Hanna Instruments HI 84185 User Manual

Instruction Manual

HI 84185

ISE Ammonia Nitrogen

Meter

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.

This instrument is in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION................................................................................................. 3

GENERAL DESCRIPTION ........................................................................................................ 4

SPECIFICATIONS................................................................................................................... 5

PRINCIPLE OF OPERATION .................................................................................................... 6

FUNCTIONAL DESCRIPTION................................................................................................... 7

START UP ............................................................................................................................ 8

GUIDE TO DISPLAY CODES ..................................................................................................... 9

TIPS FOR AN ACCURATE MEASUREMENT ............................................................................. 16

MEASUREMENT PROCEDURE .............................................................................................. 16

FUSE REPLACEMENT .......................................................................................................... 18

ELECTRODE CONDITIONING AND MAINTENANCE .................................................................. 18

ACCESSORIES .....................................................................................................................22

WARRANTY........................................................................................................................23

MODEL IDENTIFICATION

Two models are available:

HI 84185-01 - 115 Vac power supply HI 84185-02 - 230 Vac power supply

WARRA (NTY

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer.

Each HI 84185 instrument is supplied complete with:

• Reagents set for 20 tests

• One 1000 µL automatic pipette

• Six plastic tips for the 1000 µL automatic pipette

• Two 50 mL beakers

• HI 61101 Ammonia electrode

• HI 7662-T Temperature probe

• Stir bar (2 pcs.)

• Power cable

• HI 4001-40 Refilling solution (4×30 mL)

• HI 4001-51 Replacement membrane (10 pcs.)

• One HI 4000-47-4 and one HI 4000-47-7 pH 4 & 7 powder packets

• One 1 mL syringe

• One 1 mL pipette

• Instruction sheet

• Instruction manual

• One pair of tweezers

Note:Save all packing material until you are sure that the instrument works correctly.

Any defective item must be returned in its original packing.

GENERAL DESCRIPTION

The HI 84185 is a low cost, easy to use, ammonia nitrogen (N-NH

) ISE instrument that performs

automatic analysis with all the necessary calculations assuring to the user a simple and effective interface.

The instrument comes with a powerful and effective built-in algorithm to analyse the shape of the ISE electrode response and to determine the reaction completion.

By simply pressing the Start key, the instrument performs automatic analysis, all the necessary calculations and verifications. The result is immediately displayed in convenient units, then the instrument is ready for another measurement.

SIGNIFICANCE OF USE

The nitrogenous compounds of must and wine are deriving from grapes and play important role in fermentation, clarification, and potential microbial instability of wines. They are profoundly modified during the alcoholic fermentation by the physiologic activity of yeast. Thus, yeast assimilates 60-70% of the must nitrogen, ammonium ion completely disappearing during the fermentation and the total nitrogen being slightly reduced.

Ammonia is present in grapes as ammonium ion in a few milligrams amount and it serves as the primary form of available nitrogen for yeast metabolism. So, the content of ammonium ion can drastically decrease during the alcoholic fermentation, increasing again, especially in red wines, at the end of the malolactic fermentation because the lactic bacteria release ammonia nitrogen in wine.

The amount of ammonium ion in must influences the rapidity of fermentation start and evolution.

The ammonia concentration ranges from 24 to 209 mg/L (ppm) in grapes and from a few mg/L (ppm) to about 50 mg/L (ppm) in wine.

The HI 84185 ISE Ammonia Nitrogen meter measures the ammonia nitrogen (N-NH

) content in

wine using an ion selective electrode. The used method is double standard addition, a simple and rapid method of analysis, and the result is readily displayed in ammonia nitrogen (N-NH3) mg/L (ppm).

SPECIFICATIONS

Range 0 to 50 mg/L (ppm) N-NH Resolution 1 mg/L (ppm) N-NH

Accuracy ±5% of reading Sample volume 50 mL Temperature Compensation Automatic from 0 to 80 °C Electrode HI 61101 Ammonia combined ISE Temperature Probe HI 7662-T (included) LOG feature 50 samples Stirring speed 500 rpm Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing Power supply 220V/50Hz; 10VA Dimensions 208 x 214 x 163 mm (8.2 x 8.4 x 6.4") (with beaker) Weight 2200 g (77.6 oz.)

REQUIRED REAGENTS Code Description Quantity/Test HI 84185-0 ISA alkaline reagent 1 mL HI 84185-1 Standard 1 1 mL HI 84185-2 Standard 2 1 mL

PRINCIPLE OF OPERATION

The determination of ammonia nitrogen concentration is made according to double standard addition method. This technique enables an unknown concentration and the electrode’s slope factor to be determined simultaneously.

The Automatic Temperature compensation is available using HI 7662-T temperature probe. By setting the electrode isopotential point and enabling temperature compensation option in Setup menu, the obtained concentration is temperature compensated.

The accuracy of the obtained result depends on the stability of the mV reading in every step of the measurement.

Three selectable stability criteria can be selected in Setup menu. Select Medium or Accurate criteria in order to have accurate analysis.

FUNCTIONAL DESCRIPTION

INSTRUMENT DESCRIPTION

FRONT PANEL

1) Graphic LCD

2) Functional keys

3) Keypad

4) Electrode holder

REAR PANEL

5) Ammonia electrode 10) BNC electrode connector

6) Temperature probe 11) Power switch

7) Electrode holder 12) Fuse

8) Reference socket 13) Power cable connector

9) Temperature probe socket

KEYPAD DESCRIPTION

1) ESC - to leave the current screen and to return either to the previous screen or to the main screen. In the setup parameter modifying mode by pressing ESC the value is not modified.

3) HELP - to access/leave the instrument’s contextual help

5) Functional Keys

- to modify a parameter value, to scroll the information from the instrument’s setup

START UP

• Place the instrument on a flat table. Do not place the instrument in direct sunlight.

• Connect the instrument to mains socket with ground connection and the correct voltage and

frequency. See the label on the instrument rear for this.

• Connect the HI 61101 ammonia electrode and the HI 7662-T temperature probe.

• Turn the instrument ON using the power switch from the rear panel of the instrument and

wait until main screen is displayed.

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