Leica MZ16 F User Manual

Leica MZ16 F

User manual

Leica MZ16 F – User manual 22 Leica MZ16 F – User manual

Dear User,

We thank you for your faith in our product, and
hope it brings you both enjoyment and success.

With the new fully apochromatic Leica MZ16 F
from Leica Microsystems, you have purchased the
best-performing, manual fluorescence stereomi­croscope on the market. The Leica MZ16 F and its
motorized counterpart, the Leica MZ16 FA, provide
the greatest zoom (16:1), the highest resolution (up
to 840 Lp/mm), the highest magnification (115× with
standard optics), the largest numerical aperture
(0.14), a patented illumination and filter system for
the most intense fluorescence, and the innovative
HL RC

TM

high-performance transmitted-light base

for excellent contrast.

The entire imaging system, including the 16:1
zoom, objective and ErgoTube

®

is apochromati­cally corrected using with much technological
effort. Contrast, sharpness, richness in detail,
resolution, image and color fidelity are optimum.
In addition, the patented illumination beam path
guarantees at every zoom level that light utiliza­tion is at a maximum and that fluorescence
images are intensely luminous on a jet black
background.

In developing our instruments, we place great
value on simple, self-explanatory operation. How­ever, please take the time to read the user manual
and the operating safety information so that you
can become familiar with the advantages and fea­tures of your Leica MZ16 F high-performance
stereomicroscope, so that you can use it optimally
and safely.

Should you ever have any questions, please con­sult your local Leica representative. You will find
the address of your nearest local representative,
as well as valuable information about Leica
Microsystems products and services, from
Leica Microsystems on our web site at
www.leica-microsystems.com. We are gladly at
your service. Customer service is a big thing with
us. Not only before the sale, but afterwards as
well.

Leica Microsystems (Switzerland) Ltd.
Stereo & Macroscope Systems
www.stereomicroscopy.com

3 Leica MZ16 F – User manual

Operating controls

1 Zoom magnification changer
2 Coarse/fine focusing drive
3 Double iris diaphragm
4 Rapid filter changer for four selectable filter sets
5 Filter holder for an individually selectable filter
6 Switch for activating/deactivating the

UV shutter

7 Trinocular video/phototube
8 Adjustable tubes for adjusting the

interpupillary distance from 52mm–76mm

9 Knurled rings for adjusting the diopter settings

from +5 to –5

10 Adjustable and attachable eyecups

7
6

4

3

5

1

2

10

9

8

Fig. 1 Leica MZ16 F with trinocular video/phototube and Leica digital camera

Page

4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1.1 Fluorescence technology . . . . . . . . . . .18

4.1.2 Functional principle . . . . . . . . . . . . . . . .18

5. Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

5.1 Stereomicroscope settings

and adjustments . . . . . . . . . . . . . . . . . . . . . . . .18

5.1.1 Observation without fluorescence . . . .19

5.1.2 Double iris diaphragm . . . . . . . . . . . . . .19

5.2 Commissioning the fluorescence system . .20

5.3 Rapid filter changer . . . . . . . . . . . . . . . . . . . . .20

6. Work flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

7. Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

7.1 Filter sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

7.2 Technical data . . . . . . . . . . . . . . . . . . . . . . . . .23

7.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . .27

7.4 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

Page

1. Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . .5

1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . .5

1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . .5

1.1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . .6

1.2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . .6

1.3 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

2. Overview of the instrument . . . . . . . . . . . . . .10

3. Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

3.1 Assembly sequence . . . . . . . . . . . . . . . . . . . .12

3.1.1 Expansion diagram . . . . . . . . . . . . . . . . .13

3.2

Assembly of stereomicroscope with stand

. .14

3.3 Additional components . . . . . . . . . . . . . . . . . .15

3.3.1 Video/phototube . . . . . . . . . . . . . . . . . . .15

3.4 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

3.4.1 Lamp housing 106 z . . . . . . . . . . . . . . . . .15

3.4.2 Supply units . . . . . . . . . . . . . . . . . . . . . . .15

3.5 UV protection screen . . . . . . . . . . . . . . . . . . . .16

3.6 Equipping the filter changer . . . . . . . . . . . . . .16

3.6.1 Filter sets for fluorescence . . . . . . . . . .16

3.6.2 Simple filter holders . . . . . . . . . . . . . . . .17

3.6.3 Inserting the filter . . . . . . . . . . . . . . . . . .17

3.6.4 Slot for filter slide . . . . . . . . . . . . . . . . . .17

4 Leica MZ16 F – User manual

Contents

Leica MZ16 F – User manual 5Leica MZ16 F – Safety concept

5

1. Safety concept

1.1 The user manual

Included with your Leica MZ16 F fluorescence
stereomicroscope is an interactive CD-ROM with
all pertinent user instructions in German, English,
French, Spanish, Italian, Portuguese, Dutch, Dan­ish, Swedish, Finnish and Greek. Keep it in a safe
place, and readily accessible to the user. User
manuals and updates are also available for you to
download and print from our web site at
www.stereomicroscopy.com.

