Hamilton Microlab FAME Service Manual

M i c r o l a b F . A . M . E .

S e r v i c e M A N U A L

Version 2.0

P/N 610015

Important Notice:

•Reproduction of any part of this manual in any form whatsoever without the express written consent of Hamilton Bonaduz AG is forbidden.

•The contents of this manual are subject to change without notice.

•All efforts have been made to ensure the accuracy of the contents of this manual. However, should any errors be detected, Hamilton Bonaduz AG would greatly appreciate being informed of them.

•The above notwithstanding, Hamilton Bonaduz AG can assume no responsibility for any errors in this manual or their consequences.

•Copyright ã 1996 Hamilton Bonaduz AG, Bonaduz Switzerland. All Rights Reserved.

•Microlab is a registered trademark of Hamilton Bonaduz AG.

•IBM, OS/2 DATABASE 2 and DB2 are registered trademarks of International Business Machines Corporation.

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To:	Hamilton Bonaduz AG	Dealer: ................................................................
	Postfach 26	.............................................................................
	CH-7402 Bonaduz/Switzerland
	Fax: [phone]	.............................................................................
	*After 16.4.96: [phone]
		Service Technician: .............................................
Attn:	Sales Documentation (VD)

Service Manual Registration

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Please complete and send or fax this form to Hamilton Bonaduz AG.

Instrument	Microlab F.A.M.E.
Service Manual p/n	610 015
Manual Registration No: ...................................

I hereby confirm that I have received the above mentioned manual.

Service Technician

Date: .......................................

Signature: ........................................................

Comments: ...................................................................................................................................................................................................................

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——— ML F.A.M.E. Service Manual V2.0 ————————————————————————————————————————— Table of Contents ———

Table of Contents

Chapter	1	SYSTEM .........................................................................................................	1-1
	1.1	ML F.A.M.E. Instrument ...........................................................................	1-1
	1.1.1	General Information ............................................................................................	1-1
		Location of Warning and Attention Labels ..................................................	1-2
	1.1.2	Service Concept ...................................................................................................	1-4
	1.1.2.1	General Information ............................................................................................	1-4
	1.1.2.2	ML F.A.M.E. Service Manual ...........................................................................	1-4
	1.1.2.2.1	Description of text icons ....................................................................................	1-5
	1.1.2.2.2	Updating the Service Manual ..........................................................................	1-5
	1.1.2.2.3	Service Bulletins ..................................................................................................	1-5
	1.1.2.2.4	Service Part Classifications .............................................................................	1-5
	1.1.2.2.5	Service Assemblies ............................................................................................	1-6
	1.1.2.2.6	ML F.A.M.E. instruments with CE conformity ............................................	1-6
	1.1.2.2.7	Pin coding for PCBs ...........................................................................................	1-7
	1.1.2.2.8	Connector numbering ........................................................................................	1-7
	1.1.2.3	Feedback (Quarterly Service Call Statistics) .............................................	1-9
	1.1.2.4	Parts Return Tag ..................................................................................................	1-9
		Material Return Good Authorization .............................................................	1-9
	1.1.2.5	Technical Data Sheet .........................................................................................	1-9
	1.1.2.6	Service Kit ...........................................................................................................	1-10
	1.1.2.6.1	Service Kit Spare Parts List ..........................................................................	1-10
	1.1.2.6.2	Service PCB (to be used with Diagnostic Master) ...............................	1-13
	1.1.3	F.A.M.E. Description ........................................................................................	1-15
	1.1.3.1	Purpose of the system ....................................................................................	1-16
	1.1.3.2	General Operation Procedure ......................................................................	1-16
	1.1.3.3	F.A.M.E configurations ....................................................................................	1-17
	1.1.3.3.1	Overview ..............................................................................................................	1-17
	1.1.3.3.2	Configuration 16/20 .........................................................................................	1-19
	1.1.3.3.3	Configuration 16/30 .........................................................................................	1-27
	1.1.3.3.4	Configuration 24/20 .........................................................................................	1-35
	1.1.3.3.5	Configuration 24/30 .........................................................................................	1-43
	1.1.3.3.6	OEM specific Part Numbers .........................................................................	1-51
	1.1.3.3.7	Exchange Modules and Exchange Module Parts Lists ......................	1-52
	1.1.3.3.8	Expansion Modules and Expansion Module Parts Lists ....................	1-55
	1.1.3.3.9	Shipping Kits ......................................................................................................	1-67
	1.1.4	Microlab F.A.M.E. Installation .......................................................................	1-69

p/n 610 015

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1.1.4.1	Workplace environment ..................................................................................	1-69
1.1.4.2	Unpacking Microlab F.A.M.E. .......................................................................	1-71
	Adjust chassis to instrument configuration ..............................................	1-72
1.1.4.3	Instrument assembly .......................................................................................	1-74
1.1.4.3.1	Installing INCUBATOR Module ...................................................................	1-74
1.1.4.3.2	Installing WASHER/DISPENSER Module ...............................................	1-76
1.1.4.3.3	Installing ENTRY Module ..............................................................................	1-78
1.1.4.3.4	Installing END Module ....................................................................................	1-79
1.1.4.3.5	Installing Main Shuttle Rail & Panels ........................................................	1-80
1.1.4.3.6	Installing Container Stack ..............................................................................	1-86
1.1.4.3.7	Connecting Tubing and Cables ...................................................................	1-87
1.1.4.3.8	Installing Reagent Container Carousel .....................................................	1-90
1.1.4.3.9	Computer Installation ......................................................................................	1-91
1.1.4.3.10	Electrical Installation ........................................................................................	1-92
1.1.4.3.11	Software Installation ........................................................................................	1-92
1.1.4.3.12	Login and Access .............................................................................................	1-92
1.1.4.3.13	System Start-up and Shut-down .................................................................	1-93
1.1.4.3.14	Downloading Firmware to Instrument .......................................................	1-94
1.1.4.3.15	Installing Filter Case ........................................................................................	1-96
1.1.4.3.16	Loading Check Plate into Instrument ........................................................	1-97
1.1.4.3.17	Instrument Calibration .....................................................................................	1-98
	Rotor Calibration Pin .......................................................................................	1-99
1.1.5	Instrument Disassembly ..............................................................................	1-101
1.1.5.1	Instrument shut down ...................................................................................	1-101
1.1.5.1.1	Removing the Photometer Filter Case ..................................................	1-101
1.1.5.1.2	Removing MTPs and Check Plates ........................................................	1-102
1.1.5.1.3	Decontamination ............................................................................................	1-102
1.1.5.1.4	Draining the washer system ......................................................................	1-103
1.1.5.2	Preparing Modules for Disassembly .......................................................	1-103
1.1.5.3	Disassembly ....................................................................................................	1-105
1.1.5.3.1	Removing Container Stack ........................................................................	1-106
1.1.5.3.2	Removing Panels & Main Shuttle Rail ...................................................	1-108
1.1.5.3.3	Handling and Removal of Modules ..........................................................	1-111
1.1.6	Packing F.A.M.E. for Shipment ..................................................................	1-117
1.1.7	Exchange Modules ........................................................................................	1-125
1.1.8	Expansion Modules .......................................................................................	1-126
1.1.9	Decontamination ............................................................................................	1-127
1.1.10	Maintenance ....................................................................................................	1-127
1.1.10.1	General maintenance ...................................................................................	1-127
1.1.10.2	Half Yearly Maintenance / Service ..........................................................	1-128

