Hanna Instruments HI 84185 User Manual

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Instruction Manual

HI 84185

ISE Ammonia Nitrogen

Meter

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.

This instrument is in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION................................................................................................. 3

GENERAL DESCRIPTION ........................................................................................................ 4

SPECIFICATIONS................................................................................................................... 5

PRINCIPLE OF OPERATION .................................................................................................... 6

FUNCTIONAL DESCRIPTION................................................................................................... 7

START UP ............................................................................................................................ 8

GUIDE TO DISPLAY CODES ..................................................................................................... 9

TIPS FOR AN ACCURATE MEASUREMENT ............................................................................. 16

MEASUREMENT PROCEDURE .............................................................................................. 16

FUSE REPLACEMENT .......................................................................................................... 18

ELECTRODE CONDITIONING AND MAINTENANCE .................................................................. 18

ACCESSORIES .....................................................................................................................22

WARRANTY........................................................................................................................23

MODEL IDENTIFICATION

Two models are available:

HI 84185-01 - 115 Vac power supply HI 84185-02 - 230 Vac power supply

WARRA (NTY

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer.

Each HI 84185 instrument is supplied complete with:

• Reagents set for 20 tests

• One 1000 µL automatic pipette

• Six plastic tips for the 1000 µL automatic pipette

• Two 50 mL beakers

• HI 61101 Ammonia electrode

• HI 7662-T Temperature probe

• Stir bar (2 pcs.)

• Power cable

• HI 4001-40 Refilling solution (4×30 mL)

• HI 4001-51 Replacement membrane (10 pcs.)

• One HI 4000-47-4 and one HI 4000-47-7 pH 4 & 7 powder packets

• One 1 mL syringe

• One 1 mL pipette

• Instruction sheet

• Instruction manual

• One pair of tweezers

Note:Save all packing material until you are sure that the instrument works correctly.

Any defective item must be returned in its original packing.

GENERAL DESCRIPTION

The HI 84185 is a low cost, easy to use, ammonia nitrogen (N-NH

) ISE instrument that performs

automatic analysis with all the necessary calculations assuring to the user a simple and effective interface.

The instrument comes with a powerful and effective built-in algorithm to analyse the shape of the ISE electrode response and to determine the reaction completion.

By simply pressing the Start key, the instrument performs automatic analysis, all the necessary calculations and verifications. The result is immediately displayed in convenient units, then the instrument is ready for another measurement.

SIGNIFICANCE OF USE

The nitrogenous compounds of must and wine are deriving from grapes and play important role in fermentation, clarification, and potential microbial instability of wines. They are profoundly modified during the alcoholic fermentation by the physiologic activity of yeast. Thus, yeast assimilates 60-70% of the must nitrogen, ammonium ion completely disappearing during the fermentation and the total nitrogen being slightly reduced.

Ammonia is present in grapes as ammonium ion in a few milligrams amount and it serves as the primary form of available nitrogen for yeast metabolism. So, the content of ammonium ion can drastically decrease during the alcoholic fermentation, increasing again, especially in red wines, at the end of the malolactic fermentation because the lactic bacteria release ammonia nitrogen in wine.

The amount of ammonium ion in must influences the rapidity of fermentation start and evolution.

The ammonia concentration ranges from 24 to 209 mg/L (ppm) in grapes and from a few mg/L (ppm) to about 50 mg/L (ppm) in wine.

The HI 84185 ISE Ammonia Nitrogen meter measures the ammonia nitrogen (N-NH

) content in

wine using an ion selective electrode. The used method is double standard addition, a simple and rapid method of analysis, and the result is readily displayed in ammonia nitrogen (N-NH3) mg/L (ppm).

