Lab-Line 314, 314-1, 314-1CE, 316, 316-1 User manual

1/01

OPERATION MANUAL

MANUAL NO. 057-561-00

REV. A

LAB-LINE®

AIR-JACKETED COMPACT

AUTOMATIC & FLO-THRU®

CO2 INCUBATORS

MODEL NO. AUTOMATIC:

314, 314-1, 314-1CE (Stainless Steel Interiors)

316, 316-1 (Copper Interiors)

FLO-THRU: 315, 315-1

(Stainless Steel Interiors)

317, 317-1 Copper Interiors)

DESIGNERS AND MANUFACTURERS

A SUBSIDIARY of Barnstead|Thermolyne

1999 North 15th Ave., Melrose Park, IL 60160-1491 USA PHONE: [phone] or [phone]; FAX: [phone]

TABLE OF CONTENTS

2

SECTION TITLE

1Introduction

2Description

3Specifications

4Features

5Installation

6Operation

7Maintenance

8Replacement Parts Warranty

3

BE ADVISED:

IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION INSTRUCTIONS EXACTLY AS WRITTEN—FAILURE TO DO SO IS LIKELY TO LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION.

THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER:

•Dry CO2 gas (research grade or better).

•A dual-stage regulator for the CO2 tank.

•¼" ID Flexible tubing (appropriate length from tank to incubators), and connected per local codes.

•Fyrite or similar chemical-based CO2 analyzer.

BE ADVISED: INSTALLATION AND PRE-OPERATION PROCEDURE WILL TAKE AT LEAST 24 HOURS TO COMPLETE. DO NOT ATTEMPT TO RUSH THE PROCESS WITH SHORT CUTS TO THE PROCEDURES DESCRIBED IN THIS MANUAL.

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CERTIFICATION OF DECONTAMINATION:

We cannot accept for service or credit a product that has been exposed to or contaminated with chemically or biologically toxic or infectious substances or subjected to radioactivity without first being certified as free from said contamination.

Please have your Medical and/or Safety Officer sign this form certifying that proper decontamination procedures have been followed to render the product safe and free from hazards.

Any product forwarded to us, not accompanied by this form and a proper Return Goods Authorization Number, will be returned to the sender. To obtain a Return Goods Authorization Number, contact the Customer Relations Department at [phone].

We hereby certify that the LAB-LINE INSTRUMENTS, INC. product:

Model No.

and Serial No.

,

which is being forwarded has been properly decontaminated and is free from all toxic hazards, infectious agents, radioactivity and/or other hazards.

Company/Institution Name:
Street Address:
City:		State			Zip
Name (please print):		Title
Signature:
Phone:
DECONTAMINATION PROCEDURE (Be Specific):

Nature of Hazard That Required Decontamination:

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6

SECTION 1

INTRODUCTION

THANK YOU

for selecting Lab-Line Instruments for your equipment needs. For maximum value and ease of start-up,

PLEASE PROCEED AS FOLLOWS:

•Inspect the carton and contents for shipping damage. Notify the carrier immediately if damage is found.

•Use the Accessory Checklist when unpacking to verify that the complete unit has been received. Do not discard packing materials until all is accounted for.

•Read this Operation Manual thoroughly before deciding upon an appropriate location for the unit: you will want to consider the availability of power and other unit requirements, as well as user convenience.

•Insist that every operator of this unit becomes familiar with the Operation Section of this manual.

•Be sure to fill out the Warranty Registration Card and mail it in to Lab-Line Instruments within seven (7) days after receiving the unit.

IF

after reading this manual you should have any difficulties with the installation or operation instructions, please call:

Lab-Line Customer Relations Department

[phone] or [phone]

ALL RIGHT RESERVED

The information contained in this manual is the exclusive property of Lab-Line Instruments, Inc., and has been provided solely to enable the users of the equipment described herein to operate and maintain such equipment. Any other use of this information, or the reproduction or transmission of all or any portion of this manual without prior written consent of the manufacturer is expressly prohibited. © 2001, Lab-Line Instruments, Inc.

