Leica TCS SP5 X User Manual

Leica TCS SP5 X
X-citing Life – The Only Confocal That Tunes Into Any Excitation
Ready for any dye the future brings
The only system that fully adapts to the sample
Full freedom and flexibility in emission AND excitation
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Biological and medical research fluorescence imaging has evolved from studying one or two features in a specimen to examining even more features and their dynamic interactions in the cellular environment. This requires a variety of fluorescent dyes to study four, five, or more pro­teins simultaneously. As new dyes are devel­oped and used, the ability to optimally excite and detect these fluorophores is necessary in both living and fixed specimen.
Until now, research in multi-user facilities or institutes has been limited by the type or num­ber of dyes that could be excited. Conventional laser scanning imaging systems are equipped with a set of gas or solid state lasers that emit fixed laser lines for use. Using this equipment the researcher has to choose the dyes and labels for detection according to the imaging system. The experiment has to be compromized to the available instrumentation.
Leica TCS SP5
X-citing Life!
The Leica TCS SP5 X supercontinuum confocal unites the broadband capabilities of the Leica TCS SP5 AOBS®and the freedom and flexibility to select any excitation line within the continu­ous range of 470 to 670 nm.
With our patented spectral detection technolo­gy you have complete freedom to choose the detection area in up to five confocal PMT chan­nels. The acousto-optical beam splitter AOBS is the only option for a supercontinuum laser, and allows the selection of up to eight simulta­neous lines from anywhere in the white light spectrum.
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