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	1.1.10.2.1	Tasks ..................................................................................................................	1-128
	1.1.10.2.2	Running Macros for Half Yearly Maintenance/Service ....................	1-128
	1.1.10.2.3	Macros Available for Maintenance/Service ..........................................	1-129
	1.1.10.3	Preventive maintenance ..............................................................................	1-130
	1.2	Microlab AT / AT plus / AT plus 2 ...........................................	1-135
	1.3	F.A.M.E. Software .................................................................................	1-139
	1.3.1	Personal Computer Requirements ..........................................................	1-139
	1.3.2	ML F.A.M.E. System Requirements ........................................................	1-139
	1.3.3	Software Requirements ...............................................................................	1-139
	1.3.4	Microlab F.A.M.E. Software Specifications ...........................................	1-140
	1.3.5	Connections to Sample Preparation Instruments ..............................	1-140
	1.3.6	Overview ...........................................................................................................	1-141
	1.3.7	Diagnostic Master ..........................................................................................	1-142
	1.3.8	How to run macros ........................................................................................	1-143
	1.4	Totally Integrated ML F.A.M.E System ...............................	1-147
Chapter	2	ENTRY MODULE ...............................................................................	2-7
	2.1	Entry Module Raw .....................................................................................	2-7
	2.1.1	Design ......................................................................................................................	2-7
	2.1.2	Wiring Diagram Entry Module Raw: .............................................................	2-9
	2.1.3	Functional Description Entry Module .........................................................	2-10
	2.1.4	Calibration, adjusting ........................................................................................	2-11
	2.1.5	Removing Entry Module ..................................................................................	2-11
	2.1.6	Removing Assemblies ....................................................................................	2-12
	2.1.6.1	Entry lift ................................................................................................................	2-14
	2.1.6.2	Inc. tower - amb. temp., contr. temp. & Inc. tower lift ...........................	2-16
	2.1.6.3	Mains power unit ...............................................................................................	2-20
	2.1.7	Replacing spare parts .....................................................................................	2-21
	2.1.7.1	Cables E1, E2, E7, and E8 ............................................................................	2-21
	2.1.7.2	Cables E4, E5 and E6 .....................................................................................	2-22
	2.1.7.3	Cable E20 ............................................................................................................	2-23
	2.1.7.4	Connector board E11 & cable E22 .............................................................	2-24
	2.1.8	Reader complete ..............................................................................................	2-27
	2.1.8.1	Functional Description ....................................................................................	2-27
	2.1.8.2	Wiring Diagram Reader Complete .............................................................	2-27
	2.1.8.3	Replacing reader complete ...........................................................................	2-28
	2.1.9	Spare Parts List .................................................................................................	2-29
	2.1.10	3D Drawings .......................................................................................................	2-30
	2.1.11	Troubleshooting .................................................................................................	2-33

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2.2	Entry Module Frame ............................................................................	2-39
2.2.1	Functional Description ....................................................................................	2-39
2.2.2	Connector boards .............................................................................................	2-40
2.2.3	MTP (Micro Titer Plate)-Sensor (block diagram)....................................	2-46
2.2.4	Module Transport Assembly (wiring diagram).........................................	2-46
2.2.5	Replacing spare parts .....................................................................................	2-49
2.2.5.1	Connector board E1 ........................................................................................	2-49
2.2.5.2	Connector board E5 ........................................................................................	2-50
2.2.5.3	Connector board E2 ........................................................................................	2-50
2.2.5.4	Temperature sensor, tower fan (front) ......................................................	2-51
2.2.5.5	Tower fan (rear) .................................................................................................	2-52
2.2.5.6	Connector boards E6 and E7 .......................................................................	2-53
2.2.5.7	Connector board E13 ......................................................................................	2-54
2.2.5.8	Step motor, and gear belt ..............................................................................	2-55
2.2.5.9	Gear belt ..............................................................................................................	2-56
2.2.5.10	Connector board E10 ......................................................................................	2-58
2.2.5.11	Connector board E9 ........................................................................................	2-58
2.2.5.12	Cable E9 ..............................................................................................................	2-59
2.2.6	Spare Parts List .................................................................................................	2-60
2.2.7	3D Drawing Entry Frame ...............................................................................	2-63
2.2.8	Troubleshooting .................................................................................................	2-65

2.3	Entry Lift .........................................................................................................	2-73
2.3.1	Functional Description ....................................................................................	2-73
2.3.2	Connector Boards .............................................................................................	2-74
2.3.3	Wiring Diagram ..................................................................................................	2-76
2.3.4	Replacing Spare Parts ....................................................................................	2-77
2.3.4.1	Range flag ...........................................................................................................	2-77
2.3.4.2	Connector board E14 ......................................................................................	2-78
2.3.4.3	Step motor, and belt ........................................................................................	2-79
2.3.4.4	Thrust bearing and thrust washer ...............................................................	2-80
2.3.4.5	Lift nut ...................................................................................................................	2-80
2.3.4.6	Tension spring ....................................................................................................	2-82
2.3.5	Spare Parts List .................................................................................................	2-83
2.3.6	3D Drawing Entry Lift ......................................................................................	2-85
2.3.7	Troubleshooting .................................................................................................	2-87

2.3	Incubator Towers ....................................................................................	2-93
2.3.1	Incubator Tower Ambient Temp. ..................................................................	2-93
2.3.1.1	Functional Description ....................................................................................	2-93
2.3.1.2	Block Diagram ....................................................................................................	2-93
2.3.1.3	Connector Board ...............................................................................................	2-93

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2.3.1.4	Wiring Diagram ..................................................................................................	2-93
2.3.1.5	3D Drawing .........................................................................................................	2-93
2.3.1.6	Replacing Spare Parts ....................................................................................	2-93
2.3.1.7	Spare Parts List .................................................................................................	2-94
2.3.2	Incubator Tower Controlled Temp. .............................................................	2-95
2.3.2.1	Functional Description ....................................................................................	2-95
2.3.2.2	Connector Board ...............................................................................................	2-96
2.3.2.3	Wiring Diagram ..................................................................................................	2-98
2.3.2.4	Replacing Spare Parts .................................................................................	2-101
2.3.2.4.1	How to remove an incubation slot ...........................................................	2-101
2.3.2.4.2	Over temperature switch .............................................................................	2-102
2.3.2.4.3	Cable E22, temperature sensor ...............................................................	2-103
2.3.2.4.4	Heating element .............................................................................................	2-104
2.3.2.5	Spare Parts List ..............................................................................................	2-105
2.3.3	3D Drawing Incubator Tower Controlled ................................................	2-106
2.3.4	Troubleshooting ..............................................................................................	2-107
2.5	Incubator Tower Lift ...........................................................................	2-117
2.5.1	Functional Description ..................................................................................	2-117
2.5.2	Connector Boards ...........................................................................................	2-118
2.5.3	Wiring Diagram ................................................................................................	2-119
2.5.4	Replacing Spare Parts .................................................................................	2-120
2.5.4.1	Stepper motor E SM20 and / or gear belt .............................................	2-120
2.5.4.2	Thrust bearing, thrust washer ...................................................................	2-121
2.5.4.3	Connector board E12 ...................................................................................	2-122
2.5.4.4	Flag .....................................................................................................................	2-123
2.5.5	Spare Parts List ..............................................................................................	2-124
2.5.6	3D Drawing Incubator Tower Lift ..............................................................	2-126
2.5.7	Troubleshooting ..............................................................................................	2-127
2.6	Mains Power Unit .................................................................................	2-133
2.6.1	Functional Description .................................................................................	2-133
2.6.2	Connector Board ............................................................................................	2-134
2.6.3	Wiring Diagram ...............................................................................................	2-135
2.6.4	Replacing Spare Parts .................................................................................	2-136
2.6.5	Spare Parts List ..............................................................................................	2-136
2.6.6	3D Drawing Mains Power Unit ..................................................................	2-138
2.7	Entry Module Electronic Rack ..................................................	2-143
2.7.1	Motherboard E PCB .....................................................................................	2-144
2.7.2	Power Supply 1 PCB ....................................................................................	2-145
2.7.3	Processors PCB 1, PCB 2, PCB 3 ..........................................................	2-151
2.7.4	Stepper Motor PCB .......................................................................................	2-159

