Instruction Manual
HI 83742
COLOR & PHENOLS
ISM
for wine analysis
www.hannainst.com
MAN83742R3 04/06
www.hannainst.com
This Instrument is in
Compliance with the CE Directives
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
This instrument is in compliance with
directives.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION............................................................................................... 3
GENERAL DESCRIPTION ...................................................................................................... 4
SPECIFICATIONS ................................................................................................................ 5
PRECISION AND ACCURACY ................................................................................................. 5
PRINCIPLE OF OPERATION ..................................................................................................6
ABBREVIATIONS ................................................................................................................ 7
FUNCTIONAL DESCRIPTION .................................................................................................8
GUIDE TO DISPLAY CODES .................................................................................................. 9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ............................................................. 11
OPERATIONAL GUIDE ....................................................................................................... 13
PARAMETER REFERENCE TABLE......................................................................................... 13
COLOR DENSITY, WHITE WINE .......................................................................................... 14
COLOR DENSITY, RED WINE.............................................................................................. 16
COLOR HUE ..................................................................................................................... 18
TOTAL PHENOLS, WHITE WINE ......................................................................................... 20
TOTAL PHENOLS, RED WINE .............................................................................................23
ACCESSORIES ................................................................................................................. 26
BATTERY REPLACEMENT ................................................................................................... 26
CE DECLARATION OF CONFORMITY .................................................................................... 27
WARRANTY ......................................................................................................................27
CE DECLARATION OF CONFORMITY
Recommendations for Users
Before using these products, make sure that
they are entirely suitable for your specific
application and for the environment in which
they are used.
Operation of these instruments may cause
unacceptable interferences to other electronic
equipments, this requiring the operator to take
all necessary steps to correct interferences.
Any variation introduced by the user to the
supplied equipment may degrade the
instruments' EMC performance.
To avoid damages or burns, do not put the
instrument in microwave ovens. For yours and
the instrument safety do not use or store the
instrument in hazardous environments.
WARRANTY
HI 83742 is warranted for two years against defects in workmanship and materials when used for
its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
2
Hanna Instruments reserves the right to modify the design, construction and appearance of its products
without advance notice.
27
ACCESSORIES
PRELIMINARY EXAMINATION
REAGENT SETS
HI 83742-20 Phenols reagent set for wine (20 tests)
HI 83742-25 Color reagent set for wine (20 tests) containing HI 83742-0 Wine Solvent 1
HI 83742-27 Color reagent set for wine (20 tests) containing HI 83742-3 Wine Solvent 3
OTHER ACCESSORIES
DEMI-10 Bottle to prepare 10 liters of demineralized water
HI 740027P 1.5V AA batteries (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731321 Glass cuvets (4 pcs)
HI 731325W Caps for cuvets (4 pcs)
HI 93703-50 Cuvets cleaning solution (230 mL)
HI 740226 5 mL graduated syringe
HI 731340 200 µL automatic pipette
HI 731350 Plastic tips for 200 µL automatic pipette (25 pcs)
HI 731342 2000 µL automatic pipette
HI 731352 Plastic tips for 2000 µL automatic pipette (25 pcs)
HI 740157 Plastic refilling pipette (20 pcs)
Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occured during shipment, please notify your Dealer.
Each HI 83742 Ion Selective Meter is supplied complete with:
• Two sample cuvets and caps
• Reagents for 5 tests (HI 83742-O, HI 83742A-O HI 83742B-O, HI 83742C-O)
• One 200 µL automatic pipette with two tips and Instruction Sheet
• One 2000 µL automatic pipette with two tips
• One 5 mL syringe with tip
• One 1 mL plastic pipette
• One 3 mL plastic pipette
• 12 VDC transformer (
• Four 1,5V AA batteries
• Tissue for wiping cuvets
• Instruction manual
• Instrument Quality Certificate
• Rigid carrying case
HI 710005 HI 710005
HI 710005 or
HI 710005 HI 710005
HI 710006HI 710006
HI 710006)
HI 710006HI 710006
BATTERY REPLACEMENT
Battery replacement must only take place in a nonhazardous area.
The blinking “
gets low.
When batteries are completely discharged, “0% bAtt”
will appear and after two seconds the instrument is
switched off.
Remove the battery cover from the bottom of the
instrument and change the old batteries with 4 fresh
1.5V batteries, paying attention to the correct polarity.
