Leica TCS SP5 II User Manual

Page 1
System Overview TCS SP5 II
Leica TCS SP5 II, TCS SP5 MP, TCS SP5 X, FLIM, SMD
Page 2
22
0005MD/0006MD acieL
Stands
Leica DMI600 0
Microscope table
passive upright
156504701
Microscope table
active upright
156504702
DM6000 CS 5 pos fluo
155931620
DM6 000 CS 8 pos fluo
155931625
DM6 000 CS 5 pos fluo
155931624
DM500 0 CS 5 pos fluo
155931521
IR Optical table
DM6000 CFS
156502563
Objective Revolver, Nosepiece H, 6 pos.
15501249
Optical outfit DM6000CFS, incl. cond.
156500612
BF Detector Dodt
contrast
156504203
Data Aquisition Unit
156504762
Trigger Unit
156504760
20x Objective Slider
15555089
HCX APO 20x/1.0
Objective
15507701
Microscope table
active inverted
156504712
Manual Dodt
Gradient System
156504204
DM6000CFS w. Objective Revolver and MechStage w/o Rotary
155931623
DM6000CFS w. 20x
Objective and
MechStage w/o Rotary
155931622
Motorized Dodt
Gradient System
156504205
System Overview TCS SP5 II
EL60 00 Optical Out fit DM6 000
156500613
EL60 00 Optical Out fit DM6 000 w. DIC
156500614
EL60 00 Optical Out fit DM5 000
156500603
EL60 00 Optical Out fit DM5 000 w. DIC
156500604
EL60 00 Optical Out fit DMI6000
156500620
EL60 00 Optical Out fit DMI6000
w. DIC
156500621
DMI6 000 CS Bino
155933633
DMI6 000 CS Trino
155933621
DMI6 000 CS BPTrino
155933634
Microscope table
passive inverted
156 504711
Microscope table
passive inverted
156 504711
Leica DM6000 CFS
Page 3
3
Scanhead Options
Imaging Laser AOBS
R 156502100 RB 156502105 RGB 156502110 RYB 156502115 RYBV 156502116 ROB 156502120 ROGB 156502123 ROYB 156502125 ROYBV 156502126
SP5 X Basic module
156 501102
Imaging WLL w.
Ar ion L aser
156502148
Basic module
AOBS
156 501100
Tandem scanning
system SP 5 II
156 50112 2
Conventional
scan-
ning system SP5 II
156501120
Resonant scanning
system SP 5 II
156 501121
MFP and IR Lasers
Confocal detector
FLIM
156501391
Confocal detector
imaging
156501390
Exhaust w. silencer
Ar Laser
156502210
X1 Port Options
Control Panel w.
programmable LCD
156504750
Trigger unit
156504760
NDD Detector
options
UV and U V/4 05
options
Imaging WLL w.
B Diode (48 8 nm)*
156502147
Imaging WLL
156502149
Imaging Laser Dichroic
R 156502150 G 156502151 RB 156502155 RGB 156502160 RYB 156502165 RYBV 156502166 ROB 156502170 ROGB 156502173 ROYB 156502175 ROYBV 156502176
Basic module
dichroic
156501101
SP Channel 01 156501310 SP Channel 02 156501320 SP Channel 03 156501330 SP Channel 04 156501340 SP Channel 05 156501350
*available Jan. 2 010
Switchable
Beam expander
156502220
Notch filter vis .
156504506
Page 4
44
A
Leica DM6000/DM5000Leica DMI600 0
Stages and Inserts
Upright Stands
Scanning stage
upright
156504150
Motor stage
upright*
156504155
T
156 50112 2
Inser t fix
Type S
156504103
Inser t universal
Type S
156504105
Inser t rotatable
Type S
156504104
Super Z Galvo stage
Type H
156 504116
T
156 50112 2
Manual stage*
15522019
Inver ted Stands
Motor stage
inverted
156504145
T
156 50112 2
Inser t fix
Type H
156 504113
156 501121
Inser t universal
Type H
156504115
156 50112 2
Plate inser t
Super Z Microtiter
156504420
Inser t rotatable
Type H
156 504114
T
156 50112 2
Petri dish
inser t 36 mm
156504136
156 501121
Inser t Super Z
Willco dish 3 5 mm
156604421
Petri dish
inser t 39 mm
156504139
T
156 50112 2
Inser t Super Z
Willco dish 5 0 mm
156604422
T
156 50112 2
T
156 50112 2
Heating mount
frame AK 160 x 116
156506040
T
SysteP5
156 50112 2
Super Z Galvo Stage
Type H
156 504116
T
156 50112 2
Inser t rotatable
Type H
156 504114
T
156 50112 2
Inser t universal
Type H
156504115
T
156 50112 2
Inser t fix
Type H
156 504113
T
156 50112 2
Universal Spec.
