GE HEALTHCARE Typhoon FLA 7000 User Manual

GE Healthcare
Typhoon™ FLA 7000
User Manual
Table of Contents
51 Introduction ..........................................................................................................
51.1 Important user information .............................................................................................................
91.3 The Typhoon FLA 7000 laser scanner .........................................................................................
101.4 Typhoon FLA 7000 Control Software ...........................................................................................
152 Safety instructions ...............................................................................................
152.1 Introduction .............................................................................................................................................
152.2 General precautions ............................................................................................................................
162.3 Personal protection .............................................................................................................................
162.4 Laser safety .............................................................................................................................................
162.5 Radiation safety ....................................................................................................................................
172.6 Radiation hazard prevention ...........................................................................................................
182.7 Electric safety .........................................................................................................................................
192.8 Labels on Typhoon FLA 7000 ..........................................................................................................
232.9 Emergency procedures ......................................................................................................................
242.10 Recycling and disposal .......................................................................................................................
253 System configuration ..........................................................................................
253.1 Parts and functions ..............................................................................................................................
273.2 Instrument body ....................................................................................................................................
303.3 Accessories ..............................................................................................................................................
344 Installing and moving Typhoon FLA 7000 ........................................................
344.1 Site requirements ..................................................................................................................................
354.2 Transporting Typhoon FLA 7000 ...................................................................................................
354.3 Connections ............................................................................................................................................
375 Installing Typhoon FLA 7000 Control Software ..............................................
375.1 Installation sequence ..........................................................................................................................
375.2 Install Typhoon FLA 7000 Control Software (Windows XP) ...............................................
415.3 Install Typhoon FLA 7000 Control Software (Windows Vista) ..........................................
455.4 Install Typhoon FLA 7000 Control Software (Windows 7) ..................................................
536 Uninstalling and upgrading Typhoon FLA 7000 Control Software ..............
567 Registering, editing and deleting methods .....................................................
567.1 Registering a new method ...............................................................................................................
587.2 Editing a method ...................................................................................................................................
587.3 Deleting a method ................................................................................................................................
608 Filter module settings .........................................................................................
608.1 Registering a new filter name .........................................................................................................
608.2 Saving a filter combination ..............................................................................................................
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Table of Contents
618.3 Loading a filter combination ...........................................................................................................
628.4 Installing and replacing filters ........................................................................................................
669 Operation ..............................................................................................................
669.1 Operation overview .............................................................................................................................
669.2 Preparations before starting Typhoon FLA 7000 ...................................................................
679.3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software .......................
689.4 Selecting the reading mode .............................................................................................................
699.5 Setting the reading conditions .......................................................................................................
699.6 Placing the object to be scanned on the stage ......................................................................
739.7 Placing the stage in Typhoon FLA 7000 .....................................................................................
749.8 Scanning the sample ..........................................................................................................................
759.9 Adjusting the display parameters .................................................................................................
769.10 Saving the image data using a different file name ...............................................................
769.11 Viewing the image file in the analysis application ................................................................
769.12 Turning off Typhoon FLA 7000 .......................................................................................................
7710 Reading conditions, display parameters, and other settings ......................
7710.1 Reading conditions explained ........................................................................................................
8110.2 Display parameters explained ........................................................................................................
8210.3 Other settings .........................................................................................................................................
8511 About storage phosphor screens ......................................................................
8511.1 Introduction .............................................................................................................................................
8511.2 Compatible storage phosphor screens ......................................................................................
8511.3 Handling precautions .........................................................................................................................
8711.4 Preparing the storage phosphor screen ....................................................................................
8811.5 Exposing the storage phosphor screen .....................................................................................
9012 Maintenance .........................................................................................................
9213 Troubleshooting ...................................................................................................
97A Default sample detection methods ..................................................................
99B Specifications ........................................................................................................
4 Typhoon FLA 7000 User Manual 28-9607-62 AC
Table of Contents
1 Introduction
In this chapter
This chapter contains important user information, and a general description of Typhoon FLA 7000 and its intended use.
1.1 Important user information
Read this before using the Typhoon FLA 7000
All users must read this entire manual to fully understand the safe use of Typhoon FLA
7000.
Intended use
Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging, ECL Plus Westerns, visible fluorescence and gel documentation.
Typhoon FLA 7000 is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the safe use of Typhoon FLA 7000. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.
Typhoon FLA 7000 User Manual 28-9607-62 AC 5
1 Introduction
Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note:
A Note is used to indicate information that is important for trouble-free and optimal use of the product.
Tip:
A tip contains useful information that can improve or optimize your procedures.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (e.g., Power switch).
1.2 Regulatory information
This section lists the directives and standards that are fulfilled by the Typhoon FLA 7000 system.
6 Typhoon FLA 7000 User Manual 28-9607-62 AC
1 Introduction
1.1 Important user information
Manufacturing information
ContentRequirements
GE Healthcare Bio-Sciences AB, Björk­gatan 30, SE 751 84 Uppsala
Name and address of manufacturer
Sweden
INTERTEK SEMKO AB, NB 0413Name and ID of notified body
Uppsala, Sweden, Sept. 2009Place and date of declaration
See EC Declaration of ConformityIdentity of person authorized to sign
Declaration of Conformity
International standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
Safety requirements for electrical equipment for measurement, control, and laboratory use
EN 61010-1, IEC 61010-1, UL 61010-1, IEC 61010-2-81, CAN/CSA-C22.2 No. 61010­1
Harmonized with 2004/108/EC
EMC emissions and immunity requirements for electrical equipment for measurement, control and laboratory use
EN 61326-1
VCCI Class A
FCC Part 15 B Class A
ICES-003 Class A
Harmonized with 2006/42/EC
Safety of machinery - Basic con­cepts, general principles for de­sign
EN-ISO 12100-1, 12100-2
Harmonized with 2006/42/EC
Safety of machinery - Principles of risk assessment
EN-ISO 14121-1, 14121-2
Harmonized with 2006/95/EC
Safety of laser productsEN 60825-1, IEC 60825-1
Safety of laser productsUSA 21 CFR, Chapter I, Sub­chapter J, Part 1040.10 Laser Products
Typhoon FLA 7000 User Manual 28-9607-62 AC 7
1 Introduction
1.2 Regulatory information
CE Conformity
This product complies with the European directives listed in the table, by fulfilling the corresponding harmonized standards. A copy of the Declaration of Conformity is available on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Low Voltage Directive (LVD)2006/95/EC
ElectroMagnetic Compatibility (EMC) Directive2004/108/EC
CE Marking
The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is:
used as a stand-alone unit, or
connected to other CE marked GE Healthcare instruments, or
connected to other products recommended or described in the user documentation, and
used in the same state as it was delivered from GE Healthcare, except for alterations described in the user documentation.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.
This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numerique de la class A est conforme a la norme NMB-003 du Canada.
8 Typhoon FLA 7000 User Manual 28-9607-62 AC
1 Introduction
1.2 Regulatory information
Note:
This is a class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.
Laser standards
This instrument is a Class 1 (IEC60825-1:2007/EN60825-1:2007) laser product.
The following lasers can be installed in Typhoon FLA 7000:
Maximum powerWavelengthLaser and class
20 mW (CW)473 nmLD laser, class 3B
10 mW (CW)532 nmSHG laser, class 3B
45 mW (CW)635 nmLD laser, class 3B
80 mW (CW)650 nmLD laser, class 3B
Note:
The beam divergence of all laser modules is collimated.
Regulatory compliance of connected equipment
Any equipment connected to the Typhoon FLA 7000 should meet the safety requirements of EN 61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment must be CE marked.
1.3 The Typhoon FLA 7000 laser scanner
The compact Typhoon FLA 7000 is an image analyzer that offers high performance, multifunctionality, and high-speed reading. Benefits include:
Typhoon FLA 7000 User Manual 28-9607-62 AC 9
1 Introduction
1.2 Regulatory information
Detection of high-sensitivity autoradiographs using storage phosphor screens.
Four lasers and multiple filters enable the use of a variety of fluorescent dyes and detection methods.
Data is aquired through high resolution, sensitivity and wide range linearity.
Compatibility with various types of samples, for example fluorescent labels, fluorescent dyes, CBB stain, silver stain, X-ray film, and storage phosphor screens.
1.4 Typhoon FLA 7000 Control Software
System requirements
Minimum requirementParameter
Microsoft™ Windows™ XP Professional SP3 (32-bit)Operating system
or
Microsoft Windows Vista Business SP2 (32-bit)
or
Microsoft Windows 7 Professional (32-bit)
1 GBInternal memory
Intel Core 2 Duo processorProcessor
80 GBHard drive
1280 × 1024 pixelsMonitor resolution
One USB 2.0 portOther requirements
DVD-ROM drive
10 Typhoon FLA 7000 User Manual 28-9607-62 AC
1 Introduction
1.3 The Typhoon FLA 7000 laser scanner
Overview of the main window
The Typhoon FLA 7000 Control Software is used to control, use and supervise the Typhoon FLA 7000.