Replace the cover.
” will appear when the batteries power
26
Note: save all packing material until you are sure that the instrument works correctly. Any defective
item must be returned in its original packing.
3
GENERAL DESCRIPTION
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
The HI 83742 is an auto-diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has an advanced optical system
based on a special tungsten lamp and a narrow band interference filter that allows most accurate
and repeatable readings. All instruments are factory calibrated.
The auto-diagnostic feature of this meter ensures always optimal measurement conditions to
ensure most precise readings. The light level is automatically adjusted each time a zero-measurement
is made, and the temperature of the lamp is controlled to avoid overheating.
SIGNIFICANCE OF USE
Color determination of wine
Analytical techniques have become a valuable tool of modern wine makers. Especially the definition and
the processing techniques to obtain the desired wine color are of key importance. The right decisions taken
during maturation of the grapes, processing, aging and blending, all strongly influence the final result of
wine color.
The color of wine is always read after removal of suspended matter. There are manly two color components
present, yellow and red but also a blue or green hue may appear. The color hue is the ratio between the
yellow color concentrations over the red one, and is an indication about the degree of evolution.
The yellow color in wine comes from the present of tannins (polymers of flavonoid -procyanidins type, and
non-flavonoid phenols) and can be read without dilution. The increase of the yellow-brown color in older
wines is due to aging or oxidation.
The red colors of wines are caused by free anthocyanins, copigments of anthocyanins, and polymerized
phenolic compounds. The color of these pigments is pH dependent and can be intense dark. It is therefore
necessary to dilute the wine sample taking care not to change the original wine pH. Hanna recommends
using the special wine solvent to minimize possible errors due to dilution.
• Press “RANGE” to select the parameter code “P5” for
total phenols, red wine (see page 13).
• Fill an empty cuvet with deionized water to the 10 mL
mark and replace the cap. This is the
• Insert the zero cuvet into the holder and close the lid.
• Press ZERO and “----” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
• Insert the sample (cuvet #2) into the holder and
close the lid.
• Press READ/TIMER and “----” will blink during
measurement.
zero.zero.
zero.
zero.zero.
#1
(Zero)
#2
(Sample)
Phenol determination of wine
Phenolic compounds are important for several reasons since they (i) affect the color of the wine, (ii) have
an astringent taste, (iii) may case pungent odder, (iv) are a source of oxygen reduction, and (v) are sources
of browning substances.
Wine can contain a large variety of phenolic compounds and with traditional analytical techniques it is
difficult to distinguish between total phenols and specific phenols. Although some progress has been made
with HPLC, the most common analyses for total phenols remain the reaction of phenolic substances with
the Folin-Ciocalteu reagent. Other methods like the direct spectrophotometric determinations are less
accurate, because of difference in specific molar absorptivity, and color present of non phenolic substances.
4
• The instrument directly displays concentration in g/L
of GAE (gallic acid equivalent) on the Liquid Crystal
Display.
Note:
If the phenol concentration is higher than 5 g/L,
then pre-dilute the wine sample in the following way:
use the automatic pipette to add 2 mL of deionized
water and 2 mL of wine sample. Then follow the normal
sample preparation procedure, using this pre-diluted
sample as red wine sample. In this case the final value
must be multiplied by
2.
25
• Use the 200 µL automatic pipette with a clean
pipette tip to add exactly 0,2 mL of diluted red
wine sample.
• Add 6 drops of HI 83742B-0 Folin & Ciocalteu’s
Reagent to the cuvet.
• Replace the cap and shake gently to mix.
•
Wait for 1 minute and then use the 3 mL pipette to
add HI 83742C-0 Reagent C Carbonate Buffer, to
bring the
volume up to the 10 mL mark.
• Turn the meter ON and press RANGE to select the
parameter code “P5” for total phenols, red wine (see
page 13). Then hold down READ/TIMER for several
seconds. The instrument will show the countdown.
Alternatively, wait for 2 hours.
The instrument gives an acoustic signal to alert the
user that the countdown is finished.
• This is the reacted sample.
x 6
10 mL
#2
(Sample)
The reaction between phenols and the Folin-Ciocalteu reagent involves oxidation of the phenolic groups (ROH) with a mixture of phosphotungstenic acid (H3PW12O40) and phosphomolybdenic acid (H3PMo12O40) to
the quinoid form (R=O). The concomitant reduction of the Folin-Ciocalteu reagent causes a blue color in
the sample that is proportional to the total phenolic content that, in turn, is expressed as g/L of Gallic Acid
Equivalents (GAE).