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	2.7.5	Entry PCB .........................................................................................................	2-165
	2.7.6	Incubator PCB ................................................................................................	2-171
Chapter	3	INCUBATOR MODULE ..............................................................	3-7
	3.1	Incubator Module Raw ..........................................................................	3-7
	3.1.1	Design ......................................................................................................................	3-7
	3.1.2	Wiring Diagram Inc. Module Raw ..................................................................	3-9
	3.1.3	Functional Description Incubator Module ................................................	3-10
	3.1.4	Calibration, Adjusting ......................................................................................	3-10
	3.1.5	Removing Incubator Module ..........................................................................	3-11
	3.1.6	Removing Assemblies .....................................................................................	3-11
	3.1.7	Replacing Spare Parts .....................................................................................	3-11
	3.1.8	Spare Parts List ..................................................................................................	3-11
	3.1.9	3D Drawings .......................................................................................................	3-12
	3.1.10	Troubleshooting .................................................................................................	3-15
	3.2	Incubator Module Frame ..................................................................	3-21
	3.2.1	Functional Description ....................................................................................	3-21
	3.2.2	Connector Boards .............................................................................................	3-22
	3.2.3	Replacing Spare Parts ....................................................................................	3-23
	3.2.4	Spare Parts List .................................................................................................	3-23
	3.2.5	3D Drawings .......................................................................................................	3-26
	3.2.6	Troubleshooting .................................................................................................	3-29
Chapter	4	WASHER / DISPENSER ...........................................................	4-7
	4.1	Washer / Dispenser Module Raw ................................................	4-7
	4.1.1	Design ......................................................................................................................	4-7
	4.1.2	Functional Description W / D Module ........................................................	4-10
	4.1.3	Removing Washer / Dispenser Module .....................................................	4-11
	4.1.4	Removing Assemblies ....................................................................................	4-12
	4.1.4.1	Dispenser ...........................................................................................................	4-13
	4.1.4.2	Reagent Drawer ................................................................................................	4-14
	4.1.4.3	Washer Lift ..........................................................................................................	4-16
	4.1.4.4	External pump station ....................................................................................	4-18
	4.1.5	Replacing Spare Parts ....................................................................................	4-19
	4.1.5.1	Motherboard W/D PCB ...................................................................................	4-19
	4.1.5.2	DC motor W PM 70 .........................................................................................	4-19
	4.1.5.3	AC motor W PM 80 ..........................................................................................	4-21
	4.1.5.4	Cable W32 ..........................................................................................................	4-21
	4.1.5.5	Water separator .................................................................................................	4-22

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4.1.8	Fluid-Flow Diagram .........................................................................................	4-27
4.1.9	Troubleshooting .................................................................................................	4-28
4.2	Washer / Dispenser Module Frame ........................................	4-35
4.2.1	Functional Description ....................................................................................	4-35
4.2.2	Connector Boards .............................................................................................	4-36
4.2.3	Wiring Diagram Washer Pumps ..................................................................	4-42
4.2.4	Replacing spare parts .....................................................................................	4-43
4.2.4.1	Connector board I1 PCB ................................................................................	4-43
4.2.4.2	Connector board W8 PCB .............................................................................	4-44
4.2.4.3	Connector board W11 PCB ..........................................................................	4-45
4.2.4.4	Connector board W13 PCB and seal ........................................................	4-46
4.2.4.5	Cable W50 ..........................................................................................................	4-47
4.2.4.6	Cable W43 ..........................................................................................................	4-48
4.2.4.7	Step motor W SE10 .........................................................................................	4-49
4.2.4.8	Gear belt open ...................................................................................................	4-50
4.2.4.9	Gear belt MXL ....................................................................................................	4-51
4.2.5	Spare Parts List .................................................................................................	4-53
4.2.6	3D Drawing Washer / Dispenser Frame ..................................................	4-55
4.2.7	Troubleshooting .................................................................................................	4-56
4.3	Dispenser ......................................................................................................	4-61
4.3.1	Functional Description ....................................................................................	4-61
4.3.2	Connector Boards .............................................................................................	4-63
4.3.3	Wiring Diagram ..................................................................................................	4-67
4.3.4	Replacing spare parts .....................................................................................	4-71
4.3.4.1	Connector board W9 PCB and cable W8 ................................................	4-71
4.3.4.2	Step motor W SE30 and Gear belt MXL ..................................................	4-74
4.3.4.3	Regulating screw 2 compl. ............................................................................	4-75
4.3.4.4	Guide bush (on guide shaft 3) ......................................................................	4-75
4.3.4.5	Guide bush (on guide shaft 5) ......................................................................	4-77
4.3.4.6	Step motor W SM25 and step motor W SE20 .......................................	4-78
4.3.4.7	Gear belt MXL and Gear belt .......................................................................	4-79
4.3.4.8	Gear belt ..............................................................................................................	4-81
4.3.4.9	Connector board W6 PCB .............................................................................	4-84
4.3.4.10	Regulating screw 1 compl. ............................................................................	4-86
4.3.4.11	Connector board W5 PCB .............................................................................	4-86
4.3.4.12	Cable W7 .............................................................................................................	4-87
4.3.5	Spare Parts List .................................................................................................	4-89
4.3.6	3D Drawings .......................................................................................................	4-92
4.3.7	Troubleshooting .................................................................................................	4-94

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4.4	Reagent Drawer ....................................................................................	4-103
4.4.1	Functional Description .................................................................................	4-103
4.4.2	Connector Boards ..........................................................................................	4-104
4.4.3	Wiring Diagram ................................................................................................	4-110
4.4.4	Replacing spare parts ...................................................................................	4-113
4.4.4.1	Connector board W1 PCB ...........................................................................	4-113
4.4.4.2	Reagent carousel support ...........................................................................	4-114
4.4.4.3	Contact spring ..................................................................................................	4-115
4.4.4.4	Step motor W SE40, Gear belts ................................................................	4-116
4.4.4.5	Connector board W4 PCB ...........................................................................	4-117
4.4.4.6	Barcode reader BR45 ...................................................................................	4-118
4.4.4.7	Connector board W2 PCB ...........................................................................	4-119
4.4.4.8	Hall effect micro switch drawer access Cable W14 ..........................	4-120

