Leica IN VITRO FERTILIZATION User Manual

In Vitro Fertilization

Your Applications – Our Solutions

Systems and Instruments for the IVF Lab

Focus on the Oocyte

At a glance:

Free of vibrations – stable stand to carry •
all common types of micromanipulators

Freedom of choice – manual and auto-•
mated components, such as stage,
condenser, objectives

Superior optics for brilliant images –•
unique Leica integrated modulation
contrast for standard objectives 5x–63x

Convenient handling of the petri dish at •
the right temperature – 37°C heating stage
with fl at surface and glass bottom

Compatibility – further supporting•
components for daily routine or
sophisticated research

Intracytoplasmic Sperm Injection (ICSI) has become a standard
method in IVF labs around the globe. The right equipment in your
lab supports your effort to constitute a good laboratory practice,
to work in a convenient and effective way and at the end to in­crease the reproduction rate.

The microscopes and accessories of Leica Microsystems are
designed to support your daily work.

The Leica Microsystems inverted microscopes are especially
appreciated for their superior optical performance in imaging
oocytes, sperms and injection capillary. Stability and robust­ness are matters of course to provide you with best tools for
your work.

Courtesy of: C. Mehnert, Zentrum für In vivo Fertilization,
Giessen, Germany

The Tokaihit MATS, heating plate becomes fl ush with the
stage surface to ensure easy handling of the specimens
and easy operation of the manipulator

2

The perfect ICSI workstation: Leica DMI3000 B, 3-plate stage,condenser S40 for modulation contrast
for objectives 5x to 63x, new Narishige manipulators with injection units, heating stage 37°C.

Zoom in on the Sperm

The method of intracytoplasmic morphologically selected sperm
injection (IMSI) has gained a lot of interest in the last years. In
close collaboration, listening to the voice of our users, Leica
Microsystems has set up a sophisticated and convenient working
station for IMSI.

The Leica Microsystems solution provides superior optical perform­ance combined with ease of use: The unique micromanipulation
system Leica AM6000 combined with IMSI allows the control of
the micromanipulator and microscope by one control panel and
offers highest performance for IMSI and ICSI on one workstation.
The 100x objective with high numerical aperture together with
additional optical magnifi cation provides a total magnifi cation of
up to 16000x on an LCD screen to clearly identify and analyze the
morphology of the sperm.

At a glance:

Manipulator and microscope connected•
via digital interface – manipulator move­ment is aligned to magnifi cation

Less vibration – one control panel for •
microscope and manipulators

Reproducible images – all system•
functions are intelligently automated

Only one company to deal with –•
one supplier of electronics, optics and
mechanical components

Ergonomic benefi ts and operating safety – •
by integral control and top level motorization

The perfect combined IMSI and ICSI workstation: unique Leica AM6000, motorized 3-plate stage,
DIC or modulation contrast for objectives 5x up to 100x, heating stage 37°C.

Superior DIC – Best contrast at the push of a button.
Depending on the user preferences the Leica Integrated
Modulation Contrast is also available.

The optical zoom system accomplishes a continuous
view of the moving sperms, while selecting the optimal
magnifi cation. You can keep the orientation to the edge
of the drop of medium.

3

The Spermogram

At a glance:

Stability and robustness, combined with •
highest optical performance – complete
range of routine phase contrast micro­scopes Leica DM1000 to DM3000

Long working distance objectives –•
10x, 20x, 40x, 63x to allow work with
counting chambers

Study the fi
up to 100x oil objective

Heating stage 37°C available•

Ergonomical, fatigue-free working – •
height adjustable focus knobs and other
equipment

ne morphology of the sperm –•

The fi rst step to assisted reproduction is to check the quality of
spermatozoa, via motility, morphology, composition of the ejacu­late and other biochemical parameters.

Leica Microsystems’ contribution to this step are high quality, er­gonomic upright microscopes, equipped with 37°C heating stage
to support your work the best way. The phase contrast visualizes
the sperm head as a small white sphere, while the fl agellum shows
up as a dark fi lament. Leucocytes present within the ejaculate can
be easily identifi ed.

A full range of ergonomic equipment like tilting tubes, height ad­justable focus knobs, and ergonomical stages make work more
comfortable, pleasant, and fatigue-free.

MAKLER Counting Chamber for rapid sperm analysis per­fectly fi ts the Leica DM1000-3000 stages for convenient
evaluation.

4

Ideal solution for sperm analysis: the unique ergonomic system microscopes
Leica DM1000, DM2000, DM2500 and DM3000 with heating stages 37°C.

Decoronization and
Zygote Observation

After the collection of the oocytes via follicle puncture, the oo­cytes must be stripped of their granulosa cells. This delicate and
important work requires excellent stereoscopic, 3D visualization
combined with a long working distance to offer easy and conve­nient access for the tools of the operator.

After fecundation, the zygotes are observed to check the number,
size and location of the pronuclei. The stereomicroscope is one
of the optical tools used for visualization of the development from
the zygote to the blastocyst state. Highest image quality combined
with ergonomic design, ease of use and reliability are crucial for
this essential step within assisted reproduction.

The new Leica stereomicroscopes are available in manifold con­fi gurations offering highest modularity and accessories. The new
Leica FusionOptics™ has again exceeded the limits in resolution
and depth of fi eld.

The oocyte in stereo:

Brilliant image quality•

Perfect stereo impression also•
with spectacles

High working distance up to 135 mm•

Zoom up to 160x (1x objective)•

Stable and vibration free design•

Manifold accessories, heating stage, •
camera, software

Highly effective instruments for manual inspection:

Leica M125, M165 C, M205 C modular stereomicroscopes.

