Leica TCS SMD Series
Single Molecule Detection Platform
Technical Documentation
General Specifications of SMD Prepared Leica TCS SP5
Leica DM6000 CS
Upright
Leica DM6000 CFS
Microscopes |
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Inverted |
Leica DMI6000 CS |
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Leica DMI6000 CS bottom port |
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Microscope anti-vibration table |
Specification |
For imaging |
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Vibration insulation |
Passive |
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SuperZ galvanometer stage |
1500 μm travel range/3 nm stepsize |
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Z-drive |
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Motorfocus (stand) |
Travel range depending on mechanics |
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of microscope/15 nm step size |
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Laser type |
For imaging |
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VIS |
Diode, 40 mW: 442 nm |
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Ar, 65 mW: 458, 476, 488, 496, 514 nm |
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Continuous wave lasers |
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HeNe, 1 mW: 543 nm |
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HeNe, 2 mW: 594 nm |
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HeNe, 10 mW: 633 nm |
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DPSS, 20 mW: 561 nm |
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UV |
Diode, 50 mW: 405 nm |
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Laser type |
For imaging |
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IR |
TiSa 1.2 ps 1 W 690...1040 nm |
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(various ranges) |
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– |
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Pulsed lasers |
VIS |
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UV |
– |
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Modulation type |
For imaging |
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AOTF VIS |
Up to 8 channels |
Excitation modulation |
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AOTF UV |
Up to 3 channels |
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EOM IR |
Yes |
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Pulsed laser driver |
Optional |
2
Additional SMD Specific Specifications
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TCS SMD FCS |
TCS SMD FLIM |
TCS SMD FLCS |
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Yes |
Yes |
Yes |
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Yes |
Yes |
Yes |
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Yes, recommended |
Yes |
Yes, recommended |
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Yes |
Yes |
Yes |
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For FCS |
For FLIM |
For FCS and FLIM |
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Passive |
Passive |
Passive |
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1500 μm travel range/3 nm stepsize |
1500 μm travel range/3 nm stepsize |
1500 μm travel range/3 nm stepsize |
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Travel range depending on mechanics |
Travel range depending on mechanics |
Travel range depending on mechanics |
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of microscope/15 nm step size |
of microscope/15 nm step size |
of microscope/15 nm step size |
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For FCS |
No cw laser excitation for FLIM |
For FCS |
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Diode, 40 mW: 442 nm |
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Diode, 40 mW: 442 nm |
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Ar, 65 mW: 458, 476, 488, 496, 514 nm |
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Ar, 65 mW: 458, 476, 488, 496, 514 nm |
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HeNe, 1 mW: 543 nm |
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HeNe, 1 mW: 543 nm |
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HeNe, 2 mW: 594 nm |
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HeNe, 2 mW: 594 nm |
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HeNe, 10 mW: 633 nm |
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HeNe, 10 mW: 633 nm |
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DPSS, 20 mW: 561 nm |
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DPSS, 20 mW: 561 nm |
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– |
– |
– |
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Pulsed excitation for FCS not required |
For FLIM |
For FLIM and FLCS |
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TiSa 1.2 ps 1 W 680 ... 1040 nm |
TiSa 1.2ps 1 W 680 ... 1040 nm |
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(various ranges) |
(various ranges) |
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Diode, up to 3 mW average @ 40 MHz, |
Diode, up to 3 mW average @ 40 MHz, |
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470 nm (software controlled selection of pulse |
470 nm (software controlled selection of pulse |
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frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps |
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 500 ps |
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(depending on selected power level) |
(depending on selected power level) |
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Diode, up to 4.5 mW average @ 40 MHz, |
Diode, up to 4.5 mW average @ 40 MHz, |
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640 nm (software controlled selection of pulse |
640 nm (software controlled selection of pulse |
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frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps |
frequency: 5, 10, 20, 40 MHz), < 90 ps ... < 400 ps |
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(depending on selected power level) |
(depending on selected power level) |
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Diode, up to 3 mW average @ 40 MHz, |
Diode, up to 3 mW average @ 40 MHz, |
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405 nm (software controlled selection of pulse |
405 nm (software controlled selection of pulse |
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frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps |
frequency: 5, 10, 20, 40 MHz), < 70 ps ... < 300 ps |
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(depending on selected power level) |
(depending on selected power level) |
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For FCS |
