Leica TCS SMD User Manual

Leica TCS SMD Series

Single Molecule Detection Platform

Technical Documentation

General Specifications of SMD Prepared Leica TCS SP5

Microscopes

Microscope anti-vibration table

Z-drive

Continuous wave lasers

Upright

Inverted

Specifi cation For imaging

Vibration insulation Passive

SuperZ galvanometer stage 1500 m travel range/3 nm stepsize

Motorfocus (stand)

Laser type For imaging

VIS

UV Diode, 50 mW: 405 nm

Laser type For imaging

IR

Leica DM6000 CS
Leica DM6000 CFS
Leica DMI6000 CS

Leica DMI6000 CS bottom port

Travel range depending on mechanics
of microscope/15 nm step size

Diode, 40 mW
Ar, 65 mW: 458, 476, 488, 496, 514 nm
HeNe, 1 mW: 543 nm
HeNe, 2 mW: 594 nm
HeNe, 10 mW: 633 nm
DPSS, 20 mW: 561 nm

iSa 1.2 ps 1 W 690...1040 nm

T
(various ranges)

: 442 nm

Pulsed lasers

Excitation modulation

VIS

UV –

Modulation type For imaging

AOTF VIS Up to 8 channels
AOTF UV
EOM IR Yes
Pulsed laser driver Optional

Up to 3 channels

–

–

2

Additional SMD Specific Specifications

TCS SMD FCS TCS SMD FLIM TCS SMD FLCS

Yes Yes Yes
Yes Yes Yes
Yes, recommended Yes Yes, recommended

Yes Yes Yes

For FCS For FLIM For FCS and FLIM

Passive Passive Passive

1500 m travel range/3 nm stepsize
Travel range depending on mechanics

of microscope/15 nm step size

For FCS No cw laser excitation for FLIM For FCS

Diode, 40 mW: 442 nm – Diode, 40 mW: 442 nm
Ar

, 65 mW: 458, 476, 488, 496, 514 nm – Ar, 65 mW: 458, 476, 488, 496, 514 nm
HeNe, 1 mW: 543 nm – HeNe, 1 mW: 543 nm
HeNe, 2 mW: 594 nm – HeNe, 2 mW: 594 nm
HeNe, 10 mW: 633 nm – HeNe, 10 mW: 633 nm
DPSS, 20 mW: 561 nm – DPSS, 20 mW: 561 nm

–––

Pulsed excitation for FCS not required For FLIM For FLIM and FLCS

–

–

–

–

1500 m travel range/3 nm stepsize 1500 m travel range/3 nm stepsize
Travel range depending on mechanics

of microscope/15 nm step size

iSa 1.2 ps 1 W 680 ... 1040 nm

T
(various ranges)

Diode, up to 3 mW average @ 40 MHz,
470 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps
(depending on selected power level)

Diode, up to 4.5 mW average @ 40 MHz,
640 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps
(depending on selected power level)

Diode, up to 3 mW average @ 40 MHz,
405 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps
(depending on selected power level)

Travel range depending on mechanics
of microscope/15 nm step size

TiSa 1.2ps 1 W 680 ... 1040 nm
(various ranges)

Diode, up to 3 mW average @ 40 MHz,
470 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps
(depending on selected power level)

Diode, up to 4.5 mW average @ 40 MHz,
640 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps
(depending on selected power level)

Diode, up to 3 mW average @ 40 MHz,
405 nm (software controlled selection of pulse
frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps
(depending on selected power level)

For FCS For FLIM For FCS and FLIM

Up to 8 channels – For FCS: 8 channels

–––
–
– For VIS and UV FLIM: up to 3 channels For VIS and UV FLIM: up to 3 channels

Optional for MP FLIM Optional for MP FLIM

3

General Specifications of SMD Prepared Leica TCS SP5

Features For imaging

Number of laser ports Up to 3 (UV - VIS - IR)

Optics

Scanner

Number of lasers

Excitation – emission splitting

Detection range 400...800 nm

UV and IR imaging Sequential or simultaneous

Field upgradable To IR: yes

UV correction

Pinhole Alignment stable single pinhole

Pinhole diameter control

Scanner design For imaging

Scanning concept Optically correct scanning at low inertia

Switch conventional – resonant scanner

Conventional scanner For imaging (PMT and APD)

Maximal line frequency 2800 Hz
Minimal line frequency
Scan speed granulation 1400
Maximal frame rate 512 x 512 5 Hz
Maximal frame rate 512 x 16 50 Hz
Beam park Yes
Maximal frame resolution 8192 x 8192 pixel
Scan zoom 1.0 ... 64 x
Panning Yes
Field rotation 200° optical
Field diameter 22 mm

Up to 8

Acousto Optical Beam Splitter (AOBS
or dichroic beam splitters

Individual precise correction optics
(up to 6 positions)

Motorized by software,
automatic mode available

Conventional and resonant scanner
in one system (optional)

1 Hz

®

)

4

Additional SMD Specific Specifications

TCS SMD FCS TCS SMD FLIM TCS SMD FLCS

For FCS For FLIM For FCS and FLIM

1 (VIS) 2 (UV & IR, or UV & VIS)

Up to 8

AOBS For pulsed lasers: dichroic mirrors

Depending on fi lter cube used

––

To FCS: yes To FLIM: yes –

UV not required for FCS Required for UV FLIM To FLCS: yes

Alignment stable single pinhole Alignment stable single pinhole Required for UV FLIM
Motorized by software,

automatic mode available

For FCS For FLIM For FCS and FLIM

Beam park Optically correct scanning at low inertia

Conventional scanner required

Up to 3

Internal SP FLIM: 400 … 800 nm
External FLIM: Depending on fi lter cube used

Motorized by software,
automatic mode available

Conventional and resonant scanner in one
system (optional)

For FCS: 1 (VIS)
For FLIM: 2 (UV & IR, or UV & VIS)

For FCS: up 8,
For FLIM: up to 3

For FCS: AOBS,
For pulsed lasers: dichroic mirrors

Depending on fi lter cube used

Internal SP FLIM: 400 … 800 nm
External FLIM, FCS, and FLCS: Depending on
fi lter cube used

Alignment stable single pinhole

FCS: Beam park
FLIM: optically correct scanning at low inertia

Conventional scanner required, resonant
scanner optional

No scanning during FCS measurement For FLIM data acquisition For FLIM data acquisition

– 1400 Hz 1400 Hz
–
– 1400 1400
– 5 Hz 5 Hz
– 50 Hz 50 Hz
– Yes Yes
– 512 x 512 pixel 512 x 512 pixel
– 1.0 ... 64 x 1.0 ... 64 x
–no no
– 200° optical 200° optical
– 22 mm 22 mm

1 Hz 1 Hz

5