Instruction Manual
HI 84432
TITRATABLE ACIDITY
MINITITRATOR & pH METER
for Fruit Juices
www.hannainst.com
1
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter.
If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
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TABLE OF CONTENTS
PRELIMINARY EXAMINATION................................................................................................. 4
GENERAL DESCRIPTION ........................................................................................................ 4
SPECIFICATIONS ................................................................................................................... 6
PRINCIPLE OF OPERATION .................................................................................................... 7
FUNCTIONAL AND PHYSICAL DESCRIPTION............................................................................. 8
TITRATOR STARTUP ........................................................................................................... 11
SETUP CONFIGURATION MENU .......................................................................................... 12
ELECTRODE PREPARATION .................................................................................................. 16
ELECTRODE CALIBRATION PROCEDURE................................................................................. 17
pH BUFFER TEMPERATURE DEPENDENCE............................................................................ 22
PUMP TUBE INSTALLATION................................................................................................. 23
PURGE .............................................................................................................................. 24
PUMP CALIBRATION PROCEDURE ........................................................................................ 26
TITRATION PROCEDURE ..................................................................................................... 29
pH MEASUREMENT ............................................................................................................ 34
TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only) ............................... 38
TROUBLESHOOTING GUIDE ................................................................................................ 40
ELECTRODE CONDITIONING AND MAINTENANCE .................................................................. 41
ACCESSORIES .....................................................................................................................42
WARRANTY........................................................................................................................43
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
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PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 84432 minititrator is supplied complete with:
• HI 1131B pH electrode
• HI 7662-M Temperature probe
• HI 84432-50 Titrant solution (100 mL)
• HI 84432-55 Pump calibration solution (100 mL)
• HI 70004 pH 4.01 buffer solution (2 x 20 mL)
• HI 70007 pH 7.01 buffer solution (2 x 20 mL)
• HI 700082 pH 8.20 buffer solution (2 x 20 mL)
• Two 100 mL beakers
• Tube set with dispensing tip
• Stir bars (medium 2 pcs.)
• 12 Vdc power adapter
• Instruction manual
Note: Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
GENERAL DESCRIPTION
The HI 84432 is an easy to use microprocessor-based automatic minititrator and pH meter designed
for the rapid and accurate analysis of Total Titratable Acidity in fruit juices. By eliminating subjective
factors including color indicators, errors in mathematical calculations or erratic titrant additions from
the measurement, the HI 84432 makes Total Titratable acidity analysis precisely. This will quickly
become a valuable acidity analysis tool of fruit juices.
The instrument benefits from Hanna’s many years of experience as a manufacturer of quality
analytical instruments. A clear and well-designed user interface makes the instrument intuitive and
simple to use. A dedicated HELP key aids in set-up, calibration, status and troubleshooting.
By simply pressing the START key, the HI 84432 automatically starts pump operation and titrates
the sample to the end point. The HI 84432 has a simple and accurate peristaltic pump to ensure the
best accuracy and repeatability. By performing pump calibration with the Hanna standard provided,
the instrument accuracy is ensured.
The instrument employs a powerful and effective built-in algorithm to analyze the pH response to
determine the exact pH endpoint, then uses this to make the necessary calculations.
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The Titratable Acidity determination is instantaneously displayed in selected measurement units
on the large dot matrix display. The instrument is ready for the next analysis immediately.
Other features:
• Log on demand up to 100 samples (50 for pH measurement; 50 for titration results)
• GLP feature, to view last calibration data for pH electrode and pump
MEASUREMENT SIGNIFICANCE
The HI 84432 measures the concentration of titratable hydrogen ions contained in the fruit juice
samples, by neutralization with a strong base solution to a fixed pH. This value includes all the
substances of an acidic nature in the fruit juice: free hydrogen ions, organic acids, acid salts and
cathions. Because the organic acid is the most acidic component of fruit juices that react with strong
bases solutions, the titratable acidity is usually expressed as g/L or g/100 mL of the predominant
acid contained:
• Citric acid is present in many fruit species.
• Tartaric acid is essentially found in grapes.
• Malic acid is present in many fruit species, sometimes together with citric acid or tartaric
acid in unripe grapes.
The titratable acidity of fruit is an important parameter in determining fruit maturity.
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The HI 84432 minititrator uses a method based on the Official Methods of Analysis of AOAC International.
The fruit juice is titrated with a sodium hydroxide solution until the end point at 8.2 pH is reached
(determined by potentiometric method).
Additionally the HI 84432 has a built-in pH meter for pH measurement (electrode and meter must
be calibrated).
