the types INCO and INCOmed with a chamber volume of 108, 153 and 246 litres. In this

manual, the term INCO is used for INCO as well as INCOmed for the purpose of simplification. Distinctions are explicitly pointed out.

This manual is intended for use by the trained staff of the operator in charge of operating and/or maintaining the incubator. If you are asked to work on the incubator, you should read this manual carefully before starting work on the unit. Familiarise yourself with the safety regulations. Only perform the work that is described in this manual. If there is something you don't understand, or certain information is missing, ask your superior or get in touch with the manufacturer. Do not do anything without authorisation.

Contents

The INCO incubator is available with different fittings: as a basic model, and with six different additional modules. The technical fittings and functional range of the basic model and the individual additional modules are described from page 11.

If specific equipment features or functions are available only with one of the additional modules, this is pointed out in the relevant sections in this manual.

Due to individual fittings, depictions in this manual may be different from the actual appearance.

Other documents that you must read:

► for service and repair work (see page 63) – a separate service manual

Storage and Forwarding

This instruction manual belongs with the incubator and should always be stored so that those who work on the incubator have access to it. It is the responsibility of the operator to ensure that persons who work on or who will work on the incubator are informed as to the whereabouts of this instruction manual. We recommend that it is always stored in a protected location close to the incubator. Make sure that the instruction manual is not damaged by heat or damp. If the incubator is sold on or transported and then set up again at a different location, this instruction manual must go with it.

3

Content

Content

1.	Safety regulations	6
1.1	Terms and icons used ..........................................................................................................	6
1.2	Product safety and dangers ................................................................................................	7
1.3	Requirements of the operating personnel..........................................................................	8
1.4	Responsibility of the owner.................................................................................................	8
1.5	Changes and conversions....................................................................................................	8
1.6	Behaviour in case of malfunctions and irregularities..........................................................	8
1.7	What to do in case of accidents..........................................................................................	9
1.8	Switching off incubator in an emergency...........................................................................	9
2.	Design and Function	10
2.1	Design................................................................................................................................	10
2.2	Function.............................................................................................................................	11
2.3	Optional extras ..................................................................................................................	11
2.4	Material..............................................................................................................................	13
2.5	Electrical equipment..........................................................................................................	13
2.6	Connections.......................................................................................................................	13
2.7	Intended use......................................................................................................................	14
2.8	EC Declaration of Conformity............................................................................................	16
2.9	Designation (nameplate)...................................................................................................	18
2.10	Technical data....................................................................................................................	18
2.11	Ambient conditions...........................................................................................................	20
2.12	Accessories included..........................................................................................................	20
3.	Delivery, Transport and Setting Up	21
3.1	Safety regulations..............................................................................................................	21
3.2	Transport............................................................................................................................	21
3.3	Delivery ..............................................................................................................................	21
3.4	Setup..................................................................................................................................	22
4.	Putting into Operation	24
4.1	Checks................................................................................................................................	24
4.2	Connecting ........................................................................................................................	24
4.3	Oxygen calibration.............................................................................................................	26
5.	Operation and control	27
5.1	Operating personnel..........................................................................................................	27
5.2	Opening the door ............................................................................................................	27
5.3	Loading the incubator.......................................................................................................	27
5.4	Inserting water tray(s) .......................................................................................................	28
5.5	Connect gas supply ...........................................................................................................	28
5.6	Switch on appliance ..........................................................................................................	28
5.7	Basic operation..................................................................................................................	29
5.8	Setting parameters............................................................................................................	29
5.9	Operating modes...............................................................................................................	30
5.10	Setting the operating mode..............................................................................................	30
5.11	During operation...............................................................................................................	36
5.12	Ending operation...............................................................................................................	39
6.	Warning messages and malfunctions	40
6.1	Warning messages ............................................................................................................	40
6.2	System/appliance errors ....................................................................................................	41
6.3	Power failure......................................................................................................................	44
4

