Hanna Instruments HI 83749 User Manual

SALES AND TECHNICAL SERVICE CONTACTS

Australia:

Tel. [phone] • Fax [phone]

Instruction Manual

China:

Tel. [phone] • Fax [phone]

Egypt:

Tel. & Fax [phone]

Germany:

Tel. [phone] • Fax [phone]

Greece:

Tel. [phone] • Fax [phone]

Indonesia:

Tel. [phone] • Fax [phone]

Japan:

Tel. [phone] • Fax [phone]

Korea:

Tel. [phone] • Fax [phone]

Malaysia:

Tel. [phone] • Fax [phone]

Singapore:

Tel. [phone] • Fax [phone]

South Africa:

Tel. [phone] • Fax [phone]

HI 83749

Turbidity & Bentocheck

Taiwan:

Tel. [phone] • Fax [phone]

Thailand:

Tel. [phone] • Fax [phone]

United Kingdom:

Tel. [phone] • Fax [phone]

USA:

Tel. [phone] • Fax [phone]

For e-mail contacts and a complete list of Sales and Technical offices, please see

www.hannainst.com.

11/05

40

MAN83749R1

www.hannainst.com

1

Dear Customer,
Thank you for choosing a Hanna Instruments product. This manual will provide you with the

necessary information for correct use of the instrument.
Please read this instruction manual carefully before using the instrument.
If you need additional technical information, do not hesitate to e-mail us at [e-mail]

or see the back side of this manual for our worldwide sales and technical service contacts.
This instrument is in compliance with

directives.

TABLE OF CONTENTS

WARRANTY ............................................... 2

PRELIMINARY EXAMINATION ...................... 3

GENERAL DESCRIPTION .............................. 4

ABBREVIATIONS ....................................... 5

PRINCIPLE OF OPERATION ......................... 6

MEASUREMENT UNITS............................... 6

SPECIFICATIONS ........................................ 7

FUNCTIONAL DESCRIPTION ........................ 8

GENERAL TIPS FOR AN ACCURATE

MEASUREMENT....................................... 10

CALIBRATION PROCEDURE ....................... 12

TURBIDITY MEASUREMENT ...................... 16

BENTOCHECK (PROTEIN STABILITY TEST) ... 18

DETERMINATION OF BENTONITE

REQUIREMENT ........................................ 20

LOGGING ................................................ 24

GOOD LABORATORY PRACTICE (GLP) ......... 27

SETUP .................................................... 29

LCD BACKLIGHT ....................................... 35

TAG INSTALLATION .................................. 35

LAMP REPLACEMENT ............................... 35

BATTERIES MANAGEMENT ........................ 36

ERROR CODES......................................... 37

PC INTERFACE ......................................... 38

CE DECLARATION OF CONFORMITY ............ 38

ACCESSORIES ......................................... 39

WARRANTY

ACCESSORIES

HI 83749-20 Bentocheck (100 mL)
HI 93703-58 Silicone oil (15 mL)
HI 83749-11 Calibration cuvets kit
HI 731331 Glass cuvets (4 pcs)
HI 731335N Caps for cuvets (4 pcs)
HI 93703-50 Cuvets cleaning solution (230 mL)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 740220 25 mL glass vial with cap (2 pcs)
HI 731341 Automatic pipette 1000 µL
HI 731351 Tips fo automatic pipette 1000 µL (25 pcs)
HI 740233 Filter paper type II (100 pcs)
HI 740142P 1 mL graduated syringe (10 pcs)
HI 740144P Tips for 1 mL syringe (10 pcs)
HI 740234 Replacement lamp for EPA turbidimeter (1 pcs)
HI 92000 Windows® compatible software
HI 920011 RS232 connection cable
HI 920005 5 tag holders with tags
HI 740027P 1.5V AA battery (12 pcs)
HI 710005 Voltage adapter from 115V to 12 VDC (USA plug)
HI 710006 Voltage adapter from 230V to 12 VDC (European plug)
HI 710012 Voltage adapter from 240V to 12 VDC (UK plug)
HI 710013 Voltage adapter from 230V to 12 VDC (South Africa plug)
HI 710014 Voltage adapter from 230V to 12 VDC (Australia plug)

HI 83749 is warranted for two years against defects in workmanship and materials when used for

its intended purpose and maintained according to instructions. This warranty is limited to repair
or replacement free of charge.

Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact the dealer from whom you purchased the instrument. If under

warranty, report the model number, date of purchase, serial number and the nature of the failure.
If the repair is not covered by the warranty, you will be notified of the charges incurred. If the
instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
number from the Technical Service Department and then send it with shipping costs prepaid. When
shipping any instrument, make sure it is properly packed for complete protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.

2

Hanna Instruments reserves the right to modify the design, construction and appearance of its products without

advance notice.

39

PC INTERFACE

PRELIMINARY EXAMINATION

To fully use the instrument tag identification system function, the measured data has to be
downloaded to a computer. The instrument can use RS232 or USB connection to communicate
with the PC.

When using the RS232 protocol, simply connect a HI 920011 serial cable between the instrument
and the computer.

To use the USB protocol, simply connect a regular USB cable between instrument and PC.
In both cases, the PC must run the HI 92000 application for successful data transfer.

CE DECLARATION OF CONFORMITY

Recommendations for Users

Before using these products, make sure that
they are entirely suitable for your specific
application and for the environment in which
they are used.

Operation of these instruments may cause
unacceptable interferences to other electronic
equipments, this requiring the operator to take
all necessary steps to correct interferences.

Any variation introduced by the user to the
supplied equipment may degrade the
instruments' EMC performance.

To avoid damages or burns, do not put the
instrument in microwave ovens. For yours and
the instrument safety do not use or store the
instrument in hazardous environments.

Please examine this product carefully. Make sure the instrument is not damaged. If any damage
has occurred during the shipment, please notify your dealer.

This HI 83749 Turbidity & Bentocheck Meter is supplied complete with:

• Six Sample Cuvets and Caps

• Four Calibration Cuvets (HI 83749-11)

• Bentocheck Reagent (HI 83749-0) and Silicone Oil (HI 93703-58)

• One 1000 µL Automatic Pipette with two Tips and Instructions Sheet

• Four 25 mL Glass Vials with Caps

• One 1 mL Syringe with two Tips; one Funnel; Filter Paper (25 pieces).

• Five Tag Holders with Tags (HI 920005)

• Tissue for wiping the cuvets

• Four 1,5V AA Batteries

• AC Adapter

• Instruction Manual

• Instrument Quality Certificate

• Rigid carrying case

38

Note:Save all packing material until you are sure that the instrument works correctly. Any

defective item must be returned in the original packing with the supplied accessories.

3

GENERAL DESCRIPTION

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GENERAL DESCRIPTION

The HI 83749 is an auto diagnostic microprocessor meter that benefits from Hanna’s years of experience
as manufacturer of analytical instruments.
The meter is especially designed for wine analyses and, beside turbidity measurements, it allows to
make tests to verify protein stability (e.g. Bentocheck).
The instrument compensates wine color to guarantee accurate readings during the vinification process;
also for the darkest red wine samples. The optical system, consisting of a tungsten filament lamp and
multiple detectors, assures long term stability and minimizes the need of frequent calibration. Calibration
can be anyway easily performed at any time in two, three or four points (<0.1, 15, 100 and 500 NTU­adjustable calibration points), using the supplied or user prepared standards.
The meter has all necessary GLP (Good Laboratory Practice) functions to allow maximum traceability
of data like a real time clock, log-on-demand (up to 200 measurements), and T.I.S. -Tag Identification
System to give all recorded data a location, time and date stamp.