SPECIFICATIONS

Range 0 to 50 mg/L (ppm) N-NH Resolution 1 mg/L (ppm) N-NH

Accuracy ±5% of reading Sample volume 50 mL Temperature Compensation Automatic from 0 to 80 °C Electrode HI 61101 Ammonia combined ISE Temperature Probe HI 7662-T (included) LOG feature 50 samples Stirring speed 500 rpm Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing Power supply 220V/50Hz; 10VA Dimensions 208 x 214 x 163 mm (8.2 x 8.4 x 6.4") (with beaker) Weight 2200 g (77.6 oz.)

REQUIRED REAGENTS Code Description Quantity/Test HI 84185-0 ISA alkaline reagent 1 mL HI 84185-1 Standard 1 1 mL HI 84185-2 Standard 2 1 mL

PRINCIPLE OF OPERATION

The determination of ammonia nitrogen concentration is made according to double standard addition method. This technique enables an unknown concentration and the electrode’s slope factor to be determined simultaneously.

The Automatic Temperature compensation is available using HI 7662-T temperature probe. By setting the electrode isopotential point and enabling temperature compensation option in Setup menu, the obtained concentration is temperature compensated.

The accuracy of the obtained result depends on the stability of the mV reading in every step of the measurement.

Three selectable stability criteria can be selected in Setup menu. Select Medium or Accurate criteria in order to have accurate analysis.

FUNCTIONAL DESCRIPTION

INSTRUMENT DESCRIPTION

FRONT PANEL

1) Graphic LCD

2) Functional keys

3) Keypad

4) Electrode holder

REAR PANEL

5) Ammonia electrode 10) BNC electrode connector

6) Temperature probe 11) Power switch

7) Electrode holder 12) Fuse

8) Reference socket 13) Power cable connector

9) Temperature probe socket

KEYPAD DESCRIPTION

1) ESC - to leave the current screen and to return either to the previous screen or to the main screen. In the setup parameter modifying mode by pressing ESC the value is not modified.

3) HELP - to access/leave the instrument’s contextual help

5) Functional Keys

- to modify a parameter value, to scroll the information from the instrument’s setup

START UP

• Place the instrument on a flat table. Do not place the instrument in direct sunlight.

• Connect the instrument to mains socket with ground connection and the correct voltage and

frequency. See the label on the instrument rear for this.

• Connect the HI 61101 ammonia electrode and the HI 7662-T temperature probe.

• Turn the instrument ON using the power switch from the rear panel of the instrument and

wait until main screen is displayed.

GUIDE TO DISPLAY CODES

During the instrument’s operation a series of informations are displayed on the LCD.

1) current time information area

2) instrument status area

3) functional keys area

4) stability criteria

5) stirrer and reading status area

Icons from the stirrer and reading status area

When the input reading is unstable. When the stirrer is on. Temperature warning (only if the “Temperature compensation” is disabled). The

warning symbol will be displayed if the temperature difference between the temperature of the sample confirmed at the first step and the subsequent readings is greater than 3 °C.

Measure screens

Main screen display

Press Setup to acces the instrument’s setup. Press RCL to view logged data. Press Start to begin a new measurement cycle. Press HELP at any moment to view the contextual help.

Conditioning screen

A two minutes conditioning period is initiated. Press Continue to stop this conditioning period and skip the

message.

Measure screen

Press ESC at any moment during the measurement cycle and the instrument will return to the main screen.

Press Pause to pause the measurement process. Press Stir to start/stop the stirrer.

If the difference between the temperature of the sample confirmed at the first step and the subsequent readings is greater than 3°C and the temperature compensation feature is disabled in Setup, the temperature warning icon will blink and the message “WARNING: Large temperature change!” will be displayed.

Pause screen

Press Resume to continue the measurement process. Press Stir to start/stop the stirrer.

Sample reading confirm screen (only if this feature is enabled in setup)

Press CFM to memorize the reading.

Wrong input error screen

This screen is displayed if the current reading is out of ±2000mV range.

Wrong standard error screen

This screen is displayed if step 2 and 3 of the current reading is out of ±2000mV range or if the difference between the stable readings from the current and the previous step is to small (±3mV) or, if in auto confirm mode, the standard addition is not detected.