		Content
7.	Advanced functions	45
7.1	Printer ...............................................................................................................................	45
7.2	Basic appliance settings (Setup)........................................................................................	45
7.3	Temperature monitoring ..................................................................................................	47
7.4	Sterilisation chipcard .........................................................................................................	51
7.5	User ID card (optionally available as an accessory)...........................................................	52
7.6	Calibration .........................................................................................................................	53
7.7	Communication interfaces ................................................................................................	58
7.8	Log memory ......................................................................................................................	62
8.	Maintenance and Servicing	63
8.1	Cleaning.............................................................................................................................	63
8.2	Regular maintenance.........................................................................................................	63
8.3	Adjusting door...................................................................................................................	64
8.4	Repairs and Service............................................................................................................	64
9.	Storage and Disposal	65
9.1	Storage ..............................................................................................................................	65
9.2	Disposal .............................................................................................................................	65
Index		66

5

Safety regulations

1.Safety regulations

1.1 Terms and icons used

In this manual, certain common terms and icons are used to warn you of dangers or to give you notes that are important in avoiding injury or damage. Observe and follow these notes and regulations to avoid accidents and damage. These terms and icons are explained below.

1.1.1 Terms used

"Warning" is always used whenever you or somebody else could be injured if you do not observe the accompanying safety regulation.

"Caution" is used for information that is important for avoiding damage.

1.1.2 Icons used

Prohibited sign (forbidding an action)

	Do not tilt	Do not lift appliance
	appliance
	Warning icons (warning of a danger)

	Danger of	Explosive	Danger of frost-
	electrical shock	atmosphere	bite/cold burns
	Warning of gas	heat/hot surfaces	Gas
	bottles
	Regulation signs (stipulate an action)
	Disconnect the	Wear gloves	Wear safety
	mains plug		shoes
	Observe	Two or more
	information in
		persons required
	separate manual
	Other icons
	Information on	First Aid:	Important or
			useful addition-
	first aid	Rinse eyes out
			al information

6

Safety regulations

1.2 Product safety and dangers

Incubators of the INCO type are technically well-developed, manufactured using high-quality materials and tested for many hours in the factory. They contain the latest technology and comply with recognised technical safety regulations. But there are still dangers involved, even when the appliance is used as intended. These dangers are described below.

Warning!

After removing covers, live parts may be exposed. You may receive an electric shock if you touch these parts. Disconnect the mains plug before removing any covers. Any work inside the unit may only be performed by qualified electricians.

Warning!

When loading the chamber with an unsuitable load, poisonous or

explosive vapours or gases may be produced. This could cause the chamber to explode, and people could be badly injured or poisoned.

The chamber may only be loaded with materials/test objects which

do not form any poisonous or explosive vapours when heated up

(see also Chapter 2.7 Intended use on page 14).

Warning!

Danger of suffocation. In high concentrations, CO2 and N2 can have

a suffocating effect. In normal operation, the incubator gives off small amounts of CO2 and – if equipped with the O2 module – N2 to its environment. You should therefore ensure that the room in

which it is installed is properly ventilated. If a gas bottle is not connected, or is empty, always close the stop valve or pressure reducer on the bottle.

Warning!

High concentrations of CO2 can cause cold burns or frostbite. Avoid contact with CO2 gas to the eyes and skin.

Warning!

CO2 gas bottles may burst or explode at high temperature. Do not use naked flames in the vicinity of the gas bottles. Store gas bottles at lower than 50 °C in a well-ventilated location. Prevent water from penetrating, as well as backflow into the gas bottles. It is essential that you read the safety notes and regulations of the gas suppliers.

CO2 and N2 are not dangerous substances in terms of the German Ordinance on Hazardous Substances (GefStoffV). You should nevertheless familiarise yourself with the applicable safety regulations prior to handling such gas bottles.

7

Safety regulations

1.3 Requirements of the operating personnel

The incubator may only be operated and maintained by persons who are of legal age, and who have received instructions for the incubator. Personnel who are to be trained, instructed or who are undergoing general training may only be active on the incubator under the continuous supervision of an experienced person.

The incubator may only be transported by persons (fork-lift truck, manual pallet jack), who are trained for this work and who know the corresponding safety regulations.

