41 Eagle Road
Danbury, CT 06813-1961 USA
(203) 796-0400
http://sonifier.com
Manual Change Information
At Branson, we strive to maintain our position as the leader in ultrasonics plastics joining,
metal welding, cleaning and related technologies by continually improving circuits and
components in our equipment. These improvements are incorporated as soon as they are
developed and thoroughly tested.
Information concerning any improvements will be added to the appropriate technical
documentation at its next revision. Therefore, when requesting service assistance for
specific units, note the Revision information found on the cover of this document.
Sonifier is a registered trademark of Branson Ultrasonics Corporation.
Loctite is a registered trademark of Henkel Corporation, U.S.A.
UL is a registered trademark of Underwriters Laboratories.
CSA is a registered trademark of CSA International.
Other trademarks and service marks mentioned herein are held by their respective
owners.
ii100-412-194 REV. 01
Foreword
Congratulations on your choice of a Branson Ultrasonics Corporation system!
The Branson Sonifier
product using this sophisticated technology for a variety of customer applications. This
Instruction Manual is part of the documentation set for this system, and should be kept
with the equipment.
Thank you for choosing Branson!
®
ultrasonic cell disrupter/homogenizer is the newest generation of
Introduction
This manual is arranged into several structured chapters which will help you find the
information you need to know to safely handle, install, set up, program, operate, and/or
maintain this product. Please refer to the Table of Contents and/or the Index of this
manual to find the information you may be looking for. In the event you require additional
assistance or information, please contact our Product Support department (see 1.4 How to
Contact Branson for information on how to contact them) or your local Branson
This chapter contains an explanation of the different Safety Notice symbols and icons
found both in this manual and on the product itself and provides additional safety
information for ultrasonic processing. This chapter also describes how to contact Branson
for assistance.
1.1.1Symbols Found in this Manual
These symbols used throughout this manual warrant special attention:
WARNINGGeneral Warning
Warning indicates a hazardous situation or practice which, if not
avoided, can result in serious injury or death.
WARNINGHigh Voltage Hazard
High voltage. Turn power off before servicing.
WARNINGCorrosive Material Hazard
Corrosive material. Avoid contact with eyes and skin. Wear proper
protection.
CAUTIONGeneral Warning
Caution indicates a hazardous situation which, if not avoided, could
result in minor or moderate injury.
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CAUTIONLoud Noise Hazard
Loud noise hazard.
NOTICE
Notice is used to address practices not related to personal injury. It contains important
information. It might also alert the user to unsafe practices or conditions that can
damage equipment if not corrected.
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1.1.2Symbols Found on the Product
Familiar gr aphic warning symbols are used to alert the user to items of concern or hazard.
The following warning symbols appear on the Sonifier power supply.
Figure 1.1 Safety Label on the Rear of the Sonifier Power Supply
Figure 1.2 Safety Label on the 102C Converter
4100-412-194 REV. 01
1.2General Precautions
Observe the following safety considerations when operating the Sonifier cell disruptor
system:
CAUTIONGeneral Warning
• Make sure that the equipment is properly grounded. DO NOT operate if it
is not.
• The unit is equipped with a three-conductor cord and three-prong
grounding-type plug, and must be plugged into a three-prong groundingtype wall receptacle. DO NOT under any circumstances remove the power
cord ground prong.
• DO NOT operate the equipment with the cover removed. High voltage is
present within the equipment when connected.
• DO NOT turn on the ultrasonics without the converter and horn or tip
attached.
• DO NOT touch the horn or tip when ultrasonics are active. When
handling, removing, or attaching a horn or tip, be sure that the ON/OFF
switch on the back of the unit is set to OFF. T ouching the horn or tip while
the unit is ON can result in serious personal injury.
• DO NOT allow the horn or microtip to contact lab stands, beakers, test
tubes or similar objects. Microtip failure may result. Breakage of
glassware may result in the loss of a specimen.
• DO NOT operate the equipment at more than 70% amplitude when using
a microtip.
• Appropriate eye protection should be worn to prevent possible splash
injury.
CAUTIONLoud Noise Hazard
Sound level and frequency of the noise emitted during the ultrasonic
processing may depend upon the specific type of application.
Some parts vibrate at an audible frequency during the process. Some
or all of these factors may result in an uncomfortable noise being
emitted during processing.
In such cases operators may need to be provided with personal
protective equipment. See 29 CFR (Code of Federal Regulations)
1910.95 Occupational Noise Exposure.
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1.2.1Intended Use of the System
The Sonifier ultrasonic cell disrupter/homogenizer can be used to disrupt cells, bacteria,
spores, or tissue, and are ideal for initiating and accelerating chemical, biochemical, and
physical reactions, and for degassing liquids. With the Sonifier system, you can prepare an
emulsion to 0.01 micron, homogenize immiscible liquids, polymerize some materials, and
depolymerize others.
