Leica TCS SP5 AOBS User Manual

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AOBS

Leica TCS SP5: The Broadband Confocal

High Sensitivity for High-Quality Results

Thousands of applications

The applications of fluorescence microscopy have enormously in­creased during the last five decades. The growing variety of different
fluorochromes featuring different excitation and emission properties
caused an increasing demand of new fluorescence filters and dich­roics. The benefits of the numerous stains call for successful missions,
but the numberless filters are rather inconvenient for researchers, too.
Here, a broadband confocal microscope – covering all requirements
by a single device – means a true relief.

Light – a precious matter

Sensitivity tips the scales

Efficiency is the key to success. Not only in science and economy –
where it turns to profitability, but as well in research and routine labora­tories, where productivity has always been a strong requirement.
Efficiency strongly depends on the suitability and the capabilities of the
instrumentation. What does this mean in terms of confocal fluorescence
microscopy? Here, first and foremost, efficiency is determined by the
sensitivity of the detection device. Of course, speed, flexibility and the
ergonomics are important parameters, though but the principle purpose
of the device is to guide the maximum number of photons, emitted by the
fluorochromes in the sample, safely to the detector.

Drosophila melanogaster larvae (eye-disc)
Green: RNA binding protein (nuclei) Alexa 488
Red: Axons, Cy 3
Blue: Axon endings of MJ94-positive neurons, Cy 5
Courtesy of Dr. Christoph Melcher, Research Center Karlsruhe,
Institute for Toxikology and Genetics, Eggenstein-Leopoldshafen, Germany