Another common method to express the phenol content is to report the Folin Index (FI) or Total Phenol
Index. Simply multiply the read value in g/L of GAE times 25, to find the Folin Index value.
SPECIFICATIONS
Light Detector Silicon Photocell
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 4 x 1,5 volt AA batteries / 12 to 20 VDC through voltage adapter
Auto-Shut off
Dimensions 225 x 85 x 80 mm (8.7 x 3.3 x 3.1")
Weight 500 g (17,6 oz.).
After 15' of non-use.
PRECISION AND ACCURACY
Precision is how closely repeated measurements agree
with each other. Precision is usually expressed as
standard deviation (SD). Accuracy is defined as the
nearness of a test result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure explains
these definitions.
For example for phenols: in a laboratory using a
standard solution of 0.350 g/L gallic acid and a
representative lot of reagent, an operator obtained
with a single instrument a standard deviation of
0.015 g/L.
24
5
PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the
Lambert-Beer Law:
Where:
-log I/I
= Absorbance (A)
o
Io= intensity of incident light beam
I = intensity of light beam after absorption
ε
= molar extinction coefficient at wavelength λ
λ
c = molar concentration of the substance
d = optical path through the substance
physical-chemical characteristics of the substance according to the
-log I/Io = ε
λ
c d
or
A = ε
c d
λ
TOTAL PHENOLS, RED WINE
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
Range 0.00 to 5.00 g/L
Resolution 0.01
Precision ±0.10 @ 2.00 g/L
Light Source
Method The reaction between Phenols and the reagents causes a blue tint in the sample.
REQUIRED REAGENTSREQUIRED REAGENTS
REQUIRED REAGENTS
REQUIRED REAGENTSREQUIRED REAGENTS
Code Description Quantity/test
HI 83742A-0 Acid Reagent 5 mL
HI 83742B-0 Folin & Ciocalteu’s Reagent 6 drops
HI 83742C-0 Carbonate Buffer 4.5 mL
For reagent set and other accessories see page 26.
SAMPLE PREPARATIONSAMPLE PREPARATION
SAMPLE PREPARATION
SAMPLE PREPARATIONSAMPLE PREPARATION
• Use the 2000 µL automatic pipette to add exactly 2
mL of deionized water to an empty cuvet.
For a correct use of the automatic pipette please follow
the related Instruction Sheet.
Tungsten lamp with narrow band interference filter @ 610 nm
2 mL
200 µL
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from
a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 83000 series colorimeters is based on special subminiature
tungsten lamps and narrow-band interference filters to guarantee both high performance and
reliable results.
Block diagram (optical layout)
6
• Then use the 200 µL automatic pipette to add exactly
0,2 mL of red wine.
• Replace the cap and shake gently to mix. This is the
diluted red wine sample.
• Take another empty cuvet and use the 5 mL syringe
to add exactly 5 mL of HI 83742A-0 reagent to an
empty cuvet.
Note: in order to measure exactly 5 mL of reagent
with the syringe, follow the instructions on page 11.
23
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
• Press “RANGE” to select the parameter code “P4” for
total phenols, white wine (see page 13).
• Fill an empty cuvet with deionized water and replace
the cap. This is the
• Insert the zero cuvet into the holder and close the lid.
• Press ZERO and “----” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
zerozero
zero.
zerozero
#1
(Zero)
A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvet. The optical path is fixed by the diameter of the
cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of
intensity I
The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it
into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvet for both. It is necessary that the surface of the cuvet is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.
or I.
o
• Insert the sample (cuvet #2) into the holder and
close the lid.
• Press READ/TIMER and “----” will blink during
measurement.
• The instrument directly displays concentration in g/L
of GAE (gallic acid equivalent) on the Liquid Crystal
Display.
Note: If the phenols concentration is 0,750 g/L or
higher, please repeat the test following the instruction
for red wine (page 23).