1.2.2Setting up the Workplace
Measures for setting up a workplace for safe operation of the Sonifier system are outlined
in Chapter 5: Installation and Setup.
1.2.3Regulatory Compliance
This product meets electrical safety requirements and EMC (Electromagnetic Compliance)
requirements for North America and the European Union.
All units comply with WEEE/RoHS requirements.
6100-412-194 REV. 01
1.3Warranty
Refer to the “Terms and Conditions of Sale” found on the back of your Invoice for
information about the Warranty issued for your Branson products. If you have any
questions, please contact your Branson representative. The product warranty information
is summarized below.
WARRANTY
When used in accordance with written instructions and under normal operating conditions,
Branson Ultrasonics Corporation equipment is guaranteed to be free of defects in
MATERIAL and WORKMANSHIP for 3 y ears from the date of original delivery by Br anson or
by an authorized representative. Any unit which proves defective during the stated period
will be repaired free of charge or replaced at the sole discretion of Branson Ultrasonics
Corporation, F.O.B. Danbury, Connecticut, U.S.A. or an authorized repair station as
advised by Branson, provided the defective unit is returned properly packed with all
transportation charges prepaid. A limited warranty as specified may apply to certain
components of the equipment.
WARRANTY EXCEPTIONS
This warranty shall not apply to equipment subjected to misuse, improper installation,
alteration, neglect, accident or improper repair.
This warranty is limited to the original purchaser and is not transferable.
Horns and tips fabricated by Branson for use in equipment described in this manual are
manufactured to exacting parameters. Using altered or modified horns and tips or horns
and tips otherwise unqualified by Branson can produce undue stresses that may damage
the equipment. Equipment failures resulting from using unqualified horns and tips are not
covered by the Branson warranty.
Microtips are designed to give maximum mechanical energy output. Since they operate
close to the stress limits of titanium, Branson cannot guarantee microtips against failure.
CONTACT YOUR BRANSON REPRESENTATIVE OR BRANSON ULTRASONICS
CORPORATION, DANBURY, CONNECTICUT, SHOULD YOU HAVE ANY QUESTIONS
CONCERNING HORN QUALIFICATION.
100-412-194 REV. 017
1.4How to Contact Branson
The mailing address and telephone information for Branson is as follows:
Tell the operator which product you have and which person or department you need. If
after hours, please leave a voice message with your name and return telephone number.
1.4.1Before Calling Branson for Assistance
This manual provides information for troubleshooting and resolving problems that could
occur with the equipment (see Chapter 7: Maintenance). If you still require assistance,
Branson Product Support is here to help you. The following questionnaire lists the
common questions you will be asked when you contact the Product Support department,
to help identify the problem.
Before calling, determine the following information:
1. Your company name and location
2. Your return telephone number
3. Have your manual with you. If troubleshooting a problem, refer to
4. Know your equipment model and serial numbers (found on a data label on the units).
Information about the horn (part number, gain, etc.) or other tooling may be etched into the
tooling. Software- or -firmware based systems may provide a software version number, which
may be required. (The Sonifier power supply provides the firmware information during power-up)
5. What horn and accessories are being used?
6. What are the setup parameters and mode?
7. Is your equipment in remotely operated system? If so, what supplies the “start” signal?
8. Describe the problem; provide as much detail as possible. For example, is the problem
intermittent? How often does it occur? How long before it occurs if you are just powering up? If
an error is occurring, which error or message?
9. List the steps you have already taken
10.What is your application, including the materials being processed?
11.Have a list of service or spare parts you have on hand (tips, horns, etc.)
Before sending equipment for repair, provide as much information with the equipment to
help determine the problem with the system. Fill in any details below or on a separate
sheet.
1. Describe the problem; provide as much detail as possible. For example, is this a new problem? Is
the problem intermittent? How often does it occur? How long before it occurs if you are just
powering up?
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
2. Is your equipment in a remotely operated system? If so, is the problem related to Start/Stop
control, or interaction with PLC’s or other devices, etc.?
____________________________________________________________________________
____________________________________________________________________________
3. If the problem is with an external signal or output, which one?
____________________________________________________________________________
____________________________________________________________________________
4. If known, include plug/pin # (e.g., P29, pin #3):
____________________________________________________________________________
5. What are the setup parameters?
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
6. What is your application (e.g., continuous, pulse, temperature, etc.)?
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
7. Name and phone number of the person most familiar with the problem:
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
8. Notify Branson prior to shipping the equipment.
NOTICE
T o return equipment to Br anson, you must first obtain an RGA number from Branson, or
the shipment may be delayed or refused.
