Hanna Instruments HI 83225 User Manual

Instruction Manual

HI 83225

Grow Master

for Nutrient Analyses

www.hannainst.com

MAN83225R1 04/05

This Instrument is in

Compliance with the CE Directiveswww.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. Please read this instruction

manual carefully before using the meter. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. These instruments are in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ....... 3

GENERAL DESCRIPTION ............... 3

SPECIFICATIONS ......................... 4

PRINCIPLE OF OPERATION .......... 4

FUNCTIONAL DESCRIPTION.......... 6

GUIDE TO DISPLAY CODES .......... 7

TIPS FOR AN ACCURATE

MEASUREMENT ........................ 10

PARAMETERS REFERENCE TABLE 11

OPERATIONAL GUIDE ................ 12

ABBREVIATIONS ....................... 13

INTRODUCTION ........................ 14

SAMPLE PREPARATION .............. 19

AMMONIA LR ........................... 23

AMMONIA MR .......................... 25

AMMONIA HR ........................... 27

CALCIUM .................................. 29

MAGNESIUM ............................ 31

NITRATE LR .............................. 33

NITRATE MR ............................. 35

NITRATE HR ............................. 37

PHOSPHORUS LR ..................... 39

PHOSPHORUS MR .................... 41

PHOSPHORUS HR ..................... 43

POTASSIUM LR ......................... 45

POTASSIUM MR........................ 47

POTASSIUM HR ........................ 49

POTASSIUM UHR ...................... 50

SULFATE .................................. 51

INTERFACE WITH PC ................. 53

STANDARD METHODS ............... 55

BATTERY REPLACEMENT ............ 55

ACCESSORIES ........................... 56

WARRANTY ............................... 57

CE DECLARATION

OF CONFORMITY....................... 58

HANNA LITERATURE .................. 59

HANNA LITERATURE

Hanna publishes a wide range of catalogs and handbooks for an equally wide range of applications. The reference literature currently covers areas such as:

• Water Treatment

• Process

• Swimming Pools

• Agriculture

• Food

• Laboratory

• Thermometry

and many others. New reference material is constantly being added to the library.

For these and other catalogs, handbooks and leaflets contact your dealer or the Hanna Customer Service Center nearest to you. To find the Hanna Office in your vicinity, check our home page at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

CE DECLARATION OF CONFORMITY

Recommendations for Users

Before using these products, make sure that they are entirely suitable for the environment in which they are used.

Operation of these instruments in residential area could cause unacceptable interferences to radio and TV equipments, requiring the operator to take all necessary steps to correct interferences.

Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance.

To avoid damages or burns, do not perform any measurement in microwave ovens.

PRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipment. If there is any damage, notify your Dealer.

Each HI 83225 Meter is supplied complete with:

• Four Sample Cuvets and Caps

• One Transport Cap

• Two 9 V Battery

• 12 VDC Transformer (HI 710005 or HI 710006)

Note: Save all packing material until you are sure that the instrument

functions correctly. Any defective item must be returned in its original packing with the supplied accessories.

GENERAL DESCRIPTION

HI 83000 Series is a line of 18 different bench, microprocessor-based photometers that measure more than 50 parameters in water and wastewater. These multipurpose meters are manufactured to measure the most important parameters of the application they have been especially designed for:

HI 83099 Laboratories, with COD HI 83200 Laboratories HI 83203 Aquaculture HI 83205 Boilers & Cooling Towers HI 83206 Environmental Testing HI 83207 Industrial Wastewater HI 83208 Water Conditioning HI 83209 Education HI 83210 Pulp & Paper Mills HI 83211 Chemical Manufacturers HI 83212 Power Plant Utilities HI 83213 Municipal Wastewater HI 83214 Wastewater Treatment Applic. HI 83215 Nutrient Analyses HI 83216 Pool & Spa Applic. HI 83218 Environmental Applic. HI 83225 Nutrient Analyses HI 83226 Pool & Spa Applic. All meters use an exclusive positive-locking system to ensure that the cuvet is in the same position every time it is placed into the measurement cell. The reagents are in liquid or powder form and are supplied in bottles or in packets. The amount of reagent is precisely dosed to ensure the maximum repeatability. Display codes aid the user in routine operations. The meters have an auto-shut off feature, turning the unit off after 10 minutes of non-use. The HI 83000 Series can be connected to a personal computer via the HI 920010 three wire RS 232 cable. The HI 92000 Hanna Windows Compatible Software aids the user to manage all test data.