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SPECIFICATIONS
Titrator Range Titratable acidity (low range): 15 mL sample
g/100 mL
g/100 mL
g/100 mL
Titratable acidity (high range): 2 mL sample
g/100 mL
g/100 mL
g/100 mL
Resolution 0.01%
Accuracy 5% of reading or ± 0.02
Titration method Acid-base titration
Principle End point titration: 8.20 pH
Pump debit 0.5 mL/min
Stirring speed 600 rpm
Log data Up to 50 samples
pH meter pH meter -2.0 to 16.0 pH / -2.00 to 16.00 pH
pH Resolution 0.1 pH / 0.01 pH
pH Accuracy ± 0.01 pH
pH Calibration 1, 2 or 3 calibration points;
3 available buffers (4.01; 7.01; 8.20)
Temperature manual or automatic from
compensation -20 to 120 °C (-4 to 248 °F)
Log data Up to 50 samples
Temperature Range -20.0 to 120.0 °C (-4.0 to 248.0 °F)
Resolution 0.1 °C
Accuracy ±0.4 °C without probe error
Electrode HI 1131B (included)
Temperature Probe HI 7662-M (included)
Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing
Power supply 12 Vdc power adapter
Dimensions 208 × 214 × 163 mm (8.2×8.4×6.4”) (with beaker)
Weight 2200 g (77 oz.)
as citric acid: 0.20 - 1.20% CA
as tartaric acid: 0.23 - 1.41% TA
as malic acid: 0.21 - 1.26% MA
as citric acid: 0.80 - 8.00% CA
as tartaric acid: 0.94 - 9.30% TA
as malic acid: 0.84 - 8.30% MA
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REQUIRED REAGENTS
Code Description Quantity/Test
HI 84432 - 50 Titrant 1 mL
HI 84432 - 55 Pump Calibration Solution 2 mL
PRINCIPLE OF OPERATION
Fruit juice acidity is determined by neutralization of all available hydrogen ions present in the
sample, with a strong base solution:
+
H
+HO- H2O
In an ideal solution, the end point of an acid titration corresponds stoichiometrically to the complete
neutralization of the acids present.
The HI 84432 minititrator is designed to determine the acidity of fruit juices by potentiometric
titration. The result of the titration are displayed in % (g/100 mL) of the predominant acid (citric,
malic or tartaric acid).
Using a pH electrode allows an end point at 8.2 pH to be automatically detected. End point
detection by potentiometric method is more objective than the end point detection using the
indicators.
For precise results the sample and titrant volume and the titrant concentration must be known.
It is important to titrate fresh samples of the fruit juice. For increased measurement precision keep
the fruit juices in capped vessels (avoid prolonged exposure to air). Also avoid refrigerating the juice
as significant portion of the tartaric acid (ex. grapes juice) will precipitate out and the results
obtained will be lower then expected results.
Titratable Acidity in fruit juices, as performed on the HI 84432 minititrator, utilizes a simple sample
preparation, a high quality peristaltic dosing pump for titrant, potentiometric endpoint detector and
instantaneous computations. To maintain the high precision of the titrator, a simple pump calibration
procedure is required. The pump calibration involves the analysis of a known volume of a known
solution (standard provided) and compensates for changes in pump dosing that may occur due to
many factors including tube stretching or aging. This procedure should be performed a minimum of
daily.
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FUNCTIONAL AND PHYSICAL DESCRIPTION
OVERHEAD VIEW
1. Titrant bottle
2. Graphic LCD
3. Keypad
4. Dedicated guide tube
for Temperature Probe
5. Electrode holder
6. Dedicated guide tube for titrant tip
7. Peristaltic pump tube
FRONT VIEW
REAR VIEW
8. Temperature probe
9. pH Electrode
10. Beaker
11. BNC electrode connector
12. Temperature probe socket
13. Power switch
14. Power adapter connector
15. Peristaltic pump
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KEYPAD FUNCTION AND INDICATORS
ESC - used to leave the current screen and to return either to the previous screen or to the
main screen. When pressed while modifying a parameter within the SETUP menu,
exits parameter without changing it.
/
- used to modify the parameters’ values, to scroll the information displayed while
viewing a help or to move between the options from the instrument’s SETUP
HELP - used to access/leave the instrument’s contextual help
MENU - used to enter SETUP, Recall or GLP selection menu, while instrument is in pH or
Titration main screen
STIR - used to start/stop the stirrer.
Note: The stirrer starts automatically during pump calibration and titration and cannot be
stopped by pressing STIR key.
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GUIDE TO INDICATORS
During the instrument’s operation information is displayed on the LCD.
Displayed icons:
Unstable reading.
Stirrer on.
1. Current time and instrument mode information (pH meter or Titrator)
2. Main reading information
3. Instrument status information
4. Functional key area
5. Indicates that the displayed value can be changed using ARROW keys
6. Temperature value (Manual or Automatic)
7. pH temperature compensation mode (Manual or Automatic)
8. Stirrer and reading status area
PERISTALTIC PUMP
Peristaltic pumps are self priming. Liquid never contacts the pump components. The titrant tubing
is pressed along the rotating rollers of the pump. The rollers compress the tubing, driving the
titrant to the dispensing tip.