holder uprigh t
15650 4171
T
156 50112 2
Inser t fix
Type S
156504103
156 501121
Inser t universal
Type S
156504105
Inser t rotatable
Type S
156504104
Super Z Galvo stage
Type S
156504102
Stage B mot. 72 38
Step
15501250
Luigs & Neumann
Stage in vitro
156504160
B
DM6000 CFS
including
Mech. stage w/o
rotar y
DM6000 CFS
Scanning stage
156504161
Scanning stage
universal
156504141
Manual stage
15501234
Super Z Galvo stage
Type S
156504102
Inser t Super Z
Mat tek dish 35 mm
156604423
System Overview TCS SP5 II
Luigs & Neumann
Stage in vivo
156504162
* Defau lt
Leica DM6000 CFS
Page 5
5
B
A
11600183
Glass plate 160 x 116 x 3 mm
11600185
Holder for slide frames 70 x 70 mm
15600234
Universal holding frame
15532363
Inser t for Petri dish 36 mm , mod.
15532364
Inser t for Petri dish 54 mm, mod.
15532096
Scan Insert for 35 mm W illco dish
15532095
Scan Insert for 50 mm W illco dish
15532338
Universal holder for Microtiter plate
UV and Laser Options
UV/4 05 Port
156501201
Pulsed 40 5 Laser
156502305
Tandem Scanning
156501122
UV Port
156 501211
UV Laser AOTF incl.
156502325
11600184
Holder for glass slides 3” x 1”
156504171
Universal Specim en holder
Attenuation F W405
156502351
AOT F 405 Internal
156502352
40 5 Laser
156502300
UV Laser and 4 05
LaserAOT F incl.
156502326
156506040
Heatable mounting frame AK 160 x 16
Page 6
66
With Super ZWithout Super Z
POC mini Cell
cultivation system
15521739
POC-R Cell
cultivat ion system
156506020
Perfusion inser t for POC
mini (open w. cover)
15521740
Perfusion inser t for
POC-R (open w. cover)
15521752
POC mini Cell
cultivation system
15521739
POC-R Cell
cultivat ion system
156506020
Perfusion inser t open w. cover for POC mini
15521740
Perfusion inser t open
w. cover for POC -R
15521752
Super Z Galvo stage
Type H
156 504116
System Overview TCS SP5 II
Super Z Incubation system ex t. (22 0 V)
156506062
Super Z Incubation
system ex t. (110 V)
156506061
Petri dish attach-
ment 3 5 mm Super Z
156506063
Petri dish attach-
ment 5 0 mm Super Z
156506064
Attachment for
chamber slide Super Z
156506065
Super Z Incubation
system basic
156506060
Super Z Galvo stage
Type H
156 504116
Attachment for cover
glass chamber Super Z
156506066
Attachment for slide
glas Super Z
156506067
Ludin chamber set
(18 mm) for DMI6 000
156506070
Ludin chamber set
(12 mm) for DMI6 00 0
156506071
Holder for Ludin chambers
for Super Z and Insert rot.
