Multi-Parameter
Water Quality
TROLL
Instruction Sheet
Catalog No. 48670, 95150
CALIBRATION KIT
FOR THE POLAROGRAPHIC
D.O. SENSOR
APPLICATION
The D.O. Calibration Kit contains the following for performing an oxygen
saturation point calibration on the polarographic Dissolved Oxygen sensor:
• Battery-powered air pump
• Bubbler cup with aquarium stone, tubing, check valve and pinch valve
• Grey PVC adapter for holding the MP TROLL in top of bubbler cup
• 2 alkaline D-cells
®
TIP: Detailed calibration
instructions for the polarographic D.O. sensor may be
found in section 13 of the MultiParameter TROLL Operator's
Manual, on the Win-Situ
installation CD-ROM or
available in the Downloads
section of the In-Situ website.
TIP: If the polarographic D.O.
sensor is installed when you
receive the instrument, it will
be conditioned and ready to
calibrate.
TIP: Testing has shown that 10
hours of conditioning yields
very stable long-term
performance.
OXYGEN SATURATION CALIBRATION PROCEDURE
1 Install the polarographic Dissolved Oxygen sensor in the MP TROLL (see
instructions packaged with the sensor, or the MP TROLL operator's
manual).
2 Condition the newly-installed sensor as follows:
2A Connect the MP TROLL to a PC and establish a connection in Win-
Situ or Pocket-Situ.
2B Select the MP TROLL in the Navigation tree. All installed sensors will
be displayed. Powering of the D.O. sensor begins as soon as the
software recognizes the D.O. sensor and displays it in the Navigation
tree. This starts the conditioning process.
2C We recommend that you allow the sensor to condition for 2-10 hours
before calibrating.
TIP: It is not necessary to maintain the computer connection;
conditioning continues as long as the sensor is installed.
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Multi-Parameter Water Quality
TROLL
®
3 Meanwhile, prepare the bubbler:
3A Install the batteries in the portable air
pump as shown in the diagram on the
inside of the lid.
3B Fill the bubbler container almost to
Battery Orientation
the top with clean water. The
optimum amount depends on the volume of the
sensors installed in the MP TROLL.
TIP: Tap water is fine, unless it is high in salinity. It is
easier to achieve 100% oxygen saturation in low-salinity
water. Distilled water is readily available and works well.
3C About 10 minutes before calibration, turn on the
bubbler. Regulate bubbling with the pinch valve. For
best results, run at the full flow rate to achieve
saturation; after 10 minutes you can use the control
valve to match the water turbulence conditions
expected in the field.
4 When ready to calibrate, place the
adapter securely in the top of the
bubbler housing and insert the front
(sensor) end of the MP TROLL into
the adapter.
• Insure the D.O. sensor is not in
the aeration path.
• Insure the temperature sensor
is submerged at least 0.5” (12
mm) in the aerated water.
5 If not already connected, connect the MP TROLL to a PC,
launch the software and "find" the device.
BUBBLER CAL KIT
Catalog No. 48670, 95150
9 Before the DO Calibration Wizard starts, you will be asked
how you want to handle barometric pressure. Cable venting
is essential to obtain accurate measurement, and the
software cannot tell if this cable is vented, so please take a
moment to supply this information. Do one of the following:
• If the TROLL is on vented cable now and will be
deployed on vented cable, click No — and you may
want to check the “Don’t ask me this again” box.
• If the device is on vented cable now but will be
deployed on non-vented cable, click Yes. In the
next screen, check the box indicating non-vented
cable for deployment but vented cable for
calibration/programming.
• If the device is on non-vented cable now and will be
deployed on non-vented cable, click Yes. In the
next screen, check the box indicating non-vented
cable for deployment and enter a barometric
pressure value. For help in supplying this
information, see Section 9 of the MP TROLL
Operator's manual (Monitoring Barometric
Pressure).
6 Select the MP TROLL in the Navigation tree.
7 Select Dissolved Oxygen in the Parameters list.
Information on the sensor is shown, including its serial
number (SN) and recent calibration information, as shown
in the screen below.
8 Select Calibrate.
7
8
9
After dealing with barometric pressure a new screen will
be shown (top of page 3).
10 First, select the number of calibration points.
1 point—100% D.O. (with the bubbler)
2 points—100% and 0% D.O.
Normally you will select 1 point.
TIP: 0% D.O. is calibrated in an oxygen-depleted
solution such as sodium sulfite or nitrogen-saturated
water. A 0% calibration is recommended:
• when you expect to measure very low D.O. values
(< 5 mg/L),
• when required by Standard Operating Procedures.
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Multi-Parameter Water Quality
TROLL
®
Status indicators & controls during stabilization:
NOT TESTED may be displayed before the calibration
10
11
begins.