Note: It may be necessary to compress the tubing against rollers with your finger to hasten the
filling of the tubing the first time.
Pump running.
Parameter can be changed.
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TITRATOR STARTUP
This is a general outline of the steps required to make a titration. The following topics are
expanded upon each section that follows.
• Place the instrument on a flat table. Do not place the instrument in direct sun light.
• Connect the power adapter to the instrument.
• Turn the instrument ON using the power switch from the rear panel of the instrument.
• Set up the instrument. See the “Setup Configuration Menu” section for details. Pay attention
to the Acidity Unit.
• Connect the pH sensor and temperature probe to the instrument.
• Calibrate the pH electrode. At least a single point calibration is necessary for titration.
• Place the peristaltic pump tube on the pump (inlet tube is connected with the reagent bottle,
outlet tube is connected with the dosing tip). See the “Pump Tube Replacement” section for
the procedure.
• Remove the reagent bottle cap and replace with the bottle cap with tubes. Place the reagent
bottle in the appropriate place on the titrator top.
• Purge the titrant. Verify titrant is leaving titrant tip.
• Calibrate the pump.
• Prepare the sample.
Note: Different volumes of juice are required for measurement accuracy. See SAMPLE PREPARATION
for details.
• Run a titration and log sample results.
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SETUP CONFIGURATION MENU
The titrator’s setup configuration menu may be accessed from the pH or titration screens by
pressing the MENU key, then Setup.
A list of setup parameters will be displayed with currently configured setting.
While in the setup menu it is possible to modify the instrument’s operation parameters. The
ARROW keys permit the user to scroll the setup parameters.
Press HELP to view the contextual help.
Press ESC to return to the main screen.
Concentration unit
Options: %CA; %TA; %MA.
Press the corresponding function key to change
the option.
Calibration timeout
Options: Disabled or 1 to 7 days.
This option is used to set the number of days
before the pH calibration expired warning message
is flagged.
Press Modify to access the calibration timeout
value modify parameter.
pH resolution
Use the ARROW keys in order to increase/
decrease the value.
Press Accept to confirm or ESC to return to the
setup menu without saving the new value.
Options: 0.1, 0.01.
Press the displayed function key in order to change
the pH resolution.
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Temperature unit
Backlight
Options: °C, °F.
Press the function key in order to change the
temperature unit.
Options: 0 to 8.
Press Modify to access the backlight level.
Contrast
Use the ARROW keys or
increase/decrease the displayed constrast.
Press Accept to confirm or ESC to return to the
setup menu.
Option: 0 to 20.
This option is used to set the display’s contrast.
Press Modify to change the display’s contrast.
Use the ARROW keys or / in order to
increase/decrease the value.
Press Accept to confirm the value or ESC to
return to the setup menu.
/ in order to
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Date / Time
Time format
This option is used to set the instrument’s date
and time.
Press Modify to change the date/time.
Press / to highlight the value to be
modified (year, month, day, hour, minute or
second). Use the ARROW keys to change the
value.
Press Accept to confirm the new value or ESC to
return to the setup.
Option: AM/PM or 24 hours.
Press the functional key to select the new value.
Date format
Press Modify to change the Date Format.
Use the ARROW keys to select the desired
format.
Press Accept to confirm the value or ESC to
return to the setup menu.
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Language
Beep On
Meter information
Press the corresponding function key to change
the language.
If the new selected language cannot be loaded,
the previously selected language will be reloaded.
If no language can be loaded at startup the
instrument will work in the “safe mode”. In “safe
mode” all the messages are displayed in English
and help information are not available.
Press the function key to select the new option.
When enabled, a short beep is heard every time a
key is pressed or when the calibration can be
confirmed.
A long beep alert sounds when the pressed key is
not active or a wrong condition is detected while
in calibration.
Press Select to view the firmware version, language
version, mV factory calibration date and time and
temperature factory calibration date and time.
Press ESC to return to the Setup mode.
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ELECTRODE PREPARATION
PREPARATION PROCEDURE
Remove the electrode protective cap.
DO NOT BE ALARMED IF ANY SALT DEPOSITS ARE PRESENT. This is normal with electrodes and
they will disappear when rinsed with distilled water.
During transport, tiny bubbles of air may have formed inside the glass bulb. The electrode cannot
function properly under these conditions. These bubbles can be removed by "shaking down" the
electrode as you would do with a glass thermometer.
For better performance, soak the electrode in HI 70300 Storage Solution for at least one hour,
before using.
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ELECTRODE CALIBRATION PROCEDURE
It is recommended to calibrate the instrument frequently, especially if high accuracy is required.
The pH electrode should be recalibrated:
a) Whenever the pH electrode is replaced
b) At least once a week, but daily is advised
c) After electrode is cleaned
d) When high accuracy is required
e) If the pH calibration expired warning is displayed during measurement. Every time you clean
the electrode calibrate the instrument (see the “pH Cleaning Procedure” section) and use fresh buffers.