Repairs may only be performed by qualified electricians. In this case the regulations in the separate service manual must be observed.

1.4 Responsibility of the owner

The owner of the incubator

►is responsible for the flawless condition of the incubator and for the incubator being operated in accordance with its intended use (see page 14);

►is responsible for ensuring that persons who are to operate or service the incubator are qualified to do this, have received instructions about the incubator and are familiar with this operating manual;

►must know about the applicable regulations, requirements and work protection regulations, and train staff acordingly;

►is responsible for ensuring that unauthorised persons have no access to the incubator;

►is responsible for ensuring that the maintenance plan is adhered to and that maintenance and repair work is properly carried out (see page 63);

►ensures, for example through corresponding instructions and inspections, that the incubator and its surroundings are kept clean and tidy;

►is responsible for ensuring that personal protective clothing is worn by operating personnel, e.g. work clothes, safety shoes, protective gloves.

1.5 Changes and conversions

No independent conversions or alterations may be made to the incubator. No parts may be added or inserted which have not been approved by the manufacturer.

Independent conversions or alterations result in the EC declaration of conformity (see page 16) losing its validity, and the incubator may no longer be operated.

The manufaturer is not liable for any damage, danger or injuries that result from independent conversions or alterations, or from non-observation of the regulations in this manual.

1.6 Behaviour in case of malfunctions and irregularities

The incubator may only be used when in a flawless condition. If you as the operator notice irregularities, malfunctions or damage, immediately put the incubator out of service (see Chapter 1.8) and inform your superiors.

You can find information on eliminating malfunctions from page 40.

8

Safety regulations

1.7What to do in case of accidents

1.Keep calm. Act resolutely and with consideration. Pay attention to your own safety.

2.Switch off the incubator and close the valves on the gas bottle.

3.Call a doctor.

4.Initiate first aid measures. If available: Call a trained first aid helper.

In case of contact with CO2 to the eyes and skin:

Rinse eyes out with water for at least 15 minutes. With cold burns, rinse with water for at least 15 minutes. Cover over in a sterile manner. Call a doctor.

Inhaling CO2 or N2:

High concentrations can cause suffocation. Symptoms may include a loss of mobility and consciousness. The victim is not aware of suffocating.

Low concentrations of CO2 can cause accelerated breathing and headaches.

Anyone affected should breathe fresh air, using a breathing device independent of recirculating air. Keep the person warm and calm. Call a doctor. In case of respiratory arrest, use artificial respiration.

If gas is escaping:

Leave the room immediately, warn others and ventilate the room. If you re-enter the room, use an autonomous breathing device (independent of ambient air) if it has not been established that the atmosphere is harmless.

1.8 Switching off incubator in an emergency

► Push main switch on front side of appliance

(Fig. 1). ► Close the valves on the gas bottle.

Fig. 1 Switch off incubator by pressing the main switch

9

Design and Function

2.Design and Function

2.1 Design

1
2							18
3
4

5

6

7

17

8	16

9

10

11

12

13				15

14

Fig. 2		Design of INCO incubators
1	Controller/control panel (see page 29)		10	Sliding shelf
2	Set key		11	Door seal
3	Push/turn control		12	Ventilator/inlet opening
4	Connections on the rear of the oven		13	Heating ribs
	(see page 13)		14	Adjustable feet
5	Heating ribs (see page 11)		15	Nameplate (beneath door, see page 18)
6	Pt100 temperature sensors		16	Door knob (see page 27)
7	Humidity sensor		17	Door
8	Glass door		18	Chip card reader
9	Chamber seal
10

Design and Function

2.2 Function

The air in the incubator is heated up by an all-round heater with a large surface (Fig. 3, No. 1). The CO2 and/or N2 gas is introduced into the working chamber via a sterile filter. Because CO2 has a much higher specific weight than air, the gas is let into the working chamber above the interior fan (2). The turbulence-free interior ventilation (3) ensures a uniform distribution of the gases, creating a homogenous atmosphere.

