Leica IN VITRO FERTILIZATION User Manual

In Vitro Fertilization

Your Applications – Our Solutions

Systems and Instruments for the IVF Lab

Intracytoplasmic Sperm Injection (ICSI) has become a standard

method in IVF labs around the globe. The right equipment in your

lab supports your effort to constitute a good laboratory practice,

to work in a convenient and effective way and at the end to in-

crease the reproduction rate.

The microscopes and accessories of Leica Microsystems are

designed to support your daily work.

The Leica Microsystems inverted microscopes are especially

appreciated for their superior optical performance in imaging

oocytes, sperms and injection capillary. Stability and robust-

ness are matters of course to provide you with best tools for

your work.

Focus on the Oocyte

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At a glance:

Free of vibrations – stable stand to carry •

all common types of micromanipulators

Freedom of choice – manual and auto-•

mated components, such as stage,

condenser, objectives

Superior optics for brilliant images –•

unique Leica integrated modulation

contrast for standard objectives 5x–63x

Convenient handling of the petri dish at •

the right temperature – 37°C heating stage

with fl at surface and glass bottom

Compatibility – further supporting•

components for daily routine or

sophisticated research

The perfect ICSI workstation: Leica DMI3000 B, 3-plate stage,condenser S40 for modulation contrast

for objectives 5x to 63x, new Narishige manipulators with injection units, heating stage 37°C.

Courtesy of: C. Mehnert, Zentrum für In vivo Fertilization,

Giessen, Germany

The Tokaihit MATS, heating plate becomes fl ush with the

stage surface to ensure easy handling of the specimens

and easy operation of the manipulator

The method of intracytoplasmic morphologically selected sperm

injection (IMSI) has gained a lot of interest in the last years. In

close collaboration, listening to the voice of our users, Leica

Microsystems has set up a sophisticated and convenient working

station for IMSI.

The Leica Microsystems solution provides superior optical perform-

ance combined with ease of use: The unique micromanipulation

system Leica AM6000 combined with IMSI allows the control of

the micromanipulator and microscope by one control panel and

offers highest performance for IMSI and ICSI on one workstation.

The 100x objective with high numerical aperture together with

additional optical magnifi cation provides a total magnifi cation of

up to 16000x on an LCD screen to clearly identify and analyze the

morphology of the sperm.

Zoom in on the Sperm

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The perfect combined IMSI and ICSI workstation: unique Leica AM6000, motorized 3-plate stage,

DIC or modulation contrast for objectives 5x up to 100x, heating stage 37°C.

At a glance:

Manipulator and microscope connected•

via digital interface – manipulator move-

ment is aligned to magnifi cation

Less vibration – one control panel for •

microscope and manipulators

Reproducible images – all system•

functions are intelligently automated

Only one company to deal with –•

one supplier of electronics, optics and

mechanical components

Ergonomic benefi ts and operating safety – •

by integral control and top level motorization

The optical zoom system accomplishes a continuous

view of the moving sperms, while selecting the optimal

magnifi cation. You can keep the orientation to the edge

of the drop of medium.

Superior DIC – Best contrast at the push of a button.

Depending on the user preferences the Leica Integrated

Modulation Contrast is also available.