9. For equipment not covered by warranty, include a purchase order for the repair costs to avoid
delay
10.Pack carefully in original packing material to avoid damage in shipment
11.Return general repairs by any desired method. Send priority repairs by air freight
12.Prepay the transportation charges FOB Laredo, TX, U.S.A.
Before sending equipment for repair, provide as much information with the equipment to
help determine the problem with the system. Use the previous page to record necessary
information.
NOTICE
T o return equipment to Br anson, you must first obtain an RGA number from Branson, or
the shipment may be delayed or refused.
Americas
Call the Repair department to obtain a Returned Goods Authorization (RGA) number. If
requested, the Repair department can send you a facsimile of the Returned Goods
Authorization form to fill out and return with your equipment.
1. Provide as much information as possible that will help identify the need for repair. Include a copy
of the previous page with your information filled in
2. Carefully pack the equipment in original packing cartons
3. Clearly label all shipping cartons with the RGA number on the outside of cartons as well as on
your packing slip, along with the reason for return
4. Return general repairs by any convenient method. Send priority repairs by air freight
5. You must prepay the transportation charges FOB Laredo, TX, U.S.A.
Europe and Asia
Please contact your Sonifier distributor for information pertaining to service and repair.
10100-412-194 REV. 01
1.6Obtaining Replacement Parts
You can reach Branson Parts Store at the following telephone numbers:
Branson Part Store
Direct telephone number: 877-330-0406
Fax number: 877-330-0404
Many parts can be shipped the same day if ordered before 2:30 p.m., Eastern time.
A parts list is found in Appendix B: Parts Lists of this manual, listing descriptions and part
numbers. If you need replacement parts, coordinate the following with your purchasing
agent:
The Sonifier system is often used by laboratory personnel in the medical and chemical
process fields. The system consists of three core elements: the Sonifier power supply, the
converter and horn. The system can also interface with a temperature probe, and user
custom digital control interface.
Figure 2.1 The Sonifier Power Supply
The Sonifier power supply converts AC line voltage to 20 kHz or 40 kHz electrical energy.
This high-frequency electrical energy is fed to the converter where it is converted to
mechanical vibrations. The heart of the converter is an electrostrictive element which,
when subjected to an alternating voltage, expands and contracts. The converter vibrates
in a longitudinal direction and transmits the motion to the horn tip immersed in the
solution, which causes cavitation.
The implosion of microscopic bubbles or cavities in the solution results, causing the
molecules in the medium to become intensely agitated.
The Sonifier system is a constant amplitude device. As the load or pressure on the horn
face increases, the Sonifier system develops more power to maintain the amplitude for
any given output control setting. When the horn is operated in air, it is subjected to
minimum pressure, and minimum power is required to maintain amplitude.
The load increases when the horn is immersed in a liquid; the more viscous the liquid, the
higher the load and the more power developed. If a flow-through cell that can be
pressurized is used, thereby increasing pressure on the horn, even more power is
developed. For any given application, more power results when a horn of higher amplitude
or larger radiating surface is used, or when any horn is driven at higher amplitude.
14100-412-194 REV. 01
By setting various operation parameters, you can precisely control the way in which
ultrasonics are applied to the samples or liquids for processing. You can:
• Specify the time duration of the ultrasonic processing
• Specify the amount of energy you want to deliver to the sample or liquid during processing
• Adjust the amplitude setting between 10% and 100% of maximum amplitude (microtip 70%
maximum)
• Prevent excessive temperature increase in the sample or liquid by setting ultrasonics to operate
in Pulse mode or pausing the ultrasonic cycle
• Bring a sample or liquid to a desired temperature and hold it there, varying by only a few
degrees, for a desired duration using the Pulse Temperature mode
• Set the maximum allowable temperature in the sample or liquid, so that ultrasonics will stop
automatically when the specified temperature is reached
NOTICE
Some operations require the optional temperature probe.
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2.2Front Panel Controls and Indicators
This section describes the controls that you use to operate the Sonifier power supply.
These controls allow for accuracy and repeatability of control settings. A detailed
description of how and when to use each front panel control, the valid formats for the data
that you enter, and the response you receive from the system when you use each of these
controls is provided in
The Sonifier power supply is equipped with a keypad and LCD on the front panel of the
unit. With the keypad, you can set functional modes of operation and control parameters.
Availability of the v arious functions depends on the control mode or state of the system. If
an error condition exists, the Alarm/Error icon will flash and the beeper will sound three
times.
Some functions of the Sonifier power supply can be controlled through the external input
connector located on the rear of the unit. 2.3 Back Panel Connections describes the back
panel of the unit.
2.2.1Sonifier Power Supply Front Panel
Figure 2.2 Sonifier Power Supply Front Panel Controls
Chapter 6: Operation.
16100-412-194 REV. 01
Table 2.1 Front Panel Controls and Indicators
ReferenceDescription
LCD
The LCD allows for easy navigation, configuration, and for
communicating control settings and results.
The LCD is divided into four sections:
• The first and second sections are used to highlight the current control
mode when running a preset, to select the control mode when configuring
a preset, and to indicate when Time, Energy or Temperature parameters
entries are required
• The third section is used to indicate available parameters for each control
mode and to indicate to which parameter corresponds the value shown on
the LCD bottom section
• The fourth section is used to display and edit parameter and system
configuration register values; to select presets and registers; to display
real time cycle data and overall progress; and to indicate alarms/errors or
that a cycle is running
For a detailed description of the display icons refer to Table 2.2 LCD
Icons.
Up/Down Keys
Press the Up and Down keys to initiate a change to the current control
settings, when the system is displaying the ready screen. These keys
are also used to select system configuration registers and modify
parameter values.
Left/Right Keys
Press the Left and Right keys to select control modes, to move
horizontally between digits, and to move between different result
screens.
Enter Key
Press the Enter key to initiate a change to the current control settings,
when the system is displaying the ready screen. This key is also used
to select system configuration registers and presets, and to accept
control setting changes.
Preset Key
Press the Preset key to select a memory location to save the current
control settings or to recall stored settings. For more information on
saving control settings presets see 6.6 Save/Load Control Setup.
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Table 2.1 Front Panel Controls and Indicators
ReferenceDescription
ESC Key
Press the ESC key to return to the ready screen when modifying
control settings. Any setting modifications that were applied before,
by pressing the enter key, will be saved.
Test Key
Press and hold the Test key to turn on sonics. A test first tunes to the
ultrasonic converter's operating frequency at low amplitude, then
ramps the amplitude to the current setting.
Microtip Key
Press the Microtip key when using a microtip probe. This limits
amplitude to 70%, thus preventing damage to the probe.
Reset Key
Press the Reset key to reset errors and alarms.
Pause Key
Press the Pause key to pause an ultrasonic cycle. Press Pause key
again to resume current cycle.
NOTICE
Pause button is disabled when using a handheld converter.
Start/Stop Key
Press the Start/Stop key to turn ultrasonics on/off. To configure as a
press and hold button, see 6.4 System Configuration Registers.
18100-412-194 REV. 01
2.2.2LCD Description
Figure 2.3 LCD Description
Table 2.2 LCD Icons
ReferenceDescription
Numeric Display
Indicates parameter settings, parameter values, system configuration
register numbers, register settings, and preset numbers.
Continuous Mode Icon
Indicates Ultrasonic energy will be delivered continuously during the
cycle.
Pulse Mode Icon
Indicates ultrasonic energy will be delivered in controlled bursts or
pulses.
Time Mode Icon
Indicates time is the main control parameter.
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Table 2.2 LCD Icons
ReferenceDescription
Joules Icon
Indicates energy is the main control parameter.
Max Temperature Icon
Indicates the cycle is controlled to prevent the temperature from
exceeding a set maximum. When used as a controlled mode it
indicates the cycle will end when a set maximum temperature is
reached.
Pulse Temperature Icon
Indicates the cycle is controlled to adjust the pulse duty cycle (ratio of
On and Off times for pulses) to control the temperature to stay
between the set Pulse and Max temperatures during the cycle.
Temperature Icon
Indicates the temperature sensed by the probe during the cycle or the
resulting temperature at the end of the cycle.
Amplitude Icon
Indicates the set amplitude at the tip of the horn as a percentage of
the maximum available amplitude of mechanical vibrations.
On Time Icon
Used to set the amount of on time in pulsed modes.
Off Time Icon
Used to set the amount of off time in pulsed modes.
Total On Time Icon
Used to set the total amount of pulsed time.
Load Icon
Indicates that the number shown on the numeric display corresponds
to the memory location from which the current control settings are to
be recalled. For more information on saving and recalling control
setting presets see 6.6 Save/Load Control Setup.
Preset Save Icon
Indicates that the number shown on the numeric display corresponds
to a memory location where control settings are to be saved. For more
information on saving and recalling control setting presets see 6.6
Save/Load Control Setup.
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