4.4.4.9Hall effect micro switch drawer end position

	Cable W15 ........................................................................................................	4-120
4.4.4.10	Cable W12 .......................................................................................................	4-121
4.4.4.11	Drawer compartment ....................................................................................	4-121
4.4.5	Spare Parts List ..............................................................................................	4-122
4.4.6	3D Drawing Reagent Drawer ....................................................................	4-124
4.4.7	Troubleshooting ..............................................................................................	4-125
4.5	Washer Lift ................................................................................................	4-133
4.5.1	Functional Description .................................................................................	4-133
4.5.2	Connector Boards ..........................................................................................	4-134
4.5.3	Wiring Diagram ...............................................................................................	4-141
4.5.4	Replacing spare parts ..................................................................................	4-145
4.5.4.1	Step motor W SM50, Gear belts ..............................................................	4-145
	Gear belts: ........................................................................................................	4-146
4.5.4.2	Manifold coupling compl. ............................................................................	4-148
4.5.4.3	Shell spring ......................................................................................................	4-150
4.5.4.4	Wash shell ........................................................................................................	4-152
4.5.4.5	Connector board W12 PCB .......................................................................	4-152
4.5.4.6	Connector board W14 PCB .......................................................................	4-153
4.5.4.7	Connector board W16 PCB .......................................................................	4-154
4.5.4.8	Cable W44 ........................................................................................................	4-154
4.5.5	Manifold Complete ........................................................................................	4-155
4.5.6	Spare Parts List ..............................................................................................	4-156
4.5.7	3D Drawing Washer Lift ..............................................................................	4-158
4.5.8	Troubleshooting ..............................................................................................	4-159
4.6	External Pump Station ....................................................................	4-167
4.6.1	Functional Description .................................................................................	4-167

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	4.6.2	External Pump Station PCB .......................................................................	4-168
	4.6.3	Wiring Diagram External Pump Station .................................................	4-174
	4.6.4	Replacing spare parts ..................................................................................	4-175
	4.6.4.1	Removing and remounting of PCB cover .............................................	4-175
	4.6.4.2	Silicone Tubing ...............................................................................................	4-175
	4.6.4.3	DC motor W PM61 ........................................................................................	4-176
	4.6.4.4	External pump station PCB ........................................................................	4-176
	4.6.4.5	Wash fluid level sensor ...............................................................................	4-177
	4.6.4.6	Cable W61 .......................................................................................................	4-178
	4.6.4.7	O-ring .................................................................................................................	4-178
	4.6.4.8	O-Rings, Float, Compression Springs ...................................................	4-179
	4.6.4.9	EPROM .............................................................................................................	4-180
	4.6.5	Spare Parts List ..............................................................................................	4-181
	4.6.6	3D Drawing External Pump Station ........................................................	4-183
	4.6.7	Troubleshooting ..............................................................................................	4-184
	4.7	W/D Electronic Rack .........................................................................	4-191
	4.7.1	Motherboard W / D PCB .............................................................................	4-192
	4.7.2	Power Supply 2 PCB ....................................................................................	4-193
	4.7.3	Processor 2 PCB ...........................................................................................	4-194
	4.7.4	Processor 3 PCB ...........................................................................................	4-194
	4.7.5	Stepper Motor PCB .......................................................................................	4-194
	4.7.6	Dispenser PCB ...............................................................................................	4-195
	4.7.7	Washer PCB ...................................................................................................	4-201
Chapter	5	END MODULE ........................................................................................	5-7
	5.1	End Module Raw ........................................................................................	5-7
	5.1.1	Design ......................................................................................................................	5-7
	5.1.2	Functional Description End Module ..............................................................	5-9
	5.1.3	Calibration, Adjusting .........................................................................................	5-9
	5.1.4	Removing End Module ...................................................................................	5-10
	5.1.5	Removing Assemblies .....................................................................................	5-11
	5.1.5.1	Dispenser ............................................................................................................	5-12
	5.1.5.2	Reagent drawer .................................................................................................	5-13
	5.1.5.3	Build-in Photometer .........................................................................................	5-13
	5.1.5.4	Exit Stack .............................................................................................................	5-13
	5.1.6	Replacing Spare Parts ....................................................................................	5-14
	5.1.6.1	Pause Button ......................................................................................................	5-14
	5.1.7	Spare Parts List .................................................................................................	5-15
	5.1.8	3D Drawings End Module Raw ....................................................................	5-16

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5.1.9	Troubleshooting End Raw/ End Dispenser/ End Drawer ...................	5-19
5.2	End Module Frame ................................................................................	5-31
5.2.1	Functional Description ....................................................................................	5-31
5.2.2	Connector Boards .............................................................................................	5-33
5.2.3	Wiring Diagram ..................................................................................................	5-37
5.2.4	Replacing spare parts .....................................................................................	5-41
5.2.4.1	Connector board P1 PCB ..............................................................................	5-41
5.2.4.2	Connector board W8 PCB .............................................................................	5-41
5.2.4.3	Connector board P7 PCB ..............................................................................	5-42
5.2.4.4	Connector board P8 PCB ..............................................................................	5-42
5.2.4.5	Step motor P SE01 ..........................................................................................	5-43
5.2.4.6	Step motor W SE10 .........................................................................................	5-43
5.2.4.7	Gear belt ..............................................................................................................	5-44
5.2.4.8	Gear belt (module transport) ........................................................................	5-45
5.2.4.9	Gear belt (main shuttle drive) .......................................................................	5-46
5.2.5	Spare Parts List .................................................................................................	5-49
5.2.6	3D Drawing End Module Frame ..................................................................	5-51
5.2.7	Troubleshooting .................................................................................................	5-52
5.3	Built-in Photometer ..............................................................................	5-59
5.3.1	Functional Description ....................................................................................	5-59
5.3.2	Connector Boards .............................................................................................	5-60
5.3.3	Wiring Diagram ..................................................................................................	5-60
5.3.4	Replacing Spare Parts ....................................................................................	5-63
5.3.4.1	Filter case, filter strip .......................................................................................	5-63
5.3.4.2	Photometer processor PCB ..........................................................................	5-64
5.3.4.3	Lamp controller PCB .......................................................................................	5-64
5.3.4.4	Lamp-assembly PCB .......................................................................................	5-65
5.3.4.5	Photometer fan ..................................................................................................	5-66
5.3.4.6	Cables P20, P21, P23 and P24 .................................................................	5-67
5.3.4.7	Optical device .....................................................................................................	5-68
5.3.4.8	Connector board P20 .....................................................................................	5-70
5.3.4.9	Photometer air filter ..........................................................................................	5-70
5.3.4.10	Step motor ...........................................................................................................	5-71
5.3.4.11	Filter case belt ...................................................................................................	5-73
5.3.4.12	A/D converter PCB ...........................................................................................	5-75
5.3.4.13	Protective glass .................................................................................................	5-77
5.3.5	Spare Parts List .................................................................................................	5-79
5.3.6	Photometer Filter Order List: ........................................................................	5-79
5.3.7	Troubleshooting .................................................................................................	5-80