PROCEDURE
A single, two or three-points calibration can be performed, using the three predefined buffers
4.01, 7.01 and 8.20 pH. For a single point calibration any of the three buffers may be used,
but using 8.20 pH is recommended.
Note: The HI 84432 will not accept other pH buffers for calibration.
• Pour small quantities of selected buffer solutions into clean beakers. For accurate calibration
use two beakers for each buffer solution, the first one for rinsing the electrode and the second
one for calibration.
• Put a magnetic stir bar in each beaker with the calibration buffer solution.
• Remove the protective cap, open the fill hole and rinse the electrodes with some of the buffer
solution to be used for the first calibration point.
• Put the first beaker with calibration buffer in the beaker holder.
• Place the electrode holder on the top of the beaker and secure it by turning clockwise.
• Immerse the pH and the temperature probe approximately 2 cm (0.8”) into the buffer paying
attention not to touch the stir bar.
To select Electrode calibration screen follow the next steps:
• From pH meter screen press CAL function key then Electrode.
• From Titrator screen press CAL function key then Electrode.
• The electrode calibration screen will be displayed.
Point 1 calibration
• The 8.20 buffer will be selected by default. If necessary press the ARROW keys in order to
select a different buffer value.
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• The
becomes stable.
(unstable measurement) symbol will be shown on the display until the reading
• When the reading is stable and close to the selected buffer, the
symbol will disappear and the Confirm key will become active.
• Press Confirm to confirm the calibration.
• Press ESC to exit calibration.
Point 2 calibration
• The calibrated value will be shown on the display and the second expected buffer value will
be displayed.
• Remove the electrode holder with electrodes from the top of the beaker.
• Place the second buffer into beaker and place in beaker holder. Rinse the electrodes in a
beaker containing the second buffer rinsing solution.
• Place the electrode holder (with electrodes) on the top of the beaker, lock holder by turning.
• If necessary press the ARROW keys in order to select a different buffer value.
• The
(unstable measurement) symbol will be shown on the display until the reading
becomes stable.
(unstable measurement)
• When the reading is stable and close to the selected buffer, the
symbol will disappear and the Confirm key will become active.
• Press Confirm to confirm the calibration.
• The calibrated value will be shown on the display and the third expected buffer value will be
automatically selected.
• After the second calibration point has been confirmed, press ESC to exit without performing
the third calibration point.
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(unstable measurement)
Point 3 calibration
• Remove the electrode holder with electrode from the top of the beaker.
• Place the third buffer solution in a beaker and place in beaker holder. Rinse the probes in a
beaker with third buffer rinsing solution.
• Place the electrode holder (with electrode) in the beaker with third buffer and secure holder by
locking.
•
The (unstable measurement) symbol will be shown on the display until the reading
becomes stable.
• When the reading is stable and close to the selected buffer, the
symbol will disappear and the Confirm key will become active.
• Press Confirm to confirm the calibration. The instrument stores the calibration value and returns
to pH meter/titrator calibration menu, where the date and time for the last pH will be updated.
Notes:
• A buffer confirmed during the calibration process is removed from the list of
calibration buffers available for further calibration points.
• If the value measured by the instrument is not close to the selected buffer a “Wrong
Buffer” error message will be shown on the display.
Check if the correct buffer has been used or regenerate the pH electrodes by
following the Cleaning Procedure (see the “pH Cleaning Procedure” section). If
necessary change the buffer or the electrode.
• If the measured offset isn’t within the preset limits the meter will display the
message “Buffer Contaminated” alternatively with ”Electrode Dirty/Broken”.
(unstable measurement)
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• If the computed slope isn’t within the preset limits the meter will display the
message “Wrong Slope”. If the slope is too high the symbol
displayed. If the slope is too low the symbol
• If the “Wrong Old Slope” error message is displayed, an inconsistency exists
between the current and the previous (old) calibration. Clear the calibration parameters
by pressing Clear and proceed with calibration from the current calibration point. The
instrument will keep all the confirmed values during the current calibration point.
will be displayed.
will be
• If the temperature reading is out of the defined temperature range of the buffer (0 to
45°C) the “Wrong Buffer Temperature” error message will be displayed, and the
symbol °C will blink on the display. Calibration cannot be confirmed in this
situation.
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Notes: • To clear a previous calibration and to return to the default value, press Clear at any
time after entering calibration mode. The “Calibration cleared” message will be
shown for a few seconds on the display. If Clear is invoked during the first calibration
point the instrument returns to the measurement mode.
• The Clear key is displayed only if a previous calibration exists.
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pH BUFFER TEMPERATURE DEPENDENCE
The temperature has an effect on pH. The calibration buffer solutions are affected by temperature
changes to a lesser degree than normal solutions. During calibration the instrument will
automatically calibrate to the pH value corresponding to the measured or set temperature.
During calibration the instrument will display the pH buffer value at 25 °C.