The Leica MZ16 F is the manual fluorescence
stereomicroscope of the Leica M stereomicro­scope series. This operating manual describes
the special functions of the Leica MZ16 F and
important instructions for its operational safety,
maintenance, and accessories. Like all instru­ments of the Leica M stereomicroscope series,
the Leica MZ16 F consists of modules, which
allow an almost unlimited variety of custom
equipment configurations. The identical modules,
such as stands, binocular tubes, accessories, etc.
are described in User Manual M2-105-0 for
Leica M stereomicroscopes, which also contains
additional safety instructions pertaining to the
stereomicroscope, accessories and electrical
accessories, as well as care instructions.

Special manuals are provided for a number of
accessories:
– User Manual M2-216-1 for lamp housing with

high-pressure mercury burner.

– User Manual for ebq 100 isolated

supply unit for 100W high-pressure
mercury burners.

– User Manual M2-267-1

for the motorized focus system.

– User Manual M2-166-2 for

FluoCombi III

TM

Before installing, operating or using the
instruments, read the user manuals listed above.
In particular, please follow all safety instructions.

To maintain the unit in its original condition and to
ensure safe operation, the user must follow the
instructions and warnings contained in these user
manuals.

1.1.1 Diagrams

(1.2) Numbers in parentheses within the descrip­tions refer to diagrams and the items in these
diagrams. Example (1.2):
Figure 1 is located on page 3, and item 2 is the
focusing drive.

6 Leica MZ16 F – Safety concept

1.1.2 Symbols used

Warning of a danger

This symbol indicates especially important infor­mation that, if not observed,
– can cause hazards to personnel,
– can lead to functional disturbances and dam-

aged instruments.

Warning of hazardous electrical voltage

This symbol indicates especially important infor­mation that, if not observed,
– can cause hazards to personnel,
– can lead to functional disturbances and

damaged instruments.

Danger due to hot surface

This symbol warns against touching hot surfaces,
e.g. those of light bulbs.

Important information

This symbol indicates additional information or
explanations that intend to provide clarity.

Action

왘 This symbol refers to actions described in the

text that are to be carried out.

Additional notes

• This symbol indicates additional notes and
explanations provided in the text.

1.2 Safety instructions

Description

The Leica MZ16 F is a manual fluorescence stere­omicroscope with 16:1 zoom for fluorescence
applications. The optics system consists of
two observation beam paths, a patented Triple­Beam

®

third beam path specifically for fluores-

cence illumination and the patented FLUOIII

®

filter
system. The entire imaging system, including the
16:1 zoom, objective and ErgoTube

®

is apochro-

matically corrected.

The Leica MZ16 F consists of these modules:
– Stereomicroscope
– Stand, binocular tube, objective, eyepieces and

(if applicable) accessories for photography,

etc.
– Power supply
– Filter sets with barrier and excitation filters on

one filter holder
– Three simple filter holders for transmitted light

observation and protection from UV light
– Filter holder for individual filters
– UV protection screen
– 106 z lamp housing with 50W or 100W high-

pressure mercury burner with stray-light

protection
– Supply unit

Leica MZ16 F – User manual 7Leica MZ16 F – Safety concept

Permitted uses

With the Leica MZ16 F fluorescence stereomicro­scope, entire fluorescent specimens can be
examined three-dimensionally and unprepared,
manipulated, sorted and documented. The sepa­rate beam path for the fluorescence illumination
in the Leica MZ16 F as well as the very bright
high-pressure mercury burner and specially
selected filter sets enable you to distinguish the
finest structures and enhance the quantity of
information for incident light fluorescence. Spe­cial filter holders also enable observation without
fluorescence in transmitted light or incident light.

Non-intended use

If the Leica MZ16 F, its components and acces­sories are used other than as described in the
user manual, injury or property damage may
occur.
Never:
– install other plugs or cables.
– change, rebuild or take apart parts, if not

specifically instructed to in this manual.
– allow unauthorized persons to open parts.
– use the MZ16 F for examining, or operating on

the human eye.

The devices and accessories described in this
User manual have been tested for safety and
potential hazards. The responsible Leica affiliate
or the main plant in Wetzlar must be consulted
whenever the device is altered, modified or used
in conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the device or noncom­pliant use shall void all rights to any warranty
claims!

Place of use

– The Leica MZ16 F may only be operated in a

closed, dust-free room, which is free of oil
vapors and other chemical vapors, as well as
extreme humidity, and within a temperature
range of +10 to +40.

– Electrical components must be assembled at

least 10 cm away from the wall and from flam­mable substances.

– Large temperature fluctuations, direct sunlight

and vibrations should be avoided. These condi­tions can distort measurements and micro­graphic images.

– Stereomicroscopes in warm and warm-damp

climatic zones require special care in order to
prevent the build up of fungus.

Responsibilities of the person(s) in charge of
instrument

Ensure that
– the Leica MZ16 F and accessories are oper-

ated, maintained and repaired only by autho­rized and trained personnel.

– the operating personnel has read, understands,

and is observing these instructions as well as
those listed on page 5, and, in particular, the
safety instructions.

Transport

– Use the original packaging for shipping or

transporting the stereomicroscope and its
accessory components.

– In order to prevent damage from vibrations,

disassemble all moving parts that (according to
the user manual) can be assembled by the cus­tomer and pack them separately. These include
the objective, binocular tube, eyepieces, lamp
housing, burner in the 106 z lamp housing, etc.

8 Leica MZ16 F – Safety concept

Repairs and servicing

– Only Leica Microsystems-trained service tech-

nicians, or technical specialists assigned by

the person in charge of the instrument, are

permitted to carry out repairs.
– Only original Leica Microsystems spare parts

may be used.
– Disconnect the power cable before opening

voltage-carrying parts such as the power

supply or the supply unit.

Touching the live circuit can cause injury.

Installation in third-party products

When installing Leica products into third-party
products, note the following:
The manufacturer of the complete system or its
dealer is responsible for following all applicable
safety instructions, laws and guidelines.

Disposal

– The product must be disposed of in accordance

with locally applicable laws and regulations.
– Dispose of used high-pressure mercury burn-

ers as hazardous waste.

Legal requirements

Adhere to general and local regulations relating to
accident prevention and environmental protec­tion.

Conformity with European Community directive

The Leica MZ16 F and its accessories are con­structed in accordance with the latest technolo­gies and are provided with a statement of confor­mity with EC requirements.

Health risks

Workplaces with stereomicroscopes both
improve and make visual tasks easier, but they
also place high demands on the human visual
apparatus and the muscular "holding apparatus"
of the user. Depending on the duration of uninter­rupted work, asthenopia and musculoskeletal
problems may occur. For this reason, appropriate
measures for reduction of the workload must be
taken:
– optimal arrangement of workplace, work

assignments and work flow (changing tasks
frequently).

– thorough training of the personnel, giving

consideration to ergonomic and organization
aspects.

The ergonomic design and construction of
Leica M series stereomicroscopes are intended
to reduce the exertion of the user to a minimum.

Direct contact with eyepieces is a potential
transmission method for bacterial and viral infec­tions of the eye.
The risk can be kept to a minimum by using per­sonal eyepieces for each individual or detachable
eyecups.

Leica MZ16 F – Safety concept 9

1.3 Light source: safety instructions

Precautions by the manufacturer

– UV protection screen in front of the specimen

plane prevents the user from looking directly
into the UV rays.

–

Simple filter holders (that have only two open­ings for the observation beam paths) in the avail­able positions of the rapid filter changer prevent
direct UV radiation from reaching the eyes.

– UV shutter (which can be activated/deacti-

vated) prevents direct UV radiation from reach­ing the eyes.

– UV filters in the observation beam path protect

the eyes.

– The stray-light protection on the lamp housing

prevents irradiation of the hands.

Warning

UV radiation can harm the eyes.

Therefore:
– Never look into the light spot on the specimen

plane without a UV protection screen.

– Always fill all of the filter positions with filter

holders.

– Fill empty filter positions with the simple filter

holders (which have only two openings for the
observation beam paths).

– Do not select a white, strongly reflective

background for the stage.

– Never open the lamp housing while the lamp is

switched on.

Risk of explosion, UV exposure, blinding!

Supply unit

Always unplug the supply unit from the power
supply:
– when installing and disassembling the lamp

housing
– before opening the lamp housing
– when replacing the high-pressure mercury

burner and other parts, such as the heat-

absorbing filter or the collector
– during maintenance work on the supply unit.

Lamp housing

The Leica MZ16 F may be used only together with
the Leica 106 z lamp housing for the 100W or 50W
mercury burner, supply unit and stray-light protec­tion.

Leica Microsystems accepts no liability for
damages resulting from the use of lamp housings
from other manufacturers.

– Never open the lamp housing while the lamp is

switched on. Unplug the supply unit from the
power supply:

Risk of explosion, UV exposure, blinding!

– Before opening the lamp housing, allow it to

cool off for at least 15 minutes.

Danger of explosion, danger of burns.

– Never cover the air duct on the lamp housing.

Danger of fire!

– Use the stray-light protection.

Danger to eyes from UV radiation.