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5.4	Exit Stack ......................................................................................................	5-87
5.4.1	Functional Description ....................................................................................	5-87
5.4.3	Wiring Diagram ..................................................................................................	5-96
5.4.4	Replacing Spare Parts ....................................................................................	5-99
5.4.4.1	Connector board P2 ........................................................................................	5-99
5.4.4.2	Connector board P3 .....................................................................................	5-100
5.4.4.3	Connector board P4 .....................................................................................	5-101
5.4.4.4	Connector board P5 .....................................................................................	5-102
5.4.4.5	Connector board P6 .....................................................................................	5-103
5.4.4.6	Transparent cover sensor ..........................................................................	5-103
5.4.4.7	Step motor, gear belt ....................................................................................	5-105
5.4.4.8	Connector board P9 .....................................................................................	5-106
5.4.4.9	Gear belt ...........................................................................................................	5-107
5.4.5	Spare Parts List ..............................................................................................	5-108
5.4.6	3D Drawing Exit Stack ..................................................................................	5-110
5.4.7	Troubleshooting ...............................................................................................	5-111

	5.5	Electronic Rack ......................................................................................	5-118
	5.5.1	Motherboard P PCB ......................................................................................	5-119
	5.5.2	Power Supply 2 PCB ....................................................................................	5-120
	5.5.3	Processor 1 PCB ...........................................................................................	5-120
	5.5.4	Processor 2 PCB ...........................................................................................	5-120
	5.5.5	Processor 3 PCB ...........................................................................................	5-120
	5.5.6	Stepper Motor PCB .......................................................................................	5-120
	5.5.7	Dispenser PCB ...............................................................................................	5-120
	5.5.8	Exit PCB ............................................................................................................	5-121
Chapter	6	INSTRUMENT FRAMEWORK ..........................................	6-7
	6.1	Instrument Error Codes .......................................................................	6-7
	6.1.1	Entry System .........................................................................................................	6-7
	6.1.2	Incubator System .................................................................................................	6-9
	6.1.3	Dispenser System .............................................................................................	6-11
	6.1.4	Washer System .................................................................................................	6-13
	6.1.5	Exit System .........................................................................................................	6-15
	6.1.6	Communication System .................................................................................	6-16
	6.1.7	Photometer System .........................................................................................	6-17
	6.2	Container Stack ........................................................................................	6-23
	6.3	Chassis ............................................................................................................	6-31
	6.4	Main Shuttle Rail ...................................................................................	6-45
	6.5	Transparent Cover .................................................................................	6-55
	6.6	Waste Cap .....................................................................................................	6-63

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CHAPTER 1 SYSTEM

1.1ML F.A.M.E. Instrument

1.1.1General Information

Refer to User Manual Chapter 1.

ATTENTION

Read Chapter 1, General Instructions, of the User Manual before repairing / servicing the system.

For specific Service Information read section 1.1.2 Service Concept.

ATTENTION

Never lift a fully installed (assembled) instrument from one work bench to another. It must first be dismantled and then reinstalled on the new work bench.

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Location of Warning and Attention Labels

Attention: Do not swap plates after loading

Power

Serial No.

CE Label

PAUSE

(Pause Button)

Warning: Laser Beam

Do Not Stare Into Beam!	Warning:	BIOHAZARD
Class 2 Laser

Attention: Shut down OS/2 before switching off PC.

Do not change the OS/2 system time during a run.

Warning:		Moving Parts

Attention: Turn carousel until it drops into locked position. Ensure all plungers are pushed down and caps are properly closed.

Before removing the module decontaminate the instrument, unplug all the connectors and seal the fluid system as described in the manual.

Attention: Close the reagent drawer gently to prevent spillage. Do not fill containers above the “max. 100 ml” mark.

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Power Connection:

Connect only to earth grounded outlet

115V~/...A 60Hz

230V~/...A 50Hz

The pause button should generally not be pressed unless instructed by the user software as plate processing may be interrupted leading to aborted plate. Carefully follow the software instructions.

Warning Symbols

General	Danger
Warning	High Voltage

	BIOHAZARD	Danger
		Laser
	HANDLE WITH CARE

Command Symbols

General	Wear
Command	Gloves

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1.1.2Service Concept

1.1.2.1General Information

This Service Manual is a field service guide and is to be used only by Service Technicians trained and/or authorized by Hamilton Bonaduz AG.

Repaired units must meet the quality standards set by Hamilton Bonaduz AG.

No part of this manual may be copied or handed on to a third party. Owners of Service Manuals are registered and only they will be issued with update information such as Service Manual Updates and Service Bulletins.

ATTENTION

In order to prevent infections caused by an unclean instrument in clinical environments (blood banks etc.), contaminated parts to be touched while repairing should be cleaned and sterilised - a precautionary measure of greatest importance.

Only after cleaning and disinfecting should repairs be carried out.

In order to protect the Service Personnel from contagion, protective vaccination is advised.

1.1.2.2ML F.A.M.E. Service Manual

The ML F.A.M.E. Service Manual is designed to visually guide the service technician to the appropriate section as quickly as possible. It consists of 6 chapters:

1SYSTEM

2ENTRY MODULE

3INCUBATOR MODULE

4WASHER / DISPENSER

5END MODULE

6INSTRUMENT FRAMEWORK

Chapter 1 serves as an introduction to the ML F.A.M.E. instrument and the ML F.A.M.E. manual. Service technicians must read chapter 1 in order to provide the best service.

Chapters 2-6 contain the servicing instructions.

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1.1.2.2.1Description of text icons

Attentions and useful notes are included in this manual to emphasize important and critical instructions. They are accompanied by a special symbol in the margin of the page and are printed in italics.

ATTENTION

All special problems, warnings or important text will be accompanied by this symbol at the appropriate position.

NOTE

Text associated with this symbol provides the user with useful notes. Carefully read the text as it is important for understanding the specific topic/command.

1.1.2.2.2Updating the Service Manual

As the instrument or parts of the instrument are constantly being improved, this Service Manual will regularly be updated. Each Service Technician will be sent update-sets and is responsible that his Service Manual is kept up to date. Necessary instructions of how to do this will be included in the update-set.

It is most important that your Service Manual is kept up to date. On receipt of an Update, carefully follow the instructions on the cover page and then file the cover page after this section.

1.1.2.2.3Service Bulletins

Service Bulletins are sent to immediately inform the Service Technician. File the Bulletins in this section to keep the Manual up to date.

1.1.2.2.4Service Part Classifications

	Spare Part	Description
	Class (SPC)
	I	Instrument
	A	Service Technician always has these parts with him
	B	Dealer has part in stock
	C	Hamilton has part in stock
	P	Promotion Material
	T	Service Tools
	V	Consumables
	Z	Accessories
		Not a Spare Part

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1.1.2.2.5Service Assemblies

Parts labelled with part numbers rather than item numbers on the 3D drawings are Service Assemblies and should not be disassembled by the Service Technician. If a part of the assembly is defective, the whole assembly must be replaced.