PMETSREFFUBHp
C°F°10.410.702.8
023 10.431.783.8
514 00.401.743.8
0105 00.470.713.8
5195 00.440.772.8
0286 00.430.732.8
5277 10.410.702.8
0
368 20.400.771.8
5359 30.499.641.8
04401 40.489.611.8
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PUMP TUBE INSTALLATION
To mount the new peristaltic pump tube follow next steps:
• Position one tubing ferrule ring on its location.
• Stretch the tube over the peristaltic pump rollers.
• Fix the second tube ferrulering ring on its location.
• Attach the tube to the reagent bottle.
Note: Purge the peristaltic pump until drops of reagent
appears on the dosing tip by pressing the PURGE key from the titrator main screen. It may
be necessary to press the tubing against rollers with your finger to hasten the filling of the
tubing the first time.
To remove the tube of the peristaltic pump follow next steps:
Caution: Purge line with water to flush titrant solution from tube.
• Detach the tubes system from the reagent bottle.
• Grasp one ferrule ring of the peristaltic pump tube.
• Pull the tube until the ferrule rings are taken out from their location.
• Remove the other side of the tube.
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PURGE
Purging should be performed:
• if you notice there is no titrant in the tip;
• whenever the tube of the peristaltic pump is replaced;
• whenever the titrant is changed or a new bottle is used;
• before starting a pump calibration;
• before starting a lot of titrations.
In order to start purging, press the Purge key from the titrator main screen. The purging stops
automatically after 5 minutes.
To access the Purge key follow the next steps.
• From the instrument main screen (pH meter screen) press “Titrator” function key.
The instrument will display the next screen if any of the following conditions exist:
- the meter hasn’t been calibrated in 8.20 pH buffer
- the pH calibration has expired
- a pump calibration hasn’t been performed or more than 3 days have passed since the last
pump calibration.
• Press CAL to access the titration calibration menu where electrode and pump calibration may
be accessed.
• Press HELP to view the contextual help.
• Press Continue or ESC to skip the message and enter titrator main screen.
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• Press Purge to begin a purge cycle.
The purging stops automatically after 5 minutes.
To stop purging at any time and return to the main screen press ESC or Stop.
During a purge, a countdown timer displays remaining time in 5 minute purge.
Press Pause to interrupt the purge process.
Press Pause or Stop (by pressing the corresponding function key in the purge screen)
• after the first drops of fresh titrant appear at the dosing tip
• in case an error condition is observed (empty titrant bottle, tubes or dosing tip disconected,
pump error)
• if you want to resume at a later time
If Pause is pressed the next screen is displayed:
Press Resume to continue purging.
After the 5 minutes purging interval has elapsed the “Completed” message is displayed.
Another purge period can be initiated by pressing Restart or press ESC to return to main titrator
screen.
25
PUMP CALIBRATION PROCEDURE
Please set up the instrument before performing the pump calibration.
The calibration of the pump must be performed each time the pump tube, the reagent bottle or
the pH electrode is changed. It is recommended to daily perform the pump calibration before you
start titrations.
Verify: The electrode has been calibrated in 8.20 pH buffer.
• Sample preparation: Precisely measure 2 mL of HI 84432-55
Pump Calibration Solution and add it to a clean beaker.
Fill the beaker up to the 50 mL mark with the distilled or
deionized water.
• Place the stir bar into the beaker and then place the beaker in
the appropriate place on the instrument top.
• Place the electrode holder on the top of the beaker and secure
it by turning clockwise.
• Before immersing the pH electrode and temperature probe
carefully rinse with deionized water.
• Blot dry using soft tissue. Do not rub.
• Insert the calibrated pH electrode and the temperature probe
through the electrode holder and immerse pH electrode bulb
approximatively 2 cm (0.8”) into the standard paying attention
not to touch the stir bar.
• Insert the dosing tip in the appropriate holder place through
guide tube. Immerse the tip into solution.
Note: The chemical reagents may be hazardous if improperly
handled. Read Health and Safety Data Sheets before
performing the test.
• From the titrator main screen press CAL.
The instrument displays the date and time of the last electrode calibration, and the date and
time of the last pump calibration, or calibration expired messages.
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• Press Pump.
The next screen will be displayed.
• Press Start.
• After the pump calibration is started, on the upper right side of the display two animations
will be shown in order to indicate that the pump and the stirrer are working. On the lower
right side of the display is shown the amount of time that has passed since the beginning of
the calibration.
• After the pump calibration is complete a confirmation message is displayed for a few seconds,
then the instrument will return to the titrator calibration menu and will display the new time
and date for the last pump calibration.
27
Notes: • The calibration of the pump is dependent on concentration unit selected during SETUP.
• If an erroneous situation is encountered during the calibration, an error message is
displayed and the calibration can be restarted by pressing Restart.
• Use a new sample of standard and clean electrode and temperature probe before
pressing Restart.
