Hanna instruments HI 83099 User Manual

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Instruction Manual

HI 83099

COD and Multiparameter Bench

Photometer

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the

instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ............................................. 3

GENERAL DESCRIPTION ..................................................... 3

ABBREVIATIONS ............................................................... 4

SPECIFICATIONS .............................................................. 4

PRECISION AND ACCURACY ............................................... 4

PRINCIPLE OF OPERATION ................................................ 5

FUNCTIONAL DESCRIPTION ............................................... 6

TIPS FOR AN ACCURATE MEASUREMENT ........................... 8

HEALTH & SAFETY ..........................................................11

METHOD REFERENCE TABLE ............................................. 11

OPERATIONAL GUIDE ....................................................... 12

SETUP ........................................................................... 14

HELP MODE ................................................................... 16

SAMPLE PREPARATION .................................................... 17

ALUMINUM ....................................................................21

ALKALINITY .................................................................... 23

AMMONIA MR ................................................................ 25

AMMONIA LR .................................................................27

BROMINE ......................................................................29

CALCIUM ....................................................................... 31

FREE CHLORINE ............................................................. 33

TOTAL CHLORINE ............................................................36

CHLORINE DIOXIDE ......................................................... 39

CHROMIUM VI HR ..........................................................42

CHROMIUM VI LR ...........................................................44

COLOR OF WATER ........................................................... 46

COPPER HR ....................................................................48

COPPER LR .................................................................... 50

CYANURIC ACID .............................................................. 52

FLUORIDE ...................................................................... 54

CALCIUM HARDNESS ....................................................... 56

MAGNESIUM HARDNESS ................................................. 59

HYDRAZINE .................................................................... 62

IODINE .......................................................................... 64

IRON HR ........................................................................ 66

IRON LR .........................................................................68

MAGNESIUM ..................................................................71

MANGANESE HR ............................................................. 73

MANGANESE LR ............................................................. 75

MOLYBDENUM ............................................................... 78

NICKEL HR ..................................................................... 81

NICKEL LR ...................................................................... 83

NITRATE .........................................................................86

NITRITE HR .................................................................... 88

NITRITE LR .....................................................................90

DISSOLVED OXYGEN ........................................................92

OXYGEN DEMAND, CHEMICAL HR ..................................... 94

OXYGEN DEMAND, CHEMICAL MR .....................................97

OXYGEN DEMAND, CHEMICAL LR .................................... 100

OZONE ........................................................................ 103

pH .............................................................................. 106

PHOSPHATE HR ............................................................ 108

PHOSPHATE LR .............................................................110

PHOSPHORUS ............................................................. 112

POTASSIUM HR ............................................................ 114

POTASSIUM MR ............................................................ 116

POTASSIUM LR ............................................................. 118

SILICA .........................................................................120

SILVER ........................................................................122

SULFATE ...................................................................... 125

ZINC ............................................................................127

ERRORS AND WARNINGS ............................................... 129

DATA MANAGEMENT ...................................................... 130

STANDARD METHODS .................................................... 131

ACCESSORIES .............................................................. 132

WARRANTY .................................................................. 135

HANNA LITERATURE ...................................................... 135

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your local Hanna Office.

Each meter is supplied complete with:

• Four Sample Cuvettes and Caps

• Sample Preparation Kit (for turbid or concentrated samples see page 17)

• Cloth for wiping cuvettes (1 pcs)

• 60 mL glass bottle for dissolved oxygen analysis (1 pcs)

• Scissors

• AC/DC Power Adapter

• Instruction Manual

The sample preparation kit contains:

• 4 cuvettes (10 mL) with caps

• 2 plastic beakers (100 and 170 mL)

• 1 graduated cylinder (100 mL)

• 1 syringe with screw rim (60 mL)

• 1 syringe (5 mL)

• 1 funnel

• 25 filter discs

• 1 spoon

• 2 pipettes

• Carbon powder packets (50 pcs)

• 1 Demineralizer Bottle with filter cap for about 12 liters of deionized water (depending on the hardness level of water to be treated)

Note: Save all packing material until you are sure that the instrument works correctly. Any defective item

must be returned in its original packing with the supplied accessories.

GENERAL DESCRIPTION

HI 83099 is a multiparameter bench photometer dedicated for Laboratory analysis. It measures 47 different methods using specific liquid or powder reagents. The amount of reagent is precisely dosed to ensure maximum reproducibility.

HI 83099 bench photometer can be connected to a PC via an USB cable. The optional HI 92000 Windows® Compatible Software helps users manage all their results.

HI 83099 has a powerful interactive user support that assists the user during the analysis process. Each step in the measurement process is help supported. A tutorial mode is available in the Setup Menu.

ABBREVIATIONS

EPA: US Environmental Protection Agency

°C: degree Celsius °F: degree Fahrenheit

μg/L: micrograms per liter (ppb)

mg/L: milligrams per liter (ppm)

g/L: grams per liter (ppt)

mL: milliliter

HR: high range

MR: medium range

LR: low range

PAN: 1-(2-pyridylazo)-2-naphtol

TPTZ: 2,4,6-tri-(2-pyridyl)-1,3,5-triazine

SPECIFICATIONS

Light Life Life of the instrument Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F);

max 90% RH non-condensing

Power Supply external 12 Vdc power adapter

built-in rechargeable battery

Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg

For specifications related to each method (e.g. range, resolution, etc.) refer to the related measurement section.

PRECISION AND ACCURACY

Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD).

Accuracy is defined as the nearness of a test result to the true value.

Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions.

For each method, the accuracy is expressed in the related measurement section.

PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of substance according to the Lambert-Beer Law:

-log I/Io = ε A = ε

Where:

-log I/I

= Absorbance (A)

Io= intensity of incident light beam I = intensity of light beam after absorption

= molar extinction coefficient at wavelength λ

c = molar concentration of the substance d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.

Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents.

Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements.

The optical system of HI 83099 is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results.

Five measuring channels allow a wide range of tests.

c d

Instrument block diagram (optical layout)

A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed through the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity Io or I.

The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range.

The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed.

The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvette are optically identical to provide the same measurement conditions. Most methods use the same cuvette for both, so it is important that measurements are taken at the same optical point. The instrument and the cuvette cap have special marks that must be aligned in order to obtain better reproducibility.

The surface of the cuvette must be clean and not scratched. This is to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvette walls with hands.

Furthermore, in order to maintain the same conditions during the zeroing and the measurement phases, it is necessary to cap the cuvette to prevent any contamination.

FUNCTIONAL DESCRIPTION

INSTRUMENT DESCRIPTION

1) Open Cuvette Lid

2) Indexing mark

3) Cuvette point

4) Liquid Crystal Display (LCD)

5) Splash proof keypad

6) ON/OFF power switch

7) Power input connector

8) USB connector

KEYPAD DESCRIPTION

The keypad contains 8 direct keys and 3 functional keys with the following functions:

Press to perform the function displayed above it on the LCD.

ESC

Press to exit the current screen.

Press to access the select method menu.

Press to move up in a menu or a help screen, to increment a set value, to access second level

functions.

RCL

HELP

SETUP

Press to move down in a menu or a help screen, to decrement a set value, to access second

level functions.

Press to log the current reading.

Press to recall the log.

Press to display the help screen.

Press to access the setup screen.

TIPS FOR AN ACCURATE MEASUREMENT

The instructions listed below should be carefully followed during testing to ensure most accurate results.

• Color or suspended matter in large amounts may cause interference, and should be removed by treatment with active carbon and filtration: refere to Sample Preparation Chapter (page 17).

• Ensure the cuvette is filled correctly: the liquid in the cuvette forms a convexity on the top; the bottom of this convexity must be at the same level as the 10 mL mark.

COLLECTING AND MEASURING SAMPLES

• In order to measure exactly 0.5 mL of reagent with the 1 mL syringe: (a) push the plunger completely into the syringe and insert the tip into the solution. (b) pull the plunger up until the lower edge of the seal is exactly on the 0.0 mL mark. (c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging

on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark. Now the exact amount of 0.5 mL has been added to the cuvette, even if the tip still contains some solution.

(c)(a) (b)

USING LIQUID AND POWDER REAGENTS

• Proper use of the dropper: (a) for reproducible results, tap the dropper on the table for several times and wipe the outside of the

dropper tip with a cloth.

(b) always keep the dropper bottle in a vertical position while dosing the reagent.

(a) (b)

• Proper use of the powder reagent packet: (a) use scissors to open the powder packet; (b) push the edges of the packet to form a spout; (c) pour out the content of the packet.

USING CUVETTES

• Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter.

(a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position. Turn

the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this mixing technique is 10-15 complete inversions in 30 seconds.

This mixing technique is indicated with “invert to mix” and the following icon:

(b) shaking the cuvette, moving the cuvette up and down. The movement may be gentle or vigorous.