Add standard screen

Add the corresponding standard. Press Read to start the next step analysis if “Reading confirm” option is enabled in the Setup menu. If “Reading confirm” option is disabled, the instrument will start with the next step analysis.

Result screen

The ion concentration is displayed in mg/L (ppm) N-NH

. If the

concentration is out of range, the instrument displays the upper range limit blinking.

Press LOG to save the measured concentration. A message indicating the free log space will be displayed for a few seconds. If the free log space is under 10% this message will be permanently displayed.

Press Start to begin a new measurement cycle.

Wrong slope screen

This screen appears if the computed slope is not in the acceptable range. The acceptable range is 30% to 140% default slope (-56mV/decade).

Press Start to begin a new measurement cycle.

Setup screens

While in the Setup menu it is possible to modify the instrument’s parameters. By pressing the

/ keys it is possible to move between the Setup options. Each menu item displays the

current option. Press HELP to view the contextual help.

Stability criteria

Options: Accurate, Medium, Fast. Press the corresponding functional key to select the desired option. Select Accurate for accurate measurements; long duration.

Select Medium for normal measurements. Select Fast only for quick estimations; low accuracy in short time.

Temperature compensation

Options: Enable, Disable Enable this feature only if HI 7662-T temperature probe is

connected and electrode isopotential point is known. If enabled, the meter will take into account the temperature

of the readings when evaluating the final result. If disabled, the computed concentration will not be temperature

compensated. Press the functional key to select the desired option.

Isopotential point

Options: 1.0 to 50.0 mg/L (ppm). Press Modify to access the isopotential point concentration

modify screen. Press

/ to increase/decrease the isopotential point

concentration. Press Accept to confirm the value or ESC to return to the

Setup menu without saving. If the temperature compensation feature is disabled the

meter will display “None” for the isopotential point, and the isopotential point modify screen cannot be accessed.

Concentration unit

Options: mg/L, ppm. Press the corresponding functional key to select the desired concentration unit to be used in displaying measurements results.

Backlight

Options: On-Off, 1 to 8 levels. Press Modify to access the backlight modify screen.

Press

or / in order to increase/decrease the display’s

backlight intensity. Press Accept to confirm the value or ESC to return to the

Setup menu without saving.

Contrast

Options: 0 to 20. Press Modify to access the contrast value modify screen.

Press

or /

in order to increase/decrease the

display’s contrast. Press Accept to confirm the value or ESC to return to Setup

menu without saving.

Date/Time

This option is used to set the instrument’s date and time. Press Modify to access the date/time modify screen.

Date/Time modify screen

Press

to select the value to be modified (year, month, day, hour, minute or second) and then press / in order to increase/decrease the value.

Press Accept to confirm the new values or ESC to return to

Setup menu without saving.

Time format

Options: AM/PM or 24 hours. Press the functional key in order to change the time format.

Date format

Press Modify to access the date format modify screen.

Press / or in order to select the desired date format. Press Accept to confirm the value or ESC to return to the

Setup menu without saving.

Language

Options: Up to four languages This option is used to change the instrument’s display language. Press the corresponding functional key in order to select the

new option. If the new selected language cannot be loaded, the previously selected language will be kept.

The HELP

information is not available if no language file can be loaded.

Beep status

Options: Enable, Disable This option enables/disables the beep feature. Press the functional key in order to select the new option. When enabled, beep sounds as a short beep every time a key

is pressed or when the reading must be confirmed. In auto confirm mode the instrument will beep until the standard addition is detected. A long beep alerts that the pressed key is not active.

Reading confirm

Option: Enable, Disable Enable this feature to manually confirm a stable reading. If disabled, the instrument will automatically store a reading when it becomes stable, and move to the next step of the measurement cycle. Press the functional key in order to select the new option.