22
#2
(Sample)
ABBREVIATIONS
degree Celsius
°C:
degree Fahrenheit
°F:
grams per liter. g/L is equivalent to ppt (part per thousand)
g/L:
milliliter
mL:
Liquid Crystal Display
LCD:
7
FUNCTIONAL DESCRIPTION
INSTRUMENT DESCRIPTION
FRONT
REAR
1) Lid
2) Cuvet Holder
3) Liquid Crystal Display (LCD)
4) ON/OFF key, to turn the meter on and off
5) ZERO key, to zero the meter
6) RANGE key, to select parameter
7) READ/TIMER key, to perform measurement
8) Power Socket 12V to 20V DC 2.5 Watt
• Add 6 drops of HI 83742B-0 Folin & Ciocalteu’s
Reagent to the cuvet.
• Replace the cap and shake gently to mix.
•
Wait for 1 minute and then use the 3 mL pipette to
add HI 83742C-0 Reagent C Carbonate Buffer, to
bring the
volume up to the 10 mL mark.
x 6
DISPLAY ELEMENTS DESCRIPTION
1) Four digit main display.
2) Battery icon: appears when the battery voltage is getting low.
3) The hourglass icon: appears during the countdown.
4) Status information.
5) Measurement unit.
6) Lamp status indicator.
7) Four digit secondary display.
8
• Turn the meter ON and press RANGE to select the
parameter code “P4” for total phenols, white wine
(see page 13). Then hold down READ/TIMER for
several seconds. The instrument will show the
countdown. Alternatively, wait for 2 hours.
The instrument gives an acoustic signal to alert the
user that the countdown is finished.
• This is the reacted sample.
21
10 mL
#2
(Sample)
TOTAL PHENOLS, WHITE WINE
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
Range 0.000 to 0.750 g/L
Resolution 0.001
Precision ±0.015 @ 0.350 g/L
Light Source
Method The reaction between Phenols and the reagents causes a blue tint in the
REQUIRED REAGENTSREQUIRED REAGENTS
REQUIRED REAGENTS
REQUIRED REAGENTSREQUIRED REAGENTS
Code Description Quantity/test
HI 83742A-0 Acid Reagent 5 mL
HI 83742B-0 Folin & Ciocalteu’s Reagent 6 drops
HI 83742C-0 Carbonate Buffer 4.5 mL
For reagent set and other accessories see page 26.
SAMPLE PREPARATIONSAMPLE PREPARATION
SAMPLE PREPARATION
SAMPLE PREPARATIONSAMPLE PREPARATION
• Use the 5 mL syringe to add exactly 5 mL of
HI 83742A-0 reagent to an empty cuvet.
Note: in order to measure exactly 5 mL of reagent
with the syringe, follow the instructions on page 11.
Tungsten lamp with narrow band interference filter @ 610 nm
sample.
GUIDE TO DISPLAY CODES
This prompt appears for a few seconds each time the instrument is
turned ON.
These prompts indicate the type of power supply: “Line” (if the
external power supply is used) or the battery level.
Indicates that the instrument is in a ready state and waiting for the
next command (Timer or Zero).
After Timer is pressed, a blinking hourglass icon appears and the
display shows a 2 hours coundown. Also the Zero tag might blink
if no zero measurement has been made before. At the end of the
countdown an acoustic signal alerts the user that the timer is
finished.
Indicates that the meter is performing a zero measurement. The
light intensity is automatically re-adjusted (auto-calibration features)
if necessary.
• Use the 200 µL automatic pipette to add exactly 0,2
mL of white wine sample to an empty cuvet.
For a correct use of the automatic pipette please
follow the related Instruction Sheet.
20
5 mL
The instrument is zeroed and a measurement can be made.
Indicates that the meter is making a measurement.
Batteries voltage is getting low and the batteries need to be
replaced.
9
Indicates that the batteries are dead and must be replaced. After this
message appears, the instrument is switched off. Change the batteries
and restart the meter.
ERROR MESSAGES
• Fill an empty cuvet with deionized water and replace
the cap. This is the
zerozero
zero.
zerozero
#1
(Zero)
The meter has lost its configuration. Contact your dealer or the nearest
Hanna Customer Service Center.
a) on zero reading:a) on zero reading:
a) on zero reading:
a) on zero reading:a) on zero reading:
“Light high”: there is too much light to perform a measurement.
Please check the preparation of the zero cuvet.
“Light low”: there is not enough light to perform a measurement.
Contact your dealer or the nearest Hanna Customer Service Center.