1
2
3
4
5
6 7 8
FunctionPart
Phosphorimaging button: click to read a storage phosphor screen1
Fluorescence button: click to read a fluorescent sample2
Digitization button: click to perform digitization3
Method button: click to register or erase a combination of laser and filter.4
Filter Module button: click to change or register a filter.5
Preferences button: click to set the image file format, scan mode, correction
mode and to enable or disable the ND filter.
6
Filter: displays the loaded filters.7
Laser: displays the status of the loaded laser units8
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1 Introduction
1.4 Typhoon FLA 7000 Control Software
Overview of the reader settings window
1
2
3
4
7
6
12
13
10
11
8
5
9
14
15
DescriptionPart
Image folder: specify where to save the file after the reading.1
File Name: enter the name of a file to save image data.2
Comment: enter an optional comment. The comment is embedded
in the file where the image is saved, and can be viewed with the analyzing software.
3
Method: set the method to use in the scan. Up to 4 scans can be performed in a row, all with individual methods.
4
PMT: set the voltage of the photo-multiplier tube. The higher the value, the higher the sensitivity.
5
Click to increase the number of
scans
Click to decrease the number of
scans
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1 Introduction
1.4 Typhoon FLA 7000 Control Software
DescriptionPart
Set the scanning area.6
1
Select a stage in the drop-down menu.
2
Drag the red square to the desired position of the scanning area.
3
Drag the sides of the red square as needed to adjust the size of the scanning area.
Pixel Size: set the pixel size.7
Choose a small pixel size for high quality images. Note that a small pixel size setting increases the reading time and the size of the image file.
Latitude: Specify the dynamic range.8
L5 provides a larger detectable range than L4.
L4 provides a finer density gradation, if the signals of the sample areas are in the correct range.
Mode: Specify the mode for the reading area borders.9
Select Grid to limit the borders to the grid lines.
Select Free for an arbitrary area.
Select All to use the entire Fluor stage as the reading area.
Save Condition...: click this button to save the current reading conditions in a file, if desired. For details, refer to the User Manual.
10
Load Condition...: click this button to load previously saved reading conditions, if desired. For details, refer to the User Manual.
11
File Size: the estimated size of the result data file is presented.12
Reading Time: the estimated time required for the scan is present-
ed.
13
Top: return to the main window.14
Start Scan: start the scan. The sample must be loaded before
starting a scan.
15
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1 Introduction
1.4 Typhoon FLA 7000 Control Software
System requirements
Minimum requirementParameter
Microsoft™ Windows™ XP Professional SP3 (32-bit)Operating system
or
Microsoft Windows Vista Business SP2 (32-bit)
or
Microsoft Windows 7 Professional (32-bit)
1 GBInternal memory
Intel Core 2 Duo processorProcessor
80 GBHard drive
1280 × 1024 pixelsMonitor resolution
One USB 2.0 portOther requirements
DVD-ROM drive
14 Typhoon FLA 7000 User Manual 28-9607-62 AC
1 Introduction
1.4 Typhoon FLA 7000 Control Software
2 Safety instructions
2.1 Introduction
The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that may be hazardous. Before installing, operating or maintaining the equipment , you must be aware of the hazards described in the user documentation. Follow the instructions provided to avoid personal injury or damage to the equipment.
2.2 General precautions
WARNING
Do not use the equipment if smoke, strange noises or strange odors can be perceived, or if the equipment becomes unusually hot. This may result in fire or electric shock.
Stop using immediately, turn off the power switch and unplug the equipment from the power outlet. Contact your local GE Healthcare representative to request repair.
WARNING
Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE Healthcare representative for replacements.
WARNING
Do not place the equipment on unstable tables or on inclined surfaces, as the equipment could be dropped or fall, resulting in injury.
Typhoon FLA 7000 User Manual 28-9607-62 AC 15
2 Safety instructions
2.3 Personal protection
CAUTION
Always wear gloves, protective glasses and a lab coat or similar when handling samples.
CAUTION
Always wear cotton gloves when handling storage phosphor screens.