SPECIFICATIONS

WARRANTY

Light Source Tungsten lamp with narrow band interference

filter

Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F);

max 95% RH non-condensing

Power Supply 2x 9 V batteries / 12 to 20 VDC through voltage

adapter

Auto-Shut off After 10' of non-use Dimensions 230 x 165 x 70 mm (9.0 x 6.5 x 2.8") Weight 640 g (22.6 oz.)

For specifications related to each single parameter (e.g. range, accuracy, etc.), refer to the related measurement section.

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between Electromagnetic Radiation and Matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physicochemical characteristics of substance according to the Lambert-Beer Law:

= ε

-log I/I A = ε

c d

All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for their intended purpose and maintained according to instructions.

This warranty is limited to repair or replacement free of charge. Damages due to accidents, misuse, tampering or lack of prescribed

maintenance are not covered. If service is required, contact the dealer from whom you purchased the

instrument. If under warranty, report the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.

Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice.

Where:

-log I/I

= Absorbance (A)

= intensity of incident light beam

I = intensity of light beam after absorption

= molar extinction coefficient at wavelength λ

c = molar concentration of the substance d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.

Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents.

ACCESSORIES

REAGENT SETS HI 93706-01 100 Phosphorus tests HI 93706-03 300 Phosphorus tests HI 93715-01 100 Ammonia tests HI 93715-03 300 Ammonia tests HI 93728-01 100 Nitrate tests HI 93728-03 300 Nitrate tests HI 93750-01 100 Potassium tests HI 93750-03 300 Potassium tests HI 93751-01 100 Sulfate tests HI 93751-03 300 Sulfate tests HI 937520-01 50 Magnesium tests HI 937520-03 150 Magnesium tests HI 937521-01 50 Calcium tests HI 937521-03 150 Calcium tests

OTHER ACCESSORIES HI 721310 9V battery (10 pcs) HI 731318 Tissue for wiping cuvets (4 pcs) HI 731321 Glass cuvets (4 pcs) HI 731325N New cap for cuvet (4 pcs) HI 740034 Cap for 100 mL beaker (6 pcs) HI 740036 100 mL plastic beaker (6 pcs) HI 740157 Plastic refilling pipette (20 pcs) HI 92000 Windows HI 920010 PC connection cable HI 93703-50 Cuvets cleaning solution (230 mL) HI 740223 170 mL plastic beaker HI 740224 170 mL plastic beakers (12 pcs) HI 740225 60 mL graduated syringe HI 740226 5 mL graduated syringe HI 740227 Filter assembly HI 740228 Filter discs (25 pcs) HI 740229 100 mL Polypropylene cylinder HI 740230 230 mL Demineralized water HI 93703-54 Dried resin (100 g) HI 93703-55 Activated Carbon (50 pcs)

Compatible Software

Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements.

The optical system of Hanna's HI 83000 multiparameter photometers is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results.

Four measuring channels (at four different wavelengths) allow a wide range of tests.

HI 83000 Block diagram (optical layout)

A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvet. The optical path is fixed by the diameter of the cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity I

or I.

The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range.

The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed.

The cuvet has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvets are optically identical to provide the same measurement conditions. Whenever possible use the same cuvet for both.

It is also necessary that the surface of the cuvet is clean and not scratched. This is to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvet walls with hands.

Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases, it is necessary to close the cuvet to prevent any contamination.