1.1.2.2.6ML F.A.M.E. instruments with CE conformity

Instruments with serial numbers greater than 2000 are CE conform. Below is a table listing which board revisions may be installed into CE conform instruments.

	Description	Part Number	New Revisions
			CE Conform
	Motherboard E PCB	146 164	01
	Motherboard W/D PCB	146 168	01
	Motherboard P PCB	146 170	01
	Incubator PCB	146 178	03
	Entry PCB	146 176	03
	Exit PCB	146 186	03
	Power Supply 2 PCB	146 172	04
	Power Supply 1 PCB	146 284	04
	External Pump Station PCB	146 240	02

ATTENTION

The following boards (including those with a lower revision) must not be used in CE-conform instruments: Incubator PCB 146178/02, Exit PCB 146186/02, Power Supply 2 PCB 146172/03, and External Pump Station PCB 146240/01.

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1.1.2.2.7Pin coding for PCBs

• = Pin fitted

Type: Codes for Processor Boards:

Codes for Motherboards:

A

B

C

D

E

F

G

H

I

K

L

M

A

B

C

D

E

F

G

H

I

K

L

M

•

UP 1

•

•

•

•

•

•

•

•

•

•

•

UP 2

•

•

•

•

•

•

•

• •

•

•

•

UP 3

•

•

•

•

•

•

•

• •

•

•

•

UP 4

•

•

•

•

•

•

•

•

•

•

Codes for Peripheral Boards:

A

B

C

D

E

F

G

H

I

K

L

M

A

B

C

D

E

F

G

H

I

K

L

M

•

Power Supply 1 PCB

•

•

•

•

•

•

•

•

•

•

•

Power Supply 2 PCB

•

•

•

•

•

•

•

•

• •

•

•

Entry

•

•

•

•

•

•

•

•

•

•

•

Incubator

•

•

• •

•

•

•

•

•

•

•

•

Step Motor

•

•

• •

•

•

•

• •

•

•

•

Dispenser

•

•

• •

•

•

•

• • •

•

•

Washer

•

•

• •

•

•

•

• • •

•

•

Exit

•

•

•

•

•

•

•

•

•

•

•

1.1.2.2.8Connector numbering

The drawing on the following page 614 149 illustrates the connector numbering system used for ML F.A.M.E.

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Wiring Principle Block Diagram 614 149

revision 00

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1.1.2.3Feedback (Quarterly Service Call Statistics)

Information from the field is a determining factor for improving Hamilton products. Please send/fax a copy of all of your Service Reports to Hamilton Bonaduz AG every three months so that we may continue to provide quality products and services.

1.1.2.4Parts Return Tag

The part Return Tag has two functions:

Page 1: Part identification, reason for return and description of problem

Page 2: Decontamination Declaration

Material Return Good Authorization

Parts or instruments may only be returned to Hamilton Bonaduz AG with a Return Good Authorization (RGA).

Ask the Hamilton Order Processing Department (OPD) for an RGA number prior to sending any material. This number must be entered in the appropriate blank on the Parts Return Tag.

A completed Part Return Tag must be attached to all parts or instruments which are returned to HAMILTON BONADUZ AG. Tags may be ordered from Hamilton Bonaduz AG (p/n 612 554).

Hamilton Bonaduz AG reserves the right to return any parts or instruments which may pose a health hazard due to contamination and to charge the customer for any expenses incurred.

1.1.2.5Technical Data Sheet

The technical data sheet is supplied with each instrument and contains all technical data of a specific instrument. It must be updated by the service technician when significant modifications are made.

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1.1.2.6Service Kit

1.1.2.6.1Service Kit Spare Parts List 148 236

SPARE CASE ML F.A.M.E. REV: 00

	P/N	DESCRIPTION	REVISION	ITEM	QUANTITY PER	SPARE PART
				NUMBER	ASSEMBLY	CLASS
	141239	KLEMMRING	01	1	1.000	A
	146154	CONNECTORBOARD E1 PCB	00	2	1.000	A
	146156	CONNECTORBOARD I1 PCB	00	3	1.000	A
	146158	CONNECTORBOARD P1 PCB	00	4	1.000	A
	146172	POWER SUPPLY 2 PCB	04	7	1.000	A
	146174	STEP MOTOR PCB	00	8	1.000	A
	146178	INKUBATOR PCB	02	9	1.000	A
	146180	DISPENSER PCB	01	10	1.000	A
	146182	WASHER PCB	02	11	1.000	A
	146186	EXIT PCB	03	12	1.000	A
	146188	IC27C256 PRO VEDLS10.ACH	00	13	1.000	A
	146195	CONNECTORBOARD W1 PCB	02	14	1.000	A
	146197	CONNECTORBOARD W2 PCB	02	15	1.000	A
	146201	CONNECTORBOARD W4 PCB	00	16	1.000	A
	146407	CONNECTORBOARD W5 PCB	00	17	1.000	A
	146209	CONNECTORBOARD W8 PCB	00	19	1.000	A
	146411	CABLE W7	00	20	1.000	A
	146218	CABLE W8	00	21	1.000	A
	146222	CABLE W12	00	22	1.000	A
	146230	CONNECTORBOARD W11 PCB	00	23	1.000	A
	146232	CONNECTORBOARD W12 PCB	00	24	1.000	A
	146234	CONNECTORBOARD W13 PCB	00	25	1.000	A
	146236	CONNECTORBOARD W14 PCB	00	26	1.000	A
	146240	EXTERNAL PUMP STATION PCB	02	27	1.000	A
	146243	PHOTOMETER PROCESSOR PCB	00	29	1.000	A
	146244	CONNECTORBOARD P20 PCB	00	30	1.000	A
	146245	LAMP CONTROLLER PCB	00	31	1.000	A
	146256	CONNECTORBOARD W9 PCB	01	32	1.000	A
	146257	CONNECTORBOARD E8 PCB	00	33	1.000	A
	146259	CONNECTORBOARD P2 PCB	00	34	1.000	A
	146261	CONNECTORBOARD P3 PCB	00	35	1.000	A
	146263	CONNECTORBOARD P4 PCB	00	36	1.000	A
	146265	CONNECTORBOARD P5 PCB	00	37	1.000	A
	146266	CONNECTORBOARD P6 PCB	00	38	1.000	A
	146268	CONNECTORBOARD P9 PCB	00	39	1.000	A
	146271	CONNECTORBOARD P8 PCB	00	40	1.000	A
	146275	LAMP-ASSEMBLY PCB	00	41	1.000	A
	146278	CONNECTORBOARD W16 PCB	01	42	1.000	A
	146298	CABLE W44	00	43	1.000	A
	146308	TEMPERATURE SENSOR TS70	00	44	1.000	A
	146311	CONNECTORBOARD E6 PCB	00	45	1.000	A
	146312	CONNECTORBOARD E7 PCB	00	46	1.000	A
	146313	CONNECTORBOARD E9 PCB	00	47	1.000	A