2.4 Laser safety
WARNING
Never detach the inner cover screwed to this instrument. If it is detached, laser beam may leak with a risk of loss of vision.
CAUTION
Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to laser radiation.
2.5 Radiation safety
Radiation safety
This instrument is not equipped with any radioisotope (RI) or radiation generating unit, and is therefore not regulated by radiation hazard prevention laws. However, the instrument is capable of reading Storage phosphor screens which may be polluted by radioisotopes (RI).
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument immediately and follow the instructions of your radiation administrator.
16 Typhoon FLA 7000 User Manual 28-9607-62 AC
2 Safety instructions
2.3 Personal protection
2.6 Radiation hazard prevention
Controlled area
Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56) defines the controlled area as "a place where the dose equivalent related to external radiation exceeds the dose equivalent determined by the Director General of the Science and Technology Agency (hereinafter referred to as the Director General), the concentration of radioisotope in the air exceeds the concentration determined by the Director General, or the radioisotope density on the surface polluted by radioisotope exceeds the density determined by the Director General."
Limit of superficial pollution
Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that determines the quantity, etc. of radiating isotope specifies that the density of radioisotope on the surface polluted by radioisotope must be one tenth of the density defined in Article
8.
Article 8 and Table 3 of this Notice define the limits as shown below:
1
Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm
2
2
Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm
2
Installation site of instrument
This instrument is capable of reading not only Storage phosphor screens but also fluorescent pigment label samples (non-RI method). Therefore, it is recommended that the user should install it outside the controlled area and use RI-indicated samples without contacting them with Storage phosphor screens directly.
However, as described above, the Storage phosphor screen surface may be polluted by radioisotope (RI), depending on the sample condition, since the instrument sticks the sample to the3H-compatible Storage phosphor screen surface and exposes it in an auto-radiography experiment of the3H label sample.
When a sample is in direct contact with a Storage phosphor screen, it is generally known that the sample for making an auto-radiogram contains a relatively small quantity of radioisotope. However, the degree of superficial pollution of the Storage phosphor screen is greatly influenced by the dryness of the sample and dose of radioisotope in an experiment and may exceed the limits mentioned in Limit of superficial pollution , on page 17.
When the instrument reads a Storage phosphor screen with a polluted non-exposure area, it may be polluted. The degree of such superficial pollution greatly differs with users' operation conditions. Superficial pollution may exceed the limit mentioned above.
Note:
As mentioned above, install this instrument in the RI controlled area if the user uses RI-indicated samples that will be in direct contact with Storage phosphor screens.
Typhoon FLA 7000 User Manual 28-9607-62 AC 17
2 Safety instructions
2.6 Radiation hazard prevention
Removal from the controlled area
If it is necessary to move the instrument and its laboratory, which were installed and have been used in the controlled area, from the controlled area, make sure that the degree of the superficial pollution is below the limits mentioned in Limit of superficial pollution above.
2.7 Electric safety
WARNING
Do not use the equipment with a power supply other than that recommended. Fire and electric shock could result.
WARNING
Do not use the equipment within or near a sink, or in humid or dusty environments. Fire and electric shock could result.
WARNING
Connect the power supply directly to a grounded wall power outlet. The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock.
CAUTION
Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge. Malfunction could result.
18 Typhoon FLA 7000 User Manual 28-9607-62 AC
2 Safety instructions
2.6 Radiation hazard prevention
2.8 Labels on Typhoon FLA 7000
Typhoon FLA 7000 serial number
The Typhoon FLA 7000 serial number is located on a label on the back of the instrument.
123456
Symbols used in safety labels
MeaningLabel
Warning! Read the user documentation before using the system. Do not
open any covers or replace parts unless specifically stated in the user documentation.
The system complies with the requirements for electromagnetic compliance (EMC) in Australia and New Zealand.
The system complies with applicable European directives.
Typhoon FLA 7000 User Manual 28-9607-62 AC 19
2 Safety instructions
2.8 Labels on Typhoon FLA 7000
Labels concerning use of hazardous substances
MeaningLabel
This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufac­turer for information concerning the decommissioning of equipment.
Labels concerning laser light
MeaningLabel
CAUTION! Avoid exposure to the laser
beam when the lid is open.
CAUTION! Class 3B Laser product when open and interlock defeated. Avoid expo­sure to the beam.
Do not attempt to defeat the safety inter­locks under the sample lid or behind the filter door, or otherwise try to gain access to the interior of the instrument through any other opening. Exposure to laser light can cause injury. Viewing the laser light directly can cause blindness.
20 Typhoon FLA 7000 User Manual 28-9607-62 AC
2 Safety instructions
2.8 Labels on Typhoon FLA 7000
Labels at the front of Typhoon FLA 7000
Typhoon FLA 7000 User Manual 28-9607-62 AC 21
2 Safety instructions
2.8 Labels on Typhoon FLA 7000
Labels at rear of Typhoon FLA 7000
123456
22 Typhoon FLA 7000 User Manual 28-9607-62 AC
2 Safety instructions
2.8 Labels on Typhoon FLA 7000
Labels on left side of Typhoon FLA 7000
I : Power ON O : Power OFF
USB
2.9 Emergency procedures
This section describes how to do an emergency shutdown of the Typhoon FLA 7000 instrument. The section also describes the results of a power failure.
Emergency shutdown
ActionStep
Click the Stop button in the Typhoon FLA 7000 Control Software.1
Turn off the Typhoon FLA 7000 by pressing the power switch on the left side of the instrument body.
2
Disconnect the power cord from the power outlet .3
Typhoon FLA 7000 User Manual 28-9607-62 AC 23
2 Safety instructions
2.8 Labels on Typhoon FLA 7000
Power failure
The results of a power failure depends on the unit or units affected.
ResultsUnit affected by power failure
The reading is interrupted immediately. The instrument is in an undefined state.
The data collected up to the time of the power failure is available in the file created when starting the scan.
Typhoon FLA 7000 instrument
The computer shuts down immediately.
The run continues, but no data is saved.
Computer running the Typhoon FLA 7000 Control Software
2.10 Recycling and disposal
General instructions for disposal
When taking the Typhoon FLA 7000 out of service, the different materials must be separated and recycled according to national and local environmental regulations.
Specific instructions for disposal
Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual.
If the pollution level exceeds the limit, dispose of the instrument body as radioactive waste. Otherwise, dispose of the materials according to applicable laws and regulations for disposal of industrial waste.
24 Typhoon FLA 7000 User Manual 28-9607-62 AC
2 Safety instructions
2.9 Emergency procedures
3 System configuration
3.1 Parts and functions
The parts and their functions are explained separately in this chapter.
1
2
3
4
5
6
7
8
9
A
B
C
D
E F G H
I J
K
L
M
N O
P
A
B C
D
E
F G
H
I
J K L
M N
O
P
A B C D
E
F G H I
J
K
L
M N O
P
A B C D E
F
G H
I J K
L
M
N O P
1
2
3
4
5
6
7
8
9
FunctionPart
Power switch, located between the AC connector and the USB connector1
Indicator lamps2
Knob3
Lid4
Typhoon FLA 7000 User Manual 28-9607-62 AC 25
3 System configuration
FunctionPart
Setting block5
Filter change door6
Filter module slot7
Filter module8
Filter9
Phosphor stage10
Suction rod11
Protection cover for storage phosphor screen12
Fluor stage13
Digitizing fluorescent plate14
Membrane weight (included in Fluor stage)15
26 Typhoon FLA 7000 User Manual 28-9607-62 AC
3 System configuration
3.1 Parts and functions
3.2 Instrument body
Power switch
The power switch is located on the left side of the Typhoon FLA 7000.
Inlet
Cooling fans
The instrument has two cooling fans on the back side.
CAUTION
Do not block the cooling fans with a wall or objects. If they are blocked, the instrument may become damaged.
Typhoon FLA 7000 User Manual 28-9607-62 AC 27
3 System configuration
3.2 Instrument body
Air intake fan
Exhaust fan
Indicator lamps
Three indicator lamps indicate the instrument status.
28 Typhoon FLA 7000 User Manual 28-9607-62 AC
3 System configuration
3.2 Instrument body
Stage setting block
Stage door
Knob
Stage setting block
Filter module
The filter module is located behind the filter door.
Filter module slot
Filter module
Filter change door
Typhoon FLA 7000 User Manual 28-9607-62 AC 29
3 System configuration
3.2 Instrument body
USB port
The USB port is located on the left side of Typhoon FLA 7000.
USB label
3.3 Accessories
Digitization plate (included)
The digitization plate is a fluorescent plate that emits light when exposed to a laser beam. This plate is used when the digitization mode is used.
30 Typhoon FLA 7000 User Manual 28-9607-62 AC
3 System configuration
3.2 Instrument body
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