Hanna Instruments HI 83742 User Manual
Instruction Manual
HI 83742
COLOR & PHENOLS
ISM
for wine analysis
www.hannainst.com
MAN83742R3 04/06
www.hannainst.com
This Instrument is in
Compliance with the CE Directives
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
This instrument is in compliance with
directives.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION............................................................................................... 3
GENERAL DESCRIPTION ...................................................................................................... 4
SPECIFICATIONS ................................................................................................................ 5
PRECISION AND ACCURACY ................................................................................................. 5
PRINCIPLE OF OPERATION ..................................................................................................6
ABBREVIATIONS ................................................................................................................ 7
FUNCTIONAL DESCRIPTION .................................................................................................8
GUIDE TO DISPLAY CODES .................................................................................................. 9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ............................................................. 11
OPERATIONAL GUIDE ....................................................................................................... 13
PARAMETER REFERENCE TABLE......................................................................................... 13
COLOR DENSITY, WHITE WINE .......................................................................................... 14
COLOR DENSITY, RED WINE.............................................................................................. 16
COLOR HUE ..................................................................................................................... 18
TOTAL PHENOLS, WHITE WINE ......................................................................................... 20
TOTAL PHENOLS, RED WINE .............................................................................................23
ACCESSORIES ................................................................................................................. 26
BATTERY REPLACEMENT ................................................................................................... 26
CE DECLARATION OF CONFORMITY .................................................................................... 27
WARRANTY ......................................................................................................................27
CE DECLARATION OF CONFORMITY
Recommendations for Users
Before using these products, make sure that
they are entirely suitable for your specific
application and for the environment in which
they are used.
Operation of these instruments may cause
unacceptable interferences to other electronic
equipments, this requiring the operator to take
all necessary steps to correct interferences.
Any variation introduced by the user to the
supplied equipment may degrade the
instruments' EMC performance.
To avoid damages or burns, do not put the
instrument in microwave ovens. For yours and
the instrument safety do not use or store the
instrument in hazardous environments.
WARRANTY
HI 83742 is warranted for two years against defects in workmanship and materials when used for
its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
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Hanna Instruments reserves the right to modify the design, construction and appearance of its products
without advance notice.
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ACCESSORIES
PRELIMINARY EXAMINATION
REAGENT SETS
HI 83742-20 Phenols reagent set for wine (20 tests)
HI 83742-25 Color reagent set for wine (20 tests) containing HI 83742-0 Wine Solvent 1
HI 83742-27 Color reagent set for wine (20 tests) containing HI 83742-3 Wine Solvent 3
OTHER ACCESSORIES
DEMI-10 Bottle to prepare 10 liters of demineralized water
HI 740027P 1.5V AA batteries (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731321 Glass cuvets (4 pcs)
HI 731325W Caps for cuvets (4 pcs)
HI 93703-50 Cuvets cleaning solution (230 mL)
HI 740226 5 mL graduated syringe
HI 731340 200 µL automatic pipette
HI 731350 Plastic tips for 200 µL automatic pipette (25 pcs)
HI 731342 2000 µL automatic pipette
HI 731352 Plastic tips for 2000 µL automatic pipette (25 pcs)
HI 740157 Plastic refilling pipette (20 pcs)
Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occured during shipment, please notify your Dealer.
Each HI 83742 Ion Selective Meter is supplied complete with:
• Two sample cuvets and caps
• Reagents for 5 tests (HI 83742-O, HI 83742A-O HI 83742B-O, HI 83742C-O)
• One 200 µL automatic pipette with two tips and Instruction Sheet
• One 2000 µL automatic pipette with two tips
• One 5 mL syringe with tip
• One 1 mL plastic pipette
• One 3 mL plastic pipette
• 12 VDC transformer (
• Four 1,5V AA batteries
• Tissue for wiping cuvets
• Instruction manual
• Instrument Quality Certificate
• Rigid carrying case
HI 710005 HI 710005
HI 710005 or
HI 710005 HI 710005
HI 710006HI 710006
HI 710006)
HI 710006HI 710006
BATTERY REPLACEMENT
Battery replacement must only take place in a nonhazardous area.
The blinking “
gets low.
When batteries are completely discharged, “0% bAtt”
will appear and after two seconds the instrument is
switched off.
Remove the battery cover from the bottom of the
instrument and change the old batteries with 4 fresh
1.5V batteries, paying attention to the correct polarity.
Replace the cover.
” will appear when the batteries power
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Note: save all packing material until you are sure that the instrument works correctly. Any defective
item must be returned in its original packing.
3
GENERAL DESCRIPTION
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
The HI 83742 is an auto-diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has an advanced optical system
based on a special tungsten lamp and a narrow band interference filter that allows most accurate
and repeatable readings. All instruments are factory calibrated.
The auto-diagnostic feature of this meter ensures always optimal measurement conditions to
ensure most precise readings. The light level is automatically adjusted each time a zero-measurement
is made, and the temperature of the lamp is controlled to avoid overheating.