SECTION 2

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DESCRIPTION

314/316 SERIES AUTOMATIC CO2 INCUBATORS:

BASIC COMPONENTS, AN INITIAL OVERVIEW:

1		13
		14
2
3
4
5
6
7
8
15	9 10	11 12

1.	Control panel	9.	Inner glass door, handle shaft
2.	CO2 sensor	10.	Inner glass door
3.	CO2 inlet port	11.	Inner glass door, handle
4. Temperature sensor		12.	Outer door gasket
5.	Chamber gasket	13.	CO2 sample port
6.	Chamber shelves	14.	Fan Cover
7.	Sample port inlet	15.	Humidity Trough
8.	Leveling feet

DESCRIPTION: (Con’t)

8

314/316 SERIES AUTOMATIC CO2 INCUBATORS: (Con’t)

BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)

CHAMBER CEILING, INTERIOR

	CIRCULATING FAN
	REAR PANEL VIEW, EXTERIOR:
1	4
2	5
3	6

1.GAS INLET PORT

2.CORDSET

3.CIRCUIT BREAKERS

4.RS232 DATA PINS CONNECTOR

5.POWER SWITCH

6.OPTIONAL ALARM RELAY OUTPUT

DESCRIPTION: (Con’t)

314/316 SERIES AUTOMATIC CO2 INCUBATORS:

9

CO2 levels are automatically set at 5%. These units control CO2 concentrations, as well as temperature, to create a growth environment ideal for tissue cultures, plants, cells, viruses and related organisms.

Incoming 100%, CO2, from an external source provided by the user, passes into the gas inlet port located on the rear panel of the unit. Heated air is moved uniformly throughout the chamber by a circulating fan located in the top of the chamber. A CO2 access port on the control panel permits sampling of the chamber atmosphere.

These incubators provide precise and reliable control of carbon dioxide through the use of a TC, thermal conductivity, sensor coupled with an easy-to- use command control console. All required operating commands are immediately accessible in a functional format on the front of the unit and permit rapid start-up, close monitoring and efficient control of incubation protocols.

Temperature is measured by a platinum RTD, resistance temperature detector, producing excellent control within the range, ±0.1ºC. This is combined with the exclusive SmartGas® algorithm method for controlling CO2 within the range ±0.1%. The result is a chamber that produces excellent environmental conditions for the most sensitive of incubations.

Audible and visual alarms provide a signal to the user of any deviation in the chamber temperature of ±1.0ºC, or carbon dioxide of ±1.0% CO2. In addition, other signals alert the user to power failures and door openings.

An independent, adjustable hydraulic high limit thermostat will limit the temperature in the event of a problem with the main temperature controller.

To conserve gas, the unit’s CO2 solenoid valve, as well as the circulating fan motor, shuts off automatically any time the door is opened.

A double-door system has a tempered glass inner door that provides unobstructed viewing of the entire chamber.

314 series chambers are constructed of seamless stainless steel for cleaning ease and maintenance; 2 stainless steel shelves are standard—a total capacity is 5 shelves. 316 series incubators feature a fungus and bacteria resistant copper chamber.

Both models have a stainless steel outer cabinet with a powder coated finish for easy cleaning.

DOUBLE-DOOR CONSTRUCTION WITH INNER DOOR DEFOGGER: 314/316 MODELS:

A tempered glass inner door allows observation of a chamber without disturbing the chamber environment. Inside the steel outer door are heaters that can be turned on to defog the glass door when necessary. Each door seals to a one-piece gasket for a leak-tight environment.

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DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS:

BASIC COMPONENTS, AN INITIAL OVERVIEW:

1

2 3 4 5 6 7 8 9 10

1.	AIR & CO2	5.	TEMPERATURE SENSOR
	FLOWMETERS	6.	CHAMBER GASKET
2.	UNIT FEET	7.	INNER GLASS DOOR, HANDLE SHAFT
3.	CO2 ACCESS PORT	8.	INNER GLASS DOOR
4.	CHAMBER SHELVES	9.	INNER GLASS DOOR, HANDLE
		10.	OUTER STEEL DOOR/GASKET

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DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS: (Con’t)

BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)

CHAMBER CEILING, INTERIOR

CIRCULATING FAN

REAR PANEL VIEW, EXTERIOR:

CO2

1	2

1.CO2 INLET PORT

2.CORDSET

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DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS:

CO2 levels are manually adjusted utilizing flowmeters. Flo-Thru control of CO2 concentrations and temperature create a growth environment ideal for tissue cultures, plants, cells, viruses and related organisms.