This mixing method is indicated with “shake gently” or “shake vigorously”, and one of the following icons:

shake gently shake vigorously

• Pay attention to push the cuvette completely down in the holder and to align the white point on the cap to the indexing mark on the meter.

• In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic

stopper and then the black cap.

• Each time the cuvette is used, the cap must be tightened to the same degree.

• Whenever the cuvette is placed into the measurement cell, it must be dry outside, and free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion.

• Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.

• Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the timings described in each specific method.

• It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible (for most precise results follow the measurement procedures carefully).

• Discard the sample immediately after the reading is taken, or the glass might become permanently stained.

• All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F), and decreased for temperatures higher than 25 °C (77 °F).

INTERFERENCES

• In the method measurement section the most common interferences that may be present in an average sample matrix have been reported. It may be that for a particular treatment process other compounds do interfere with the method of analysis.

HEALTH & SAFETY

• The chemicals contained in the reagent kits may be hazardous if improperly handled.

• Read the Material Safety Data Sheet (MSDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.

• Waste disposal: for proper disposal of reagent kits and reacted samples, refer to the Material Safety Data Sheet (MSDS).

METHOD REFERENCE TABLE

Method Method Page

description

1 Aluminum 21 2 Alkalinity 23 3 Ammonia MR 25 4 Ammonia LR 27

5 Bromine 29

6 Calcium 31 7 Free Chlorine 33 8 Total Chlorine 36

9 Chlorine Dioxide 39 10 Chromium VI HR 42 11 Chromium VI LR 44 12 Color of Water 46 13 Copper HR 48 14 Copper LR 50 15 Cyanuric Acid 52 16 Fluoride 54 17 Calcium Hardness 56 18 Magnesium Hardness 59 19 Hydrazine 62 20 Iodine 64 21 Iron HR 66 22 Iron LR 68 23 Magnesium 71 24 Manganese HR 73

Method Method Page

description

25 Manganese LR 75 26 Molybdenum 78 27 Nickel HR 81 28 Nickel LR 83 29 Nitrate 86 30 Nitrite HR 88 31 Nitrite LR 90 32 Dissolved Oxygen 92 33 COD HR 94 34 COD MR 97 35 COD LR 100 36 Ozone 103 37 pH 106 38 Phosphate HR 108 39 Phosphate LR 110 40 Phosphorus 112 41 Potassium HR 114 42 Potassium MR 116 43 Potassium LR 118 44 Silica 120 45 Silver 122 46 Sulfate 125 47 Zinc 127

OPERATIONAL GUIDE

POWER CONNECTION AND BATTERY MANAGEMENT

The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery. Note: Always turn the meter off before unplugging it to ensure no data is lost. When the meter switches ON, it verifies if the power supply adapter is connected. The battery icon on the

LCD will indicate the battery status:

- battery is charging from external adapter - battery fully charged (meter connected to AC/DC adapter)

- battery capacity (no external adapter) - battery Low (no external adapter)

- battery Dead (no external adapter)

METHOD SELECTION

• Turn the instrument ON via the ON/OFF power switch.

• The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear

on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display.

• In order to select the desired method press the METHOD key and a screen with the available methods

will appear.

• Press the   keys to highlight the desired method. Press Select.

• After the desired method is selected, follow the measurement described in the related section.

• Before performing a test you should read all the instructions carefully.

DATA MANAGEMENT

The instrument features a data log function to help you keep track of all your analysis. The data log can hold 200 individual measurements. Storing, viewing and deleting the data is possible using the LOG and

RCL keys

Storing data

stored with date and time stamps.

: You can store only a valid measurement. Press LOG

and the last valid measurement will be

Viewing and deleting

the last saved measurement. Additionally, you can delete the data records all at once.

: You can view and delete the data log by pressing the RCL key. You can only delete

CHEMICAL FORM

Chemical form conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical form press  or  to access the second level function and then press the Chem Frm key to toggle between the available chemical forms for the selected method.

SPECIAL CONVERSIONS

For Magnesium and Calcium Hardness, special conversion factors can be used to convert the readings from mg/L to French degrees (°f), German degrees (°dH) and English degrees (°E) of hardness. This can be achieved by pressing  or  to access the second level functions and then press the Unit key to toggle between °f, °dH, °E and mg/L.

SETUPSETUP

SETUP

SETUPSETUP

In the Setup mode the instrument’s parameters can be changed. Some parameters affect the measuring sequence and others are general parameters that change the behavior or appearance of the instrument.

Press SETUP to enter the setup mode. Press ESC or SETUP to return to the main screen. A list of setup parameters will be displayed with currently configured settings. Press HELP for additional information. Press the   keys to select a parameter and change the

value as follows:

Backlight

Values: 0 to 8.

Press the Modify key to access the backlight value. Use the W X functional keys or the   keys to increase or decrease the value. Press the Accept functional key to confirm or ESC to return to the

setup menu without saving the new value.

Contrast

Values: 0 to 20.

This option is used to set the display’s contrast. Press the Modify key to change the display’s contrast. Use the W X functional keys or the   keys to increase or decrease the value. Press the Accept key to confirm the value or ESC to return to the

setup menu without saving the new value.

Date / Time

This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the W X functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the   keys to change the value. Press the Accept key to confirm or ESC to return to the setup

without saving the new date or time.

Time format

Option: AM/PM or 24 hour.

Press the functional key to select the desired time format.

Date format

Press the Modify key to change the Date Format. Use the   keys to select the desired format. Press Accept functional key to confirm or ESC to return to the

setup menu without saving the new format.

Language

Press the corresponding key to change the language. If the new language cannot be loaded, the previously selected language will be reloaded.

Tutorial

Option: Enable or Disable.

If enabled this option will provide the user short guide related to the current screen. Press the functional key to enable/disable the tutorial mode.

Beeper

Option: Enable or Disable.

When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected.

Press the functional key to enable/disable the beeper.

Instrument ID

Option: 0 to 9999.

This option is used to set the instrument’s ID (identification number). The instrument ID is used while exchanging data with a PC. Press the Modify key to access the instrument ID screen. Press the   keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the setup menu without saving the new value.

Meter information

Press the Select key to view the instrument model, firmware version, language version and instrument serial number.

Press ESC to return to the Setup mode.

HELP MODEHELP MODE

HELP MODE

HELP MODEHELP MODE

HELP MODE

HI 83099 offers an interactive contextual help mode that assists the user at any time.

To access the help screens press HELP. The instrument will display additional information related to the current screen. To read all the available information, scroll the text using the   keys. Press the Support key to access a screen with Hanna service centers and their contact details. Press the Accessories key to access a list of instrument reagents and accessories. To exit support or accessories screens press ESC and the instrument will return to the previous help screen. To exit help mode press the HELP or ESC key again and the meter will return to the previously selected screen.

SAMPLE PREPARATION

SAMPLE PREPARATION PROCEDURE

The following Sample Preparation Procedure applies in case of:

• Samples with color or suspended matter (turbidity).

• Concentrated samples, for which the analysis result is over the range of the parameter. Use the accessories contained in the Sample Preparation Kit to prepare the sample according to the

following instructions.

COLORED OR TURBID SAMPLES:

Colored or suspended matter in large amounts may cause interference. They should be removed by treatment with active carbon and filtration.

• If the water sample contains suspended matter, let it stand in a beaker until most of the solid particles have settled. Then, use the pipette to transfer the supernatant solution to the other beaker. To prevent the displacement of the settled solids at the bottom of the beaker, do not induce air bubbles into the solution.

• Measure 100 mL of sample with the graduated cylinder.

• If the solution still contains some turbidity or color, pour it in the large 170 mL beaker and add a powder packet of active carbon.

• Mix well using the spoon and then wait for 5 minutes.

• Fold a filter disc twice as shown in the figure. Separate one side from the other three to form a cone. Insert the folded filter disc in the funnel.

• Filter the treated sample into an empty beaker. The sample is now ready.

• If the solution is still turbid or colored, treat it again with a packet of active carbon. After use, throw the filter disc away and wash the syringe and the filter assembly well. Always use a new disc for another sample.

CONCENTRATED SAMPLES:

If the analysis result is over the method range, the sample should be diluted. The following procedure describe how to dilute the sample by a factor “N” (that is, to dilute by “N times”):

• Use the graduated cylinder to measure exactly V mL of sample. For volumes V < 20 mL, accurately dose the sample by mean of the syringe.

Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe.

probable level of liquid

taken up by syringe

• Remove the cap and fill the Demineralizer Bottle with tap water.

• Replace the cap and shake gently for at least 2 minutes.

• Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark.

Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance. The indicator will change from green to blue when the resin has been exhausted and needs to be replaced.

• Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix.

• If the solution contains some turbidity or color, add a powder packet of active carbon and follow the

procedure described in previous section Colored or Turbid Samples.