For FLIM |
For FCS and FLIM |
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Up to 8 channels |
– |
For FCS: 8 channels |
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– |
– |
– |
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Optional for MP FLIM |
Optional for MP FLIM |
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For VIS and UV FLIM: up to 3 channels |
For VIS and UV FLIM: up to 3 channels |
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3
General Specifications of SMD Prepared Leica TCS SP5
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Features |
For imaging |
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Number of laser ports |
Up to 3 (UV - VIS - IR) |
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Number of lasers |
Up to 8 |
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Excitation – emission splitting |
Acousto Optical Beam Splitter (AOBS®) |
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or dichroic beam splitters |
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Detection range |
400...800 nm |
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Optics |
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UV and IR imaging |
Sequential or simultaneous |
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Field upgradable |
To IR: yes |
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UV correction |
Individual precise correction optics |
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(up to 6 positions) |
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Pinhole |
Alignment stable single pinhole |
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Pinhole diameter control |
Motorized by software, |
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automatic mode available |
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Scanner design |
For imaging |
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Scanning concept |
Optically correct scanning at low inertia |
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Switch conventional – resonant scanner |
Conventional and resonant scanner |
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in one system (optional) |
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Conventional scanner |
For imaging (PMT and APD) |
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Maximal line frequency |
2800 Hz |
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Minimal line frequency |
1 Hz |
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Scanner |
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Scan speed granulation |
1400 |
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Maximal frame rate 512 x 512 |
5 Hz |
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Maximal frame rate 512 x 16 |
50 Hz |
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Beam park |
Yes |
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Maximal frame resolution |
8192 x 8192 pixel |
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Scan zoom |
1.0 ... 64 x |
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Panning |
Yes |
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Field rotation |
200° optical |
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Field diameter |
22 mm |
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4
Additional SMD Specific Specifications
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TCS SMD FCS |
TCS SMD FLIM |
TCS SMD FLCS |
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For FCS |
For FLIM |
For FCS and FLIM |
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1 (VIS) |
2 (UV & IR, or UV & VIS) |
For FCS: 1 (VIS) |
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For FLIM: 2 (UV & IR, or UV & VIS) |
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Up to 8 |
Up to 3 |
For FCS: up 8, |
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For FLIM: up to 3 |
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AOBS |
For pulsed lasers: dichroic mirrors |
For FCS: AOBS, |
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For pulsed lasers: dichroic mirrors |
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Depending on filter cube used |
Internal SP FLIM: 400 … 800 nm |
Depending on filter cube used |
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External FLIM: Depending on filter cube used |
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Internal SP FLIM: 400 … 800 nm |
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External FLIM, FCS, and FLCS: Depending on |
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filter cube used |
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To FCS: yes |
To FLIM: yes |
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UV not required for FCS |
Required for UV FLIM |
To FLCS: yes |
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Alignment stable single pinhole |
Alignment stable single pinhole |
Required for UV FLIM |
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Motorized by software, |
Motorized by software, |
Alignment stable single pinhole |
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automatic mode available |
automatic mode available |
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For FCS |
For FLIM |
For FCS and FLIM |
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Beam park |
Optically correct scanning at low inertia |
FCS: Beam park |
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FLIM: optically correct scanning at low inertia |
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Conventional scanner required |
Conventional and resonant scanner in one |
Conventional scanner required, resonant |
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system (optional) |
scanner optional |
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No scanning during FCS measurement |
For FLIM data acquisition |
For FLIM data acquisition |
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1400 Hz |
1400 Hz |
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1 Hz |
1 Hz |
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1400 |
1400 |
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5 Hz |
5 Hz |
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50 Hz |
50 Hz |
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Yes |
Yes |
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512 x 512 pixel |
512 x 512 pixel |
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1.0 ... 64 x |
1.0 ... 64 x |
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– |
no |
no |
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200° optical |
200° optical |
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22 mm |
22 mm |
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5