An incoming gas mixture passes through the plenum and is gently circulated throughout the chamber. Separate flowmeters adjust air and CO2 rates and an access port on the control panel permits sampling of the chamber atmosphere.

2 hydraulic thermostats: one is an adjustable setpoint control unit, the other is a non-adjustable, over-temperature-limiting unit. It is factory pre-set to regulate temperature in order to protect the chamber.

Heaters on the outside of the chamber inner walls are positioned for uniform heat distribution, while high-density fiberglass insulation minimizes heat loss.

A double-door system has a tempered glass inner door that provides unobstructed viewing of the entire chamber.

Model 315 and 315-1 interiors are constructed of seamless stainless steel for cleaning ease and maintenance. 2 stainless steel shelves are standard—a total capacity is 5 shelves.

Models 317 and 317-1 have a seamless copper interior that acts to reduce the growth of fungus and bacteria thereby minimizing contamination of

cultures. 2 copper shelves are standard with room for 3 additional shelves.

All models have a steel outer cabinet with a powder coated finish for easy cleaning.

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		SECTION 3
		SPECIFICATIONS
314/316 SERIES AUTOMATIC CO2 INCUBATORS:
	ELECTRICAL REQUIREMENTS:	120 V, 60 Hz, 200 Watts, 1.7 Amps,
314, 316:
314-1, 316-1:		230/240 V, 50 Hz, 200 Watts, 0.8 Amps,
	TEMPERATURE:	Ambient +5ºC to 60ºC
	RANGE:
	CONTROL:	±0.1ºC at 37ºC.
	UNIFORMITY IN CHAMBER:	Within the chamber, ±0.25ºC
	ALGORITHM:	TEMPERATURE: PID
		CO2: SmartGas®
	CARBON DIOXIDE TENSION:	Range: 0% to 20%
		Control: ±0.1%
	VOLUME:	1.2 cubic feet (34 liters)
	EXTERIOR:	22½" W x 18" D x 19" H
		(57 x 46 x 48 cm)
	INTERIOR:	14" W x 12" D x 12" H
		(36 x 31 x 31 cm)
	SHELVES:	2 standard; space for 3 additional shelves.
	DIMENSIONS:	13-3/8” (tapering back to) 12-1/8" W x 11½" D
		(34~30.7 x 29.2 cm)
	SHIPPING WEIGHT:	70 lbs. (32 kg)

SPECIFICATIONS: (Con’t)

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315/317 SERIES FLO-THRU CO2 INCUBATORS:

ELECTRICAL REQUIREMENTS:	120 V, 60 Hz, 200 Watts, 1.7 Amps,
315, 317:
315-1, 317-1:	230/240 V, 50 Hz, 200 Watts, 0.8 Amps,
TEMPERATURE:	Slightly above ambient to 60ºC.
RANGE:
CONTROL:	±0.5ºC.
UNIFORMITY:	±1.0ºC
CARBON DIOXIDE RANGE:	From 0 to 20% of the mixture.
VOLUME:	1.2 cubic feet (34 liters)
DIMENSIONS:	23½" W x 17" D x 15" H
EXTERIOR:
INTERIOR:	(60 x 43 x 38 cm)
	14" W x 12" D x 12" H
	(36 x 31 x 31 cm)
SHELVES:	2 standard; space for 3 additional shelves.
DIMENSIONS:	11"W x 13½"D x 7"H (28 x 34 x 18 cm)
SHIPPING WEIGHT:	70 lbs. (32 kg)

UNIT’S ENVIRONMENTAL OPERATING CONDITIONS:

POLLUTION DEGREE: 2 INSTALLATION CATEGORY: II

ALTITUDE: 2000 Meters MSL (Mean Sea Level)

HUMIDITY: 80% maximum, non-condensing

ELECTRICAL SUPPLY: 120VAC or 240VAC VOLTAGE TOLERANCE: ±10% of normal rated line

TEMPERATURE: 15ºC to 40ºC

PRODUCT USAGE: This product is intended for use indoors only

SECTION 4 15