“No Light”: the instrument cannot adjust the light level. Contact your
dealer or the nearest Hanna Customer Service Center.
b) on sample reading:b) on sample reading:
b) on sample reading:
b) on sample reading:b) on sample reading:
• Insert the zero cuvet into the holder and close the lid.
• Press ZERO and “----” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
#2
(Sample)
• Insert the sample (cuvet #2) into the holder and
close the lid.
“Inverted”: the sample and the zero cuvet are inverted.
The sample absorbs less light than the zero reference. Check the procedure and make sure you use the same cuvet for reference (zero)
and measurement.
A flashing value of the maximum concentration indicates an over
range condition. The concentration of the sample is beyond the
programmed range: dilute the sample and measure again.
10
• Press READ/TIMER and “----” will blink during
measurement.
• The instrument directly displays the color hue on the
Liquid Crystal Display.
INTERFERENCES
Suspended matter should be removed by centrifugation or prior filtration. Use an adequate filter
material that does not absorb color.
19
COLOR HUE
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
Range 0.00 to 9.99
Resolution 0.01
Precision ±0.03 @ 0.75
Light Source
Method Direct reading.
REQUIRED REAGENTSREQUIRED REAGENTS
REQUIRED REAGENTS
REQUIRED REAGENTSREQUIRED REAGENTS
Code Description Quantity/test
HI 83742-0 Wine Solvent-1 8 mL
For reagent set and other accessories see page 26.
SAMPLE PREPARATIONSAMPLE PREPARATION
SAMPLE PREPARATION
SAMPLE PREPARATIONSAMPLE PREPARATION
•
Use the 2000 µL automatic pipette to add exactly
2
mL of wine sample to an empty cuvet. For a correct
use of the automatic pipette please follow the related
Instruction Sheet.
•
Then use the 1 mL plastic pipette to fill the cuvet
with HI 83742-0 Wine Solvent-1 up to the 10 mL
mark.
Tungsten lamp with narrow band interference filters @ 420 and 520 nm
10 mL
The instructions listed below should be carefully followed during testing to ensure best accuracy.
• For a correct filling of the cuvet: the liquid in the
cuvet forms a convexity on the top; the bottom of
this convexity must be at the same level of the
10 mL mark.
• For dosing the wine sample, we recommend to
use the supplied Hanna automatic pipettes
(HI 731340 - 200 µL, HI 731342 - 2000 µL).
For a correct use of the Hanna automatic pipette,
please follow the related Instruction Sheet.
• In order to avoid reagent leaking and to obtain
more accurate measurements, it is recommended
to close the cuvet first with the supplied HDPE
plastic stopper
cap.
• In order to measure exactly 5 mL of reagent with the 5 mL syringe:
(a) push the plunger completely into the syringe and insert the tip into the reagent bottle.
(b) pull the plunger up until the lower edge of the seal is exactly on the 5 mL mark.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are
hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in
vertical position above the cuvet, push the plunger completely down into the syringe. Now
the exact amount of 5 mL has been added to the cuvet.
and then with the black
10 mL
Hanna
automatic pipette
• Replace the cap. This is the sample.
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
• Press “RANGE” to select the parameter code “P3” for
color hue (see page 13).
18
#2
(Sample)
(a) (b) (c)
11
• Proper use of the dropper:
(a) to get good reproducible results, tap the dropper on the table for several times and wipe
the outside of the dropper tip with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
• Press “RANGE” to select the parameter code “P2” for
color density - red wine (see page 13).
(a) (b)
• Whenever the cuvet is placed into the measurement
cell, it must be dry outside, and completely free of
fingerprints, oil or dirt. Wipe it thoroughly with
HI 731318 (tissue for wiping cuvets, see chapter
ACCESSORIES) or a lint-free cloth prior to insertion.
• Do not let the reacted sample stand too long after
reaction, or accuracy will be lost.
• After the reading it is important to discard immediately
the sample, otherwise the glass might become
permanently stained.
• All the reaction times reported in this manual are
referred to 20°C (68°F). As a general rule of thumb,
they should be doubled at 10°C (50°F) and halved at
30°C (86°F).
• Fill an empty cuvet with deionized water and replace
the cap. This is the
• Insert the zero cuvet into the holder and close the lid.
• Press ZERO and “----” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
• Insert the sample (cuvet #2) into the holder and
close the lid.
zerozero
zero.
zerozero
#1
(Zero)
#2
(Sample)
12
• Press READ/TIMER and “----” will blink during
measurement.