156506073
Holder for Ludin chambers
Adapter for Super Z
156507074
Holder for Ludin chambers
w/o Super Z
156507072
On-s tage incubation
system complete
156506010
Heating inser t P
156506014
Cool/ Heat
inser t P
156506015
On-s tage incubator
(Incubator S)
156506013
Temperature con trol
for objective heater
156506030
Objective heater
d = 29 mm
156506031
Objective heater
d = 30.5 mm
156506032
Page 7
7
Climate chambers for DM6000 CFS
Laser safety box L&N DM6 000 CFS,
black
156506009
Laser safety box manual
& scanning stage DM6 000 CFS, black
156506056
Climate chambers for DMI6 000
Ludin Cube 2 & Box DMI6 000,
bright
156506007
Passive gas regulation LUDIN cube
& box without LUDIN incubator kit
156506051
Active CO2 regulation LUDIN cube
& box without LUDIN incubator kit
156506050
O2 controller 2– 60 %
for LUDIN active
156506004
O2 controller 0.5 –25 %
for LUDIN active
156506006
Auxiliar y out let
LUDIN active regulation
156506005
Incubator kit LUDIN
for use w ithout Super Z
156506053
Incubator kit LUDIN
for use w ith Super Z
156506052
Environmental control
Software Licences
Live Data Mode156503201 3D Visualisation Colocalisation
156503203
Deconvolution
156503204
Microlab
156503205
Offline package
156503206
Electrophysiology
156503250
156503209
PC and monitors
Workstation 2 x LCD156503121: Workstation Plus 2x LCD156503122: Workstation 3 0“ monitor156503123: Workstation Plus 30“ monitor156503124:
Scanhead
AOBS
Scanhead
dichroic
Trigger unit
156504760
X1 Port
emission access
156504500
X1 Port for
ROI
156504533
Spectrometer
156505500
ROI Spectrometer
Page 8
88
IR Black optical table DMI600 0
90 0 x 1500
156502560
MP Picosecond: Lasers and beam routing
IR Black optical table DM 60 00
90 0 x 120 0
156502561
IR Black optical table*
150 0 x 24 00
156502562
High power lasers
Tandem Scanning
System SP5
156501122
IR Laser MaiTai HP
156502503
IR Laser Chameleon
Ultra II
156502508
Tandem Scanning
System SP5
156501122
IR Laser Chameleon
Ultra
156502506
IR Imaging por t.
mult ifunct. upright HX
156501213
IR Imaging por t.
mult ifunct. inver ted HX
156501203
IR Imaging por t.
mult ifunct. CFS HX
156501214
IR Beam routing
EOM & at t. DM6 000
156502527
IR Beam routing
EOM & at t. DMI60 00
156502522
IR Beam r outing
EOM & att.DM6000 CFS
156502524
IR Tables
sresal rewop woL
IR Laser MaiTai
Wideband
156502501
IR Laser MaiTai
156502500
IR Laser MaiTai
Broadband
156502502
IR Black optical table DM 60 00 CFS
and ST ED 12 00 x 1500*
156502563
IR Beam routing
EOM & at t. DM6 000
156502527
IR Beam routing
att. DM 6000
156502528
IR Beam routing
att. DM 6000
156502523
IR Beam routing
EOM & at t. DMI60 00
156502522
System Overview TCS SP5 II
IR Imaging por t.
multifunct. upright
156501212
IR Imaging por t.
multifunct. inverted
156501202
Resonant Scanning
System SP5
156501121
IR Laser Chameleon
Ultra I
156502507
IR Imaging por t.
mult ifunct. CFS HX
156501214
IR Beam routing
EOM & at t. DM6 000 CFS
156502524
* actively damped
Page 9
9
IR Tables
IR Black optical table DM60 00
90 0 x 1200
156502561
IR Black optical table DMI600 0
90 0 x 1500
156502560
IR Black optical table for double
MP system 150 0 x 2400*
156502562
IR Black optical table DM60 00 CFS
and STED 120 0 x 1500
156502563
MP: Leica femtosecond lasers and beam routing
High power lasers
IR Laser MaiTai HP
156502513
Tandem Scanning
System SP5
156501122
IR Laser Chameleon
Ultra
156502516
Optical adapter
MaiTai HP Leica
156502565
Optical adapter
Chameleon Leica
156502566
Optical adapter MaiTai
HP other supplier
156502568
High power laser
purchased direc tly
from laser supplier
by customer
Optical adapter Cha-
meleon other supplier
156502569
IR Imaging por t multi-
funct. inverted HX
156501203
IR Imaging por t multi-
funct. upright H X
156501213
IR Imagi ng port mul ti-
func t. CFS H X
156501214
IR Beam routing
EOM & at t. DMI60 00
156502522
IR Beam routing
EOM & at t. DM6 000
156502527