• If the calibration doesn’t complete within 6 minutes the error message “Too much
standard” will be displayed and the calibration can be restarted by pressing Restart
after a new standard is prepared.
28
TITRATION PROCEDURE
Verify: The instrument has been calibrated (pH and pump) before performing a lot of titrations.
An electrode calibration in at least one point (8.20 pH buffer) is recommended.
In order to increase the result accuracy, the measuring range was divided in two ranges:
Low range (LR): 0.20 to 1.20% CA
High range (HR): 0.80 to 8.00% CA
Depending on the expected sample concentration, different sample volume must be used for each
range, as followings:
• Sample preparation: Accurately measure volume of juice required to a clean dry beaker.
For Low range 0.20 to 1.20% CA use 15.00 mL
For High range 0.80 to 8.00% CA use 2.00 mL
Fill the beaker to the 50 mL volume mark with deionized water.
Notes: • Check the pH of distilled water. If necessary,
adjust the pH to 8.2 by adding 1-2 drops of
titrant solution.
• Fruit juices samples must be stored in capped
bottles. Avoid disturbing any sediment in the
sample.
• Place the stir bar into the beaker and then place the
beaker in the appropriate place on the instrument top.
• Rinse off pH electrode and temperature probe with deionized
water into a waste container. Blot dry. Do not rub.
• Place the electrode holder with electrodes on the top of
the beaker and secure it by turning clockwise.
• Verify the pH and the temperature probes do not to touch
the stir bar. Use O-Rings provided to secure the pH
electrode in holder if necessary.
• Verify the dosing tip in the appropriate holder place and
immersed into the sample.
• From the titrator main screen press Titration. To enter
titrator main screen from pH meter mode press Titrator
and then Titration.
• Press Range to switch between low range (LR) and high range (HR).
• Verify the correct sample size is configured on instrument by seeing volume set displayed in
upper right corner.
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• Press Start to begin the titration process.
• After the titration is started two animations will be shown on the upper right side of the
display to indicate that the pump and the stirrer are running. On the lower right side of the
display is shown the period of time since the titration has been started.
• After the titration is complete, the concentration value is displayed in the selected unit.
• Press Unit to change the display unit.
• Press LOG to record the concentration value into the instrument’s memory.
A message will be displayed for a few seconds indicating the amount of the free log space.
50 log samples can be recorded in the instrument’s memory. When the titrator free log space
is under 12% the message will be shown permanently.
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• If the concentration is out of limits an exceeded range limit message will be displayed
blinking and the message “Out of range” will be shown. Another titration can be initiated
by pressing Restart. Prepare a fresh sample and then press Restart.
Note: If the end-point is not reached or it is not recognized or the input reading is out of range,
an error message will be displayed. Also the sign “<” underrange or “>” overrange is
shown. The titration can be restarted after a new sample is prepared by pressing Restart.
TIPS FOR AN ACCURATE MEASUREMENT
• Calibrate the instrument in 8.20 pH buffer solution at least once a day, before you start to
perform measurements.
• Purge the peristaltic pump to have the fresh titrant when starting a new calibration.
• Calibrate the peristaltic pump daily before performing a set of analyses.
• Clean the electrode in order to remove possible coatings from bulb.
• Precision of the measurement can be improved by using volumetric pipettes for standard and
sample additions.
• Pump calibration can be checked by running 2 mL pump standard as an unknown. It should
read 3.2% CA on HR, and 0.42% CA on LR setting.
VIEW/DELETE TITRATOR RECORDED LOG DATA
Press MENU key while in Titrator main screen.
Press Recall to access the titrator recorded data.
The instrument will display a list of all the titration records stored in the titration log.
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Use the ARROW keys to scroll the stored records list.
If the saved concentration was out of range the “!” and “<” / “>” symbol (underrange/overrange)
is displayed in front of the reading.
Press Delete to enter record deleting mode.
Press Delete All to enter all records deleting mode.
Press More to view more information.
Press Unit to convert the result to other unit.
Use the ARROW keys when
Press ESC to return to the previous screen.
If Delete was pressed the instrument will ask for confirmation.
is displayed to scroll between the log records.
Use the ARROW keys to focus on the record to be deleted.
Press Confirm to delete the record or ESC to return to the previous screen.
Deleting a record will renumber the list of records.
If Delete All was pressed the instrument will ask for confirmation.
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Press Confirm to delete all the records or ESC to return to the previous screen.
If the titrator log is empty the message “No Records!” will be displayed.
TITRATOR GLP INFORMATION
Press MENU while in Titration mode and then GLP.
From this screen it is possible to select between viewing the electrode GLP or the pump GLP.
Press GLP elec. to view the electrode’s last calibration parameters and date.
Press GLP pump to view the pump’s last calibration time and date.
If GLP elec. is pressed one of the next screens will be displayed.
GLP contains a set of information regarding electrode calibration. The following items are
included in electrode GLP: the time and date of the last calibration, offset, slope, calibration
timeout and the calibration buffers. The buffers displayed in video inverse mode are from the
previous calibration.
