Hamilton Microlab 500 User manual

Download

USERS

’

MANUAL

MICROLAB 500B/C

The MICROLAB ® 500 Series:

MICROLAB 510B, 530B, and 540B

and MICROLAB 511C, 531C, and 541C

User’s Manual

THE M

EASURE OF EXCELLENCE

Part Number 69176 (Rev. D)

B Hamilton Company Instrument Warranty

Hamilton Company warrants this equipment (except valves*) to be free of defects in material and workmanship for 12 months from the date of receipt. This warranty is extended to the buyer of record on the original purchase order to Hamilton Company. Hamilton Company or an authorized Hamilton representative agrees to repair or replace, at its option and free of charge to the buyer at a normal place of business or at a Hamilton repair facility, any part or parts that under proper and normal use prove to be defective during the warranty period.** Abuse, unauthorized replacement of parts, modifications, or adjustments made by other than Company or its assigned representatives voids this warranty.

This warranty gives you specific rights. No other warranties, expressed or implied, including implications of warranties of merchantability and fitness for a particular product, are made. Hamilton Company's liability on the sale of all products shall be limited to repair, replacement, or refund of price of any defective product.**

Hamilton Company endeavors to provide prompt and satisfactory service. *All Hamilton Company valves are warranted to be free of defects in material and

workmanship at the time of delivery. **Hamilton Company reserves the right to refuse to accept the return of any instrument or valve

that has been used with radioactive or microbiological substances, or any other material that may be deemed hazardous to employees of Hamilton Company.

© July 1999 by Hamilton Company GASTIGHT is a registered trademark of Hamilton Company. MICROLAB is a registered trademark licensed to Hamilton Company. SANI-CLOTH is a registered trademark of Professional Disposables, Inc.

Instruction to the User

This equipment has been tested and found to comply with the limits for a class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in an installation. This equipment generates, uses, and can radiate radio frequency energy, and if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

• Reorient or relocate the receiving antenna.

• Increase the separation between the equipment and receiver.

• Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.

• Consult the dealer or an experienced radio/TV technician for help.

This equipment has been verified to comply with the limits for a class B computing device, pursuant to FCC Rules. In order to maintain compliance with the FCC regulations, shielded cables must be used with this equipment . Operation with non-approved equipment or unshielded cables is likely to result in interference to radio and TV reception. The user is cautioned that changes and modifications made to the equipment without the approval of the manufacturer could void the user’s authority to operate this equipment.

Contents

Figures and Tables..................................................................xi

Preface ....................................................................PR–1

About the MICROLAB 500 Series of Instruments.....................PR–2

Upgrading Your MICROLAB 500 System..............................PR–3

About This Manual.................................................................PR–4

Conventions Used in This Manual ...........................................PR–5

A Word About Single Syringe Instruments ...............................PR–5

Chapter 1 Getting Started..................................................1–1

MICROLAB 500 Parts Lists.......................................................1–2

A Brief Introduction to the MICROLAB 500B..............................1 –8

Drive Unit..............................................................................1–8

Power Cord Connector Receptacle ................................... 1–13

Hand Probe or Footswitch Connector Receptacle..............1–13

Fuse Box.........................................................................1–13

Communications Settings.................................................1–13

Power On/Off Switch and Power Indicator Light.................1–14

Step/Prime Switch...........................................................1–14

Valve Assembly...............................................................1–15

Syringe Drive Arms........................................................... 1–15

Controller Unit........................................................................1–16

Pipettors/Probes...................................................................1–18

Concorde Push-button Hand Pipettor/Probe.....................1–18

Dual Push-button Hand Pipettor/Probe ............................. 1–18

Disposable Tip Push-button Hand Pipettor/Probes ............ 1–19

Luer Lock Tip Push-button Pipette Hand Pipettor/Probe....1–19

Viscous Sample Push-button Hand Pipette

Pipettor/Probe.................................................................1–20

Footswitch..........................................................................1–20

Probe Button Functions ...................................................... 1–20

Chapter 2 Installing the MICROLAB 500 System ....................2–1

Overview of Installation Procedures...........................................2–3

Selecting a Location.................................................................2–3

Installing the Accessory Holder.................................................2– 3

Selecting Communications Options..........................................2 – 4

Installing Electrical Connections................................................2 –6

Installing Valve Assemblies.......................................................2–8

Installing the Valve Assembly on the ML 510B/511C...............2 – 8

Installing Valve Assemblies on the

ML530B/531C and 540B/541C ............................................. 2– 9

Selecting, Installing, and Removing Syringes...........................2–10

Preparing Syringes for Installation........................................2–12

Installing Syringes...............................................................2–12

Removing Syringes.............................................................2–14

Selecting and Installing Tubing...............................................2–16

Selecting Tubing................................................................2–16

Installing Tubing..................................................................2–17

Chapter 3 Programming and Using the

MICROLAB 500B System.....................................3–1

Using the Controller Unit...........................................................3–3

Using the Arrow Keys............................................................3–3

vi MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Using the Numeric Keypad....................................................3–4

Using the Run/Stop Key........................................................3 – 4

Using the Function Keys.......................................................3 – 4

Editing Conventions............................................................. 3 –5

Powering on the MICROLAB 500..............................................3 – 6

The Main Menu........................................................................3–7

Priming the MICROLAB 500.....................................................3–8

Preparing to Prime................................................................3–8

Using the Prime Function from the Main Menu......................3–11

Creating a New Method..........................................................3–13

Creating an Aliquot Dispense Method..................................3–17

Creating a Serial Dispense Method.......................................3–18

Creating a Dilution Method...................................................3–20

Creating a Pipette Method...................................................3–23

Creating a Titrate Method.....................................................3–25

Creating a Custom Method..................................................3–28

Running an Existing Method...................................................3 –30

Editing an Existing Method .....................................................3–32

Performing Manual Dilutions...................................................3–33

Performing Manual Dispenses................................................3–35

The Utilities Menu..................................................................3–37

Renaming an Existing Method.............................................3 –38

Deleting an Existing Method ................................................3–40

Copying a Method to a Custom Method................................3–41

Downloading a Method to the Drive unit................................3–42

Diagnostic and Configuration Menu......................................3–44

Changing the Default Language.......................................3–44

Contents vii

Setting the Baud Rate to the Drive Unit..............................3–45

Turning Caps Lock On and Off..........................................3–46

Performing the Display Test..............................................3–46

Performing the Keyboard Test .......................................... 3–46

Sample MICROLAB 510B Application Configurations...............3–47

Single Syringe Dispensing............................................3–47

Sample MICROLAB 530B Application Configurations...............3–48

Example 1: Dilutions......................................................3–48

Example 2: Dilutions......................................................3–49

Sample MICROLAB 540B Application Configurations...............3–50

Example 1: Dual Dispensing..........................................3–50

Example 2: Using the Dual Dispenser for

Single Dispensing......................................................... 3–50

Chapter 4 Caring for the MICROLAB 500..............................4–1

Deciding When to Clean the MICROLAB 500.............................4–2

Cleaning the Fluid Path of the MICROLAB 500..........................4 –2

Cleaning Syringes and Tubing..................................................4 – 3

Cleaning the Exterior of the MICROLAB 500..............................4– 4

Chemical Compatibility..............................................................4– 5

Storing the MICROLAB 500......................................................4 – 6

Replacing Batteries..................................................................4–6

Chapter 5 Troubleshooting the MICROLAB 500.................... 5 –1

Error Message Code Guide.......................................................5–2

Audible Messages.................................................................5–10

Troubleshooting Guide.......................................................... 5–11

Getting Technical Support ......................................................5–15

Returning Instruments for Repair.............................................5–16

viii MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Appendixes

Appendix A Technical Specifications for the MICROLAB 500........................ A –1

Pin Outs for RS-232 and TTL Ports........................................... A–3

Appendix B Instrument Performance Test Reports.......................................B –1

Appendix C Ordering Parts and Accessories for the MICROLAB 500.............C–1

Appendix D Chemical Compatibility of the MICROLAB 500............................ D –1

Appendix E Communication Protocols.........................................................E – 1

Hamilton Protocol 1/RNO+ Syntax Overview.......................... E –1

Auto-addressing ................................................................E–2

Data Transfer Format ...........................................................E-4

Data String Components..................................................... E-5

DIN Protocol/BDZ+ Syntax..................................................... E–7

Hardwire-addressing..........................................................E–8

Auto-addressing .................................................................E-8

Extablishing a Data Transfer Session....................................E-9

Terminating a Data Transfer Session................................... E-10

Data Transfer Session......................................................E–10

Data Block Format............................................................E–10

Data String Components................................................... E-11

Broadcast Addressing ....................................................... E-16

Stored Methods................................................................E-16

Stored Method Definition..................................................E-16

Creating Stored Methods.................................................. E-16

Stored Method Execution.................................................E-17

Appendix F Protocol Command Summary....................................................F–1

Channel Selection Commands.............................................F-2

Contents ix

Initialization Commands .......................................................F-3

Syringe Positioning Commands ........................................... F-4

Valve Positioning Commands..............................................F-5

Timer and Digital I/O Commands...........................................F-6

Execution Commands ......................................................... F-7

Instrument Control Commands.............................................F-8

Syringe Parameter Change..................................................F-9

Valve Parameter Change...................................................F-10

Stored Method Parameter Change.....................................F-11

Instrument Information Requests.......................................F-11

Instrument Status Requests..............................................F-13

Syringe Parameter Request...............................................F-19

Valve Parameter Request..................................................F-20

Timer and Digital I/O Requests...........................................F-21

Firmware Version Request.................................................F-22

ASCII Chart..........................................................................F-22

ML500B Default Environmental Parameters...........................F-26

ML500B Command Buffer .................................................... F-26

ML500B Methods ................................................................ F-27

ML500B Specific Protocol Information...................................F-27

Glossary ....................................................................GL–1

Index .....................................................................IN–1

x MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figures and Tables

Preface

Figure PR–1 The MICROLAB 500 Series of Instruments.........PR–2

Chapter 1

Figure 1–1 MICROLAB 500B System Components...............1–3

Figure 1–2 MICROLAB 500B Shipping Kit Components........1– 5

Figure 1–3 Small Parts Kit.....................................................1 –6

Figure 1–4 Tubing Kit........................................................... 1 –7

Figure 1–5 Front View of theML510B Single

Syringe Dispenser..............................................1 – 9

Figure 1–6 Front View of the ML530B Dual Syringe Diluter... 1–10 Figure 1–7 Front View of the ML540B Dual

Syringe Dispenser............................................1–11

Figure 1–8 Rear View of the ML510B, 530B, and 540B........1–12

Figure 1–9 The Controller Unit............................................1–17

Figure 1–10 The Concorde, the Disposable Tip, and the Dual Hand

Pipettor/Probes ................................................1–19

Chapter 2

Table 1–1 MICROLAB 500 System Descriptions...................1 – 2

Table 1–2 MICROLAB 500B Shipping Kit............................. 1 – 4

Table 1–3 Small Parts Kit #35888 (For All Models).................1–6

Table 1–4 Tubing Kit #35887 (For All Models)...................... 1 – 7

Figure 2–1 Overview of Installation Procedures......................2–2

Figure 2–2 Installing Electrical Connections...........................2–7

Figure 2–3 Installing a Valve Assembly on the ML510B..........2–8

Figures and Tables xi

Figure 2–4 Installing a Valve Assembly on the

ML530B and 540B..............................................2 –9

Figure 2–5 The TLL-type Dispenser/Diluent Syringe............ 2–11

Figure 2–6 The TLLX-type Dispenser/Diluent Syringe.......... 2–11

Figure 2–7 The DX-type Sample Syringe.............................2–11

Figure 2–8 Installing a Syringe ............................................2–13

Figure 2–9 Removing a Syringe..........................................2–15

Figure 2–10 ML510B Valve and Tubing Connections ............ 2–18

Figure 2–11 ML530B Valve with a D Configuration Syringe.....2–19

Figure 2–12 ML530B Valve with Two TLLX or TLL Syringes...2–19

Figure 2–13 ML540B Valve and Tubing Connectors .............. 2–20

Figure 2–14 Using the Tubing Reducer with the

Dual Hand Probe...............................................2–21

Table 2–1 Communications Switches and Ports....................2 –4

Table 2–2 Reagent/Diluent Syringes

(TLL and TLLX-types)........................................2–10

Table 2–3 Sample Syringes (DX-type)................................2–11

Table 2–4 Tubing Selection Guide.....................................2–16

Chapter 3

Figure 3–1 Using the MICROLAB 500B.................................3– 1

Figure 3–2 Overview of MICROLAB 500B Functions..............3– 2

Figure 3–3 The Controller Unit..............................................3– 3

Figure 3–4 Priming the MICROLAB 500..............................3–10

Figure 3–5 The Utilities Menu.............................................3–37

Figure 3–6 Single Syringe Dispensing ................................ 3–47

Figure 3–7 Dilutions ........................................................... 3–48

Figure 3–8 Serial Dilutions..................................................3–49

Figure 3–9 Dual Dispensing................................................ 3–50

Figure 3–10 Using the Dual Dispenser for Single Dispensing.. 3–51

xii MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Chapter 5

Appendix A

Appendix B

Appendix C

Table 3–1 Function Key Actions .......................................... 3– 4

Table 5-1 Error Message Code Guide..................................5–2

Table 5–2 Audible System Messages ................................5–10

Table 5–3 Troubleshooting Guide .....................................5–11

Table A–1 Technical Specifications for the MICROLAB 500...A–1

Table A–2 Accuracy and Precision.......................................A– 2

Figure B–1 Sample Performance Test Report........................B–2

Table C–1 Reagent/Diluent Syringe Replacement Parts ........C–1

Table C–2 Sample Syringe Replacement Parts .....................C–2

Table C–3 Valve Assemblies................................................C–2

Table C–4 Tubing ............................................................... C –2

Table C–5 Pipettors/Probes ...............................................C –3

Table C–6 Parts and Accessories.........................................C–3

Appendix D

Appendix E

Appendix F

Table D–1 Chemical Compatibility.........................................D–1

Table E–1 Protocol 1/RNO+ Control Characters....................E – 2

Table E–2 DIN Protocol/BDZ+ Control Characters.................E–8

Table F-1 Channel Selection Commands.............................F–2

Table F-2 Initialization Commands........................................F-3

Table F-3 Syringe Positioning Commands............................ F-4

Figures and Tables xiii

Table F-4 Valve Positioning Commands ............................... F-5

Table F-5 Timer and Digital I/O Commands............................F-6

Table F-6 Execution Commands.......................................... F-7

Table F-7 Instrument Control Commands.............................. F-8

Table F-8 Syringe Parameter Change................................... F-9

Table F-9 Valve Parameter Change.................................... F-10

Table F-10 Stored Method Parameter Change......................F-11

Table F-11 Instrument Information Requests ........................F-11

Table F-12 Instrument Status Request.................................F-13

Table F-13 Syringe Parameter Request................................ F-19

Table F-14 Valve Parameter Request...................................F-20

Table F-15 Timer and Digital I/O Requests ............................F-21

Table F-16 Firmware Version Request..................................F-22

Table F-17 ASCII Chart........................................................F-23

xiv MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Preface Welcome to the World of

Hamilton Precision Instruments

Congratulations on your purchase of a Hamilton MICROLAB 500 system. The Hamilton MICROLAB 500 is a versatile, semiautomatic, precision liquid processor. Various models of the MICROLAB 500 function as either single- or dual-syringe diluter/dispensers.

The MICROLAB 500 functions on the principal of liquid/liquid displacement. At the heart of each MICROLAB 500 system is a highly efficient, precision stepper motor drive that is combined with world-famous Hamilton GASTIGHT syringes. The result is a precise and accurate instrument that is very easy to set up and use.

With proper care and maintenance, your new MICROLAB 500 system will serve you faithfully. To learn about the proper care and maintenance of your investment, please take the time to read this manual. Also, please read the warranty information that appears on the copyright page in this manual and on the separate warranty sheet that is included in your MICROLAB 500 shipping kit.

The Hamilton Company thanks you for purchasing this Hamilton product. Welcome to the world of Hamilton precision instruments!

PR–1

About the MICROLAB 500 Series of Instruments

All of the MICROLAB 500 systems feature four common pipette modes: fill, dispense, auto-refill, and prime. The systems are capable of performing accurate and precise transfer pipetting, and of performing automated dilutions up to 1:25,000. The instruments can also dispense up to 50 mL per cycle. Figure PR–1 provides an overview of the MICROLAB 500 Series of Instruments.

Figure PR–1 The MICROLAB 500 Series of Instruments

The Microlab 500 Series of Instruments

Single Syringe Instruments

Controller without memory:

• Microlab 501A dispenser

Controller with memory:

• Microlab 510B diluter/dispenser

Computer-controlled:

• Microlab 511C diluter/dispenser

Dual Syringe Instruments

Controller without memory:

• Microlab 503A diluter

• Microlab 504A dispenser

Controller with memory:

• Microlab 530B diluter/dispenser

• Microlab 540B dispenser

Computer-controlled:

• Microlab 531C diluter/dispenser

• Microlab 541C dispenser

PR–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

The MICROLAB 500 Series of instruments consists of three different lines of diluter/dispensers.

• Controller without memory: Use the controller unit to manually enter methods.

This controller unit does not have memory, so methods cannot be stored.

• Controller with memory: This controller unit has memory, allowing you to

program and store your own custom methods. Or, use the controller unit to run manual methods. Any instrument that uses this controller can also be computer controlled.

• Computer controlled: No controller unit is included; use a computer to operate

the instrument.

The MICROLAB 510B/511C, 530B/531C, and 540B/541C are described in this manual.

• The MICROLAB 510B/511C is a single syringe dispenser designed for single

precision dispensing applications.

• The MICROLAB 530B/531C is a dual syringe diluter designed for repetitive

dilution applications.

• The MICROLAB 540B/541C is a dual syringe dispenser designed for precision

dispensing applications that require more than one liquid to be dispensed at a time.

Upgrading Your MICROLAB 500 System

Both the MICROLAB 510B/511C and 530B/531C systems can be upgraded to MICROLAB 540B/541C systems. For upgrade information or for information about purchasing any of the MICROLAB 500 models, contact your authorized Hamilton sales representative or contact Hamilton Company.

In the United States:

Hamilton Company, P.O. Box 10030, Reno, Nevada 89520–0012 Telephone Numbers (in the USA and Canada): Technical/Customer Service 1–800–648–5950 8 a.m. to 5 p.m. PST Instrument Service 1–800–527–5269

Preface PR–3

Outside the USA and Canada:

+1–775–858–3000

Fax Number: +1–775–856–7259

In Switzerland:

Hamilton Bonaduz AG, Ch–7402, P.O. Box 26, Bonaduz, Switzerland Telephone Number: +41–81–660–60–60 Fax Number: +41–81–660–60–70

About This Manual

This manual provides technical information about the MICROLAB 510B/511C, 530B/531C, and 540B/541C, and is divided into chapters that cover the following topics:

• Chapter 1, Getting Started, provides an overview of the MICROLAB 500 system, including a complete parts list and a brief description of the system components.

• Chapter 2, Installing the MICROLAB 500, describes how to set up the system.

• Chapter 3, Using the MICROLAB 500, provides step-by-step instructions for using the system. It also provides sample applications.

• Chapter 4, Caring for the MICROLAB 500, describes everyday maintenance techniques.

• Chapter 5, Troubleshooting, contains tables that list system messages and their meanings, and common problems and their solutions.

• The Appendixes provide detailed information, such as technical specifications, ordering information, etc.

• The Glossary defines terms used in this manual.

• The Index provides a quick-reference to the topics described in this manual.

PR–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Conventions Used in This Manual

Throughout this manual symbols are used to call your attention to various kinds of information.

A Warning! Information that is essential for avoiding personal

injury is flagged with the International Warning Symbol and appears like this in the text.

▲ Important Information that is essential for avoiding damage to

equipment appears like this in the text.

✱ Note: Interesting information or information that can help

improve system performance appears like this in the text. ✱

System messages and prompts that appear on the controller unit’s display screen are shown in courier font. Items that are selected and user input appear in

boldface courier.

▲

A Word About Single Syringe Instruments

Throughout this manual you will see references to multiple syringes, volumes, speeds, and to the right-side controls. All screen examples show the use of dual syringe instruments. If you are using a MICROLAB 510B/511C single syringe dispenser, please disregard these references. The right-side functions are not available on single syringe instruments.

Any operational differences between the single and the dual syringe instruments are called out in the text.

Preface PR–5

Chapter 1 Getting Started

This chapter provides a brief overview of the MICROLAB 500 system. Information in this chapter includes:

• MICROLAB 500 parts lists

• MICROLAB 500 components – the drive unit – the controller unit – hand pipettors/probes

All MICROLAB 500 instruments come with everything you need to start using the system, with the exception of syringes. You must separately purchase syringes for use with the MICROLAB 500 systems. For complete lists of syringes, accessories, and replacement parts for the MICROLAB 500, see Appendix C, Ordering Parts and Accessories for the MICROLAB 500.

✱ Note: Contact your local delivery company if you

notice any visual damage to the MICROLAB 500 shipping package or to its contents. Also, you may want to save the shipping container in case you ever need to return the instrument for

service. ✱

1–1

MICROLAB 500 Parts Lists

This section includes complete parts lists for the MICROLAB 500 systems. After you unpack your MICROLAB 500, check to see that you have received all parts before attempting to set up the system. The parts lists are presented in four separate tables with four corresponding figures.

Table 1–1 lists the programmable models in the MICROLAB 500 series, their components, and each component’s part number. Figure 1–1 shows these components.

Table 1–1 MICROLAB 500 System Descriptions

Model* Part # Drive

MICROLAB 510B 115V

MICROLAB 510B 220V

MICROLAB 530B 115V

MICROLAB 530B 220V

MICROLAB 540B 115V

MICROLAB 540B 220V

ML510115 35890 35893 69176 (English)

ML510220 35890 35893 same as above 355010 35792

ML530115 35891 35893 same as above 6541000 35793

ML530220 35891 35893 same as above 355010 35793

ML540115 35892 35893 same as above 6541000 35794

ML540220 35892 35893 same as above 355010 35794

Unit

Part #

Controller

Unit &

Cord

Part #

Manual Part # Power

69182 (French)

69180 (German)

69186 (Portuguese)

69188 (Spanish)

Shipping

Cord

6541000 35792

Kit

Part #

* All MICROLAB 500C models come with the drive unit, the manual, a power cord, and a valve. The MICROLAB 500 C does not come with a controller or a shipping kit.

1–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figure 1–1 MICROLAB 500 System Components (items shown are

not to scale)

STEP

PRIME

POWER

Dual Drive Unit

Part # 35891 or 35892

(Microlab 530B/531C or 540B/541C)

Controller Unit and Cord

Part # 35893

(not with 500C models)

Stop

Run

Power Cord

Part # 6541000 or 355010

Shipping Kit

Part # 35792 (Microlab 510B)

STEP

PRIME

POWER

Part # 35793 (Microlab 530B) Part # 35794 (Microlab 540B)

Single Drive Unit

Part # 35890

(Microlab 510B/511C)

Manual

Part # 69176

Chapter 1 Getting Started 1–3

Table 1–2 lists the components that make up the MICROLAB 500B Shipping Kit (shown as a box in Figure 1–1). Figure 1–2 shows these components.

Table 1–2 MICROLAB 500B Shipping Kit

Shipping Kit*

Part #

MICROLAB

510B

35792

MICROLAB

530B

35793

MICROLAB

540B

35794

Valve Part # Hand Probe Part

HV Valve

35825

Concorde

Probe 35529

Diluter

Valve

35844

Dispenser

Valve

35842

Concorde

Probe 35529

Dual Hand

Probe 35767

Small Parts Kit

Part #

35888 35887

Tubing Kit

Part #

2 items

*The MICROLAB 500C is standard with only the valve. All other parts must be ordered separately.

1–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figure 1–2 MICROLAB 500B Shipping Kit Components (items

shown are not to scale)

Small Parts Kit

Part # 35888

Concorde Probe

(Microlab 510B/511C or 530B/531C)

Part # 35529

Tubing Kit

Part # 35887

Dispenser Valve

Dual Hand Probe

Part # 35842

Part # 35767

(Microlab 540B/541C)

HV Valve

Part # 35825

(Microlab 510B/511C)

Diluter Valve Part # 35844

Chapter 1 Getting Started 1–5

Table 1–3 lists the components that make up the MICROLAB 500B Small Parts Kit (shown as a box in Figure 1–2). Figure 1–3 shows these components.

Table 1–3 Small Parts Kit #35888 (For All 500B Models)

Tubing Clips

Part #

230010

2 items

1 AMP Fuses

Part #

1524-01

2 items

Accessory

Holder

Part #

35783

1 item

Screws

Part #

16500

2 items

Figure 1–3 Small Parts Kit (items shown are not to scale)

(2) Tubing Clips

Part # 230010

Tubing Reducers

Part # 35770

Accessory

Holder

Part # 35783

Tubing

Reducers

Part #

35770

2 items

(2) Screws

Part # 16500

(2) Fuses

Part # 1524-01

1–6 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Table 1–4 lists the components that make up the MICROLAB 500 Tubing Kit (shown as a box in Figure 1–2). Figure 1–4 shows these components.

Table 1–4 Tubing Kit #35887 (For All 500B Models)

12 ga. x 650 mm

Fill Tubing (not

tapered)

Part #

18 ga. x 650 mm

Fill Tubing (not

tapered)

Part #

12 ga. x 900 mm

Dispense Tubing

(tapered)

Part #

18 ga. x 900 mm

Dispense Tubing

(tapered)

Part #

240000* 240010* 240360* 240130*

* M6 threaded hubs are used on all tubing.

Figure 1–4 Tubing Kit (items shown are not to scale)

12 ga. Fill Tubing

Part # 240000

12 ga. Dispense Tubing

Part # 240360

18 ga. Fill Tubing

Part # 240010

18 ga. Dispense Tubing

Part # 240130

For complete lists of syringes, accessories, and replacement parts for the MICROLAB 500, see Appendix C, Ordering Parts and Accessories for the MICROLAB 500.

Chapter 1 Getting Started 1–7

A Brief Introduction to the MICROLAB 500B

The MICROLAB 510B, 530B, and 540B systems each consist of three basic units. These units include:

• a drive unit

• a controller unit

• a hand probe This section briefly describes these units and the individual components that

comprise each unit. See Chapter 2, Installing the MICROLAB 500, for complete installation instructions; see Chapter 3, Using the MICROLAB 500, for complete usage instructions.

Drive Unit

The drive unit is the heart of each MICROLAB 500 system. The drive unit contains a precision drive motor, the syringe drive arms, the valve assembly, the power switches, and the connector receptacles. These features allow you to control other sub-assemblies, and together they create a very versatile and functional instrument.

Figure 1– 5 shows the front view of the MICROLAB 510B single syringe diluter/dispenser. In this figure, the controller unit rests on top of the drive unit and a syringe is attached to the syringe drive arm.

A Warning! This warning label appears on the front panel of the

ML500. It indicates that a pinch hazard exists when the syringe drive is moving.

1–8 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figure 1–5 Front View of the MICROLAB 510B Single Syringe

Diluter/Dispenser

Input

12 4 7

56 89 0

Run Stop

Hand Probe Connector Receptacle

Output

STEP

PRIME

Step/Prime Switch

Power Indicator Light

Power

POWER

On/Off Switch

Chapter 1 Getting Started 1–9

Figure 1–6 shows the front view of the MICROLAB 530B dual syringe diluter. In this figure, the controller unit rests on top of the drive unit and syringes are attached to the syringe drive arms.

Figure 1–6 Front View of the MICROLAB 530B Dual Syringe Diluter

12 4 7

56 89 0

Run Stop

Hand Probe Connector Receptacle

OutputInput

STEP

Step/Prime

PRIME

Switch Power

Indicator Light

Power

POWER

On/Off Switch

1–10 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figure 1–7 shows the front view of the MICROLAB 540B dual syringe dispenser. In this figure, the controller unit rests on top of the drive unit and syringes are attached to the syringe drive arms.

Figure 1–7 Front View of the MICROLAB 540B Dual Syringe

Dispenser

Input

Output

12 4 7

56 89 0

Run Stop

Hand Probe Connector Receptacle

Input

STEP

PRIME

Output

Step/Prime Switch

Power Indicator Light

Power

POWER

On/Off Switch

Chapter 1 Getting Started 1–11

Figure 1–8 shows the rear view of all units.

Figure 1–8 Rear View of the MICROLAB 500.

Hand Probe

Connector

Receptacle

LR-52122

RENO, NEVADA

BAUD

UNIT #

Baud Rate Switch

Protocol

Switch

Loopback Switch

TTL Port

RS-232 OUT

Receptacle

Controller Unit

Connector

Receptacle

(RS-232 IN)

Fuse Box

Power Cord

Connector

RS-232

OUT

PART

NO.

MANUFACTURED BY

TTL

SERIAL

NO.

WARNING: DISCONNECT SUPPLY BEFORE SERVICING. HIGH LEAKAGE CURRENT-ENSURE PROPER GROUNDING. AVERTISSEMENT: COUPER LALIMENTATION AVANT L ENTRETIEN ET DEPANNAGE.

COURANT DEFUITE ELEVE-FOURNIER UNE MISE A LA TERRE EFFICACE. WARNUNG: FUER SERVICEARBEITEN STROMZUFUHR UNTERBRECHEN. HOHER LECKSTROM-GUTER ERDUNGSANSCHLUSS GEWAEHRLEISTEN.

100-240 V 50-60 HZ 150 VA

FUSE: T 1A/250 V

LABEL NO. 10650

RS-232

Receptacle

1–12 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Power Cord Connector Receptacle

The power cord connector receptacle is located on the back of the drive unit; the power cord fits into this receptacle only one way. The receptacle accepts cords for either 115V or 220V without any adaptation of the drive unit. See Figure 1–8 for the location of the power cord connector receptacle.

Hand Probe or Footswitch Connector Receptacle

The hand probe connector receptacle is located on the upper right side of the drive unit. You can insert either a hand probe jack or a footswitch jack into this receptacle. See Figures 1–5 through 1–8 for the location of the hand probe connector receptacle.

Fuse Box

The fuse box is located at the top of the power cord connector receptacle. To gain access to the fuse box, you must first remove the power cord. See Figure 1–8 for the location of the fuse box.

Communications Settings

All communications switches and ports are located on the back of the drive unit beneath the serial number label. These items are listed here; refer to “Selecting Communications Options” in Chapter 2 for complete information about setting and using these receptacles and switches.

• The RS/232 IN receptacle handles incoming communications.

• The RS/232 OUT receptacle handles outgoing communications.

• The TTL port allows peripheral devices to attach to the drive unit.

• The Loopback switch allows the drive unit to daisy-chain to other units.

• The Baud Rate switch controls the speed at which the drive unit communicates with other devices.

• The Protocol switch determines the hardware address and protocol of the drive unit.

Chapter 1 Getting Started 1–13

Power On/Off Switch and Power Indicator Light

The Power On/Off switch and the Power Indicator light are located on the front of the drive unit in the lower right-hand corner. See Figures 1–5 through 1–7 for the locations of the switch and the indicator light.

The Power On/Off switch is a two-position rocker switch.

• To power-on the MICROLAB 500, press the upper half of the switch. The system beeps twice when it is powered on.

• To power-off the MICROLAB 500, press the lower half of the switch.

The Power Indicator light is a small green LED located directly above the Power On/Off switch. It is lit when the unit is powered on.

Step/Prime Switch

The Step/Prime switch is located on the front of the drive unit in

C / D

The Step/Prime switch is a three-position rocker switch.

the lower right-hand corner, directly above the power indicator light. See Figures 1–5 through 1–7.

• When the Step/Prime switch is in the middle position, the switch is inactive.

1–14 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

• When you press the lower part of the Step/Prime switch , the

Use the Step mode to move the syringe drive arm down and away from the home position before installing or removing syringes.

Valve Assembly

The valve assembly controls the flow of liquid through the fluid path. The 510B/511C, 530B/531C, and 540B/541C models each use a different valve assembly; you must attach the assembly to the drive unit. See Figures 1–5 through 1–7 for the location of the valve assembly. See “Installing the Valve Assembly” in Chapter 2 for installation instructions.

ML500 goes into prime mode. When in prime mode, the syringe drive arms automatically move up and down, opening the valve ports, and moving fluid through the system. The ML500 stays in prime mode until you press the upper or the middle part of the Step/Prime switch. The syringe will return to home position.

• When you press the upper part of the Step/Prime switch , the ML500 goes into Step mode. When in Step mode, the drive arms move only as long as you press the Step/Prime switch. When you release the switch, movement stops.

Syringe Drive Arms

You attach syringes to the MICROLAB ’s syringe drive arms. The syringe drive arms are engineered to drive Hamilton precision syringes with high-resolution stepper motors; the motors and drive arms are connected by a precision lead screw. See “Selecting, Installing, and Removing Syringes” in Chapter 2 for syringe installation instructions.

Chapter 1 Getting Started 1–15

Controller Unit (for MICROLAB 500B Models)

The controller unit is a portable device that connects to the syringe drive unit via the controller connector cord. Use the controller unit to program and store methods and send information and instructions to the drive unit. For ease of use and for everyday storage, the controller unit fits on top of the drive unit. Figure 1–9 shows the controller unit.

The display screen and controls and are located on the unit:

• display screen—The display screen is located at the top of the unit. It shows the status of the instrument and provides information about current methods.

• function keys—The function keys are located directly beneath the display screen. Use these keys to perform the various operations shown on the display screen.

• numeric keypad—The numeric keypad is located in the lower left corner of the controller unit. Use the numeric keypad characters to enter numeric data into the methods that you create or run.

• arrow keys—The arrow keys are located on the right side of the controller unit. Use these keys to move from field to field on the display screen.

• Run/Stop key—The Run/Stop key is located in the bottom right corner of the controller unit. This key generates a command signal that activates the next step of an operation.