Human oocyte before decoronization

Human oocyte after decoronization

Courtesy of: Pr.S. Viville, Dr. C.Wittemer CMCO-SIHCUS
Schiltigheim (France)

5

Advanced Analysis
Tools for Cytogenetics

Leica CW4000 CytoFISH at a glance

Acquisition facilities for up to 16 image •
planes, allowing the capture of standard
FISH, MFISH and CGH images

Autoexposure facility can be used to •
provide a ‘one touch’ fully automatic
capture sequence

Capture a region of interest in the camera •
fi eld of view, or capture using the entire
CCD array for the highest spatial resolu­tion. Binning can optionally be used to
increase sensitivity

No need to threshold images to generate •
composite images – this removes the
possibility of excluding faint ‘real’ signals
from the image

Full compatibility with karyotyping •
applications to allow fl exible karyotyping
of 2, 3 or multicolor images

The Leica CW4000 CytoFISH provides fl uorescence image acqui­sition and advanced analysis tool for prenatal diagnostics (PGD).
It includes the ability to count FISH signals in interphase cells and
to perform a ratio of the number of two different color FISH spots.
The Leica CW4000 software is fully compatible with the Leica digi­tal microscopes DM4000–6000 B and Leica FireWire cameras. The
user can combine brightfi eld and fl uorescence images to view
CISH or FISH images and combine immunostaining with FISH ex­periments.

Leica CW4000 CytoFISH offers a range of easy to use tools to ad­just contrast, color and defi nition of objects in the acquired im­ages. The modular system includes a full range of Karyotyping,
FISH, CGH and MFISH options, providing outstanding accurate,
detailed and high quality images and data.

Different DNA areas on different chromosomes are
marked using fl uorescent probes. Courtesy of: Guangzhou
No.2 Ren Min Hospital – O&G Institute; X. F. Sun

6

The intelligent automated system for cytogenetics:

Leica DM6000 B and Leica CW4000 CytoFISH.

Open and Compatible
with Additional Equipment

The Leica Microsystems portfolio covers a wide range of prod­ucts useful within your IVF laboratory for routine and more sophis­ticated applications and related research applications. Beside
inverted, upright and stereomicroscopes a wide range of digital
cameras are available, offering highest resolution and highest
sensitivity.

Digital Leica cameras and comprehensive software packages
are available for digital imaging using single shots or movie se­quences.

Leica Microsystems is in close contact with
other suppliers for IVF to continuously opti­mize our offering for your needs:

Zona pelucida laser drilling•

Sterile clean benches•

Consumables (capillaries, dishes,•
counting chambers, ...)

Manipulators and further equipment •
around IVF

Leica LAS Application software modules:

Image database, measurement and analysis.

The best combination for zona pelucida drilling – Leica
DMI3000–6000 B and the Hamilton Thorne ZILOS-tk® laser

Cleanbench with Leica AM6000 workstation for IVF

7

“With the user, for the user”
Leica Microsystems

Leica Microsystems operates globally in four divi sions,
where we rank with the market leaders.

Life Science Division

•

The Leica Microsystems Life Science Division supports the
imaging needs of the scientifi c community with advanced
innovation and technical expertise for the visualization,
measurement, and analysis of microstructures. Our strong
focus on understanding scientifi c applications puts Leica
Microsystems’ customers at the leading edge of science.

Industry Division

•

The Leica Microsystems Industry Division’s focus is to
support customers’ pursuit of the highest quality end result.
Leica Microsystems provide the best and most innovative
imaging systems to see, measure, and analyze the micro­structures in routine and research industrial applications,
materials science, quality control, forensic science inves­tigation, and educational applications.

Biosystems Division

•

The Leica Microsystems Biosystems Division brings his­topathology labs and researchers the highest-quality,
most comprehensive product range. From patient to pa­thologist, the range includes the ideal product for each
histology step and high-productivity workfl ow solutions
for the entire lab. With complete histology systems fea­turing innovative automation and Novocastra™ reagents,
Leica Microsystems creates better patient care through
rapid turnaround, diagnostic confi dence, and close cus­tomer collaboration.

Surgical Division

•

The Leica Microsystems Surgical Division’s focus is to
partner with and support surgeons and their care of pa­tients with the highest-quality, most innovative surgi cal
microscope technology today and into the future.

The statement by Ernst Leitz in 1907, “with the user, for the user,” describes the fruitful collaboration
with end users and driving force of innovation at Leica Microsystems. We have developed fi ve
brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to
Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.

Active worldwide

Australia: North Ryde Tel. [phone] Fax [phone]
Austria: Vienna Tel. [phone] Fax [phone]
Belgium: Groot Bijgaarden Tel. [phone] Fax [phone]
Canada: Richmond Hill/Ontario Tel. [phone] Fax [phone]
Denmark: Herlev Tel. [phone] Fax [phone]
France: Nanterre Cedex Tel. [phone] Fax [phone]
Germany: Wetzlar Tel. [phone] Fax [phone]
Italy: Milan Tel. [phone] Fax [phone]
Japan: Tokyo Tel. [phone] Fax [phone]
Korea: Seoul Tel. [phone] Fax [phone]
Netherlands: Rijswijk Tel. [phone] Fax [phone]
People’s Rep. of China: Hong Kong Tel. [phone] Fax [phone]
Portugal: Lisbon Tel. [phone] Fax [phone]
Singapore Tel. [phone] Fax [phone]
Spain: Barcelona Tel. [phone] Fax [phone]
Sweden: Kista Tel. [phone] Fax [phone]
Switzerland: Heerbrugg Tel. [phone] Fax [phone]
United Kingdom: Milton Keynes Tel. [phone] Fax [phone]
USA: Bannockburn/lllinois Tel. [phone] Fax [phone]

and representatives in more than 100 countries

Order no.: English 914 693 • VI/09/DX/BR.H.

LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

www.leica-microsystems.com