If a calibration hasn’t been performed the message “Not Calibrated” will be displayed.
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If GLP pump is pressed, one of the next screens is displayed.
The pump GLP displays the Time and Date of the last pump calibration.
If a calibration hasn’t been performed the message “Not Calibrated” will be displayed.
pH MEASUREMENT
The HI 84432 may be used as a pH meter for direct measurements.
Verify that the instrument has been calibrated before taking pH measurements. Set the
instrument to pH meter. At power up the instrument enters pH meter mode. From titrator mode
press ESC until pH units are displayed.
If an electrode calibration hasn’t been performed, or the number of days exceedes calibration
time out value set, the message “CAL DUE” will blink on the left side of the display (see
Calibration timeout option in Setup for details).
Place calibrated pH electrode into electrode holder. Open the fill hole.
Rinse the pH tip with distilled or deionized water. Immerse the pH bulb (bottom 2 cm / 0.8”)
in the sample and stir gently for a few seconds.
For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip
with a few drops of the solution to be tested, before taking measurements. Press LOG to record
sample pH in instrument pH log.
If CAL DUE is displayed perform an electrode calibration.
Press MENU to access the instrument’s menu.
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Press HELP to view the contextual help, every time you need supplementary information. The
help is customized for every situation that can appear during instrument usage.
Press STIR to start/stop the stirrer.
Press Titrator to enter titrator mode.
Press CAL to access the calibration menu.
Press LOG to memorize the current reading. A message indicating the free log space will be
displayed for a few seconds.
In order to take pH measurements follow the next steps:
• Submerge the pH bulb 2 cm (0,8’’) and the temperature probe into the sample to be tested
and stir gently. Allow time for the electrode to stabilize. When the reading becomes stable
(unstable measurement) symbol will disappear.
the
• If the pH reading is less than -2.00 pH or greater than 16.00 pH the closest full-scale value
(-2.00 pH or 16.00 pH) will be displayed blinking.
If measurements are taken successively in different samples, it is recommended to rinse the
electrodes thoroughly with deionized water or tap water and then with some of the next sample
to prevent cross-contamination.
The pH measurement is affected by temperature. In order to have accurate pH measurements,
the temperature effect must be compensated for. To use the Automatic Temperature Compensation
(ATC) feature, connect and submerge the HI 7662-M temperature probe into the sample in the
dedicated guide tube and wait for a few seconds. The “ATC” message will be shown on the
display. Automatic Temperature Compensation will provide pH corrected values at the temperature
35
of measurements. If Manual Temperature Compensation (MTC) is desired, the temperature probe
must be disconnected from the instrument. The default temperature of 25 °C (77 °F) or the last
temperature reading will be displayed preceded by the symbol
The manually set temperature can now be adjusted with the ARROW keys (from -20.0 to 120.0 °C).
VIEW/DELETE RECORDED pH DATA
To view or delete previously logged pH records press MENU key while in pH meter screen.
Press Recall to access the pH recall. A list of records is stored in the pH log.
Use the ARROW keys to scroll the list of records.
Press More to see detailed information about the highlighted record.
Press Delete to enter record deleting mode. Press Delete All to enter all records deleting mode.
If More is pressed a complete set of data is displayed.
and the “MTC” message.
Use ARROW keys when is displayed to scroll between the records.
If Delete was pressed the instrument will ask for confirmation.
Use the ARROW keys to focus on the record to be deleted.
Press Confirm to delete the record or ESC to return to the previous screen without deleting.
36
Deleting a record will renumber the list of records.
If Delete All was pressed the instrument will ask for confirmation.
Press Confirm to delete all records or ESC to return to the previous screen without deleting.
If the pH log is empty the message “No Records!” will be displayed.
pH METER GLP INFORMATION
The pH meter GLP screens references the last pH calibration data. GLP is an acronym for Good
Laboratory Practice.
To view this information, press MENU key then GLP.
A set of information regarding electrode calibration is displayed.
The following items are included in electrode GLP: the time and date of the last calibration,
offset, slope, calibration timeout and the calibration buffers.
If a calibration hasn’t been performed the message “Not Calibrated” will be displayed.
37
TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only)
All the instruments are factory calibrated for temperature.
HANNA’s temperature probes are interchangeable and no temperature calibration is needed when
they are replaced.
If the temperature measurements are inaccurate, temperature recalibration should be performed.
For an accurate recalibration, contact your dealer or the nearest HANNA Customer Service Center,
or follow the instructions below.
• Prepare a vessel containing ice and water and another one containing hot water (at a
temperature of around 50 °C). Place insulation material around the vessels to minimize
temperature changes.
• Use a calibrated thermometer with a resolution of 0.1 °C as a reference.
• To enter user calibration screen press and hold down the ARROW keys simultaneously, then
power on the instrument. After a few seconds the User calibration screen is displayed.