FUNCTIONAL DESCRIPTION

STANDARD METHODS

FRONT PANEL

1) Cuvet Holder

2) Dual Level Liquid Crystal Display

3) Programs List

4) READ DIRECT, to perform measurement immediately

5) TIMER, to perform measurements after a preprogrammed countdown

6) ZERO, to zero the meter prior to measurement

7) Program W and V, to select the desired parameter

8) ON/OFF, to turn the meter on and off

REAR PANEL

1) Power Socket 12V to 20V DC 2.5 Watt

2) RS 232 Socket

3) Batteries Compartment

Description Program Range Method

Ammonia, LR 1 0.0 to 10.0 mg/L Nessler Ammonia, MR 2 0.0 to 50.0 mg/L Nessler Ammonia, HR 3 0 to 100 mg/L Nessler Nitrate, LR 4 0.0 to 30.0 mg/L Cadmium Reduction Nitrate, MR 5 0 to 150 mg/L Cadmium Reduction Nitrate, HR 6 0 to 300 mg/L Cadmium Reduction Phosphorus, LR 7 0.0 to 10.0 mg/L Aminoacid Phosphorus, MR 8 0.0 to 50.0 mg/L Aminoacid Phosphorus, HR 9 0 to 100 mg/L Aminoacid Potassium, LR 10 0.0 to 20.0 mg/L Turbidimetric Potassium, MR 11 10 to 100 mg/ L Turbidimetric Potassium, HR 12 20 to 200 mg /L Turbidimetric Calcium, 13 0 to 400 mg/L Oxalate Magnesium, HR 14 0 to 150 mg/L Calmagite Sulfate 15 0 to 150 mg/L Turbidimetric

BATTERY REPLACEMENT

Besides to the 12 VDC supply, this meter can also be fed by two 9V batteries.

To prolong battery life, switch your meter off after use. However, the meter has an auto-shut off feature that will turn itself off after 10 minutes of non-use.

A blinking "LOBAT" on the display, while a measurement is in progress, indicates low voltage and the batteries should be replaced.

If the batteries are not replaced immediately, in order to prevent erroneous readings due to low voltage, "-BA-" is displayed soon afterwards. At this point the batteries must be changed.

Battery replacement must only take place in a non-hazardous area using two 9V alkaline batteries.

Remove the battery cover on the back of photometer, attach two fresh 9V batteries, while paying attention to the correct polarity, and replace the cover.

The meter will turn on automatically when a new battery is connected. You can turn it off by pressing ON/OFF.

/PUP - Program Up /PDN - Program Down /PTM - Turn Test Mode On /Brx - Set the baud rate

1 - 300 2 - 600 3 - 1200 4 - 2400

/KBL - Lock Keyboard /KBU - Unlock Keyboard ?PR# - Send Current Program Number (1 to 12) ?BRQ - Send current baud rate

1 - 150 2 - 300 3 - 600 4 - 1200 5 - 2400

?CNQ - Send Concentration (three bytes)

conc decimal point unit

m - ppm b - ppb t - ppt u - pcu h - pH

?ERR - send error / status information

0 - No error 1 - CAP 2 - HI 3 - ZERO 4 - LO 5 - IDLE 6 - ZERO DONE 7 - TIMED READ

GUIDE TO DISPLAY CODES

Note: The secondary LCD below shows a generic "P– –", whereas the

meter will indicate the exact program number (e.g. "P1" for Ammonia LR).

This indicates that the meter is in a ready state and zeroing can be performed.

Sampling in progress. This flashing prompt appears each time the meter is performing a measurement.

The microprocessor is adjusting the light level, indicated by a scrolling "SIP".

This indicates that the meter is in a zeroed state and measurement can be performed.

The instrument is performing an internal check-up.

Lotus 1-2-3

TELIX

Windows

and Windows Terminal® are registered Trademark of "Microsoft Co."

is registered Trademark of "Deltacomm"

The blinking "LOBAT" indicates that the battery voltage is getting low and the batteries need to be replaced.

