FUJIFILM LAS-1000plus Operation Manual

LUMINESCENT IMAGE ANALYZER

LAS-1000plus

Operation Manual

2nd Edition

2001. Oct. Version 2.0

Read this first

Thank you for purchasing the FUJIFILM Luminescent Image Analyzer LAS1000plus. The FUJIFILM Luminescent Image Analyzer LAS-1000plus uses a 1,300,000-pixel cooling CCD camera developed exclusively by Fuji Film and is a one-unit image analysis system that can be used for fluorescent and enzyme amplification fluorescent methods, and mainly for the chemical fluorescence method.

The "FUJIFILM Luminescent Image Analyzer LAS-1000plus Operation Manual" describes the operation methods and usage precautions for LAS1000plus so that you will be able to utilize the functions to the utmost and bring out its full performance. Please read this manual thoroughly before operating the LAS-1000plus. We suggest that you keep this manual, as you may need to refer to it.

* Image Reading Software "Image Reader Lite" and "Image Reader Pro"

described herein are available in both the Macintosh version and in the Windows version. In this manual, software operations are explained based on the Windowsversion displays, but operations using the Macintosh-version software are basically the same as those for the Windows-version software.

NOTES 1 Reproduction of parts or all of the contents of this manual without permis-

sion is prohibited.

2 The contents of this manual are subject to change without notice. 3 This manual has been prepared with utmost care. However, if you have

any questions or find errors, ommissions, etc., please contact us.

4 We will not be liable for any effects incurred from the use of this device.

This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations. Cet appareil numérique de la classe: A respecte toutes les exigences du Règlement sur le matériel brouilleur du Canada.

Apple logo is a registered trademark of Apple Computer, Inc. MacintoshTM is a registered trademark of Apple Computer, Inc. Windows®, Windows® 95 and Windows NT® are registered trademarks of Microsoft Co., Ltd. Other company names and product names mentioned in this document are the trademarks or registered trademarks of those companies.

2001. Oct. Version 2.0

This equipment cannot be taken to or used in a country or area where power supply specifications differ from those in the country or area where it was originally installed.

United States of America

This equipment generates, uses, and can radiate radio frequency energy and if not installed and used in accordance with the instructions manual, may cause interference to radio communications. It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of part 15 of FCC Rules, which are designed to provide reasonable protection against such interference when operated in a commercial environment. Operation of this equipment in a residential area is likely to cause interference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference.

Canada

This digital apparatus does not exceed the Class A limits for radio noise emission from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications.

“Le présent appareil numérique n’émet pas de brults radioélectriques cépassant les limites applicable aux appareils numériques de la class A prescrites dans le Réglement sur le broulilage radioélectrique édicté par le ministére des Communications du Canada.”

2001. Oct. Version 2.0

Contents

Part 1 Safety-Related Cautions ............................................................................................ 1

1. Cautions Prior to Use................................................................................................................................. 2

1.1 Icon Indicators ................................................................................................................................... 2

1.2 Examples of Icon Indicators............................................................................................................... 2

2. Usage Cautions ......................................................................................................................................... 8

2.1 Place of Installation............................................................................................................................ 8

2.2 Power Supply..................................................................................................................................... 9

2.3 Operational and Maintenance Cautions ............................................................................................ 10

n Operational Cautions ...................................................................................................................... 10

3. LED Light Source....................................................................................................................................... 11

LED Used .......................................................................................................................................... 11

Part 2 LAS-1000plus System Configuration ......................................................................... 13

1. LAS-1000plus System Hardware Configuration ........................................................................................ 14

2. Specifications............................................................................................................................................. 15

2.1 Performance Characteristics ............................................................................................................. 15

2.2 External Dimensions and Weight....................................................................................................... 15

2.3 Power Requirements ......................................................................................................................... 16

2.4 Physical Environment ........................................................................................................................ 16

Part 3 Software Installation ................................................................................................... 17

1. Introduction ................................................................................................................................................ 18

2. Software Installation and Uninstallation ..................................................................................................... 18