• Calculate the dilution factor N: N = 100/V Where: V is the volume of original sample poured in the cylinder, expressed in mL, and 100 is the final volume

in the cylinder, expressed in mL.

• When performing the reading, pay attention to multiply the read value by the dilution factor in order to obtain the real concentration of the analyte in the original sample:

Example: Reading = value A Dilution factor = N Real value in the original sample = A x N

Note: The methods Potassium Medium Range and Potassium High Range require a dilution of 1:5

(N = 5, V = 20 mL) and 1:10 (N = 10, V = 10 mL) of the sample. As the dilution is always done, it is already included in the final result and is not necessary to multiply by the dilution factor.

ALUMINUM

SPECIFICATIONS

Range 0.00 to 1.00 mg/L Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the aluminon method. The reaction between aluminum and reagents

causes a reddish tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93712A-0 Ascorbic acid 1 packet HI 93712B-0 Aluminon reagent 1 packet HI 93712C-0 Bleaching powder 1 packet

REAGENT SETS

HI 93712-01 Reagents for 100 tests HI 93712-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

Aluminum

Method Selection

method using the procedure described in section (see page 12).

• Fill a graduated beaker with 50 mL of sample.

• Add the content of one packet of HI 93712A-0 Ascorbic acid and mix until completely dissolved.

• Add the content of one packet of HI 93712B-0 Aluminon reagent and mix until completely dissolved. This is the sample.

• Fill two cuvettes with 10 mL of sample each (up to the mark).

10 mL

# 1

10 mL

# 2

Aluminum

• Add the content of one packet of HI 93712C-0 Bleaching powder to one of the two cuvettes. Replace the cap and shake vigorously until completely dissolved. This is the blank.

• Place the blank into the holder and close the lid.

# 1

• Press Timer and the display will show the countdown prior to

# 1

zeroing the blank. Alternatively wait for 15 minutes and then press Zero. The display will show “-0.0-“ when the meter is zeroed and ready for measurement.

# 2

• Remove the blank and insert the other cuvette into the instrument.

• Press the Read key and the meter will perform the reading. The instrument displays the results in mg/L of aluminum.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of Al2O3.

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Iron above 20 mg/L, Alkalinity above 1000 mg/L, Phosphate above 50 mg/L; Fluoride must be absent.

Aluminum

ALKALINITY

SPECIFICATIONS

Range 0 to 500 mg/L (as CaCO3) Resolution 5 mg/L Accuracy ±5 mg/L ±10 % of reading at 25 °C Typical EMC ±5 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Colorimetric Method. The reaction causes a distinctive range of colors from yellow to

green to greenish blue to develop.

REQUIRED REAGENTS

Code Description Quantity/test

HI 93755-0 Alkalinity Indicator Reagent 1 packet

REAGENT SETS

HI 93755-01 Reagents for 100 tests HI 93755-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Alkalinity

Method Selection

method using the procedure described in

section (see page 12).

10 mL

• Place the cuvette into the holder and close the lid

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Alkalinity

• Remove the cuvette.

• Carefully add the content of one packet of HI 93755-0 Alkalinity Indicator Reagent. Replace the cap and shake vigorously for 30 seconds. Note: Pay attention not to spill reagent otherwise full color development may be inhibited.

• Press Timer or wait for 2 minutes. Then invert 3 times the cuvette gently again.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading.

• The instrument displays the results in mg/L of alkalinity (CaCO3).

Alkalinity

AMMONIA MEDIUM RANGE

SPECIFICATIONS

Range 0.00 to 10.00 mg/L Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the

Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93715A-0 First Reagent 4 drops (6 drops for seawater) HI 93715B-0 Second Reagent 4 drops (10 drops for seawater)

REAGENT SETS

HI 93715-01 Reagents for 100 tests HI 93715-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Ammonia MR

Method Selection

section (see page 12).

ASTM Manual of Water and Environmental Technology, D1426-92

method using the procedure described in

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Ammonia MR

• Remove the cuvette.

• Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution.

• Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium (NH

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: acetone, alcohols, aldehydes, glycine, hardness above 1 g/L, iron, organic chloramines, sulfide, various aliphatic and aromatic amines.

Ammonia MR

AMMONIA LOW RANGE

SPECIFICATIONS

Range 0.00 to 3.00 mg/L Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the

Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93700A-0 First Reagent 4 drops (6 drops for seawater) HI 93700B-0 Second Reagent 4 drops (10 drops for seawater)

REAGENT SETS

HI 93700-01 Reagents for 100 tests HI 93700-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

Ammonia LR

Method Selection

and replace the cap.

section (see page 12).

method using the procedure described in the

ASTM Manual of Water and Environmental Technology, D1426-92

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

Ammonia LR

• Remove the cuvette.

• Add 4 drops of HI 93700A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution.

• Add 4 drops of HI 93700B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium (NH

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: acetone, alcohols, aldehydes, glycine, hardness above 1 g/L, iron, organic chloramines, sulfide, various aliphatic and aromatic amines.

Ammonia LR

BROMINE

SPECIFICATIONS

Range 0.00 to 8.00 mg/L Resolution 0.01 mg/L Accuracy ±0.08 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

18th edition

pink tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93716-0 DPD Reagent 1 packet

REAGENT SETS

HI 93716-01 Reagents for 100 tests HI 93716-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the

Bromine

method using the procedure described in the

Method Selection

mark) and replace the cap.

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

, DPD method. The reaction between bromine and the reagent causes a

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

Bromine

• Remove the cuvette and add the content of one packet of HI 93716-0 DPD reagent. Replace the cap and shake gently for about 20 seconds to dissolve most of the reagent.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading.

• The instrument displays the results in mg/L of bromine.

INTERFERENCES

Interference may be caused by: Chlorine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately

2 minutes after adding the reagent. In case of water with alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3,

the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Bromine

CALCIUM

SPECIFICATIONS

Range 0 to 400 mg/L Resolution 10 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source Tungsten lamp with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate method.

REQUIRED REAGENTS

Code Description Quantity

- Buffer Reagent 4 drops

HI 93752A-0 Ca Calcium Buffer Reagent 7 mL HI 93752B-0 Ca Calcium Oxalate Reagent 1 mL

REAGENT SETS

HI 937521-01 Reagents for 50 tests HI 937521-03 Reagents for 150 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

Note: for sample preparation follow the COLORED OR TURBID SAMPLES procedure at page 17.

• Select the

• Using the 5 mL syringe add exactly 3.00 mL of sample to the cuvette.

Calcium

method using the procedure described in the

Method Selection

section (see page 12).

3 mL of sample

• Use the pipette to fill the cuvette up to the 10 mL mark with the HI 93752A-0 Calcium Buffer Reagent.

• Add 4 drops of Buffer Reagent.

Calcium

• Replace the cap and invert several times to mix.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Using the 1 mL syringe, add exactly 1 mL of the HI 93752B-0 Calcium Oxalate Reagent. Replace the cap and invert the cuvette 10 times to mix (about 15 seconds).

• Press Timer or wait for 5 minutes. Then invert again the cuvette 10 times to mix (about 15 seconds).

• Reinsert the cuvette into the instrument.

• Press Read to start the reading. The instrument displays the results in mg/L of Calcium.

INTERFERENCES:

Interferences may be caused by: Acidity (as CaCO3) above 1000 mg/L Alkalinity (as CaCO3) above 1000 mg/L Magnesium (Mg2+) above 400 mg/L

Calcium

FREE CHLORINE

FREE CHLORINEFREE CHLORINE

FREE CHLORINE

FREE CHLORINEFREE CHLORINE

SPECIFICATIONS

Range 0.00 to 2.50 mg/L Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

DPD reagent causes a pink tint in the sample.

EPA DPD method 330.5.

The reaction between free chlorine and the

REQUIRED REAGENTS

POWDER: Code Description Quantity

HI 93701-0 DPD 1 packet

LIQUID: Code Description Quantity

HI 93701A-F DPD1 Indicator 3 drops HI 93701B-F DPD1 Buffer 3 drops

REAGENT SETS

HI 93701-F Reagents for 300 tests (liquid) HI 93701-01 Reagents for 100 tests (powder) HI 93701-03 Reagents for 300 tests (powder)

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the

• Place the cuvette into the holder and close the lid.

Free Chlorine

in the

Method Selection

mark) and replace the cap.

method using the procedure described

section (see page 12).

10 mL

Free Chlorine

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

Powder reagents procedure

• Add the content of one packet of HI 93701 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis).

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of free chlorine.

Liquid reagents procedure

• To an empty cuvette add 3 drops of HI 93701A-F DPD1 indicator and 3 drops of HI 93701B-F DPD1 buffer. Swirl gently to mix, and immediately add 10 mL of unreacted sample. Replace the cap and shake gently again.