• The instrument directly displays the color density on
the Liquid Crystal Display.
INTERFERENCES
Suspended matter should be removed by centrifugation or prior filtration. Use an adequate filter
material that does not absorb color.
17
COLOR DENSITY, RED WINE
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
Range 0.00 to 15.00
Resolution 0.01
Precision ±0.20 @ 5.00
Light Source
Method Direct reading.
REQUIRED REAGENTSREQUIRED REAGENTS
REQUIRED REAGENTS
REQUIRED REAGENTSREQUIRED REAGENTS
Code Description Quantity/test
HI 83742-0 Wine Solvent-1 8 mL
HI 83742-3 Wine Solvent-3 8 mL [optional reagent]
For reagent set and other accessories see page 26.
SAMPLE PREPARATIONSAMPLE PREPARATION
SAMPLE PREPARATION
SAMPLE PREPARATIONSAMPLE PREPARATION
•
Use the 2000 µL automatic pipette to add exactly
2
mL of red wine sample to an empty cuvet. For a
correct use of the automatic pipette please follow the
related Instruction Sheet.
•
Then use the 1 mL plastic pipette to fill the cuvet
with HI 83742-0 Wine Solvent-1 (or with HI 83742-3
Wine Solvent-3 - optional reagent) up to the 10 mL
mark.
Note: HI 83742-0 Wine Solvent 1 is used to measure
the true color of red wines and the results obtained
are conform to EC Directive 2676/90. The
corresponding replacement kit is HI 83742-25
(see page 26).
Note: HI 83742-3 Wine Solvent 3 is a stabilized and
ready-to-use solution at a fixed pH value (=3.2)
to compare different types of dark colored wine
samples often used for blending. The corresponding
replacement kit is HI 83742-27 (see page 26).
Tungsten lamp with narrow band interference filters @ 420 and 520 nm
or
( )
10 mL
optional
OPERATIONAL GUIDE
MEASUREMENT PROCEDURE
• Turn the instrument on by pressing ON/OFF.
• The meter will first perform an LCD self diagnostic test
by displaying a full set of figures.
• When the LCD displays “----”, it is ready. On the
secondary LCD a code indicates which parameter is
selected (“P1” or “P2” or... “P5”). The display code
that appears is the one of the last selected parameter.
• Press the RANGE key to select the desired parameter.
• After the desired parameter code appears on the display,
perform a measurement procedure following the
instructions reported in the related chapter.
PARAMETER REFERENCE TABLE
HI 83742 - COLOR & PHENOLS
Code Parameter Page
1 Color Density, white wine 14
2 Color Density, red wine 16
3 Color Hue 18
4 Total Phenols, white wine 20
5 Total Phenols, red wine 23
• Replace the cap. This is the sample.
16
#2
(Sample)
13
COLOR DENSITY, WHITE WINE
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
Range 0.000 to 1.000
Resolution 0.001
Precision ±0.010 @ 0.200
Light Source Tungsten lamp with narrow band interference filter @ 420 nm
Method Direct reading.
REQUIRED REAGENTSREQUIRED REAGENTS
REQUIRED REAGENTS
REQUIRED REAGENTSREQUIRED REAGENTS
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for measurement.
• Remove the cuvet from the instrument.
#2
(Sample)
No reagents required. For other accessories see page 26.
SAMPLE PREPARATIONSAMPLE PREPARATION
SAMPLE PREPARATION
SAMPLE PREPARATIONSAMPLE PREPARATION
• Use the plastic pipette to fill a cuvet with
and replace the cap. This is the sample.
MEASUREMENTMEASUREMENT
MEASUREMENT
MEASUREMENTMEASUREMENT
• Press “RANGE” to select the parameter code “P1” for
color density - white wine (see page 13).
• Fill an empty cuvet with deionized water and replace
the cap. This is the
• Insert the zero cuvet into the holder and close the lid.
zerozero
zero.
zerozero
white wine.
#2
(Sample)
#1
(Zero)
• Insert the sample (cuvet #2) into the holder and
close the lid.
• Press READ/TIMER and “----” will blink during
measurement.
• The instrument directly displays the color density on
the Liquid Crystal Display.
INTERFERENCES
Suspended matter should be removed by centrifugation or prior filtration. Use an adeguate filter
material that does not adsorb color.
• Press ZERO and “----” will blink on the display.
14
15
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