See Chapter 3, Programming and Using the MICROLAB 500 System, for complete instructions about using the controller unit’s various keys.

✽ Note: 500C models may be operated with the controller unit; However,

the controller is not included in the standard 500C package.

1–16 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

✽

Figure 1–9 The Controller Unit

Display

Screen

Numeric

Keypad

Function Keys

Arrows

Run Stop

Keys

Run/Stop Key

Chapter 1 Getting Started 1–17

Pipettors/Probes

Each MICROLAB 500B system comes with a remote push-button, hand-held pipettor/probe. The standard accessory probes include:

• the Concorde Push-button Hand Pipettor/Probe (ships with MICROLAB 510B and 530B; must be ordered separately for 500C models)

• the Dual Push-button Hand Pipettor/Probe (ships with MICROLAB 540B; must order separately for the 541C model)

Additionally, three other optional probes may be ordered separately for use with any MICROLAB 500 system. These probes include:

• the Disposable Tip Push-button Hand Pipettor/Probe

• the Luer Lock Tip Push-button Pipette Hand Pipettor/Probe

• the Viscous Sample Push-button Hand Pipettor/Probe

Concorde Push-button Hand Pipettor/Probe

The Concorde Push-button Hand Pipettor/Probe is a single-dispense pipette probe that features a slim, comfortable design. Use this probe with MICROLAB 501 and 503 systems. The Concorde probe attaches directly to a sampling syringe or to the valve assembly (output port). This probe accommodates both 12- or 18-gauge tubing and features adjustable extension lengths beyond the probe tip. See Figure 1–10.

Dual Push-button Hand Pipettor/Probe

The Dual Push-button Hand Pipette Probe is a dual-dispense pipette that features a pistol grip design with push-button actuator. Use this probe with MICROLAB 504 systems. This probe accommodates both 12- and 18-gauge tapered tubing lines with independent extensions. You can extend the tubing out the end of the probe to the length that is convenient for your application; either the right-side or the left-side tubing can be extended to pick up sample. Figure 1–10 shows the Dual Push-button Hand Pipette Probe.

1–18 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Disposable Tip Push-button Hand Pipettor/Probes

The Disposable Tip Push-button Hand Pipette Probe is a single-dispense tube pipette that features disposable tips and push-button tip ejection. The Disposable Tip Push-button Hand Pipette Pipettor/Probe is an optional accessory. Use this probe when sample-to-sample carryover is a concern. See Figure 1–10.

Figure 1–10 shows the Concorde, the Disposable Tip, and the Dual Hand Pipettor/Probe.

Figure 1–10 The Concorde, the Disposable Tip, and the Dual Hand

Pipettor/Probes

Concorde

Pipettor

Probe

Disposable Tip

Pipettor

Probe

Dual Hand

Pipettor

Probe

Luer Lock Tip Push-button Pipette Hand Pipettor/Probe

The Luer Lock Tip Push-button Pipette Hand Pipettor/Probe is an optional accessory that dispenses liquids through needles of different lengths and gauges. This probe is useful for controlling very small dispense volumes with a high degree of accuracy. It is also ideal for administering injections to small animals and for piercing septa.

Chapter 1 Getting Started 1–19

Viscous Sample Push-button Hand Pipette Pipettor/Probe

The Viscous Sample Push-button Hand Pipettor/Probe is an optional accessory. This single-dispense tube pipette is designed to accommodate highly viscous samples, such as motor oil. This accessory features a disposable 5 mL tip that eliminates sample carryover. Air displacement ensures accurate dilutions.

Footswitch

In addition to the hand-activated probes, a footswitch is also available. The footswitch allows you to operate the MICROLAB 500 via a foot-activated control pedal. This is useful when you need to keep both hands free, as when doing handsampling.

Refer to Appendix C for information about ordering the footswitch or any other optional accessories.

Probe Button Functions

The probe button (on any model of hand probe) serves basically the same function as the Run/Stop key on the controller unit. However, you cannot use the probe button to pause an operation or to reinitialize the system after an error occurs.

1–20 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Chapter 2 Installing the MICROLAB 500

System

This chapter contains information about installing the MICROLAB 500 system, including the following topics:

• an overview of installation procedures

• selecting an installation location

• installing the accessory holder

• determining communications settings

• installing electrical connections

• installing the valve assembly

• selecting, installing , and removing syringes

• selecting and installing tubing

▲ Important Be sure to read the instructions in this chapter before

installing your MICROLAB system.

• Never install or use syringes incorrectly. Incorrect use may result in damage to the syringes. See “Selecting, Installing, and Removing Syringes” later in this chapter for more information.

• Never over-tighten tubing. Over-tightening may result in damage to the valves or tubing. See “Selecting and Installing Tubing” later in this chapter for more information.

• Call Hamilton Company’s Technical/Customer Service Department at (800) 648–5950 (in the United States and Canada) if you have questions about installing your

MICROLAB 500 system.

▲

2–1

Figure 2–1 Overview of Installation Procedures

Unpack the instrument;

check all parts against packing list

Select an installation location

Install the accessory holder

Determine the communications settings

Plug in electrical connections:

• power cord • hand probe

• controller unit cord

• alternate communications cords

Install the valve assembly

Select

syringe

sizes

Install syringe(s)

Select tubing

sizes

Install the fill tubing

Install the tubing clip

Install the dispense tubing

Run the dispense tubing through the hand probe

Fill out and return warranty card

Installation

is complete

2–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Overview of Installation Procedures

Figure 2–1 provides an overview of the MICROLAB 500B installation procedures. These procedures are described in detail in this chapter.

Selecting a Location

Install your MICROLAB 500 system in a clean, dry, level area away from hazardous fumes. Leave space around the unit for ventilation; three inches is sufficient.

Installing the Accessory Holder

All MICROLAB systems come with an accessory holder. You can mount the accessory holder on either the right or the left side of the instrument. Generally, the accessory holder is mounted on the right side and is used to hold a hand probe when the probe is not in use. To install the accessory holder, follow these steps:

1. Locate the threaded holes on either the right or the left side of the instrument.

2. Use a hex wrench and screws (screws are provided) to attach the holder.

Chapter 2 Installing the MICROLAB 500 System 2–3

Selecting Communications Options

To select communications options, you must first decide how you will use the instrument. That is, will it be a used as standalone device or will it be on a daisy chain? Based on its use, select the appropriate communications settings. See Figure 2–2 for the location of the communication switches and ports. Table 2–1 describes the purposes of these switches and ports.

To change a switch’s position, power the instrument off. Then use a screwdriver to change the switch position. The small bump on the switch indicates the selected position. After you change a switch’s position, power the instrument back on again.

Table 2–1 Communications Switches and Ports

Icon Item Purpose Possible Settings and Uses

TTL

RS-232 IN receptacle

RS-232 OUT receptacle

TTL port allows peripheral

controller or RS232 three possible uses:

handles outgoing communications

devices to attach to the drive unit

1. controller via coiled cord

2. connects to the communications port on a PC

3. if on a daisy chain, connects to the RS232 OUT port of the previous unit on the chain

two possible uses:

1. if not on a daisy chain, is left open

2. if on a daisy chain, connects to the RS232 IN port of the next chained device; is left open if it is the last unit on the chain

attach digitally-controlled devices such as pumps, monitors, etc.; is left open if no peripherals are attached

2–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Table 2–1 Communications Switches and Ports

Icon Item Purpose Possible Settings and Uses

(continued)

BAUD

UNIT #

Loopback switch

Baud Rate switch

Protocol switch

allows the drive unit to daisy-chain to other units

controls the speed at which the drive unit communicates with other devices

baud rate on the controller unit must match the baud rate on the drive unit at startup time; the default is 9600

determines the hardware address and protocol of the drive unit; the default is Protocol 1 (position 0)

two possibilities:

1. UP: indicates the unit is a standalone unit OR the last item on a daisy chain

2. DOWN: indicates the unit is a member of a daisy chain, in any position except the last position

six possibilities: 0 = 1200

1 = 2400 2 = 4800 3 = 9600 (system default value) 4 = 19200 5 = 38400 6 = Unused 7 = Unused 8 = Unused 9 = Unused

two possibilities: 0 = Protocol 1/RNO+Syntax

(system default value)

• maximum items on a chain is 16

1–F = DIN Protocol

• if the drive unit is a member of a daisy chain then each member of the chain must have its own unique address

• hardware address may/may not be out of sequence

• maximum items on a chain is 15

Chapter 2 Installing the MICROLAB 500 System 2–5

Refer to Appendix E, RS-232 Communications with Manual ASCII Commands, and Appendix F, Protocols, for more detailed information about MICROLAB 500 system communications. Refer to Chapter 3, Programming and Using the MICROLAB 500 System, for more information about baud rate settings.

Installing Electrical Connections

A Warning! Always make sure that the instrument is powered off

before installing or removing any electrical connections.

Refer to Figure 2–2 and follow these steps to install electrical connections:

1. Plug the power cord into the power connector receptacle on the back of the drive unit. The power cord fits into the receptacle only one way.

The power connector receptacle accepts cords for either 115V or 220V.

2. Plug the hand probe (or foot pedal) jack into the probe connector receptacle. The probe connector receptacle is located on the upper right side of the drive unit.

3. The controller connector receptacle is located on the back of the drive unit below the serial number plate.

If you will be using the controller unit to communicate to the base unit, plug the controller cord into the receptacle just as you plug a telephone cord into a telephone outlet. Pinch the plastic locking device on the cord and insert it into the connector receptacle. Then release the plastic locking device, firmly attaching the cord.

4. If you will be using the drive unit to communicate to other devices, attach the appropriate cords.

– To use the drive unit on a daisy chain, do not install the controller unit.

Instead, install the interconnect cord (Part # 35833) and connect it to the RS-232 IN receptacle of the next device on the daisy chain. Leave the RS-232 OUT receptacle open if the drive unit is the last unit on the daisy chain.

2–6 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

For more information about possible communications options, refer to Table 2–1, Communications Switches and Ports.

Figure 2–2 Installing Electrical Connections

Hand Probe

Connector

Receptacle

RS-232 OUT

Receptacle

Controller Unit

Connector

Receptacle

(RS-232 IN)

Fuse Box

Power Cord

Connector

Receptacle

RS-232

OUT

PART

NO.

MANUFACTURED BY

TTL

SERIAL

NO.

WARNING: DISCONNECT SUPPLY BEFORE SERVICING. HIGH LEAKAGE CURRENT-ENSURE PROPER GROUNDING. AVERTISSEMENT: COUPER LALIMENTATION AVANT L ENTRETIEN ET DEPANNAGE.

COURANT DEFUITE ELEVE-FOURNIER UNE MISE A LA TERRE EFFICACE. WARNUNG: FUER SERVICEARBEITEN STROMZUFUHR UNTERBRECHEN. HOHER LECKSTROM-GUTER ERDUNGSANSCHLUSS GEWAEHRLEISTEN.

100-240 V 50-60 HZ 150 VA

FUSE: T 1A/250 V

LABEL NO. 10650

RS-232

LR-52122

RENO, NEVADA

BAUD

Accessory Holder

UNIT #

Baud Rate Switch

Protocol

Switch

Loopback Switch

TTL Port

Chapter 2 Installing the MICROLAB 500 System 2–7

Installing Valve Assemblies

There are three types of valve assemblies for the MICROLAB 500 series. Instructions for installing valve assemblies follow.

Installing the Valve Assembly on the MICROLAB 510B/511C

The MICROLAB 510B/511C uses a single active valve assembly. To install the valve assembly, follow these steps:

1. Pick up the valve assembly. Make sure the PTFE luer fitting that attaches to the syringe is on the bottom of the assembly.

2. Holding the valve assembly, align the valve stem opening with the valve motor drive port on the drive unit. Press the valve assembly into place.

3. Push down on the valve lever to lock the valve assembly to the drive unit.

Figure 2–3 Installing a Valve Assembly on the MICROLAB 510B/511C

Valve Stem

Valve

PTFE

Luer Fitting

Valve Motor

Drive Port

Valve Lever

Lock

2–8 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Installing Valve Assemblies on the MICROLAB 530B/531C and 540B/541C

Although the valve assemblies for the 530B/531C and 540B/541C units are different from one another, their installation procedures are the same. To install a valve assembly on either the MICROLAB 530B or 540B, follow these steps:

1. Pick up the valve assembly. Make sure the PTFE luer fittings that attach to the syringes are on the bottom of the assembly.

2. Insert the valve assembly into the valve motor drive ports and press the valve assembly into place.

3. Tighten the thumbscrew until it is finger-tight to secure the valve assembly to the drive unit.

Figure 2–4 Installing a Valve Assembly on the MICROLAB 530B/531C and

540B/541C

Valve Motor

Drive Ports

Valve Stem

Valve

Thumbscrew

Chapter 2 Installing the MICROLAB 500 System 2–9

Selecting, Installing, and Removing Syringes

Before you install syringes on any MICROLAB instrument, you must first decide what syringes to use. Use Tables 2–2 and 2– 3 to select the best syringe for your application. See Figures 2–5 through 2–7 for examples of different syringe types.

• The MICROLAB 510B/511C uses one dispenser/diluent-type syringe in the left position (TLL-type or TLLX-type syringes).

• The MICROLAB 540B/541C uses two dispenser/diluent-type syringes—one each in the left and right positions (TLL or TLLX).

• The MICROLAB 530B/531C uses one reagent/diluent syringe in the left position and one sample syringe in the right position (DX, TLL, or TLLX).

• As a general rule, to ensure high accuracy and precision, try to use 10% or more of a syringe’s total volume. Consider using a different size syringe if your application calls for driving a syringe less than 10% of its total volume, and high accuracy and precision are required.

Table 2–2 Reagent/Diluent Syringes (TLL- and TLLX-types)

Syringe Sizes Model Number Part Number Optimal Ranges

(µL)

25 µL 1702 TLLX 80222 2.5–25

50 µL 1705 TLLX 80922 5–50 100 µL 1710 TLLX 81022 10–100 250 µL 1725 TLLX 81122 25–250 500 µL 1750 TLLX 81222 50–500

1 m L 1001 TLLX 81323 100–1000

2.5 mL 1002 TLL 81420 250–2500

5 m L 1005 TLL 81520 500–5000 10 m L 1010 TLL 81620 1000–10,000 25 m L 1025 TLL 82521 2500–25,000

2–10 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Table 2–3 Sample Syringes (DX-type)

Syringe Sizes Model Number Part Number Optimal Ranges

(µL)

25 µL 1702 DX 80226 2.5–25 50 µL 1705 DX 80926 5–50

100 µL 1710 DX 81026 10–100 250 µL 1725 DX 81126 25–250 500 µL 1750 DX 81226 50–500

1 m L 1001 DX 81326 100–1000

See Appendix C for information regarding replacement parts for TLL, TLLX, and DX syringes.

Figure 2–5 The TLL-type Dispenser/Diluent Syringe

Figure 2–6 The TLLX-type Dispenser/Diluent Syringe

Figure 2–7 The DX-type Sample Syringe

Chapter 2 Installing the MICROLAB 500 System 2–11

Preparing Syringes for Installation

When you unpack a new syringe, notice that the syringe plunger is packed separately from the syringe barrel. You must condition the plunger tip before inserting the plunger into the barrel.

▲ Important Before using a new syringe for the first time, you

must condition the syringe’s PTFE plunger tip and glass barrel. To condition the tip and barrel, wet the plunger tip with distilled water or a solvent. (Medicaltype silicone lubricants may be used to extend the lives of PTFE plunger tips. However, silicone may contaminate the process fluid.) Do NOT use viscous oils to lubricate plunger tips.

After wetting the plunger tip, insert the plunger into the glass barrel. Move the plunger in and out of the glass barrel approximately 10 times. Apply steady

and even pressure; avoid twisting movements.

▲

Installing Syringes

Follow these steps to install syringes on any MICROLAB 500 system.

1. Condition all syringes before installation by following the procedure described in “Preparing Syringes for Installation.”

2. Power on the MICROLAB system using the Power On/Off switch.

3. Press and hold the Step/Prime switch in the Step position, and move the syringe drive arm down from the Home position. Release the switch when the drive arm is about half-way between Home and the Down position.

2–12 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

4. Fasten the thumbscrew on the drive arm to the bottom of the syringe plunger. To fasten the thumbscrew, hold the plunger and screw the thumbscrew into the threaded fitting at the bottom of the syringe.

5. Pull the glass barrel up straight to the threaded female luer fitting that extends down from the bottom of the valve. Insert the male luer fitting into the valve fitting and turn the glass barrel clockwise until it is “finger-tight.”

See Figure 2–8, Installing a Syringe, for an illustration of these steps.

Figure 2–8 Installing a Syringe

Then, mount the Luer

First, mount the

Lock.

Thumbscrew.

Chapter 2 Installing the MICROLAB 500 System 2–13

▲ Important Always tighten syringes so they are “finger-tight.”

Syringes that are over- or under-tightened can cause problems for your MICROLAB 500 system.

• Syringes that are over-tightened may cause leaks or may damage the valve.

• Syringes that are under-tightened may cause leaks.

• Syringes that are not screwed on straight may leak and cause lateral strain on the syringe luer fitting

as it connects to the bottom of the valve.

▲

Removing Syringes

To remove syringes, reverse the installation procedure.

1. Power on the system using the Power On/Off switch.

2. Use the Step/Prime switch to move the syringe drive arm down from the Home position. Release the switch when the drive arm is about half way between Home and the Down position.

▲ Important Before you use the Prime function, direct the probe

toward a liquid waste container or reservoir since initialization may expel any fluid remaining in the

syringes.

3. Release the glass barrel by turning it counterclockwise.

4. Unfasten the thumbscrew on the drive arm at the bottom of the syringe plunger. To unfasten the thumbscrew, hold the plunger and unscrew the thumbscrew from the threaded fitting at the bottom of the syringe.

Refer to Figure 2–9, Removing a Syringe, for an illustration of the removal procedure.

2–14 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

▲

Figure 2–9 Removing a Syringe

First, remove the

Luer Lock

Then, remove the

Thumbscrew.

Warning! Avoid the risk of injury or infection! Use extreme

caution when removing cracked or splintered syringes. Always wear thick gloves and protective

eye wear when replacing syringes.

Chapter 2 Installing the MICROLAB 500 System 2–15

Selecting and Installing Tubing

This section provides information about selecting and installing tubing on all MICROLAB 500 systems.

Selecting Tubing

Each MICROLAB system includes two gauges of fill and dispense tubing: 12- and 18gauge. Fill tubing has a 90˚ cut on the end opposite the fitting; dispense tubing has a tapered end opposite the fitting.

When selecting tubing, consider the volume and the viscosity of the liquid you plan to pipette. Refer to Table 2–3, Tubing Selection Guide, for help in selecting tubing.

Table 2–4 Tubing Selection Guide

Syringe

Size

Tubing Gauge for

Standard Aqueous

Solutions

Tubing Gauge for

Viscous and

Foaming Liquids

25 µL18 18

50 µL18 18 100 µL18 18 250 µL18 18 500 µL18 18

1 mL 18 18

2.5 mL 18 12

5 mL 12 12 10 mL 12 12 25 mL 12 12

2–16 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

✱ Note: If you have a MICROLAB 530B/531C diluter and plan to use

syringes of two different sizes, choose tubing to accommodate the syringe with the larger volume. ✱

See Appendix C for information about ordering extra tubing.

Installing Tubing

1. Before installing tubing lines, wet the tubing fittings.

2. Make sure the tubing lines do not contain crimps or blockages. Crimps or blockages can cause leaks or can cause the system to overload. If tubing is crimped, replace it with new tubing.

▲ Important Fasten tubing so it is finger-tight and snug enough to

prevent leaks. Avoid using tools to tighten tubing. Excessive tightening may damage tubing, valve

fittings, or flanges.

3. Install the fill tubing. You can differentiate fill tubing and dispense tubing since dispense tubing has a tapered end.

▲

– For all MICROLAB 500 models, install the fill tubing on the left, or input

side of the valve. Thread the tubing fitting into the left valve port, and screw it in so it is finger-tight.

– For the MICROLAB 540B/541C only, install a second fill tubing line on the

right side of the valve. Thread the tubing fitting into the right valve port,

and screw it in so it is finger-tight, just as you did on the right side.

4. Once the fill tubing is installed, attach a tubing clip to it. Slip the tubing clip over the end of the tubing, then attach the clip to the side of the reservoir.

Chapter 2 Installing the MICROLAB 500 System 2–17

5. Install the dispense tubing. You can recognize dispense tubing by its tapered end. – If you are using a MICROLAB 510B/511C, install the dispense tubing on the

right side of the valve. Thread the tubing fitting into the right valve port and screw it in so it is finger-tight. See Figure 2–10 for an illustration of the valve and tubing connectors.

Figure 2–10 MICROLAB 510B/511C Valve and Tubing Connections

Input Side

(Port)

Output Side

(Port)

– If you are using a MICROLAB 530B/531C with a D syringe, install the

dispense tubing on the side port of the D syringe. See Figure 2–11. When using a TLL syringe as a sampling syringe, install dispense tubing directly onto the valve assembly. To do this, remove the valve plug and replace the plug with dispense tubing. See Figure 2–12.

2–18 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Figure 2–11 MICROLAB 530B/531C Valve with a D Configuration Syringe

Insert Valve Plug First

Input Side

(Port)

Output Side

(Port)

Figure 2–12 MICROLAB 530B/531C Valve with Two TLLX or TLL Syringes

Remove Valve Plug First

Input Side

(Port)

Output Side

(Port)

Chapter 2 Installing the MICROLAB 500 System 2–19

– If you are using a MICROLAB 540B/541C, install a tapered dispense tubing

line into each of the valve’s front ports. The front ports are located on the bottom of the valve between the two syringes. Thread the tubing fittings into the front ports, and screw the fittings in so they are finger-tight. See Figure 2–13.

Figure 2–13 MICROLAB 540B/541C Valve and Tubing Connectors

Input Side

(Port)

Input Side

(Port)

Output Ports

✽ Note: It is possible to use the 540B/541C dual dispenser as a single

dispenser. When programming, designate the instrument as a single dispenser.

6. Install the dispense tubing to the hand probe. – If you are using the MICROLAB 510B or the MICROLAB 530B, the

Concorde Probe comes as standard equipment. This probe uses a single dispense tube. To attach the dispense tubing into the probe, loosen the knurled screw at the upper end of the probe. Slide the tapered end of the dispense tube through the probe so that the tubing passes out the end of the probe. When enough tubing for you to work with extends from the probe, tighten the knurled screw to secure the tubing line.

2–20 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

✽

– If you are using the MICROLAB 540B, the Dual Hand Probe comes as

standard equipment. This probe is designed for both 18- and 12-gauge tubing. Use the tubing reducer (Part # 35770) that is provided with the dual hand probe if you wish to use 18-gauge tubing. See Figure 2–14.

Figure 2–14 Using the Tubing Reducer with the Dual Hand Probe

7. Fill out the warranty card and return it to Hamilton Company.

8. You are now ready to use your MICROLAB 500 system.

Chapter 2 Installing the MICROLAB 500 System 2–21

Chapter 3 Programming and Using the

MICROLAB 500B System

This chapter contains information about the following topics:

• an overview of the MICROLAB 500 functions

• using the controller unit keys

• accessing the Main menu

• priming and checking the system

• creating methods

• running methods

• using the Utility menu functions For information on controlling 500C systems, refer to Appendixes

E and F.

Figure 3–1 Using the MICROLAB 500B

Power up the instrument

Prime the instrument

Select a function

from the Main menu

Follow the on-screen instructions

Finished using the

instrument?

Yes

When finished, flush the fluid line

and turn the instrument off

Select another function

3–1

Figure 3–2 provides an overview of the functions available using the MICROLAB 500B. These functions are described in detail later in this chapter.

Figure 3–2 Overview of MICROLAB 500B Functions

Power up/

startup

screen

Main

Run existing method

Select

method

Run

Pipette Dilute

Create

new

method

Name

Instrument

configuration:

single/dual

Select

method type

to create

Set:

• Gap

• Speed

• # of Syringes

Wash?

Edit existing method

Select

method

Edit

Save and

return to

Main menu

Aliquot

configuration:

Dispense

Manual

dilute

Instrument single/dual

Run Run

Titrate

Serial

Manual

dispense

Instrument

configuration:

single/dual

Custom

Write

method

UtilitiesPrime

See

Utilities menu

flowchart

Save and

return to

Main menu

3–2 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Using the Controller Unit

Use the controller unit to create and run methods, to enter and change data, and to perform utility functions. Figure 3–3 shows the controller unit.

Figure 3–3 The Controller Unit

Display

Screen

Function Keys

Arrows Keys

Run/Stop Key

Numeric

Keypad

Run Stop

Using the Arrow Keys

The arrow keys are located on right side of the controller unit, between the function keys and the Run/Stop key. Use the arrow keys to move from one data entry field to another on the display screen; each key moves you in the direction that it points. The current field is highlighted in reverse video on the display screen. In this manual, items are highlighted with boldface text.

Chapter 3 Using the MICROLAB 500 System 3–3

Using the Numeric Keypad

The numeric keypad is located in the lower left corner of the

3 56 89

controller unit. Use the numeric keypad to enter numeric data when using the edit mode (see “Editing Conventions” for information about the edit mode).

Using the Run/Stop Key

The Run/Stop key is located in the bottom right corner of the

controller unit. This key generates a command signal that activates the next step or pauses the unit during a method. You can also generate a start signal by pressing the button on a hand probe or by using a footswitch.

Using the Function Keys

The function keys are located directly below the display screen. Use these keys to perform specific actions, based on the requirements of the current screen. To perform an action, press the function key located beneath the action. Not all actions are available from all screens.

Table 3–1 Function Key Actions

SELECT selects the highlighted element on the screen so it can be edited or

acted upon in some manner

ESCAPE returns you to the previous screen without saving any changes; also

abandons the current edit mode process

ACCEPT accepts the displayed screen with any changes CONFIRM verifies that you want to save your changes or that you want to

perform an action

DELETE deletes the highlighted element on the screen ENTER accepts the underlined value entered while in edit mode MODE allows you to choose the next operation to be performed in a manual

method

3–4 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Editing Conventions

To use the edit mode, press the appropriate arrow key to move to the desired screen location. Then press the SELECT function key; you must always press SELECT before you can change the value of any highlighted item. For example, to enter a size for a syringe, use an arrow key to move to and highlight the syringe size field.

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 1000.0 Right Syringe size (uL): 1000.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

When you press SELECT, notice that the highlighting changes to underlining. Now you can edit the underlined value; instructions for data entry appear near the bottom of the screen. Use the arrow keys (or in some cases, the numeric keypad numbers), to increase or decrease the underlined value. When you are satisfied with your entry, press ENTER.

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 2000.0 Right Syringe size (uL): 1000.0

Use arrow keys to change syringe size

ENTER ESCAPE

Chapter 3 Using the MICROLAB 500 System 3–5

Pressing ENTER returns you to the previous screen, keeping the data entry change you just made. When you are finished editing, press ACCEPT to use the values displayed in all fields on the screen.

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 2000.0 Right Syringe size (uL): 1000.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

Powering on the MICROLAB 500

To power on the MICROLAB 500, press the Power On/Off switch located on the front panel of the drive unit. When the instrument powers up, it performs an electronic self-test and beeps twice. The first time that you start up the instrument, you see the following screen:

HAMILTBN

MICROLAB 500 Series

version #

3–6 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Next, the “Change default language” screen appears:

Change default language

English Francais Deutsch Português Español Clear default language

SELECT menu item

SELECT

Select a default language. The language that you select will become the system default, and all future screens will appear in that language. You will not be prompted again for a default language at startup. See “Changing the Default Language” later in this chapter for more information.

The Main Menu

After you select a default language, the Main menu appears:

MICROLAB 500 main menu

Run an existing method

Create a new method

Edit an existing method Manual dilution Manual dispense Prime the fluid path Utilities menu

SELECT menu item

SELECT

Use the arrow keys to highlight the desired function. Then press SELECT. Information about each of the Main menu functions follows in this chapter.

Chapter 3 Using the MICROLAB 500 System 3–7

Priming the MICROLAB 500

This section describes how to set up and prime the MICROLAB 500.

Preparing to Prime

Prime the MICROLAB 500 system before using it for the first time, at the start of a work day, and between fluid changes. Run fluid through the tubing lines to ensure that there are no bubbles or air gaps in the tubing. Check the fluid path for leaks and clean the fluid path, if necessary.

To prime the MICROLAB 500 and check the fluid path for leaks, follow these steps:

1. Fill a reservoir with deionized water. Place the end of the inlet tubing in the reservoir. Use the tubing clip to attach the tubing to the side of the reservoir.

2. Place the hand probe over the same reservoir or over a waste container.

3. Push the Prime switch on the drive unit OR select the Prime function from the main menu. See the next section, Using the Prime function from the Main menu, for more information.

4. Run the instrument in Prime mode until water flows through the entire system.

5. If the system is airtight, the fluid path will contain a solid column of water. – If you see air bubbles in the fluid path, the system is not airtight. Adjust

the tubing fittings or syringes. Check that all parts are snug and fingertight.

– If you do not see air bubbles in the fluid path, the system is airtight.

3–8 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

6. If you do not see air bubbles in the fluid path, but small bubbles appear on the

PTFE surface of the syringe, clean the internal fluid path with a mild syringe cleaner. Rinse with deionized water to rid the system of cleaning fluid. To clean the fluid path, follow the steps in Chapter 4, Caring for the MICROLAB 500.

7. After cleaning, reprime with deionized water to rinse the fluid path. Continue priming until you do not see any bubbles.

8. Remove the inlet tubing from the reservoir and prime air through the system. Priming air through the system clears the fluid path of the deionized water that you used as a rinse.

9. When the fluid path is free of deionized water, place the inlet tubing into a reservoir of the liquid you wish to dispense or use as a diluent. Prime this fluid through the system.

✱ Note: When priming reagent after a deionized water rinse,

dispense the first shot into a waste container, since it will contain a certain amount of the water droplets that remained in the tubing

line. ✱

10. Your MICROLAB 500 system is clean and ready for use.

▲ Important Do not operate either side dry, as this may cause

excessive wear on the syringes and valves.

Chapter 3 Using the MICROLAB 500 System 3–9

▲

Figure 3–4 shows the required steps for priming the instrument.

Figure 3–4 Priming the MICROLAB 500

select the Prime function from the Main menu

Press the Prime Switch OR

and start priming the instrument

Yes

1st time use?

Clean the

fluid path

Check the fluid path for leaks

Any leaks in

the fluid path?

Yes

Adjust tubing,

fittings, and

syringes

Any bubbles

on the plunger

tip?

Yes

Clean the

fluid path

Halt the priming cycle

Finished

using the

instrument?

Select another function

Yes

When finished, flush the fluid line

and turn the instrument off

3–10 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Using the Prime Function from the Main Menu

1. From the Main menu, SELECT the “Prime the fluid path” function. The following screen appears:

Drive unit initialization

Any fluid in the syringes on the drive unit will now be purged. Please ensure the probe is directed towards a waste area.

CONFIRM to continue, ESCAPE to exit

ESCAPE CONFIRM

▲ Important When you select the Prime method, the instrument

alerts you that the fluid in the syringes is about to be purged. Be sure to place the probe over a fluid waste area or a reservoir before pressing CONFIRM to

continue the priming function.

2. Press CONFIRM to continue the priming process.

3. The following screen appears:

▲

Prime the fluid path

Full Cycles: 10 Syringe prime speed: 4

SELECT item to edit-RUN to start

SELECT ESCAPE

– Enter the desired number of priming cycles; 10 is the default. – Enter the prime speed. Speed must be in whole seconds, from 1 to 250. – Press the Run/Stop key to start the priming cycle.

Chapter 3 Using the MICROLAB 500 System 3–11

4. The instrument displays the status of the priming cycle.

Prime the fluid path

Priming. . .

3 of 10 strokes completed.

STOP to pause

– Press the Run/Stop key to pause the priming cycle. If you pause the priming

cycle, you see the following screen:

Prime the fluid path

Priming. . .

3 of 10 strokes completed.

RUN to continue, ESCAPE to exit

ESCAPE

– Press the Run/Stop key once again to resume the cycle or press ESCAPE to

exit the cycle.

5. When the cycle is complete, the instrument displays a message stating that the prime is complete. Press any key to return to the main menu.

Prime the fluid path

Priming. . .

10 of 10 strokes completed. Prime complete

Press any key to continue

3–12 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Creating a New Method

Use this function to create and save a new method in the method storage area. You can create and save up to 21 methods. To create a new method, follow these steps:

1. From the main menu, use the arrow keys to highlight the “Create a new method” function, then press SELECT.

2. The instrument displays the method storage area with an unused method location already highlighted. Unused method locations are denoted by the term “<UNUSED>”. If desired, use the arrow keys to move to another location. Press SELECT to use a highlighted location or press ESCAPE to return to the main menu without creating a new method.

Create a new method

SELECT an UNUSED method

SELECT ESCAPE

3. If you press SELECT, you access the following screen:

Create a new method

1 2 3 4 5 6 7 8 9 Q W E R T Y U I O P

A S D F G H J K L

Z X C V B N M

SPACE

Name:__________

SELECT next letter-ACCEPT if complete

SELECT ESCAPE ACCEPT DELETE

Chapter 3 Using the MICROLAB 500 System 3–13

Use the arrow keys to spell out the new method’s name, using up to 10 characters.

– To accept a character, press SELECT. – To erase a character, press DELETE. – Press ESCAPE to return to the main menu without creating a new method. – Press ACCEPT when you are finished naming the new method.

4. Next, the instrument prompts you for the configuration you are using. Use the arrow keys to highlight either a one- or a two-syringe instrument configuration.

Instrument configuration

One syringe instrument

Two syringe instrument

SELECT menu item

SELECT ESCAPE

– Press SELECT to accept the highlighted instrument configuration. – Press ESCAPE to return to the previous screen without selecting an

instrument configuration.

5. Once you select an instrument configuration, you are prompted to enter syringe size(s). For example, in the following screen the user has selected a two-syringe instrument. The system supplies default syringe sizes.

3–14 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 1000.0 Right Syringe size (uL): 1000.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

6. You can use the default values that are displayed, or you can enter new sizes. To enter a new size, use the arrow keys to highlight the appropriate syringe, then press SELECT. Notice that the highlighting changes to an underline, as shown in the following screen:

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 2500.0 Right Syringe size (uL): 1000.0

Use arrow keys to change syringe size

ENTER ESCAPE

Now, use the arrow keys to increase or decrease the syringe size. When you have selected the correct size, press ENTER.

✱ Note: You must use the arrow keys to change syringe sizes. ✱

Chapter 3 Using the MICROLAB 500 System 3–15

7. Press ACCEPT to accept all syringe size values shown on the screen. The “Method type to create” menu appears:

Method type to create

Aliquot dispense method Serial dispense method Dilute method

Pipette method

Titrate method Custom method

SELECT menu item

SELECT ESCAPE

8. SELECT a method type. Each individual method consists of a series of screens and prompts that are described in detail. Refer to the following sections for information about creating specific methods.

9. When you are finished creating a method, the “Save method in method memory” screen appears. This screen shows the name of the new method.

Save method in method memory

Method: NEW1 will be stored in method memory.

CONFIRM to save, ESCAPE to cancel

ESCAPE CONFIRM

– Press CONFIRM to save the method, then press any key to continue. – Press ESCAPE to return to the previous screen.

3–16 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Creating an Aliquot Dispense Method

Use this function to create an aliquot dispense method. An aliquot dispense method consecutively dispenses equal amounts of liquids.

1. Follow steps 1–8 under “Creating a New Method.” Select the “Aliquot dispense method” function from the “Method type to create” menu. The “Aliquot dispense parameters” screen appears:

Aliquot dispense parameters

Left Right Aliquot volume (uL): 2500.0 250.0 Maximum aliquots: 1 1 Aliquots to dispense: 1 Syringe fill speed: 4 2 Syringe dispense speed: 4 2 Syringe fill mode: AUTO

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

2. Enter the volume(s) to be aliquoted. To enter a volume, use the arrow keys to highlight the Aliquot volume field, then press SELECT. The highlighting changes to underlining. Use the number keys to enter a new volume. When satisfied with your entry, press ENTER. The underline disappears and the highlighting reappears.

✱ Note: You cannot enter a value in the Maximum Aliquots field.

The system automatically calculates Maximum Aliquots: Maximum Aliquots = Total volume / Aliquot volume ✱

3. Enter the number of aliquots to be dispensed. Note that this number cannot be larger than the calculated number in the Maximum Aliquots field.

4. Enter the syringe fill speed.

5. Enter the syringe dispense speed.

Chapter 3 Using the MICROLAB 500 System 3–17

6. Enter the syringe fill mode; this mode can be either Auto or Manual. Use the arrow keys to change the current mode.

– Auto mode means that the instrument will perform the fill function

automatically when you run the method.

– Manual mode means you must press the Run/Stop key or press the probe

button to activate the fill function when you run the method.

7. When you are satisfied with your entries, press ACCEPT. The instrument displays the “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

Creating a Serial Dispense Method

Use this function to create a serial dispense method that serially dispenses various amounts of liquids.

1. Follow steps 1–8 under “Creating a New Method.” Select the “Serial dispense method” function from the “Method type to create” menu. The “Serial dispense volumes” screen appears:

Serial dispense volumes (uL)

Left Used 2500.0 Right Used 250.0 Available 0.0 Available 0.0

1. 2500.0 250.0

5. SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT DELETE

2. Enter the volumes to be dispensed from the syringes. Notice that default values to be dispensed are 100%, with the amount available 0%. As soon as you enter an amount to be dispensed, the system recalculates the volume available.

3–18 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

✱ Note: Notice that default value for the “Used” field is 100% of the

syringe size, and the default value for the “Available” field is 0% of the syringe size. As enter amounts to be dispensed, the system recalculates the volumes:

Used = Sum of all dispense amounts entered on all lines Available = (Syringe size - Used) ✱

– Enter up to 20 command lines; the screen will scroll as you add lines. If you

enter more than 20 lines, you return to line one.

3. Press DELETE to delete an entry. Use the arrow keys to move about from line to line. When you are finished entering amounts to be dispensed, press ACCEPT.

4. The system displays the “Serial dispense method parameters” screen:

Serial dispense method parameters

Dispenses: 1 Left Right Fill volume (uL): 2500.0 250.0

Syringe fill speed: 4 2 Syringe dispense speed: 4 2 Syringe fill mode: AUTO

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

5. Enter syringe fill speeds.

6. Enter syringe dispense speeds.

7. Select a syringe fill mode. Valid modes are AUTO or MANUAL. – Auto mode means that the instrument will perform the fill function

automatically when you run the method.

– Manual mode means you must press the Run/Stop key or press the probe

button to activate the fill function when you run the method.

8. When you are satisfied with your entries, press ACCEPT. The system displays the “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

Chapter 3 Using the MICROLAB 500 System 3–19

Creating a Dilution Method

Use this function to create a dilution method to perform the following tasks:

• Fill the diluent syringe (if using a two-syringe instrument, the left syringe) with a specified volume.

• With or without a time delay (of up to 60 seconds), aspirate an air gap volume and then a sample volume into the left syringe (if using a single-syringe instrument) or into the right syringe (if using a two-syringe instrument).

• Dispense the diluent and the sample volumes.

• Wash the probe tip up to one full cycle.

1. Follow steps 1–8 under “Creating a New Method.” Select “Dilute method” from the “Method type to create” screen. The “Dilute method” screen appears:

Dilute method

Ratio 1: 10.0 Dilution 1/ 11.0

Left Diluent volume (uL): 2500.0 Right Air gap volume (uL): 0.0 Right Sample volume (uL): 250.0 Final volume (uL): 2750.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

2. Enter two values in this screen. Choose from among the following pairs of numbers to enter:

• Ratio and Diluent volume

• Ratio and Sample volume

• Ratio and Final volume

• Diluent volume and Sample volume

• Diluent volume and Final volume

• Sample volume and Final volume

3–20 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

3. You may, but are not required to, enter an air gap volume. – If using a one syringe instrument, Final volume plus Air gap volume cannot

exceed 105% of the syringe size selected.

– If using a two syringe instrument, Diluent volume cannot exceed 105% of the

left syringe size selected. Sample volume plus Air gap volume cannot exceed 105% of the right syringe size selected.

4. Press ACCEPT and the controller calculates the other values

✱ Note: Ratio = 1 : (Diluent volume / Sample volume)

Dilution = 1 / ([Diluent volume + Sample volume] / Sample Volume), that is, Dilution = Ratio/(Ratio + 1)

Final volume = Diluent volume + Sample volume The resolution of the installed syringes may limit the system’s

ability to accommodate your exact dilutions or ratios. The system will try to calculate the closest possible combinations. If such combinations cannot be achieved using the installed syringes or the total volumes, the system will display an error message stating

the problem. ✱

6. The “Dilute method parameters” screen appears:

Dilute method parameters

Left Right

Syringe fill speed: 4 Syringe aspirate speed: 2 Syringe dispense speed: 4 2 Syringe fill mode: AUTO Air gap mode: N/A Air gap delay: N/A

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

7. Enter a fill speed for the left syringe.

8. Enter an aspirate speed for the right syringe.

9. Enter the dispense speeds for both syringes.

Chapter 3 Using the MICROLAB 500 System 3–21

10. Select a syringe fill mode. Valid modes are AUTO or MANUAL. – Auto mode means that the instrument will perform the fill function

automatically when you run the method.

– Manual mode means you must press the Run/Stop key or press the probe

button to activate the fill function when you run the method.

11. You may, but are not required to, enter an air gap volume. – If you enter an air gap volume greater than 0, specify a mode type. Valid

mode types are either MANUAL or AUTO.

- If you select MANUAL mode, you must pick up the air gap manually by using the Run/Stop key or the probe button.

- If you select AUTO mode, the instrument will pick up the air gap automatically. You may select a time delay period, from 0 to 60 seconds. The default is 0.3 seconds.

- If you do not select an air gap volume, “N/A” appears in the air gap mode and delay fields on the screen.

12. The “Probe wash parameters” screen appears:

Probe wash parameters

Wash volume (uL) 0.0

Left Syringe fill speed: 4 Left Syringe dispense speed: 4

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

Enter fill and dispense speeds. The syringe automatically fills with the wash volume. However, you must use the Run/Stop key or the probe switch to complete the wash cycle.

3–22 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

– If you are using a ML510B or a ML530B, you can wash the probe directly

from the left syringe.

– If you are using a ML540B, you cannot wash the right syringe or probe.

13. When you are satisfied with your entries, press ACCEPT. The system displays the “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

Creating a Pipette Method

Use this function to create a pipette method. A pipette method aspirates the same volume as it dispenses. The total actual volume dispensed, however, may include an air gap volume. A pipette method performs the following tasks:

• With or without a time delay (of up to 60 seconds), aspirate an air gap volume and then aspirate an amount of sample fluid into the left syringe (if using a single syringe instrument) or into the right syringe (if using a dual syringe instrument).

• Dispense both the sample fluid and the air gap volumes from the syringe.

• Wash the syringe.

1. Follow steps 1–8 under “Creating a New Method.” Select the “Pipette method” function from the “Method type to create” menu. The “Pipette parameters” screen appears:

Pipette parameters

Right Air gap volume 0.0 Right Dispense volume 250.0 Right Syringe aspirate speed: 2 Right Syringe dispense speed: 2 Air gap mode: N/A Air gap delay: N/A

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

Chapter 3 Using the MICROLAB 500 System 3–23

2. You may, but are not required to, enter a Right Air gap volume. – If you enter an air gap volume greater than 0, specify a mode type. Valid

mode types are either MANUAL or AUTO.

- If you select MANUAL mode, you must pick up the air gap manually by using the Run/Stop key or the probe button.

- If you select AUTO mode, the instrument will pick up the air gap automatically. You may select a time delay period, from 0 to 60 seconds. The default is 0.3 seconds.

- If you do not select an air gap volume, “N/A” appears in the air gap mode and delay fields on the screen.

3. Enter the Right Dispense volume.

4. Enter a Right Syringe aspirate speed.

5. Enter Right Syringe dispense speed.

6. The “Probe wash parameters” screen appears:

Probe wash parameters

Wash volume (uL) 0.0

Left Syringe fill speed: 4 Left Syringe dispense speed: 4

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

7. Enter fill and dispense speeds. The syringe automatically fills with the wash volume. However, you must use the Run/Stop key or the probe switch to complete the wash cycle.

– If you are using a ML510B or a ML530B, you can wash the probe directly

from the left syringe.

– If you are using a ML540B, you cannot wash the right syringe or probe.

3–24 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

8. When you are satisfied with your entries, press ACCEPT. The system displays the “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

Creating a Titrate Method

Use this function to create a titrate method. A titrate method performs the following functions:

• Fill the left and/or right syringes.

• Dispense an initial volume(s) from the syringe(s).

• Dispense step volume(s) from the syringe(s).