IR Beam r outing
EOM & att.DM6000 CFS
156502524
Resonant Scanning
System SP5
156501121
IR Laser Chameleon
Ultra I
156502517
IR Laser Chameleon
Ultra II
156502518
Low power lasers
Tandem Scanning
System SP5
156501122
IR Laser MaiTai
broadband
156502512
Optical adapter
MaiTai Leica
156502567
Optical adapter MaiTai
other supplier
156502571
Low power laser
purchased directly
from laser supplier
by customer
IR Imaging por t multi-
funct. CF S HX
156501214
IR Imaging por t multi-
funct. upright
156501212
IR Imagi ng port mul ti-
funct. inverted
156501202
IR Beam routing
EOM & at t. DM6 000
156502527
IR Beam routing
att. DM 6000
156502528
IR Beam routing
att. DMI600 0
156502523
IR Beam routing
EOM & at t. DMI60 00
156502522
IR Beam r outing
EOM & att.DM6000 CFS
156502524
Resonant Scanning
System SP5
156501121
IR Laser MaiTai
156502514
IR Laser MaiTai
wideband
156502519
* actively damped
Page 10
1010
4 channel NDD Box
w
ith liquid light guide
156504270
NDD detection unit
156504253
Tandem Scanning
System SP5
156501122
Empty filter cube
156504240
Resonant Scanning
System SP5
156501121
Dapi/ TRITC
filter cube
156504242
Dapi/FITC filter cube
156504241
Tandem Scanning
System SP5
156501122
Detection unit is needed if more than one detector (NDD or T L-BF) is used.
Resonant Scanning
System SP5
156501121
FITC/TXR filter cube
156504244
CFP/YFP
filter cube
156504243
DMI6000 TLD
1 channel
156504221
DM6 000 CFS TL D
1
channel incl.
mirror housing
156504227
DM6 000 CFS RL D
1 channel
156504236
DM6000 RLD
2 channels
156504234
DMI6000 TLD
2 channels
156504223
DM6 000 CFS TL D 2
channels incl. dichroic
156504226
DM6 000 CFS TL D 2 ch.
incl. mirror housing
156504225
DMI6000 RLD
2 channels
156504233
DM6 000 CFS RL D
2 channels
156504235
Filter cubes for 2 NDDs
Tandem Scanning
System SP5
156501122
Empty filter cube
156504247
Resonant Scanning
System SP5
156501121
SHG440/CFP/YFP/
DsRed filter cube
156504246
Dapi/FITC/ TXR
Alexa633 filter cube
156504245
Filter cubes for 4 NDDs
DM6 000 CFS Opt ical and
mechanical adaption TLD 4 channels
156504278
DM6 000 Optical and mechanical
adaption RL D 4 channels
156504276
DMI6 000 Optical and mechanical
adaption RL D 4 channels
156504274
DM6 000 CFS Opt ical and mechanical
adaption RL D 4 channels
156504279
DMI6 000 Optical and mechanical
adaption TLD 4 channels
156504275
MP: NDDs
System Overview TCS SP5 II
DM6000 CFS TLD
1 channel
156504228
DMI6000 RLD
1 channel
156504231
DM6000 RLD
1 channel
156504232
DM6000 TLD
1 channel
156504222
FITC/T RITC filter cube is always included wi th 2 NDDs
Page 11
11
TCS SMD FCS (PQ)
SMD detection package
FCS (high time resolut ion,
2 MPD SPA D)
156505200
SMD detection package
FCS (high sensitivi ty,
2 PE AP Ds)
156505201
Second license for SP T-1 (FCS)
156505280
Scanning stage
incl. Super Z
Super Z
Galvo s tage
X1 Port
Emission access
156504500
Conventional
scanning system
156501120
Tan dem
scanning system
156 50112 2
Basic module
AOBS + Imaging
laser AOBS
SP Channel 01 – 05
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505252
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
Obj HCX PL APO
63 x/1.2 0 W CORR
CS, 0.14 –0.18
15506279
Page 12
1212
SMD MF P port for MP
156505270 156505271 156505272 156505273
for INV. for INV. HX for UPR. for UPR. HX
TCS SMD FLIM: Internal SP FL IM (PQ)
SMD detection package
(spect ral FLIM
2 SP FL IM PMT)
156505205
SMD detection package
(spect ral FLIM
1 SP FL IM PMT)
156505204
Second license
for SP T-1+2
(FC S + FLIM)
156505282
IR Beam
routing
IR
Laser
IR Black
optical table
Basic module
dichroic + Imaging
laser dichroic
Basic module
AOBS + Imaging
laser AOBS
Scanning stage
incl. Super Z
Super Z
Galvo s tage
Trigger unit
156504760
UV/4 05 Port
156501201
Correction lenses
optional
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw)
156505221
SMD Excitation
package (405 + 470
+ 64 0, all pulsed)
156505223
SMD E xcitation pack.