Meter information

Press Select to view the firmware version, language version, mV factory calibration date and time and temperature factory calibration date and time.

Recall screens

Displays the list of all the memorized results. This list includes the record’s number, the concentration value, the concentration unit and the date.

Press

/ to toggle between the stored records.

Press More to view a complete set of data corresponding to the selected record. To return to the previous screen press ESC.

/ to toggle between complete record information

Press screens.

Press Delete to erase the currently selected record from the instrument’s memory. After pressing Delete the instrument will ask for confirmation. Press CFM to delete the record or ESC to return to the previous screen without deleting.

Deleting a record will reorganize the list of records.

Press Delete All to erase all the stored records. After pressing

Delete All the instrument will ask for confirmation. Press CFM to delete all the records or ESC to return to the previous

screen without deleting.

If the log is empty the message “No Records!” will be displayed.

TIPS FOR AN ACCURATE MEASUREMENT

The instructions listed bellow should be carefully followed during analysis to ensure best accuracy.

• Rinse the electrode with distilled or deionized water between samples, and blot dry taking care not to touch de membrane.

• Select the adequate stability criteria.

• Analyse the wine sample at room temperature.

MEASUREMENT PROCEDURE

• Fill the beaker up to the 50 mL mark with sample, place the stir bar into the beaker and then place the beaker in the appropriate place on the instrument top.

• Place the electrode holder on the top of the beaker and secure it by turning clockwise. Close all unused holes of the beaker holder.

• Immerse HI 61101 ammonia electrode and the HI 7662-T temperature probe approximately 2 cm (0.8“) into the sample to be tested paying attention to not touch the stir bar, and press Start.

• Use the 1000 µL automatic pipette to add exactly 1 mL HI 84185-0 ISA and immediately close the stopper.

• Wait until the reading is stable, CFM key displayed, if the “Reading confirm” option is selected, and then press CFM. If the “Reading confirm” option is disabled the instrument automatically memorizes the reading when this is stable and waits to add standard. Until the standard addition is detected the instrument will beep if beeper is enabled in Setup.

• Use the 1000 µL automatic pipette to add exactly

1 mL of HI 84185-1 Standard and immediatly close the stopper.

• Use the 1000 µL automatic pipette to add exactly 1 mL of HI 84185-2 Standard and immediatly close the stopper.

• At the end of the measurement procedure the result is displayed as mg/L (ppm) N-NH

Notes:

• If the reading is out of range, the upper range value is displayed blinking.

• If the evaluated slope is out of 30-140 % range the instrument displays “- - - -” and “Wrong slope” message.

• If auto confirm mode is selected and beep feature is enabled, the instrument will beep after reading is confirmed until the addition of the standard is detected. If the adding of the standard is not detected after a period of time between 10 to 20 minutes, in according with the stability criteria, “Wrong standard” message will be displayed.

FUSE REPLACEMENT

To change the fuse follow next steps:

• Disconnect the power cord from the rear panel of the instrument.

• Pull out the fuse holder located near the power cord connector.

• Replace the fuse with a similar one.

• Push the fuse holder with the fuse in the appropriate place.

ELECTRODE CONDITIONING & MAINTENANCE

INNER ELECTRODE CHECK

• Before assembling the electrode for the first time or reactivating it after storage, the inner electrode assembly should be hydrated then tested as a pH electrode.

• Take two beakers and pour 50 mL de-ionized water in each of them.

• Add one powder packet of HI 4000-47-4 pH 4 in one of the beakers.

• Add one powder packet of HI 4000-47-7 pH 7 in the other beaker.

• Remove the protective shipping cap from the glass inner electrode. Handle the sensor by the black cap, and use with care to prevent breaking the glass.

• Or unscrew the upper cap on the top of the electrode and carefully withdraw the internal glass assembly. Do not touch the sensing portion with your fingers.