This indicates that the batteries are dead and must be replaced.

ERROR MESSAGESERROR MESSAGES

ERROR MESSAGES

ERROR MESSAGESERROR MESSAGES a) on zero reading:a) on zero reading:

a) on zero reading:

a) on zero reading:a) on zero reading:

Light over range. The cuvet is not inserted correctly and an eccess ambient light is reaching the detector. If the cuvet is properly inserted, then contact your dealer or the nearest Hanna Customer Service Center.

The lamp is not working properly. Contact your dealer or the nearest Hanna Customer Service Center.

This indicates that the meter has lost its configuration. Contact your dealer or the nearest Hanna Customer Service Center.

This indicates that the zeroing procedure failed due to a low signal-to-noise ratio. In this case press ZERO again.

The instrument cannot adjust the light level. Please check that the sample does not contain any debris.

There is not enough light to perform a measurement. Please check the preparation of the zero cuvet.

There is too much light to perform a measurement. Please check the preparation of the zero cuvet.

INTERFACE WITH PC

To connect your meter to the PC use the optional HI 920010 (available from your Hanna Dealer). Make sure that your meter is switched off and plug the connectors, one into the meter RS 232C socket, the other into the serial port of your PC.

Note: Cables other than HI 920010 may use a different configuration, in which case, communication between the meter and the PC may not be possible.

SETTING THE BAUD RATE

The transmission speed (baud rate) of the meter and the external device must be identical. The meter is factory set to 2400.

If you wish to change this value, please contact your nearest Hanna Center.

SENDING COMMANDS FROM PC

With terminal programs such as, for example, Telix®, Windows Termi-

nal

, it is possible to remotely control your Hanna Instruments bench

meter. Use HI 920010 cable to connect the meter to the PC, start the terminal program and set the communication options as follows: 8, N, 1, no flow control.

Command Types

To send a command to the pH meter the scheme is:

This line makes the computer send a Data Link Escape character (/ or ?), the command expressed as a number or a 3-character sequence and a CR character.

Note: Windows Terminal® and all the other terminal programs that support the ANSI escape sequence, represent the DLE character by the string ‘^P’ and the CR character by the string ‘^M’.

Type of Commands

/OFF - Turn the meter OFF /PDR - Press Read Direct /PTR - Press Timer /PZR - Press Zero

• Wait for a few seconds and the display will show "-0.0-". Now the meter is zeroed and ready for measurement.

• Remove the cuvet.

• Add the content of one packet of 93751 reagent

• Replace the cap and shake gently for 1 minute.

• Reinsert the cuvet into the instrument.

b) on sample reading:b) on sample reading:

b) on sample reading:

b) on sample reading:b) on sample reading:

There is too much light for the sample measurement. Please check if the right sample cuvet is inserted.

The sample and zero cuvet are inverted.

A zero reading was not taken. Follow the instruction described in the measurement

1’

procedures for zeroing the meter.

Under range. A blinking "0.00" indicates that the sample absorbs less light than the zero reference. Check the procedure and make sure that you use the same cuvet for reference (zero) and measurement.

• Press TIMER and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press READ DIRECT. In both cases “SIP” will blink during measurement.

• The instrument directly displays concentration in mg/L of Sulfate

(SO

) on the Liquid Crystal Display.

INTERFERENCES

Color or suspended matter in large amounts will interfere: suspended matter should be removed by previous filtration.

Organic matter in large amounts may impede the precipitation of barium sulfate.

Calcium (as CaCO Chloride (as Cl Magnesium (as MgCO Silica (as SiO

Sulfate

) >20000 mg/L

) >40000 mg/L

) >10000 mg/L

) >500 mg/L

1) A flashing value of the maximum concentration indicates an over range condition. The concentration of the sample is beyond the programmed range: dilute the sample and rerun the test.

2) A flashing value lower than the maximum concentration indicates a low signalto-noise ratio condition. In this case accuracy of the result is not guaranteed. Repeat the reading procedure.

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