Leica LAS ANALYSIS, LAS IMAGE, APPLICATION SUITE IMAGE User Manual

LAS Image Analysis

Automatic Analysis of Multiple Image Features

Meaningful Results

LAS Image Analysis is a sophisticated software module that
automatically performs feature detection, measurement and the
evaluation of multiple image features. In conjunction with the
Leica digital camera and microscope control, LAS can be used in
a diverse range of imaging fields. With it’s Sequence Panel, LAS
Image Analysis guides you through the set up process to simplify
the procedure of acquiring meaningful data and once established,
these settings can be used repetitively for future analyses.

LAS provides you with all you need to acquire a high quality
image of a specimen. Images can be acquired individually or
by automatically scanning a specimen using the optional LAS
MultiStep module. Images can then be processed by LAS Image
Analysis to enhance the edges of features so that they are
accurately measured. By simply using the thresholding functions,
you can specify the minimum and maximum gray scale values or
the hue saturation and intensity levels of a colour image to identify
the required image detail, prior to measurement.

If some minor debris is still visible after thresholding, then binary
processing may be employed to improve the image. A selection of
binary processing operations are provided and these can be tested
interactively to ensure that the image is optimised appropriately.
Once you have selected a method that is suitable, the conditions
can be stored for use with further images. LAS Image Analysis
also provides manual ‘image editing’ for the occasions when a
more hands on approach is preferable. This allows you to indicate
regions suitable for analysis or to identify the features to be
measured and remain in complete control of the specimen.

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Effortless Feature Measurements

Once the binary image has been produced, the features in
this image can be measured to give a variety of morphological
information such as size, shape and position. The large number
of parameters available gives you the option of selecting the
information that is most appropriate to your sample to give you a
meaningful characterisation of your specimen.

When the measurement has been completed, the resulting data
is stored for review, summary, and interpretation. Raw data can
be examined and statistics calculated in order that trends can
be identified, whilst histograms and pie charts can be plotted to
further organise the specimen information. Result data can then
be outputed to a Microsoft Excel template so that customised
reports can be created and further calculations derived.

Key benefits:

An easy to use sequence control that •
guides you through the whole set up
process for acquisition, detection and
measurement.

Save and recall settings and •
configurations to exactly recreate the
same conditions at a later date

Sophisticated measurements on •
individual features including size, shape,
position, orientation and intensity

Create meaningful results with a range •
of analysis tools inlcuding statistics,
histograms and pie charts

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“With the user, for the user”
Leica Microsystems

Leica Microsystems operates internationally in four divi­sions, where we rank with the market leaders.

Life Science Division

•

The Leica Microsystems Life Science Division supports the
imaging needs of the scientific community with advanced
innovation and technical expertise for the visualization,
measurement, and analysis of microstructures. Our strong
focus on understanding scientific applications puts Leica
Microsystems’ customers at the leading edge of science.

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•

The Leica Microsystems Industry Division’s focus is to
support customers’ pursuit of the highest quality end result.
Leica Microsystems provide the best and most innovative
imaging systems to see, measure, and analyze the micro­structures in routine and research industrial applications,
materials science, quality control, forensic science inves­tigation, and educational applications.

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•

The Leica Microsystems Biosystems Division brings his­topathology labs and researchers the highest-quality,
most comprehensive product range. From patient to pa­thologist, the range includes the ideal product for each
histology step and high-productivity workflow solutions
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Leica Microsystems creates better patient care through
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•

The Leica Microsystems Surgical Division’s focus is to
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microscope technology today and into the future.

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