In the basic version, humidification takes place by means of water trays (4). To avoid uncontrolled condensation, dosed fresh air is piped into the interior. If the appliance is equipped with a humidity module, humidification takes place via a hot-air generator, which allows water to evaporate at a set rate. The sterile hot air is let into the interior above the fan and is mixed with the air current (5).

1			1
	5
1	1	1	1
3	2
4	4		1

Fig. 3 How INCO incubators work

2.3 Optional extras

2.3.1 Basic equipment

►Electronic fuzzy-supported PID process controller with pulse width modulation and permanent performance adjustments and time-saving self-diagnosis system to quickly locate errors (see page 41)

►All-round heater with larger surface area, with additional thermal conduction layer (see Fig. 3)

►Capacitive humidity sensor

►Humidity control (fresh air via sterile filter) ensures that setpoint humidity is quickly reached and guarantees short recovery times, while avoiding condensation formation

►Homogenous atmosphere and temperature distribution through encapsulated, turbu- lence-free ventilation system

►STERICard for fully automatic sterilisation process control for hot air sterilisation of appliance, including sensors and fan rotor (see page 51)

11

Design and Function

►Digitalised electronic CO2 control with automatic zero position, NDIR measuring system with self-diagnosis system and acoustic error display, air pressure compensation

►Language settings (see page 46)

►Alphanumeric text display

►Integrated week time switch with grup function (e.g. each working day) (see page 33)

►Retracting push-turn control for simple operation of appliance (see page 28)

►Two separate Pt100 temperature sensors DIN Class A in a 4-wire circuit for control and monitoring

►Digital monitoring control for overtemperature, undertemperature and automatic setpoint following (ASF) (see page 50)

►Mechanical temperature limiter (TB protection class 1, see page 47)

►Monitoring relay to switch off heater in case of error

►Optical alarm display

►Acoustic signal messages if temperature or CO2 limits are crossed, when door is open and if gas bottle is empty (see page 40)

►Calibration of temperature, humidity, CO2 and O2 possible on the device without a separate PC (see from page 53)

2.3.2 Optionally available additional modules

Comfort module:

►Two gas connections with quick release connectors (see page 13)

►Automatic switch-over of gas bottles

Hygiene module

► Electro-polished, seamless laser-welded chamber

Communication module

►Logging option of temperature, CO2 and relative humidity via computer/laptop

►Internal log memory with 1024 kB as ring memory for all setpoint and actual values, errors and settings in real time and with date, logging approx. 3 months at 1 minute storage interval (see page 62)

►Parallel printer port (PCL3-compatible) for printing out log data (see page 13 and page 45)

►optionally USB, Ethernet, RS-232 or RS-485 interface (see from page 58)

CO2 module

►Extended adjustment range from 0 to 20 %

►3-point calibration (5 %, 10 %, 15 % CO2)

O2 module

►Control of oxygen concentration through the introduction of nitrogen (N2); adjustment range 1 % to 20 % O2 (not in combination with Comfort or Premium module)

Premium module

► Includes comfort, hygiene, communication and CO2 modules

Humidity module

► Active microprocessor humidification and dehumidification control (40-97 % rh)

12

Design and Function

2.4 Material

For the outer housing, MEMMERT uses stainless steel (W.St.No. 1.4016), and for the interior, stainless steel (W.St.No. 1.4301), which stands out through its high stability, optimal hygienic properties and corrosion-resistance towards many (but not all!) chemical compounds (caution for example with chlorine compounds). The chamber load for the appliance must be carefully checked with respect to chemical compatibility with the materials mentioned.

A material resistance table can be requested from the manufacturer.