Free Chlorine

• Place the cuvette into the instrument.

• Press Read to start the reading. The instrument displays the results in mg/L of free chlorine.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately

2 minutes after adding the powder reagent. In case of water with alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3,

the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Free Chlorine

TOTAL CHLORINE

SPECIFICATIONS

Range 0.00 to 3.50 mg/L Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

DPD reagent causes a pink tint in the sample.

REQUIRED REAGENTS

POWDER: Code Description Quantity

HI 93711-0 DPD 1 packet

LIQUID: Code Description Quantity

HI 93701A-T DPD1 indicator 3 drops HI 93701B-T DPD1 buffer 3 drops HI 93701C DPD3 solution 1 drop

REAGENT SETS

HI 93701-T Reagents for 300 total chlorine tests (liquid) HI 93711-01 Reagents for 100 total chlorine tests (powder) HI 93711-03 Reagents for 300 total chlorine tests (powder)

For other accessories see page 132.

EPA DPD method 330.5.

The reaction between the chlorine and the

MEASUREMENT PROCEDURE

• Select the described in the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

Total Chlorine

Method Selection

method using the procedure

section (see page 12).

10 mL

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

Powder reagents procedure

• Add 1 packet of HI 93711 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis).

• Reinsert the cuvette into the instrument.

chlorine.

Liquid reagents procedure

• To an empty cuvette add 3 drops of HI 93701A-T DPD1 indicator, 3 drops of HI 93701B-T DPD1 buffer and 1 drop of HI 93701C DPD3 solution. Swirl gently to mix and immediately add 10 mL of unreacted sample. Replace the cap and shake gently again.

• Reinsert the cuvette into the instrument.

Total Chlorine

• The instrument displays the results in mg/L of total chlorine.

Note: free and total chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are requested.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately

2 minutes after adding the powder reagent. In case of water with alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3,

the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Total Chlorine

CHLORINE DIOXIDE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the Chlorophenol Red method. The reaction between chlorine dioxide and

reagents causes a colorless to purple tint in the sample.

REQUIRED REAGENT

Code Description Quantity

HI 93738A-0 Reagent A 1 mL HI 93738B-0 Dechlorinating Reagent B 1 packet HI 93738C-0 Reagent C 1 mL HI 93738D-0 Reagent D 1 mL

REAGENT SETS

HI 93738-01 Reagents for 100 tests HI 93738-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the described in the

• Fill two graduated mixing cylinders (# 1 & # 2) up to the 25 mL mark with the sample.

Chlorine Dioxide

Method Selection

method using the procedure

section (see page 12).

# 1

25 mL

# 2

25 mL

• Add 0.5 mL of HI 93738A-0 Chlorine Dioxide Reagent to each cylinder (# 1 & # 2), close them and invert several times to mix.

# 1 & # 2

# 1 # 2

Chlorine Dioxide

• Add the content of one packet of HI 93738B-0 Dechlorinating Reagent to one of the two cylinders (# 1), close and invert it several times until it is totally dissolved. This is the blank.

# 1

• Add precisely 0.5 mL of HI 93738C-0 Chlorine Dioxide Reagent to each cylinder (# 1 & # 2), close them and invert several times to mix.

# 1 & # 2

# 1

# 2

• Add 0.5 mL of HI 93738D-0 Chlorine Dioxide Reagent to each cylinder (# 1 & # 2), close them and invert several times to mix. Cylinder # 2 is the reacted sample.

# 1 & # 2

# 1

• Fill a cuvette with 10 mL of the blank (# 1) up to the mark and replace the cap.

# 2

# 1

10 mL

• Place the blank (# 1) into the holder and close the lid.

# 1

Chlorine Dioxide

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for

measurement.

• Fill another cuvette with 10 mL of the reacted sample (# 2)

# 2

up to the mark and replace the cap.

10 mL

# 2

• Insert the sample into the instrument.

• Press Read and the meter will perform the reading. The instrument displays the results in mg/L of

chlorine dioxide.

SAMPLING PROCEDURE

It is recommended to analyze chlorine dioxide samples immediately after collection. Chlorine dioxide samples must be stored in sealed dark glass bottle, with minimal head space. Excessive heat (above 25°C/78°F), agitation and exposure to light must be avoided.

INTERFERENCES

Interferences may be caused by strong oxidants.

Chlorine Dioxide

CHROMIUM VI HIGH RANGE

SPECIFICATIONS

Range 0 to 1000 μg/L Resolution 1 μg/L Accuracy ±5 μg/L ±4% of reading at 25 °C Typical EMC ±1 μg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

Diphenylcarbohydrazide method. The reaction between chromium VI and the reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93723-0 Powder reagent 1 packet

REAGENT SETS

HI 93723-01 Reagents for 100 tests HI 93723-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Chromium VI HR

Method Selection

section (see page 12).

ASTM Manual of Water and Environmental Technology, D1687-92

method using the procedure described in

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette and add the content of one packet of HI 93723-0 reagent. Replace the cap and shake vigorously for about 10 seconds.

Chromium VI HR

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in

μμ

μg/L of chromium VI.

μμ

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in μg/L of Chromate (CrO

¯) and Dichromate (Cr2O

¯).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Vanadium above 1 ppm. However, waiting 10 minutes before reading, the interference is removed Iron above 1 ppm Mercurous and mercuric ions cause slight inhibition of the reaction.

Chromium VI HR

CHROMIUM VI LOW RANGE

SPECIFICATIONS

Range 0 to 300 μg/L Resolution 1 μg/L Accuracy ±1 μg/L ±4% of reading at 25 °C Typical EMC ±1 μg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

Diphenylcarbohydrazide method. The reaction between chromium VI and the reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93749-0 Powder reagent 1 packet

REAGENT SETS

HI 93749-01 Reagents for 100 tests HI 93749-03 Reagents for 300 tests

For other accessories see page 132.

ASTM Manual of Water and Environmental Technology, D1687-92

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for

• Remove the cuvette and add the content of one packet of

Chromium VI LR

the

Method Selection

and replace the cap.

measurement.

HI 93749-0 reagent. Replace the cap and shake vigorously for about 10 seconds.

method using the procedure described in

section (see page 12).

10 mL

• Reinsert the cuvette into the instrument.

μμ

μg/L of chromium VI.

μμ

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in μg/L of Chromate (CrO

¯) and Dichromate (Cr2O

¯).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Vanadium above 1 ppm. However, waiting 10 minutes before reading, the interference is removed. Iron above 1 ppm Mercurous and mercuric ions cause slight inhibition of the reaction.

Chromium VI LR

COLOR OF WATER

SPECIFICATIONS

Range 0 to 500 PCU (Platinum Cobalt Units) Resolution 1 PCU Accuracy ±10 PCU ±5% of reading at 25 °C Typical EMC ± 1 PCU

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the

18th edition

REQUIRED ACCESSORIES

0.45 μm membrane for true color measurement. For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Color of Water

the

Method Selection

method using the procedure described in

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

, Colorimetric Platinum Cobalt method.

10 mL

• Fill one cuvette up to the mark with deionized water and replace

# 1

the cap. This is the blank.

• Place the blank (# 1) into the holder and close the lid.

# 1

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the blank.

Color of Water

• Fill the second cuvette up to the mark with unfiltered sample and replace the cap. This is the apparent color.

• Filter 10 mL of sample through a filter with a 0.45 μm membrane into the third cuvette, up to the 10 mL mark and replace the cap. This is the true color.

10 mL

# 2

# 3

• Insert the apparent color cuvette (# 2) into the instrument and

# 2

close the lid.

• Press Read to start the reading.

• The meter displays the value of apparent color in PCU.

• Remove the cuvette, insert the true color cuvette (# 3) into the

# 3

instrument and ensure that the notch on the cap is positioned securely into the groove.

• Press Read to start the reading. The meter displays the value of true color in PCU.

Color of Water

COPPER HIGH RANGE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the

reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93702-0 Bicinchoninate 1 packet

REAGENT SETS

HI 93702-01 Reagents for 100 tests HI 93702-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

Copper HR

Method Selection

and replace the cap.

section (see page 12).

method using the procedure described in the

EPA method.

The reaction between copper and the bicinchoninate

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

Copper HR

• Remove the cuvette.

• Add the content of one packet of HI 93702-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.

• The instrument displays the results in mg/L of copper.

INTERFERENCES

Interference may be caused by: Silver Cyanide For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Copper HR

COPPER LOW RANGE

SPECIFICATIONS

Range 0 to 1000 μg/L Resolution 1 μg/L Accuracy ±10 μg/L ±5% of reading at 25 °C Typical EMC ±1 μg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the

reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93747-0 Bicinchoninate 1 packet

REAGENT SETS

HI 93747-01 Reagents for 100 tests HI 93747-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Copper LR

Method Selection

method using the procedure described in

section (see page 12).

EPA method.

The reaction between copper and the bicinchoninate

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

Copper LR

• Remove the cuvette.

• Add the content of one packet of HI 93747-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.

• The instrument displays the results in

μμ

μg/L of copper.

μμ

INTERFERENCES

Interference may be caused by: Silver Cyanide For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Copper LR

CYANURIC ACID

SPECIFICATIONS

Range 0 to 80 mg/L Resolution 1 mg/L Accuracy ±1 mg/L ±15% of reading at 25 °C Typical EMC ±1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the turbidimetric method. The reaction between cyanuric acid and the

reagent causes a white suspension in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93722-0 Powder reagent 1 packet

REAGENT SETS

HI 93722-01 Reagents for 100 tests HI 93722-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the described in the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Cyanuric Acid

Method Selection

method using the procedure

section (see page 12).

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

Cyanuric Acid

• Add the content of one packet of HI 93722-0 Cyanuric Acid Reagent. Replace the cap and shake gently for about 10 seconds (dissolution is not complete).

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.

• The instrument displays concentration in mg/L of cyanuric acid.

Cyanuric Acid

FLUORIDE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the

18th edition

causes a red tint in the sample.

REQUIRED REAGENT

Code Description Quantity

HI 93729-0 SPADNS Reagent 4 mL

REAGENT SETS

HI 93729-01 Reagents for 100 tests HI 93729-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Fluoride

Method Selection

method using the procedure described in the

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

, SPADNS method. The reaction between fluoride and the liquid reagent

• Add 2 mL of HI 93729-0 SPADNS Reagent to two cuvettes.

• Fill one of the cuvettes with distilled water up to the mark, replace the cap and invert several times to mix.

• Fill the other cuvette with sample up to the mark, replace the cap and invert several times to mix.

• Place the cuvette with the reacted distilled water (# 1) into the holder and close the lid.

Fluoride

# 1

2 mL

10 mL10 mL

# 2

• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively, wait for two minutes and press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

# 2

• Insert the other cuvette (# 2) with the reacted sample into the instrument.

• Press Read to start reading. The instrument displays the results in mg/L of fluoride.

Note: For wastewater or seawater samples, before performing measurements, distillation is required. For most accurate results, use two graduated pipettes to deliver exactly 8 mL of distilled water and 8 mL of sample.

INTERFERENCES

Interferences may be caused by: Alkalinity (as CaCO3) above 5000 mg/L Aluminum above 0.1 mg/L Iron, ferric above 10 mg/L Chloride above 700 mg/L Phosphate, ortho above 16 mg/L Sodium hexametaphosphate above 1.0 mg/L Sulfate above 200 mg/L Highly colored and turbid samples may require distillation Highly alkaline samples can be neutralized with nitric acid.

Fluoride

CALCIUM HARDNESS

SPECIFICATIONS

Range 0.00 to 2.70 mg/L Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

18th edition,

reddish-violet tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93720A-0 Ca & Mg indicator 0.5 mL HI 93720B-0 Alkali solution 0.5 mL HI 93720C-0 EGTA solution 1 drop

REAGENT SETS

HI 93720-01 Reagents for 100 tests HI 93720-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the described in the

Calcium Hardness

Method Selection

Standard Methods for the Examination of Water and Wastewater,

Calmagite method. The reaction between calcium and reagents causes a

method using the procedure

section (see page 12).

• Rinse a graduated beaker several times with unreacted sample, before filling it to the 50 mL mark with the sample.

• Add 0.5 mL of HI 93720A-0 Calcium indicator solution and swirl to mix.

• Add 0.5 mL of HI 93720B-0 Alkali solution and swirl to mix. Use this solution to rinse 2 cuvettes before filling them up to the 10 mL mark.

Hardness Ca

# 1 # 2

• Add 1 drop of HI 93720C-0 EGTA solution to one cuvette (# 1), replace the cap and invert the cuvette several times to mix. This is the blank.

# 1

• Place the blank (# 1) into the holder and close the lid.

# 1

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the blank and insert the second cuvette (# 2)

# 2

into the instrument.

• Press Read to start the reading. The instrument displays concentration in mg/L of calcium

hardness, as CaCO3.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of Calcium (Ca2+).

Hardness Ca

• Press the Unit key to change the current measurement unit. The results can be converted to French

degrees (°f), German degrees (°dH) and English degrees (°E).

• Press  or  to return to the measurement screen.

Note: This test will detect any calcium contamination in the beaker, measuring syringes or sample cells. To

test cleanliness, repeat the test multiple times until you obtain consistent results.

Note: For better accuracy wash glassware with HCl 6N.

SAMPLE DILUTION

This meter is designed to determine low levels of hardness, typically found in water purification systems. When testing some other sources of water, it is not uncommon to come across levels of hardness that are

greater than the range of this meter. This problem can be overcome through dilution. Dilutions must be performed with hardness-free water or the

readings will be erroneous. A dilution to reduce the level of hardness by a factor of one hundred is performed as follows:

• Fill a 1 mL syringe with the sample.

• Place the syringe in a 50 mL beaker, making sure that the beaker is clean and empty, and inject

0.5 mL into the beaker.

• Fill the beaker up to the 50 mL mark with hardness-free water.

INTERFERENCES

Interference may be caused by excessive amounts of heavy metals.

Hardness Ca

MAGNESIUM HARDNESS

SPECIFICATIONS

Range 0.00 to 2.00 mg/L Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Typical EMC ±0.02 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

18th edition,

causes a reddish-violet tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93719A-0 Mg indicator 0.5 mL HI 93719B-0 Alkali solution 0.5 mL HI 93719C-0 EDTA solution 1 drop HI 93719D-0 EGTA solution 1 drop

REAGENT SETS

HI 93719-01 Reagents for 100 tests HI 93719-03 Reagents for 300 tests

For other accessories see page 132.

Standard Methods for the Examination of Water and Wastewater,

EDTA colorimetric method. The reaction between magnesium and reagents

MEASUREMENT PROCEDURE

• Select the described in the

• Rinse a graduated beaker several times with unreacted sample, before filling it to the 50 mL mark with the sample.

• Add 0.5 mL of HI 93719A-0 Magnesium indicator solution, then swirl to mix.

• Add 0.5 mL of HI 93719B-0 Alkali solution and swirl to mix. Use this solution to rinse 2 cuvettes.

Magnesium Hardness

Method Selection

method using the procedure

section (see page 12).

59 Hardness Mg

• Fill both cuvettes up to the 10 mL mark.

• Add 1 drop of HI 93719C-0 EDTA solution to one cuvette (# 1), replace the cap and invert the cuvette several times to mix. This is the blank.

• Add 1 drop of HI 93719D-0 EGTA solution to the second cuvette (# 2), replace the cap and invert the cuvette several times to mix. This is the sample.

10 mL

# 1

# 2

10 mL

# 2

# 1

• Place the blank (# 1) into the holder and close the lid.

# 1

• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

# 2

• Remove the blank (# 1), insert the sample (# 2) into the instrument, and close the lid.

• Press Read to start the reading. The instrument displays concentration in mg/L of magnesium

hardness, as CaCO3.

Hardness Mg

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of Magnesium (Mg2+).

• Press the Unit key to change the current measurement unit. The results can be converted to French

degrees (°f), German degrees (°dH) and English degrees (°E).

• Press  or  to return to the measurement screen.

Note: This test will detect any magnesium contamination in the beakers, measuring syringes or sample cells. To test cleanliness, repeat the test multiple times until you obtain consistent results.

SAMPLE DILUTION

This meter is designed to determine hardness typically found in water purification systems. In order to measure samples with high hardness, follow dilution procedure explained on page 58 (Ca Hardness).

INTERFERENCES

Interference may be caused by excessive amounts of heavy metals.

Hardness Mg

HYDRAZINE

SPECIFICATIONS

Range 0 to 400 μg/L Resolution 1 μg/L Accuracy ±4% of full scale reading at 25 °C Typical EMC ±2 μg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the

D1385-88,

and the liquid reagent causes a yellow tint in the sample.

REQUIRED REAGENT

Code Description Quantity

HI 93704-0 Liquid Reagent 24 drops

REAGENT SETS

HI 93704-01 Reagents for 100 tests HI 93704-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the in the

Hydrazine

Method Selection

method using the procedure described

section (see page 12).

ASTM Manual of Water and Environmental Technology, method

p-Dimethylaminobenzaldehyde method. The reaction between hydrazine

10 mL

• Fill one cuvette up to the mark with 10 mL of distilled water.

• Place the cap, insert the cuvette # 1 into the holder and

# 1

close the lid.

• Press the Blank key to start adjusting the light level. The display will show “Blank done” when the meter is ready to take a zero measurement.