(405, pulsed+ cw; 470 + 6 40, pulsed)
156505224
SMD Excitation
package
(470 + 64 0, pulsed)
156505222
SMD Excitation
package
(MP)
156505225
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw))
156505221
Polarization
filter wheel
for 405
156505261
Polarization
filter wheel
for 405 + 470 + 640
156505260
System Overview TCS SP5 II
SP Channel 01 – 05
Correction lenses
UV/4 05 Port
156501201
No
Yes
MP?
Please order exactly one SMD detection package! An SMD excitation package is not necessary for FCS. Resonant scanning system 156501121 cannot be used for FCS.
Page 13
13
SMD MF P port for MP
156505270 156505271 156505272 156505273
for INV. for INV. HX for UPR. for UPR. HX
TCS SMD FLIM: External F LIM (PQ)
SMD detection
package
external FLIM
(basic, 1 P MT)
156505202
SMD detection
package
external FLIM
(high time
resolution,
2 MPD SPA D)
156505203
Second license
for SP T-2
(FLIM)
156505281
IR Beam
routing
IR
Laser
IR Black
optical table
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505252
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
SMD Filter cube
156505240 156505253 156505254 156505255 156505256
Empty UV DAPI FITC TRITC
Scanning stage
incl. Super Z
Super Z
Galvo s tage
X1 Port
Emission access
156504500
Trigger unit
156504760
UV/4 05 Port
156501201
Correction lenses
optional
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw)
156505221
SMD Excitation
package (405 + 470
+ 64 0, all pulsed)
156505223
SMD E xcitation pack.
(405, pulsed+ cw; 470 + 6 40, pulsed)
156505224
SMD Excitation
package
(470 + 64 0, pulsed)
156505222
SMD Excitation
package
(MP)
156505225
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw))
156505221
No
Yes
Correction lenses
UV/4 05 Port
156501201
Basic module
dichroic + Imaging
laser dichroic
Basic module
AOBS + Imaging
laser AOBS
SP Channel 01 – 05
MP?
Page 14
1414
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505252
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
SMD MF P port for MP
156505270 156505271 156505272 156505273
for INV. for INV. HX for UPR. for UPR. HX
TCS SMD FLCS: FCS + External F LIM + Internal SP FLIM (PQ)
SMD detection package external FLIM + FC S (high
time resolution,
2 MPD SPA D)
+ spect ral FLIM
(2 SP FLIM PMT)
156505207
Second license
for SP T-1+2
(FC S + FLIM)
156505282
IR Beam
routing
IR
Laser
IR Black
optical table
Basic module
AOBS + Imaging
laser AOBS
Scanning stage
incl. Super Z
Super Z
Galvo s tage
Trigger unit
156504760
Conventional
scanning system
156501120
Tan dem
scanning system
156 50112 2
X1 Port
Emission access
156504500
Trigger unit
156504760
UV/4 05 Port
156501201
Correction lenses
optional
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw)
156505221
SMD Excitation
package (405 + 470
+ 64 0, all pulsed)
156505223
SMD E xcitation pack.
(405, pulsed+ cw; 470 + 6 40, pulsed)
156505224
SMD Excitation
package
(470 + 64 0, pulsed)
156505222
SMD Excitation
package
(MP)
156505225
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw))
156505221
Polarization
filter wheel
for 405
156505261
Polarization
filter wheel
for 405 + 470 + 640
156505260
Obj. HCX PL APO
63 x/1.2 0 W CORR
CS, 0.14 –0.18
15506279
System Overview TCS SP5 II
SP Channel 01 – 05
Correction lenses
UV/4 05 Port
156501201
No
Yes
MP?
Please order exactly one SMD detection and one SMD excitation package! Only exception: Package 156 505220 or 156505221 can be combined wi th 15 65 05225. Resonant scanning system 156501121 cannot be used for FLCS.