• If the sensor has been stored/shipped dry, it should be “conditioned” by soaking the pH glass in a small sample of one of the buffers, prepared above, for 1 hour or longer. Immerse the glass tip in small sample of the buffer while supporting the upper portion in an electrode holder. Take care that the glass is fragile.

• Connect the BNC connector on the cable assembly to a pH/mV (ORP mode) meter. Carefully immerse the sensor tip into the buffer. Record the mV in each solution once the values have stopped changing. Pay attention to minus sign if it is present.

• Rinse tip in deionized water and blot dry between buffers.

• Calculate the difference in mV between the two solutions.

• The ideal difference is 175 mV. Values greater than 180 mV are acceptable (90% efficiency).

PREPARATION PROCEDURE

• Hold the probe vertically, unscrew the top cap and remove the glass electrode inner body from the electrode outer body.

• Perform the inner electrode check.

• Unscrew membrane cap from outer body and install membrane using tweezers. Avoid touching working area of membrane with your fingers as skin oil will change the hydrophobic properties of the membrane. Discard paper backing between membranes. Drape membrane over lower opening of outer body and hold one corner against the threads. Smooth excess membrane material around the threads.

• Screw outer membrane cap onto body thus capturing the membrane between the cap and outer body thread.

• Add 2 mL of Ammonia internal electrolyte HI 4001-40 into outer probe body.

• Insert and position the internal glass part into the outer body so that the anti-rotation key sits in the cutout on the outer probe body.

• Slide electrode cap and spring down cable and screw top cap onto outer body until fully engaged. Afterwards, unscrew top cap two times. Do not invert electrode. Keep the membrane side down.

• Gently pull spring loaded cable back and slowly release to allow filling solution to migrate between membrane and glass electrode inner body. Screw top cap onto outer body until fully engaged.

• Install assembled electrode in gas sensor test vessel or in electrode holder and connect cable connector to the meter.

SAMPLE HANDLING

• Keep samples stored in tightly converted bottles to prevent ammonia loss or ammonia contamination from other sources.

• Alkaline samples must be measured at once or acidified for storage. (HCl may be added to bring pH to 6).

• Acidic samples such as wine or juice may require additional ISA quantity because the pH of the sample should be arround 11 for measurement.

• Measure samples and standards quickly after adding ISA because ammonia gas will escape from the solution.

• For solution containing oil, sluge and waste samples (which may contain surfactants), digest sample first using a total Kjeldahl nitrogen (TKN) procedure.

• For samples found to penetrate or “wet” the membrane. Measurements may be made above the sample in a small headspace of a closed system (flask). The system should be saturated with water vapor, the membrane tip suspended in the vapor phase above the sample, and the concentration of N-NH

greater than 10-3M. In this case a loger response time is expected.

• Reuse of standards with already added ISA is not advised, because loss of ammonia is expected.

CLEANING PROCEDURE

If the sensor slope just misses the suggested slope window, soaking the sensor in a standard for a

-2

period of time may solve the problem (choose 10

M or 1 000 ppm Ammonia standard).

Rinse in deionised water and blot dry.

QUICK CHECK OF THE ELECTRODE SLOPE

• Connect the electrode and the reference electrode to the instrument.

• Press and hold down ESC,

until the instrument enters in the reading check mode.

• Fill a beaker with 50 mL of deionized water and put a stir bar inside. Place the beaker in its appropriate place on the instrument top.

• Put the electrode holder and insert the electrode and temperature probe in the solution. Add 2 mL of HI 84185-0 ISA buffer. Close all holes.

• Add rapidly 1 mL of HI 84185-1 Standard taking care to close all holes immediately after addition. Wait for the reading to stabilize and record the mV value.

• Add rapidly 10 mL of HI 84185-1 Standard like before taking care to close all holes immediately after addition. Wait for the reading to stabilize and record the mV value.

• The difference between the two recorded mV values gives the slope of the electrode. An accepted value for this slope is -56±4mV.

• If sensor does not change mV the internal glass may have broken.