• Fill a second cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Hydrazine

10 mL

# 2

• Add 12 drops of the HI 93704-0 reagent to each cuvette. Replace the caps and shake gently to mix (about 30 seconds).

# 1

# 2

# 1

• Place the blank (#1) into the holder and close the lid.

• Press Timer and the display will show the countdown prior to zeroing the blank. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the blank.

# 2

• Insert the cuvette with the reacted sample (# 2) into the instrument and close the lid.

• Press Read to start the reading. The instrument displays concentration in

INTERFERENCES

Interference may be caused by: Highly colored samples Highly turbid samples Aromatic amines

μμ

μg/L of hydrazine.

μμ

Hydrazine

IODINE

SPECIFICATIONS

Range 0.0 to 12.5 mg/L Resolution 0.1 mg/L Accuracy ±0.1 mg/L ±5% of reading at 25 °C Typical EMC ±0.1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

18th edition

tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93718-0 DPD Reagent 1 packet

REAGENT SETS

HI 93718-01 Reagents for 100 tests HI 93718-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Iodine

the

Method Selection

method using the procedure described in

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

, DPD method. The reaction between iodine and the reagent causes a pink

10 mL

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cap and add the content of one packet of HI 93718-0 DPD reagent. Replace the cap and shake gently for about 30 seconds to dissolve most of the reagent.

Iodine

• Reinsert the cuvette into the instrument.

• The instrument displays concentration in mg/L of iodine.

INTERFERENCES

Interference may be caused by: Bromine, Chlorine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately

2 minutes after adding the reagent. In case of water with alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3,

the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Iodine

IRON HIGH RANGE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±2% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

The reaction between iron and reagents causes an orange tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93721-0 Powder Reagent 1 packet

REAGENT SETS

HI 93721-01 Reagents for 100 tests HI 93721-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the in the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Iron HR

method using the procedure described

Method Selection

section (see page 12).

EPA Phenantroline method 315B,

for natural and treated waters.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” the meter is zeroed and ready for measurement.

• Remove the cuvette and add the content of one packet of HI 93721-0 reagent. Replace the cap and shake until dissolution is complete.

Iron HR

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and press Read. When the timer ends the meter will perform the reading.

• The instrument displays concentration in mg/L of iron.

INTERFERENCES

Interference may be caused by: Molybdate Molybdenum above 50 ppm Calcium above 10000 ppm (as CaCO3) Magnesium above 100000 ppm (as CaCO3) Chloride above 185000 ppm.

Iron HR

IRON LOW RANGE

SPECIFICATIONS

Range 0 to 400 μg/L Resolution 1 μg/L Accuracy ±10 μg/L ±8% of reading at 25 °C Typical EMC ±1 μg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the TPTZ Method. The reaction between iron and the reagent causes a

violet tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93746-0 TPTZ Reagent 2 packets

REAGENT SETS

HI 93746-01 Reagents for 50 tests HI 93746-03 Reagents for 150 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the the

• Fill one graduated mixing cylinder up to the 25 mL mark with deionized water.

Iron LR

method using the procedure described in

Method Selection

section (see page 12).

25 mL

• Add the content of one packet of HI 93746-0 TPTZ reagent, close the cylinder and shake vigorously for 30 seconds. This is the blank.

• Fill a cuvette with 10 mL of the blank up to the mark and replace the cap.

• Place the cuvette into the holder and close the lid.

Iron LR

10 mL

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

25 mL

• Fill another graduated mixing cylinder up to the 25 mL mark with the sample.

• Add the content of one packet of HI 93746-0 TPTZ reagent, close the cylinder and shake vigorously for 30 seconds. This is the reacted sample.

• Fill a cuvette with 10 mL of the reacted sample up to the mark and replace the cap.

10 mL

• Insert the sample into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in

μμ

μg/L of iron.

μμ

Iron LR

INTERFERENCES

Interference may be caused by: Cadmium above 4.0 mg/L

Chromium Chromium Cobalt above 0.05 mg/L Copper above 0.6 mg/L Cyanide above 2.8 mg/L Manganese above 50.0 mg/L Mercury above 0.4 mg/L Molybdenum above 4.0 mg/L Nickel above 1.0 mg/L Nitrite ion above 0.8 mg/L Sample pH should be between 3 and 4 to avoid developed color to fade or turbidity formation.

above 0.25 mg/L

above 1.2 mg/L

Iron LR

MAGNESIUM

SPECIFICATIONS

Range 0 to 150 mg/L Resolution 5 mg/L Accuracy ±5 mg/L ±3% of reading at 25 °C Light Source Tungsten lamp with narrow band interference filter @ 466 nm Method Adaptation of the Calmagite method

REQUIRED REAGENTS

Code Description Quantity

HI 93752A-0 Mg Magnesium Buffer Reagent 1 mL HI 93752B-0 Mg Magnesium Indicator Reagent 9 mL

REAGENT SETS

HI 937520-01 Reagents for 50 tests HI 937520-03 Reagents for 150 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

Note: for sample preparation follow the COLORED OR TURBID SAMPLES

procedure on page 17.

• Select the

Method Selection

Magnesium

method using the procedure described in the

section (see page 12).

• Using one 1 mL syringe add exactly 1.00 mL of HI 93752A-0 Mg Buffer reagent to the cuvette and use the pipette to fill the cuvette up to the 10 mL mark with the HI 93752B-0 Mg Indicator reagent.

• Replace the cap and invert several times to mix.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Magnesium

• Remove the cuvette.

0.5 mL

of sample

• Using the other 1 mL syringe, add to the cuvette exactly 0.5 mL of sample.

Note: Do not mix up the two syringes!

• Replace the cap and invert several times to mix.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 15 seconds and press Read. When the timer ends the meter will perform the reading.

• The instrument displays the results in mg/L of magnesium (Mg2+).

INTERFERENCES

Interferences may be caused by: Acidity (as CaCO3) above 1000 mg/L Alkalinity (as CaCO3) above 1000 mg/L Calcium (Ca2+) above 200 mg/L Iron must be absent Aluminum must be absent Copper must be absent

Magnesium

MANGANESE HIGH RANGE

SPECIFICATIONS

Range 0.0 to 20.0 mg/L Resolution 0.1 mg/L Accuracy ±0.2 mg/L ±3% of reading at 25 °C Typical EMC ±0.1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

18th edition,

a pink tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93709A-0 Citrate 1 packet HI 93709B-0 Sodium periodate 1 packet

REAGENT SETS

HI 93709-01 Reagents for 100 tests HI 93709-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette with 10 mL of unreacted sample (up to the

Manganese HR

in the

Method Selection

mark) and replace the cap.

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

Periodate method. The reaction between manganese and reagents causes

method using the procedure described

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add the content of one packet of HI 93709A-0 Citrate reagent. Replace the cap and invert to mix with gently movements for 2 minutes.

Manganese HR

• Add the content of one packet of HI 93709B-0 Sodium Periodate reagent. Replace the cap and invert to mix with gently movements for 2 minutes.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 1 minute and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of manganese.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of potassium permanganate (KMnO4) and permanganate (MnO4¯).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Calcium above 700 mg/L Chloride above 70000 mg/L Iron above 5 mg/L Magnesium above 100000 mg/L.

Manganese HR

MANGANESE LOW RANGE

SPECIFICATIONS

Range 0 to 300 μg/L Resolution 1 μg/L Accuracy ±10 μ/L ±3% of reading at 25 °C Typical EMC ±1 μg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the PAN Method. The reaction between manganese and the reagents

causes an orange tint in the sample.

REQUIRED REAGENT

Code Description Quantity

HI 93748A-0 Ascorbic acid 2 packets HI 93748B-0 Alkaline-cyanide sol. 0.40 mL HI 93748C-0 0.1% PAN indicator 2 mL HI 93703-51 Dispersing Agent 4-6 drops

REAGENT SETS

HI 93748-01 Reagents for 50 tests HI 93748-03 Reagents for 150 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the page 12).

Manganese LR

method using the procedure described in the

Method Selection

section (see

• Fill one cuvette with 10 mL of deionized water (up to the mark).

• Fill a second cuvette with 10 mL of sample (up to the mark).

• Add the content of one packet of HI 93748A-0 Ascorbic acid to each cuvette, replace the caps and shake gently until completely dissolved.

10 mL

Manganese LR

• Add 0.2 mL of the HI 93748B-0 Alkaline-cyanide reagent solution to each cuvette, replace the caps and invert gently to mix for about 30 seconds.

• Add 1 mL of the HI 93748C-0 0.1% PAN indicator solution to each cuvette, replace the caps and shake gently.

• Add 3 drops of Dispersing Agent (HI 93703-51) to each cuvette, replace the caps and invert gently to mix for about 30 seconds.

• Place the cuvette with the reacted deionized water (blank) into the holder and close the lid.

