FujiFilm BAS-2500 User Manual

Science Imaging Systems

High-performance

Multi-discipline

Imaging Analysis

BAS-25OO

hether you are in the life sci-

ences or physical sciences,

today’s scientific investiga-

tions require that your imaging

W

handle multiple applications and provide

data that is far more precise and reproducible

than ever before. Fujifilm’s BAS-2500 was

developed with this in mind.

Perhaps your current research calls for elec-

trophoresis, blots and macro arrays. Then,

six months from now, in-situ hybridization

receptor binding assays. Or thin layer chro-

matography. Whole body autoradiography.

That is no problem for the BAS-2500.

system be versatile enough to

Multiple-application versatility
with imaging performance 100x
greater than X-ray film.

Perhaps you are in the physical and material

sciences and your imaging calls for X-ray

crystallography. Semiconductor wafer check-

ing. And, later, non-destructive testing.

Again, the BAS-2500 will perform for you.

Regardless of discipline of application, most

scientists agree, the better your imaging the

more likely your efforts will lead to insight

and discovery. This is why the BAS-2500,

incorporating Fujifilm’s patented phosphor

Imaging Plate (IP), is an invaluable part of

every laboratory.

The BAS-2500 Scanner’s
speed and accuracy equal
unparalleled throughput

The BAS-2500 is the system of choice for

high-density DNA hybridization arrays. It

provides spatial resolution adequate for

many tissue studies in pharmacokinetics and

toxicology. With a dynamic range up to five

orders of magnitude, incomparable image

quality is guaranteed.

With a Fujifilm BAS-2500, a

complete 20 x 40 cm imag-

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ing plate can be scanned within five minutes

at 16 bits. And this high throughput is

achieved without sacrificing superior sensi-

tivity and quantitative accuracy.

The BAS-2500’s range of recommended

applications includes Molecular Biology

(1D electrophoresis, 2D electrophoresis,

DNA & Protein blots and Macro Arrays);

Pharmacokinetics & Toxicology (whole

body autoradiography and thin layer chro-

matography); and Physical and Material

Structural Analysis (X-ray crystallography,

semiconductor wafer check and non-

destructive testing).

Reusable Imaging Plate
is 100x more sensitive
than X-ray film

The Fujifilm IP is a reusable two-dimen-

sional sensor for the detection and storage of

ionizing radiation energy in photostimulable

phosphor crystals. Fujifilm IPs are approxi-

mately 100 times more sensitive than X-ray

film, and have greater quantitative accuracy.

When Fujifilm IPs and the BAS-2500 sys-

tem replace standard X-ray film and process-

ing, your results are available from 10 to 100

times faster. And because Fujifilm IPs are

more sensitive than X-ray film, they capture

information unobtainable using X-ray film

autoradiography. And all of these advantages

can be enjoyed without the need for process-

ing chemicals or a darkroom. While other

phosphor imaging systems have appeared,

Fujifilm scanners and imaging plates remain

the standard by which others are measured.

Fully networkable

All Fujifilm research imaging systems

are easily networked for

seamless integration into

multi-user laboratory envi-

ronments. Ask your repre-

sentative for details.

The BAS-2500 incorporates Fujifilm’s

proprietary light guide and is manufactured to

ISO 9002 standards for maximum efficiency

and quality.

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Data Images, left to right:

1. Fast thin film phase and orientation analysis of Ta on Si with an exposure time of 3 seconds. Sample courtesy of Dr. Sabrina Lee, Water vliet Arsenal, Professor Toh-Min Lu and Dr. Donald Windover,

Rensselaer Polytechnic Institute, Troy, N.Y., U.S.A.

2. Dot blot. Sample courtesy of Assistant Professor Kuniya Abe, Kumamoto University, Faculty of Medicine, Institute of Molecular Embryology and Genetics, Department of Developmental Genetics.

3. Thin film phase and orientation analysis of Ta on Si with an exposure time of 10 minutes. Sample courtesy of Dr. Sabrina Lee, Watervliet Arsenal, Professor Toh-Min Lu and Dr. Donald Windover,

Rensselaer Polytechnic Institute, Troy, N.Y., U.S.A.

4. Whole-body rat slice. Fujifilm file image.

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Specifications and Applications

Specifications

Imaging

IP Size 20 x 40 cm

Pixel Size 50/100 µm

Reading Time 5 min. (50µm)

Detection Limit

Dynamic Range 4/5 orders of magnitude

Gradation 65,536 (16 bits)/256 (8 bits) selectable

Shading ± 5% over entire scan area

Imaging Plates (see details below)

BAS-MS2040, BAS-SR2040, BAS-TR2040, BAS-ND2040

Dimensions and Weight

Dimensions 980 mm (w) x 450 mm (H) x 590 mm (D)

Weight 62 kg

Image Reading Software

ImageReader (MacOS/Windows®95, Windows®98, Windows®NT ver. 4.0)

Image Analysis Software

Science Lab (MacOS/Windows®95, Windows®98, Windows®NT ver. 4.0)

32

P

0.11 dpm/mm2/hr

14

C

0.90 dpm/mm2/hr

Applications

Life Science

Genomics and
Proteomics Imaging

Physical and

Structural Analysis

Material
Sciences

Environmental
Monitoring

● Recommended ● Available

Autoradiography

Dosimetry

Molecular Biology

Neuroanatomy

Neurophysiology

Immunology & Cell Biology

Pharmacokinetics&Toxicology

X-Ray Crystallography

Semiconductor Wafer Check

Non-Destructive Testing

Imaging Plates vs. X-ray Film

1D Electrophoresis

2D Electrophoresis

DNA & Protein Blots

Macro Arrays

In-Situ Hybridization

Receptor Binding Assays

Whole Body Autoradiography

Thin Layer Chromatography

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Imaging Plates Size: 2040 (20cm x 40cm)

The sensitivity of Fujifilm’s patented IP provides a highly

efficient, uniform and sensitive detection system far superior to

BAS-MS Designed for compatibility of high sensitivity and water-

resistance. For use with all existing BAS.

BAS-SR Designed with blue pigment for both optimum sharpness

(especially for 50µm or smaller pixel size) and good wet-

sample durability.

BAS-TR Designed for highest resolution tritium detection, with blue

pigment and no surface-protection layer.

For use with dry samples.

BAS-ND Designed for neutron detection, with blue pigment, surface-

protection and Gd203converter in the photostimulable layer.

Good wet-sample resistance.

that of X-ray film. All Fujifilm IPs, except BAS-TR (tritium

detection) IPs, are reusable and there is no need for a darkroom

or development and fixing chemicals. There are Fujifilm IPs for

virtually any type of emitter, all with superior accuracy.

Additionally,

Fujifilm IP images

can be repeatedly

scanned before

erasing the IP

for your next

experiment.

A comparison of Fujifilm Imaging Plate

versus X-ray film.

Fuji Photo Film Co., Ltd. 26-30, Nishiazabu 2-Chome, Minato-ku, Tokyo 106-8620, Japan, Tel: [phone], Fax: [phone] • http://home.fujifilm.com/info/products/science/index.html • E-mail: sg info @tokyo.fujifilm.co.jp

Fujifilm Medical Systems U.S.A., Inc. 419 West Avenue, Stamford, CT 06902, U.S.A. Tel: [phone] ext. 6112 (1-800-431-1850 ext. 6112 in the U.S.) Fax: [phone] • http://www.fujimed.com • E-mail: [e-mail]

Fuji Photo Film (Europe) GmbH, Heesenstr. 31, 40549 Düsseldorf, Germany, Tel: [phone] Fax: [phone] • http://www.fujifilm.de • E-mail: [e-mail]

Specifications and system configuration subject to change for improvement without notice. All other product names mentioned herein are the trademarks of their respective owners.

Ref. No. BB-901E (01,06)