✱ Note: A titrate method is an interactive method that requires

user input each time it is run. For example, you determine whether or not to perform the next step of the method, based on your observations of the previous dispensation. Since all titration methods are interactive, you cannot copy a titration method to a

custom method or download a titration method to the drive unit. ✱

1. Follow steps 1–8 under “Creating a New Method.” Select the “Titrate method” function from the “Method type to create” menu. The “Titrate” screen appears:

Titrate

Left Right Fill volume (uL): 2500.0 250.0 Initial volume (uL): 0 .0 0.0 Step volume (uL): 2.5 0.25

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

Chapter 3 Using the MICROLAB 500 System 3–25

2. Enter a fill volume. The default volume is that of the installed syringe. – The fill volume must at least equal the initial volume plus one step. – The fill volume for at least one syringe must be greater than zero (0).

3. Enter an initial volume. The initial volume may be zero, less than the step volume, or greater than the step volume. Generally, however, initial volume is greater than the step volume.

4. Enter a step volume. This volume must be larger than zero (0) for at least one syringe.

5. The “Titrate method parameters” screen appears:

Titrate method parameters

Left Right

Syringe fill speed: 4 2 Initial dispense speed: 4 2 Step dispense speed: 8 8 Step auto-repeat delay: 0.3

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

6. Enter syringe fill speed(s).

7. Enter initial dispense speed(s).

8. Enter step dispense speed(s).

9. Enter a step auto-repeat delay; this is the time delay between two dispensations when you press the probe button or the Run/Stop key. Valid values are from 0–60 seconds.

10. When you are satisfied with your entries, press ACCEPT. The “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

3–26 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Creating a Custom Method

Use this function to create a custom method of your own design.

• You may enter up to 50 command lines; any command line may be executed either manually or automatically. If you execute commands automatically, you may delay the execution of each line by 0–60 seconds, in increments of 0.1 seconds.

• Valid syringe operation modes are listed in the following table:

Fill

Fills or picks up liquid from the valve input port (typically, this is from the reservoir via the diluent syringe).

Return Aspirate Dispense

✱ Note: On MICROLAB 530 instruments (D-type syringes, right side

only), Fill and Aspirate perform the same function. Also, Return and Dispense perform the same function. ✱

Dispenses or returns liquid to the valve input port. Aspirates or picks up liquid from the output port. Dispenses liquid to the valve output port.

• You can create a custom method to run a valve without moving the attached syringe if you set the appropriate volume to zero or if you do not enter a volume for the syringe.

1. Follow steps 1–8 under “Creating a New Method.” Select the “Custom method” function from the “Method type to create” menu. The “Custom method” screen appears. Use the arrow keys to move from field to field. You may enter values in any order.

Chapter 3 Using the MICROLAB 500 System 3–27

Custom method

Auto Dly Vol---LEFT-Spd Vol--RIGHT-Spd 1: 2: 3: 4: 5: 6:

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT DELETE

2. Auto field: Valid modes are Y or N, indicating that AUTO delay is either on or off. If the Auto field is blank, AUTO delay is off.

3. DLY (Delay) field. Enter the number of seconds of delay between method steps. If the Delay field is left blank, there is no delay between method steps.

4. Vol---LEFT field: – Use the arrow keys to set the mode of the left syringe. Valid modes are:

Fil (Fill), Asp (Aspirate), Dis (Dispense), and Ret (Return fluid to the reservoir).

– Enter a volume for the left syringe. If the Volume field is left blank, syringe

volume is assumed to be 0.

5. Spd (Speed) field: Enter a speed for the left syringe from 1 to 250 seconds. Speed must be entered in whole seconds. If the Speed field is left blank, the default speed for the syringe is used.

6. Vol--Right field: – Use the arrow keys to set the operation mode of the right syringe. Valid

modes are: Fil (Fill), Asp (Aspirate), Dis (Dispense), and Ret (Return fluid to the reservoir).

– Enter a volume for the right syringe. If the Volume field is left blank,

syringe volume is assumed to be 0.

3–28 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

7. Spd (Speed) field: Enter a speed for the right syringe from 1–250 seconds. The speed must be entered in whole seconds. If the Speed field is left blank, the default speed for the syringe is used.

8. Continue adding entries, creating up to 50 lines of commands.

9. When you are satisfied with your entries, press ACCEPT. The controller displays the “Save method in method memory” screen. Press CONFIRM to save the method, then press any key to continue.

Running an Existing Method

Use this function to run an existing method. At least one method must exist in the method storage area for you to use this function. If no method exists, displays a “Method Memory Empty” error message.

1. From the Main menu, SELECT the “Run an existing method” function. The following screen appears:

Run an existing method

NEW1 NEW2 NEW3

SELECT an existing method

SELECT ESCAPE

2. Use the arrow keys to highlight a method name, then press SELECT. (You cannot SELECT an unused method location.)

Chapter 3 Using the MICROLAB 500 System 3–29

3. A screen verifying whether or not the correct syringes are installed appears:

Method type: Methodname

This method requires the following syringe size(s) to be installed on the drive unit.

Left Syringe size (uL): 2500.0 Right Syringe size (uL): 250.0

CONFIRM to continue, ESCAPE to exit

ESCAPE CONFIRM

4. The “Drive unit initialization” screen appears. You must respond to this screen before running the method.

Drive unit initialization

Any fluid in the syringes on the drive unit will now be purged. Please ensure the probe is directed towards a waste area.

CONFIRM to continue, ESCAPE to exit

ESCAPE CONFIRM

5. The contents of the selected method appears on-screen. Follow the on-screen instructions to run the method.

3–30 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

Editing an Existing Method

Use this function to edit an existing method. At least one method must exist in the method storage area for you to use this function.

1. From the Main menu, SELECT the “Edit an existing method” function. If at least one method exists, the following screen appears:

Edit an existing method

NEW1 NEW2 NEW3

SELECT an existing method

SELECT ESCAPE

2. Use the arrow keys to highlight an existing method, then press SELECT. (You cannot highlight an unused method.)

3. The contents of the selected method appears on-screen. Use the arrow keys to highlight the field you wish edit, then press SELECT. The highlighting changes to underlining. Use the arrow keys or the numeric keypad to change the underlined value. When you are satisfied with your edit, press ENTER to accept the new value.

4. SELECT another field to edit or press ACCEPT to save all changes made to the method and return to the “Edit an existing method” screen. SELECT another method to edit or press ESCAPE to return to the Main menu.

✽ Note: Syringe size or configuration cannot be edited from this

screen.

✽

Chapter 3 Using the MICROLAB 500 System 3–31

Performing Manual Dilutions

Use the Manual Dilution function to perform manual dilutions without creating and saving a new dilution method, or without running an existing dilution method. Manual dilutions cannot be stored in memory.

1. From the Main menu, select the “Manual dilution” function. The “Instrument configuration” screen appears:

Instrument configuration

One syringe instrument

Two syringe instrument

SELECT menu item

SELECT ESCAPE

2. Use the arrow keys to highlight either a one- or a two-syringe instrument configuration, then press SELECT.

3. The default value(s) for the selected configuration appear on-screen.

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 2500.0 Right Syringe size (uL): 250.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

3–32 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

4. Press ACCEPT to retain the default sizes.

5. To change a syringe’s size, use the arrow keys to highlight the appropriate size, then press SELECT. The highlighting changes to underlining. Use the arrow keys to increase or decrease syringe size. Press ENTER to accept the new size. When you are satisfied with the syringe sizes, press ACCEPT.

6. The “Manual dilution” screen appears. The following sample screen shows a two-syringe instrument:

Manual dilution

Left Right Syringe speed: 4 2

• Fill volume (uL): 2500.0 Aspirate volume (uL): 0.0 250.0 Dispense volume (uL): 0.0 0.0

SELECT item to edit-RUN to start

SELECT ESCAPE MODE

7. Enter syringe speed(s). – Enter a fill volume for the left syringe. – Enter an aspiration volume for the left and right syringes. – You cannot enter a value for Dispense volume.

Dispense volume = Fill volume + Aspirate volumes.

8. Press CONFIRM.

9. Press the Run/Stop key to start the manual dilution method. The dot in the left margin moves to indicate the active mode. Press MODE for manual dilution editing options.

10. Press ESCAPE to exit the method and return to the main menu.

Chapter 3 Using the MICROLAB 500 System 3–33

Performing Manual Dispenses

Use the Manual Dispense function to perform manual dispensations without creating and saving a new dispense method, or without running an existing dispense method. Manual dispenses cannot be stored in memory.

1. From the Main menu, select the “Manual dispense” function. The “Instrument configuration” screen appears:

Instrument configuration

One syringe instrument

Two syringe instrument

SELECT menu item

SELECT ESCAPE

2. Use the arrow keys to highlight either a one- or a two-syringe instrument configuration, then press SELECT.

3. The default value(s) for the selected configuration appear:

Instrument configuration

One syringe instrument Two syringe instrument

Left Syringe size (uL): 2500.0 Right Syringe size (uL): 250.0

SELECT item to edit-ACCEPT if complete

SELECT ESCAPE ACCEPT

3–34 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

4. Press ACCEPT to retain the default sizes.

5. To change a syringe’s size, use the arrow keys to highlight the size, then press SELECT. The highlighting changes to underlining. Use the arrow keys to increase or decrease syringe size. Press ENTER to accept the new size; the previous screen appears. When you are satisfied with the syringe sizes, press ACCEPT.

6. The “Manual dispense” screen appears:

Manual dispense

Left Right Syringe speed: 4 2

• Fill volume (uL): 2500.0 250.00 Dispense volume (uL): 2500.0 250.00

SELECT item to edit-RUN to start

SELECT ESCAPE MODE

7. Enter syringe speed(s). – Enter a fill volume for the left and right syringes. – Enter a dispense volume for the left and right syringes.

8. Press CONFIRM.

9. Press the Run/Stop key to start the manual dispense method. The dot in the left margin moves to indicate the active mode. Press MODE for manual dispense editing options.

10. Press ESCAPE to exit the method and return to the Main menu.

Chapter 3 Using the MICROLAB 500 System 3–35

The Utilities Menu

The Utilities menu provides you with tools to manipulate methods and to diagnose and configure the instrument. Figure 3–5 shows the functions available from the Utilities menu.

Figure 3–5 The Utilities Menu

Utilities Menu

Confirm and

Utilities menu

Change

default

language

Select

language

Rename

existing method

Select

method

Rename

method

return to

Confirm and

return to

Utilities menu

Set

baud

rate

Select

rate

Delete existing method

Select

method

Copy method

Utilities menu

Turn

caps lock

on/off

Toggle

caps lock

to custom

method

Select

method

Name

new

method

Save and

return to

Save and

return to

Utilities menu

Confirm and

Utilities menu

Perform

display

test

Run test

Download method to

base unit

Select

method

return to

Diagnostics

configuration

Perform

keyboard

test

Run

test

and

Set

diagnostic

mode

Select

mode

3–36 MICROLAB 510B/511C, 530B/531C, and 540B/541C User’s Manual

The following screen shows the contents of the Utilities menu:

Utilities menu

Rename an existing method

Delete an existing method Copy a method to a custom method Download a method to the drive unit Diagnostic and configuration menu

SELECT menu item

SELECT ESCAPE

Use the arrow key to highlight the desired utility function, then press SELECT to accept the choice or press ESCAPE to return to the Main menu.

Renaming an Existing Method

Use this function to rename an existing method. At least one method must exist in the method storage area for you to use this function. If no method exists, receive “Method memory empty” error.

1. From the Utilities menu, SELECT the “Rename an existing method” function. The “Rename an existing method” screen appears:

Rename an existing method

NEW1 NEW2 NEW3

SELECT an existing method

SELECT ESCAPE

Chapter 3 Using the MICROLAB 500 System 3–37

Hamilton Microlab 500 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual