FUJIFILM LAS-3000 Operation Manual
LUMINESCENT IMAGE ANALYZER
LAS-3000
OPERATION GUIDE
2nd Edition 2004. Jun.
Introduction
Thank you for purchasing the FUJIFILM Luminescent Image Ana-
lyzer LAS-3000.
This guide is used for explanation of the equipment. Therefore,
this guide simply describes each function and usage. Please see
the Operation Manual for more detailed informations.
Index
1 Features of the Luminescent Image Analyzer 3
2 System Configuration and Parts Identification 3
3 Preparation for Exposure 6
1 Activation 6
2 Setting of Samples 6
4 Exposure of Samples (Lite mode) 7
1 Setting of Mode 7
2 Method and Tray position Setting 7
3 Adjustment of Focus 8
4 Exposure Type and Exposure Time Setting 8
5 Setting of Sensitivity 8
6 Exposure 9
7 Saving the Exposed Image 9
8 Printing the Exposed Image 10
9 Termination 10
5 Exposure of Samples (Pro mode) 11
6 Cautions on Use 12
1 Sample Tray 12
Appendix 12
■ Exposure Type 12
■ Operation required to be carried out for exposure with
green and red LED incident light sources (Optional) 13
■ Detection Sensitivity and Number of Pixels 14
■ Detection Reagents and Settings of LAS-3000 14
2
∗ In LAS-3000, the excitation light (incident/transmitted light), filter, lens, and the components of the
analytic part vary depending on the system you are using. Please confirm each system. The basic
system of “LAS-3000” is described in this Operation Guide.
Features of the Luminescent Image Analyzer
1
In LAS-3000, chemiluminescent and fluorescent samples can be detected in high sensitivity, Dark frame
correction and Flat frame correction are equipped for the quantitative analysis of the images. A digitized
image of stained gel, membrane or film can be obtained by white light illumination.
● A super CCD camera of 3,200,000 pixels is employed. A high-resolution image of 6,300,000 pixels is
obtained by the special image processing procedure.
● A newly designed lens of F0.85 with remote focus and remote iris is mounted.
Chemiluminescence can be detected in high sensitivity through use of a four-step pixel binning function.
● Fluorescence can be detected in high sensitivity using a UV transmitted illuminator and incident blue LED.
● Wider range of fluorescent reagents can be detected by the use of green and red incident LEDs in addition
to the blue one (Optional).
● The operability is remarkably improved by the newly designed Image Reader software.
System Configuration and Parts Identification
2
< System configuration of LAS-3000: An Example > < Internal view of intelligent dark box (IDX) >
LAS-3000 OPERATION GUIDE
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4
7
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Name Description
Camera head CCD cooling function and image data output USB port
1
Intelligent dark box (IDX) Dark box
2
Lens unit F0.85/43mm
3
Incident light source
4
Transmitted light source 312nm UV light source or
5
8
Blue LED (460nm) incident light sources
White LED incident light sources
Green LED (520nm) incident light sources (Optional)
Red LED (630nm) incident light sources (Optional)
White transmitted light source
3
6
5
3
Filter Y515Di (filter for blue LED)
6
605DF40 (EtBr detecting filter)
510DF10 (GFP detecting filter)
575DF20 (filter for green LED) (Optional)
R670 (filter for red LED) (Optional)
Sample tray
7
EPI tray
(for chemiluminescence and incident light source)
DIA tray (for transmitted light source)
NP tray (for titer plate)
DIA-UV tray (for UV transmitted light source)
Computer Exposure operation and analysis work
8
● Pictrography High quality digital color printer
4
Image Reader Software
The Image reader software consists of Lite and Pro modes. In the Lite mode, luminescence can be easily
detected because a detection method has been set. In the Pro mode, advanced combination can be set.
< Screen in Lite mode >
LAS-3000 OPERATION GUIDE
Exposure Type
Sets the exposure method.
Exposure Time
Sets the exposure time.
Sensitivity
Sets the sensitivity.
Image acquire & Digitize
A digitized image is also exposed
consecutively with a Chemiluminescence image (only when the
method is Chemiluminescence).
The setting state such as
the temperature condition
of CCD is displayed.
The tray positon and light
conditions in the intelligent
dark box (IDX) is displayed.
Menu
Pro mode selector button
Press this button to switch to the Pro mode.
Method/Tray position
Sets the detection method
and tray position.
Focusing
Adjusts the focusing.
Start
Starts the
exposure.
< Screen in Pro mode >
Exposure Type
Sets the exposure method.
Exposure Time
Sets the exposure time.
Sensitivity/Resolution
Sets the sensitivity and resolution.
Invert Pixels
Exposes the image whose concentration value is inverted.
The setting state such as
the temperature condition
of CCD is displayed.
The tray positon and light
conditions in the intelligent
dark box (IDX) is displayed.
Menu
Lite mode selector button
Press this button to switch to the Lite mode.
Darkbox option setting
Sets the light source, filter, and
tray position.
Focusing
Adjusts the focusing.
Start
Starts the
exposure.
∗Please use the Pro mode, in case of using green LED or red LED incident light source. Please use the Lite
mode for imaging of chemiluminescence, digitizing, EtBr detection by UV light source, fluorescence detection by blue LED, using the preset conditions.
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