
Measuring Up with LAS!
Interactive Measurements
The Leica Application Suite Interactive
Measurement Module is designed to
simplify the manual tasks involved when
deriving calibrated measurement parameters
such as length, area, perimeter,
diameter and angles. By tracing around an
object of interest, it is easy to identify areas
of significance and perform sophisticated
measurements upon them. The colour and
line thickness of measurement tracings
can even be adjusted to contrast fully with
the underlying image or merged into it to
create a permanent record. Furthermore,
each measurement can be labelled with a
sequence number, a parameter name, a
value and a written comment.
The LAS Interactive Measurement module
allows objects to be counted individually
and assigned to a labelled class which
can be allocated a specific colour for
identification purposes. Additionally, those
that consist of multiple fragments can be
grouped so that the total area of the group is
derived. All configurations defined during
measurements can also be saved and
recalled for convenient repetition.
Other benefits include:
• Fully integrated with microscope and
digitial camera control.
•
Measurements can be applied to
individual
images or accumulated over
multiple images so that statistical
trends can be determined.
• Live images can be annotated with
calibration markers to provide a quick
and easy guide to image size.
• Object tracings can be stored and
recalled in overlay format so that they
can be reviewed effortlessly.
• An intuitive gallery displays acquired
images, which can be immediately
selected for measurements.

Vital Statistics!
Detailed Results
In order to provide the most in depth
findings the LAS Interactive Measurements
module provides various options for reviewing and reporting results. Measurement
results can be displayed in tabular form
and easily saved, exported and printed in
Microsoft Word and Excel. Furthermore,
statistics can be calculated in terms of total,
mean, mode, median, standard deviation,
maximum and minimum values and many
more.
LAS is based on Windows PCs and provides
a cost-effective and uniform environment,
compatible across the Leica range of
microscopes and Digital FireWire cameras.
Measurement Options
The LAS Interactive Measurement module
provides a workflow approach to defining
the parameters during setup, through to the
actual measurements and the subsequent
analyses. Measurement types include
depicting the vector distance between
points, determining angle, width and height
and calculating areas and mean intensity.
The meaurement process can be operated
in two modes, either in basic or accumalation. Basic mode is mostly applied to
measurements on individual images whilst
accumalation mode can be used when
calculations are applied to multiple images
to obtain statistical information.

•
Layer Thickness Measurements –
cross sectional
samples are measured to
discover coating thickness and skin thickness.
• Cell Area Measurements
–
the circumference of a cell is traced. Where
several cells have been traced, the area
average can be determined. All tracings can
be saved and recalled for any future editing
requirements.
• Count Tool
–
The number of positive and negetive cells can
be counted in an immunological stain and
appropriate classes assigned. Counts can be
accumalted for each class and labelled
appropriately.
LAS Interactive Measurements
The LAS Interactive Measurement module is a highly versatile solution that can be used with many specimen types
for users interested in materials science, quality control, forensics, bioscience, pharmacology and many more areas.
The LAS Interactive Measurement module can be used for applications such as:
Order number
12730072 Leica Application Suite – Interactive Measurement Module
Illustrations, descriptions and technical data are not binding and may be changed without notice.
Printed on chlorine-free paper with a high content of recycled fibre.
M1-525-6en • © Leica Microsystems (Switzerland) Ltd • CH-9435 Heerbrugg, 2005 • Printed in Switzerland – II.2006 – RDV
www.leica-microsystems.com/LAS