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	P/N	DESCRIPTION	REVISION	ITEM	QUANTITY PER	SPARE PART
				NUMBER	ASSEMBLY	CLASS
	146314	CONNECTORBOARD E10 PCB	00	48	1.000	A
	146322	CONNECTORBOARD E11 PCB	00	49	1.000	A
	146352	CABLE E20	00	50	1.000	A
	146384	A/D CONVERTER PCB	00	53	1.000	A
	146386	CONNECTORBOARD E14 PCB	00	54	1.000	A
	146388	CONNECTORBOARD E12 PCB	00	55	1.000	A
	146390	CONNECTORBOARD E13 PCB	00	56	1.000	A
	147417	ISOLIERSCHEIBE	00	58	1.000	A
	147489	SCHWIMMER	01	59	1.000	A
	147638	KABELKLEMME	00	61	1.000	A
	147683	FILTERMATTE	00	62	1.000	A
	147693	VERBINDUNGSSTUECK NW 8/45?	00	63	1.000	A
	147720	SHELL-FEDER	00	64	1.000	A
	147777	ABDECKFOLIE	00	65	1.000	A
	147935	MTP-TRANSPORTER	00	66	1.000	Z
	148157	KONTAKTFEDER	00	67	1.000	A
	148228	MODULE TRANSPORT FORK COMPL.	01	68	1.000	A
	148230	REGULATING SCREW 1 COMPL.	00	69	1.000	A
	148231	REGULATING SCREW 2 COMPL.	00	70	1.000	A
	249944	SCHLAUCHVERBINDER 6/10/6	00	71	1.000	A
	254101	O-RING 85.32x3.53 VITON 75	00	72	1.000	A
	254106	O-RING 12.00X2.00 VITON	00	73	1.000	A
	254110	O-RING 76.00X2.00 VITON	00	74	1.000	A
	254111	O-RING 5.00X1.50 VITON	00	75	1.000	A
	254121	O-RING 79X2 NBR 70.5/P5	00	76	1.000	A
	256115	ZUGFED. D 0,32 DA 3,52 LO 12,9	00	77	1.000	A
	256116	ZUGFEDER D 0.50 DM 3.0 LO 24.5	00	78	1.000	A
	257036	DISTANZHALTER M3 X 8 I/I GEW.	00	79	1.000	A
	257040	DIST.HALTER M4X20 I/A GEW.	00	80	1.000	A
	257041	DIST.HALTER M4X 25 I/A GEW.	00	81	1.000	A
	279448	SCHWINGUNGSDAEMPFER D=15 H=20		83	1.000	A
	279648	WINKELSTUECK 90? PP ID 4-5	00	84	1.000	A
	279649	WINKELSTUECK 90? PP ID 8-9	00	85	1.000	A
	361019	KABELBEF. SES-CT	00	86	3.000
	422002	SPREIZNIETE KST D=5 KB=3.5-4.5	00	90	1.000	A
	420214	PT-SENKKOPFSCHRAUBE KA 30 X 8	00	91	2.000	A
	7279355	KLEBBAND 1-SEITIG D= 2 B= 6	00	92	0.200
	147963	PHOTOMETER AIR FILTER	00	93	1.000	A
	511006	LOCTITE 222 50mL	00	94	1.000	T
	511010	LOCTITE 638 50mL	00	96	1.000	T
	7249057	SILIKONSCHL 4 X 7 TRANSP	00	97	1.000	B
	7260010	SILIKONSCHL 8 X12 TRANSP	00	98	1.000	B
	148248	KLETTVERSCHLUSS	00	99	4.000	A
	7281149	PTFE-KLEBEBAND EXTR.B=9MM	00	100	0.200
	7281122	KLEBBAND 2-SEITIG D=0,8 B=9	00	101	0.200
	146328	TRANSPARENT COVER SENSOR HS03	00	102	1.000	A
	146416	REAGENT DRAWER SWITCH SET	00	103	1.000	A
	254105	O-RING 8.00X2.00 VITON	00	104	1.000	A
	254126	O-RING Di 5x2.5 VITON 75	00	105	1.000	A
	281110	STOPFEN D5.8 D7 L5 SILIKON	00	106	1.000	A

1-11

——— ML F.A.M.E. Service Manual V2.0 ———————————————————————————————————————————— SYSTEM ———

	P/N	DESCRIPTION	REVISION	ITEM	QUANTITY PER	SPARE PART
				NUMBER	ASSEMBLY	CLASS
	146405	IC27C256 PRO VEEPS11.ACH	00	107	1.000	A
	235416	FARBFL. 7,5ML HELLBLAU HAM	00	108	1.000	T
	235425	FARBFL. 7,5ML GRAUBLAU HAM	00	109	1.000	T
	235418	HAERTERFLASCHE 7,5 ML	00	110	1.000	T
	235417	FARBFL. 7,5ML DUNKELBLAU HAM	00	111	1.000	T
	147581	JUSTIERSCHEIBE	00	112	2.000	A
	369051	VERPACK CHIP CONTAINER TYP 2	00	114	1.000
	511057	OEL MICROGLISS D-5 20ML	00	115	1.000	T
	146394	CABLE MODULE CONNECTION	00	116	2.000	T
	146395	SERVICE PCB	00	117	1.000	T
	239038	ELEKTRONIKKOFFER	00	118	2.000
	239039	EINLEGEPLATTE	00	119	8.000
	148245	SETSCREW	00	120	1.000	A
	147516	WASH FLUID LEVEL SENSOR	00	121	1.000	A
	361036	KABELHALTER	00	122	3.000
	361002	KABELBEF. 375 S	00	123	3.000
	361004	KABELBEF. CFCC-4 1/2”	00	124	3.000
	369038	VERRIEGELUNGSMUTTER	00	125	1.000	B
	255038	KENNZEICHNUNG LABEL 21X9 WEISS	00	126	2.000
	148339	SUPPORT ENTRY MODULE	00	128	1.000	T
	148355	TRANSP COVER MAGNET	00	129	1.000	T
	146409	CONNECTORBOARD W6 PCB	00	130	1.000	A
	622054	PACKING LIST ML FAME	05	131	1.000
	511069	MOLYKOTE FETTPASTE DX	00	132	1.000	T
	146317	PROCESSOR 4 PCB	01	135	1.000	A
	235426	FARBFL.7,5ML SIGNALSCHW.	00	136	1.000	T
	235427	FARBFL.7,5ML ACHATGRAU	00	137	1.000	T

1-12

——— ML F.A.M.E. Service Manual V2.0 ————————————————————————————————————————————— SYSTEM ———

1.1.2.6.2Service PCB (to be used with Diagnostic Master)

Component Layout 146.395 revision 00

1-13

——— ML F.A.M.E. Service Manual V2.0 ———————————————————————————————————————————— SYSTEM ———

Service PCB

Wiring Diagram

604.852

revision 00

GND 5V

GND 5V

GND 5V +5VDC +5VDC +5VDC

+35VDC on WA

VACUUM PUMP on WA

STEP MOTOR on SM / VACUUM PUMP on WA

STEP MOTOR on SM, WA / +35VDC on IN

STEP MOTOR on SM, WA /HEATING ELEMENT on IN

STEP MOTOR on SM, DS, WA / HEATING ELEMENT on IN

STEP MOTOR on SM, DS, WA, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX / HEATING ELEMENT on IN

STEP MOTOR on SM, ET, EX / HEATING ELEMENT on IN +5VDC +5VDC +5VDC GND 5V GND 5V GND 5V

NOT MOUNTED

1a

1b

1c

2a

2b

2c

3a

3b

3c

4a

4b

4c

5a

5b

5c

6a

6b

6c

7a

7b

7c

8a

8b

8c

9a

9b

9c

10a

10b

10c

11a

11b

11c

12a

12b

12c

13a

13b

13c

14a

14b

14c

15a

15b

15c

16a

16b

16c

17a

17b

17c

18a

18b

18c

19a

19b

19c

20a

20b

20c

21a

21b

21c

22a

22b

22c

23a

23b

23c

24a

24b

24c

25a

25b

25c

26a

26b

26c

27a

27b

27c

28a

28b

28c

29a

29b

29c

30a

30b

30c

31a

31b

31c

32a

32b

32c

J1

HEATING ELEMENT on IN

STEP MOTOR on SM, ET, EX

TP16

HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX

TP15

HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX

TP14

HEATING ELEMENT on IN

STEP MOTOR on SM, ET, DS, EX

TP13

HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX

TP12

HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX

TP11

HEATING ELEMENT on IN

STEP MOTOR on SM, DS, EX

TP10

HEATING ELEMENT on IN

STEP MOTOR on SM, DS, WA, EX

TP9

HEATING ELEMENT on IN

STEP MOTOR on SM, DS, WA

TP8

HEATING ELEMENT on IN

STEP MOTOR on SM, WA

TP7

+35VDC on IN

STEP MOTOR on SM, WA

TP6

VACUUM PUMP on WA

STEP MOTOR on SM

TP5

VACUUM PUMP on WA

TP4

+35VDC on WA

TP3

+5VDC

TP2

GND_5V

TP1

P1

10a

11a

11b

12b

14a

27b

32b

1a

1b

1c

2a

2b

2c

3a

3b

3c

4a

4b

4c

5a

5b

5c

6a

6b

6c

7a

7b

7c

8a

8b

8c

9a

9b

9c

10b

10c

11c

12a

12c

13a

13b

13c

14b

14c

15a

15b

15c

16a

16b

16c

17a

17b

17c

18a

18b

18c

19a

19b

19c

20a

20b

20c

21a

21b

21c

22a

22b

22c

23a

23b

23c

24a

24b

24c

25a

25b

25c

26a

26b

26c

27a

27c

28a

28b

28c

29a

29b

29c

30a

30b

30c

31a

31b

31c

32a

32c

GND 5V

GND 5V GND 5V

+5VDC

+5VDC

+5VDC

+5VDC

+5VDC

+5VDC

GND 5V

GND 5V

GND 5V

SM => STEP MOTOR PCB IN => INCUBATOR PCB ET => ENTRY PCB DS => DISPENSER PCB WA => WASHER PCB EX => EXIT PCB

EL-RACK MOTHERBOARD E PCB/- W PCB/- P PCB

LEGEND:

1-14

——— ML F.A.M.E. Service Manual V2.0 ————————————————————————————————————————————— SYSTEM ———

1.1.3F.A.M.E. Description

Microlab F.A.M.E., a Fully Automated Microplate ELISA processor, carries out all necessary steps of a microplate ELISA after sample distribution with minimum user interference: plate incubation, plate washing, reagent dispensing and photometric evaluation.

With modular design of the different functions, the instrument can be adjusted to individual laboratory requirements. The standard configuration contains enough plate incubation and reagent capacity to ensure a throughput of approximately 10 microplates/hour with continuous loading capability. Bar codes on plates and reagents ensure positive identification throughout the process. Zero carry-over is achieved using disposable reagent containers and syringes.

The instrument is driven by a user-friendly software for definition, processing and interpretation of the vast majority of available ELISA tests.

Modular, extendable design, can be adapted to the various laboratory needs.

Modular design allows parallel processing of ELISA steps.

Automation

The standard configuration comprises enough plate incubation (20 slots) and reagent capacity (16 containers), to ensure high throughput.

Walk-away

Continuous processing: ML F.A.M.E. may be continuously loaded with micro titer plates (up to 4) which are processed as long as there is capacity available. A separate exit stack, where up to ten micro titer plates can be stacked, keeps the entry stack free for loading plates.

Complete instrumentation

Microlab F.A.M.E. ensures positive identification via barcodes on plates and reagents.

No carry-over, using disposable syringes (for each reagent container a syringe).

No carry-over with the intelligent cleaning procedure and the autorinse of wash system.

Open System

Microlab F.A.M.E. is able to process tests of various manufacturers simultaneously.

Test kits can easily be adapted.

Method library, kit lot library, reagents library, wash solutions library.

Complete test evaluation (qualitative, quantitative, semi quantitative) and test reports.

Communication and LAN Integration

1-15

——— ML F.A.M.E. Service Manual V2.0 ———————————————————————————————————————————— SYSTEM ———

Communication with sample preparation instruments, e.g. ML AT / ATplus 2. Barcode data of pipetted samples and pipetting information are linked to the patient results. Results and protocols can be sent via external communication systems to a HOST computer.

1.1.3.1Purpose of the system

ML F.A.M.E. is an automated bench-top analyzer for immunological tests based on the ELISA (Enzyme Linked Immuno Sorbent Assay) test principle in a defined format, so-called micro titration plates (MTP). ML F.A.M.E. will carry out all necessary processing steps for an ELISA test, including data evaluation and interpretation; however, the primary sample preparation step (i.e. transferring the samples into the MTP wells) is not included in ML F.A.M.E. This step will be carried out with pipetting robots like the Hamilton Microlab AT, ATplus or the Microlab 2200.

An ELISA test usually includes several steps, thus the following features are included into ML F.A.M.E.:

incubation of the MTP at a defined temperature for a defined time

washing of the MTP, i.e. aspirating the liquid in the wells, filling it with wash fluid and emptying the wells

dispensing of reagents into the MTP wells

photometric measurement of the MTP wells

validation and interpretation of the photometric data and creation of result documentation.

The analyzer is designed as a walk-away device i.e. minimum user interaction during processing is required. Walk-away means that the operator is occupied with other tasks, but is able to respond fast if necessary.

ML F.A.M.E. is controlled by a computer system running the OS/2 operating system. DB/2 is used for the storage of data. The computer is not a built-in device and the customer is expected to provide it at his/her cost. A list of compatible computer types will be provided by Hamilton.

1.1.3.2General Operation Procedure

Test plates with serum, controls and standards pipetted into the wells either manually or by using a sample pipetting instrument, are loaded onto the entry position of ML F.A.M.E. Plates can be introduced either individually or in stacks of up to four MTPs. The barcoded plate is identified by the ML F.A.M.E. bar code reader (with manual identification also possible) and then processed according to the method to which the plate barcode has been assigned. After the last processing step, the MTP is automatically placed on the exit stack, which can hold up to 10 MTPs. Also, photometric data evaluation and test interpretation is started once the O.D. values have been read. If ML F.A.M.E. communicates with a sample pipetting device, the barcode files (i.e. the barcodes of the samples being pipetted into the wells of the MTP) are combined with the results of the patients and result protocols are either printed out or sent to external communication systems (HOST, LAN).

1-16