• Press Timer and the display will show the countdown prior to zeroing the blank. Alternatively wait for 2 minutes and then press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Manganese LR

• Insert the second cuvette with the reacted sample into the instrument.

• Press Read to start the reading. The instrument displays the results in

μμ

μg/L of manganese.

μμ

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in μg/L of potassium permanganate (KMnO4) and

permanganate (MnO4¯).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Aluminum above 20 mg/L Cadmium above 10 mg/L Calcium above 200 mg/L as CaCO Cobalt above 20 mg/L Copper above 50 mg/L Iron above 10 mg/L Lead above 0.5 mg/L Magnesium above 100 mg/L as CaCO Nickel above 40 mg/L Zinc above 15 mg/L.

Manganese LR

MOLYBDENUM

SPECIFICATIONS

Range 0.0 to 40.0 mg/L Resolution 0.1 mg/L Accuracy ±0.3 mg/L ±5% of reading at 25 °C Typical EMC ±0.1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the mercaptoacetic acid method. The reaction between molybdenum and

the reagents causes a yellow tint in the sample.

REQUIRED REAGENT

Code Description Quantity

HI 93730A-0 Reagent A 1 packet HI 93730B-0 Reagent B 1 packet HI 93730C-0 Reagent C 1 packet

REAGENT SETS

HI 93730-01 Reagents for 100 tests HI 93730-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the described in the

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

Molybdenum

Method Selection

method using the procedure

section (see page 12).

10 mL

• Place cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

78Molybdenum

• Fill one graduated mixing cylinder up to the 25 mL mark with the sample.

• Add the content of one packet of HI 93730A-0 molybdenum reagent, close the cylinder and invert it several times until completely dissolved.

• Add the content of one packet of HI 93730B-0 molybdenum reagent to the cylinder, close and invert it several times until completely dissolved.

• Add the content of one packet of HI 93730C-0 molybdenum reagent to the cylinder, close and shake it vigorously.

25 mL

• Fill an empty cuvette with 10 mL of sample up to the mark and replace the cap.

• Insert the cuvette into the instrument.

10 mL

Molybdenum

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for five minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of molybdenum.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of molybdate (MoO

¯) and sodium molybdate

(Na2MoO4).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Aluminum above 50 mg/L Chromium above 1000 mg/L Copper above 10 mg/L Iron above 50 mg/L Nickel above 50 mg/L Nitrite, as NO2¯ Sulfate above 200 mg/L Highly buffered samples or with extreme pH may exceed the buffering capacity of the reagents.

Molybdenum

NICKEL HIGH RANGE

SPECIFICATIONS

Range 0.00 to 7.00 g/L Resolution 0.01 g/L Accuracy ±0.07 ±4% of reading at 25 °C Typical EMC ±0.02 g/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the photometric method. The reaction between nickel and the reagent

causes a blue tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93726-0 Powder reagent 1 packet

REAGENT SETS

HI 93726-01 Reagents for 100 tests HI 93726-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Nickel HR

method using the procedure described in the

Method Selection

section (see page 12).

• Fill the cuvette up to the mark with 10 mL of unreacted sample and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Nickel HR

• Remove the cuvette and add the content of one packet of HI 93726-0 reagent. Replace the cap and shake gently until completely dissolved.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 1 minute and press Read. When the timer ends the meter will perform the reading.

• The instrument displays concentration in g/L of nickel.

INTERFERENCES

Interference may be caused by copper.

Nickel HR

NICKEL LOW RANGE

SPECIFICATIONS

Range 0.000 to 1.000 mg/L Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±7% of reading at 25 °C Typical EMC ±0.001 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the PAN method. The reaction between nickel and the reagents causes

an orange tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93740A-0 Phthalate-phosphate 2 packets HI 93740B-0 0.3% PAN indicator 2 mL HI 93740C-0 EDTA 2 packets HI 93703-51 Dispersing Agent 4-6 drops (only when necessary, see note)

REAGENT SETS

HI 93740-01 Reagents for 50 tests HI 93740-03 Reagents for 150 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the described in the

Note: for best results perform your tests between 20-24°C.

Nickel LR

method using the procedure

Method Selection

section (see page 12).

• Fill one graduated beaker with 25 mL of deionized water (blank) and another one with 25 mL of sample.

• Add the content of one packet of HI 93740A-0 Phthalate-phosphate reagent to each beaker. Cap and swirl gently until the reagent is dissolved.

Note: If sample contains iron (Fe3+), it is important that all powder be dissolved completely before continuing with following step.

• Add 1 mL of HI 93740B-0 0.3% PAN solution to each beaker, cap and swirl to mix.

83 Nickel LR

• Press Timer and the display will show a countdown prior to adding reagent C or, alternatively, wait

for 15 minutes. Add one packet of HI 93740C-0 EDTA reagent to each beaker, cap and swirl to mix until completely dissolved.

• Fill one cuvette up to the mark with 10 mL of the blank.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

• Fill a second cuvette up to the mark with 10 mL of the reacted sample.

10 mL

• Insert the second cuvette into the instrument.

• Press Read to start the reading. The instrument displays the results in mg/L of nickel.

Note: a temperature above 30°C may cause turbidity. In this case, before zeroing and taking readings, add 2-3 drops of Dispersing Agent (HI 93703-51) to each cuvette and swirl until turbidity is removed.

Nickel LR

INTERFERENCES

Interference may be caused by: Co2+ must not be present Fe2+ must not be present Al3+ above 32 mg/L Ca2+ above 1000 mg/L (as CaCO3) Cd2+ above 20 mg/L Cl¯ above 8000 mg/L Cr3+ above 20 mg/L Cr6+ above 40 mg/L Cu2+ above 15 mg/L F¯ above 20 mg/L Fe3+ above 10 mg/L K+ above 500 mg/L Mg2+ above 400 mg/L Mn2+ above 25 mg/L Mo6+ above 60 mg/L Na+ above 5000 mg/L Pb2+ above 20 mg/L Zn2+ above 30 mg/L

Nickel LR

NITRATE

SPECIFICATIONS

Range 0.0 to 30.0 mg/L Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Typical EMC ±0.1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. The reaction between nitrate and the

reagent causes an amber tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93728-0 Powder reagent 1 packet

REAGENT SETS

HI 93728-01 Reagents for 100 tests HI 93728-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Method Selection

• Using the pipette, fill the cuvette with 6 ml of sample, up to half of its height, and replace the cap.

Nitrate

method using the procedure described in the

section (see page 12).

6 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette and add the content of one packet of HI 93728-0 reagent.

Nitrate

• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement.

• Reinsert the cuvette into the instrument, taking care not to shake it.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 4 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of nitrate-nitrogen.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of nitrate (NO3¯).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Ammonia and amines, as urea and primary aliphatic amines Chloride above 100 ppm Chlorine above 2 ppm Copper Iron(III) Strong oxidizing and reducing substances Sulfide must be absent

Nitrate

NITRITE HIGH RANGE

SPECIFICATIONS

Range 0 to 150 mg/L Resolution 1 mg/L Accuracy ±4 mg/L ±4% of reading at 25 °C Typical EMC ±1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the Ferrous Sulfate method. The reaction between nitrite and the

reagent causes a greenish-brown tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93708-0 Powder reagent 1 packet

REAGENT SETS

HI 93708-01 Reagents for 100 tests HI 93708-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Method Selection

• Fill the cuvette up to the mark with 10 mL of unreacted sample and replace the cap.

Nitrite HR

method using the procedure described in the

section (see page 12).

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

Nitrite HR

• Add the content of one packet of HI 93708-0 reagent. Replace the cap and shake gently until completely dissolved.

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 10 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of nitrite.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of nitrogen-nitrite (NO2¯-N) and sodium nitrite (NaNO2).

• Press  or  to return to the measurement screen.

Nitrite HR

NITRITE LOW RANGE

SPECIFICATIONS

Range 0.00 to 1.15 mg/L Resolution 0.01 mg/L Accuracy ±0.06 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the

the reagent causes a pink tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93707-0 Powder reagent 1 packet

REAGENT SETS

HI 93707-01 Reagents for 100 tests HI 93707-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

• Fill the cuvette up to the mark with 10 mL of unreacted

Nitrite LR

method using the procedure described in

the

Method Selection

sample (up to the mark) and replace the cap.

section (see page 12).

EPA Diazotization method 354.1

. The reaction between nitrite and

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add the content of one packet of HI 93707-0 reagent. Replace the cap and shake gently for about 15 seconds.

Nitrite LR

• Reinsert the cuvette into the instrument.

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result in mg/L of nitrogen-nitrite (NO2¯-N) and sodium nitrite (NaNO2).

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by the following ions: ferrous, ferric, cupric, mercurous, silver, antimonious, bismuth, auric, lead, metavanadate and chloroplatinate. Strongly reducing and oxidizing reagents. High levels of nitrate (above 100 mg/L) could yield falsely high readings due to a minute amount of

reduction to nitrite that could occur at these levels.