Page 15
15
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505252
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
SMD MF P port for MP
156505270 156505271 156505272 156505273
for INV. for INV. HX for UPR. for UPR. HX
TCS SMD FLCS: FCS + External F LIM (PQ)
SMD detection
package external
FLIM + FC S (high
time resolution,
2 MPD SPA D)
156505206
Second license
for SP T-1+2
(FC S + FLIM)
156505282
IR Beam
routing
IR
Laser
IR Black
optical table
Basic module
AOBS + Imaging
laser AOBS
Scanning stage
incl. Super Z
Super Z
Galvo s tage
Trigger unit
156504760
Conventional
scanning system
156501120
Tan dem
scanning system
156 50112 2
X1 Port
Emission access
156504500
Trigger unit
156504760
UV/4 05 Port
156501201
Correction lenses
optional
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw)
156505221
SMD Excitation
package (405 + 470
+ 64 0, all pulsed)
156505223
SMD E xcitation pack.
(405, pulsed+ cw;
470 + 6 40, pulsed)
156505224
SMD Excitation
package
(470 + 64 0, pulsed)
156505222
SMD Excitation
package
(MP)
156505225
SMD Excitation
package
(405, pulsed)
156505220
SMD Excitation
package
(405, pulsed + cw))
156505221
Obj. HCX PL APO
63 x/1.2 0 W CORR
CS, 0.14 –0.18
15506279
SP Channel 01 – 05
Correction lenses
UV/4 05 Port
156501201
No
Yes
MP?
Please order exactly one SMD detection and one SMD excitation package! Only exception: Package 156 505220 or 156505221 can be combined wi th 15 65 05225. Resonant scanning system 156501121 cannot be used for FLCS.
Page 16
1616
FLIM (B & H)
Resonant Scanning
System SP5
156 501121
Confocal detector
Imaging
156501390
Basic module
dichroic + Imaging
laser dichroic
X1 Port
Emission access
156504500
X1 Por t adaption multipurpose for FCS/AP D imaging
and FL IM
156504531
X1 Por t adaption
for FLIM
156504532
SP Channel 01 – 05
andem Scanning
System SP5
IR Imaging port
Multifunc.
System SP5
156 50112 2
UV/4 05 Port
156501201
andem Scanning
System SP5
IR Beam routing
Multifunc.
Pulsed laser 4 05
156502305
SP FL IM Adaptor k it
156505351
Confocal detector
FLIM
156501391
Basic module
AOBS + Imaging
laser AOBS
Trigger unit
156504760
System Overview TCS SP5 II
Maximum of 2 internal FL IM detectors and 1 external FLIM detector
Internal FLIM
detection
External FLIM
detection
Exci tation MP
Exci tation 4 05
FLIM and FCS
FLIM only
APD imaging
Basic module
dichroic + Imaging
laser dichroic
X1 Port
Emission access
156504500
X1 Por t adaption
for APD imaging
and FC S
156504530
SP Channel 01 – 05
Basic module
AOBS + Imaging
laser AOBS
Page 17
17
Coupling FLIM MP
156505350
APD Detection unit
156505130
B&H routing is needed if more than one FLIM detector is used.
System SP5
156 501122
IR Laser
System SP5
156 501122
IR Black
optical table
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505252
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
FLIM Filter cube M
156504580 156504581 156504582 156504583 156504584
MP FL IM m D FLIM m DAPI m FITC m TRI TC m
B&H SPC 8 30 incl.
PC and software
156505301
B&H Routing for
4 channel detection
156505340
B&H FLIM detector
PM C-10 0
156505330
APD
imaging
FLIM
FLIM
APD Detection unit
156505130
SMD Filter cube
156505240 156505241 156505242 156505243 156505244 156505245 156505246 156505247 156505248 156505249 156505250 156505251 156505552
Empty Alexa 40 5/cy2 DAPI/At to 647 CFP/YFP CFP/RFP Cy2/Cy5 GFP narrow/RFP GFP wide/ RFP FITC/TRITC GFP/HcRed Alexa 48 8/Alexa 6 33 Neutral splitting Polarization splitting
FLIM Filter cube M
156504560 156504561 156504562 156504563 156504564
MP FL IM D FLIM DAPI FITC TRITC
Page 18
1818
Leica TCS SP5 II
Leica TCS SP5 X
Page 19
19
Leica DM6000 CFS
Leica TCS SPS SMD
Page 20
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