SIGNIFICANCE OF USE
Color determination of wine
Analytical techniques have become a valuable tool of modern wine makers. Especially the definition and
the processing techniques to obtain the desired wine color are of key importance. The right decisions taken
during maturation of the grapes, processing, aging and blending, all strongly influence the final result of
wine color.
The color of wine is always read after removal of suspended matter. There are manly two color components
present, yellow and red but also a blue or green hue may appear. The color hue is the ratio between the
yellow color concentrations over the red one, and is an indication about the degree of evolution.
The yellow color in wine comes from the present of tannins (polymers of flavonoid -procyanidins type, and
non-flavonoid phenols) and can be read without dilution. The increase of the yellow-brown color in older
wines is due to aging or oxidation.
The red colors of wines are caused by free anthocyanins, copigments of anthocyanins, and polymerized
phenolic compounds. The color of these pigments is pH dependent and can be intense dark. It is therefore
necessary to dilute the wine sample taking care not to change the original wine pH. Hanna recommends
using the special wine solvent to minimize possible errors due to dilution.
• Press “RANGE” to select the parameter code “P5” for
total phenols, red wine (see page 13).
• Fill an empty cuvet with deionized water to the 10 mL
mark and replace the cap. This is the
• Insert the zero cuvet into the holder and close the lid.
• Press ZERO and “----” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
• Insert the sample (cuvet #2) into the holder and
close the lid.
• Press READ/TIMER and “----” will blink during
measurement.
zero.zero.
zero.
zero.zero.
#1
(Zero)
#2
(Sample)
Phenol determination of wine
Phenolic compounds are important for several reasons since they (i) affect the color of the wine, (ii) have
an astringent taste, (iii) may case pungent odder, (iv) are a source of oxygen reduction, and (v) are sources
of browning substances.
Wine can contain a large variety of phenolic compounds and with traditional analytical techniques it is
difficult to distinguish between total phenols and specific phenols. Although some progress has been made
with HPLC, the most common analyses for total phenols remain the reaction of phenolic substances with
the Folin-Ciocalteu reagent. Other methods like the direct spectrophotometric determinations are less
accurate, because of difference in specific molar absorptivity, and color present of non phenolic substances.
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• The instrument directly displays concentration in g/L
of GAE (gallic acid equivalent) on the Liquid Crystal
Display.
Note:
If the phenol concentration is higher than 5 g/L,
then pre-dilute the wine sample in the following way:
use the automatic pipette to add 2 mL of deionized
water and 2 mL of wine sample. Then follow the normal
sample preparation procedure, using this pre-diluted
sample as red wine sample. In this case the final value
must be multiplied by
2.
25
• Use the 200 µL automatic pipette with a clean
pipette tip to add exactly 0,2 mL of diluted red
wine sample.
• Add 6 drops of HI 83742B-0 Folin & Ciocalteu’s
Reagent to the cuvet.
• Replace the cap and shake gently to mix.
•
Wait for 1 minute and then use the 3 mL pipette to
add HI 83742C-0 Reagent C Carbonate Buffer, to
bring the
volume up to the 10 mL mark.
• Turn the meter ON and press RANGE to select the
parameter code “P5” for total phenols, red wine (see
page 13). Then hold down READ/TIMER for several
seconds. The instrument will show the countdown.
Alternatively, wait for 2 hours.
The instrument gives an acoustic signal to alert the
user that the countdown is finished.
• This is the reacted sample.
x 6
10 mL
#2
(Sample)
The reaction between phenols and the Folin-Ciocalteu reagent involves oxidation of the phenolic groups (ROH) with a mixture of phosphotungstenic acid (H3PW12O40) and phosphomolybdenic acid (H3PMo12O40) to
the quinoid form (R=O). The concomitant reduction of the Folin-Ciocalteu reagent causes a blue color in
the sample that is proportional to the total phenolic content that, in turn, is expressed as g/L of Gallic Acid
Equivalents (GAE).
Another common method to express the phenol content is to report the Folin Index (FI) or Total Phenol
Index. Simply multiply the read value in g/L of GAE times 25, to find the Folin Index value.
SPECIFICATIONS
Light Detector Silicon Photocell
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 4 x 1,5 volt AA batteries / 12 to 20 VDC through voltage adapter
Auto-Shut off
Dimensions 225 x 85 x 80 mm (8.7 x 3.3 x 3.1")
Weight 500 g (17,6 oz.).
After 15' of non-use.
PRECISION AND ACCURACY
Precision is how closely repeated measurements agree
with each other. Precision is usually expressed as
standard deviation (SD). Accuracy is defined as the
nearness of a test result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure explains
these definitions.
For example for phenols: in a laboratory using a
standard solution of 0.350 g/L gallic acid and a
representative lot of reagent, an operator obtained
with a single instrument a standard deviation of
0.015 g/L.
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