• Press Temp function key to enter temperature calibration.
• Immerse the temperature probe in the vessel with ice and water as near as possible to the
reference thermometer. Allow a few seconds for the probe to stabilize.
• Use the ARROW keys to set the calibration point value to that of the ice and water measured
by the reference thermometer.
• The
• When the reading is stable and close to the selected calibration point, the
(unstable measurement) symbol will be shown on the display until the reading
becomes stable.
(unstable
measurement) symbol will disappear and the Confirm key will become active.
• Press Confirm to confirm the calibration point.
• The meter will be automatically move to the second calibration point, and will display 50 °C
for the buffer value.
38
• Immerse the temperature probe in the second vessel as near as possible to the reference
thermometer. Allow a few seconds for the probe to stabilize.
• Use the ARROW keys to set the calibration point value to that of the hot water, measured by
the reference thermometer.
• The
• When the reading is stable and close to the selected calibration point, the
• Press Confirm to confirm the calibration point. The instrument will return to the pH meter/
Note: If the reading is not close to the selected calibration point, the “Wrong” message will be
(unstable measurement) symbol will be shown on the display until the reading
becomes stable.
(unstable
measurement) symbol will disappear and the Confirm key will become active.
titrator main screen.
displayed. Change the temperature probe and restart the calibration.
If the temperature probe is disconnected or the measured temperature is out of the - 20 to120 °C
range the instrument will display “----”. The calibration point value can be changed using the
ARROW keys.
39
TROUBLESHOOTING GUIDE
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40
ELECTRODE CONDITIONING AND MAINTENANCE
STORAGE PROCEDURE
To assure a quick response time, the glass bulb should be kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage
Solution. Follow the Preparation Procedure section before taking measurements.
Note: NEVER STORE THE pH ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE
Inspect the electrodes and the cables. The cable used for connection to the instrument must be
intact and there must be no broken insulation on the cable or cracks on the electrode stem or
bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace
the electrode. Rinse off any salt deposits with water.
pH CLEANING PROCEDURE
•
General
IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly
with distilled or deionized water and soak the electrode in HI 70300 or HI 80300 Storage
Solution for at least 1 hour before use.
Soak in Hanna HI 7061 or HI 8061 General Cleaning Solution for
approximately ½ hour.
Recalibrate electrode before takiing measurements.
41
ACCESSORIES
REAGENTS
HI 84432-50 Titrant solution (100mL)
HI 84432-55 Pump calibration solution (100mL)
HI 84432-70 Reagents kit (about 150 titrations)
pH CALIBRATION SOLUTIONS
HI 7004M Buffer solution pH 4.01 (230mL)
HI 7007M Buffer solution pH 7.01 (230mL)
HI 70082M Buffer solution pH 8.20 (230mL)
HI 7004M-6 Buffer solution pH 4.01 (6 x 230mL)
HI 7007M-6 Buffer solution pH 7.01 (6 x 230mL)
HI 70082M-6 Buffer solution pH 8.20 (6 x 230mL)
ELECTRODES
HI 1131B pH Electrode
HI 7662-M Temperature probe
ELECTRODE FILL SOLUTION
HI 7071 Filling solution for HI 1131B (4x30 mL)
ELECTRODE STORAGE SOLUTION
HI 70300M Storage Solution, 230 mL bottle
CLEANING SOLUTION
HI 7061M Electrode Cleaning Solution, 230 mL bottle
OTHER ACCESSORIES
HI 70483M Tube set with cap and tip for titrant bottle
HI 731319 Stir bar 25 x 7 mm (10 pcs)
HI 731342 2000μL fixed - volume pipette for automatic dosage
HI 731352 Tip for 2000μL fixed - volume pipette (4pcs.)
HI 731341 1000μL fixed - volume pipette for automatic dosage
HI 731351 Tip for 1000μL fixed - volume pipette (25pcs.)
42
WARRANTY
HI 84432 is warranted for two years against defects in workmanship and materials when used for its
intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of purchase,
serial number and the nature of the failure. If the repair is not covered by the warranty, you will be
notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid. When
shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of
purchase.
RECOMMENDATION FOR USERS
Before using this product, make sure that it is entirely suitable for your specific application and for the
environment in which it is used.
Operation of this instrument may cause unacceptable interferences to other electronic equipments, this
requiring the operator to take all necessary steps to correct interferences.
Any variation introduced by the user to the supplied equipment may degrade the instrument EMC
performance.
To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the instrument
safety do not use or store the instrument in hazardous environments.
Hanna Instruments reserves the right to modify the design, construction and appearance of its
products without advance notice.
43
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
Local Sales and Customer Service office
Hanna Instruments United States Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
Tel. (800) 426 6287
Fax (401) 765 7575
www.hannainst.com/usa
Technical Support for customers
Telephone (800) 426 6287
Fax (401) 765 7575
E-mail tech@hannainst.com
Printed in Romania, EU
MAN84432 07/09
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