The meter measures turbidity of samples from 0.00 to 1200 NTU (Nephelometric Turbidity Units) and
is USEPA compliant. In the USEPA measurement mode the instrument rounds the readings to meet
USEPA reporting requirements. It has a continuous measurement mode to verify the settling rate of
suspended matter, and a signal average (AVG) mode to accumulate multiple readings giving a final
average value. The AVG routine is particularly useful to measure samples with suspended particles
with different dimensions.
The HI 83749 has a user-friendly interface, with a large backlit LCD (Liquid Crystal Display). Acoustic
signals and displayed codes to guide the user step by step through routine operations.
This valuable splash proof portable turbidity meter is supplied in a rigid carrying case that offers
protection for harsh environments.

BENTOCHECK

The prevention of protein haze or deposit in bottled white wines is a universal concern and often a
wine needs to be stabilized before bottling. One commonly used stabilization agent is bentonite.
Bentonite is a volcanic clay earth type fining agent (like kaolin). It improves the clarity and stability of
wine but has also negative aspects because of the volume of lees formed, reduction of tannin and
color. Since there are different types and qualities of bentonite with different capacity of protein
removal, it is important to make laboratory trails with the same lot and wetting degree of the bentonite
as that will be used in the cellar.
Protein stabilization is normally not a problem in bottled red wines because of the relatively high
concentration of phenols that binds and precipitates with the instable proteins before bottling. Often
bentonite is added to red wines at a level of about 12 g/hL (1 lb/1000 gal), reducing colloidal
suspended particles thus improving membrane filterability.

Wines with low phenols contents, such as rose, light reds and whites should be checked for protein
stability before bottling. Hanna is offering a rapid test to verify the risk of future protein haze formation.

4

USING AN AC ADAPTER

In laboratory you can use an AC adapter to power the HI 83749. Simply connect the AC adapter to the
instrument (see Connectors Description, page 9).
It is not necessary to turn the instrument off when connecting the external adapter.
Note: The connection to the external adapter will not recharge the batteries.

ERROR CODES

HI 83749 has a powerful diagnostic system. Common errors are detected and reported for easy
diagnostic and maintenance.

37

BATTERIES MANAGEMENT

For field measurements, HI 83749 is powered by 4 AA batteries.
The battery life is enough for 1500 normal measurements.

When you turn the instrument on, the remaining battery life is
estimated and reported in percents.

To save the battery life, the instrument will turn off after 15
minutes of non-use. The backlight will be turn off after 25
seconds since the last key was pressed.

The battery life is measured each time the lamp is turned on
and if the remaining battery life is less than 10%, the battery
tag will blink on the LCD to advise the user to replace the
batteries.

When the batteries are completely discharged, “0% bAtt” message
will be displayed for one second and the instrument will turn off.

In order to use the instrument again, replace the batteries or
use an AC adapter.

BATTERIES REPLACEMENT

To replace the batteries follow the next steps:

• Turn OFF the instrument by pressin ON/OFF.

• Open the batteries cover by pressing the locking clip.

If protein instability is detected, a next test can help defining the right amount of bentonite to be
added for improving protein stability. It is important not to overdose bentonite, avoiding stripping
wine flavour, body, and significant loss of color, especially in young red wines. Moreover, adding only
the necessary amount of bentonite to obtain the desired protein stability is also costs saving.

TAG IDENTIFICATION SYSTEM

Hanna is the first manufacturer of turbidity instruments that has decided to add the unique T .I.S. - Tag
Identification System to portable turbidity meters, to meet the more restrictive needs for traceability
and data management of our clients.

The system is designed for scientific and industrial applications, or to prove during safety audits and
inspections that samples have been truly taken on pre-established locations.

The system is as easy to install as to operate. Just fix the so-called
sites that need to be checked often, and with this the T.I.S. is setup. The tag contains a computer chip
embedded in a durable stainless steel can. It is designed to withstand the harsh environments, indoors
or outdoors. The number of tags that can be installed is practically unlimited, because each tag has a
unique identification code.

Immediately after installation of the tags you can start collecting data. Use the meter to take measurements
and memorize the test result by pressing the Log-on-Demand key. Then, the instrument will ask for the
tag identification. Simply touching the
and authenticate logging, by storing the iButton® serial number, time and date stamp events.

The power of the T.I.S. features resides in the PC application. Download all test data to your PC and
use our HI 92000 Windows® compatible application software for further data management. You can
sort or filter all your collected data on different criteria like on a specific sampling location, parameter,
date and time intervals, or fix range to filter measured values. The data can be plotted in a graph,
exported to other common Windows® applications or printed for reporting purpose.

It is also possible to add new tags later on, thus increasing an already existing database. Each time
the PC software recognizes a not already registered tag, it will ask for a description of the new
sampling location.

iButton® with the matching connector on the meter does identify

iButton® tags near your sampling

• Take out the used batteries and insert 4 new 1.5 AA size batteries, paying attention to the correct
polarity as indicated on the battery compartment.

• Replace the cover and press it until it locks.

Warning: Replace batteries only in a non-hazardous area.

36

ABBREVIATIONS

NTU Nephelometric Turbidity Units
FTU Formazin Turbidity Units
USEPA US Environmental Protection Agency
LCD Liquid Crystal Display

RTC Real Time Clock
T.I.S. Tag Identification System

iButton® is registered Trademark of “MAXIM/DALLAS semiconductor Corp.”
Windows® is registered Trademark of “MICROSOFT Corporation”

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PRINCIPLE OF OPERATION

LCD BACKLIGHT

A light beam that passes through the sample is scattered in all directions. The intensity and pattern of
the scattered light is affected by many variables like wavelenght of the incident light, particle size,
shape, refractive index and color.
The optical system includes a tungsten filament lamp, a scattered light detector (900) and a transmitted
light detector (1800). The microprocessor of the instrument calculates the NTU value from the signals
that reaches the two detectors.

The lower detection limit of a turbidimeter is determined by the so called “stray light”. Stray light is
the light detected by the sensors, that is not caused by light scattering from suspended particles.
The optical system of HI 83749 turbidimeter is designed to have very low stray light, providing
accurate results also for low turbidity samples. However, special care must be taken when measuring
low turbidities (see General Tips for an Accurate Measurement, page 10).

MEASUREMENT UNITS

Many methods were used to measure turbidity over the years. The Jackson Candle Turbidimeter was
used to measure turbidity as Jackson turbidity units (JTU). The Secchi Disk is commonly used to
measure turbidity in lakes and other deep waters (mg/L SiO2). Both methods are visual and are not
considered very accurate. To obtain more accurate readings a nephelometer should be used as a
turbidity reading instrument.

The HI 83749 turbidimeter reports the measurements in NTU (Nephelometric Turbidity Units).
NTU units are equal to FTU units (Formazine Turbidity Units). The conversion table between these
measurement units is shown bellow:

The LCD can be illuminated to allow the user to see the readings even in dark
environments.
To turn on or off the backlight, press the ON/OFF key.
The backlight will automatically shut-off after 25 seconds of non-use to save
the battery life.

TAG INSTALLATION

The tag is housed in a rugged metal that can withstand harsh environments. However, it is better
to protect the tag from direct rain.

Place the tag near a sampling point. Fix it securely with the provided screws, in such a way that
the metallic iButton® is easily accessible for reading the tag.

The number of tags that can be installed is practically unlimited. Additional tags can be ordered

(See Accessories, page...) HI 920005 - five tag holders with tags).

LAMP REPLACEMENT

The instrument tungsten lamp has a life longer than 100,000 measurements. In case of lamp failure,
the defective lamp can be easily replaced. When the lamp is broken, the instrument displays “no L”
error message.
To replace the lamp follow the next steps:

• Remove the battery lid.

• Unscrew the lamp connection using a screwdriver.

• Unlock the lamp and extract it by pulling it out
from the lamp holder handler.

• Place the new lamp in the right position and
push it until is securely locked.

• Insert the lamp leads into the connector and
tight them using a screwdriver.

Warning: After lamp replacement the meter needs

to be recalibrated.

6

35