• Press ESC to leave the reading check mode.

PERIODIC MAINTENANCE

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable, or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse electrode with deionized water between samples and blot dry with lab wipe or other soft disposable absorbent towelling.

and HELP keys simultaneously and power on the meter. Wait

STORAGE PROCEDURE

The HI 61101 probe can be stored assembled in HI 4001-45 Ammonia Conditioning and Storage solution overnight or short term. Gently pull on the cable to compress the spring mechanism thus permitting electrolyte to exchange from the bulk to the thin film between the membrane and glass before resuming use. For storage over a week, disassemble the sensor completely and rinse off the inner pH/ref assembly, the outer body and the membrane cap. Discard membrane. Cover the glass tip with the protective cover used in shipment and store parts securely in the original shipping box. When reassembling the sensor follow the Preparation Procedure section.

MALFUNCTIONING

• No slope (meter always reads the same value): Repeat the measurement with a fresh ammonia electrode.

ACCESSORIES

REAGENT SETS HI 4001-40 Ammonia filling solution HI 4001-45 Ammonia conditioning and storage solution HI 4000-47 Buffer replacement kit HI 84185-20 Reagent set HI 84185-0 ISA for Ammonia electrode (500 mL) HI 84185-1 Standard 1 for Ammonia electrode (500 mL) HI 85185-2 Standard 2 for Ammonia electrode (500 mL)

OTHER ACCESSORIES HI 61001-51 Membrane (20 pcs.) HI 61101 Ammonia electrode HI 731316 Stir bar (5 pcs.) HI 731341 Automatic pipette 1000 µL HI 731351 Tips for 1000 µL automatic pipette (25 pcs.) HI 731355P Capilary pipette (20 pcs.) HI 740036P Plastic beaker 50 mL (10 pcs.) HI 70143 Syringe 1mL (6 pcs.) HI 740144 Syringe tip (6 pcs.) HI 7662-T Temperature probe

WARRANTY

HI 84185 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of

purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.

Recommendations for Users

Before using this product, make sure that it is entirely suitable for your specific application and for the environment in which it is used.

Operation of this instrument may cause unacceptable interferences to other electronic equipments, this requiring the operator to take all necessary steps to correct interferences.

Any variation introduced by the user to the supplied equipment may degrade the instrument EMC performance. To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the instrument’s safety do not use or store the instrument in hazardous environments.

WARRA (NTY

Hanna Instruments reserves the right to modify the design, construction and appearance of its products

without advance notice.

SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS

SALES AND TECHNICAL SERVICE CONTACTS

SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS

Australia:

Tel. (03) 9769.0666 • Fax (03) 9769.0699

China:

Tel. (10) 88570068 • Fax (10) 88570060

Egypt:

Tel. & Fax (02) 2758.683

Germany:

Tel. (07851) 9129-0 • Fax (07851) 9129-99

Greece:

Tel. (210) 823.5192 • Fax (210) 884.0210

Indonesia:

Tel. (210) 4584.2941 • Fax (210) 4584.2942

Japan:

Tel. (03) 3258.9565 • Fax (03) 3258.9567

Korea:

Tel. (02) 2278.5147 • Fax (02) 2264.1729

Malaysia:

Tel. (603) 5638.9940 • Fax (603) 5638.9829

Singapore:

Tel. 6296.7118 • Fax 6291.6906

South Africa:

Tel. (011) 615.6076 • Fax (011) 615.8582

Taiwan:

Tel. 886.2.2739.3014 • Fax 886.2.2739.2983

Thailand:

Tel. 66.2619.0708 • Fax 66.2619.0061

10/06

United Kingdom:

Tel. (01525) 850.855 • Fax (01525) 853.668

USA:

Tel. (401) 765.7500 • Fax (401) 765.7575

For e-mail contacts and a complete list of Sales and Technical offices, please see

www.hannainst.com

MAN84185

Hanna Instruments HI 84185 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual