Leica NCL-L-SP-A User Manual

IVD

Novocastra™ Liquid
Mouse Monoclonal Antibody
Surfactant Protein A

Product Code: NCL-L-SP-A

Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( [phone]

Instructions for Use

Please read before using this product.

Check the integrity of the packaging before use.

www.LeicaBiosystems.com

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Novocastra™ Liquid Mouse Monoclonal Antibody
Surfactant Protein A

Product Code: NCL-L-SP-A

Intended Use

For in vitro diagnostic use.

NCL-L-SP-A is intended for the qualitative identication by light microscopy of human surfactant protein A in parafn sections. The
clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should
be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualied pathologist.

NCL- L- SP-A is recommended for the assessment of surfactant protein A in normal and neoplastic tissue.

Summary and Explanation

The rst immunohistoperoxidase technique was reported by Nakane and Pierce.1 Since then many developments have occurred, leading
to increased sensitivity over earlier techniques. A recent development has been the use of polymeric labeling. This technology has
been applied to both primary antibodies2 and detection systems. The Novolink™ Polymer Detection Systems utilize a novel controlled
polymerization technology to prepare polymeric HRP-linker antibody conjugates. Therefore, the problem of non-specic staining that can
occur with Streptavidin/Biotin detection systems due to endogenous biotin does not occur.

Principle of Procedure

Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specic antibody
to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate
with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The
specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential
diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

Reagent

NCL-L-SP-A is a liquid tissue culture supernatant containing sodium azide as a preservative.

Clone

32E12

Immunogen

Prokaryotic recombinant protein corresponding to the 104–246 amino acid region of the human prosurfactant A molecule.

Specicity

Human surfactant protein A (prosurfactant A).

Ig Class

IgG2a, kappa

Total Protein Concentration

Refer to vial label for lot specic total protein concentration.

Antibody Concentration

Greater than or equal to 35 mg/L as determined by ELISA. Refer to vial label for lot specic Ig concentration.

Warnings and Precautions

This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when

handling it.

This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
Consult federal, state or local regulations for disposal of any potentially toxic components.
Specimens, before and after xation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions.3 Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Minimize microbial contamination of reagents or an increase in non-specic staining may occur.
Incubation times or temperatures, other than those specied, may give erroneous results. Any such changes must be validated by the

user.

Storage and Stability

Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after expiration date indicated on the vial label.
Storage conditions other than those specied above must be veried by the user.

The signs indicating contamination and/or instability of NCL-L-SP-A are: turbidity of the solution, odor development, and presence of
precipitate.

Specimen Preparation

The recommended xative is 10% neutral-buffered formalin for parafn-embedded tissue sections.

Total Protein

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