12
13
14
11 Select the membrane type—stamped on the membrane
module. (When calibrating in water, the thickness is not
crucial.)
12 Select Water as the medium for the first calibration point.
13 Select the stimulus at saturation:
• Default—This value is calculated by the software at the
current temperature and barometric pressure.
• User Set—Enter a value from a look-up table if required.
14 Click Next to continue. A screen like this will be displayed:
UNSTABLE indicates the sensor response does not
meet the criteria for a valid calibration point. All
parameters start out at “Unstable” status.
NOMINAL indicates the change in the sensor response
over time meets a relaxed stability criterion. “Nominal”
stability will occur first, and can be accepted to shorten
the calibration time.
STABLE indicates the change in the sensor response
over time meets the stability criteria for a valid
calibration point. Calibration advances automatically.
Sensor Reading: Current sensor response (nanoAmps).
Sensor Deviation: Change in response between the last
two readings. This enables you to follow the progress
of the stabilization, but deviation from the previous
reading is not necessarily the best indicator of stability
as the software is looking at longer-term trends.
Run button: Starts the calibration.
Accept button: Becomes available when Nominal
stability is reached You may accept the early value, or
wait for complete stability. If you accept the early value,
the status will be designated “USER SET” in the
calibration report. (For more on calibration reports and
the difference between Nominal and Stable, see
Section 10 of the MP TROLL Operator's Manual.)
15
15 With the sensor situated in the bubbled water, click or tap
Run to start the Oxygen Saturation calibration point.
The display will continuously update as readings are taken
and compared against the stabilization criteria.
Stop button: Stops the current phase of the calibration.
Back button: Goes to the previous screen (when
available).
Next button: Proceeds to the next screen (when
available).
Cancel button: Cancels the calibration.
TIP: The current temperature and barometric pressure are
used to calculate the stimulus. If the calibration stimulus
was entered from a look-up table at step 13 and the
temperature is not as expected, you may select Stop, then
Back to retrieve the Setup page and re-enter the User Set
stimulus.
16 When readings have stabilized (or you click to Accept the
Nominal result), the calibration will advance automatically.
For a one-point calibration, the final screen appears. (In
the screen shown below, a one-point calibration at
saturation was performed.)
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Multi-Parameter Water Quality
17 To finalize the calibration click Finish.
TROLL
®
BUBBLER CAL KIT
Catalog No. 48670, 95150
SENSOR SLOPE AND OFFSET
The slope of a properly functioning sensor as calculated
during the calibration process will typically be in the following
ranges, depending on the membrane thickness:
1-mil membrane: between 30 and 67 nA/(mg/L)
2-mil membrane: between 15 and 34 nA/(mg/L)
If the calculated slope is much outside the stated range, the
sensor may need maintenance. Refer to “Sensor Care and
Handling” in Section 13 of the MP TROLL Operator's Manual.
The default offset for Quick Cal and 1-point calibrations is 2
nA. Offsets for 2-point calibrations should fall under 10 nA.
17
The values will be written to the sensor and you will be
asked if you want to see the calibration report.
TIP: You can look at the calibration report right after
calibrating, or at any time. See “Calibration History” in
Section 10 of the MP TROLL Operator's Manual for more
information on calibration reports.
The polarographic D.O. sensor is now calibrated and ready to
take readings. To confirm this, take a reading by selecting the
D.O. sensor in the Navigation tree and clicking the Read
button.
TIP: If the units for the reading are not what you expect, it
is easy to change the units selection: In Win-Situ, select
Preferences on the Options menu. In Pocket-Situ, select
the Home site, then tap Setup in the command bar.
REPLACEMENT PARTS CATALOG NO.
Bubbler housing ......................................................... 48080
Bubbler base .............................................................. 48090
Portable air pump ...................................................... 48730
PVC adapter for MP TROLL 9000 ............................. 48660
PVC adapter for MP TROLL 9500 ............................. 53230
3.028" o-ring for base ................................................ 48260
Check valve ............................................................... 48540
Clamp ........................................................................ 48570
Elbow fittings (3) ........................................................ 48740
Aquarium stone (3) .................................................... 48750
Complete kit for MP TROLL 9000 ............................. 48670
Complete kit for MP TROLL 9500 .............................. 95150
1 800 4INSITU
(toll-free, US and Canada) or 970 498 1500 www.in-situ.com
Due to continuing product development this information is subject to change without notice. In-Situ and the In-Situ logo, Win-Situ, Pocket-Situ,
TROLL, and RDO are trademarks or registered trademarks of In-Situ Inc. Copyright © 2005 by In-Situ Inc. All rights reserved.
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0048672 rev. 002 09/05
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