Leica NCL-TSH User Manual
Novocastra™ Lyophilized
Mouse Monoclonal Antibody
Thyroid Stimulating Hormone
Product Code: NCL-TSH
Intended Use
Specicity
Clone
Ig Class
Antigen Used for
Immunizations
Hybridoma Partner
Preparation
Effective on Frozen Tissue
Effective on Parafn Wax
Embedded Tissue
Recommendations on Use
Positive Controls
Staining Pattern
Storage and Stability
Warnings and Precautions
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Thyroid stimulating hormone (TSH). No reactivity with luteinising hormone, follicle stimulating
hormone and human chorionic gonadotrophin.
QB2/6
IgG1
TSH molecule.
Mouse myeloma (NS0).
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Yes.
Yes.
Immunohistochemistry on parafn sections.
Enzyme Induced Epitope Retrieval (EIER): Please follow the instructions for use in Novocastra
Enzyme Proteinase K (IHC).
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional ofce of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Immunohistochemistry: Normal human pituitary gland.
Cytoplasmic
Store unopened lyophilized antibody at 2-8 °C. Under these conditions, there is no signicant
loss in product performance up to the expiry date indicated on the vial label. The reconstituted
antibody is stable for at least two months when stored at 2-8 °C. For long term storage, it is
recommended that aliquots of the antibody are frozen at -20 °C (frost-free freezers are not
recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on
the day of use.
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it. This reagent contains sodium azide. A Material
Safety Data Sheet is available upon request or available from www.LeicaBiosystems.com
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( +44 191 215 4242
© Leica Biosystems Newcastle Ltd • NCL- Rev C
TSH 27/07/2012
General Overview
General References
Thyroid stimulating hormone (TSH or thyrotropin) is a glycoprotein produced in the thyrotrophs
of the anterior pituitary gland. TSH and TSH receptor (TSHR) are key proteins in the control of
thyroid function. TSH synthesis in the anterior pituitary is stimulated by thyrotropin-releasing
hormone (TRH) and inhibited by thyroid hormone in a classical endocrine negative-feedback
loop. TSH controls thyroid function upon its interaction with the G protein-coupled TSHR. TSH
binding to its receptor on thyroid cells lead to the stimulation of second messenger pathways
involving predominantly cAMP, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG),
ultimately resulting in the modulation of thyroidal gene expression. TSH also acts as a factor
protecting thyroid cells from apoptosis and plays a critical role in ontogeny.
Fares F A, Levi F, Reznick A Z, et al.. Journal of Biological Chemistry. 276: 4543–4548 (2001).
Oda Y, Sanders J, Evans M, et al.. Thyroid. 10: 1051–1059 (2000).
Haugen B R, Pacini F, Reiners C, et al.. Journal of Clinical Endocrinology and Metabolism. 84:
3877–3885 (1999).
Beck-Peccoz P, Brucker-Davis F, Persani L, et al.. Endocrine Reviews. 17: 610–638 (1996).
Meier C A, Braverman L E, Ebner S A, et al.. Journal of Clinical Endocrinology and Metabolism.
78: 188–196 (1994).
Cole E S, Lee K, Lauziere K, et al.. Biotechnology. 11: 1014–1024 (1993).
www.LeicaBiosystems.com
© Leica Biosystems Newcastle Ltd • NCL- Rev C
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