Fisher EV200 User manual

Operating Manual

Electrophoresis power supplies Model EV202

EV215 EV231 EV232 EV233 EV243 EV261 EV262

EV265

Very important

This electrophoresis power supply is a high technology instrument available in several versions. As it is capable of giving dangerous voltage levels by which high power is involved, we suggest that you take a few moments to read this manual thoroughly. Although this instrument is equipped with all necessary safety features against abuse and other accidental failures, caution should be exercised when working with high voltage equipment.

• Avoid to touch the outlets with any conducting object and make sure there is a second person present for your safety in case of any severe electric shock.

• Never touch any part of the assembly (power supply, leads or tank) before hav ing switched off.

• Never manipulate with wet hands.

• Do not ground any of the outputs or the buffer in the tank.

• Connect the outlets only to an insulated electrophoresis tank with safety cover.

• Never make any other connections, such as e.g. putting several power supplies in series or in parallel.

• In order to prevent electric shock, never open the back plate nor remove the cover.

• Do not expose the unit to rain or any other liquid.

• Do not spill liquid or insert metal objects inside the unit.

• Take care so that the power supply is not dropped to avoid damaging the cabi net which defeats safeguards or injuring yourself.

• If the unit has been dropped or the cabinet has been damaged, unplug it and have it checked by an authorised service technician to restore the safe guards.

• The fact that the unit operates satisfactorily does not imply that the unit is properly earthed or that it is completely safe. If in any doubt about the effec tive earthing of the unit, contact a qualified electrician.

• Never block the ventilation holes or place the unit in any enclosure unless proper ventilation is provided.

• Never place the unit near or over a radiator, heat register or stove.

• Avoid locations where the instrument is exposed directly to the sun light.

This instrument is manufactured with the latest technology and needs no particular maintenance. Consort certifies that this instrument was thoroughly inspected and tested at the factory prior to shipment and found to meet all requirements defined by contract under which it is furnished. However, dimensions and other physical characteristics may differ.

The normal operating temperature should be between 0° and 40°C. Never use the instrument in a room with high humidity (>95 %) or at very low temperatures (condensation water!).

Connect the instrument only to an earthed power line. The required power source is indicated on the label at the back of the instrument. Do not cut and splice the power cord. When removing the power cord from the wall outlet, be sure to unplug by holding the plug attachment and not by pulling the cord. Do not hold the plug by wet hand.

BIOTEC-FISCHER GmbH Tel.: +49-6408-6072 Daimlerstrasse 6 Fax: +49-6408-64165 35447 Reiskirchen e-mail: info@biotec-fischer.de GERMANY

This instrument (excluding all accessories) is warranted against defective material and workmanship for a period of thirty-six (36) months from the date of shipment ex factory. Consort will repair all defective equipment returned to it during the warranty period without charge, provided the equipment has been used under normal laboratory conditions and in accordance with the operating limitations and maintenance procedures in this instruction manual and when not having been subject to accident, alteration, misuse or abuse. A return authorisation must be obtained before returning any product for warranty repair on a freight prepaid basis!

BIOTEC-FISCHER or Consort is not liable for consequential damages arising out of the use or handling of its products.

Introduction

Contact

Warranty

In the event of this instrument being returned for servicing, the owner is requested to remove the power supply lead and NOT to send the following items unless they are suspect:

Manual Cables Accessories If serious malfunctioning occurs, stop using the unit immediately and consult your

local dealer.

éê = Button for entering a value or for selecting a function.

SET/ENTER RUN/STOP

= Button for programming the desired parameters.

= Button to start or stop an experiment.

Servicing

Keyboard

SET-UP

1. 1. Select SET-UP and press SET.

2. Select LANGUAGE and press SET.

3. Select the desired language and press SET.

4. Select CONTRAST and press SET.

5. Adjust the contrast of the display and press SET.

6. Select ALARM and press SET.

7. Decide whether or not the output voltage should shut down at a too

low output current and press SET.

8. Select POWER FAIL and press SET.

9. Decide whether or not the instrument should proceed with the

experiment for the remaining time after a power failure and press SET.

10. Select STAND-BY and press SET to return.

• Select INFO and press SET to see general information about the electrophoresis power supply.

---- STAND-BY ---SET-UP

---- SET-UP ---LANGUAGE

CHOOSE LANGUAGE:

ENGLISH

---- SET-UP ----

CONTRAST

---- SET-UP ----

ALARM

CURRENT<1mA

ALARM: YES

---- SET-UP ----

POWER FAIL

DETECTION: YES

---- SET-UP ----

STAND-BY

---- SET-UP ----

INFO

Method programming permits to store up to 9 different methods, each with 9 steps, of frequently used parameters in the non-volatile memory for future recall. Each step is able to recall a next one, providing a flexible multiple step function for special techniques. The method mode also permits to program a linear voltage gradient for any step provided the limiting current or power is not attained. Parameters can be changed temporarily without interrupting the run.

1. Verify if the instrument is switched off.

2. Connect the electrophoresis tank to the instrument.

3. Switch the instrument on.

4. Select the METHOD mode and press SET.

5. Select the desired method and press SET.

6. Select the desired step and press SET.

7. Decide whether or not a voltage gradient has to be performed and press SET.

8. Enter the desired voltage limit and press SET (= the voltage end point in case of a gradient step).

9. Enter the desired current limit and press SET (this limit is automati cally set to maximum and cannot be changed in case of a gradient step).

10. Enter the desired power limit and press SET (this limit is automati cally set to maximum and cannot be changed in case of a gradient step).

11. Enter the desired time-units (h of kVh) and press SET.

12. Enter the desired time and press SET.

13. Decide if the experiment should automatically proceed with a next step (resuming from point 6) or stop after this step. Enter the desired procedure and press SET.

14. Press RUN to start the experiment.

• At any time, you can return in the stand-by mode by holding SET pressed during 2 seconds.

• At any time, you can avoid programming unnecessary parameters. To do so, simply press RUN instead of SET after entering the last relevant value and the instrument will immediately start the exper iment keeping the other parameters to their previous values.

• To ensure that the desired parameter is kept constant, we suggest to pre-set all other parameters to maximum.

• A blinking unit of the controlling parameter appears when the regu lation is complete.

• The timer will always count down.

If the timer is still running after switching on, the power supply will automatically proceed with the experiment for the remaining time. Press STOP if you want to go in stand-by during the run

Method Programming

STAND-BY

METHOD

ENTER 1 ∫ 1 METHOD: 1

ENTER 1 ∫ 1 STEP: 1

ENTER 1 ∫ 1 GRADIENT: NO

ENTER 1 ∫ 1 VOLTAGE: 400V

ENTER 1 ∫ 1 CURRENT 300mA

ENTER 1 ∫ 1 POWER 50W

ENTER 1 ∫ 1 TIME UNIT h

ENTER 1 ∫ 1 TIME 99:59h

ENTER 1 ∫ 1 END METHOD: YES

time

STEP 2 STEP 3 STEP 4 STEP 5

RUN 1 ∫ 1 99:59h 400V 300mA 50W

Manual Programming

Manual programming permits to set voltage, current, power and time limits for a simple routine electrophoresis run. Parameters can be changed temporarily without interrupting the run.

1. Verify if the instrument is switched off.

2. Connect the electrophoresis tank to the instrument.

3. Switch the instrument on.

4. Select the MANUAL mode and press SET.

5. Enter the desired voltage limit and press SET.

6. Enter the desired current limit and press SET.

7. Enter the desired power limit and press SET.

8. Enter the desired time-units (hours or kilovolthours) and press SET.

9. Enter the desired time and press SET (pre-set to [--:--] if no timing is required).

10. Press RUN to start the experiment.

• At any time, you can return in the stand-by mode by holding SET pressed during 2 seconds,

• To ensure that the desired parameter is kept constant, we suggest to pre-set all other parameters to maximum.

• A blinking unit of the controlling parameter appears when the regu lation is complete.

• The timer will always count down. However, when no time limit has been programmed it will count up.

• If the timer is still running after switching on, the power supply will automatically proceed with the experiment for the remaining time. Press STOP if you want to go in stand-by during the run.

only the parameters or the running step can be changed. A gradient step can never be changed.

1 . Press SET to change temporarily the parameters without interrupting

the run.

2. Change the actual voltage limit and press SET.

3. Change the actual current limit and press SET.

4. Change the actual power limit, and press SET.

5. Change the actual time and press SET.

6. The power supply will now continue the run.

• Temporarily changed parameters are not been kept in memory!

• The instrument will automatically return to the run mode when no button has been pressed during 10 seconds.

---- STAND-BY ---MANUAL

ENTER VOLTAGE 400V

ENTER CURRENT 300mA

ENTER POWER 50W

ENTER TIME UNIT h

ENTER TIME 99:59h

RUN 400V 300mA 50W

Temporarity changing parameters

SET 1 ∫ 1 99:59h 400V 300mA 50W

Data Logging

Select SET-UP and press SET.

2. Select DATA and press SET.

3. Select DURATION and press SET.

4. Select the desired time for the data-logging and press SET.

5. Select LOG and press SET.

6. Decide whether or not all data (voltage, current, power, time) during the next experiments should be logged and press SET.

7. Select SET-UP and press SET to return.

• Starting a new experiment will erase the previous data in memory.

• Stored data can be transferred to a computer via the RS232 output using optionally available software.

• At any time, you can return in the stand-by mode by holding SET pressed during 2 seconds.

---- STAND-BY ---SET-UP

---- SET-UP ----

DATA

---- DATA ----

DURATION

LOG DATA FOR: 10h

---- DATA ---LOG

LOG DATA: YES

---- DATA ----

SET-UP

Alarms

Ground leakage A dangerous ground leakage is present (check

thoroughly the complete assembly). Your power supply is NOT defective! See Frequently Asked Questions at the end of this manual.

Short Circuit Connected tank is short-circuited or has a very low

resistance (check thoroughly the complete assembly). Your power supply is NOT defective! See Frequently Asked Questions at the end of this manual.

Overload Connected tank has a too low resistance (check

specifications). Your power supply is NOT defective! See Frequently Asked Questions at the end of this manual.

Current <1mA The output current is lower than 1 mA (switch the alarm

off in the SET-UP menu). Your power supply is NOT defective! See Frequently Asked Questions at the end of this manual.

Call Service Failure in the electronics. See Support on

www.consort.be.

You could also try a general RESET: switch the instrument on while holding SET pressed. Attention! All stored programs and data will be erased!

*** ERROR ***

GROUND LEAKAGE

*** ERROR ***

SHORT CIRCUIT

*** ERROR ***

OVERLOAD

*** ERROR ***

CURRENT <1mA

*** ERROR ***

CALL SERVICE

Mode d’emploi

Electrophoresis power supplies Model EV202

EV215 EV231 EV232 EV233 EV243 EV261 EV262

EV265

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