Nitrite LR

DISSOLVED OXYGEN

SPECIFICATIONS

Range 0.0 to 10.0 mg/L Resolution 0.1 mg/L Accuracy ±0.4 mg/L ±3% of reading at 25 °C Typical EMC ± 0.1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the

18th edition,

and the reagents causes a yellow tint in the sample.

REQUIRED REAGENTS

Code Description Quantity

HI 93732A-0 Reagent A 5 drops HI 93732B-0 Reagent B 5 drops HI 93732C-0 Reagent C 10 drops

REAGENT SET

HI 93732-01 Reagents for 100 tests HI 93732-03 Reagents for 300 tests

For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Dissolved Oxygen

the

Method Selection

section (see page 12).

Standard Methods for the Examination of Water and Wastewater,

Azide modified Winkler method. The reaction between dissolved oxygen

method using the procedure described in

• Fill one 60 mL glass bottle completely with the unreacted sample.

• Replace the cap and ensure that a small part of the sample spills over.

• Remove the cap and add 5 drops of HI 93732A-0 and 5 drops of HI 93732B-0.

• Add more sample, to fill the bottle completely. Replace the cap again and ensure that a part of the sample spills over. This is to make sure that no air bubbles have been trapped inside, which could alter the reading.

• Invert several times the bottle. The sample becomes orange-yellow and a flocculent agent will appear.

Dissolved Oxygen

• Let the sample stand and the flocculent agent will start to settle.

• After approximately 2 minutes, when the upper half of the bottle becomes limpid, add 10 drops of HI 93732C-0.

• Replace the cap and invert the bottle until the settled flocculent dissolves completely. The sample is ready for measurement when it is yellow and completely limpid.

• Fill the cuvette up to the mark with 10 mL of the unreacted (original) sample, and replace the cap. This is the blank.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

• Remove the cuvette.

• Fill another cuvette up to the mark with 10 mL of the reacted sample and replace the cap.

• Reinsert the cuvette into the instrument.

• Press Read to start the reading. The instrument will display the results in mg/L of dissolved oxygen.

INTERFERENCES

Interferences may be caused by reducing and oxidizing materials.

Dissolved Oxygen

OXYGEN DEMAND, CHEMICAL HIGH RANGE

SPECIFICATIONS

Range 0 to 15000 mg/L COD Resolution 10 mg/L Accuracy ±220 @ 10000 mg/L Typical EMC ±10 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 610 nm Method Adaptation of the USEPA 410.4 approved method for the COD determination on

surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of chromic ion formed is determined.

REQUIRED REAGENTS

Description Q.ty/test Q.ty/set

Reagent Vial 1 vial 25 vials Deionized Water 0.2 mL optional

Note: Store the unused vials in their container in a cool and dark place.

REAGENT SET

HI 93754C-25 Reagents for up to 25 tests

REQUIRED ACCESSORIES

HI 839800-01 Hanna reactor (115 VAC) HI 839800-02 Hanna reactor (230 VAC) HI 740216 Test tube cooling rack (25 holes) HI 740217 Laboratory bench safety shield

For other accessories see page 132.

MEASUREMENT PROCEDURE

Before using the reagent kit carefully read all the instructions and the Material Safety Data Sheet (MSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so

may result in serious injury to the operator.

Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may be used more than once. The blank vial is stable for several months (room temperature). For most accurate measurement, run a blank for each set of measurements and always use the same lot of reagents for blank and samples.

• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender.

• Preheat the Hanna Reactor HI 839800 to 150 °C (302°F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly explosive atmosphere.

• Remove the cap from two Reagent Vials.

• Add exactly 0.2 mL of sample to one vial (sample vial), and 0.2 mL of deionized water to the other vial (blank vial), while keeping the vials at a 45-degree angle. Replace the cap tightly and mix by inverting each vial a couple of times.

Warning: the vials will become very hot during mixing, be careful when handling them.

• Insert the vials into the reactor and heat them for 2 hours at 150°C.

• At the end of the digestion period switch off the reactor. Wait for twenty

minutes to allow the vials to cool to about 120°C.

• Invert each vial several times while still warm, then place them in the test tube rack.

Warning: the vials are still hot, be careful when handling them.

• Leave the vials in the tube rack to cool to room temperature. Do not shake

or invert them anymore otherwise the samples may become turbid.

• Select the

HR (COD)

procedures described in the

Selection

• Place the COD vial adapter in the cuvet holder and ensure that the adapter is well fit inside.

• Place the blank vial into the holder and push it completely down.

• Press the Zero key and the instrument will perform a zero sequence. If the zero sequence was successfully done, the display will show “-0.0-”. Now the meter is zeroed and ready for measurement.

• Remove the blank vial.

• Place the sample vial into the holder and push it completely down.

Oxygen Demand, Chemical

method following one of the

Method

section (see page 12).

• Press Read and the instrument will perform the reading.

• The instrument directly displays concentration in mg/L of oxygen demand.

INTERFERENCES

Interference may be caused by: Chloride (Cl¯) above 20000 mg/L.

Samples with higher chloride concentration should be diluted.

OXYGEN DEMAND, CHEMICAL MEDIUM RANGE

SPECIFICATIONS

Range 0 to 1500 mg/L COD Resolution 1 mg/L Accuracy ±22 @ 1000 mg/L Typical EMC ±1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 610 nm Method Adaptation of the USEPA 410.4 approved method for the COD determination on

surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of chromic ion formed is determined.

REQUIRED REAGENTS

Description Q.ty/test Q.ty/set

Reagent Vial 1 vial 25 vials Deionized Water 2.0 mL optional

Note: Store the unused vials in their container in a cool and dark place.

REAGENT SET

HI 93754B-25 Reagents for up to 25 tests

REQUIRED ACCESSORIES

HI 839800-01 Hanna reactor (115 VAC) HI 839800-02 Hanna reactor (230 VAC) HI 740216 Test tube cooling rack (25 holes) HI 740217 Laboratory bench safety shield

For other accessories see page 132.

MEASUREMENT PROCEDURE

Before using the reagent kit carefully read all the instructions and the Manual Safety Data Sheet (MSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so may result in serious injury to the operator.

• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender.

• Remove the cap from two Reagent Vials.

• Add exactly 2.0 mL of sample to one vial (sample vial), and 2.0 mL of deionized water to the other vial (blank vial), while keeping the vials at a 45-degree angle. Replace the cap tightly and mix by inverting each vial a couple of times.

Warning: the vials wil become very hot during mixing, be careful when handling them.

• Insert the vials into the reactor and heat them for 2 hours at 150°C.

• At the end of the digestion period switch off the reactor. Wait for twenty

minutes to allow the vials to cool to about 120°C.

• Invert each vial several times while still warm, then place them in a test tube rack.

Warning: the vials are still hot, be careful when handling them.

• Leave the vials in the tube rack to cool to room temperature. Do not shake

or invert them, the samples may become turbid.

• Select the

MR (COD)

procedures described in the

Selection

Oxygen Demand, Chemical

method following one of the

Method

section (see page 12).

• Place the COD vial adapter in the cuvet holder and ensure that the adapter is well fit inside.

• Place the blank vial into the holder and push it completely down.

• Remove the blank vial.

• Place the sample vial into the holder and push it completely down.

• Press Read and the instrument will perform the reading.

• The instrument displays concentration in mg/L of oxygen demand on the LCD.

INTERFERENCES

Interference may be caused by: Chloride (Cl¯) above 2000 mg/L.

Samples with higher chloride concentration should be diluted.

COD MR

OXYGEN DEMAND, CHEMICAL LOW RANGE

SPECIFICATIONS

Range 0 to 150 mg/L COD Resolution 1 mg/L Accuracy ±4 @ 150 mg/L Typical EMC ±1 mg/L

Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Method Adaptation of the USEPA 410.4 approved method for the COD determination on

surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of remaining dichromate is determined.

REQUIRED REAGENTS

Description Q.ty/test Q.ty/set

Reagent Vial 1 vial 25 vials Deionized Wat+er 2.0 mL optional

Note: Store the unused vials in their container in a cool and dark place.

REAGENT SET

HI 93754A-25 Reagents for up to 25 tests

REQUIRED ACCESSORIES

HI 839800-01 Hanna reactor (115 VAC) HI 839800-02 Hanna reactor (230 VAC) HI 740216 Test tube cooling rack (25 holes) HI 740217 Laboratory bench safety shield

For other accessories see page 132.

MEASUREMENT PROCEDURE

Before using the reagent kit carefully read all the instructions and the Material Safety Data Sheet (MSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so may result in serious injury to the operator.

COD LR

100

Hanna instruments HI 83099 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual