Hamilton MICROLAB 500, MICROLAB 540B, MICROLAB 510B, MICROLAB 511C, MICROLAB 531C User Manual

Artisan Technology Group is your source for quality

new and certied-used/pre-owned equipment

• FAST SHIPPING AND

DELIVERY

• TENS OF THOUSANDS OF
IN-STOCK ITEMS

• EQUIPMENT DEMOS

• HUNDREDS OF
MANUFACTURERS

SUPPORTED

• LEASING/MONTHLY
RENTALS

• ITAR CERTIFIED
SECURE ASSET SOLUTIONS

SERVICE CENTER REPAIRS

Experienced engineers and technicians on staff
at our full-service, in-house repair center

Instra

Remotely inspect equipment before purchasing with
our interactive website at www.instraview.com

Contact us: (888) 88-SOURCE | [e-mail] | www.artisantg.com

SM

REMOTE INSPECTION

View

WE BUY USED EQUIPMENT

Sell your excess, underutilized, and idle used equipment
We also offer credit for buy-backs and trade-ins

www.artisantg.com/WeBuyEquipment

LOOKING FOR MORE INFORMATION?

Visit us on the web at www.artisantg.com for more
information on price quotations, drivers, technical

specications, manuals, and documentation

R

USERS

’

MANUAL

®

MICROLAB 500B/C

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

The MICROLAB ® 500 Series:

MICROLAB 510B, 530B, and 540B

and MICROLAB 511C, 531C, and 541C

User’s Manual

R

THE M

EASURE OF EXCELLENCE

Part Number 69176 (Rev. D)

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

SM

.

B Hamilton Company Instrument Warranty

Hamilton Company warrants this equipment (except valves*) to be free of defects in material
and workmanship for 12 months from the date of receipt. This warranty is extended to the buyer
of record on the original purchase order to Hamilton Company. Hamilton Company or an
authorized Hamilton representative agrees to repair or replace, at its option and free of charge
to the buyer at a normal place of business or at a Hamilton repair facility, any part or parts that
under proper and normal use prove to be defective during the warranty period.** Abuse,
unauthorized replacement of parts, modifications, or adjustments made by other than Company
or its assigned representatives voids this warranty.

This warranty gives you specific rights. No other warranties, expressed or implied, including
implications of warranties of merchantability and fitness for a particular product, are made.
Hamilton Company's liability on the sale of all products shall be limited to repair, replacement,
or refund of price of any defective product.**

Hamilton Company endeavors to provide prompt and satisfactory service.
*All Hamilton Company valves are warranted to be free of defects in material and

workmanship at the time of delivery.
**Hamilton Company reserves the right to refuse to accept the return of any instrument or valve

that has been used with radioactive or microbiological substances, or any other material that
may be deemed hazardous to employees of Hamilton Company.

© July 1999 by Hamilton Company
GASTIGHT is a registered trademark of Hamilton Company.
MICROLAB is a registered trademark licensed to Hamilton Company.
SANI-CLOTH is a registered trademark of Professional Disposables, Inc.

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Instruction to the User

This equipment has been tested and found to comply with the limits for a class B
digital device, pursuant to part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference in an installation. This
equipment generates, uses, and can radiate radio frequency energy, and if not
installed and used in accordance with the instructions, may cause harmful
interference to radio communications. However, there is no guarantee that
interference will not occur in a particular installation. If this equipment does cause
harmful interference to radio or television reception, which can be determined by
turning the equipment off and on, the user is encouraged to try to correct the
interference by one or more of the following measures:

• Reorient or relocate the receiving antenna.

• Increase the separation between the equipment and receiver.

• Connect the equipment into an outlet on a circuit different from that to which
the receiver is connected.

• Consult the dealer or an experienced radio/TV technician for help.

This equipment has been verified to comply with the limits for a class B
computing device, pursuant to FCC Rules. In order to maintain compliance
with the FCC regulations, shielded cables must be used with this equipment .
Operation with non-approved equipment or unshielded cables is likely to
result in interference to radio and TV reception. The user is cautioned that
changes and modifications made to the equipment without the approval of
the manufacturer could void the user’s authority to operate this equipment.

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Contents

Figures and Tables..................................................................xi

Preface ....................................................................PR–1

About the MICROLAB 500 Series of Instruments.....................PR–2

Upgrading Your MICROLAB 500 System..............................PR–3

About This Manual.................................................................PR–4

Conventions Used in This Manual ...........................................PR–5

A Word About Single Syringe Instruments ...............................PR–5

Chapter 1 Getting Started..................................................1–1

MICROLAB 500 Parts Lists.......................................................1 –2

A Brief Introduction to the MICROLAB 500B..............................1 – 8

Drive Unit..............................................................................1–8

Power Cord Connector Receptacle ................................... 1–13

Hand Probe or Footswitch Connector Receptacle..............1–13

Fuse Box.........................................................................1–13

Communications Settings.................................................1–13

Power On/Off Switch and Power Indicator Light.................1–14

Step/Prime Switch...........................................................1–14

Valve Assembly...............................................................1–15

Syringe Drive Arms........................................................... 1–15

Controller Unit........................................................................1–16

Pipettors/Probes................................................................... 1–18

Concorde Push-button Hand Pipettor/Probe.....................1–18

Dual Push-button Hand Pipettor/Probe ............................. 1–18

v

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Disposable Tip Push-button Hand Pipettor/Probes ............ 1–19

Luer Lock Tip Push-button Pipette Hand Pipettor/Probe....1–19

Viscous Sample Push-button Hand Pipette

Pipettor/Probe.................................................................1–20

Footswitch..........................................................................1–20

Probe Button Functions ...................................................... 1–20

Chapter 2 Installing the MICROLAB 500 System ....................2–1

Overview of Installation Procedures...........................................2–3

Selecting a Location.................................................................2 – 3

Installing the Accessory Holder.................................................2 – 3

Selecting Communications Options.......................................... 2– 4

Installing Electrical Connections................................................2–6

Installing Valve Assemblies.......................................................2–8

Installing the Valve Assembly on the ML 510B/511C...............2–8

Installing Valve Assemblies on the

ML530B/531C and 540B/541C ............................................. 2– 9

Selecting, Installing, and Removing Syringes...........................2–10

Preparing Syringes for Installation........................................2–12

Installing Syringes...............................................................2–12

Removing Syringes.............................................................2–14

Selecting and Installing Tubing...............................................2–16

Selecting Tubing................................................................ 2–16

Installing Tubing..................................................................2–17

Chapter 3 Programming and Using the

MICROLAB 500B System.....................................3–1

Using the Controller Unit...........................................................3–3

Using the Arrow Keys............................................................3 –3

vi MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Using the Numeric Keypad....................................................3–4

Using the Run/Stop Key........................................................3 – 4

Using the Function Keys.......................................................3– 4

Editing Conventions............................................................. 3– 5

Powering on the MICROLAB 500..............................................3 – 6

The Main Menu........................................................................3 –7

Priming the MICROLAB 500.....................................................3–8

Preparing to Prime................................................................3–8

Using the Prime Function from the Main Menu......................3–11

Creating a New Method..........................................................3–13

Creating an Aliquot Dispense Method..................................3–17

Creating a Serial Dispense Method.......................................3–18

Creating a Dilution Method...................................................3–20

Creating a Pipette Method...................................................3–23

Creating a Titrate Method.....................................................3–25

Creating a Custom Method..................................................3–28

Running an Existing Method...................................................3 –30

Editing an Existing Method .....................................................3–32

Performing Manual Dilutions...................................................3–33

Performing Manual Dispenses................................................3–35

The Utilities Menu..................................................................3–37

Renaming an Existing Method.............................................3 –38

Deleting an Existing Method ................................................3–40

Copying a Method to a Custom Method................................3–41

Downloading a Method to the Drive unit................................3–42

Diagnostic and Configuration Menu......................................3–44

Changing the Default Language.......................................3–44

Contents vii

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Setting the Baud Rate to the Drive Unit..............................3–45

Turning Caps Lock On and Off..........................................3–46

Performing the Display Test..............................................3–46

Performing the Keyboard Test .......................................... 3–46

Sample MICROLAB 510B Application Configurations...............3–47

Single Syringe Dispensing............................................ 3–47

Sample MICROLAB 530B Application Configurations...............3–48

Example 1: Dilutions......................................................3–48

Example 2: Dilutions......................................................3–49

Sample MICROLAB 540B Application Configurations...............3–50

Example 1: Dual Dispensing..........................................3–50

Example 2: Using the Dual Dispenser for

Single Dispensing......................................................... 3–50

Chapter 4 Caring for the MICROLAB 500..............................4–1

Deciding When to Clean the MICROLAB 500.............................4–2

Cleaning the Fluid Path of the MICROLAB 500..........................4 –2

Cleaning Syringes and Tubing..................................................4 – 3

Cleaning the Exterior of the MICROLAB 500..............................4–4

Chemical Compatibility..............................................................4–5

Storing the MICROLAB 500......................................................4 – 6

Replacing Batteries..................................................................4 – 6

Chapter 5 Troubleshooting the MICROLAB 500.................... 5 –1

Error Message Code Guide.......................................................5–2

Audible Messages.................................................................5–10

Troubleshooting Guide.......................................................... 5–11

Getting Technical Support ......................................................5–15

Returning Instruments for Repair.............................................5–16

viii MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Appendixes

Appendix A Technical Specifications for the MICROLAB 500........................A–1

Pin Outs for RS-232 and TTL Ports...........................................A –3

Appendix B Instrument Performance Test Reports.......................................B – 1

Appendix C Ordering Parts and Accessories for the MICROLAB 500.............C–1

Appendix D Chemical Compatibility of the MICROLAB 500............................ D –1

Appendix E Communication Protocols.........................................................E – 1

Hamilton Protocol 1/RNO+ Syntax Overview..........................E –1

Auto-addressing ................................................................E–2

Data Transfer Format ...........................................................E-4

Data String Components.....................................................E-5

DIN Protocol/BDZ+ Syntax..................................................... E–7

Hardwire-addressing..........................................................E – 8

Auto-addressing .................................................................E-8

Extablishing a Data Transfer Session....................................E-9

Terminating a Data Transfer Session................................... E-10

Data Transfer Session......................................................E–10

Data Block Format............................................................ E–10

Data String Components...................................................E-11

Broadcast Addressing ....................................................... E-16

Stored Methods................................................................E-16

Stored Method Definition..................................................E-16

Creating Stored Methods..................................................E-16

Stored Method Execution.................................................E-17

Appendix F Protocol Command Summary....................................................F –1

Channel Selection Commands.............................................F-2

Contents ix

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Initialization Commands .......................................................F-3

Syringe Positioning Commands ........................................... F-4

Valve Positioning Commands..............................................F-5

Timer and Digital I/O Commands...........................................F-6

Execution Commands ......................................................... F-7

Instrument Control Commands.............................................F-8

Syringe Parameter Change..................................................F-9

Valve Parameter Change...................................................F-10

Stored Method Parameter Change.....................................F-11

Instrument Information Requests.......................................F-11

Instrument Status Requests.............................................. F-13

Syringe Parameter Request...............................................F-19

Valve Parameter Request..................................................F-20

Timer and Digital I/O Requests...........................................F-21

Firmware Version Request.................................................F-22

ASCII Chart..........................................................................F-22

ML500B Default Environmental Parameters...........................F-26

ML500B Command Buffer .................................................... F-26

ML500B Methods ................................................................ F-27

ML500B Specific Protocol Information...................................F-27

Glossary ....................................................................GL–1

Index .....................................................................IN–1

x MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Figures and Tables

Preface

Figure PR–1 The MICROLAB 500 Series of Instruments.........PR–2

Chapter 1

Figure 1–1 MICROLAB 500B System Components...............1– 3

Figure 1–2 MICROLAB 500B Shipping Kit Components........1 –5

Figure 1–3 Small Parts Kit.....................................................1–6

Figure 1–4 Tubing Kit........................................................... 1 –7

Figure 1–5 Front View of theML510B Single

Syringe Dispenser.............................................. 1 – 9

Figure 1–6 Front View of the ML530B Dual Syringe Diluter... 1–10
Figure 1–7 Front View of the ML540B Dual

Syringe Dispenser............................................ 1–11

Figure 1–8 Rear View of the ML510B, 530B, and 540B........1–12

Figure 1–9 The Controller Unit............................................1–17

Figure 1–10 The Concorde, the Disposable Tip, and the Dual Hand

Pipettor/Probes ................................................1–19

Chapter 2

Table 1–1 MICROLAB 500 System Descriptions...................1–2

Table 1–2 MICROLAB 500B Shipping Kit............................. 1 – 4

Table 1–3 Small Parts Kit #35888 (For All Models).................1– 6

Table 1–4 Tubing Kit #35887 (For All Models)...................... 1– 7

Figure 2–1 Overview of Installation Procedures......................2 –2

Figure 2–2 Installing Electrical Connections...........................2 –7

Figure 2–3 Installing a Valve Assembly on the ML510B.......... 2– 8

Figures and Tables xi

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Figure 2–4 Installing a Valve Assembly on the

ML530B and 540B..............................................2 –9

Figure 2–5 The TLL-type Dispenser/Diluent Syringe............ 2–11

Figure 2–6 The TLLX-type Dispenser/Diluent Syringe..........2–11

Figure 2–7 The DX-type Sample Syringe.............................2–11

Figure 2–8 Installing a Syringe ............................................2–13

Figure 2–9 Removing a Syringe..........................................2–15

Figure 2–10 ML510B Valve and Tubing Connections............ 2–18

Figure 2–11 ML530B Valve with a D Configuration Syringe.....2–19

Figure 2–12 ML530B Valve with Two TLLX or TLL Syringes ... 2–19

Figure 2–13 ML540B Valve and Tubing Connectors .............. 2–20

Figure 2–14 Using the Tubing Reducer with the

Dual Hand Probe...............................................2–21

Table 2–1 Communications Switches and Ports....................2–4

Table 2–2 Reagent/Diluent Syringes

(TLL and TLLX-types)........................................2–10

Table 2–3 Sample Syringes (DX-type)................................2–11

Table 2–4 Tubing Selection Guide.....................................2–16

Chapter 3

Figure 3–1 Using the MICROLAB 500B.................................3–1

Figure 3–2 Overview of MICROLAB 500B Functions..............3–2

Figure 3–3 The Controller Unit..............................................3–3

Figure 3–4 Priming the MICROLAB 500..............................3–10

Figure 3–5 The Utilities Menu.............................................3–37

Figure 3–6 Single Syringe Dispensing ................................3–47

Figure 3–7 Dilutions........................................................... 3–48

Figure 3–8 Serial Dilutions..................................................3–49

Figure 3–9 Dual Dispensing................................................ 3–50

Figure 3–10 Using the Dual Dispenser for Single Dispensing.. 3–51

xii MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Chapter 5

Appendix A

Appendix B

Appendix C

Table 3–1 Function Key Actions .......................................... 3– 4

Table 5-1 Error Message Code Guide..................................5–2

Table 5–2 Audible System Messages ................................5–10

Table 5–3 Troubleshooting Guide .....................................5–11

Table A–1 Technical Specifications for the MICROLAB 500...A–1

Table A–2 Accuracy and Precision.......................................A–2

Figure B–1 Sample Performance Test Report........................B–2

Table C–1 Reagent/Diluent Syringe Replacement Parts ........C–1

Table C–2 Sample Syringe Replacement Parts .....................C –2

Table C–3 Valve Assemblies................................................C–2

Table C–4 Tubing ...............................................................C– 2

Table C–5 Pipettors/Probes ...............................................C –3

Table C–6 Parts and Accessories......................................... C –3

Appendix D

Appendix E

Appendix F

Table D–1 Chemical Compatibility.........................................D–1

Table E–1 Protocol 1/RNO+ Control Characters....................E– 2

Table E–2 DIN Protocol/BDZ+ Control Characters.................E– 8

Table F-1 Channel Selection Commands.............................F–2

Table F-2 Initialization Commands........................................ F-3

Table F-3 Syringe Positioning Commands............................ F-4

Figures and Tables xiii

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Table F-4 Valve Positioning Commands ...............................F-5

Table F-5 Timer and Digital I/O Commands............................F-6

Table F-6 Execution Commands.......................................... F-7

Table F-7 Instrument Control Commands.............................. F-8

Table F-8 Syringe Parameter Change................................... F-9

Table F-9 Valve Parameter Change.................................... F-10

Table F-10 Stored Method Parameter Change......................F-11

Table F-11 Instrument Information Requests ........................F-11

Table F-12 Instrument Status Request.................................F-13

Table F-13 Syringe Parameter Request................................ F-19

Table F-14 Valve Parameter Request...................................F-20

Table F-15 Timer and Digital I/O Requests ............................F-21

Table F-16 Firmware Version Request..................................F-22

Table F-17 ASCII Chart........................................................F-23

xiv MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Preface Welcome to the World of

Hamilton Precision
Instruments

Congratulations on your purchase of a Hamilton MICROLAB 500
system. The Hamilton MICROLAB 500 is a versatile, semi­automatic, precision liquid processor. Various models of the
MICROLAB 500 function as either single- or dual-syringe
diluter/dispensers.

The MICROLAB 500 functions on the principal of liquid/liquid
displacement. At the heart of each MICROLAB 500 system is a
highly efficient, precision stepper motor drive that is combined
with world-famous Hamilton GASTIGHT syringes. The result is
a precise and accurate instrument that is very easy to set up
and use.

With proper care and maintenance, your new MICROLAB 500
system will serve you faithfully. To learn about the proper care
and maintenance of your investment, please take the time to read
this manual. Also, please read the warranty information that
appears on the copyright page in this manual and on the
separate warranty sheet that is included in your MICROLAB 500
shipping kit.

The Hamilton Company thanks you for purchasing this
Hamilton product. Welcome to the world of Hamilton precision
instruments!

PR–1

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

About the MICROLAB 500 Series of Instruments

All of the MICROLAB 500 systems feature four common pipette modes: fill,
dispense, auto-refill, and prime. The systems are capable of performing accurate
and precise transfer pipetting, and of performing automated dilutions up to 1:25,000.
The instruments can also dispense up to 50 mL per cycle. Figure PR–1 provides an
overview of the MICROLAB 500 Series of Instruments.

Figure PR–1 The MICROLAB 500 Series of Instruments

The Microlab 500 Series of Instruments

Single Syringe Instruments

Controller without memory:

• Microlab 501A dispenser

Controller with memory:

• Microlab 510B diluter/dispenser

Computer-controlled:

• Microlab 511C diluter/dispenser

Dual Syringe Instruments

Controller without memory:

• Microlab 503A diluter

• Microlab 504A dispenser

Controller with memory:

• Microlab 530B diluter/dispenser

• Microlab 540B dispenser

Computer-controlled:

• Microlab 531C diluter/dispenser

• Microlab 541C dispenser

PR–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

The MICROLAB 500 Series of instruments consists of three different lines of
diluter/dispensers.

• Controller without memory: Use the controller unit to manually enter methods.

This controller unit does not have memory, so methods cannot be stored.

• Controller with memory: This controller unit has memory, allowing you to

program and store your own custom methods. Or, use the controller unit to run
manual methods. Any instrument that uses this controller can also be computer
controlled.

• Computer controlled: No controller unit is included; use a computer to operate

the instrument.

The MICROLAB 510B/511C, 530B/531C, and 540B/541C are described in this
manual.

• The MICROLAB 510B/511C is a single syringe dispenser designed for single

precision dispensing applications.

• The MICROLAB 530B/531C is a dual syringe diluter designed for repetitive

dilution applications.

• The MICROLAB 540B/541C is a dual syringe dispenser designed for precision

dispensing applications that require more than one liquid to be dispensed at a
time.

Upgrading Your MICROLAB 500 System

Both the MICROLAB 510B/511C and 530B/531C systems can be upgraded to
MICROLAB 540B/541C systems. For upgrade information or for information about
purchasing any of the MICROLAB 500 models, contact your authorized Hamilton
sales representative or contact Hamilton Company.

In the United States:

Hamilton Company, P.O. Box 10030, Reno, Nevada 89520–0012
Telephone Numbers (in the USA and Canada):
Technical/Customer Service 1–800–648–5950 8 a.m. to 5 p.m. PST
Instrument Service 1–800–527–5269

Preface PR–3

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Outside the USA and Canada:

[phone]

Fax Number: [phone]

In Switzerland:

Hamilton Bonaduz AG, Ch–7402, P.O. Box 26,
Bonaduz, Switzerland
Telephone Number: [phone]
Fax Number: [phone]

About This Manual

This manual provides technical information about the MICROLAB 510B/511C,
530B/531C, and 540B/541C, and is divided into chapters that cover the following
topics:

• Chapter 1, Getting Started, provides an overview of the MICROLAB 500
system, including a complete parts list and a brief description of the system
components.

• Chapter 2, Installing the MICROLAB 500, describes how to set up the system.

• Chapter 3, Using the MICROLAB 500, provides step-by-step instructions for
using the system. It also provides sample applications.

• Chapter 4, Caring for the MICROLAB 500, describes everyday maintenance
techniques.

• Chapter 5, Troubleshooting, contains tables that list system messages and their
meanings, and common problems and their solutions.

• The Appendixes provide detailed information, such as technical specifications,
ordering information, etc.

• The Glossary defines terms used in this manual.

• The Index provides a quick-reference to the topics described in this manual.

PR–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Conventions Used in This Manual

Throughout this manual symbols are used to call your attention to various kinds of
information.

A Warning! Information that is essential for avoiding personal

injury is flagged with the International Warning
Symbol and appears like this in the text.

▲ Important Information that is essential for avoiding damage to

equipment appears like this in the text.

✱ Note: Interesting information or information that can help

improve system performance appears like this in the text. ✱

System messages and prompts that appear on the controller unit’s display screen
are shown in courier font. Items that are selected and user input appear in

boldface courier.

A

▲

A Word About Single Syringe Instruments

Throughout this manual you will see references to multiple syringes, volumes,
speeds, and to the right-side controls. All screen examples show the use of dual
syringe instruments. If you are using a MICROLAB 510B/511C single syringe
dispenser, please disregard these references. The right-side functions are not
available on single syringe instruments.

Any operational differences between the single and the dual syringe instruments
are called out in the text.

Preface PR–5

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Chapter 1 Getting Started

This chapter provides a brief overview of the MICROLAB 500
system. Information in this chapter includes:

• MICROLAB 500 parts lists

• MICROLAB 500 components
– the drive unit
– the controller unit
– hand pipettors/probes

All MICROLAB 500 instruments come with everything you need
to start using the system, with the exception of syringes. You
must separately purchase syringes for use with the MICROLAB
500 systems. For complete lists of syringes, accessories, and
replacement parts for the MICROLAB 500, see Appendix C,
Ordering Parts and Accessories for the MICROLAB 500.

✱ Note: Contact your local delivery company if you

notice any visual damage to the MICROLAB 500
shipping package or to its contents. Also, you
may want to save the shipping container in case
you ever need to return the instrument for

service. ✱

1–1

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

MICROLAB 500 Parts Lists

This section includes complete parts lists for the MICROLAB 500 systems. After you
unpack your MICROLAB 500, check to see that you have received all parts before
attempting to set up the system. The parts lists are presented in four separate tables
with four corresponding figures.

Table 1–1 lists the programmable models in the MICROLAB 500 series, their
components, and each component’s part number. Figure 1–1 shows these components.

Table 1–1 MICROLAB 500 System Descriptions

Model* Part # Drive

MICROLAB
510B 115V

MICROLAB
510B 220V

MICROLAB
530B 115V

MICROLAB
530B 220V

MICROLAB
540B 115V

MICROLAB
540B 220V

ML510115 35890 35893 69176 (English)

ML510220 35890 35893 same as above 355010 35792

ML530115 35891 35893 same as above 6541000 35793

ML530220 35891 35893 same as above 355010 35793

ML540115 35892 35893 same as above 6541000 35794

ML540220 35892 35893 same as above 355010 35794

Unit

Part #

Controller

Unit &

Cord

Part #

Manual Part # Power

69182 (French)

69180 (German)

69186 (Portuguese)

69188 (Spanish)

Shipping

Cord

6541000 35792

Kit

Part #

* All MICROLAB 500C models come with the drive unit, the manual, a power cord,
and a valve. The MICROLAB 500 C does not come with a controller or a shipping
kit.

1–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Figure 1–1 MICROLAB 500 System Components (items shown are

R

R

not to scale)

R

R

1

2

3

4

5

6

7

8

9

.

STEP

PRIME

POWER

Dual Drive Unit

Part # 35891 or 35892

(Microlab 530B/531C or 540B/541C)

0

Controller Unit and Cord

Part # 35893

(not with 500C models)

Stop

Run

R

R

PRIME

POWER

Single Drive Unit

Part # 35890

(Microlab 510B/511C)

Power Cord

Part # 6541000 or 355010

Shipping Kit
Part # 35792 (Microlab 510B)
Part # 35793 (Microlab 530B)
Part # 35794 (Microlab 540B)

STEP

R

Manual

Part # 69176

Chapter 1 Getting Started 1–3

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Table 1–2 lists the components that make up the MICROLAB 500B Shipping Kit
(shown as a box in Figure 1–1). Figure 1–2 shows these components.

Table 1–2 MICROLAB 500B Shipping Kit

Shipping Kit*

Part #

MICROLAB

510B

35792

MICROLAB

530B

35793

MICROLAB

540B

35794

Valve Part # Hand Probe Part

#

HV Valve

35825

Concorde

Probe
35529

Diluter

Valve

35844

Dispenser

Valve

35842

Concorde

Probe
35529

Dual Hand

Probe
35767

Small Parts Kit

Part #

35888 35887

35888 35887

35888 35887

Tubing Kit

Part #

2 items

*The MICROLAB 500C is standard with only the valve. All other parts must be
ordered separately.

1–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Figure 1–2 MICROLAB 500B Shipping Kit Components (items

R

shown are not to scale)

Small Parts Kit

Part # 35888

Concorde Probe

(Microlab 510B/511C or 530B/531C)

Part # 35529

Tubing Kit

Part # 35887

Dual Hand Probe

Part # 35767

(Microlab 540B/541C)

HV Valve

Part # 35825

(Microlab 510B/511C)

Dispenser Valve

Part # 35842

Diluter Valve
Part # 35844

Chapter 1 Getting Started 1–5

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Table 1–3 lists the components that make up the MICROLAB 500B Small Parts Kit
(shown as a box in Figure 1–2). Figure 1–3 shows these components.

Table 1–3 Small Parts Kit #35888 (For All 500B Models)

Tubing Clips

Part #

230010

2 items

1 AMP Fuses

Part #

1524-01

2 items

Accessory

Holder

Part #

35783

1 item

Screws

Part #

16500

2 items

Figure 1–3 Small Parts Kit (items shown are not to scale)

(2) Tubing Clips

Part # 230010

Tubing Reducers

Part # 35770

Accessory

Holder

Part # 35783

Tubing

Reducers

Part #

35770

2 items

(2) Screws

Part # 16500

(2) Fuses

Part # 1524-01

1–6 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Table 1–4 lists the components that make up the MICROLAB 500 Tubing Kit (shown
as a box in Figure 1–2). Figure 1–4 shows these components.

Table 1–4 Tubing Kit #35887 (For All 500B Models)

12 ga. x 650 mm

Fill Tubing (not

tapered)

Part #

18 ga. x 650 mm

Fill Tubing (not

tapered)

Part #

12 ga. x 900 mm

Dispense Tubing

(tapered)

Part #

18 ga. x 900 mm

Dispense Tubing

(tapered)

Part #

240000* 240010* 240360* 240130*

* M6 threaded hubs are used on all tubing.

Figure 1–4 Tubing Kit (items shown are not to scale)

12 ga. Fill Tubing

Part # 240000

12 ga. Dispense Tubing

Part # 240360

18 ga. Fill Tubing

Part # 240010

18 ga. Dispense Tubing

Part # 240130

For complete lists of syringes, accessories, and replacement parts for the
MICROLAB 500, see Appendix C, Ordering Parts and Accessories for the
MICROLAB 500.

Chapter 1 Getting Started 1–7

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

A Brief Introduction to the MICROLAB 500B

The MICROLAB 510B, 530B, and 540B systems each consist of three basic units.
These units include:

• a drive unit

• a controller unit

• a hand probe
This section briefly describes these units and the individual components that

comprise each unit. See Chapter 2, Installing the MICROLAB 500, for complete
installation instructions; see Chapter 3, Using the MICROLAB 500, for complete
usage instructions.

Drive Unit

The drive unit is the heart of each MICROLAB 500 system. The drive unit contains
a precision drive motor, the syringe drive arms, the valve assembly, the power
switches, and the connector receptacles. These features allow you to control other
sub-assemblies, and together they create a very versatile and functional
instrument.

Figure 1– 5 shows the front view of the MICROLAB 510B single syringe
diluter/dispenser. In this figure, the controller unit rests on top of the drive unit and
a syringe is attached to the syringe drive arm.

A Warning! This warning label appears on the front panel of the

ML500. It indicates that a pinch hazard exists when
the syringe drive is moving.

1–8 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

A