2.1 Software Installation (for Windows) ................................................................................................... 18

2.2 Software Uninstallation ...................................................................................................................... 24

2.3 Software Installation (for Macintosh) ................................................................................................. 26

3. MATROX Meteor II .................................................................................................................................... 27

3.1 Video Board Driver Installation .......................................................................................................... 27

Part 4 Operations I (Image Reader LAS-1000 Lite).............................................................. 33

1. Startup ....................................................................................................................................................... 34

2. Window Configuration................................................................................................................................ 36

3. Exposing Chemiluminescence Samples.................................................................................................... 41

4. Exposing Fluorescent Samples and Enzyme Amplification Fluorescent Samples (Fluorescence) ........... 53

5. Exposing Stained Gel or Film Samples ..................................................................................................... 65

6. Exposing Fluorescent Samples Using Optional Filter................................................................................ 77

7. Quitting....................................................................................................................................................... 78

Contents

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Part 5 Operations II (Image Reader LAS-1000 Pro) ............................................................. 79

1. Startup and Quitting ................................................................................................................................... 80

1.1 Startup ............................................................................................................................................... 80

1.2 Quitting .............................................................................................................................................. 80

2. 4 Modes ..................................................................................................................................................... 81

3. Mode Usage Methods................................................................................................................................ 83

3.1 Focusing ............................................................................................................................................ 83

3.2 Precision ............................................................................................................................................ 87

3.2.1 Setting Method ....................................................................................................................... 87

3.2.2 Exposure Startup.................................................................................................................... 94

3.2.3 Printing Images....................................................................................................................... 99

3.2.4 Saving Images........................................................................................................................ 99

3.3 Increment........................................................................................................................................... 100

3.3.1 Setting Method ....................................................................................................................... 100

3.3.2 Exposure Startup.................................................................................................................... 104

3.3.3 Saving Images........................................................................................................................ 106

3.3.4 Printing Images....................................................................................................................... 106

3.4 Repetition........................................................................................................................................... 107

3.4.1 Setting Method ....................................................................................................................... 107

3.4.2 Exposure Startup.................................................................................................................... 111

3.4.3 Saving Images........................................................................................................................ 113

3.4.4 Printing Images....................................................................................................................... 113

Part 6 Flat-Frame Maker Operation ...................................................................................... 115

1. Introduction ................................................................................................................................................ 116

2. Operation Procedures................................................................................................................................ 116

Part 7 Troubleshooting.......................................................................................................... 119

1. Error Codes Displayed on the Analyzing Unit and Countermeasures ....................................................... 120

2. Troubles and Countermeasures ................................................................................................................ 121

2.1 Camera Head Trouble Cause Analysis ............................................................................................. 121

2.2 Camera Controller and Reading Software Trouble Cause Analysis.................................................. 122

2.3 Dark Box Trouble Cause Analysis ..................................................................................................... 124

2.4 Image Trouble Cause Analysis.......................................................................................................... 125

3. Error Display Contents and Countermeasures .......................................................................................... 126

Part 8 Regular Maintenance ................................................................................................. 129

Intelligent dark box and camera controller maintenance ........................................................................... 130

Sample tray maintenance .......................................................................................................................... 130

Camera head, lens and optical filter maintenance..................................................................................... 130

Part 9 After-Sale Service ...................................................................................................... 131

1. Warranty .................................................................................................................................................... 132

2. Repairs....................................................................................................................................................... 132

TROUBLE FAX SHEET

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Part

Safety-Related Cautions

1. Cautions Prior to Use

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1. Cautions Prior to Use

1.1 Icon Indicators

* Make sure to have read the Operation Manual thoroughly before using

the equipment and to use the equipment properly. * After reading the Manual, store it where it can be referred to at any time. * Please note that the product specifications and operation manual are

subject to changes without notice.

The indicators in this Operation Manual and on the equipment are to ensure that you use the equipment safely and properly. Various icon indicators have been used to prevent injury to all users and property damage from occurring. These indicators and their meanings are explained below. Continue reading this manual after thoroughly understanding their content.

