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Leica
Application
Suite
Multifocus
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Brilliant Depth of Focus!
Automatic Image Acquisition
The LAS Multifocus module is designed
to acquire extended depth of field images
from Leica motorised microscopes. Exposure, gain, shading and all other camera
parameters can be individually set to optimise the quality of image acquisition.
Other benefits include:
• Comprehensive digital camera control
functions including the ability to adjust
focus position on live image display.
• Fully integrated control of motorised
focus on Leica Stereomicroscopes,
Leica Macroscopes and Leica
Compound microscopes, both upright
and inverted.
• Simplified operations by automatically
adjusting step size and the number
of images to microscope magnification,
aperture and camera resolution.
• Automatic Z-stack image capture
for routine operation. Different
configurations can be stored for
later recall, allowing immediate use
with standard specimens.
• Manual operation for
when motorised focus is
not available.
Create Multifocus
Once digital images have been collected at
different Z-positions they are intelligently
combined into one single sharp composite
image that massively extends the depth
of focus.
• Further enhancements can be applied
such as contrast, brightness and gamma
adjustment to optimise image display.
• A small region of interest may be selected
from the whole image to identify an
area of significance and view it in detail.
• Images from microscopes with nontelecentric optics can be resized and
aligned using auto correlation.
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Magnificent Visualisation!
Annotation
The annotation facilities make the LAS
Multifocus Module a perfect solution for
creating data rich presentations and reports:
• Images can be annotated with calibration
markers for a visual guide to image size.
• Further annotations can be added such
as the image name, date of acquisition
and description.
• Annotations can be merged into an image
for a permanent record.
LAS is based on Windows PCs and provides
a cost-effective and uniform environment,
compatible across the Leica range of
microscopes and cameras. Furthermore,
images may be exported for additional
processing.
Visualisation
A gallery of the Z-Stack is available from
which individual images can be immediately
selected and displayed in the main image
window. This means that specific images
can be located and viewed quickly and easily.
Other features include:
• An image display which adapts automatically to the resolution of the acquired
or imported image including the ultra
high resolutions provided by Leica Digital
FireWire Cameras.
• Zoom and pan so that images can be
viewed in detail.
• Images can be saved to a named folder
that contains the entire Z-stack, the
Multifocus image and configuration
details, making it an easy task to reload
with the same conditions.
Monitor Image: Detail from circuit
board taken with a Leica Z16 APO A
motorized macroscope with a
Leica digital camera
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The Leica Application Suite Multifocus
module provides:
• A workflow orientated User Interface with
simple interactive means for defining the
Z range to scan.
• Total microscope and digital camera control
in a fully integrated manner.
• Automatic acquisition of extended depth
of
field images from Leica motorised
microscopes.
•
A gallery of Z-Stack images directly visible so
that focus changes can be easily visualised.
•
Alignment of images from microscopes where
images are displaced by focus position.
@
www.leica-microsystems.com
LAS Multifocus
The Leica Application Suite Multifocus module easily acquires extended depth of field images by providing fully
integrated control of microscopes with motorised focus. The automatic acquisition of Z-Stack images, combined with
intelligent image combination algorithms, makes the capture and storage of sharply focused images an undemanding
process. As use is automated it means that there is minimal need for operator intervention however, settings can be
easily modified to suit a wide range of sample types in fields such as material science, forensics, bioscience, and
earth sciences.
Order number
12730064 Leica Application Suite Multifocus Module
Leica Microsystems (Switzerland) Ltd
Stereo & Macroscope Systems
CH-9435 Heerbrugg
Telephone +41 71 726 33 33
Fax +41 71 726 33 99
www.leica-microsystems.com
www.stereomicroscopy.com
Illustrations, descriptions and technical data are not binding and may be changed without notice.
Printed on chlorine-free paper with a high content of recycled fibre.
M1-525-2en • © Leica Microsystems (Switzerland) Ltd • CH-9435 Heerbrugg, 2005 • Printed in Switzerland – VIII.2005 – RDV
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