Leica TCS SMD User Manual

Leica TCS SMD Series

Single Molecule Detection Platform

Technical Documentation

General Specifications of SMD Prepared Leica TCS SP5

Leica DM6000 CS

Upright

Leica DM6000 CFS

	Microscopes
		Inverted	Leica DMI6000 CS
			Leica DMI6000 CS bottom port
	Microscope anti-vibration table	Specification	For imaging
		Vibration insulation	Passive
		SuperZ galvanometer stage	1500 μm travel range/3 nm stepsize
	Z-drive
		Motorfocus (stand)	Travel range depending on mechanics
			of microscope/15 nm step size
		Laser type	For imaging
		VIS	Diode, 40 mW: 442 nm
			Ar, 65 mW: 458, 476, 488, 496, 514 nm
	Continuous wave lasers		HeNe, 1 mW: 543 nm
			HeNe, 2 mW: 594 nm
			HeNe, 10 mW: 633 nm
			DPSS, 20 mW: 561 nm
		UV	Diode, 50 mW: 405 nm
		Laser type	For imaging
		IR	TiSa 1.2 ps 1 W 690...1040 nm
			(various ranges)
			–
	Pulsed lasers	VIS
			–

	UV	–
	Modulation type	For imaging
	AOTF VIS	Up to 8 channels
Excitation modulation
	AOTF UV	Up to 3 channels
	EOM IR	Yes
	Pulsed laser driver	Optional

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Additional SMD Specific Specifications

	TCS SMD FCS	TCS SMD FLIM	TCS SMD FLCS
	Yes	Yes	Yes
	Yes	Yes	Yes
	Yes, recommended	Yes	Yes, recommended
	Yes	Yes	Yes
	For FCS	For FLIM	For FCS and FLIM
	Passive	Passive	Passive
	1500 μm travel range/3 nm stepsize	1500 μm travel range/3 nm stepsize	1500 μm travel range/3 nm stepsize
	Travel range depending on mechanics	Travel range depending on mechanics	Travel range depending on mechanics
	of microscope/15 nm step size	of microscope/15 nm step size	of microscope/15 nm step size
	For FCS	No cw laser excitation for FLIM	For FCS
	Diode, 40 mW: 442 nm	–	Diode, 40 mW: 442 nm
	Ar, 65 mW: 458, 476, 488, 496, 514 nm	–	Ar, 65 mW: 458, 476, 488, 496, 514 nm
	HeNe, 1 mW: 543 nm	–	HeNe, 1 mW: 543 nm
	HeNe, 2 mW: 594 nm	–	HeNe, 2 mW: 594 nm
	HeNe, 10 mW: 633 nm	–	HeNe, 10 mW: 633 nm
	DPSS, 20 mW: 561 nm	–	DPSS, 20 mW: 561 nm
	–	–	–
	Pulsed excitation for FCS not required	For FLIM	For FLIM and FLCS
	–	TiSa 1.2 ps 1 W 680 ... 1040 nm	TiSa 1.2ps 1 W 680 ... 1040 nm
		(various ranges)	(various ranges)
		Diode, up to 3 mW average @ 40 MHz,	Diode, up to 3 mW average @ 40 MHz,
	–	470 nm (software controlled selection of pulse	470 nm (software controlled selection of pulse
		frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps	frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps
		(depending on selected power level)	(depending on selected power level)
		Diode, up to 4.5 mW average @ 40 MHz,	Diode, up to 4.5 mW average @ 40 MHz,
	–	640 nm (software controlled selection of pulse	640 nm (software controlled selection of pulse
		frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps	frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps
		(depending on selected power level)	(depending on selected power level)
		Diode, up to 3 mW average @ 40 MHz,	Diode, up to 3 mW average @ 40 MHz,
	–	405 nm (software controlled selection of pulse	405 nm (software controlled selection of pulse
		frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps	frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps
		(depending on selected power level)	(depending on selected power level)
	For FCS	For FLIM	For FCS and FLIM
	Up to 8 channels	–	For FCS: 8 channels
	–	–	–
	–	Optional for MP FLIM	Optional for MP FLIM
	–	For VIS and UV FLIM: up to 3 channels	For VIS and UV FLIM: up to 3 channels

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General Specifications of SMD Prepared Leica TCS SP5

		Features	For imaging
		Number of laser ports	Up to 3 (UV - VIS - IR)
		Number of lasers	Up to 8
		Excitation – emission splitting	Acousto Optical Beam Splitter (AOBS®)
			or dichroic beam splitters
		Detection range	400...800 nm
	Optics
		UV and IR imaging	Sequential or simultaneous
		Field upgradable	To IR: yes
		UV correction	Individual precise correction optics
			(up to 6 positions)
		Pinhole	Alignment stable single pinhole
		Pinhole diameter control	Motorized by software,
			automatic mode available
		Scanner design	For imaging
		Scanning concept	Optically correct scanning at low inertia
		Switch conventional – resonant scanner	Conventional and resonant scanner
			in one system (optional)
		Conventional scanner	For imaging (PMT and APD)
		Maximal line frequency	2800 Hz
		Minimal line frequency	1 Hz
	Scanner
		Scan speed granulation	1400
		Maximal frame rate 512 x 512	5 Hz
		Maximal frame rate 512 x 16	50 Hz
		Beam park	Yes
		Maximal frame resolution	8192 x 8192 pixel
		Scan zoom	1.0 ... 64 x
		Panning	Yes
		Field rotation	200° optical
		Field diameter	22 mm

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Additional SMD Specific Specifications

	TCS SMD FCS	TCS SMD FLIM	TCS SMD FLCS
	For FCS	For FLIM	For FCS and FLIM
	1 (VIS)	2 (UV & IR, or UV & VIS)	For FCS: 1 (VIS)
			For FLIM: 2 (UV & IR, or UV & VIS)
	Up to 8	Up to 3	For FCS: up 8,
			For FLIM: up to 3
	AOBS	For pulsed lasers: dichroic mirrors	For FCS: AOBS,
			For pulsed lasers: dichroic mirrors
	Depending on filter cube used	Internal SP FLIM: 400 … 800 nm	Depending on filter cube used
		External FLIM: Depending on filter cube used
			Internal SP FLIM: 400 … 800 nm
	–	–	External FLIM, FCS, and FLCS: Depending on
			filter cube used
	To FCS: yes	To FLIM: yes	–
	UV not required for FCS	Required for UV FLIM	To FLCS: yes
	Alignment stable single pinhole	Alignment stable single pinhole	Required for UV FLIM
	Motorized by software,	Motorized by software,	Alignment stable single pinhole
	automatic mode available	automatic mode available
	For FCS	For FLIM	For FCS and FLIM
	Beam park	Optically correct scanning at low inertia	FCS: Beam park
			FLIM: optically correct scanning at low inertia
	Conventional scanner required	Conventional and resonant scanner in one	Conventional scanner required, resonant
		system (optional)	scanner optional
	No scanning during FCS measurement	For FLIM data acquisition	For FLIM data acquisition
	–	1400 Hz	1400 Hz
	–	1 Hz	1 Hz
	–	1400	1400
	–	5 Hz	5 Hz
	–	50 Hz	50 Hz
	–	Yes	Yes
	–	512 x 512 pixel	512 x 512 pixel
	–	1.0 ... 64 x	1.0 ... 64 x
	–	no	no
	–	200° optical	200° optical
	–	22 mm	22 mm

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