Leica TCS SPE User Manual
Spectacular Imaging!
Leica TCS SPE: High Resolution Spectral Confocal
Affordable Excellence for Your Daily Research
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Spectacular Imaging
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Easy to Achieve
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A Reliable System
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Affordable Excellence
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Confocal microscopy applications have in-
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creased greatly in the last decade, and high
quality fluorescent images have been an important key to new discoveries. In addition, clinical,
pharmaceutical and biotechnological research
shows a growing demand for high-resolution 3D
images. Confocal systems offer the advantage
of the best image quality, but most instruments
currently offered still require intensive training
before they can be usefully operated. They also
need specific room conditions not found in
every facility.
Spectacular
Imaging
Affordable Excellence
for your Daily Research
To make confocal technology accessible to a
wide range of users in their daily research, we
have developed the Leica TCS SPE, a high resolution spectral confocal, easy to use, extremely
compact and robust – yet still affordable.
Without compromising our innovative concepts
and high quality standards, the Leica TCS SPE
offers outstanding spectral detection technology developed and patented by Leica Microsystems. New optical concepts for true colocalization plus a new Leica software platform
common to our complete range of confocal
and widefield products, simplify operation and
reduce training time. You’ll find it all in the
Leica TCS SPE.
The highly integrated Leica TCS SPE has all
you need for your daily research. It is optimized
for applications such as live cell imaging and
morphological studies in small research groups
and multi user environments.
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The advantages of true confocal imaging
Spectacular Imaging
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3D confocal imaging
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Variable motorized pinhole to
match objectives
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488, 532 and 635 nm excitation
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405 nm excitation for
nuclear stainings
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Low-noise solid state lasers
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Highly efficient prism for
emission spectrometer
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High dynamic photomultiplier
with photon booster technology
“Brilliant images, great
technology and excellent
cost performance ratio –
Leica’s TCS SPE
is the ideal tool for our
laboratory.”
Jean-Luc Vonesch
Head of Imaging Center at the IGBMC (INSERM,
CNRS, ULP), Strasbourg-Illkirch, France
The Confocal Principle
Detector
Confocal pinhole
Laser
Beam
Splitter
Objective
Focal plane
out
in
out
Scanning the specimen in thin optical layers and detecting the
fluorescence signal point by point results in images free from
the stray light of adjacent elements. The result – brilliant images
at very high resolution. The information from each signal in
each optical section is reconstructed by intelligent software
into excellent 3D images, resolving the smallest detail of the
specimen’s structure.
Crystal clear 3D confocal images are our standard
Our confocal systems are famous for crystal clear high-resolution images with minimum noise, due to high performance
components unrivalled in the confocal field. We have integrated
this highly innovative and unsurpassed technology into the new
Leica TCS SPE. To ensure high quality imaging, optimal excitation
is provided by up to four low noise solid-state lasers with 488,
532 and 635 nm excitation lines for common dyes. The broad
range of applications is extended by the 405 nm option for
nuclear staining.
Ultra-high sensitivity with maximum spectral efficiency is ensured
by our spectrophotometer based on a prism, which spreads the
light into its spectrum. Maximum signal strength and optimal
resolution is provided by a special high dynamics photomultiplier with photon booster technology, usually found in systems
of a higher price range. The continuously adjustable pinhole
diameter automatically adapts to all objectives. Channel multiplexing by sequential scanning prevents any cross-talk of dyes
and results in excellent dye separation. Optimally integrated with
our high performance research microscopes and optics, we
fully harness our own technology to deliver the best images.
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