Leica TCS STED User Manual

Leica TCS STED

The Fast Track to Superresolution

Technical Documentation

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1 Inverted research microscope Leica DMI6000 CS

2 Scan head

3 Laser and power supply

4 Computer table
5 Air damped optical table

6 Control panel

7 Supply control
8 STED module

9 Housing for 592 nm STED depletion laser

10 APD detection unit (optional, recommended)
11 Microscope control

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STED Module

Mechanics ultra stable and compact device, fi rmly fi xed to scanner
STED Laser: excitation: internal Argon gas lasers (continuous wave), variable excitation wavelength

depletion: 592 nm visible fi ber laser (continuous wave)

Optomechanics used imaging port UV-port (no UV available)

modulation of depletion PSF, automated beam adjustment for perfect alignment
of excitation and depletion laser, average duration: < 1 min., alignment inside the
scanhead, no illumination by lasers of the sample during alignment

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