2.5 Electrical equipment

►Operating voltage: See nameplate (page 18), 50/60 Hz

►Current consumption: See nameplate (page 18)

►Protection class 1, i.e. operating insulation with safety earth terminal in accordance with EN 61010

►Protection type IP 20 acc. to EN 60 529

►Interference-suppressed acc. to EN 55011 class B

►Appliance fuse: Fusible link 250 V/15 A quick-blow

►The temperature controller is protected with a miniature fuse 100 mA (200 mA at 115 V)

►For models with a humidity model the CO2 controller is protected with a 6.3 A miniature fuse

2.6 Connections

1

H2O

7	In
	N2

2

Fig. 4 Connections on rear of appliance

3

4

5

6

1Water connection (only for model with humidity module)

2Mains lead

3USB connection (only for models with communication or premium modules; alternatively Ethernet, RS-232 or RS-485 connection) (details from page 58)

4Printer connection (only for models with communication or premium modules) (see page 45)

5CO2 connection to main gas bottle

6CO2 connection to reserve gas bottle (only for models with CO2 or premium modules)

7N2 connection (quick-release connector, only if equipped with O2 module)

13

Design and Function

2.6.1 Electrical connection

Observe the country-specific regulations when making connections (e. g. in Germany DIN VDE 0100 with residual current device).

This appliance is intended for operation on an electrical power system with a system impedance Zmax at the point of transfer (service line) of a maximum of 0.292 ohm. The operator must ensure that the incubator is operated only on an electrical power system that meets these requirements. If necessary, you can ask your local energy supply company what the system impedance is.

2.6.2 Connection of external appliances

Only appliances may be connected externally (depending on the model, USB, RS 232, RS 485, Ethernet, printer) whose interfaces comply with the requirements for safety extra-low voltage (e.g. PC).

2.6.3 Gas connection

The oven can be connected with the supplied compressed air hose via a pressure regulator with gas bottle monitor (DIN 8546) to a CO2 compressed gas bottle or directly to a central CO2 gas supply.

For models with the CO2 or premium modules, two gas bottles with quick release connectors can be connected. If equipped with the O2 module, an N2 gas bottle can be connected instead of a second CO2 gas bottle (Fig. 4).

The pre-pressure must not exceed 1.2 bar. A value between 0.8 and 1 bar is considered to be an ideal value.

2.6.4 Water connection

For models with a humidity module, the incubator can be connected with the supplied hose to the also supplied water supply tank.

2.7 Intended use

INCO incubators may only be used for incubating cell cultures or similar. Any other use is improper, and may result in hazards and damage.

The incubators are not explosion-proof (they do not comply with workplace health & safety regulation VBG 24). The chambers may only be loaded with materials and substances which cannot produce any toxic or explosive vapours at temperature ranges up to 50 ºC, and which themselves cannot explode, burst or ignite.

The incubators may not be used for drying, vaporising and branding paints or similar materials, the solvents of which could form an explosive mixture when combined with air. If there is any doubt as to the composition of materials, they must not be loaded into the incubator. Potentially explosive gas-air mixtures must not be produced, either in the interior of the chamber or in the direct vicinity of the appliance.

The incubator may not be used for sterilisation purposes. It is not a steriliser with respect to the Law on Medical Devices.

Only gas bottles with a pressure regulator may be connected to the gas connections of the oven. Introducing other gases or materials than CO2 or N2 is not permitted.

14

Design and Function

2.7.1 Intended use of the INCOmed

For INCOmed incubators, which are subject to the 93/42/EEC guideline (Council Directive on the approximation of the laws of the Member States relating to medical devices), the intended use is defined as follows:

The CO2 incubator INCO med is intended for the creation and maintenance of constant environmental conditions for application in the field of in vitro fertilisation (IVF), especially for the incubation of oocytes, spermatozoa and zygotes in special culture dishes for IVF application as well as for gene expression and the biosynthesis of RNA and proteins.

15

Design and Function

2.8 EC Declaration of Conformity

EC Declaration of Conformity

Manufacturer´s name and address:	MEMMERT GmbH + Co. KG
	Äußere Rittersbacher Straße 38
	D-91126 Schwabach
Product:	CO2 Incubator
Type:	INCO 2
Sizes:	108 l / 153 l / 246 l
Nominal voltage:	AC 230 V 50/60 Hz

The designated product is in conformity with the European EMC-Directive

2004/108/EEC

including amendments

Council Directive of 03 May 1989 on the approximation of the laws of the Member States relating to electromagnetic compatibility.

Full compliance with the standards listed below proves the conformity of the designated product with the essential protection requirements of the above-mentioned EC Directive:

DIN EN 61326-1:2006-10	EN 61326-1:2006
DIN EN 61000-3-11:2001-04	EN 61000-3-11 :2000

The designated product is in conformity with the European Low Voltage Directive

2006/95/EEC

including amendments

Council Directive on the approximation of the laws of the Member States relating to Electrical equipment for use within certain voltage limits.

Full compliance with the standards listed below proves the conformity of the designated product with the essential protection requirements of the above-mentioned EC Directive:

DIN EN 61 010-1 (VDE 0411 part 1):2002-08	EN 61 010-1:2001
DIN EN 61 010-2-010 (VDE 0411 part 2-010):2004-06	EN 61 010-2-010:2003

Schwabach, 22.06.10

______________________________

(Legally binding signature of the issuer)

This declaration certifies compliance with the above mentioned directives but does not include a property assurance. The safety note given in the product documentation which are part of the supply, must be observed.

Modelljahr 2006 D10318 / 22.06.10

16

Design and Function

EC Declaration of Conformity

Manufacturer's name and address:	MEMMERT GmbH + Co. KG
	Äußere Rittersbacher Straße 38
	91126 Schwabach, Germany
Product:	Incubators
Type:	INCO med
Sizes:	108 l / 153 l / 246 l
Rated voltage:	AC 230 V 50/60 Hz

This product complies with the provisions of the directive:

93/42/EEC

including annex and revisions

Council Directive on the approximation of the laws of the Member States relating to medical devices of 14 June 1993 (Official Journal of the EC No. L 169, page 1 of 12 July 1993)

Schwabach, Germany, 01/11/2012

(legally binding signature of the manufacturer)

This declaration states the compliance with the above Directives, however, does not provide any warranted properties. The safety instructions in the supplied documents have to be observed.

D24002

17

Design and Function

2.9 Designation (nameplate)

The nameplate (Fig. 5) provides information about the appliance model, manufacturer and technical data. It is attached to the front of the appliance, on the right beneath the door (see page 10).

1

2

3

4

5

Typ: INC 108	F.-Nr.: 0109.0088	10
230 V~	4.4 A 50/60 Hz 1000 W	9
DIN12880-Kl.3.1	Nenntemp.: 50 °C	8
		7

	6
Fig. 5		Nameplate
1	Type designation		6	Address of manufacturer
2	Operating voltage		7	Disposal note
3	Applied standard		8	Temperature range
4	Protection type		9	Connection / performance values
5	CE conformity		10	Factory number

2.10 Technical data

Model	108	153	246
* See Fig. 6 on page 20.
Chamber width A* [mm]	560	480	640
Chamber height B* [mm]	480	640	640
Chamber depth C* [mm]	400	500	600
Appliance width D* [mm]	710	630	790
Appliance height E* (varies	778	920	938
due to adjustable feet) [mm]
Appliance depth F* (includ-	590	690	790
ing door handle) [mm]
Chamber volume [litres]	108	153	246
Weight [kg]	70	90	110
Performance [W]	1000	1500	2000
Max. number of sliding	-/4	-/6	2 x 6/6
shelves half size / full size

18

Design and Function

Model

108

153

246

* See Fig. 6 on page 20.

Max. load per sliding shelf	15	15	15
[kg]
Max. load per appliance [kg]	40	40	60
Temperature	Temperature recording by means of Pt100 in a 4-wire circuit
	Adjustment range: Normal mode: 20 °C to 50 °C
	Sterilisation mode: 160 °C (4 hours) via STERICard
	Adjustment precision: 0.1 °C
	Control range: from 8 °C above room temperature to 50 °C
	Variation (time): max. ±0,1 °C at 37 °C
	Variation (spatial): max. ±0.3 °C at 37 °C
Humidity	The relative humidity in the chamber is measured by a capac-
	itive humidity sensor and displayed digitally in percent.
	The measurement precision of the humidity sensor is 1 % rh
	► Adjustment range: 88 to 97 % rh (for models with
	humidity module 40 to 97 % rh)
	► Adjustment precision: 1 % rh
	► Display range: 10 to 98 % rh
	► Variation (time): max. ±1 % rh
CO2	The CO2 content is determined through an NDIR measur-
	ing system, controlled constantly by a microprocessor and
	displayed digitally in percent
	► Adjustment range: 0 to 10 % (for models with CO2 or
	premium modules 0 to 20 %)
	► Adjustment precision: 0.1 %
	► Variation (time): max. ±0.1 % rh
	► Variation (spatial): max. ±0.3 %
O2 (only if equipped with O2	The O2 content is determined with a long-lasting, mainte-
module)	nance-free zirconium dioxide sensor, constantly controlled by
	a microprocessor and displayed digitally in percent
	► Adjustment range: 1 to 20 %
	► Adjustment precision: 0.1 %
	► Variation (time): max. ±0.1 %
	► Variation (spatial): max. ±0.3 %

19

Design and Function

75	D	75	F	38
	A		C
			38	202
		E		B
		8		76
		12

Fig. 6 Dimensions of INCO incubators

2.11 Ambient conditions

►The incubator may only be used in enclosed rooms and under the following environmental conditions:

Ambient temperature: 5 ºC to 35 ºC

Humidity:	max. 80 % not condensing
Degree of pollution:	2
Altitude of installation	max. 3,000 m above sea level

►The incubator may not be used in areas where there is a risk of explosions. The ambient air must not contain any explosive dusts, gases, vapours or gas-air mixtures. The incubator is not explosion-proof.

►Heavy dust production or aggressive vapours in the vicinity of the appliance could lead to sedimentation in the chamber interior and as a consequence, could result in short circuits or damage to electrical parts. For this reason, sufficient measures should be taken to prevent large clouds of dust or aggressive vapours from developing.

2.12 Accessories included

For incubators with basic fittings:

►Two (INCO 108) or three (INCO 153 and 246) sliding shelves

►Gas pressure hose

►Water tray (one for INCO 108 and 153 incubators, two for INCO 246 incubators)

►Sterilisation chipcard

Additionally, for models with humidity module:

► Water supply tank and connection hose

Additionally, for models with CO2 or premium module:

► Second gas pressure hose with quick-release connector

Additionally, for models with O2 module:

► Second gas pressure hose with quick-release connector

20

Delivery, Transport and Setting Up

3.Delivery, Transport and Setting Up

3.1 Safety regulations

Warning!

You may injure your hands or feet when transporting and installing the incubator. You should wear protective gloves and work shoes.

Warning!

Because of the weight of the incubator, you could injure yourself if

you try to lift it on your own.

If possible, only transport the incubator with a fork-lift truck or manual pallet jack. The incubator may only be moved using a means of transport by persons who have the required qualification for this (e.g. fork-lift truck licence). This incubator may not be transported with a crane.

If the incubator has to be carried, at least two people are required for models 108 and 153, and at least four people for model 246.

Warning!

The incubator could fall over and seriously injure you. Never tilt the

incubator and transport it only in an upright position.

3.2 Transport

The incubator can be transported in three ways:

►with a fork-lift truck; move the forks of the truck entirely under the incubator

►on the manual pallet jack.

►by carrying; to do this, at least two people are required for models 108 and 153, and four people for model 246. Read the weight information detailed on page 18.

3.3 Delivery

The incubator is delivered in cardboard packaging on a cardboard pallet.

3.3.1 Unpacking

1.Remove cardboard packaging or cut open carefully along an edge.

2.Lift up incubator from pallet and put down on the appliance feet.

3.3.2 Checking for completeness and transport damage

►Check the delivery note to ensure that the delivery is complete.

►Check the inside and outside of the incubator for damage.

If you notice deviations from the delivery note, damage or irregularities, do not put the incubator into operation, but inform the haulage company and the manufacturer.

3.3.3 Disposing of packaging material

Dispose of the packaging material (cardboard) in accordance with the effective legal disposal regulations for cardboard packaging in your country.

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