Leica NCL-L-ALK User Manual
™
Novocastra
Liquid
Mouse Monoclonal Antibody
p80 (Anaplastic Lymphoma Kinase)
Product Code: NCL-L-ALK
Intended Use
Specicity
Clone
Ig Class
Antigen Used for
Immunizations
Hybridoma Partner
Preparation
Effective on Frozen Tissue
Effective on Parafn Wax
Embedded Tissue
Recommendations on Use
Positive Controls
Staining Pattern
Storage and Stability
Warnings and Precautions
General Overview
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( +44 191 215 4242
www.LeicaBiosystems.com
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Human ALK protein and C-terminal ALK fusion proteins containing the immuized target sequence.
5A4
IgG1
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase
catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520 amino acids).
Mouse myeloma (p3-NS1-Ag4-1).
Liquid tissue culture supernatant containing sodium azide.
Volume as indicated on vial label.
Not evaluated
Yes
Immunohistochemistry on parafn sections.
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH 6.
Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional ofce of Leica
Biosystems, or alternatively, visit the Leica Biosystems web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Western Blotting: Not recommended
Immunohistochemistry: a proportion of anaplastic large cell lymphomas.
Cytoplasmic and/or nuclear.
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after
expiration date indicated on the vial label. Storage conditions other than those specied above
must be veried by the user.
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it.
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or
availa ble from www.LeicaBiosystems.com
p80 is encoded by a hybrid gene that occurs as a result of the chromosomal translocation, t(2;5)
(p23;q35). The protein consists of the amino terminus of the nucleophosmin (NPM) gene fused
to the catalytic domain of anaplastic lymphoma kinase (ALK). Full-length ALK is a 1620 amino
acid protein that exhibits 57% amino acid identity to leukocyte tyrosine kinase (LTK). The mature
form of ALK is a 200 kD glycosylated single chain molecule that occurs on the surface of cell
membranes and probably acts as a ligand receptor.
ALK-L 13/12/2012
© Leica Biosystems Newcastle Ltd. • NCL-L Rev D
General References
Liu A, Sugisaki Y, Hosone M, et al. Acta Histochemica et Cytochemica. 2004; 37(1):21-30.
Li X-Q, Hisaoka M, Shi D-R, et al. Human Pathology. 2004; 35:711-721.
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( +44 191 215 4242
www.LeicaBiosystems.com
© Leica Biosystems Newcastle Ltd. • NCL-L Rev D
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