Indicates the possibility that a person could die or sustain serious injury if this indicator is ignored and the equipment is handled incorrectly.

Indicates that a person could sustain injury or there could be physical damage if this indicator is ignored and the equipment is handled incorrectly.

1.2 Examples of Icon Indicators

The indicator informs the user that the contents merit warning or caution.

The specific content of the prohibition is shown within the indicator (which on the left cautions against electric shock).

The indicator informs the user of prohibited actions.

The specific content of the prohibition is shown within or nearby the indicator (which on the left prohibits disassembly).

The indicator informs the user of actions that must be performed.

The specific content of the action is shown within the indicator (which on the left instructs the user to unplug a power supply plug from an electrical outlet). Make sure to do exactly as instructed.

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In the unlikely event that smoke can be perceived, the outside of the equipment becomes unusually hot, strange odors or noises can be perceived, etc., and the equipment is used under such abnormal conditions, fire and electric shock could result. Immediately suspend usage, turn OFF equipment power, and then unplug the power supply plug from the electrical outlet. Confirm that smoke no longer can be perceived and then contact your dealer to request repairs.

* In the unlikely event that water gets into the camera head and/or controller, first

turn OFF the equipment's power supply switch, unplug the power supply plug from the electrical outlet, and then contact your dealer. Using the equipment in this condition could result in fire and electric shock.

1. Cautions Prior to Use

Unplug the power supply plug from the electrical outlet.

* In the unlikely event that a foreign object gets into the dark box, camera head and/

or controller, first turn OFF the equipment's power supply switch, unplug the power supply plug from the electrical outlet, and then contact your dealer. Using the equipment in this condition could result in fire and electric shock.

* In the unlikely event that the equipment is dropped and the cabinet is damaged,

* Do not remove covers of the dark box, camera head and/or controller, because

inside are parts of high temperature and voltage that could result in burns or electric shock.Contact your dealer to request internal inspections, servicing, and repairs.

* Do not place on the dark box, camera head and/or controller vases, flowerpots,

cups, cosmetics, medical supplies, containers that have water or the like in them, or small metal objects. If such items are spilled or fall into the equipment, fire and electric shock could result.

* Do not use the equipment at other than the indicated power supply voltage or fire

and electric shock could result.

Disassembly prohibited

* Make sure to directly connect the power supply plug to a wall outlet (tripolar plug

socket) that has a ground terminal. Extension using table taps or putting many loads on one electrical outlet could result in fire and electric shock.

* Make sure that no water gets into the camera head and/or controller and that they

do not get wet or fire and electric shock could result.

Make sure to connect a ground wire.

1. Cautions Prior to Use

* Do not place or drop such foreign objects as metal or flammable objects into the

ventilation holes of this equipment or fire and electric shock could result.

* Make sure not to place heavy objects on the power supply cord and connection

cords, and do not let them get pinned under the equipment. Cords could be damaged by this, and fire and electric shock could result. (If carpets, etc., are laid over cords and they go unnoticed, heavy objects could end up placed on them).

* If power supply cords are damaged (core wires are exposed or there is

breakage), contact your dealer to request replacements. Using them in this condition could result in fire and electric shock.

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* Do not place the equipment on unstable tables or on inclined surfaces or other

unstable places, as the equipment could be dropped or fall, resulting in injury.

* Do not damage, process, bend excessively, twist, pull or heat power supply

cords or they could be damaged, and fire and electric shock could result.

* Do not remodel the equipment or fire and electric shock could result.

* Do not use the equipment within or near a sink or fire and electric shock could

result.

* If thunder can be heard, do not touch the power supply plug or electric shock

could result.

Use in water-accessing places prohibited

Touching prohibited

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* Do not place the equipment in places where hot air and dust are prevalent. Doing

so will not only deteriorate image quality but could cause fire and electric shock.

* Do not block up the equipment's ventilation holes. Doing so will cause the inside

to fill with heat, which could result in fire. Refrain from using the equipment in the following ways.

- With the equipment face up, on its side, or upside down.

- With the equipment placed in lockers, racks, bookcases and other narrow places with poor ventilation.

- With the equipment on surfaces covered by tablecloths or the like.

1. Cautions Prior to Use

* When installing the equipment, leave at least 20cm between it and any wall. Also,

to facilitate heat radiation, leave sufficient space between the equipment and other devices or the interior could become hot, resulting in fire.

* Do not sit on the equipment. Falling, breakage, and injury could result.

* Do not place heavy objects on the equipment. If balance is lost, falling, dropping,

and injury could result.

* Do not stare for a long time at the light that is emitted by the LED light source.

Doing so for a short time will not result in any problem, but doing so for a long time could result impair your vision.

* Do not have the power supply cord near heating apparatuses. The cord's

covering could melt, resulting in fire and electric shock.

* When unplugging the power supply plug and connection cords from electrical

outlets, do not pull the cord itself or it could be damaged, resulting in fire and electric shock. Make sure to grasp the plug itself when unplugging a cord.

1. Cautions Prior to Use

* Do not plug in or unplug power supply cords with wet hands or electric shock

could result.

* If the power supply plug is damaged or contact with the electrical outlet is not

secure, do not use the equipment or electric shock, short circuiting, and outbreak of fire could result.

* When moving the equipment, make sure to first turn OFF the power supply

switch, unplug the power supply plug from the electrical outlet, and unplug any connecting cables between devices.

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* When carrying the equipment, do not subject it to shock or malfunction could

result.

* When making repairs, for safety purposes, make sure to unplug the power supply

plug from the electrical outlet or electric shock could result.

* If the equipment will not be used for a long time, for safety purposes, unplug the

power supply plug from the electrical outlet or fire could result.

* When installing the camera head to the dark box, use the dedicated screw holes

and screws, fixating the dark box in 4 places. When installing the camera head to something other than the dedicated dark box, do not use screws over 10mm in the fixating holes for the screws. Do not conduct installation with methods other than those instructed herein or damage to the equipment and malfunction could result.

This caution does not apply to the LAS-1000plus.

* Using dedicated cables, make secure connections to the camera head, dark box,

analyzing unit personal computer, and power supply. Imperfect connection and connections made with other than the dedicated cables could result in incorrect operation and malfunction. When connecting and disconnecting cables, do so with the power OFF or incorrect operation and malfunction could result. Do not bend cables in excess, bundle them or place heavy items on them or incorrect operation and malfunction could result.

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* Never touch the shutter of the camera head. If the shutter becomes deformed,

correct operation will not be possible.

This caution does not apply to the LAS-1000plus.

* Do not install anything other than the attached lens and attached C-mount

adapter. When using a C-mount lens, use the dedicated C-mount adapter. If an adapter other than the specified is used, malfunction could result.

This caution does not apply to the LAS-1000plus.

1. Cautions Prior to Use

* Do not connect anything other than the dedicated light source to the dark box's

internal connectors.

* Do not throw the camera head and/or camera controller into fire.

Explosion could result in injury or burns.

* Do not unnecessarily turn the equipment's power switch ON/OFF repeatedly or

malfunction could result.

* Do not leave exposure specimens in the dark box or sample tray or malfunc-

tion could result.

2. Usage Cautions

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2.1 Place of Installation

So that we may have you use the equipment normally and safely, install it in places such as the following.

* Places not subjected to direct sunlight or other strong light.

If necessary, shield out light using curtains, blinds, and the like. The presence of strong light can cause fogging*.

* Fogging: The phenomenon in which light leakage and other unintended light accumu-

late on recorded images.

* Places that can support the weight of the equipment, are subjected to

little vibration, and are level and stable.

* Places with good ventilation and little dust.

Note :

In dusty environments, dirt and/or unevenness may appear on image. Moreover, cooling capacity can deteriorate.

* Places in which temperature does not suddenly change.

Sudden heating of a cold room or moving the equipment from a low- to high-temperature place can cause water droplets (dew condensation) to form inside the equipment, resulting in incorrect shutter operation, and quality deterioration, such as image blurring.

* Places with no nearby water faucets, hot-water heaters, warmers,

coolers, heaters, stoves, etc. (high-temperature, high-humidity or lowtemperature, low-humidity places).

* Recommended usage environment*

Temperature: +18oC ~ +28oC Humidity: 35%RH ~ 70%RH (with no dew condensation)

* To elicit satisfactory performance, we recommend using the equipment in the

environments described above.

Using the equipment under high temperatures causes cleaning system startup to take longer and is a cause of errors.

* Avoid having objects nearby that emit strong magnetism (motors,

transformers, televisions, speakers, magnets, etc.), or incorrect operation could result.

* When installing this equipment, leave at least 20cm between it and any

walls. Also, to facilitate heat radiation, leave sufficient distance from other devices. Otherwise, heat could built up inside, resulting in fire.

* Do not place objects on top of the equipment or errors and malfunction

could result.

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2. Usage Cautions

2.2 Power Supply * Conduct in the power supply under stable voltage and use it for the dedicated power supply.

* Make sure to directly connect the power supply plug to a wall outlet

(tripolar plug socket) that has a ground terminal. Extension using table taps or putting many loads on one electrical outlet could result in fire and electric shock.

* Do not wire from the same power supply used by air conditioners,

centrifuges, and other large devices or incorrect operation could result.

* Conduct the following inspections periodically to make sure of the

following:

- The power supply plug is inserted firmly in the electrical outlet.

- The power supply plug and cord do not become abnormally hot.

- The power supply cord does not have cracks or scratches in it.

2. Usage Cautions

2.3 Operational and Maintenance Cautions

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Operational Cautions

* Do not perform any operations that are not described in the Operation

Manual.

* Turn the power supply for the controller OFF after first quitting the

reading software. Turning the power OFF without first quitting the reading software could damage the cleaning system.

* Use the following procedure when unplugging the power supply plug

from the electrical outlet. (1) Quit the reading software.

(2) Press the power supply switch OFF. (3) Unplug the power supply plug.

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3. LED Light Source

LED Used

Blue-, Green-, Red- and White-LEDs are used in the LAS-1000plus.

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Part

LAS-1000plus System Configuration

1. LAS-1000plus System Hardware Configuration

Camera head

Camera controller

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MO drive Analyzing unit LBP

Intelligent dark box II

(IDXII)

Fig. 2.1 Hardware Configuration (Example: Macintosh version)

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2. Specifications

2.1 Performance Characteristics (1) CCD: 1,300,000-pixel cooling CCD camera Effective pixels; 1,384(H) X 922(V)-pixels (On-chip micro lens is used.)

(2) Cooling temperature: -60o Celsius from ambient temperature Fixed

at -25oC when the Image Reader LAS-1000 Lite is used.

(3) Reading noise: 20e- (rms) typ.

(4) Dark current noise: 0.01e-/pix.sec or less

2.2 External Dimensions and Weight

(5)

Number of gradations

: At time of image recording:

14 bits (16 bits after shading correction) At time of focalization: 8 bits

(6) Exposure time: 1/100sec - 3600sec; bulb exposure

(7) Focusing: Approx. 4 frames/sec

(8) Shading correction: Software system

(9)

Lens distorsion correction

: Software system

(10)Angle of field: 12cmx8cm - 25cmx25cm

(when the URF20L lens is used.)

(11)Dynamic range: 3.7 orders

(12)

Maximum image capacity

Unit

Camera head

: 2.5MB (full-size images) / 1 image

Dimensions (Width X Depth X Height)

200mm X 170mm X 170mm

Weight

3.8 kg

Camera controller

Dark box

Image input unit

120mm X 370mm X 330mm

430mm X 430mm X 750mm

* Tray and lens are not included. (Lens weight : 0.8 kg)

33.4 kg *

6.5 kg

2. Specifications

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2.3 Power Requirements (1) Input voltage: 100-120 VAC+10% / 200-240 VAC -10% +5% (2) Phase: Single (with 3P grounding pole) (3) Power frequency: 50/60Hz (4) Power consumption: 0.3kVA

2.4 Physical Environment (1) Operating Environment

* Temperature: 15 to 30oC( T<10oC/h) * Humidity: 35 to 70% RH (no dew condensation)

(2) Non-operating Environment

* Temperature: -10 to 60oC * Humidity: 20 to 90% RH (no dew condensation)

(3) Transit/Storage Environment

* Temperature: -25 to 70oC * Humidity: 5 to 95% RH (no dew condensation)

2001. Oct. Version 2.0

Part

Software Installation

1. Introduction / 2. Software Installation and Uninstallation

2001. Oct. Version 2.0

1. Introduction

2. Software Installation and Uninstallation

2.1 Software Installation (for Windows)

Image Reader LAS-1000 Lite is software exclusively for the LAS-1000plus system. Image Reader LAS-1000 Pro is available for both the LAS-1000C and LAS1000 systems. (Image Reader LAS-1000 Lite cannot be used for these systems.)

1 Insert the installer CD-ROM (Image Reader LAS-1000 for Windows and

Macintosh) into the CD-ROM drive.

2 Activate located in the Control Panel and click .

Double-click this icon.

Click here.

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2. Software Installation and Uninstallation

3 A dialog as the one shown below will appear. Click the

button.

4 Carefully read the messages, warnings, etc. appearing in the Welcome

dialog below and click the button to proceed to the Software License Agreement dialog.

5 Carefully read details of the agreement appearing in the Software

License Agreement dialog below. If you agree, press the button to proceed to the Information dialog.

2. Software Installation and Uninstallation

6 In the Information dialog shown below, click the button to

proceed to the User Information dialog.

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7 In the User Information dialog shown below, enter your name and

company name and then click the button to proceed to the Choose Destination Location dialog.

8 To change the default destination for software installation, click the

button in the Choose Destination Location dialog shown below and proceed to the Choose Folder dialog. Proceed to step 10 if the default destination need not be changed.

2001. Oct. Version 2.0

2. Software Installation and Uninstallation

9 In the Choose Folder dialog shown below, specify destination for

installation of the Image Reader LAS-1000 software, and press the

button. The display will then return to the Choose Destination

Location dialog.

10 In the Choose Destination Location dialog shown below, click the

button to proceed to the Setup Type dialog.

User's Tips:

The default folder setting is: C:¥Prog...¥Fujifilm¥Image Reader

11 In the Setup Type dialog shown below, select type of the intelligent dark

box (IDX) actually connected to the system. Select the LAS-1000plus LAS-1000 -> If the IDX is connected, only Image Reader Pro will

LAS-1000plus -> If the IDXII is connected, both Image Reader Pro LAS-1000mini -> If the IDXII is connected, both Image Reader Pro

be installed. and Image Reader Lite will be installed. and Image Reader Lite for LAS-1000mini will be

installed.

2. Software Installation and Uninstallation

12 In the Select Program folder dialog shown below, enter program folder

name for registering the startup menu and click the button to proceed to the Start Copying Files dialog.

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13 In the Start Copying Files dialog shown below, confirm the User

Information, Setup Type and the Destination Directory (destination for installation of this software), and then click the button. Software installation will begin.

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2. Software Installation and Uninstallation

14 When software installation is completed, the following Setup Complete

dialog will appear. Select items as necessary or click the button to complete installation.

2. Software Installation and Uninstallation

2.2 Software Uninstallation 1 Open the Control Panel and double-click .

2001. Oct. Version 2.0

2 Select "Image Reader LAS-1000" in the dialog shown below and click

the button.

+ 109 hidden pages

FUJIFILM LAS-1000plus Operation Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Read this first

Contents

1. Cautions Prior to Use

2. Usage Cautions

Operational Cautions

3. LED Light Source

LED Used

1. LAS-1000plus System Hardware Configuration

2. Specifications

1. Introduction

2. Software Installation and Uninstallation